MD08G1123200.v1.1

Calmodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016772
Physical Location & Seq
Forward (+)
11382191 .. 11382853
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1123200.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGCAAACACTATAAAAACTGTGGACACTTTTGAAGGAAATCACACACATTTCATATTTCCATATTTGCAAACTTCATTAGGGTTTCTGTGCAGTGGCATTATGGGAATTACAGGAGTTTGCTCTAGGTTACTTGGTGACCTGGTTCAAGCCATTGGAGGAGTCCCATCCAGTTCAGCAAGCAAAGGAGCTCACATTGATCAGTGTGATCAGTACTCCAATTGCAAACCACAAAGCATTGCAACCAAAGTTGATGAGTCCATGGCAAGGGCACTGATCACAGTGTTTGGGATGGAAACAAATGGAAGAATCAAGAAGGAAAAAGTGAGGCGCGTCGTTGAAAATCTTGGACTAATACGTAATGAGGAAGACCAGACGGGCTTCGATTTACCTGGGGATGATCATGAAGTGCCAGTGGAGGAGGTCCTTGGTGGATTGGAGGATAACGAGTCCGAGCGCAGCGAGCTCCTGCGTGAAGCATTCAAGATTTTCGACGAGGATGGCAACGGGTTCATAGAGGCTGTGGAGTTGAAAAGGGTTTTAGAGTGCTTGGGGTTGGGTAGTGGGTGGGAGATGGATCAGGTTGAGAAGATGGTCAAGGTTGTGGATTTGAATTTGGATGGTAAGGTTGATTTTGGAGAGTTTGAGTTAATGATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

221

Amino Acids

24.2

Weight (kDa)

4.55

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 157 - 219 3e-13 EF-hand domain pair
EF-hand_1 PF00036 157 - 182 3.3e-06 EF hand domain
EF-hand_6 PF13405 157 - 185 5.2e-08 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016772)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36371
malus_domestica MD08G1123200.v1.1 MD15G1102200.v1.1
prunus_persica Prupe.1G455200_v2.0.a1
pyrus_communis pycom15g09340
rosa_laevigata RLG00000010883
rosa_multiflora Rmu_sc0001809.1_g000003
rosa_roxburghii Rroxscaffold_7G00162730
rosa_rugosa Rorug06G0340900
rosa_samantha Rh6AG453100 Rh6BG448700 Rh6CG466800 Rh6DG453500
rosa_wichuraiana Rw6G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 333
AclWI GGATC 1 cut(s) 585
AcsI RAATTY 1 cut(s) 613
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 6 cut(s) 35, 149, 341, 484, 532, 613
AjnI CCWGG 2 cut(s) 141, 391
AluBI AGCT 2 cut(s) 191, 466
AluI AGCT 2 cut(s) 191, 466
Alw21I GWGCWC 2 cut(s) 193, 468
AlwI GGATC 1 cut(s) 585
ApeKI GCWGC 1 cut(s) 459
ApoI RAATTY 1 cut(s) 613
AspLEI GCGC 2 cut(s) 333, 459
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 149
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 274
BanII GRGCYC 2 cut(s) 193, 468
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 2 cut(s) 193, 468
BbvI GCAGC 1 cut(s) 471
BccI CCATC 7 cut(s) 175, 286, 494, 568, 586, 614, 649
BciT130I CCWGG 2 cut(s) 143, 393
BclI TGATCA 4 cut(s) 199, 208, 276, 400
BfaI CTAG 1 cut(s) 126
BisI GCNGC 1 cut(s) 460
BlsI GCNGC 1 cut(s) 461
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 2 cut(s) 143, 393
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmrFI CCNGG 2 cut(s) 143, 393
BpiI GAAGAC 1 cut(s) 375
BsaAI YACGTR 1 cut(s) 359
BsaJI CCNNGG 3 cut(s) 261, 392, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse1I ACTGG 2 cut(s) 171, 413
Bse3DI GCAATG 1 cut(s) 237
BseBI CCWGG 2 cut(s) 143, 393
BseDI CCNNGG 3 cut(s) 261, 392, 427
BseGI GGATG 5 cut(s) 167, 297, 403, 505, 625
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMI GCAATG 1 cut(s) 237
BseNI ACTGG 2 cut(s) 171, 413
BseRI GAGGAG 2 cut(s) 174, 434
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 1 cut(s) 471
BsgI GTGCAG 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 333
BsiHKAI GWGCWC 2 cut(s) 193, 468
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 149
BsmI GAATGC 1 cut(s) 479
Bsp1286I GDGCHC 3 cut(s) 193, 274, 468
Bsp143I GATC 5 cut(s) 199, 208, 276, 400, 577
Bsp19I CCATGG 1 cut(s) 261
BspFNI CGCG 1 cut(s) 333
BspHI TCATGA 1 cut(s) 403
BspPI GGATC 1 cut(s) 585
BsrDI GCAATG 1 cut(s) 237
BsrI ACTGG 2 cut(s) 171, 413
BssECI CCNNGG 3 cut(s) 261, 392, 427
BssMI GATC 5 cut(s) 199, 208, 276, 400, 577
BssT1I CCWWGG 2 cut(s) 261, 427
Bst2UI CCWGG 2 cut(s) 143, 393
Bst4CI ACNGT 2 cut(s) 22, 283
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 181, 464
BstDSI CCRYGG 1 cut(s) 261
BstEII GGTNACC 1 cut(s) 137
BstF5I GGATG 5 cut(s) 167, 297, 403, 505, 625
BstFNI CGCG 1 cut(s) 333
BstHHI GCGC 2 cut(s) 333, 459
BstKTI GATC 5 cut(s) 202, 211, 279, 403, 580
BstMBI GATC 5 cut(s) 199, 208, 276, 400, 577
BstMWI GCNNNNNNNGC 1 cut(s) 463
BstNI CCWGG 2 cut(s) 143, 393
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 2 cut(s) 141, 391
BstSLI GKGCMC 1 cut(s) 274
BstSNI TACGTA 1 cut(s) 359
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 1 cut(s) 471
BstV2I GAAGAC 1 cut(s) 375
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 5 cut(s) 167, 297, 403, 505, 625
BtsI GCAGTG 1 cut(s) 100
BtsIMutI CAGTG 5 cut(s) 100, 209, 272, 288, 420
Cac8I GCNNGC 2 cut(s) 181, 464
CciI TCATGA 1 cut(s) 403
CfoI GCGC 2 cut(s) 333, 459
Cfr13I GGNCC 1 cut(s) 424
CseI GACGC 1 cut(s) 322
CsiI ACCWGGT 1 cut(s) 141
Csp6I GTAC 1 cut(s) 214
CviAII CATG 2 cut(s) 262, 404
CviJI RGCY 5 cut(s) 152, 191, 381, 466, 521
CviKI_1 RGCY 5 cut(s) 152, 191, 381, 466, 521
CviQI GTAC 1 cut(s) 214
DpnI GATC 5 cut(s) 201, 210, 278, 402, 579
DpnII GATC 5 cut(s) 199, 208, 276, 400, 577
Ecl136II GAGCTC 2 cut(s) 191, 466
Eco105I TACGTA 1 cut(s) 359
Eco130I CCWWGG 2 cut(s) 261, 427
Eco24I GRGCYC 2 cut(s) 193, 468
Eco47I GGWCC 1 cut(s) 424
Eco53kI GAGCTC 2 cut(s) 191, 466
Eco91I GGTNACC 1 cut(s) 137
EcoICRI GAGCTC 2 cut(s) 191, 466
EcoO109I RGGNCCY 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 137
EcoRII CCWGG 2 cut(s) 141, 391
EcoT14I CCWWGG 2 cut(s) 261, 427
EcoT38I GRGCYC 2 cut(s) 193, 468
ErhI CCWWGG 2 cut(s) 261, 427
FaeI CATG 2 cut(s) 265, 407
FaiI YATR 7 cut(s) 14, 56, 64, 104, 263, 405, 515
FaqI GGGAC 1 cut(s) 149
FatI CATG 2 cut(s) 261, 403
FbaI TGATCA 4 cut(s) 199, 208, 276, 400
Fnu4HI GCNGC 1 cut(s) 460
FokI GGATG 5 cut(s) 154, 304, 410, 512, 632
FriOI GRGCYC 2 cut(s) 193, 468
Fsp4HI GCNGC 1 cut(s) 460
FspBI CTAG 1 cut(s) 126
GlaI GCGC 2 cut(s) 332, 458
GluI GCNGC 1 cut(s) 460
HgaI GACGC 1 cut(s) 322
HhaI GCGC 2 cut(s) 333, 459
Hin1II CATG 2 cut(s) 265, 407
Hin6I GCGC 2 cut(s) 331, 457
HinP1I GCGC 2 cut(s) 331, 457
HinfI GANTC 4 cut(s) 162, 257, 309, 449
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 3 cut(s) 313, 404, 484
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 2 cut(s) 338, 497
HpyAV CCTTC 2 cut(s) 29, 310
HpyCH4III ACNGT 2 cut(s) 22, 283
HpyCH4IV ACGT 1 cut(s) 358
HpyCH4V TGCA 4 cut(s) 70, 93, 225, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 463
HpySE526I ACGT 1 cut(s) 358
Hsp92II CATG 2 cut(s) 265, 407
HspAI GCGC 2 cut(s) 331, 457
Ksp22I TGATCA 4 cut(s) 199, 208, 276, 400
Kzo9I GATC 5 cut(s) 199, 208, 276, 400, 577
LmnI GCTCC 2 cut(s) 188, 471
Lsp1109I GCAGC 1 cut(s) 471
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 358
MaeIII GTNAC 2 cut(s) 129, 137
MalI GATC 5 cut(s) 201, 210, 278, 402, 579
MboI GATC 5 cut(s) 199, 208, 276, 400, 577
MboII GAAGA 3 cut(s) 318, 380, 601
MfeI CAATTG 1 cut(s) 220
MhlI GDGCHC 3 cut(s) 193, 274, 468
MluCI AATT 3 cut(s) 108, 220, 613
MlyI GAGTC 3 cut(s) 171, 266, 458
MnlI CCTC 8 cut(s) 152, 321, 358, 412, 415, 433, 490, 511
MseI TTAA 1 cut(s) 650
MspR9I CCNGG 2 cut(s) 143, 393
MunI CAATTG 1 cut(s) 220
Mva1269I GAATGC 1 cut(s) 479
MvaI CCWGG 2 cut(s) 143, 393
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 463
NcoI CCATGG 1 cut(s) 261
NdeII GATC 5 cut(s) 199, 208, 276, 400, 577
NlaIII CATG 2 cut(s) 265, 407
NmuCI GTSAC 1 cut(s) 137
PagI TCATGA 1 cut(s) 403
PctI GAATGC 1 cut(s) 479
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 1 cut(s) 461
PleI GAGTC 3 cut(s) 170, 265, 457
PpsI GAGTC 3 cut(s) 170, 265, 457
Ppu21I YACGTR 1 cut(s) 359
PpuMI RGGWCCY 1 cut(s) 424
Psp124BI GAGCTC 2 cut(s) 193, 468
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 141, 391
PspEI GGTNACC 1 cut(s) 137
PspGI CCWGG 2 cut(s) 141, 391
PspPI GGNCC 1 cut(s) 424
PspPPI RGGWCCY 1 cut(s) 424
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 2 cut(s) 193, 468
SaqAI TTAA 1 cut(s) 650
SatI GCNGC 1 cut(s) 460
Sau3AI GATC 5 cut(s) 199, 208, 276, 400, 577
Sau96I GGNCC 1 cut(s) 424
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 3 cut(s) 171, 266, 458
ScrFI CCNGG 2 cut(s) 143, 393
SduI GDGCHC 3 cut(s) 193, 274, 468
SexAI ACCWGGT 1 cut(s) 141
SinI GGWCC 1 cut(s) 424
SnaBI TACGTA 1 cut(s) 359
Sse9I AATT 3 cut(s) 108, 220, 613
SspMI CTAG 1 cut(s) 126
SstI GAGCTC 2 cut(s) 193, 468
StyD4I CCNGG 2 cut(s) 141, 391
StyI CCWWGG 2 cut(s) 261, 427
TaaI ACNGT 2 cut(s) 22, 283
TaiI ACGT 1 cut(s) 361
TaqI TCGA 2 cut(s) 384, 492
TasI AATT 3 cut(s) 108, 220, 613
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 1 cut(s) 650
Tru9I TTAA 1 cut(s) 650
TscAI CASTG 5 cut(s) 100, 209, 279, 288, 420
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 1 cut(s) 459
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 4 cut(s) 43, 66, 420, 502
TspRI CASTG 5 cut(s) 100, 209, 279, 288, 420
VpaK11BI GGWCC 1 cut(s) 424
XapI RAATTY 1 cut(s) 613
XspI CTAG 1 cut(s) 126
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.