Rh6CG475500

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009762
Physical Location & Seq
Forward (+)
64461796 .. 64467059
5264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG475500.1

Sequence Viewer

Length: 654 bp
ATGTTTGGTTCATTAAGAATTTCTTCCCAGAATCCAAGAAAGCAGCAGCAGAAGCAAGAACGAGAATGGTTAAACTTGAGTTTTAAGCCAGAGAATTTCCTTCCAGGAGTTGTCATTGGCTTCATTCTTGGGTTGTTGTTGGATTTATCACAACCTGGTAAGGGTTCCTTGAAGTTGAAGAGGAAAAATTTCTCGGAGGGAAAGCCCCAACAGCGAAGTTTGGTCTCGACTAATGGTGATGAAGAGCTTAAAATGGTTCTTGTAGTTAGACGAGACTTAAAGATGACAACTGGAAAAATCGCATCTCAGTGTGCTCATGCTGCCACTGGCATGTATGCAGAACTGATGCAAAGGGCGAACACAGGTTGTGAGTGGGTCAAAGACAGTTCTTGCCGTTGGACCAGATCTTTTTCAAGACCCGCCTTTTCTTTTCTCAAATCCACCGTCAACAAACCACCTCTCAAGTCCAGACCCATCTCTTCTCTCCCGTCGACCCCATCACTCTCAAGGCCGGCTCCTCAGTTGGGTTGTCTTCAATCTCTGCTTCCATTTCACACGGCGGTCTCTTCAGCTCGGCTGACGTCATGCCTCGGCATTGACTCGAGGAGCTCGAGGTCGTTGTCTCAGGAACTAGGTCTCAGTGTGCCGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

217

Amino Acids

24.01

Weight (kDa)

10.44

Isoelectric Point (pI)

64.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTH2 PF01981 83 - 127 3.8e-13 Peptidyl-tRNA hydrolase PTH2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 491
AccII CGCG 1 cut(s) 649
AciI CCGC 3 cut(s) 420, 560, 647
AcsI RAATTY 3 cut(s) 18, 94, 187
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 1 cut(s) 581
AfiI CCNNNNNNNGG 2 cut(s) 161, 524
AgsI TTSAA 4 cut(s) 172, 178, 414, 536
AjnI CCWGG 2 cut(s) 103, 154
AluBI AGCT 3 cut(s) 247, 572, 609
AluI AGCT 3 cut(s) 247, 572, 609
Alw21I GWGCWC 2 cut(s) 316, 611
Alw26I GTCTC 5 cut(s) 229, 267, 568, 627, 641
Ama87I CYCGRG 2 cut(s) 601, 610
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 3 cut(s) 43, 46, 320
ApoI RAATTY 3 cut(s) 18, 94, 187
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
Asp700I GAANNNNTTC 2 cut(s) 22, 188
AspS9I GGNCC 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 2 cut(s) 601, 610
AvaII GGWCC 1 cut(s) 399
BanII GRGCYC 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 524
Bbv12I GWGCWC 2 cut(s) 316, 611
BbvI GCAGC 3 cut(s) 55, 58, 307
BccI CCATC 2 cut(s) 482, 505
BceAI ACGGC 2 cut(s) 378, 573
BciT130I CCWGG 2 cut(s) 105, 156
BcoDI GTCTC 5 cut(s) 229, 267, 568, 627, 641
BfaI CTAG 1 cut(s) 632
BglII AGATCT 1 cut(s) 404
BisI GCNGC 4 cut(s) 44, 47, 321, 647
BlsI GCNGC 4 cut(s) 45, 48, 322, 648
Bme1390I CCNGG 2 cut(s) 105, 156
Bme18I GGWCC 1 cut(s) 399
BmeT110I CYCGRG 2 cut(s) 601, 610
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 2 cut(s) 166, 516
BmrFI CCNGG 2 cut(s) 105, 156
BmsI GCATC 2 cut(s) 311, 336
BpiI GAAGAC 1 cut(s) 524
BpuEI CTTGAG 3 cut(s) 97, 446, 490
BsaHI GRCGYC 1 cut(s) 581
BsaI GGTCTC 3 cut(s) 229, 568, 641
BsaJI CCNNGG 1 cut(s) 589
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 524
Bse118I RCCGGY 1 cut(s) 511
Bse1I ACTGG 2 cut(s) 295, 331
BseBI CCWGG 2 cut(s) 105, 156
BseDI CCNNGG 1 cut(s) 589
BseLI CCNNNNNNNGG 2 cut(s) 161, 524
BseMII CTCAG 4 cut(s) 320, 533, 638, 652
BseNI ACTGG 2 cut(s) 295, 331
BseRI GAGGAG 2 cut(s) 507, 619
BseXI GCAGC 3 cut(s) 55, 58, 307
Bsh1236I CGCG 1 cut(s) 649
BshFI GGCC 1 cut(s) 511
BsiHKAI GWGCWC 2 cut(s) 316, 611
BsiHKCI CYCGRG 2 cut(s) 601, 610
BsiSI CCGG 1 cut(s) 512
BslI CCNNNNNNNGG 2 cut(s) 161, 524
BsmAI GTCTC 5 cut(s) 229, 267, 568, 627, 641
BsnI GGCC 1 cut(s) 511
Bso31I GGTCTC 3 cut(s) 229, 568, 641
BsoBI CYCGRG 2 cut(s) 601, 610
Bsp1286I GDGCHC 2 cut(s) 316, 611
Bsp143I GATC 1 cut(s) 404
BspACI CCGC 3 cut(s) 420, 560, 647
BspANI GGCC 1 cut(s) 511
BspCNI CTCAG 4 cut(s) 319, 532, 637, 651
BspFNI CGCG 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 166, 516
BspQI GCTCTTC 1 cut(s) 237
BspTNI GGTCTC 3 cut(s) 229, 568, 641
BsrFI RCCGGY 1 cut(s) 511
BsrI ACTGG 2 cut(s) 295, 331
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 589
BssMI GATC 1 cut(s) 404
BssNI GRCGYC 1 cut(s) 581
Bst2UI CCWGG 2 cut(s) 105, 156
Bst4CI ACNGT 2 cut(s) 386, 445
Bst6I CTCTTC 4 cut(s) 173, 237, 484, 571
BstACI GRCGYC 1 cut(s) 581
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 4 cut(s) 306, 519, 624, 638
BstFNI CGCG 1 cut(s) 649
BstKTI GATC 1 cut(s) 407
BstMAI GTCTC 5 cut(s) 229, 267, 568, 627, 641
BstMBI GATC 1 cut(s) 404
BstMWI GCNNNNNNNGC 3 cut(s) 52, 211, 320
BstNI CCWGG 2 cut(s) 105, 156
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 2 cut(s) 103, 154
BstUI CGCG 1 cut(s) 649
BstV1I GCAGC 3 cut(s) 55, 58, 307
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 1 cut(s) 404
BstYI RGATCY 1 cut(s) 404
BsuRI GGCC 1 cut(s) 511
BtsIMutI CAGTG 3 cut(s) 314, 324, 646
Cac8I GCNNGC 1 cut(s) 513
Cfr10I RCCGGY 1 cut(s) 511
Cfr13I GGNCC 1 cut(s) 399
CsiI ACCWGGT 1 cut(s) 154
CviAII CATG 3 cut(s) 317, 331, 585
CviJI RGCY 9 cut(s) 88, 120, 205, 247, 511, 515, 572, 577, 609
CviKI_1 RGCY 9 cut(s) 88, 120, 205, 247, 511, 515, 572, 577, 609
DdeI CTNAG 4 cut(s) 306, 519, 624, 638
DpnI GATC 1 cut(s) 406
DpnII GATC 1 cut(s) 404
Eam1104I CTCTTC 4 cut(s) 173, 237, 484, 571
EarI CTCTTC 4 cut(s) 173, 237, 484, 571
Ecl136II GAGCTC 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 611
Eco31I GGTCTC 3 cut(s) 229, 568, 641
Eco47I GGWCC 1 cut(s) 399
Eco53kI GAGCTC 1 cut(s) 609
Eco57I CTGAAG 1 cut(s) 552
Eco88I CYCGRG 2 cut(s) 601, 610
EcoICRI GAGCTC 1 cut(s) 609
EcoRII CCWGG 2 cut(s) 103, 154
EcoT38I GRGCYC 1 cut(s) 611
FaeI CATG 3 cut(s) 320, 334, 588
FaiI YATR 4 cut(s) 318, 332, 336, 586
FalI AAGNNNNNCTT 4 cut(s) 7, 39, 152, 184
FatI CATG 3 cut(s) 316, 330, 584
FauI CCCGC 1 cut(s) 427
FblI GTMKAC 1 cut(s) 491
Fnu4HI GCNGC 4 cut(s) 44, 47, 321, 647
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 4 cut(s) 44, 47, 321, 647
FspBI CTAG 1 cut(s) 632
GluI GCNGC 4 cut(s) 44, 47, 321, 647
HaeIII GGCC 1 cut(s) 511
HapII CCGG 1 cut(s) 512
Hin1I GRCGYC 1 cut(s) 581
Hin1II CATG 3 cut(s) 320, 334, 588
HincII GTYRAC 2 cut(s) 448, 492
HindII GTYRAC 2 cut(s) 448, 492
HinfI GANTC 2 cut(s) 31, 599
HpaII CCGG 1 cut(s) 512
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 2 cut(s) 448, 492
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 4 cut(s) 226, 414, 468, 626
Hpy8I GTNNAC 2 cut(s) 448, 492
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 2 cut(s) 386, 445
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 2 cut(s) 338, 349
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 211, 320
HpyF3I CTNAG 4 cut(s) 306, 519, 624, 638
HpySE526I ACGT 1 cut(s) 581
Hsp92I GRCGYC 1 cut(s) 581
Hsp92II CATG 3 cut(s) 320, 334, 588
KroI GCCGGC 1 cut(s) 511
KroNI GCCGGC 1 cut(s) 513
Kzo9I GATC 1 cut(s) 404
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 520, 606
Lsp1109I GCAGC 3 cut(s) 55, 58, 307
LweI GCATC 2 cut(s) 311, 336
MabI ACCWGGT 1 cut(s) 154
MaeI CTAG 1 cut(s) 632
MaeII ACGT 1 cut(s) 581
MalI GATC 1 cut(s) 406
MboI GATC 1 cut(s) 404
MboII GAAGA 6 cut(s) 15, 190, 254, 471, 524, 558
MflI RGATCY 1 cut(s) 404
MhlI GDGCHC 2 cut(s) 316, 611
MluCI AATT 3 cut(s) 18, 94, 187
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 2 cut(s) 120, 377
MnlI CCTC 7 cut(s) 174, 190, 468, 528, 597, 599, 606
MroNI GCCGGC 1 cut(s) 511
MroXI GAANNNNTTC 2 cut(s) 22, 188
MseI TTAA 5 cut(s) 14, 71, 84, 249, 278
MslI CAYNNNNRTG 2 cut(s) 307, 329
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 105, 156
MvaI CCWGG 2 cut(s) 105, 156
MvnI CGCG 1 cut(s) 649
MwoI GCNNNNNNNGC 3 cut(s) 52, 211, 320
NaeI GCCGGC 1 cut(s) 513
NdeII GATC 1 cut(s) 404
NgoMIV GCCGGC 1 cut(s) 511
NlaIII CATG 3 cut(s) 320, 334, 588
NlaIV GGNNCC 2 cut(s) 166, 516
NmeAIII GCCGAG 2 cut(s) 553, 570
NspI RCATGY 1 cut(s) 334
PaeR7I CTCGAG 2 cut(s) 601, 610
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PdiI GCCGGC 1 cut(s) 513
PdmI GAANNNNTTC 2 cut(s) 22, 188
PfeI GAWTC 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 4 cut(s) 45, 48, 322, 648
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
Psp124BI GAGCTC 1 cut(s) 611
Psp6I CCWGG 2 cut(s) 103, 154
PspGI CCWGG 2 cut(s) 103, 154
PspN4I GGNNCC 2 cut(s) 166, 516
PspPI GGNCC 1 cut(s) 399
PspXI VCTCGAGB 2 cut(s) 601, 610
PsuI RGATCY 1 cut(s) 404
RseI CAYNNNNRTG 2 cut(s) 307, 329
SacI GAGCTC 1 cut(s) 611
SalI GTCGAC 1 cut(s) 490
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 5 cut(s) 14, 71, 84, 249, 278
SatI GCNGC 4 cut(s) 44, 47, 321, 647
Sau3AI GATC 1 cut(s) 404
Sau96I GGNCC 1 cut(s) 399
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 2 cut(s) 105, 156
SduI GDGCHC 2 cut(s) 316, 611
SetI ASST 9 cut(s) 157, 249, 367, 460, 574, 584, 611, 617, 637
SexAI ACCWGGT 1 cut(s) 154
SfaNI GCATC 2 cut(s) 311, 336
Sfr274I CTCGAG 2 cut(s) 601, 610
SinI GGWCC 1 cut(s) 399
SlaI CTCGAG 2 cut(s) 601, 610
SmiMI CAYNNNNRTG 2 cut(s) 307, 329
SmlI CTYRAG 5 cut(s) 76, 461, 505, 601, 610
SmoI CTYRAG 5 cut(s) 76, 461, 505, 601, 610
Sse9I AATT 3 cut(s) 18, 94, 187
SsiI CCGC 3 cut(s) 420, 560, 647
SspMI CTAG 1 cut(s) 632
SstI GAGCTC 1 cut(s) 611
StyD4I CCNGG 2 cut(s) 103, 154
TaaI ACNGT 2 cut(s) 386, 445
TaiI ACGT 1 cut(s) 584
TaqI TCGA 4 cut(s) 227, 491, 602, 611
TasI AATT 3 cut(s) 18, 94, 187
TauI GCSGC 1 cut(s) 649
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 5 cut(s) 14, 71, 84, 249, 278
Tru9I TTAA 5 cut(s) 14, 71, 84, 249, 278
TscAI CASTG 3 cut(s) 314, 331, 646
TseI GCWGC 3 cut(s) 43, 46, 320
TspDTI ATGAA 2 cut(s) 112, 255
TspRI CASTG 3 cut(s) 314, 331, 646
VpaK11BI GGWCC 1 cut(s) 399
XapI RAATTY 3 cut(s) 18, 94, 187
XceI RCATGY 1 cut(s) 334
XhoI CTCGAG 2 cut(s) 601, 610
XmiI GTMKAC 1 cut(s) 491
XmnI GAANNNNTTC 2 cut(s) 22, 188
XspI CTAG 1 cut(s) 632
ZraI GACGTC 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.