MD08G1114400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010821
Physical Location & Seq
Reverse (-)
10277664 .. 10281418
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1114400.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGCTACTTCGGTGAAGGTGAAGAGCGGCGGCAACCTGCAGGATGGTTCTTCAAAGGGAAAGATTGGATATTCTGTTACCAAGAAGAAGATTGACAGCTCTTCAAGCAAGCAGGCAGCTGATTACAAGCAGAAATCTGTGCAAACTGTTACAAAAACTGAGGTAAAATCAAAACCAAATTCGGCATCAAAGACGATAACAAAGAAAACCACCACAAAAGTGAGAGAGAAGAAAGTGTACACTTTGGCTGGCCAGAAATTTGATCCTCCTGAAGAGAGAGAACCTCTGCGGATCTTCTACGAGTCATTGTCCAAACAGATACCAACAAGTGAAATGGCAGAATTCTGGATGATGGAGCATGGCCTATTGTCCTCTGAAAGATCCAGAAAGGCATATGAGAAGAAGCAGAGAAAGCAAAAGCAACTCCGTTTGGGAACTCCTCTTAAGTCTACACCAAAACCAGCAAGTAAACCTGAGAGTTCACAGAGGCCACAACAGCAGGCATCTAAGAATGGCGATGTAAAAGCCAAGAAGAGAGTTATCAAAGAGAGTGACGATGACGATGATTTCATTTTAAGTTCCAAGAGAAGGAGAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.42

Weight (kDa)

10.12

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010821)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11600 AT5G11600
fragaria_vesca FvH4_2g35630
malus_domestica MD08G1114400.v1.1 MD15G1093900.v1.1
prunus_persica Prupe.1G448700_v2.0.a1
pyrus_communis pycom08g09520 pycom15g08680
rosa_chinensis RchiOBHm_Chr6g0306181
rosa_laevigata RLG00000010817
rosa_multiflora Rmu_co8231179.1_g000001 Rmu_co8463041.1_g000001 Rmu_sc0003594.1_g000009
rosa_roxburghii Rroxscaffold_7G00161980
rosa_rugosa Rorug06G0347700
rosa_samantha Rh6AG460300 Rh6BG441100 Rh6CG474300 Rh6DG460600
rosa_wichuraiana Rw6G040090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 45
AccBSI CCGCTC 1 cut(s) 27
AccI GTMKAC 1 cut(s) 449
AciI CCGC 3 cut(s) 27, 30, 289
AclWI GGATC 3 cut(s) 257, 299, 375
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 3 cut(s) 178, 257, 341
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 588
AflII CTTAAG 1 cut(s) 443
AgsI TTSAA 2 cut(s) 54, 105
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 2 cut(s) 99, 119
AluI AGCT 2 cut(s) 99, 119
AlwI GGATC 3 cut(s) 257, 299, 375
AoxI GGCC 3 cut(s) 250, 361, 488
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 3 cut(s) 178, 257, 341
AsuHPI GGTGA 2 cut(s) 25, 31
BalI TGGCCA 1 cut(s) 252
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BbvI GCAGC 1 cut(s) 128
BccI CCATC 2 cut(s) 38, 346
BfmI CTRYAG 1 cut(s) 38
BfrI CTTAAG 1 cut(s) 443
BfuAI ACCTGC 1 cut(s) 45
BisI GCNGC 3 cut(s) 28, 31, 117
BlsI GCNGC 3 cut(s) 29, 32, 118
BmsI GCATC 2 cut(s) 194, 512
BplI GAGNNNNNCTC 2 cut(s) 268, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 588
BseGI GGATG 2 cut(s) 49, 354
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 2 cut(s) 150, 465
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 1 cut(s) 128
BshFI GGCC 3 cut(s) 252, 363, 490
BslI CCNNNNNNNGG 1 cut(s) 588
BsnI GGCC 3 cut(s) 252, 363, 490
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 3 cut(s) 262, 291, 380
BspACI CCGC 3 cut(s) 27, 30, 289
BspANI GGCC 3 cut(s) 252, 363, 490
BspCNI CTCAG 2 cut(s) 151, 466
BspMAI CTGCAG 1 cut(s) 42
BspMI ACCTGC 1 cut(s) 45
BspPI GGATC 3 cut(s) 257, 299, 375
BspQI GCTCTTC 2 cut(s) 17, 106
BspTI CTTAAG 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 27
BsrGI TGTACA 1 cut(s) 237
BssMI GATC 3 cut(s) 262, 291, 380
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 4 cut(s) 17, 106, 267, 527
BstAFI CTTAAG 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 237
BstC8I GCNNGC 4 cut(s) 110, 114, 250, 501
BstDEI CTNAG 3 cut(s) 159, 474, 507
BstF5I GGATG 2 cut(s) 49, 354
BstKTI GATC 3 cut(s) 265, 294, 383
BstMBI GATC 3 cut(s) 262, 291, 380
BstMWI GCNNNNNNNGC 3 cut(s) 105, 412, 496
BstSFI CTRYAG 1 cut(s) 38
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 2 cut(s) 291, 380
BstYI RGATCY 2 cut(s) 291, 380
BsuRI GGCC 3 cut(s) 252, 363, 490
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 2 cut(s) 49, 354
BveI ACCTGC 1 cut(s) 45
Cac8I GCNNGC 4 cut(s) 110, 114, 250, 501
Csp6I GTAC 1 cut(s) 238
CviAII CATG 1 cut(s) 359
CviJI RGCY 8 cut(s) 5, 99, 119, 248, 252, 363, 490, 527
CviKI_1 RGCY 8 cut(s) 5, 99, 119, 248, 252, 363, 490, 527
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 3 cut(s) 159, 474, 507
DpnI GATC 3 cut(s) 264, 293, 382
DpnII GATC 3 cut(s) 262, 291, 380
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 4 cut(s) 17, 106, 267, 527
EarI CTCTTC 4 cut(s) 17, 106, 267, 527
Eco57I CTGAAG 1 cut(s) 291
EcoRI GAATTC 1 cut(s) 341
FaeI CATG 1 cut(s) 362
FaiI YATR 3 cut(s) 360, 394, 396
FatI CATG 1 cut(s) 358
FauNDI CATATG 1 cut(s) 394
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 3 cut(s) 28, 31, 117
FokI GGATG 2 cut(s) 56, 361
Fsp4HI GCNGC 3 cut(s) 28, 31, 117
GluI GCNGC 3 cut(s) 28, 31, 117
HaeIII GGCC 3 cut(s) 252, 363, 490
Hin1II CATG 1 cut(s) 362
HinfI GANTC 1 cut(s) 302
HphI GGTGA 2 cut(s) 25, 31
Hpy166II GTNNAC 5 cut(s) 238, 240, 450, 470, 482
Hpy188I TCNGA 1 cut(s) 376
Hpy188III TCNNGA 3 cut(s) 269, 346, 384
Hpy8I GTNNAC 5 cut(s) 238, 240, 450, 470, 482
HpyAV CCTTC 2 cut(s) 10, 582
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 40, 142
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 412, 496
HpyF3I CTNAG 3 cut(s) 159, 474, 507
Hsp92II CATG 1 cut(s) 362
Kzo9I GATC 3 cut(s) 262, 291, 380
LguI GCTCTTC 2 cut(s) 17, 106
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 2 cut(s) 194, 512
MaeIII GTNAC 3 cut(s) 76, 148, 551
MalI GATC 3 cut(s) 264, 293, 382
MbiI CCGCTC 1 cut(s) 27
MboI GATC 3 cut(s) 262, 291, 380
MflI RGATCY 2 cut(s) 291, 380
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 3 cut(s) 178, 257, 341
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 6 cut(s) 154, 276, 294, 382, 450, 480
Mox20I TGGCCA 1 cut(s) 252
MscI TGGCCA 1 cut(s) 252
MseI TTAA 2 cut(s) 444, 575
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 119
MspCI CTTAAG 1 cut(s) 443
MwoI GCNNNNNNNGC 3 cut(s) 105, 412, 496
NdeI CATATG 1 cut(s) 394
NdeII GATC 3 cut(s) 262, 291, 380
NlaIII CATG 1 cut(s) 362
NmuCI GTSAC 1 cut(s) 551
OliI CACNNNNGTG 1 cut(s) 218
PciSI GCTCTTC 2 cut(s) 17, 106
PkrI GCNGC 3 cut(s) 29, 32, 118
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
PstI CTGCAG 1 cut(s) 42
PsuI RGATCY 2 cut(s) 291, 380
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 218
SapI GCTCTTC 2 cut(s) 17, 106
SaqAI TTAA 2 cut(s) 444, 575
SatI GCNGC 3 cut(s) 28, 31, 117
Sau3AI GATC 3 cut(s) 262, 291, 380
SbfI CCTGCAGG 1 cut(s) 42
SchI GAGTC 1 cut(s) 311
SdaI CCTGCAGG 1 cut(s) 42
SetI ASST 7 cut(s) 21, 39, 101, 121, 165, 286, 475
SfaNI GCATC 2 cut(s) 194, 512
SfcI CTRYAG 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse8387I CCTGCAGG 1 cut(s) 42
Sse9I AATT 3 cut(s) 178, 257, 341
SsiI CCGC 3 cut(s) 27, 30, 289
TaaI ACNGT 1 cut(s) 148
TasI AATT 3 cut(s) 178, 257, 341
TatI WGTACW 1 cut(s) 237
TauI GCSGC 2 cut(s) 30, 33
Tru1I TTAA 2 cut(s) 444, 575
Tru9I TTAA 2 cut(s) 444, 575
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 1 cut(s) 559
TspGWI ACGGA 1 cut(s) 416
Vha464I CTTAAG 1 cut(s) 443
XapI RAATTY 3 cut(s) 178, 257, 341
XmiI GTMKAC 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.