pycom15g08580

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009762
Physical Location & Seq
Reverse (-)
5450523 .. 5450959
437 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g08580.1

Sequence Viewer

Length: 279 bp
ATGGCCTCTCGCTGCAGATCTTTCTCGAAGCCGGCTTTCTCTCTTCTCAAATCTGCCGTCAAAAAACCGGCGTTCAAGGCCACTCCCTTGTCTTCTCTCCCCTCCACCCGCCCTTCAATCAATCTCTCAAGGCCGATTCCGCAGTTGGGTTGTCTACAATCTCTGCTTCCATTTCACACGGCGGTGTCTTCAGCTCGGCTCACGTCGTGCCTTGGCATTGACTCTAGGAGCTCGAGGTCGTTGTCTCAGGGTATGCTCGGTGCAAACCCAGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

9.67

Weight (kDa)

11.64

Isoelectric Point (pI)

73.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 154
AciI CCGC 3 cut(s) 109, 140, 182
AcuI CTGAAG 1 cut(s) 174
AdeI CACNNNGTG 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 2 cut(s) 76, 117
AjiI CACGTC 1 cut(s) 204
AjnI CCWGG 1 cut(s) 268
AleI CACNNNNGTG 1 cut(s) 182
AluBI AGCT 2 cut(s) 194, 231
AluI AGCT 2 cut(s) 194, 231
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 249
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 3 cut(s) 3, 78, 131
ApeKI GCWGC 1 cut(s) 12
ArsI GACNNNNNNTTYG 2 cut(s) 42, 74
AvaI CYCGRG 1 cut(s) 232
BanII GRGCYC 1 cut(s) 233
BbsI GAAGAC 2 cut(s) 84, 180
Bbv12I GWGCWC 1 cut(s) 233
BceAI ACGGC 2 cut(s) 41, 195
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 13
BglII AGATCT 1 cut(s) 17
BisI GCNGC 1 cut(s) 13
BlsI GCNGC 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 270
BmeT110I CYCGRG 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 270
BpiI GAAGAC 2 cut(s) 84, 180
BpuEI CTTGAG 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 211, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse118I RCCGGY 2 cut(s) 31, 67
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 211, 268
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 1 cut(s) 260
BshFI GGCC 3 cut(s) 5, 80, 133
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 2 cut(s) 32, 68
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 249
BsnI GGCC 3 cut(s) 5, 80, 133
BsoBI CYCGRG 1 cut(s) 232
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 1 cut(s) 17
BspACI CCGC 3 cut(s) 109, 140, 182
BspANI GGCC 3 cut(s) 5, 80, 133
BspCNI CTCAG 1 cut(s) 259
BspMAI CTGCAG 1 cut(s) 17
BsrFI RCCGGY 2 cut(s) 31, 67
BssAI RCCGGY 2 cut(s) 31, 67
BssECI CCNNGG 2 cut(s) 211, 268
BssMI GATC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 211
Bst2UI CCWGG 1 cut(s) 270
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 1 cut(s) 246
BstKTI GATC 1 cut(s) 20
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 1 cut(s) 17
BstMWI GCNNNNNNNGC 2 cut(s) 77, 139
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 13
BstV2I GAAGAC 2 cut(s) 84, 180
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 3 cut(s) 5, 80, 133
BtrI CACGTC 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 33
Cfr10I RCCGGY 2 cut(s) 31, 67
CviJI RGCY 8 cut(s) 5, 31, 35, 80, 133, 194, 199, 231
CviKI_1 RGCY 8 cut(s) 5, 31, 35, 80, 133, 194, 199, 231
DdeI CTNAG 1 cut(s) 246
DpnI GATC 1 cut(s) 19
DpnII GATC 1 cut(s) 17
DraIII CACNNNGTG 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 233
Eco53kI GAGCTC 1 cut(s) 231
Eco57I CTGAAG 1 cut(s) 174
Eco88I CYCGRG 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 211
FaiI YATR 1 cut(s) 254
FauI CCCGC 1 cut(s) 116
FblI GTMKAC 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 13
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 13
FspBI CTAG 1 cut(s) 225
GluI GCNGC 1 cut(s) 13
HaeIII GGCC 3 cut(s) 5, 80, 133
HapII CCGG 2 cut(s) 32, 68
HinfI GANTC 2 cut(s) 136, 221
HpaII CCGG 2 cut(s) 32, 68
Hpy166II GTNNAC 1 cut(s) 155
Hpy188III TCNNGA 1 cut(s) 25
Hpy8I GTNNAC 1 cut(s) 155
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 123
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 2 cut(s) 15, 263
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 139
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 1 cut(s) 203
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
Kzo9I GATC 1 cut(s) 17
LmnI GCTCC 1 cut(s) 228
LpnPI CCDG 4 cut(s) 45, 81, 233, 255
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 203
MalI GATC 1 cut(s) 19
MboI GATC 1 cut(s) 17
MboII GAAGA 3 cut(s) 35, 84, 180
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 233
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 3 cut(s) 16, 112, 228
MroNI GCCGGC 1 cut(s) 31
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 2 cut(s) 32, 68
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 77, 139
NaeI GCCGGC 1 cut(s) 33
NdeII GATC 1 cut(s) 17
NgoMIV GCCGGC 1 cut(s) 31
NmeAIII GCCGAG 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 182
PaeR7I CTCGAG 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 1 cut(s) 14
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspXI VCTCGAGB 1 cut(s) 232
PstI CTGCAG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 182
SacI GAGCTC 1 cut(s) 233
SatI GCNGC 1 cut(s) 13
Sau3AI GATC 1 cut(s) 17
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 233
SetI ASST 4 cut(s) 196, 206, 233, 239
SfcI CTRYAG 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 232
SlaI CTCGAG 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 127, 232
SmoI CTYRAG 2 cut(s) 127, 232
SsiI CCGC 3 cut(s) 109, 140, 182
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 211
TaiI ACGT 1 cut(s) 206
TaqI TCGA 2 cut(s) 26, 233
TfiI GAWTC 1 cut(s) 136
TseI GCWGC 1 cut(s) 12
XhoI CTCGAG 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 154
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.