Prupe.1G455200_v2.0.a1

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016772
Physical Location & Seq
Forward (+)
38230572 .. 38231281
710 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G455200.1

Sequence Viewer

Length: 555 bp
ATGGGAATAACTGGAGTTTGCTCTAGGTTACTTGGGGACTTGGTTCAAGCCTTTGGAGGGGTCTCAAGGCCAAGCACAGAATGCAAGCCACAAAATGGCAGTTGCAAGCCACAAAATGCAAGCAAAGTTGATGAGTCTATGGCAAGGGCACTGATCACAGTGTTTGGGATGGAAACCAACGGAAGAATCAAGAAGGAAAACGCACGGAGGGTGGTGGAGAATCTAGGGTTGATATGTCATGAGGAAGACAAGTCGAGTTTTGACTTGCCAGGGGATGATCATGAGGTGCCAGTGGAGGAGGTTCTTGGTGGTTTGGAGGAGGAGGACGATGGGTCCAAGCGCAATGAGCTTTTGCTCGAGGCATTCAAGATTTTTGATGAGGATGGTGATGGGTTTATAGAGGCTGTGGAGTTGAAGAGGGTGTTGGAGTGTTTGGGGTTGGGGAGTGGGTGGGATATGGATCAGATTGAGAAGATGGTGAGGGTTGTGGATGTGAATTTGGATGGGAAGGTTGATTTTAATGAGTTTGAGTTGATGATGGGGGTAAAATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

185

Amino Acids

20.26

Weight (kDa)

4.48

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016772)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36371
malus_domestica MD08G1123200.v1.1 MD15G1102200.v1.1
prunus_persica Prupe.1G455200_v2.0.a1
pyrus_communis pycom15g09340
rosa_laevigata RLG00000010883
rosa_multiflora Rmu_sc0001809.1_g000003
rosa_roxburghii Rroxscaffold_7G00162730
rosa_rugosa Rorug06G0340900
rosa_samantha Rh6AG453100 Rh6BG448700 Rh6CG466800 Rh6DG453500
rosa_wichuraiana Rw6G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 95
AclWI GGATC 1 cut(s) 468
AcsI RAATTY 1 cut(s) 496
AfiI CCNNNNNNNGG 2 cut(s) 57, 95
AgsI TTSAA 3 cut(s) 47, 367, 415
AhdI GACNNNNNGTC 1 cut(s) 331
AjnI CCWGG 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 1 cut(s) 349
AluI AGCT 1 cut(s) 349
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 1 cut(s) 468
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 1 cut(s) 68
ApoI RAATTY 1 cut(s) 496
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 2 cut(s) 398, 490
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 1 cut(s) 333
BaeGI GKGCMC 1 cut(s) 151
BanI GGYRCC 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 252
BccI CCATC 7 cut(s) 163, 323, 377, 383, 469, 497, 532
BciT130I CCWGG 1 cut(s) 270
BclI TGATCA 2 cut(s) 153, 277
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 2 cut(s) 24, 224
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 1 cut(s) 333
BmeRI GACNNNNNGTC 1 cut(s) 331
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 2 cut(s) 288, 334
BmrFI CCNGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 269
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 95
Bse1I ACTGG 2 cut(s) 16, 290
Bse3DI GCAATG 1 cut(s) 349
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 269
BseGI GGATG 5 cut(s) 174, 280, 388, 496, 508
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 57, 95
BseMI GCAATG 1 cut(s) 349
BseNI ACTGG 2 cut(s) 16, 290
BseRI GAGGAG 3 cut(s) 311, 332, 335
BseSI GKGCMC 1 cut(s) 151
BshFI GGCC 1 cut(s) 70
BshNI GGYRCC 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 356
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 57, 95
BsmAI GTCTC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 2 cut(s) 86, 362
BsnI GGCC 1 cut(s) 70
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 151
Bsp143I GATC 3 cut(s) 153, 277, 460
BspANI GGCC 1 cut(s) 70
BspHI TCATGA 2 cut(s) 238, 280
BspLI GGNNCC 2 cut(s) 288, 334
BspPI GGATC 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 2 cut(s) 16, 290
BssECI CCNNGG 1 cut(s) 269
BssMI GATC 3 cut(s) 153, 277, 460
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 160
Bst6I CTCTTC 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 81
BstC8I GCNNGC 3 cut(s) 86, 107, 121
BstF5I GGATG 5 cut(s) 174, 280, 388, 496, 508
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 3 cut(s) 156, 280, 463
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 3 cut(s) 153, 277, 460
BstMWI GCNNNNNNNGC 2 cut(s) 81, 346
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstSLI GKGCMC 1 cut(s) 151
BstV2I GAAGAC 1 cut(s) 252
BsuRI GGCC 1 cut(s) 70
BtsCI GGATG 5 cut(s) 174, 280, 388, 496, 508
BtsIMutI CAGTG 3 cut(s) 149, 165, 297
Cac8I GCNNGC 3 cut(s) 86, 107, 121
CciI TCATGA 2 cut(s) 238, 280
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 333
CviAII CATG 2 cut(s) 239, 281
CviJI RGCY 6 cut(s) 50, 70, 88, 109, 349, 404
CviKI_1 RGCY 6 cut(s) 50, 70, 88, 109, 349, 404
DpnI GATC 3 cut(s) 155, 279, 462
DpnII GATC 3 cut(s) 153, 277, 460
DriI GACNNNNNGTC 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 410
Eam1105I GACNNNNNGTC 1 cut(s) 331
EarI CTCTTC 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 1 cut(s) 333
Eco88I CYCGRG 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 268
FaeI CATG 2 cut(s) 242, 284
FaiI YATR 6 cut(s) 140, 235, 240, 282, 398, 458
FaqI GGGAC 1 cut(s) 50
FatI CATG 2 cut(s) 238, 280
FbaI TGATCA 2 cut(s) 153, 277
FokI GGATG 5 cut(s) 181, 287, 395, 503, 515
FspBI CTAG 2 cut(s) 24, 224
GlaI GCGC 1 cut(s) 341
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 1 cut(s) 70
HhaI GCGC 1 cut(s) 342
Hin1II CATG 2 cut(s) 242, 284
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HinfI GANTC 3 cut(s) 134, 186, 220
HphI GGTGA 2 cut(s) 398, 490
Hpy188I TCNGA 1 cut(s) 465
Hpy188III TCNNGA 4 cut(s) 190, 239, 281, 367
HpyAV CCTTC 2 cut(s) 187, 502
HpyCH4III ACNGT 1 cut(s) 160
HpyCH4V TGCA 3 cut(s) 84, 105, 119
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 346
Hsp92II CATG 2 cut(s) 242, 284
HspAI GCGC 1 cut(s) 340
Ksp22I TGATCA 2 cut(s) 153, 277
Kzo9I GATC 3 cut(s) 153, 277, 460
LpnPI CCDG 3 cut(s) 255, 282, 303
MaeI CTAG 2 cut(s) 24, 224
MaeIII GTNAC 1 cut(s) 27
MalI GATC 3 cut(s) 155, 279, 462
MboI GATC 3 cut(s) 153, 277, 460
MboII GAAGA 4 cut(s) 195, 257, 427, 484
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 1 cut(s) 496
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 405
MseI TTAA 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 270
Mva1269I GAATGC 2 cut(s) 86, 362
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 2 cut(s) 81, 346
NdeII GATC 3 cut(s) 153, 277, 460
NlaIII CATG 2 cut(s) 242, 284
NlaIV GGNNCC 2 cut(s) 288, 334
PaeR7I CTCGAG 1 cut(s) 356
PagI TCATGA 2 cut(s) 238, 280
PctI GAATGC 2 cut(s) 86, 362
PfeI GAWTC 2 cut(s) 186, 220
PflMI CCANNNNNTGG 1 cut(s) 95
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 288, 334
PspPI GGNCC 1 cut(s) 333
PspXI VCTCGAGB 1 cut(s) 356
SaqAI TTAA 1 cut(s) 519
Sau3AI GATC 3 cut(s) 153, 277, 460
Sau96I GGNCC 1 cut(s) 333
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 151
SetI ASST 5 cut(s) 29, 288, 303, 351, 513
Sfr274I CTCGAG 1 cut(s) 356
SinI GGWCC 1 cut(s) 333
SlaI CTCGAG 1 cut(s) 356
SmlI CTYRAG 2 cut(s) 64, 356
SmoI CTYRAG 2 cut(s) 64, 356
Sse9I AATT 1 cut(s) 496
SspMI CTAG 2 cut(s) 24, 224
StyD4I CCNGG 1 cut(s) 268
TaaI ACNGT 1 cut(s) 160
TaqI TCGA 2 cut(s) 254, 357
TasI AATT 1 cut(s) 496
TfiI GAWTC 2 cut(s) 186, 220
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 3 cut(s) 156, 165, 297
TspGWI ACGGA 2 cut(s) 195, 220
TspRI CASTG 3 cut(s) 156, 165, 297
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 333
XapI RAATTY 1 cut(s) 496
XhoI CTCGAG 1 cut(s) 356
XspI CTAG 2 cut(s) 24, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.