Rw6G039480

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016772
Physical Location & Seq
Reverse (-)
63473003 .. 63473560
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039480.1

Sequence Viewer

Length: 558 bp
ATGGGGATCACTGGAGTGTGCTCTAGGTTGATTGGTGACTTGGTTCATGCCTTTCATGGAGTCTCAAGTTGCTCAATCACAGAAGCCCGAATTGATGAAGATTGCAAGCCACAAAGTGCAATCAAAGTTGACGAGTCTATGGCAAGGGCACTCATCACTGTGTTTGGGATGGAAACCAATGGCAGAATAAAGAAGGAGAAGGCCAAGAGAGTGGTGGAGAATCTTGGGTTGATATGTTACGATGAGGAAGACGAAGACAAGTCAAAGTTCGACTTGCCCGGGGATAATGATCATGATGAAGTGCCAGTGGAAGAAGTTCTTGGTGAGTTGGGGAATGATGATGGTTCTAAGAGAAATGAAATGCTGCTCGAAGCGTTCAAGATTTTCGATGAGGATGGAGACGGGTTTATAGAAGCTGTGGAGTTAAAGAGGGTGTTGGAATGTTTAGGGTTGGATAATGGGTGGGATATGGCTCAGATTGAGAAGATGGTGAAGGTTGTGGATTTGAATTTGGATGGGAAGGTTGATTTTAGTGAGTTTGAATTAATGATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

185

Amino Acids

20.57

Weight (kDa)

4.36

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 121 - 184 8.9e-14 EF-hand domain pair
EF-hand_1 PF00036 122 - 147 4.6e-06 EF hand domain
EF-hand_6 PF13405 122 - 150 1.3e-07 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016772)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36371
malus_domestica MD08G1123200.v1.1 MD15G1102200.v1.1
prunus_persica Prupe.1G455200_v2.0.a1
pyrus_communis pycom15g09340
rosa_laevigata RLG00000010883
rosa_multiflora Rmu_sc0001809.1_g000003
rosa_roxburghii Rroxscaffold_7G00162730
rosa_rugosa Rorug06G0340900
rosa_samantha Rh6AG453100 Rh6BG448700 Rh6CG466800 Rh6DG453500
rosa_wichuraiana Rw6G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 14
AcsI RAATTY 1 cut(s) 508
AdeI CACNNNGTG 1 cut(s) 116
AgsI TTSAA 3 cut(s) 379, 508, 542
AjuI GAANNNNNNNTTGG 2 cut(s) 303, 335
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 1 cut(s) 416
AluI AGCT 1 cut(s) 416
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 2 cut(s) 67, 393
AlwI GGATC 1 cut(s) 14
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 1 cut(s) 364
ApoI RAATTY 1 cut(s) 508
AseI ATTAAT 1 cut(s) 545
Asp700I GAANNNNTTC 1 cut(s) 315
AsuC2I CCSGG 2 cut(s) 279, 280
AsuHPI GGTGA 3 cut(s) 47, 335, 502
AvaI CYCGRG 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 151
BbsI GAAGAC 2 cut(s) 255, 261
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 1 cut(s) 351
BccI CCATC 6 cut(s) 163, 335, 389, 481, 509, 544
BclI TGATCA 1 cut(s) 289
BcnI CCSGG 2 cut(s) 279, 280
BcoDI GTCTC 2 cut(s) 67, 393
BfaI CTAG 1 cut(s) 24
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 2 cut(s) 279, 280
BmeT110I CYCGRG 1 cut(s) 278
BmrFI CCNGG 2 cut(s) 279, 280
BpiI GAAGAC 2 cut(s) 255, 261
BpmI CTGGAG 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 49
BpuMI CCSGG 2 cut(s) 279, 280
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 278, 279
Bse1I ACTGG 2 cut(s) 16, 305
Bse8I GATNNNNATC 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 278, 279
BseGI GGATG 3 cut(s) 174, 400, 520
BseJI GATNNNNATC 1 cut(s) 288
BseMII CTCAG 1 cut(s) 488
BseNI ACTGG 2 cut(s) 16, 305
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 1 cut(s) 351
BshFI GGCC 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 67, 393
BsmBI CGTCTC 1 cut(s) 393
BsnI GGCC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 23, 151
Bsp143I GATC 2 cut(s) 6, 289
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 1 cut(s) 487
BspHI TCATGA 1 cut(s) 292
BspPI GGATC 1 cut(s) 14
BsrI ACTGG 2 cut(s) 16, 305
BssECI CCNNGG 2 cut(s) 278, 279
BssMI GATC 2 cut(s) 6, 289
Bst4CI ACNGT 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 2 cut(s) 348, 474
BstF5I GGATG 3 cut(s) 174, 400, 520
BstKTI GATC 2 cut(s) 9, 292
BstMAI GTCTC 2 cut(s) 67, 393
BstMBI GATC 2 cut(s) 6, 289
BstSCI CCNGG 2 cut(s) 277, 278
BstSLI GKGCMC 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 351
BstV2I GAAGAC 2 cut(s) 255, 261
BstXI CCANNNNNNTGG 1 cut(s) 211
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 3 cut(s) 174, 400, 520
BtsIMutI CAGTG 3 cut(s) 9, 156, 312
Cac8I GCNNGC 1 cut(s) 107
CciI TCATGA 1 cut(s) 292
Cfr9I CCCGGG 1 cut(s) 278
CviAII CATG 3 cut(s) 47, 56, 293
CviJI RGCY 5 cut(s) 86, 109, 203, 416, 473
CviKI_1 RGCY 5 cut(s) 86, 109, 203, 416, 473
DdeI CTNAG 2 cut(s) 348, 474
DpnI GATC 2 cut(s) 8, 291
DpnII GATC 2 cut(s) 6, 289
DraIII CACNNNGTG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 278
Esp3I CGTCTC 1 cut(s) 393
FaeI CATG 3 cut(s) 50, 59, 296
FaiI YATR 7 cut(s) 48, 57, 140, 235, 294, 410, 470
FalI AAGNNNNNCTT 4 cut(s) 257, 289, 303, 335
FatI CATG 3 cut(s) 46, 55, 292
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 365
FokI GGATG 3 cut(s) 181, 407, 527
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 1 cut(s) 24
GluI GCNGC 1 cut(s) 365
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 1 cut(s) 203
HapII CCGG 1 cut(s) 279
Hin1II CATG 3 cut(s) 50, 59, 296
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 3 cut(s) 60, 134, 220
HpaII CCGG 1 cut(s) 279
HphI GGTGA 3 cut(s) 47, 335, 502
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 477
Hpy188III TCNNGA 2 cut(s) 293, 379
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 4 cut(s) 187, 193, 487, 514
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 2 cut(s) 105, 119
HpyF3I CTNAG 2 cut(s) 348, 474
Hsp92II CATG 3 cut(s) 50, 59, 296
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 2 cut(s) 6, 289
LpnPI CCDG 2 cut(s) 292, 318
Lsp1109I GCAGC 1 cut(s) 351
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 2 cut(s) 35, 236
MalI GATC 2 cut(s) 8, 291
MboI GATC 2 cut(s) 6, 289
MboII GAAGA 5 cut(s) 110, 260, 266, 323, 496
MhlI GDGCHC 2 cut(s) 23, 151
MluCI AATT 3 cut(s) 90, 508, 542
MlyI GAGTC 2 cut(s) 69, 143
MmeI TCCRAC 2 cut(s) 417, 432
MnlI CCTC 3 cut(s) 238, 385, 423
MroXI GAANNNNTTC 1 cut(s) 315
MseI TTAA 2 cut(s) 425, 545
MslI CAYNNNNRTG 2 cut(s) 14, 158
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 2 cut(s) 279, 280
NciI CCSGG 2 cut(s) 279, 280
NdeII GATC 2 cut(s) 6, 289
NlaIII CATG 3 cut(s) 50, 59, 296
NmuCI GTSAC 1 cut(s) 35
OliI CACNNNNGTG 1 cut(s) 14
PagI TCATGA 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 220
PkrI GCNGC 1 cut(s) 366
PleI GAGTC 2 cut(s) 68, 142
PpsI GAGTC 2 cut(s) 68, 142
PshBI ATTAAT 1 cut(s) 545
RseI CAYNNNNRTG 2 cut(s) 14, 158
SaqAI TTAA 2 cut(s) 425, 545
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 2 cut(s) 6, 289
SchI GAGTC 2 cut(s) 69, 143
ScrFI CCNGG 2 cut(s) 279, 280
SduI GDGCHC 2 cut(s) 23, 151
SetI ASST 4 cut(s) 29, 418, 498, 525
SmaI CCCGGG 1 cut(s) 280
SmiMI CAYNNNNRTG 2 cut(s) 14, 158
SmlI CTYRAG 1 cut(s) 64
SmoI CTYRAG 1 cut(s) 64
Sse9I AATT 3 cut(s) 90, 508, 542
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 277, 278
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 270, 369, 387
TasI AATT 3 cut(s) 90, 508, 542
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 2 cut(s) 425, 545
Tru9I TTAA 2 cut(s) 425, 545
TscAI CASTG 3 cut(s) 16, 163, 312
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 5 cut(s) 35, 44, 111, 312, 372
TspMI CCCGGG 1 cut(s) 278
TspRI CASTG 3 cut(s) 16, 163, 312
VspI ATTAAT 1 cut(s) 545
XapI RAATTY 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmaI CCCGGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 315
XspI CTAG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.