MD15G1100100.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009403
Physical Location & Seq
Forward (+)
6952781 .. 6954972
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1100100.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGACAGCAGAGCCAAAGGAGGAATTGCCACCAGGGTTCAGATTTCATCCTACGGATGAAGAACTTATCACCTTCTATCTCATGAACAAGATAAATGATGCCACTTTTACTGGAAGGGCGATCGCCGATGTTGATCTCAACAAATGCGAACCCTGGGAACTTCCTGCAAAAGCGAAAATGGGAGAAAAGGAGTGGTACTTTTTCAGCCTACGAGATCGAAAGTACCCAACGGGAGTGAGAACAAACCGAGCAACAAACATAGGTTACTGGAAGACCACAGGAAAAGACAAGGAGATCTTCAACAGTGTCACATCTGAGCTAGCTGGGATGAAAAAGACCCTTGTTTTCTACAGAGGCCGAGCTCCTCGTGGCGAAAAATCCAACTGGGTCATGCACGAATATCGGATTCATTCTAAATCCGGCTTTAGAACATCCAAGGATGAGTGGGTGATTTGTCGAGTCTTCCAAAAGAGTGCAGGTATAAAAAAGTACCCAACGAACCAATCAAGAGCGGCCAATCCCTACAACCTAGAAATAGGTCCAAGTATGGTACCATCACCCCTAATGCAGCTAGGTGATCCAAACCACCATCAGTTCCCCTATGGCAGAAATTACATGACTAGCGCTGAGATGGCAGAGATTTCAAGAGTCTTAAGAGGCGGCGGATCATCAAGTAGCACTGTTAACCTACCAATCCAACCCCAGTTCAATTACCCAATTGGTGGAGGAGGTGGTGGTGTTGGAGGAGGAGGAGGGTTTACAATATCTGGGTTGAACTTGAATCTTGGTGGCCCAGCATCACAGCCTATGTTGAGGACAATGCCACCACCACATCTGCATCATCAAGGCCCTCCATCTATGAATCATCATCCTCAAGTGCAAGATGTTACTAATACAGCCATGATGAACGGTCCAGATCATCAAGGTGGATATGGAACAATAGACATGAACAACAACAACGCAAATGGTGGGAGTGGAAACAGGTTTATGAACATGGAGCCTTGTGTTGATCTTGATAACTACTGGCCTCCCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

346

Amino Acids

38.38

Weight (kDa)

8.95

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 10 - 136 6.7e-48 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 467
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 1 cut(s) 550
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 512
AciI CCGC 3 cut(s) 512, 660, 663
AclWI GGATC 2 cut(s) 572, 673
AcoI YGGCCR 1 cut(s) 513
AfaI GTAC 4 cut(s) 197, 224, 491, 552
AfeI AGCGCT 1 cut(s) 625
AfiI CCNNNNNNNGG 1 cut(s) 722
AflII CTTAAG 1 cut(s) 652
AgsI TTSAA 5 cut(s) 301, 645, 709, 775, 781
AjnI CCWGG 2 cut(s) 31, 152
AluBI AGCT 4 cut(s) 319, 323, 362, 571
AluI AGCT 4 cut(s) 319, 323, 362, 571
Alw21I GWGCWC 1 cut(s) 364
AlwI GGATC 2 cut(s) 572, 673
Aor51HI AGCGCT 1 cut(s) 625
AoxI GGCC 5 cut(s) 355, 513, 790, 847, 1025
ApeKI GCWGC 1 cut(s) 568
AsiSI GCGATCGC 1 cut(s) 123
Asp718I GGTACC 1 cut(s) 550
AspLEI GCGC 1 cut(s) 626
AspS9I GGNCC 4 cut(s) 539, 791, 848, 911
AsuHPI GGTGA 4 cut(s) 61, 460, 549, 587
AsuNHI GCTAGC 1 cut(s) 319
AvaII GGWCC 2 cut(s) 539, 911
BanI GGYRCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 364
BauI CACGAG 1 cut(s) 366
BbsI GAAGAC 2 cut(s) 278, 454
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 1 cut(s) 580
BccI CCATC 4 cut(s) 562, 597, 625, 862
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciT130I CCWGG 2 cut(s) 33, 154
BfaI CTAG 4 cut(s) 320, 530, 572, 621
BfmI CTRYAG 1 cut(s) 349
BfoI RGCGCY 1 cut(s) 627
BfrI CTTAAG 1 cut(s) 652
BfuAI ACCTGC 1 cut(s) 467
BglII AGATCT 1 cut(s) 294
BisI GCNGC 3 cut(s) 513, 569, 661
BlsI GCNGC 3 cut(s) 514, 570, 662
Bme1390I CCNGG 2 cut(s) 33, 154
Bme18I GGWCC 2 cut(s) 539, 911
BmgT120I GGNCC 4 cut(s) 539, 791, 848, 911
BmiI GGNNCC 2 cut(s) 552, 999
BmrFI CCNGG 2 cut(s) 33, 154
BmrI ACTGGG 2 cut(s) 394, 697
BmsI GCATC 3 cut(s) 88, 806, 847
BmtI GCTAGC 1 cut(s) 323
BmuI ACTGGG 2 cut(s) 394, 697
BpiI GAAGAC 2 cut(s) 278, 454
BpuEI CTTGAG 1 cut(s) 858
BsaBI GATNNNNATC 1 cut(s) 132
BsaJI CCNNGG 4 cut(s) 32, 152, 153, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse1I ACTGG 5 cut(s) 115, 272, 389, 703, 1028
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 2 cut(s) 33, 154
BseDI CCNNGG 4 cut(s) 32, 152, 153, 435
BseGI GGATG 6 cut(s) 46, 61, 333, 431, 445, 868
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMII CTCAG 2 cut(s) 306, 618
BseNI ACTGG 5 cut(s) 115, 272, 389, 703, 1028
BseRI GAGGAG 5 cut(s) 354, 741, 759, 762, 765
BseXI GCAGC 1 cut(s) 580
BseYI CCCAGC 2 cut(s) 323, 793
BsgI GTGCAG 1 cut(s) 495
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 5 cut(s) 357, 515, 792, 849, 1027
BshNI GGYRCC 1 cut(s) 550
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 420
BslI CCNNNNNNNGG 1 cut(s) 722
BsnI GGCC 5 cut(s) 357, 515, 792, 849, 1027
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
BspACI CCGC 3 cut(s) 512, 660, 663
BspANI GGCC 5 cut(s) 357, 515, 792, 849, 1027
BspCNI CTCAG 2 cut(s) 307, 619
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 552, 999
BspMI ACCTGC 1 cut(s) 467
BspOI GCTAGC 1 cut(s) 323
BspPI GGATC 2 cut(s) 572, 673
BspT107I GGYRCC 1 cut(s) 550
BspTI CTTAAG 1 cut(s) 652
BsrBI CCGCTC 1 cut(s) 512
BsrI ACTGG 5 cut(s) 115, 272, 389, 703, 1028
BssECI CCNNGG 4 cut(s) 32, 152, 153, 435
BssMI GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
BssSI CACGAG 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 366
Bst2UI CCWGG 2 cut(s) 33, 154
Bst4CI ACNGT 3 cut(s) 305, 682, 911
BstAFI CTTAAG 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 315, 627
BstF5I GGATG 6 cut(s) 46, 61, 333, 431, 445, 868
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 1 cut(s) 626
BstKTI GATC 8 cut(s) 123, 136, 217, 297, 580, 668, 919, 1012
BstMBI GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 2 cut(s) 33, 154
BstSCI CCNGG 2 cut(s) 31, 152
BstSFI CTRYAG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 2 cut(s) 278, 454
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 5 cut(s) 357, 515, 792, 849, 1027
BtsCI GGATG 6 cut(s) 46, 61, 333, 431, 445, 868
BtsIMutI CAGTG 2 cut(s) 310, 678
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 1 cut(s) 321
CciI TCATGA 1 cut(s) 81
CfoI GCGC 1 cut(s) 626
Cfr13I GGNCC 4 cut(s) 539, 791, 848, 911
Csp6I GTAC 4 cut(s) 196, 223, 490, 551
CviAII CATG 6 cut(s) 82, 391, 616, 901, 946, 994
CviQI GTAC 4 cut(s) 196, 223, 490, 551
DdeI CTNAG 2 cut(s) 315, 627
DpnI GATC 8 cut(s) 122, 135, 216, 296, 579, 667, 918, 1011
DpnII GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
EaeI YGGCCR 1 cut(s) 513
EciI GGCGGA 1 cut(s) 678
Ecl136II GAGCTC 1 cut(s) 362
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 539, 911
Eco47III AGCGCT 1 cut(s) 625
Eco53kI GAGCTC 1 cut(s) 362
EcoICRI GAGCTC 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 848
EcoRII CCWGG 2 cut(s) 31, 152
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 6 cut(s) 85, 394, 619, 904, 949, 997
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FatI CATG 6 cut(s) 81, 390, 615, 900, 945, 993
Fnu4HI GCNGC 3 cut(s) 513, 569, 661
FokI GGATG 6 cut(s) 33, 68, 340, 418, 452, 855
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 3 cut(s) 513, 569, 661
FspBI CTAG 4 cut(s) 320, 530, 572, 621
GlaI GCGC 1 cut(s) 625
GluI GCNGC 3 cut(s) 513, 569, 661
GsaI CCCAGC 2 cut(s) 327, 797
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 5 cut(s) 357, 515, 792, 849, 1027
HapII CCGG 1 cut(s) 420
HhaI GCGC 1 cut(s) 626
Hin1II CATG 6 cut(s) 85, 394, 619, 904, 949, 997
Hin6I GCGC 1 cut(s) 624
HinP1I GCGC 1 cut(s) 624
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HinfI GANTC 5 cut(s) 406, 459, 648, 781, 862
HpaI GTTAAC 1 cut(s) 685
HpaII CCGG 1 cut(s) 420
HphI GGTGA 4 cut(s) 61, 460, 549, 587
Hpy166II GTNNAC 2 cut(s) 685, 759
Hpy188I TCNGA 3 cut(s) 41, 316, 405
Hpy188III TCNNGA 5 cut(s) 82, 507, 645, 914, 1013
Hpy8I GTNNAC 2 cut(s) 685, 759
HpyAV CCTTC 2 cut(s) 82, 108
HpyCH4III ACNGT 3 cut(s) 305, 682, 911
HpyCH4V TGCA 6 cut(s) 167, 394, 476, 568, 838, 880
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 2 cut(s) 315, 627
Hsp92II CATG 6 cut(s) 85, 394, 619, 904, 949, 997
HspAI GCGC 1 cut(s) 624
KpnI GGTACC 1 cut(s) 554
KspAI GTTAAC 1 cut(s) 685
Kzo9I GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
LmnI GCTCC 2 cut(s) 367, 997
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 3 cut(s) 88, 806, 847
MaeI CTAG 4 cut(s) 320, 530, 572, 621
MaeIII GTNAC 3 cut(s) 263, 307, 886
MalI GATC 8 cut(s) 122, 135, 216, 296, 579, 667, 918, 1011
MbiI CCGCTC 1 cut(s) 512
MboI GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
MboII GAAGA 4 cut(s) 71, 283, 289, 454
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 4 cut(s) 23, 610, 709, 717
MlyI GAGTC 2 cut(s) 468, 657
MmeI TCCRAC 3 cut(s) 405, 721, 721
MseI TTAA 2 cut(s) 653, 684
MslI CAYNNNNRTG 1 cut(s) 924
MspCI CTTAAG 1 cut(s) 652
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 2 cut(s) 33, 154
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 2 cut(s) 33, 154
MwoI GCNNNNNNNGC 1 cut(s) 632
NdeII GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
NheI GCTAGC 1 cut(s) 319
NlaIII CATG 6 cut(s) 85, 394, 619, 904, 949, 997
NlaIV GGNNCC 2 cut(s) 552, 999
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 307
PagI TCATGA 1 cut(s) 81
PasI CCCWGGG 1 cut(s) 153
PfeI GAWTC 3 cut(s) 406, 781, 862
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 3 cut(s) 514, 570, 662
Ple19I CGATCG 1 cut(s) 123
PleI GAGTC 2 cut(s) 467, 656
PpsI GAGTC 2 cut(s) 467, 656
Psp124BI GAGCTC 1 cut(s) 364
Psp6I CCWGG 2 cut(s) 31, 152
PspFI CCCAGC 2 cut(s) 323, 793
PspGI CCWGG 2 cut(s) 31, 152
PspN4I GGNNCC 2 cut(s) 552, 999
PspPI GGNCC 4 cut(s) 539, 791, 848, 911
PsuI RGATCY 1 cut(s) 294
PvuI CGATCG 1 cut(s) 123
RgaI GCGATCGC 1 cut(s) 123
RsaI GTAC 4 cut(s) 197, 224, 491, 552
RsaNI GTAC 4 cut(s) 196, 223, 490, 551
RseI CAYNNNNRTG 1 cut(s) 924
SacI GAGCTC 1 cut(s) 364
SaqAI TTAA 2 cut(s) 653, 684
SatI GCNGC 3 cut(s) 513, 569, 661
Sau3AI GATC 8 cut(s) 120, 133, 214, 294, 577, 665, 916, 1009
Sau96I GGNCC 4 cut(s) 539, 791, 848, 911
SchI GAGTC 2 cut(s) 468, 657
ScrFI CCNGG 2 cut(s) 33, 154
SduI GDGCHC 1 cut(s) 364
SfaAI GCGATCGC 1 cut(s) 123
SfaNI GCATC 3 cut(s) 88, 806, 847
SfcI CTRYAG 1 cut(s) 349
SgfI GCGATCGC 1 cut(s) 123
SinI GGWCC 2 cut(s) 539, 911
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 2 cut(s) 652, 873
SmoI CTYRAG 2 cut(s) 652, 873
Sse9I AATT 4 cut(s) 23, 610, 709, 717
SsiI CCGC 3 cut(s) 512, 660, 663
SspMI CTAG 4 cut(s) 320, 530, 572, 621
SstI GAGCTC 1 cut(s) 364
StyD4I CCNGG 2 cut(s) 31, 152
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 3 cut(s) 305, 682, 911
TaqI TCGA 2 cut(s) 217, 457
TasI AATT 4 cut(s) 23, 610, 709, 717
TauI GCSGC 2 cut(s) 515, 663
TfiI GAWTC 3 cut(s) 406, 781, 862
Tru1I TTAA 2 cut(s) 653, 684
Tru9I TTAA 2 cut(s) 653, 684
TscAI CASTG 2 cut(s) 310, 685
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 1 cut(s) 568
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 9 cut(s) 35, 72, 98, 344, 398, 875, 920, 962, 1004
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 2 cut(s) 310, 685
Van91I CCANNNNNTGG 1 cut(s) 722
Vha464I CTTAAG 1 cut(s) 652
VpaK11BI GGWCC 2 cut(s) 539, 911
XspI CTAG 4 cut(s) 320, 530, 572, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.