pycom03g01500

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006441
Physical Location & Seq
Reverse (-)
1135085 .. 1136726
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g01500.1

Sequence Viewer

Length: 417 bp
ATGATTACATGGGATCAGGTTGCGATTGGCAAGGAAACTGGAAAGAGGCTTGGTATGGGAACAAACATGTACCCATCTTCCGCATTGCTTGGCCAAGACAAGGATGCAGCCATAGCTTCCCTCCCTGTAGACGAGTTGATTAAGAAGGCTGATGGATTTGCTGGAGTATTTGCTGAACACAAATATGAAATTGTAAAGAGGCTGCAGGAGAGGAAGCACATCTGTGGAATGACTGGAGATGGTGTCAATGACGCTCCTGCTTTGAAAAAAGCAGATATTGGTATTGCTGTAGTTGATGCTACTGATGCTGCAAGAGGTGCTTCAGATATTGTTCTCACTCAACCAGGGCTAAGTGTGATCATAAGTGCAGTACTGACAAGCAGAGCCATATTCCAGAGGATGAAGAACTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

139

Amino Acids

14.72

Weight (kDa)

7.83

Isoelectric Point (pI)

23.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 12 - 92 3.9e-07 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 129
AciI CCGC 1 cut(s) 81
AclWI GGATC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 306
AfaI GTAC 2 cut(s) 71, 372
AfiI CCNNNNNNNGG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 1 cut(s) 265
AjnI CCWGG 1 cut(s) 343
AleI CACNNNNGTG 1 cut(s) 222
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
AlwI GGATC 1 cut(s) 21
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 3 cut(s) 107, 202, 308
BalI TGGCCA 1 cut(s) 93
BbvI GCAGC 3 cut(s) 119, 189, 295
BccI CCATC 3 cut(s) 82, 146, 233
BciT130I CCWGG 1 cut(s) 345
BclI TGATCA 1 cut(s) 357
BfmI CTRYAG 3 cut(s) 126, 203, 288
BisI GCNGC 3 cut(s) 108, 203, 309
BlsI GCNGC 3 cut(s) 109, 204, 310
BmcAI AGTACT 1 cut(s) 372
Bme1390I CCNGG 1 cut(s) 345
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 3 cut(s) 94, 286, 295
BpmI CTGGAG 2 cut(s) 183, 255
BsaJI CCNNGG 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse1I ACTGG 2 cut(s) 43, 238
Bse3DI GCAATG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 109, 405
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 83
BseNI ACTGG 2 cut(s) 43, 238
BseXI GCAGC 3 cut(s) 119, 189, 295
BsgI GTGCAG 1 cut(s) 387
BshFI GGCC 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 100
BsnI GGCC 1 cut(s) 93
Bsp143I GATC 2 cut(s) 13, 357
BspACI CCGC 1 cut(s) 81
BspANI GGCC 1 cut(s) 93
BspMAI CTGCAG 1 cut(s) 207
BspPI GGATC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 83
BsrI ACTGG 2 cut(s) 43, 238
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 2 cut(s) 13, 357
Bst2UI CCWGG 1 cut(s) 345
BstAPI GCANNNNNTGC 1 cut(s) 317
BstDEI CTNAG 1 cut(s) 350
BstF5I GGATG 2 cut(s) 109, 405
BstKTI GATC 2 cut(s) 16, 360
BstMBI GATC 2 cut(s) 13, 357
BstMWI GCNNNNNNNGC 3 cut(s) 113, 305, 317
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 3 cut(s) 126, 203, 288
BstV1I GCAGC 3 cut(s) 119, 189, 295
BsuRI GGCC 1 cut(s) 93
BtsCI GGATG 2 cut(s) 109, 405
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 70, 371
CviAII CATG 2 cut(s) 9, 67
CviJI RGCY 8 cut(s) 49, 93, 110, 116, 149, 202, 349, 386
CviKI_1 RGCY 8 cut(s) 49, 93, 110, 116, 149, 202, 349, 386
CviQI GTAC 2 cut(s) 70, 371
DdeI CTNAG 1 cut(s) 350
DpnI GATC 2 cut(s) 15, 359
DpnII GATC 2 cut(s) 13, 357
EaeI YGGCCR 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 343
FaeI CATG 2 cut(s) 12, 70
FaiI YATR 8 cut(s) 10, 56, 68, 113, 186, 362, 389, 411
FalI AAGNNNNNCTT 2 cut(s) 304, 336
FatI CATG 2 cut(s) 8, 66
FbaI TGATCA 1 cut(s) 357
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 3 cut(s) 108, 203, 309
FokI GGATG 2 cut(s) 116, 412
Fsp4HI GCNGC 3 cut(s) 108, 203, 309
GluI GCNGC 3 cut(s) 108, 203, 309
GsuI CTGGAG 2 cut(s) 183, 255
HaeIII GGCC 1 cut(s) 93
HgaI GACGC 1 cut(s) 260
Hin1II CATG 2 cut(s) 12, 70
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 1 cut(s) 394
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 139
HpyCH4V TGCA 4 cut(s) 107, 205, 311, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 305, 317
HpyF3I CTNAG 1 cut(s) 350
Hsp92II CATG 2 cut(s) 12, 70
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 2 cut(s) 13, 357
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 3 cut(s) 119, 189, 295
LweI GCATC 3 cut(s) 94, 286, 295
MalI GATC 2 cut(s) 15, 359
MboI GATC 2 cut(s) 13, 357
MboII GAAGA 2 cut(s) 69, 415
MlsI TGGCCA 1 cut(s) 93
MluCI AATT 1 cut(s) 189
MluNI TGGCCA 1 cut(s) 93
MnlI CCTC 6 cut(s) 39, 131, 192, 204, 308, 390
Mox20I TGGCCA 1 cut(s) 93
MscI TGGCCA 1 cut(s) 93
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 2 cut(s) 183, 222
Msp20I TGGCCA 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 345
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 113, 305, 317
NdeII GATC 2 cut(s) 13, 357
NlaIII CATG 2 cut(s) 12, 70
NspI RCATGY 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 222
PciI ACATGT 1 cut(s) 66
PkrI GCNGC 3 cut(s) 109, 204, 310
PscI ACATGT 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PstI CTGCAG 1 cut(s) 207
RsaI GTAC 2 cut(s) 71, 372
RsaNI GTAC 2 cut(s) 70, 371
RseI CAYNNNNRTG 2 cut(s) 183, 222
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 3 cut(s) 108, 203, 309
Sau3AI GATC 2 cut(s) 13, 357
ScaI AGTACT 1 cut(s) 372
ScrFI CCNGG 1 cut(s) 345
SetI ASST 3 cut(s) 21, 118, 319
SfaNI GCATC 3 cut(s) 94, 286, 295
SfcI CTRYAG 3 cut(s) 126, 203, 288
SmiMI CAYNNNNRTG 2 cut(s) 183, 222
Sse9I AATT 1 cut(s) 189
SsiI CCGC 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 343
TasI AATT 1 cut(s) 189
TatI WGTACW 1 cut(s) 370
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TseI GCWGC 3 cut(s) 107, 202, 308
TspDTI ATGAA 2 cut(s) 201, 416
XceI RCATGY 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 129
ZrmI AGTACT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.