MD15G1102200.v1.1

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016772
Physical Location & Seq
Forward (+)
7235426 .. 7235989
564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1102200.v1.1.491

Sequence Viewer

Length: 564 bp
ATGATGGGAATTACAGGAGTTTGCTCTAGATTACTTGGTGATTTGGTTCAAGCCATTGGAGGAGTCCCATCAAGTTTAACAAGCAAAGGAGCTCACATTGTTCATGAAGATCAACGTGACCAATATTCCAAATGCAAACCACAAAATATTGCAACCAAAGTTGATGAGTCCATGGCAAGGGCACTGATCACAGTGTTTGGGATGGAAACAAATGGAAGAATCAAGAAGGAAAAGGCGCGGCGTGTTGTGGAGAATCTTGGACTAATACGTAATGAGGTAGACCAGACGGGCTTCGATTTGCCGGGGGATGATCATGAAGAGGAGGTCCTTGGTGGATTGGAGGATAATGAGTTCGAGCGCAACGAGCTCCTGCGCGAAGCATTCAAGATTTTCGACGAGGATGGCAATGGATTCATAGAGGCTGTGGAGTTGAAAAGGGTTTTGGAATGCTTGGGTTTGGGGAGTGGGTGGGAGATGGGCCAGGTTGAGAAGATGGTGAAGGTTGTGGATTTGAATATGGATGGGAAGGTTGATTTTCGGGAGTTTGAGTTAATGATGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

188

Amino Acids

20.85

Weight (kDa)

4.72

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 124 - 149 2.6e-06 EF hand domain
EF-hand_7 PF13499 124 - 186 2.1e-12 EF-hand domain pair
EF-hand_6 PF13405 124 - 152 4e-08 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016772)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36371
malus_domestica MD08G1123200.v1.1 MD15G1102200.v1.1
prunus_persica Prupe.1G455200_v2.0.a1
pyrus_communis pycom15g09340
rosa_laevigata RLG00000010883
rosa_multiflora Rmu_sc0001809.1_g000003
rosa_roxburghii Rroxscaffold_7G00162730
rosa_rugosa Rorug06G0340900
rosa_samantha Rh6AG453100 Rh6BG448700 Rh6CG466800 Rh6DG453500
rosa_wichuraiana Rw6G039480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 279
AccII CGCG 2 cut(s) 238, 375
AciI CCGC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 4 cut(s) 50, 385, 433, 514
AjnI CCWGG 1 cut(s) 480
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AloI GAACNNNNNNTCC 2 cut(s) 335, 367
AluBI AGCT 2 cut(s) 92, 367
AluI AGCT 2 cut(s) 92, 367
Alw21I GWGCWC 2 cut(s) 94, 369
AoxI GGCC 1 cut(s) 478
AspLEI GCGC 3 cut(s) 238, 360, 375
AspS9I GGNCC 2 cut(s) 325, 478
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 2 cut(s) 50, 508
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 184
BanII GRGCYC 2 cut(s) 94, 369
Bbv12I GWGCWC 2 cut(s) 94, 369
BccI CCATC 7 cut(s) 76, 196, 395, 469, 487, 515, 550
BciT130I CCWGG 1 cut(s) 482
BclI TGATCA 2 cut(s) 186, 310
BcnI CCSGG 1 cut(s) 303
BfaI CTAG 1 cut(s) 27
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 2 cut(s) 303, 482
Bme18I GGWCC 1 cut(s) 325
BmgT120I GGNCC 2 cut(s) 325, 478
BmrFI CCNGG 2 cut(s) 303, 482
BpuMI CCSGG 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 269
BsaJI CCNNGG 3 cut(s) 171, 302, 328
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 412
BseBI CCWGG 1 cut(s) 482
BseDI CCNNGG 3 cut(s) 171, 302, 328
BseGI GGATG 4 cut(s) 207, 313, 406, 526
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 1 cut(s) 412
BseRI GAGGAG 2 cut(s) 75, 335
BseSI GKGCMC 1 cut(s) 184
Bsh1236I CGCG 2 cut(s) 238, 375
BshFI GGCC 1 cut(s) 480
BsiHKAI GWGCWC 2 cut(s) 94, 369
BsiSI CCGG 1 cut(s) 302
BslFI GGGAC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 50
BsmI GAATGC 2 cut(s) 380, 452
BsnI GGCC 1 cut(s) 480
Bsp1286I GDGCHC 3 cut(s) 94, 184, 369
Bsp143I GATC 3 cut(s) 109, 186, 310
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 1 cut(s) 238
BspANI GGCC 1 cut(s) 480
BspFNI CGCG 2 cut(s) 238, 375
BspHI TCATGA 2 cut(s) 103, 313
BsrDI GCAATG 1 cut(s) 412
BssECI CCNNGG 3 cut(s) 171, 302, 328
BssMI GATC 3 cut(s) 109, 186, 310
BssT1I CCWWGG 2 cut(s) 171, 328
Bst2UI CCWGG 1 cut(s) 482
Bst4CI ACNGT 1 cut(s) 193
Bst6I CTCTTC 1 cut(s) 312
BstBAI YACGTR 1 cut(s) 269
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 4 cut(s) 207, 313, 406, 526
BstFNI CGCG 2 cut(s) 238, 375
BstHHI GCGC 3 cut(s) 238, 360, 375
BstKTI GATC 3 cut(s) 112, 189, 313
BstMBI GATC 3 cut(s) 109, 186, 310
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNI CCWGG 1 cut(s) 482
BstSCI CCNGG 2 cut(s) 301, 480
BstSLI GKGCMC 1 cut(s) 184
BstSNI TACGTA 1 cut(s) 269
BstUI CGCG 2 cut(s) 238, 375
BsuRI GGCC 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 171
BtsCI GGATG 4 cut(s) 207, 313, 406, 526
BtsIMutI CAGTG 2 cut(s) 182, 198
CciI TCATGA 2 cut(s) 103, 313
CfoI GCGC 3 cut(s) 238, 360, 375
Cfr13I GGNCC 2 cut(s) 325, 478
CviAII CATG 3 cut(s) 104, 172, 314
CviJI RGCY 6 cut(s) 53, 92, 291, 367, 422, 480
CviKI_1 RGCY 6 cut(s) 53, 92, 291, 367, 422, 480
DpnI GATC 3 cut(s) 111, 188, 312
DpnII GATC 3 cut(s) 109, 186, 310
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Ecl136II GAGCTC 2 cut(s) 92, 367
Eco105I TACGTA 1 cut(s) 269
Eco130I CCWWGG 2 cut(s) 171, 328
Eco24I GRGCYC 2 cut(s) 94, 369
Eco47I GGWCC 1 cut(s) 325
Eco53kI GAGCTC 2 cut(s) 92, 367
EcoICRI GAGCTC 2 cut(s) 92, 367
EcoO109I RGGNCCY 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 480
EcoT14I CCWWGG 2 cut(s) 171, 328
EcoT38I GRGCYC 2 cut(s) 94, 369
ErhI CCWWGG 2 cut(s) 171, 328
FaeI CATG 3 cut(s) 107, 175, 317
FaiI YATR 5 cut(s) 105, 173, 315, 416, 518
FaqI GGGAC 1 cut(s) 50
FatI CATG 3 cut(s) 103, 171, 313
FbaI TGATCA 2 cut(s) 186, 310
FblI GTMKAC 1 cut(s) 279
Fnu4HI GCNGC 1 cut(s) 239
FokI GGATG 4 cut(s) 214, 320, 413, 533
FriOI GRGCYC 2 cut(s) 94, 369
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 1 cut(s) 27
GlaI GCGC 3 cut(s) 237, 359, 374
GluI GCNGC 1 cut(s) 239
HaeIII GGCC 1 cut(s) 480
HapII CCGG 1 cut(s) 302
HhaI GCGC 3 cut(s) 238, 360, 375
Hin1II CATG 3 cut(s) 107, 175, 317
Hin6I GCGC 3 cut(s) 236, 358, 373
HinP1I GCGC 3 cut(s) 236, 358, 373
HinfI GANTC 5 cut(s) 63, 167, 219, 253, 411
HpaII CCGG 1 cut(s) 302
HphI GGTGA 2 cut(s) 50, 508
Hpy166II GTNNAC 1 cut(s) 280
Hpy188III TCNNGA 6 cut(s) 27, 104, 223, 314, 385, 539
Hpy8I GTNNAC 1 cut(s) 280
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 3 cut(s) 220, 493, 520
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 2 cut(s) 115, 268
HpyCH4V TGCA 2 cut(s) 135, 152
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpySE526I ACGT 2 cut(s) 115, 268
Hsp92II CATG 3 cut(s) 107, 175, 317
HspAI GCGC 3 cut(s) 236, 358, 373
Ksp22I TGATCA 2 cut(s) 186, 310
Kzo9I GATC 3 cut(s) 109, 186, 310
LmnI GCTCC 2 cut(s) 89, 372
LpnPI CCDG 5 cut(s) 296, 315, 383, 467, 494
MaeI CTAG 1 cut(s) 27
MaeII ACGT 2 cut(s) 115, 268
MaeIII GTNAC 1 cut(s) 116
MalI GATC 3 cut(s) 111, 188, 312
MboI GATC 3 cut(s) 109, 186, 310
MboII GAAGA 4 cut(s) 119, 228, 329, 502
MhlI GDGCHC 3 cut(s) 94, 184, 369
MluCI AATT 1 cut(s) 9
MlyI GAGTC 2 cut(s) 72, 176
MnlI CCTC 7 cut(s) 53, 268, 313, 316, 334, 391, 412
MseI TTAA 2 cut(s) 77, 551
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 2 cut(s) 303, 482
Mva1269I GAATGC 2 cut(s) 380, 452
MvaI CCWGG 1 cut(s) 482
MvnI CGCG 2 cut(s) 238, 375
MwoI GCNNNNNNNGC 1 cut(s) 364
NciI CCSGG 1 cut(s) 303
NcoI CCATGG 1 cut(s) 171
NdeII GATC 3 cut(s) 109, 186, 310
NlaIII CATG 3 cut(s) 107, 175, 317
NmuCI GTSAC 1 cut(s) 116
PagI TCATGA 2 cut(s) 103, 313
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 2 cut(s) 380, 452
PfeI GAWTC 3 cut(s) 219, 253, 411
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 2 cut(s) 71, 175
PpsI GAGTC 2 cut(s) 71, 175
Ppu21I YACGTR 1 cut(s) 269
PpuMI RGGWCCY 1 cut(s) 325
Psp124BI GAGCTC 2 cut(s) 94, 369
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 480
PspGI CCWGG 1 cut(s) 480
PspPI GGNCC 2 cut(s) 325, 478
PspPPI RGGWCCY 1 cut(s) 325
SacI GAGCTC 2 cut(s) 94, 369
SaqAI TTAA 2 cut(s) 77, 551
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 3 cut(s) 109, 186, 310
Sau96I GGNCC 2 cut(s) 325, 478
SchI GAGTC 2 cut(s) 72, 176
ScrFI CCNGG 2 cut(s) 303, 482
SduI GDGCHC 3 cut(s) 94, 184, 369
SetI ASST 9 cut(s) 94, 118, 271, 279, 327, 369, 486, 504, 531
SinI GGWCC 1 cut(s) 325
SnaBI TACGTA 1 cut(s) 269
Sse9I AATT 1 cut(s) 9
SsiI CCGC 1 cut(s) 238
SspI AATATT 2 cut(s) 125, 148
SspMI CTAG 1 cut(s) 27
SstI GAGCTC 2 cut(s) 94, 369
StyD4I CCNGG 2 cut(s) 301, 480
StyI CCWWGG 2 cut(s) 171, 328
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 2 cut(s) 118, 271
TaqI TCGA 3 cut(s) 294, 354, 393
TasI AATT 1 cut(s) 9
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 3 cut(s) 219, 253, 411
Tru1I TTAA 2 cut(s) 77, 551
Tru9I TTAA 2 cut(s) 77, 551
TscAI CASTG 2 cut(s) 189, 198
TseFI GTSAC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 4 cut(s) 92, 120, 330, 403
TspRI CASTG 2 cut(s) 189, 198
VpaK11BI GGWCC 1 cut(s) 325
XbaI TCTAGA 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 279
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.