MD08G1130200.v1.1

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006441
Physical Location & Seq
Reverse (-)
12254021 .. 12261215
7195 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1130200.v1.1.491

Sequence Viewer

Length: 2865 bp
ATGGCCGGCGATAAGGCCATCAGCCTCGAAGAGATAAAAAACGAGTCCGTCGATCTGGAACGGATTCCGGTAGAGGAAGTCTTTGAGCAGCTGAAATGTACAAAAGAAGGTCTGACTGGAGAAGAAGGAGCCAGCAGGCTTCAAGTCTTTGGACCAAACAAACTAGAAGAGAAAAAGGAGAGCAAACTGCTCAAGTTCTTGGGATTTATGTGGAATCCTTTGTCATGGGTCATGGAAGCTGCTGCTCTGATGGCTATTGCCTTGGCAAATGGTGGTGGAAAGCCTCCTGATTGGCAGGACTTTGTTGGCATTGTTGTTCTTCTGGTCATTAACTCTACTATCAGTTTCATCGAAGAAAACAATGCTGGTAATGCTGCTGCAGCCCTTATGGCCGGTCTAGCTCCCAAGACTAAGGTTCTTAGAGATGGTCGATGGACTGAGCAAGATGCTTCAATTTTGGTGCCTGGAGACATAATCAGTATCAAATTGGGAGATATTGTTCCTGCTGATGCCCGCCTTCTCGAGGGTGATCCTTTGAAAATTGATCAATCTGCCCTTACTGGGGAGTCCCTTCCTGTTACTAAGAATCCTTCGGATGAAGTGTTTTCTGGCTCAACATGTAAACAGGGTGAAATTGAAGCAGTTGTTATCGCTACTGGTGTGCACACCTTCTTTGGCAAGGCTGCACATCTGGTGGACAACACCAATCAAGTTGGGCACTTCCAGAAAGTTCTCACTGCCATCGGGAACTTCTGCATTTGTTCAATTGCTGTGGGGATACTAATTGAGATAATAGTAATGTACCCAATACAGAAGCGCAAGTACAGGCAAGGGATTGATAATTTGCTGGTTCTCTTGATTGGAGGAATCCCAATTGCCATGCCAACTGTTTTATCAGTCACCATGGCTATTGGCTCCCACAGGCTTTCACAGCAAGGAGCTATCACCAAGAGAATGACTGCCATTGAGGAAATGGCTGGCATGGACGTCCTCTGCAGTGATAAGACTGGGACTTTGACCCTGAACAAGCTCTCAGTTGATAGAAATCTGATTGAAGTGTTTGCCAAGGGTGTGGAGAAAGAGCATGTTATGCTTCTTGCAGCAAGGGCTTCTAGGACTGAAAATCAGGATGCTATTGATGCTGCAATTGTAGGAATGCTTGCTGATCCTAAGGAGGCACGAGCTGGTATAAGAGAAGTCCATTTCCTACCTTTCAACCCTGTAGACAAGAGGACTGCTCTTACCTACATCGATTCTGATGGAAATTGGCATCGCGCTAGTAAAGGTGCCCCTGAGCAGATATTAACCCTATGCAACTGCAAGGAAGATTTCAAGAAAAAGGTGCATGGGGTGATTGATAAGTTTGCTGAACGTGGACTTCGTTCTCTGGGTGTTGCAAGACAGCAAGTACCAGAGAAAACAAAAGAGAGTCCTGGGACACCATGGCAGTTCGTTGGTTTATTGCCGTTATTTGATCCTCCCAGGCATGACAGTGCAGAAACAATTCGCAGGGCTCTCAATCTCGGTGTGAATGTGAAGATGATTACAGGGGATCAGCTTGCCATTGGCAAGGAAACTGGAAGGAGGCTTGGTATGGGAACAAACATGTACCCATCTTCCGCATTGCTTGGCCAAGACAAGGATGCGGCCATAGCTTCCCTCCCTGTGGACGAGTTGATTGAGAAGGCTGATGGATTTGCTGGAGTATTTCCTGAACACAAATATGAAATTGTAAAGAGGCTGCAAGAGAGGAAGCACATCTGTGGAATGACTGGAGATGGTGTCAATGACGCTCCTGCTTTGAAAAAAGCAGATATTGGTATTGCCGTAGCTGATGCTACTGATGCTGCAAGAGGTGCTTCAGATATTGTTCTCACTGAACCAGGGCTAAGTGTGATCATAAGTGCAGTACTGACAAGCAGAGCCATATTCCAGAGGATGAAGAACTATACTATCTATGCTGTTTCAATTACCATACGTATAGTGTTTGGATTTATGTTAATTGCTTTGATATGGAAGTTTGACTTTGCACCCTTCATGGTATTGATCATCGCCATCCTAAATGACGGAACTATCATGACAATCTCAAAGGATCGAGTGAAGCCATCTCCACAACCAGACAGCTGGAAACTGAAGGAGATCTTTGCTACTGGCATTGTCCTTGGAGGTTACTTGGCACTAATGACAGTAGTGTTCTTCTGGCTTATGAATGATACCGACTTCTTCTCGGAAAAGTTTCATGTGAGATCTTTGAGGGATAGGCCTGAGCAAATGATGGCGGCGTTGTACTTACAAGTGAGTATTGTGAGCCAAGCCCTTATTTTCGTCACAAGGTCACGTAGCTGGTCTTTTGTTGAACGCCCTGGACTTCTCTTACTTGGAGCTTTCATGATTGCTCAACTGGTTGCGACTCTCATAGCAGTCTATGCAAACTGGGGTTTTGCACGGATAAAGGGAGCTGGCTGGGGGTGGGCTGGTGTCATCTGGCTTTACAGTATAGTGACATATGTCCCTCTTGACTTTCTTAAATTCGCAATCCGATACATTCAAAGTGGAAAAGCGTGGAATAATCTTTTGGAGAACAAGACTGCCTTCACTACAAAGAAAGATTATGGAAAAGAGGAGAGAGAAGCACAGTGGGCTGCAGCACAGAGGACCCTTCATGGTCTTCAGCCACCTGAAACAAACAACCTTTTCAATGAGAGGAACAGTTACAGGGAGCTTTCCGAGATTGCAGAACAAGCCAAGCGGAGGGCTGAGGTTGCAAGGCTTCGTGAGTTGACTACTCTGAAGGGCCATGTTGAATCAGTTGTGAAGCTCAAAGGGCTAGACATCGATACAATTCAACAACATTATACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

955

Amino Acids

105.03

Weight (kDa)

7.24

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 21 - 84 6.3e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 134 - 232 1.4e-30 P-type ATPase actuator domain
Hydrolase PF00702 328 - 605 5.2e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 376 - 496 8.3e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 990
AccB1I GGYRCC 2 cut(s) 460, 1286
AccI GTMKAC 1 cut(s) 1224
AccII CGCG 1 cut(s) 1275
AciI CCGC 5 cut(s) 514, 1620, 1646, 2279, 2749
AclWI GGATC 5 cut(s) 524, 1160, 1469, 1560, 2100
AcoI YGGCCR 4 cut(s) 3, 390, 1630, 1647
AcsI RAATTY 1 cut(s) 2528
AcuI CTGAAG 4 cut(s) 1845, 2153, 2654, 2810
AcyI GRCGYC 1 cut(s) 987
AfaI GTAC 7 cut(s) 100, 803, 824, 1410, 1610, 1911, 2288
AfiI CCNNNNNNNGG 6 cut(s) 523, 561, 562, 1639, 1666, 2751
AflIII ACRYGT 2 cut(s) 617, 1605
AjnI CCWGG 5 cut(s) 463, 1432, 1481, 1882, 2362
AjuI GAANNNNNNNTTGG 2 cut(s) 2558, 2590
AleI CACNNNNGTG 1 cut(s) 1761
AloI GAACNNNNNNTCC 2 cut(s) 483, 515
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 1 cut(s) 462
Alw44I GTGCAC 1 cut(s) 662
AlwI GGATC 5 cut(s) 524, 1160, 1469, 1560, 2100
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 7 cut(s) 3, 15, 390, 1630, 1647, 2261, 2794
ApaLI GTGCAC 1 cut(s) 662
ApoI RAATTY 1 cut(s) 2528
ArsI GACNNNNNNTTYG 2 cut(s) 997, 1029
Asp700I GAANNNNTTC 3 cut(s) 63, 1503, 2235
AspLEI GCGC 2 cut(s) 819, 1277
AspS9I GGNCC 3 cut(s) 152, 2655, 2794
AsuHPI GGTGA 5 cut(s) 539, 641, 892, 937, 1363
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 2 cut(s) 152, 2655
AxyI CCTNAGG 1 cut(s) 1170
BaeGI GKGCMC 3 cut(s) 666, 720, 1291
BalI TGGCCA 1 cut(s) 1632
BanI GGYRCC 2 cut(s) 460, 1286
BanII GRGCYC 1 cut(s) 1516
BarI GAAGNNNNNNTAC 2 cut(s) 806, 838
BauI CACGAG 1 cut(s) 1179
BbsI GAAGAC 1 cut(s) 2660
Bbv12I GWGCWC 1 cut(s) 666
BbvCI CCTCAGC 1 cut(s) 2757
BceAI ACGGC 2 cut(s) 1450, 1811
BciT130I CCWGG 5 cut(s) 465, 1434, 1483, 1884, 2364
BciVI GTATCC 1 cut(s) 771
BclI TGATCA 3 cut(s) 544, 1896, 2046
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 5 cut(s) 164, 398, 1113, 1278, 2828
BfmI CTRYAG 4 cut(s) 378, 994, 1221, 2643
BfuI GTATCC 1 cut(s) 771
BglI GCCNNNNNGGC 1 cut(s) 389
BglII AGATCT 2 cut(s) 2139, 2246
BmcAI AGTACT 1 cut(s) 1911
Bme1390I CCNGG 5 cut(s) 465, 1434, 1483, 1884, 2364
Bme18I GGWCC 2 cut(s) 152, 2655
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 3 cut(s) 152, 2655, 2794
BmiI GGNNCC 5 cut(s) 130, 462, 916, 1288, 2657
BmrFI CCNGG 5 cut(s) 465, 1434, 1483, 1884, 2364
BmrI ACTGGG 3 cut(s) 570, 1017, 2443
BmsI GCATC 8 cut(s) 436, 499, 1120, 1129, 1279, 1633, 1825, 1834
BmuI ACTGGG 3 cut(s) 570, 1017, 2443
BoxI GACNNNNGTC 1 cut(s) 2507
BpiI GAAGAC 1 cut(s) 2660
BplI GAGNNNNNCTC 2 cut(s) 1222, 1254
BpmI CTGGAG 4 cut(s) 138, 486, 1722, 1794
Bpu10I CCTNAGC 3 cut(s) 1293, 2265, 2757
BpuEI CTTGAG 1 cut(s) 176
Bsa29I ATCGAT 2 cut(s) 1251, 2835
BsaAI YACGTR 2 cut(s) 1979, 2339
BsaBI GATNNNNATC 1 cut(s) 1044
BsaHI GRCGYC 1 cut(s) 987
BsaJI CCNNGG 9 cut(s) 261, 903, 1065, 1433, 1442, 1481, 1883, 2161, 2362
BsaWI WCCGGW 1 cut(s) 67
BsaXI ACNNNNNCTCC 6 cut(s) 483, 513, 1576, 1606, 1767, 1797
Bsc4I CCNNNNNNNGG 6 cut(s) 523, 561, 562, 1639, 1666, 2751
Bse118I RCCGGY 2 cut(s) 5, 392
Bse1I ACTGG 9 cut(s) 121, 565, 661, 1012, 1582, 1777, 2155, 2406, 2438
Bse21I CCTNAGG 1 cut(s) 1170
Bse3DI GCAATG 1 cut(s) 1622
Bse8I GATNNNNATC 1 cut(s) 1044
BseBI CCWGG 5 cut(s) 465, 1434, 1483, 1884, 2364
BseCI ATCGAT 2 cut(s) 1251, 2835
BseDI CCNNGG 9 cut(s) 261, 903, 1065, 1433, 1442, 1481, 1883, 2161, 2362
BseGI GGATG 5 cut(s) 601, 1135, 1648, 1944, 2055
BseJI GATNNNNATC 1 cut(s) 1044
BseLI CCNNNNNNNGG 6 cut(s) 523, 561, 562, 1639, 1666, 2751
BseMI GCAATG 1 cut(s) 1622
BseMII CTCAG 5 cut(s) 429, 1047, 1284, 2256, 2748
BseNI ACTGG 9 cut(s) 121, 565, 661, 1012, 1582, 1777, 2155, 2406, 2438
BseRI GAGGAG 1 cut(s) 2636
BseSI GKGCMC 3 cut(s) 666, 720, 1291
BseYI CCCAGC 1 cut(s) 2463
BsgI GTGCAG 3 cut(s) 669, 1515, 1926
Bsh1236I CGCG 1 cut(s) 1275
BshFI GGCC 7 cut(s) 5, 17, 392, 1632, 1649, 2263, 2796
BshNI GGYRCC 2 cut(s) 460, 1286
BshVI ATCGAT 2 cut(s) 1251, 2835
BsiHKAI GWGCWC 1 cut(s) 666
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 3 cut(s) 6, 68, 393
BslFI GGGAC 4 cut(s) 553, 1024, 1450, 2495
BslI CCNNNNNNNGG 6 cut(s) 523, 561, 562, 1639, 1666, 2751
BsmAI GTCTC 1 cut(s) 462
BsmFI GGGAC 4 cut(s) 553, 1024, 1450, 2495
BsmI GAATGC 1 cut(s) 1161
BsnI GGCC 7 cut(s) 5, 17, 392, 1632, 1649, 2263, 2796
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 4 cut(s) 666, 720, 1291, 1516
Bsp1407I TGTACA 1 cut(s) 98
Bsp19I CCATGG 2 cut(s) 903, 1442
BspACI CCGC 5 cut(s) 514, 1620, 1646, 2279, 2749
BspANI GGCC 7 cut(s) 5, 17, 392, 1632, 1649, 2263, 2796
BspCNI CTCAG 5 cut(s) 430, 1046, 1285, 2257, 2749
BspDI ATCGAT 2 cut(s) 1251, 2835
BspFNI CGCG 1 cut(s) 1275
BspHI TCATGA 2 cut(s) 2076, 2388
BspLI GGNNCC 5 cut(s) 130, 462, 916, 1288, 2657
BspMAI CTGCAG 3 cut(s) 382, 998, 2647
BspPI GGATC 5 cut(s) 524, 1160, 1469, 1560, 2100
BspT107I GGYRCC 2 cut(s) 460, 1286
BsrDI GCAATG 1 cut(s) 1622
BsrFI RCCGGY 2 cut(s) 5, 392
BsrGI TGTACA 1 cut(s) 98
BsrI ACTGG 9 cut(s) 121, 565, 661, 1012, 1582, 1777, 2155, 2406, 2438
BssAI RCCGGY 2 cut(s) 5, 392
BssECI CCNNGG 9 cut(s) 261, 903, 1065, 1433, 1442, 1481, 1883, 2161, 2362
BssNI GRCGYC 1 cut(s) 987
BssSI CACGAG 1 cut(s) 1179
BssT1I CCWWGG 5 cut(s) 261, 903, 1065, 1442, 2161
Bst2BI CACGAG 1 cut(s) 1179
Bst2UI CCWGG 5 cut(s) 465, 1434, 1483, 1884, 2364
Bst4CI ACNGT 7 cut(s) 889, 1493, 2188, 2495, 2637, 2711, 2860
Bst6I CTCTTC 2 cut(s) 24, 162
BstACI GRCGYC 1 cut(s) 987
BstAPI GCANNNNNTGC 3 cut(s) 1090, 1856, 2426
BstAUI TGTACA 1 cut(s) 98
BstBAI YACGTR 2 cut(s) 1979, 2339
BstC8I GCNNGC 8 cut(s) 7, 133, 137, 514, 979, 1161, 1560, 2461
BstDSI CCRYGG 2 cut(s) 903, 1442
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 5 cut(s) 601, 1135, 1648, 1944, 2055
BstFNI CGCG 1 cut(s) 1275
BstHHI GCGC 2 cut(s) 819, 1277
BstMAI GTCTC 1 cut(s) 462
BstNI CCWGG 5 cut(s) 465, 1434, 1483, 1884, 2364
BstNSI RCATGY 3 cut(s) 621, 1088, 1609
BstPAI GACNNNNGTC 1 cut(s) 2507
BstSCI CCNGG 5 cut(s) 463, 1432, 1481, 1882, 2362
BstSFI CTRYAG 4 cut(s) 378, 994, 1221, 2643
BstSLI GKGCMC 3 cut(s) 666, 720, 1291
BstSNI TACGTA 1 cut(s) 1979
BstUI CGCG 1 cut(s) 1275
BstV2I GAAGAC 1 cut(s) 2660
BstX2I RGATCY 2 cut(s) 2139, 2246
BstXI CCANNNNNNTGG 2 cut(s) 1072, 2124
BstYI RGATCY 2 cut(s) 2139, 2246
Bsu15I ATCGAT 2 cut(s) 1251, 2835
Bsu36I CCTNAGG 1 cut(s) 1170
BsuI GTATCC 1 cut(s) 771
BsuRI GGCC 7 cut(s) 5, 17, 392, 1632, 1649, 2263, 2796
BsuTUI ATCGAT 2 cut(s) 1251, 2835
BtgI CCRYGG 2 cut(s) 903, 1442
BtgZI GCGATG 2 cut(s) 1256, 2035
BtsCI GGATG 5 cut(s) 601, 1135, 1648, 1944, 2055
BtsI GCAGTG 2 cut(s) 735, 1003
BtsIMutI CAGTG 5 cut(s) 735, 1003, 1498, 1875, 2642
Cac8I GCNNGC 8 cut(s) 7, 133, 137, 514, 979, 1161, 1560, 2461
CciI TCATGA 2 cut(s) 2076, 2388
CfoI GCGC 2 cut(s) 819, 1277
Cfr10I RCCGGY 2 cut(s) 5, 392
Cfr13I GGNCC 3 cut(s) 152, 2655, 2794
ClaI ATCGAT 2 cut(s) 1251, 2835
CseI GACGC 1 cut(s) 1799
Csp6I GTAC 7 cut(s) 99, 802, 823, 1409, 1609, 1910, 2287
CspCI CAANNNNNGTGG 2 cut(s) 753, 788
CviQI GTAC 7 cut(s) 99, 802, 823, 1409, 1609, 1910, 2287
EaeI YGGCCR 4 cut(s) 3, 390, 1630, 1647
Eam1104I CTCTTC 2 cut(s) 24, 162
EarI CTCTTC 2 cut(s) 24, 162
Eco105I TACGTA 1 cut(s) 1979
Eco130I CCWWGG 5 cut(s) 261, 903, 1065, 1442, 2161
Eco147I AGGCCT 1 cut(s) 2263
Eco24I GRGCYC 1 cut(s) 1516
Eco47I GGWCC 2 cut(s) 152, 2655
Eco57I CTGAAG 4 cut(s) 1845, 2153, 2654, 2810
Eco81I CCTNAGG 1 cut(s) 1170
Eco88I CYCGRG 1 cut(s) 521
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 2655
EcoRII CCWGG 5 cut(s) 463, 1432, 1481, 1882, 2362
EcoT14I CCWWGG 5 cut(s) 261, 903, 1065, 1442, 2161
EcoT38I GRGCYC 1 cut(s) 1516
ErhI CCWWGG 5 cut(s) 261, 903, 1065, 1442, 2161
FalI AAGNNNNNCTT 8 cut(s) 1843, 1875, 2009, 2041, 2126, 2158, 2576, 2608
FaqI GGGAC 4 cut(s) 553, 1024, 1450, 2495
FauI CCCGC 1 cut(s) 521
FauNDI CATATG 1 cut(s) 2506
FbaI TGATCA 3 cut(s) 544, 1896, 2046
FblI GTMKAC 1 cut(s) 1224
FokI GGATG 5 cut(s) 608, 1142, 1655, 1951, 2042
FriOI GRGCYC 1 cut(s) 1516
FspBI CTAG 5 cut(s) 164, 398, 1113, 1278, 2828
GlaI GCGC 2 cut(s) 818, 1276
GsaI CCCAGC 1 cut(s) 2467
GsuI CTGGAG 4 cut(s) 138, 486, 1722, 1794
HaeIII GGCC 7 cut(s) 5, 17, 392, 1632, 1649, 2263, 2796
HapII CCGG 3 cut(s) 6, 68, 393
HgaI GACGC 1 cut(s) 1799
HhaI GCGC 2 cut(s) 819, 1277
Hin1I GRCGYC 1 cut(s) 987
Hin6I GCGC 2 cut(s) 817, 1275
HinP1I GCGC 2 cut(s) 817, 1275
HincII GTYRAC 1 cut(s) 2781
HindII GTYRAC 1 cut(s) 2781
HpaII CCGG 3 cut(s) 6, 68, 393
HphI GGTGA 5 cut(s) 539, 641, 892, 937, 1363
Hpy166II GTNNAC 7 cut(s) 623, 664, 697, 1225, 1376, 1669, 2781
Hpy8I GTNNAC 7 cut(s) 623, 664, 697, 1225, 1376, 1669, 2781
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 7 cut(s) 889, 1493, 2188, 2495, 2637, 2711, 2860
HpyCH4IV ACGT 4 cut(s) 987, 1372, 1978, 2338
HpySE526I ACGT 4 cut(s) 987, 1372, 1978, 2338
Hsp92I GRCGYC 1 cut(s) 987
HspAI GCGC 2 cut(s) 817, 1275
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Ksp22I TGATCA 3 cut(s) 544, 1896, 2046
LmnI GCTCC 8 cut(s) 128, 406, 920, 938, 1798, 2381, 2456, 2719
LweI GCATC 8 cut(s) 436, 499, 1120, 1129, 1279, 1633, 1825, 1834
MaeI CTAG 5 cut(s) 164, 398, 1113, 1278, 2828
MaeII ACGT 4 cut(s) 987, 1372, 1978, 2338
MaeIII GTNAC 7 cut(s) 577, 898, 2168, 2326, 2334, 2500, 2711
MfeI CAATTG 3 cut(s) 765, 873, 1146
MflI RGATCY 2 cut(s) 2139, 2246
MhlI GDGCHC 4 cut(s) 666, 720, 1291, 1516
MlsI TGGCCA 1 cut(s) 1632
MluNI TGGCCA 1 cut(s) 1632
MlyI GAGTC 4 cut(s) 53, 575, 1438, 2404
Mox20I TGGCCA 1 cut(s) 1632
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 3 cut(s) 63, 1503, 2235
MscI TGGCCA 1 cut(s) 1632
MseI TTAA 4 cut(s) 330, 1304, 2000, 2526
MslI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
Msp20I TGGCCA 1 cut(s) 1632
MspA1I CMGCKG 2 cut(s) 91, 2124
MspI CCGG 3 cut(s) 6, 68, 393
MspR9I CCNGG 5 cut(s) 465, 1434, 1483, 1884, 2364
MunI CAATTG 3 cut(s) 765, 873, 1146
Mva1269I GAATGC 1 cut(s) 1161
MvaI CCWGG 5 cut(s) 465, 1434, 1483, 1884, 2364
MvnI CGCG 1 cut(s) 1275
NaeI GCCGGC 1 cut(s) 7
NcoI CCATGG 2 cut(s) 903, 1442
NdeI CATATG 1 cut(s) 2506
NgoMIV GCCGGC 1 cut(s) 5
NlaIV GGNNCC 5 cut(s) 130, 462, 916, 1288, 2657
NmuCI GTSAC 4 cut(s) 898, 2326, 2334, 2500
NspI RCATGY 3 cut(s) 621, 1088, 1609
OliI CACNNNNGTG 1 cut(s) 1761
PaeR7I CTCGAG 1 cut(s) 521
PagI TCATGA 2 cut(s) 2076, 2388
PceI AGGCCT 1 cut(s) 2263
PciI ACATGT 2 cut(s) 617, 1605
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 1378
PctI GAATGC 1 cut(s) 1161
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 3 cut(s) 63, 1503, 2235
PfeI GAWTC 6 cut(s) 64, 214, 586, 867, 1253, 2804
PleI GAGTC 4 cut(s) 52, 574, 1437, 2404
PpsI GAGTC 4 cut(s) 52, 574, 1437, 2404
Ppu21I YACGTR 2 cut(s) 1979, 2339
PpuMI RGGWCCY 1 cut(s) 2655
PscI ACATGT 2 cut(s) 617, 1605
PshAI GACNNNNGTC 1 cut(s) 2507
Psp5II RGGWCCY 1 cut(s) 2655
Psp6I CCWGG 5 cut(s) 463, 1432, 1481, 1882, 2362
PspFI CCCAGC 1 cut(s) 2463
PspGI CCWGG 5 cut(s) 463, 1432, 1481, 1882, 2362
PspN4I GGNNCC 5 cut(s) 130, 462, 916, 1288, 2657
PspPI GGNCC 3 cut(s) 152, 2655, 2794
PspPPI RGGWCCY 1 cut(s) 2655
PstI CTGCAG 3 cut(s) 382, 998, 2647
PsuI RGATCY 2 cut(s) 2139, 2246
PvuII CAGCTG 2 cut(s) 91, 2124
RsaI GTAC 7 cut(s) 100, 803, 824, 1410, 1610, 1911, 2288
RsaNI GTAC 7 cut(s) 99, 802, 823, 1409, 1609, 1910, 2287
RseI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
SaqAI TTAA 4 cut(s) 330, 1304, 2000, 2526
Sau96I GGNCC 3 cut(s) 152, 2655, 2794
ScaI AGTACT 1 cut(s) 1911
SchI GAGTC 4 cut(s) 53, 575, 1438, 2404
ScrFI CCNGG 5 cut(s) 465, 1434, 1483, 1884, 2364
SduI GDGCHC 4 cut(s) 666, 720, 1291, 1516
SfaNI GCATC 8 cut(s) 436, 499, 1120, 1129, 1279, 1633, 1825, 1834
SfcI CTRYAG 4 cut(s) 378, 994, 1221, 2643
Sfr274I CTCGAG 1 cut(s) 521
SinI GGWCC 2 cut(s) 152, 2655
SlaI CTCGAG 1 cut(s) 521
SmiMI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
SmlI CTYRAG 2 cut(s) 191, 521
SmoI CTYRAG 2 cut(s) 191, 521
SnaBI TACGTA 1 cut(s) 1979
SseBI AGGCCT 1 cut(s) 2263
SsiI CCGC 5 cut(s) 514, 1620, 1646, 2279, 2749
SspMI CTAG 5 cut(s) 164, 398, 1113, 1278, 2828
StuI AGGCCT 1 cut(s) 2263
StyD4I CCNGG 5 cut(s) 463, 1432, 1481, 1882, 2362
StyI CCWWGG 5 cut(s) 261, 903, 1065, 1442, 2161
TaaI ACNGT 7 cut(s) 889, 1493, 2188, 2495, 2637, 2711, 2860
TaiI ACGT 4 cut(s) 990, 1375, 1981, 2341
TaqI TCGA 8 cut(s) 27, 51, 351, 430, 522, 1251, 2095, 2835
TatI WGTACW 4 cut(s) 98, 822, 1909, 2286
TauI GCSGC 2 cut(s) 1649, 2282
TfiI GAWTC 6 cut(s) 64, 214, 586, 867, 1253, 2804
Tru1I TTAA 4 cut(s) 330, 1304, 2000, 2526
Tru9I TTAA 4 cut(s) 330, 1304, 2000, 2526
TscAI CASTG 5 cut(s) 742, 1003, 1498, 1882, 2642
TseFI GTSAC 4 cut(s) 898, 2326, 2334, 2500
Tsp45I GTSAC 4 cut(s) 898, 2326, 2334, 2500
TspDTI ATGAA 9 cut(s) 337, 612, 1740, 1955, 2026, 2222, 2228, 2377, 2651
TspGWI ACGGA 4 cut(s) 37, 76, 2082, 2461
TspRI CASTG 5 cut(s) 742, 1003, 1498, 1882, 2642
VneI GTGCAC 1 cut(s) 662
VpaK11BI GGWCC 2 cut(s) 152, 2655
XagI CCTNNNNNAGG 1 cut(s) 521
XapI RAATTY 1 cut(s) 2528
XceI RCATGY 3 cut(s) 621, 1088, 1609
XcmI CCANNNNNNNNNTGG 1 cut(s) 970
XhoI CTCGAG 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 1224
XmnI GAANNNNTTC 3 cut(s) 63, 1503, 2235
XspI CTAG 5 cut(s) 164, 398, 1113, 1278, 2828
ZraI GACGTC 1 cut(s) 988
ZrmI AGTACT 1 cut(s) 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.