MD15G1108400.v1.1

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006441
Physical Location & Seq
Reverse (-)
7555975 .. 7563559
7585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1108400.v1.1.491

Sequence Viewer

Length: 3099 bp
ATGGGCCCACTCGTGGATCCCCACCTACTCCCAAACTCAGGTGTATATAAATCAAGCCAGTGTGGACTCCCTAGCTCTCCTTCATTCATTCACTCACTCCCACCATTTCACCCGCCCCCGCCGCGGGCTCCGAGACCCGAAAACCCGATTCCCCCATTCCCTCCTCCCCTCATCCTCTCTAACCTTTTCATGGTAGCTCTGACCTCCTCCGAGCTCCCCAGCGACTCCTCCACCATGGGCAGCGATAAGGCCATCAGCCTCGAAGAGATAAAAAACGAGTCCGTCGATCTGGAACGGATTCCGATAGAGGAAGTCTTTGAGCAGCTGAAATGTACAAGAGAAGGTCTGACTGCAGACGAAGGAGCCAACAGGCTTCAAGTCTTTGGACCAAACAAATTAGAAGAGAAAAAGGAGAGCAAACTGCTCAAGTTCTTGGGATTTATGTGGAATCCTTTGTCATGGGTTATGGAAGCTGCTGCTTTGATGGCTATTGTCTTGGCAAATGGTGATGGAAGGCCTCCTGATTGGCAGGACTTTGTTGGCATTGTTGTTCTTCTGGTCATTAACTCTACTATCAGTTTCATCGAAGAAAACAATGCTGGTAATGCTGCTGCAGCCCTTATGGCTGGTCTAGCTCCCAAGACTAAGGTTCTTAGAGATGGTCGATGGACTGAGCAAGATGCTTCAATTTTGGTGCCTGGAGACATAATCAGTATCAAATTGGGAGATATTGTTCCTGCTGATGCCCGCCTTCTCGAGGGTGATCCTCTGAAAATTGATCAATCTGCCCTTACTGGCGAGTCCCTTCCTGTTACTAAGAATCCTTCAGATGAAGTGTTTTCTGGCTCAACATGTAAACAGGGTGAAATTGAAGCAGTTGTTATTGCTACTGGTGTGCACACCTTCTTCGGTAAGGCCGCACATCTGGTGGACAGTACCAATCAAGTTGGGCACTTCCAGAAAGTTCTCACTGCCATTGGGAACTTCTGCATTTGTTCAATTGCCCTGGGGATACTAATTGAGATAATAGTAATGTACCCAATACAGAGGCGCAAGTACAGGCAAGGGATTGATAATTTGCTGGTTCTCTTGATTGGAGGAATCCCAATTGCCATGCCAACTGTTTTATCTGTCACTATGGCTATTGGCTCCCACAGGCTTTCTCAGCAAGGAGCTATCACCAAGAGAATGACTGCCATTGAGGAAATGGCTGGCATGGACGTCCTCTGCAGTGATAAGACTGGGACTTTGACCCTGAACAAGCTCACAGTTGATAGAAATCTGATTGAAGTGTTTGCCAAGGGTGTGGAGAAAGAGCATGTTATGCTTCTTGCAGCAAGGGCTTCTAGGACTGAAAATCAGGATGCTATTGATGCTGCAATTGTAGGAATGCTTGCTGATCCTAAGGAGGCACGAGCTGGTATAAGAGAAATCCATTTCCTACCATTCAACCCCGTAGACAAGAGAACTGCTCTGACCTACATCGATTCCGATGGAAATTGGCATCGGGCTAGCAAAGGTGCCCCTGAGCAGATATTAACCCTTTGCAACTGCAAGGAAGATTTCAAGAAAAGGGTGCATGCGGTGATTGATAAGTTTGCTGAACGTGGACTTCGTTCTCTAGCTGTTGCAAGACAGCAAGTACCAGAGAAAACAAAAGAGAGTCAAGGGACACCATGGCAGTTTGTTGGTTTATTGCCGTTATTTGATCCTCCTAGGCATGACAGTGCAGAAACAATTCGCAGAGCTCTCAATCTCGGTGTGAATGTGAAAATGATTACAGGGGACCAGCTTGCCATTGGCAAGGAAACTGGAAGGAGGCTTGGTATGGGAACAAACATGTACCCGTCTTCCGCATTACTTGGCCAAGACAAGGATGCAGCCATAGCTTCCCTCCCTGTGGACGAGTTGATTGAGAAGGCTGATGGATTTGCTGGAGTGTTTCCTGAACACAAATATGAAATTGTAAAGAGGCTGCAAGAGAGGAAGCACATCTGTGGAATGACTGGAGATGGTGTCAATGACGCTCCTGCATTGAAAAAAGCAGATATTGGTATTGCCGTAGCTGATGCTACTGATGCTGCAAGAAGTGCTTCAGATATTGTTCTCACTGAACCAGGATTAAGTGTGATCATAAGCGCAGTACTGACAAGCAGAGCAATATTCCAGAGGATGAAGAACTATACTATCTATGCTGTTTCAATTACCATACGTATAGTGTTTGGATTTATGTTCATTGCTTTGATATGGAAGTTTGACTTTGCACCCTTCATGGTATTGATCATTGCCATCCTAAATGACGGAACCATCATGACAATATCAAAGGATCGAGTGAAGCCATCTCCACTGCCAGACAGCTGGAAACTGAGGGAGATCTTTGCTACTGGTATTGTCCTTGGAGGTTACATGGCACTAATGACTGTAGTCTTCTTCTGGCTTATGAGAGATACCGACTTCTTCTCGGATAAGTTTCATGTGAGATCTTTGAGAAATAGGCCTGAGCAAATGATGGCAGCGTTGTACTTACAAGTGAGTATTGTGAGCCAAGCCCTTATTTTCGTCACAAGGTCTCGCAGCTGGTCGTTTGTTGAACGCCCTGGACTTCTCTTACTTGGAGCTTTCATGATTGCTCAACTGGTTGCAACTCTCATAGCAGTGTATGCAAACTGGGGTTTTGCACGGATAGAGGGAGCTGGCTGGGGGTGGGCTGGTGTCATCTGGATTTACAGTATAGTGACATATGTCCCTCTTGACTTGCTTAAATTTGCAATCCGATACATTCAAAGCGGGAAAGCGTGGAATAATCTTTTGGAGAACAAGACTGCCTTCACTACAAAGAAAGATTATGGAAAAGAGGAAAGAGAAGCACAGTGGGCTGCAGCACAGAGGACCCTTCATGGTCTTCAGCCACCTGAGACGAACAACCTTTTCAATGAGAAGAACAGTTACAGGGAACTTTCCGAGATTGCAGAACAAGCCAAGCGGAGGGCTGAGGTTGCAAGGCTTCGGGAGTTGCATACTCTGAAGGGTCACGTCGAGTCAGTTGTGAAGCTCAAAGGACTCGACATCGATACAATCCAACAACATTATACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1033

Amino Acids

113.75

Weight (kDa)

6.86

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 99 - 162 7e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 212 - 310 1.5e-30 P-type ATPase actuator domain
Hydrolase PF00702 406 - 683 4.1e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 455 - 574 6.5e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1224
AccB1I GGYRCC 2 cut(s) 694, 1520
AccI GTMKAC 1 cut(s) 1458
AccII CGCG 1 cut(s) 124
AclWI GGATC 6 cut(s) 11, 24, 758, 1394, 1703, 2334
AcoI YGGCCR 1 cut(s) 1864
AcsI RAATTY 1 cut(s) 2762
AcuI CTGAAG 4 cut(s) 810, 2079, 2888, 3044
AcyI GRCGYC 1 cut(s) 1221
AfaI GTAC 8 cut(s) 334, 937, 1037, 1058, 1644, 1844, 2145, 2522
AfiI CCNNNNNNNGG 9 cut(s) 13, 38, 123, 124, 190, 757, 1873, 1900, 2985
AflIII ACRYGT 2 cut(s) 851, 1839
AjiI CACGTC 1 cut(s) 3034
AjnI CCWGG 4 cut(s) 697, 1005, 2116, 2596
AjuI GAANNNNNNNTTGG 2 cut(s) 2792, 2824
AleI CACNNNNGTG 1 cut(s) 1995
AloI GAACNNNNNNTCC 2 cut(s) 717, 749
Alw21I GWGCWC 3 cut(s) 216, 900, 1750
Alw26I GTCTC 4 cut(s) 127, 696, 2574, 2909
Alw44I GTGCAC 1 cut(s) 896
AlwI GGATC 6 cut(s) 11, 24, 758, 1394, 1703, 2334
Ama87I CYCGRG 1 cut(s) 755
AoxI GGCC 6 cut(s) 4, 249, 515, 915, 1864, 2495
ApaI GGGCCC 1 cut(s) 8
ApaLI GTGCAC 1 cut(s) 896
ApoI RAATTY 1 cut(s) 2762
ArsI GACNNNNNNTTYG 2 cut(s) 1231, 1263
Asp700I GAANNNNTTC 3 cut(s) 297, 1737, 2092
AspA2I CCTAGG 1 cut(s) 1715
AspLEI GCGC 2 cut(s) 1053, 2141
AspS9I GGNCC 5 cut(s) 4, 5, 386, 1786, 2889
AsuHPI GGTGA 6 cut(s) 101, 518, 773, 875, 1171, 1597
AsuNHI GCTAGC 1 cut(s) 1511
AvaI CYCGRG 1 cut(s) 755
AvaII GGWCC 3 cut(s) 386, 1786, 2889
AvrII CCTAGG 1 cut(s) 1715
AxyI CCTNAGG 1 cut(s) 1404
BaeGI GKGCMC 4 cut(s) 8, 900, 954, 1525
BalI TGGCCA 1 cut(s) 1866
BamHI GGATCC 1 cut(s) 16
BanI GGYRCC 2 cut(s) 694, 1520
BanII GRGCYC 4 cut(s) 8, 130, 216, 1750
BarI GAAGNNNNNNTAC 2 cut(s) 2930, 2962
BauI CACGAG 2 cut(s) 11, 1413
BbsI GAAGAC 3 cut(s) 1842, 2419, 2894
Bbv12I GWGCWC 3 cut(s) 216, 900, 1750
BbvCI CCTCAGC 1 cut(s) 2991
BceAI ACGGC 2 cut(s) 1684, 2045
BciT130I CCWGG 4 cut(s) 699, 1007, 2118, 2598
BciVI GTATCC 1 cut(s) 1005
BclI TGATCA 3 cut(s) 778, 2130, 2280
BcoDI GTCTC 4 cut(s) 127, 696, 2574, 2909
BfaI CTAG 6 cut(s) 72, 632, 1347, 1512, 1622, 1716
BfmI CTRYAG 5 cut(s) 351, 612, 1228, 2421, 2877
BfuI GTATCC 1 cut(s) 1005
BglI GCCNNNNNGGC 1 cut(s) 623
BglII AGATCT 2 cut(s) 2373, 2480
BlnI CCTAGG 1 cut(s) 1715
BmcAI AGTACT 1 cut(s) 2145
Bme1390I CCNGG 4 cut(s) 699, 1007, 2118, 2598
Bme18I GGWCC 3 cut(s) 386, 1786, 2889
BmeT110I CYCGRG 1 cut(s) 755
BmgBI CACGTC 1 cut(s) 3034
BmgT120I GGNCC 5 cut(s) 4, 5, 386, 1786, 2889
BmrFI CCNGG 4 cut(s) 699, 1007, 2118, 2598
BmrI ACTGGG 2 cut(s) 1251, 2677
BmsI GCATC 8 cut(s) 670, 733, 1354, 1363, 1513, 1867, 2059, 2068
BmtI GCTAGC 1 cut(s) 1515
BmuI ACTGGG 2 cut(s) 1251, 2677
BoxI GACNNNNGTC 2 cut(s) 2423, 2741
BpiI GAAGAC 3 cut(s) 1842, 2419, 2894
BplI GAGNNNNNCTC 2 cut(s) 1456, 1488
BpmI CTGGAG 3 cut(s) 720, 1956, 2028
Bpu10I CCTNAGC 3 cut(s) 1527, 2499, 2991
BpuEI CTTGAG 1 cut(s) 410
Bsa29I ATCGAT 2 cut(s) 1485, 3069
BsaAI YACGTR 1 cut(s) 2213
BsaBI GATNNNNATC 1 cut(s) 1278
BsaHI GRCGYC 1 cut(s) 1221
BsaI GGTCTC 2 cut(s) 127, 2574
BsaJI CCNNGG 9 cut(s) 122, 234, 1005, 1006, 1299, 1676, 1715, 2395, 2596
BsaXI ACNNNNNCTCC 6 cut(s) 717, 747, 1810, 1840, 2001, 2031
Bsc4I CCNNNNNNNGG 9 cut(s) 13, 38, 123, 124, 190, 757, 1873, 1900, 2985
Bse1I ACTGG 9 cut(s) 58, 799, 895, 1246, 1816, 2011, 2389, 2640, 2672
Bse21I CCTNAGG 1 cut(s) 1404
Bse3DI GCAATG 2 cut(s) 2235, 2283
Bse8I GATNNNNATC 1 cut(s) 1278
BseBI CCWGG 4 cut(s) 699, 1007, 2118, 2598
BseCI ATCGAT 2 cut(s) 1485, 3069
BseDI CCNNGG 9 cut(s) 122, 234, 1005, 1006, 1299, 1676, 1715, 2395, 2596
BseGI GGATG 5 cut(s) 171, 1369, 1882, 2178, 2289
BseJI GATNNNNATC 1 cut(s) 1278
BseLI CCNNNNNNNGG 9 cut(s) 13, 38, 123, 124, 190, 757, 1873, 1900, 2985
BseMI GCAATG 2 cut(s) 2235, 2283
BseMII CTCAG 8 cut(s) 51, 663, 1178, 1518, 2357, 2490, 2904, 2982
BseNI ACTGG 9 cut(s) 58, 799, 895, 1246, 1816, 2011, 2389, 2640, 2672
BseRI GAGGAG 3 cut(s) 153, 196, 217
BseSI GKGCMC 4 cut(s) 8, 900, 954, 1525
BseYI CCCAGC 2 cut(s) 218, 2697
BsgI GTGCAG 1 cut(s) 1749
Bsh1236I CGCG 1 cut(s) 124
BshFI GGCC 6 cut(s) 6, 251, 517, 917, 1866, 2497
BshNI GGYRCC 2 cut(s) 694, 1520
BshVI ATCGAT 2 cut(s) 1485, 3069
BsiHKAI GWGCWC 3 cut(s) 216, 900, 1750
BsiHKCI CYCGRG 1 cut(s) 755
BslFI GGGAC 5 cut(s) 787, 1258, 1684, 1799, 2729
BslI CCNNNNNNNGG 9 cut(s) 13, 38, 123, 124, 190, 757, 1873, 1900, 2985
BsmAI GTCTC 4 cut(s) 127, 696, 2574, 2909
BsmBI CGTCTC 1 cut(s) 2909
BsmFI GGGAC 5 cut(s) 787, 1258, 1684, 1799, 2729
BsmI GAATGC 1 cut(s) 1395
BsnI GGCC 6 cut(s) 6, 251, 517, 917, 1866, 2497
Bso31I GGTCTC 2 cut(s) 127, 2574
BsoBI CYCGRG 1 cut(s) 755
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 7 cut(s) 8, 130, 216, 900, 954, 1525, 1750
Bsp1407I TGTACA 1 cut(s) 332
Bsp19I CCATGG 2 cut(s) 234, 1676
BspANI GGCC 6 cut(s) 6, 251, 517, 917, 1866, 2497
BspCNI CTCAG 8 cut(s) 50, 664, 1177, 1519, 2358, 2491, 2905, 2983
BspDI ATCGAT 2 cut(s) 1485, 3069
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 2 cut(s) 2310, 2622
BspMAI CTGCAG 4 cut(s) 355, 616, 1232, 2881
BspOI GCTAGC 1 cut(s) 1515
BspPI GGATC 6 cut(s) 11, 24, 758, 1394, 1703, 2334
BspT107I GGYRCC 2 cut(s) 694, 1520
BspTNI GGTCTC 2 cut(s) 127, 2574
BsrDI GCAATG 2 cut(s) 2235, 2283
BsrGI TGTACA 1 cut(s) 332
BsrI ACTGG 9 cut(s) 58, 799, 895, 1246, 1816, 2011, 2389, 2640, 2672
BssECI CCNNGG 9 cut(s) 122, 234, 1005, 1006, 1299, 1676, 1715, 2395, 2596
BssNI GRCGYC 1 cut(s) 1221
BssSI CACGAG 2 cut(s) 11, 1413
BssT1I CCWWGG 5 cut(s) 234, 1299, 1676, 1715, 2395
Bst2BI CACGAG 2 cut(s) 11, 1413
Bst2UI CCWGG 4 cut(s) 699, 1007, 2118, 2598
Bst4CI ACNGT 9 cut(s) 935, 1123, 1270, 1727, 2422, 2729, 2871, 2945, 3094
Bst6I CTCTTC 2 cut(s) 258, 396
BstACI GRCGYC 1 cut(s) 1221
BstAPI GCANNNNNTGC 3 cut(s) 1324, 2090, 2660
BstAUI TGTACA 1 cut(s) 332
BstBAI YACGTR 1 cut(s) 2213
BstC8I GCNNGC 8 cut(s) 126, 748, 1213, 1395, 1513, 1581, 1794, 2695
BstDSI CCRYGG 3 cut(s) 122, 234, 1676
BstENI CCTNNNNNAGG 1 cut(s) 755
BstF5I GGATG 5 cut(s) 171, 1369, 1882, 2178, 2289
BstFNI CGCG 1 cut(s) 124
BstHHI GCGC 2 cut(s) 1053, 2141
BstMAI GTCTC 4 cut(s) 127, 696, 2574, 2909
BstNI CCWGG 4 cut(s) 699, 1007, 2118, 2598
BstNSI RCATGY 4 cut(s) 855, 1322, 1583, 1843
BstPAI GACNNNNGTC 2 cut(s) 2423, 2741
BstSCI CCNGG 4 cut(s) 697, 1005, 2116, 2596
BstSFI CTRYAG 5 cut(s) 351, 612, 1228, 2421, 2877
BstSLI GKGCMC 4 cut(s) 8, 900, 954, 1525
BstSNI TACGTA 1 cut(s) 2213
BstUI CGCG 1 cut(s) 124
BstV2I GAAGAC 3 cut(s) 1842, 2419, 2894
BstX2I RGATCY 3 cut(s) 16, 2373, 2480
BstXI CCANNNNNNTGG 2 cut(s) 1306, 2358
BstYI RGATCY 3 cut(s) 16, 2373, 2480
Bsu15I ATCGAT 2 cut(s) 1485, 3069
Bsu36I CCTNAGG 1 cut(s) 1404
BsuI GTATCC 1 cut(s) 1005
BsuRI GGCC 6 cut(s) 6, 251, 517, 917, 1866, 2497
BsuTUI ATCGAT 2 cut(s) 1485, 3069
BtgI CCRYGG 3 cut(s) 122, 234, 1676
BtrI CACGTC 1 cut(s) 3034
BtsCI GGATG 5 cut(s) 171, 1369, 1882, 2178, 2289
BtsI GCAGTG 4 cut(s) 969, 1237, 2345, 2661
BtsIMutI CAGTG 8 cut(s) 65, 969, 1237, 1732, 2109, 2345, 2661, 2876
Cac8I GCNNGC 8 cut(s) 126, 748, 1213, 1395, 1513, 1581, 1794, 2695
CciI TCATGA 2 cut(s) 2310, 2622
CfoI GCGC 2 cut(s) 1053, 2141
Cfr13I GGNCC 5 cut(s) 4, 5, 386, 1786, 2889
Cfr42I CCGCGG 1 cut(s) 125
ClaI ATCGAT 2 cut(s) 1485, 3069
CseI GACGC 1 cut(s) 2033
Csp6I GTAC 8 cut(s) 333, 936, 1036, 1057, 1643, 1843, 2144, 2521
CviQI GTAC 8 cut(s) 333, 936, 1036, 1057, 1643, 1843, 2144, 2521
EaeI YGGCCR 1 cut(s) 1864
Eam1104I CTCTTC 2 cut(s) 258, 396
EarI CTCTTC 2 cut(s) 258, 396
Ecl136II GAGCTC 2 cut(s) 214, 1748
Eco105I TACGTA 1 cut(s) 2213
Eco130I CCWWGG 5 cut(s) 234, 1299, 1676, 1715, 2395
Eco147I AGGCCT 2 cut(s) 517, 2497
Eco24I GRGCYC 4 cut(s) 8, 130, 216, 1750
Eco31I GGTCTC 2 cut(s) 127, 2574
Eco47I GGWCC 3 cut(s) 386, 1786, 2889
Eco53kI GAGCTC 2 cut(s) 214, 1748
Eco57I CTGAAG 4 cut(s) 810, 2079, 2888, 3044
Eco81I CCTNAGG 1 cut(s) 1404
Eco88I CYCGRG 1 cut(s) 755
EcoICRI GAGCTC 2 cut(s) 214, 1748
EcoNI CCTNNNNNAGG 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 2889
EcoRII CCWGG 4 cut(s) 697, 1005, 2116, 2596
EcoT14I CCWWGG 5 cut(s) 234, 1299, 1676, 1715, 2395
EcoT38I GRGCYC 4 cut(s) 8, 130, 216, 1750
ErhI CCWWGG 5 cut(s) 234, 1299, 1676, 1715, 2395
Esp3I CGTCTC 1 cut(s) 2909
FalI AAGNNNNNCTT 6 cut(s) 2077, 2109, 2243, 2275, 2810, 2842
FaqI GGGAC 5 cut(s) 787, 1258, 1684, 1799, 2729
FauI CCCGC 5 cut(s) 117, 120, 126, 755, 2780
FauNDI CATATG 1 cut(s) 2740
FbaI TGATCA 3 cut(s) 778, 2130, 2280
FblI GTMKAC 1 cut(s) 1458
FokI GGATG 5 cut(s) 158, 1376, 1889, 2185, 2276
FriOI GRGCYC 4 cut(s) 8, 130, 216, 1750
FspBI CTAG 6 cut(s) 72, 632, 1347, 1512, 1622, 1716
GlaI GCGC 2 cut(s) 1052, 2140
GsaI CCCAGC 2 cut(s) 222, 2701
GsuI CTGGAG 3 cut(s) 720, 1956, 2028
HaeIII GGCC 6 cut(s) 6, 251, 517, 917, 1866, 2497
HgaI GACGC 1 cut(s) 2033
HhaI GCGC 2 cut(s) 1053, 2141
Hin1I GRCGYC 1 cut(s) 1221
Hin6I GCGC 2 cut(s) 1051, 2139
HinP1I GCGC 2 cut(s) 1051, 2139
HphI GGTGA 6 cut(s) 101, 518, 773, 875, 1171, 1597
Hpy166II GTNNAC 7 cut(s) 65, 857, 898, 931, 1459, 1610, 1903
Hpy8I GTNNAC 7 cut(s) 65, 857, 898, 931, 1459, 1610, 1903
Hpy99I CGWCG 2 cut(s) 287, 3038
HpyCH4III ACNGT 9 cut(s) 935, 1123, 1270, 1727, 2422, 2729, 2871, 2945, 3094
HpyCH4IV ACGT 4 cut(s) 1221, 1606, 2212, 3033
HpySE526I ACGT 4 cut(s) 1221, 1606, 2212, 3033
Hsp92I GRCGYC 1 cut(s) 1221
HspAI GCGC 2 cut(s) 1051, 2139
Ksp22I TGATCA 3 cut(s) 778, 2130, 2280
KspI CCGCGG 1 cut(s) 125
LmnI GCTCC 9 cut(s) 133, 219, 362, 640, 1154, 1172, 2032, 2615, 2690
LweI GCATC 8 cut(s) 670, 733, 1354, 1363, 1513, 1867, 2059, 2068
MaeI CTAG 6 cut(s) 72, 632, 1347, 1512, 1622, 1716
MaeII ACGT 4 cut(s) 1221, 1606, 2212, 3033
MaeIII GTNAC 7 cut(s) 811, 1132, 2402, 2560, 2734, 2945, 3029
MfeI CAATTG 3 cut(s) 999, 1107, 1380
MflI RGATCY 3 cut(s) 16, 2373, 2480
MhlI GDGCHC 7 cut(s) 8, 130, 216, 900, 954, 1525, 1750
MlsI TGGCCA 1 cut(s) 1866
MluNI TGGCCA 1 cut(s) 1866
MlyI GAGTC 7 cut(s) 60, 218, 287, 809, 1672, 3047, 3054
Mox20I TGGCCA 1 cut(s) 1866
MroXI GAANNNNTTC 3 cut(s) 297, 1737, 2092
MscI TGGCCA 1 cut(s) 1866
MseI TTAA 4 cut(s) 564, 1538, 2123, 2760
MslI CAYNNNNRTG 4 cut(s) 1725, 1956, 1995, 2654
Msp20I TGGCCA 1 cut(s) 1866
MspA1I CMGCKG 4 cut(s) 124, 325, 2358, 2577
MspR9I CCNGG 4 cut(s) 699, 1007, 2118, 2598
MunI CAATTG 3 cut(s) 999, 1107, 1380
Mva1269I GAATGC 1 cut(s) 1395
MvaI CCWGG 4 cut(s) 699, 1007, 2118, 2598
MvnI CGCG 1 cut(s) 124
NcoI CCATGG 2 cut(s) 234, 1676
NdeI CATATG 1 cut(s) 2740
NheI GCTAGC 1 cut(s) 1511
NmuCI GTSAC 4 cut(s) 1132, 2560, 2734, 3029
NspI RCATGY 4 cut(s) 855, 1322, 1583, 1843
OliI CACNNNNGTG 1 cut(s) 1995
PaeI GCATGC 1 cut(s) 1583
PaeR7I CTCGAG 1 cut(s) 755
PagI TCATGA 2 cut(s) 2310, 2622
PasI CCCWGGG 1 cut(s) 1006
PceI AGGCCT 2 cut(s) 517, 2497
PciI ACATGT 2 cut(s) 851, 1839
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 1612
PctI GAATGC 1 cut(s) 1395
PdmI GAANNNNTTC 3 cut(s) 297, 1737, 2092
PfeI GAWTC 6 cut(s) 148, 298, 448, 820, 1101, 1487
PleI GAGTC 7 cut(s) 60, 218, 286, 808, 1671, 3046, 3054
PpsI GAGTC 7 cut(s) 60, 218, 286, 808, 1671, 3046, 3054
Ppu21I YACGTR 1 cut(s) 2213
PpuMI RGGWCCY 1 cut(s) 2889
PscI ACATGT 2 cut(s) 851, 1839
PshAI GACNNNNGTC 2 cut(s) 2423, 2741
Psp124BI GAGCTC 2 cut(s) 216, 1750
Psp5II RGGWCCY 1 cut(s) 2889
Psp6I CCWGG 4 cut(s) 697, 1005, 2116, 2596
PspFI CCCAGC 2 cut(s) 218, 2697
PspGI CCWGG 4 cut(s) 697, 1005, 2116, 2596
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 5 cut(s) 4, 5, 386, 1786, 2889
PspPPI RGGWCCY 1 cut(s) 2889
PstI CTGCAG 4 cut(s) 355, 616, 1232, 2881
PsuI RGATCY 3 cut(s) 16, 2373, 2480
PvuII CAGCTG 3 cut(s) 325, 2358, 2577
RsaI GTAC 8 cut(s) 334, 937, 1037, 1058, 1644, 1844, 2145, 2522
RsaNI GTAC 8 cut(s) 333, 936, 1036, 1057, 1643, 1843, 2144, 2521
RseI CAYNNNNRTG 4 cut(s) 1725, 1956, 1995, 2654
SacI GAGCTC 2 cut(s) 216, 1750
SacII CCGCGG 1 cut(s) 125
SaqAI TTAA 4 cut(s) 564, 1538, 2123, 2760
Sau96I GGNCC 5 cut(s) 4, 5, 386, 1786, 2889
ScaI AGTACT 1 cut(s) 2145
SchI GAGTC 7 cut(s) 60, 218, 287, 809, 1672, 3047, 3054
ScrFI CCNGG 4 cut(s) 699, 1007, 2118, 2598
SduI GDGCHC 7 cut(s) 8, 130, 216, 900, 954, 1525, 1750
SfaNI GCATC 8 cut(s) 670, 733, 1354, 1363, 1513, 1867, 2059, 2068
SfcI CTRYAG 5 cut(s) 351, 612, 1228, 2421, 2877
Sfr274I CTCGAG 1 cut(s) 755
Sfr303I CCGCGG 1 cut(s) 125
SgrBI CCGCGG 1 cut(s) 125
SinI GGWCC 3 cut(s) 386, 1786, 2889
SlaI CTCGAG 1 cut(s) 755
SmiMI CAYNNNNRTG 4 cut(s) 1725, 1956, 1995, 2654
SmlI CTYRAG 2 cut(s) 425, 755
SmoI CTYRAG 2 cut(s) 425, 755
SnaBI TACGTA 1 cut(s) 2213
SphI GCATGC 1 cut(s) 1583
SseBI AGGCCT 2 cut(s) 517, 2497
SspI AATATT 1 cut(s) 2163
SspMI CTAG 6 cut(s) 72, 632, 1347, 1512, 1622, 1716
SstI GAGCTC 2 cut(s) 216, 1750
StuI AGGCCT 2 cut(s) 517, 2497
StyD4I CCNGG 4 cut(s) 697, 1005, 2116, 2596
StyI CCWWGG 5 cut(s) 234, 1299, 1676, 1715, 2395
TaaI ACNGT 9 cut(s) 935, 1123, 1270, 1727, 2422, 2729, 2871, 2945, 3094
TaiI ACGT 4 cut(s) 1224, 1609, 2215, 3036
TatI WGTACW 4 cut(s) 332, 1056, 2143, 2520
TauI GCSGC 2 cut(s) 124, 920
TfiI GAWTC 6 cut(s) 148, 298, 448, 820, 1101, 1487
Tru1I TTAA 4 cut(s) 564, 1538, 2123, 2760
Tru9I TTAA 4 cut(s) 564, 1538, 2123, 2760
TscAI CASTG 8 cut(s) 65, 976, 1237, 1732, 2116, 2352, 2661, 2876
TseFI GTSAC 4 cut(s) 1132, 2560, 2734, 3029
Tsp45I GTSAC 4 cut(s) 1132, 2560, 2734, 3029
TspGWI ACGGA 4 cut(s) 271, 310, 2316, 2695
TspRI CASTG 8 cut(s) 65, 976, 1237, 1732, 2116, 2352, 2661, 2876
VneI GTGCAC 1 cut(s) 896
VpaK11BI GGWCC 3 cut(s) 386, 1786, 2889
XagI CCTNNNNNAGG 1 cut(s) 755
XapI RAATTY 1 cut(s) 2762
XceI RCATGY 4 cut(s) 855, 1322, 1583, 1843
XcmI CCANNNNNNNNNTGG 2 cut(s) 1204, 1796
XhoI CTCGAG 1 cut(s) 755
XmaJI CCTAGG 1 cut(s) 1715
XmiI GTMKAC 1 cut(s) 1458
XmnI GAANNNNTTC 3 cut(s) 297, 1737, 2092
XspI CTAG 6 cut(s) 72, 632, 1347, 1512, 1622, 1716
ZraI GACGTC 1 cut(s) 1222
ZrmI AGTACT 1 cut(s) 2145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.