Rorug06G0340900

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0016772
Physical Location & Seq
Reverse (-)
49151593 .. 49155329
3737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0340900.1

Sequence Viewer

Length: 1080 bp
ATGACAACAATTACGGAGGCCGAGGATTTCCATTTAAATTCTCGCTCACTGTTTCTCTCTGAGCTGAGAAGAAGTGTTTTGGGGTTTAATTTGAAGATAAGGAGGAGGAGGAGGAGGAAGAAGATGAATAGGCAGCAGCAGGCGTCGCACTACTGGTTGTTTGACAGTCGCAGCAATTCGAGGAGGTCACCATGGCTTCAATCGACTCTGGCTGAGCTAGAAAAGAATACGGAGGCCATGCTAAAGCTGATTCAGGAAGATGCAGATTCCTTTGCCCAACGTGCAGAGATGTATTACAAGAAGAGACCACAGCTAATCGGCATGGTTGAAGAATTTTATCGAGCTCACAGGTCATTAGCTGAGAGATATGATCTAGTTAAGTCTGATTCGGGAACGCGCCTCCTGACAACATTAGGGTCTCCATTCTCAACCAAATGCCATTCAGAGAAGTCGATGAGTGTGGTGGACGAAACCTACGACAGCCTCTCCGAAACCTGTGAACCTGAGGAGTACACCGAGTCTGAAGTTGATGATCCCGACCTAGAGGATGAAACTTCAGTTGATAAAGTAACAGAAGAGAAGGTTTCATGTGGGGTTAGTGACGATGAAGTGAGAAAGTTAAGGGAAGAAATAGAGAGGCTTCAGGAAGAGAATAGAATTCAAAAGGATCAACTAAAGCAGAAAGATGATGAGAAGAGAGAGGTAATAAGACAGTTGAGTATGGCTGTGGATGTTTACAAACAGGAGAATGTGAAGCTGAAGAAGTATCTTGCCCGAGAATCAGCAGATAATTCGAGTCCCTTCTTCGAGTTTAACAAATTAAAGGATGGTGAAAAACCCAATTCTGGTTCAGGAGCAGCAACAGTAGCTTCAGGGTTTGGTGGGCTTGGGCTTGATGGAATTGTGGGCAGTGATGACTCACTTGGAGAAATTGGCACACCAGAAGGTGAAGGAAACAAAGGAGAAATATCTGGGGATAAGGTTGGAGAGGAAGATAGTGGGGCTGGAGCATCTGGTAAAGCTGCAGGTGCTGCAGAGATTGAGGAAATCTTAGTAGCTAGTGAAGAAGAAGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

359

Amino Acids

40.46

Weight (kDa)

4.84

Isoelectric Point (pI)

73.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 52 - 124 3.4e-32 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016772)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36371
malus_domestica MD08G1123200.v1.1 MD15G1102200.v1.1
prunus_persica Prupe.1G455200_v2.0.a1
pyrus_communis pycom15g09340
rosa_laevigata RLG00000010883
rosa_multiflora Rmu_sc0001809.1_g000003
rosa_roxburghii Rroxscaffold_7G00162730
rosa_rugosa Rorug06G0340900
rosa_samantha Rh6AG453100 Rh6BG448700 Rh6CG466800 Rh6DG453500
rosa_wichuraiana Rw6G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1016
Acc36I ACCTGC 1 cut(s) 1016
AccII CGCG 1 cut(s) 397
AclWI GGATC 2 cut(s) 527, 675
AcsI RAATTY 3 cut(s) 37, 332, 657
AcuI CTGAAG 5 cut(s) 540, 543, 626, 779, 855
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 1 cut(s) 845
AgsI TTSAA 4 cut(s) 94, 200, 329, 662
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 2 cut(s) 298, 423
AlwI GGATC 2 cut(s) 527, 675
AlwNI CAGNNNCTG 1 cut(s) 1031
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 2 cut(s) 18, 234
ApeKI GCWGC 6 cut(s) 133, 136, 171, 857, 1022, 1031
ApoI RAATTY 3 cut(s) 37, 332, 657
AspLEI GCGC 1 cut(s) 399
AsuHPI GGTGA 3 cut(s) 180, 842, 959
AvaI CYCGRG 1 cut(s) 774
AxyI CCTNAGG 1 cut(s) 504
BanII GRGCYC 1 cut(s) 346
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 6 cut(s) 145, 148, 183, 869, 1009, 1018
BccI CCATC 2 cut(s) 821, 890
BcgI CGANNNNNNTGC 2 cut(s) 774, 808
BcoDI GTCTC 2 cut(s) 298, 423
BfaI CTAG 4 cut(s) 218, 374, 542, 1059
BfmI CTRYAG 2 cut(s) 1023, 1032
BfuAI ACCTGC 1 cut(s) 1016
BisI GCNGC 6 cut(s) 134, 137, 172, 858, 1023, 1032
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 6 cut(s) 135, 138, 173, 859, 1024, 1033
BmeT110I CYCGRG 1 cut(s) 774
BmsI GCATC 2 cut(s) 250, 1019
BpmI CTGGAG 1 cut(s) 1026
Bpu1102I GCTNAGC 1 cut(s) 213
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 2 cut(s) 298, 423
BsaJI CCNNGG 2 cut(s) 21, 191
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 1 cut(s) 845
Bse1I ACTGG 1 cut(s) 158
Bse21I CCTNAGG 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 21, 191
BseGI GGATG 3 cut(s) 553, 736, 832
BseLI CCNNNNNNNGG 1 cut(s) 845
BseMII CTCAG 5 cut(s) 51, 56, 204, 351, 495
BseNI ACTGG 1 cut(s) 158
BseRI GAGGAG 6 cut(s) 118, 121, 124, 127, 196, 521
BseXI GCAGC 6 cut(s) 145, 148, 183, 869, 1009, 1018
BsgI GTGCAG 1 cut(s) 303
Bsh1236I CGCG 1 cut(s) 397
BshFI GGCC 2 cut(s) 20, 236
BsiHKAI GWGCWC 1 cut(s) 346
BsiHKCI CYCGRG 1 cut(s) 774
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 1 cut(s) 845
BsmAI GTCTC 2 cut(s) 298, 423
BsmFI GGGAC 1 cut(s) 783
BsnI GGCC 2 cut(s) 20, 236
Bso31I GGTCTC 2 cut(s) 298, 423
BsoBI CYCGRG 1 cut(s) 774
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 3 cut(s) 370, 532, 667
Bsp1720I GCTNAGC 1 cut(s) 213
Bsp19I CCATGG 1 cut(s) 191
BspANI GGCC 2 cut(s) 20, 236
BspCNI CTCAG 5 cut(s) 52, 57, 205, 352, 496
BspFNI CGCG 1 cut(s) 397
BspMAI CTGCAG 2 cut(s) 1027, 1036
BspMI ACCTGC 1 cut(s) 1016
BspPI GGATC 2 cut(s) 527, 675
BspTNI GGTCTC 2 cut(s) 298, 423
BsrI ACTGG 1 cut(s) 158
BssECI CCNNGG 2 cut(s) 21, 191
BssMI GATC 3 cut(s) 370, 532, 667
BssNI GRCGYC 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 191
Bst4CI ACNGT 4 cut(s) 51, 167, 714, 865
Bst6I CTCTTC 4 cut(s) 296, 570, 642, 689
BstACI GRCGYC 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 6 cut(s) 60, 65, 213, 360, 504, 1051
BstDSI CCRYGG 1 cut(s) 191
BstEII GGTNACC 1 cut(s) 186
BstF5I GGATG 3 cut(s) 553, 736, 832
BstFNI CGCG 1 cut(s) 397
BstHHI GCGC 1 cut(s) 399
BstKTI GATC 3 cut(s) 373, 535, 670
BstMAI GTCTC 2 cut(s) 298, 423
BstMBI GATC 3 cut(s) 370, 532, 667
BstMWI GCNNNNNNNGC 5 cut(s) 145, 281, 866, 1028, 1031
BstPI GGTNACC 1 cut(s) 186
BstSFI CTRYAG 2 cut(s) 1023, 1032
BstUI CGCG 1 cut(s) 397
BstV1I GCAGC 6 cut(s) 145, 148, 183, 869, 1009, 1018
Bsu36I CCTNAGG 1 cut(s) 504
BsuRI GGCC 2 cut(s) 20, 236
BtgI CCRYGG 1 cut(s) 191
BtsCI GGATG 3 cut(s) 553, 736, 832
BtsI GCAGTG 1 cut(s) 916
BtsIMutI CAGTG 2 cut(s) 47, 916
BveI ACCTGC 1 cut(s) 1016
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 1 cut(s) 399
CseI GACGC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 511
CviAII CATG 4 cut(s) 192, 238, 322, 588
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 6 cut(s) 60, 65, 213, 360, 504, 1051
DpnI GATC 3 cut(s) 372, 534, 669
DpnII GATC 3 cut(s) 370, 532, 667
DraI TTTAAA 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 296, 570, 642, 689
EarI CTCTTC 4 cut(s) 296, 570, 642, 689
Ecl136II GAGCTC 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 346
Eco31I GGTCTC 2 cut(s) 298, 423
Eco53kI GAGCTC 1 cut(s) 344
Eco57I CTGAAG 5 cut(s) 540, 543, 626, 779, 855
Eco81I CCTNAGG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 774
Eco91I GGTNACC 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 344
EcoO65I GGTNACC 1 cut(s) 186
EcoRI GAATTC 1 cut(s) 657
EcoT14I CCWWGG 1 cut(s) 191
EcoT38I GRGCYC 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 4 cut(s) 195, 241, 325, 591
FaiI YATR 6 cut(s) 193, 239, 323, 369, 589, 722
FaqI GGGAC 1 cut(s) 783
FatI CATG 4 cut(s) 191, 237, 321, 587
Fnu4HI GCNGC 6 cut(s) 134, 137, 172, 858, 1023, 1032
FokI GGATG 3 cut(s) 560, 743, 839
FriOI GRGCYC 1 cut(s) 346
Fsp4HI GCNGC 6 cut(s) 134, 137, 172, 858, 1023, 1032
FspBI CTAG 4 cut(s) 218, 374, 542, 1059
GlaI GCGC 1 cut(s) 398
GluI GCNGC 6 cut(s) 134, 137, 172, 858, 1023, 1032
GsuI CTGGAG 1 cut(s) 1026
HaeIII GGCC 2 cut(s) 20, 236
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 4 cut(s) 195, 241, 325, 591
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HinfI GANTC 8 cut(s) 205, 250, 266, 386, 518, 779, 796, 917
HphI GGTGA 3 cut(s) 180, 842, 959
Hpy166II GTNNAC 4 cut(s) 466, 500, 513, 736
Hpy188I TCNGA 5 cut(s) 61, 385, 445, 490, 523
Hpy188III TCNNGA 6 cut(s) 254, 390, 403, 536, 644, 852
Hpy8I GTNNAC 4 cut(s) 466, 500, 513, 736
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 4 cut(s) 574, 811, 938, 944
HpyCH4III ACNGT 4 cut(s) 51, 167, 714, 865
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 4 cut(s) 263, 284, 1025, 1034
HpyF10VI GCNNNNNNNGC 5 cut(s) 145, 281, 866, 1028, 1031
HpyF3I CTNAG 6 cut(s) 60, 65, 213, 360, 504, 1051
HpySE526I ACGT 1 cut(s) 280
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 4 cut(s) 195, 241, 325, 591
HspAI GCGC 1 cut(s) 397
Kzo9I GATC 3 cut(s) 370, 532, 667
LmnI GCTCC 2 cut(s) 854, 1007
Lsp1109I GCAGC 6 cut(s) 145, 148, 183, 869, 1009, 1018
LweI GCATC 2 cut(s) 250, 1019
MaeI CTAG 4 cut(s) 218, 374, 542, 1059
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 3 cut(s) 186, 568, 599
MalI GATC 3 cut(s) 372, 534, 669
MboI GATC 3 cut(s) 370, 532, 667
MhlI GDGCHC 1 cut(s) 346
MlyI GAGTC 4 cut(s) 199, 527, 805, 911
MmeI TCCRAC 1 cut(s) 964
MseI TTAA 6 cut(s) 35, 87, 378, 620, 813, 821
MvnI CGCG 1 cut(s) 397
MwoI GCNNNNNNNGC 5 cut(s) 145, 281, 866, 1028, 1031
NcoI CCATGG 1 cut(s) 191
NdeII GATC 3 cut(s) 370, 532, 667
NlaIII CATG 4 cut(s) 195, 241, 325, 591
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 2 cut(s) 186, 599
PaqCI CACCTGC 1 cut(s) 1016
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PfeI GAWTC 4 cut(s) 250, 266, 386, 779
PkrI GCNGC 6 cut(s) 135, 138, 173, 859, 1024, 1033
PleI GAGTC 4 cut(s) 199, 526, 804, 911
PpsI GAGTC 4 cut(s) 199, 526, 804, 911
Psp124BI GAGCTC 1 cut(s) 346
PspEI GGTNACC 1 cut(s) 186
PstI CTGCAG 2 cut(s) 1027, 1036
PstNI CAGNNNCTG 1 cut(s) 1031
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SacI GAGCTC 1 cut(s) 346
SaqAI TTAA 6 cut(s) 35, 87, 378, 620, 813, 821
SatI GCNGC 6 cut(s) 134, 137, 172, 858, 1023, 1032
Sau3AI GATC 3 cut(s) 370, 532, 667
SchI GAGTC 4 cut(s) 199, 527, 805, 911
SduI GDGCHC 1 cut(s) 346
SfaNI GCATC 2 cut(s) 250, 1019
SfcI CTRYAG 2 cut(s) 1023, 1032
SmiI ATTTAAAT 1 cut(s) 36
SspMI CTAG 4 cut(s) 218, 374, 542, 1059
SstI GAGCTC 1 cut(s) 346
StyI CCWWGG 1 cut(s) 191
SwaI ATTTAAAT 1 cut(s) 36
TaaI ACNGT 4 cut(s) 51, 167, 714, 865
TaiI ACGT 1 cut(s) 283
TaqI TCGA 6 cut(s) 179, 203, 340, 452, 794, 807
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 4 cut(s) 250, 266, 386, 779
Tru1I TTAA 6 cut(s) 35, 87, 378, 620, 813, 821
Tru9I TTAA 6 cut(s) 35, 87, 378, 620, 813, 821
TscAI CASTG 2 cut(s) 54, 916
TseFI GTSAC 2 cut(s) 186, 599
TseI GCWGC 6 cut(s) 133, 136, 171, 857, 1022, 1031
Tsp45I GTSAC 2 cut(s) 186, 599
TspDTI ATGAA 4 cut(s) 140, 564, 576, 621
TspGWI ACGGA 2 cut(s) 29, 245
TspRI CASTG 2 cut(s) 54, 916
XapI RAATTY 3 cut(s) 37, 332, 657
XspI CTAG 4 cut(s) 218, 374, 542, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.