MD08G1121300.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009403
Physical Location & Seq
Forward (+)
11092671 .. 11095874
3204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1121300.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGACAGCAGAACCAAAGGAGGAATTGCCACCAGGATTCAGATTTCATCCTTCAGATGAAGAGCTTATCACCTTCTATCTCATGAACAAGATAAATGATGCCACTTTTACTGGAAGGGCGATTGCCGATGTTGATCTCAACAAATGCGAACCCTGGGAACTTCCTGCAAAAGCGAAAATGGGAGAAAAGGAGTGGTACTTCTTCAGCCTACGAGATCGGAAGTACCCAACAGGAGTGAGAACAAACCGAGCAACAAGTACAGGTTACTGGAAGACCACAGGGAAAGACAAAGAGATCTTCAACAGTGTCACATCTGAGCTGTTTGGGATGAAAAAGACCCTTGTTTTCTACAGAGGCCGAGCTCCTCGTGGTGAAAAATCCAACTGGGTCATGCATGAATATCGCATTCATTCTAAATCTGGCTTTAGAACATCAAAGGATGAATGGGTGGTTTGCCGAGTCTTCCAGAAGAGCGCAGGCATAAAAAAGTACCCAACAAACCAATCAAGAGCGGCCAATCCCTACAACCTAGAAATAGGACCAAGTATGATACCATCACCCCTAATGCAGCTAGGTGATCCAAACCACCATCAGTTCCCCTATGGCAGAAACTACATGACAAGTGCTGAGATGGCCGAGCTTTCAAGAGTCTTCAGAGGTGGCGGTGGTGGATCATCAAGTAGCAATATCAACCTACCAATCCAACCCCAATTCAATTACCCAATTGGTGGAGGAGGAGGTGGCGGTGGTGTTGGAGGAGGAGCAGGAGGGTTTACAATGTCTGGGTTGAACTTGAATCTTGGTGGGCCAGCATCACAGCCTATGTTGAGGACAATGCCACCACCACAGATGCATCATCATGGCCCTCCATCTATGAATCATCATCATCAAGTGCAAGATGTTACTATGATGAACGGTCCAGATCATCAAGCTGGATACGGAACAATAGACATGAACAACAGCAACAACGCAAACGGTGGGAGTGGAAACAGGTTTATGAACATGGATACTTGTGTTGATCTTGAGAACTACTGGCCTCCCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

349

Amino Acids

38.66

Weight (kDa)

8.95

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 10 - 136 4.9e-48 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 728
AccBSI CCGCTC 1 cut(s) 512
AciI CCGC 3 cut(s) 512, 663, 744
AclWI GGATC 2 cut(s) 572, 679
AcoI YGGCCR 2 cut(s) 513, 633
AcuI CTGAAG 3 cut(s) 36, 187, 637
AfaI GTAC 4 cut(s) 197, 224, 259, 491
AfiI CCNNNNNNNGG 1 cut(s) 728
AgsI TTSAA 5 cut(s) 301, 645, 715, 790, 796
AjnI CCWGG 2 cut(s) 31, 152
AluBI AGCT 6 cut(s) 64, 319, 362, 571, 640, 932
AluI AGCT 6 cut(s) 64, 319, 362, 571, 640, 932
Alw21I GWGCWC 1 cut(s) 364
AlwI GGATC 2 cut(s) 572, 679
AoxI GGCC 6 cut(s) 355, 513, 633, 806, 862, 1034
ApeKI GCWGC 1 cut(s) 568
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 4 cut(s) 539, 806, 863, 917
AsuHPI GGTGA 4 cut(s) 61, 383, 549, 587
AvaII GGWCC 2 cut(s) 539, 917
BaeI ACNNNNGTAYC 2 cut(s) 999, 1032
BanII GRGCYC 1 cut(s) 364
BauI CACGAG 1 cut(s) 366
BbsI GAAGAC 3 cut(s) 278, 454, 643
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 1 cut(s) 580
BccI CCATC 4 cut(s) 562, 597, 625, 877
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciT130I CCWGG 2 cut(s) 33, 154
BciVI GTATCC 2 cut(s) 929, 1000
BfaI CTAG 2 cut(s) 530, 572
BfmI CTRYAG 1 cut(s) 349
BfuI GTATCC 2 cut(s) 929, 1000
BglII AGATCT 1 cut(s) 294
BisI GCNGC 2 cut(s) 513, 569
BlsI GCNGC 2 cut(s) 514, 570
Bme1390I CCNGG 2 cut(s) 33, 154
Bme18I GGWCC 2 cut(s) 539, 917
BmgT120I GGNCC 4 cut(s) 539, 806, 863, 917
BmrFI CCNGG 2 cut(s) 33, 154
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 4 cut(s) 88, 821, 840, 862
BmuI ACTGGG 1 cut(s) 394
BpiI GAAGAC 3 cut(s) 278, 454, 643
BpuEI CTTGAG 1 cut(s) 1043
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 152, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 728
Bse1I ACTGG 4 cut(s) 115, 272, 389, 1037
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 2 cut(s) 33, 154
BseDI CCNNGG 2 cut(s) 152, 153
BseGI GGATG 3 cut(s) 46, 333, 445
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 728
BseMII CTCAG 2 cut(s) 306, 618
BseNI ACTGG 4 cut(s) 115, 272, 389, 1037
BseRI GAGGAG 5 cut(s) 354, 747, 750, 771, 774
BseXI GCAGC 1 cut(s) 580
BshFI GGCC 6 cut(s) 357, 515, 635, 808, 864, 1036
BsiHKAI GWGCWC 1 cut(s) 364
BslI CCNNNNNNNGG 1 cut(s) 728
BsmI GAATGC 1 cut(s) 405
BsnI GGCC 6 cut(s) 357, 515, 635, 808, 864, 1036
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
BspACI CCGC 3 cut(s) 512, 663, 744
BspANI GGCC 6 cut(s) 357, 515, 635, 808, 864, 1036
BspCNI CTCAG 2 cut(s) 307, 619
BspHI TCATGA 1 cut(s) 81
BspPI GGATC 2 cut(s) 572, 679
BspQI GCTCTTC 2 cut(s) 54, 464
BsrBI CCGCTC 1 cut(s) 512
BsrI ACTGG 4 cut(s) 115, 272, 389, 1037
BssECI CCNNGG 2 cut(s) 152, 153
BssMI GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
BssSI CACGAG 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 366
Bst2UI CCWGG 2 cut(s) 33, 154
Bst4CI ACNGT 3 cut(s) 305, 917, 977
Bst6I CTCTTC 2 cut(s) 54, 464
BstC8I GCNNGC 2 cut(s) 478, 810
BstDEI CTNAG 2 cut(s) 315, 627
BstF5I GGATG 3 cut(s) 46, 333, 445
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 7 cut(s) 136, 217, 297, 580, 674, 925, 1021
BstMBI GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 2 cut(s) 33, 154
BstSCI CCNGG 2 cut(s) 31, 152
BstSFI CTRYAG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 3 cut(s) 278, 454, 643
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuI GTATCC 2 cut(s) 929, 1000
BsuRI GGCC 6 cut(s) 357, 515, 635, 808, 864, 1036
BtsCI GGATG 3 cut(s) 46, 333, 445
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 478, 810
CciI TCATGA 1 cut(s) 81
CfoI GCGC 1 cut(s) 476
Cfr13I GGNCC 4 cut(s) 539, 806, 863, 917
Csp6I GTAC 4 cut(s) 196, 223, 258, 490
CviAII CATG 7 cut(s) 82, 391, 395, 616, 860, 952, 1003
CviQI GTAC 4 cut(s) 196, 223, 258, 490
DdeI CTNAG 2 cut(s) 315, 627
DpnI GATC 7 cut(s) 135, 216, 296, 579, 673, 924, 1020
DpnII GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
EaeI YGGCCR 2 cut(s) 513, 633
Eam1104I CTCTTC 2 cut(s) 54, 464
EarI CTCTTC 2 cut(s) 54, 464
Ecl136II GAGCTC 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 539, 917
Eco53kI GAGCTC 1 cut(s) 362
Eco57I CTGAAG 3 cut(s) 36, 187, 637
EcoICRI GAGCTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 31, 152
EcoT22I ATGCAT 2 cut(s) 396, 855
EcoT38I GRGCYC 1 cut(s) 364
FaeI CATG 7 cut(s) 85, 394, 398, 619, 863, 955, 1006
FatI CATG 7 cut(s) 81, 390, 394, 615, 859, 951, 1002
Fnu4HI GCNGC 2 cut(s) 513, 569
FokI GGATG 3 cut(s) 33, 340, 452
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 2 cut(s) 513, 569
FspBI CTAG 2 cut(s) 530, 572
GlaI GCGC 1 cut(s) 475
GluI GCNGC 2 cut(s) 513, 569
HaeIII GGCC 6 cut(s) 357, 515, 635, 808, 864, 1036
HhaI GCGC 1 cut(s) 476
Hin1II CATG 7 cut(s) 85, 394, 398, 619, 863, 955, 1006
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HinfI GANTC 5 cut(s) 36, 459, 648, 796, 877
HphI GGTGA 4 cut(s) 61, 383, 549, 587
Hpy166II GTNNAC 1 cut(s) 774
Hpy188I TCNGA 5 cut(s) 41, 55, 219, 316, 656
Hpy188III TCNNGA 6 cut(s) 82, 466, 507, 645, 920, 1022
Hpy8I GTNNAC 1 cut(s) 774
HpyAV CCTTC 3 cut(s) 60, 82, 108
HpyCH4III ACNGT 3 cut(s) 305, 917, 977
HpyCH4V TGCA 5 cut(s) 167, 394, 568, 853, 895
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 2 cut(s) 315, 627
Hsp92II CATG 7 cut(s) 85, 394, 398, 619, 863, 955, 1006
HspAI GCGC 1 cut(s) 474
Kzo9I GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
LguI GCTCTTC 2 cut(s) 54, 464
LmnI GCTCC 2 cut(s) 367, 761
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 4 cut(s) 88, 821, 840, 862
MaeI CTAG 2 cut(s) 530, 572
MaeIII GTNAC 3 cut(s) 263, 307, 901
MalI GATC 7 cut(s) 135, 216, 296, 579, 673, 924, 1020
MbiI CCGCTC 1 cut(s) 512
MboI GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
MboII GAAGA 7 cut(s) 71, 193, 283, 289, 454, 481, 643
MfeI CAATTG 1 cut(s) 723
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 4 cut(s) 23, 710, 715, 723
MlyI GAGTC 2 cut(s) 468, 657
MmeI TCCRAC 3 cut(s) 405, 727, 733
Mph1103I ATGCAT 2 cut(s) 396, 855
MslI CAYNNNNRTG 1 cut(s) 858
MspR9I CCNGG 2 cut(s) 33, 154
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 1 cut(s) 405
MvaI CCWGG 2 cut(s) 33, 154
MwoI GCNNNNNNNGC 1 cut(s) 632
NdeII GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
NlaIII CATG 7 cut(s) 85, 394, 398, 619, 863, 955, 1006
NmeAIII GCCGAG 3 cut(s) 383, 482, 661
NmuCI GTSAC 1 cut(s) 307
NsiI ATGCAT 2 cut(s) 396, 855
PagI TCATGA 1 cut(s) 81
PasI CCCWGGG 1 cut(s) 153
PciSI GCTCTTC 2 cut(s) 54, 464
PctI GAATGC 1 cut(s) 405
PfeI GAWTC 3 cut(s) 36, 796, 877
PflMI CCANNNNNTGG 1 cut(s) 728
PkrI GCNGC 2 cut(s) 514, 570
PleI GAGTC 2 cut(s) 467, 656
PpsI GAGTC 2 cut(s) 467, 656
Psp124BI GAGCTC 1 cut(s) 364
Psp6I CCWGG 2 cut(s) 31, 152
PspGI CCWGG 2 cut(s) 31, 152
PspPI GGNCC 4 cut(s) 539, 806, 863, 917
PsuI RGATCY 1 cut(s) 294
RsaI GTAC 4 cut(s) 197, 224, 259, 491
RsaNI GTAC 4 cut(s) 196, 223, 258, 490
RseI CAYNNNNRTG 1 cut(s) 858
SacI GAGCTC 1 cut(s) 364
SapI GCTCTTC 2 cut(s) 54, 464
SatI GCNGC 2 cut(s) 513, 569
Sau3AI GATC 7 cut(s) 133, 214, 294, 577, 671, 922, 1018
Sau96I GGNCC 4 cut(s) 539, 806, 863, 917
SchI GAGTC 2 cut(s) 468, 657
ScrFI CCNGG 2 cut(s) 33, 154
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 4 cut(s) 88, 821, 840, 862
SfcI CTRYAG 1 cut(s) 349
SinI GGWCC 2 cut(s) 539, 917
SmiMI CAYNNNNRTG 1 cut(s) 858
SmlI CTYRAG 1 cut(s) 1022
SmoI CTYRAG 1 cut(s) 1022
Sse9I AATT 4 cut(s) 23, 710, 715, 723
SsiI CCGC 3 cut(s) 512, 663, 744
SspMI CTAG 2 cut(s) 530, 572
SstI GAGCTC 1 cut(s) 364
StyD4I CCNGG 2 cut(s) 31, 152
TaaI ACNGT 3 cut(s) 305, 917, 977
TasI AATT 4 cut(s) 23, 710, 715, 723
TatI WGTACW 1 cut(s) 257
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 3 cut(s) 36, 796, 877
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 1 cut(s) 568
Tsp45I GTSAC 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 728
VpaK11BI GGWCC 2 cut(s) 539, 917
XspI CTAG 2 cut(s) 530, 572
Zsp2I ATGCAT 2 cut(s) 396, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.