RchiOBHm_Chr6g0304901

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006441
Physical Location & Seq
Forward (+)
64043181 .. 64050000
6820 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27399

Sequence Viewer

Length: 2865 bp
ATGAGCGGCGCCAAGGCCATCAGCCTCGAGGAGATCAAAAACGAGTCCGTCGATCTGGAACGGATTCCAATTGAGGAAGTCTTTGAGCAGTTGAAATGTGCAAGGGAAGGTCTTACCTCCGAGGAGGGTCAGAACAGGCTCCAAGTCTTTGGACCAAACAAGTTAGAGGAGAAAAAGGAAAACAAGCTACTGAAGTTTTTGGGGTTCATGTGGAATCCCTTGTCATGGGTCATGGAAGCCGCTGCTGTGATGGCTATTGCCCTGGCAAATGGTGGTGGAAGGCCTCCAGATTGGCAGGATTTTGTCGGAATTATGGTCCTGCTGGTCGTTAACTCTACCATCAGTTTTATTGAGGAAAACAATGCTGGTAATGCGGCTGCTGCACTTATGGCTGGTCTTGCTCCTAAGACTAAGGTCCTCAGAGATGGTCGATGGACAGAGCAAGAGGCTTCAATTTTGGTTCCAGGAGACATAATCAGCATTAAGTTGGGAGATATAGTGCCTGCTGATGCTCGCCTTCTTGAGGGTGATCCTCTGAAGATTGACCAGTCTGCCCTAACCGGCGAGTCCCTTGCTGTCACTAAAAATCCTGGAGATGAAGTGTTTTCTGGCTCAACATGTAAACAGGGTGAAATTGAAGCAGTGGTCATTGCAACTGGTGTGCACACCTTCTTTGGTAAAGCTGCACATCTTGTGGACAGCACCAACAATGTTGGGCATTTTCAGCAAGTTCTCACAGCAATTGGAAACTTCTGCATTTGTTCAATTGGTGTCGGAATACTTATTGAAATTGTTGTCATGTACCCAATTCAGAAGCGCAAGTACAGGGATGGGATTGATAATTTGCTGGTTCTCTTGATTGGTGGAATCCCAATTGCTATGCCGACTGTTTTATCTGTCACAATGGCTATAGGTTCTCACAGGCTTTCTCAGCAAGGTGCTATTACTAAGAGAATGACTGCCATTGAGGAAATGGCTGGCATGGATGTACTGTGCAGTGATAAGACTGGGACCTTGACCCTGAACAAGCTGACAGTTGATAGAAATCTGATTGAAGTGTTCCCCAAGGGTGTGGAGAAAGAGCATGTTATCCTTCTTGCAGCAAGGGCTTCTAGAACTGAAAATCAGGATGCTATTGATGCTGCCATAGTAAATATGCTTGCTGATCCAAAGGAGGCGCGAGCTGGTGTCAGAGAAATCCATTTCCTTCCATTCAACCCTGTTGACAAGAGGACTGCTTTGACCTACATTGATTCTGATGGGAATTGGCATCGATCTAGCAAAGGTGCCCCTGAGCAGATATTAACCCTATGCAACTGCAAGGAAGATTTCAAGAAAAAGGTTCATGCCGTGATTGATAAATTTGCTGAACGTGGGCTTCGATCTCTTGGGGTTGCAAGACAGCAAATTCCTGAGAAAACAAAAGAGAGTCCAGGTGCACCATGGCAGTTTGTTGGTTTGTTGCCATTATTTGATCCTCCCAGGCATGACAGTGCAGAAACTATTCGCAGAGCTCTCAATCTTGGTGTGAATGTGAAGATGATCACTGGGGATCAGCTCGCCATTGGCAAGGAAACTGGACGGCGGCTTGGTATGGGAACAAACATGTATCCATCTTCTGCATTGCTCGGCCAAGACAAGGATGCGTCCATTTCTGCCCTCCCCATTGACGAATTGATTGAGAAGGCTGATGGATTTGCTGGAGTATTTCCAGAGCACAAATATGAAATTGTAAGGAGGCTGCAAGAGAGGAAGCACATCTGTGGAATGACTGGAGATGGTGTCAACGATGCTCCTGCTTTGAAAAAAGCAGACATTGGAATTGCTGTAGCTGATGCTACTGATGCTGCTAGAGGTGCTTCAGATATTGTTCTGACTGAACCTGGATTAAGTGTGATAATAAGCGCAGTACTTACAAGCCGGGCCATATTTCAGAGGATGAAGAACTATACTATCTACGCCGTTTCAATTACCATACGTATAGTGTTTGGCTTTATGCTCATCGCTTTGATATGGCAGTTTGACTTTGCACCCTTCATGGTATTGATCATTGCTATACTAAATGATGGAACGATCATGACAATATCCAAGGACCGAGTGAAACCATCTCCAATGCCAGACAGCTGGAAACTGAAAGAAATTTTTGCTACTGGCATTGTTCTTGGAGGTTACATGGCCTTGATGACAGTAGTGTTCTTTTGGTTGATGAAAGATACAGACTTCTTCTCGGAGACGTTTGGTGTGCGATCTTTGAGGAATAGCGATAGTGAAATGATGGCAGCGTTATACTTACAAGTGAGTATTATTAGCCAAGCCCTTATCTTTGTCACAAGGTCCCGAGGATTTTCCTTTGCGGATCGCCCTGGACTTCTCTTGCTTGGAGCTTTCATCATTGCTCAATTGGTGGCGACTCTGATAGCGGTGTATGCCAGCTGGGGTTTTGCACGGATAAAGGGATGTGGCTGGGGATGGGCTGGTGTTATCTGGCTTTTCAGTGTGTTGACATATATTCCTCTTGATTTTCTGAAGTTTGCAATCCGCTACATCCTTAGTGGAAAAGCTTGGGATAACCTTTTGGAGAACAAGACTGCGTTTACTACAAAGAAAGACTATGGAAAAGAGGAGAGAGAAGCACAGTGGGCTACCGCACAAAGGACACTACATGGTCTTCAACCACCTGAAAGCAGCAATATCTTCAGTGACAAGAGCAGTTACCGGGAACTGTCAGAGATTGCAGAACAAGCCAAGAGAAGGGCTGAGGTTGCAAGGCTTAGGGAGTTGACTACATTGAAGGGTCATGTTGAGTCTGTTGTGAAGCTGAAAGGGCTCGACATAGATAATATCCAACAGCATTATACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

954

Amino Acids

104.83

Weight (kDa)

6.46

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 21 - 84 6.8e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 134 - 232 4.4e-30 P-type ATPase actuator domain
Hydrolase PF00702 328 - 605 2.3e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 375 - 496 4.6e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 26
AccB1I GGYRCC 2 cut(s) 8, 1286
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 1 cut(s) 1180
AciI CCGC 8 cut(s) 6, 240, 374, 1585, 2354, 2420, 2539, 2646
AclWI GGATC 5 cut(s) 524, 1160, 1469, 1560, 2364
AcoI YGGCCR 1 cut(s) 1630
AcsI RAATTY 3 cut(s) 1361, 1407, 2139
AcuI CTGAAG 5 cut(s) 212, 557, 1845, 2546, 2680
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 4 cut(s) 803, 824, 990, 1911
AfiI CCNNNNNNNGG 5 cut(s) 225, 523, 1639, 2652, 2751
AflIII ACRYGT 2 cut(s) 617, 1605
AjnI CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
AleI CACNNNNGTG 1 cut(s) 1761
Alw21I GWGCWC 4 cut(s) 666, 1441, 1516, 1719
Alw26I GTCTC 2 cut(s) 462, 2225
Alw44I GTGCAC 2 cut(s) 662, 1437
AlwI GGATC 5 cut(s) 524, 1160, 1469, 1560, 2364
Ama87I CYCGRG 2 cut(s) 26, 2337
AoxI GGCC 5 cut(s) 15, 281, 1630, 1923, 2175
ApaLI GTGCAC 2 cut(s) 662, 1437
ApoI RAATTY 3 cut(s) 1361, 1407, 2139
Asp700I GAANNNNTTC 2 cut(s) 63, 1503
AspLEI GCGC 4 cut(s) 11, 819, 1180, 1907
AspS9I GGNCC 7 cut(s) 152, 316, 415, 1011, 1923, 2092, 2334
AsuC2I CCSGG 2 cut(s) 1922, 2717
AsuHPI GGTGA 2 cut(s) 539, 641
AvaI CYCGRG 2 cut(s) 26, 2337
AvaII GGWCC 6 cut(s) 152, 316, 415, 1011, 2092, 2334
BaeGI GKGCMC 3 cut(s) 666, 1291, 1441
BanI GGYRCC 2 cut(s) 8, 1286
BanII GRGCYC 2 cut(s) 1516, 2829
BarI GAAGNNNNNNTAC 2 cut(s) 806, 838
BbsI GAAGAC 1 cut(s) 2660
Bbv12I GWGCWC 4 cut(s) 666, 1441, 1516, 1719
BbvCI CCTCAGC 1 cut(s) 2757
BceAI ACGGC 3 cut(s) 1334, 1598, 1946
BcgI CGANNNNNNTGC 4 cut(s) 554, 588, 1778, 1812
BciT130I CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
BciVI GTATCC 1 cut(s) 1620
BclI TGATCA 2 cut(s) 1542, 2046
BcnI CCSGG 2 cut(s) 1922, 2717
BcoDI GTCTC 2 cut(s) 462, 2225
BfaI CTAG 3 cut(s) 1113, 1278, 1851
BfmI CTRYAG 2 cut(s) 909, 1827
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 1 cut(s) 1620
BmcAI AGTACT 1 cut(s) 1911
Bme1390I CCNGG 9 cut(s) 263, 465, 591, 1434, 1483, 1884, 1922, 2364, 2717
Bme18I GGWCC 6 cut(s) 152, 316, 415, 1011, 2092, 2334
BmeT110I CYCGRG 2 cut(s) 26, 2337
BmgT120I GGNCC 7 cut(s) 152, 316, 415, 1011, 1923, 2092, 2334
BmiI GGNNCC 6 cut(s) 10, 140, 462, 1012, 1288, 2336
BmrFI CCNGG 9 cut(s) 263, 465, 591, 1434, 1483, 1884, 1922, 2364, 2717
BmrI ACTGGG 2 cut(s) 1017, 1557
BmsI GCATC 8 cut(s) 499, 1120, 1129, 1279, 1633, 1780, 1825, 1834
BmuI ACTGGG 2 cut(s) 1017, 1557
BoxI GACNNNNGTC 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 2660
BpmI CTGGAG 4 cut(s) 270, 612, 1722, 1794
Bpu10I CCTNAGC 3 cut(s) 1293, 2757, 2771
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 2 cut(s) 1922, 2717
Bsa29I ATCGAT 1 cut(s) 1273
BsaAI YACGTR 1 cut(s) 1979
BsaBI GATNNNNATC 1 cut(s) 1044
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 9 cut(s) 12, 120, 261, 1065, 1442, 1481, 2088, 2338, 2362
BsaXI ACNNNNNCTCC 6 cut(s) 483, 513, 585, 615, 1767, 1797
Bsc4I CCNNNNNNNGG 5 cut(s) 225, 523, 1639, 2652, 2751
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 7 cut(s) 547, 661, 1012, 1552, 1582, 1777, 2155
Bse3DI GCAATG 4 cut(s) 648, 1622, 2049, 2391
Bse8I GATNNNNATC 1 cut(s) 1044
BseBI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
BseCI ATCGAT 1 cut(s) 1273
BseDI CCNNGG 9 cut(s) 12, 120, 261, 1065, 1442, 1481, 2088, 2338, 2362
BseGI GGATG 8 cut(s) 835, 991, 1135, 1648, 1944, 2462, 2474, 2544
BseJI GATNNNNATC 1 cut(s) 1044
BseLI CCNNNNNNNGG 5 cut(s) 225, 523, 1639, 2652, 2751
BseMI GCAATG 4 cut(s) 648, 1622, 2049, 2391
BseMII CTCAG 5 cut(s) 433, 944, 1284, 1404, 2748
BseNI ACTGG 7 cut(s) 547, 661, 1012, 1552, 1582, 1777, 2155
BseRI GAGGAG 4 cut(s) 44, 137, 182, 2636
BseSI GKGCMC 3 cut(s) 666, 1291, 1441
BseYI CCCAGC 2 cut(s) 2433, 2463
BsgI GTGCAG 4 cut(s) 366, 669, 1015, 1515
Bsh1236I CGCG 1 cut(s) 1180
BshFI GGCC 5 cut(s) 17, 283, 1632, 1925, 2177
BshNI GGYRCC 2 cut(s) 8, 1286
BshVI ATCGAT 1 cut(s) 1273
BsiHKAI GWGCWC 4 cut(s) 666, 1441, 1516, 1719
BsiHKCI CYCGRG 2 cut(s) 26, 2337
BsiSI CCGG 3 cut(s) 561, 1921, 2716
BslFI GGGAC 3 cut(s) 553, 1024, 2320
BslI CCNNNNNNNGG 5 cut(s) 225, 523, 1639, 2652, 2751
BsmAI GTCTC 2 cut(s) 462, 2225
BsmBI CGTCTC 1 cut(s) 2225
BsmFI GGGAC 3 cut(s) 553, 1024, 2320
BsnI GGCC 5 cut(s) 17, 283, 1632, 1925, 2177
BsoBI CYCGRG 2 cut(s) 26, 2337
Bsp1286I GDGCHC 6 cut(s) 666, 1291, 1441, 1516, 1719, 2829
Bsp19I CCATGG 1 cut(s) 1442
BspACI CCGC 8 cut(s) 6, 240, 374, 1585, 2354, 2420, 2539, 2646
BspANI GGCC 5 cut(s) 17, 283, 1632, 1925, 2177
BspCNI CTCAG 5 cut(s) 432, 943, 1285, 1405, 2749
BspDI ATCGAT 1 cut(s) 1273
BspFNI CGCG 1 cut(s) 1180
BspHI TCATGA 1 cut(s) 2076
BspLI GGNNCC 6 cut(s) 10, 140, 462, 1012, 1288, 2336
BspPI GGATC 5 cut(s) 524, 1160, 1469, 1560, 2364
BspT107I GGYRCC 2 cut(s) 8, 1286
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 4 cut(s) 648, 1622, 2049, 2391
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 7 cut(s) 547, 661, 1012, 1552, 1582, 1777, 2155
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 9 cut(s) 12, 120, 261, 1065, 1442, 1481, 2088, 2338, 2362
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 4 cut(s) 12, 1065, 1442, 2088
Bst2UI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
Bst4CI ACNGT 8 cut(s) 889, 993, 1036, 1493, 2188, 2637, 2724, 2860
BstACI GRCGYC 1 cut(s) 9
BstBAI YACGTR 1 cut(s) 1979
BstC8I GCNNGC 7 cut(s) 504, 514, 979, 1161, 1182, 1560, 2431
BstDSI CCRYGG 1 cut(s) 1442
BstENI CCTNNNNNAGG 1 cut(s) 521
BstF5I GGATG 8 cut(s) 835, 991, 1135, 1648, 1944, 2462, 2474, 2544
BstFNI CGCG 1 cut(s) 1180
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 4 cut(s) 11, 819, 1180, 1907
BstMAI GTCTC 2 cut(s) 462, 2225
BstNI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
BstNSI RCATGY 3 cut(s) 621, 1088, 1609
BstPAI GACNNNNGTC 1 cut(s) 413
BstSCI CCNGG 9 cut(s) 261, 463, 589, 1432, 1481, 1882, 1920, 2362, 2715
BstSFI CTRYAG 2 cut(s) 909, 1827
BstSLI GKGCMC 3 cut(s) 666, 1291, 1441
BstSNI TACGTA 1 cut(s) 1979
BstUI CGCG 1 cut(s) 1180
BstV2I GAAGAC 1 cut(s) 2660
BstXI CCANNNNNNTGG 3 cut(s) 149, 1072, 2124
Bsu15I ATCGAT 1 cut(s) 1273
BsuI GTATCC 1 cut(s) 1620
BsuRI GGCC 5 cut(s) 17, 283, 1632, 1925, 2177
BsuTUI ATCGAT 1 cut(s) 1273
BtgI CCRYGG 1 cut(s) 1442
BtgZI GCGATG 1 cut(s) 1987
BtsCI GGATG 8 cut(s) 835, 991, 1135, 1648, 1944, 2462, 2474, 2544
BtsI GCAGTG 2 cut(s) 648, 1003
BtsIMutI CAGTG 7 cut(s) 648, 1003, 1498, 1545, 2500, 2642, 2704
Cac8I GCNNGC 7 cut(s) 504, 514, 979, 1161, 1182, 1560, 2431
CciI TCATGA 1 cut(s) 2076
CfoI GCGC 4 cut(s) 11, 819, 1180, 1907
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 7 cut(s) 152, 316, 415, 1011, 1923, 2092, 2334
ClaI ATCGAT 1 cut(s) 1273
CseI GACGC 1 cut(s) 1635
Csp6I GTAC 4 cut(s) 802, 823, 989, 1910
CviQI GTAC 4 cut(s) 802, 823, 989, 1910
DinI GGCGCC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 1630
Ecl136II GAGCTC 1 cut(s) 1514
Eco105I TACGTA 1 cut(s) 1979
Eco130I CCWWGG 4 cut(s) 12, 1065, 1442, 2088
Eco147I AGGCCT 1 cut(s) 283
Eco24I GRGCYC 2 cut(s) 1516, 2829
Eco47I GGWCC 6 cut(s) 152, 316, 415, 1011, 2092, 2334
Eco53kI GAGCTC 1 cut(s) 1514
Eco57I CTGAAG 5 cut(s) 212, 557, 1845, 2546, 2680
Eco88I CYCGRG 2 cut(s) 26, 2337
EcoICRI GAGCTC 1 cut(s) 1514
EcoNI CCTNNNNNAGG 1 cut(s) 521
EcoO109I RGGNCCY 3 cut(s) 415, 1011, 2334
EcoRII CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
EcoT14I CCWWGG 4 cut(s) 12, 1065, 1442, 2088
EcoT38I GRGCYC 2 cut(s) 1516, 2829
EgeI GGCGCC 1 cut(s) 10
EheI GGCGCC 1 cut(s) 10
ErhI CCWWGG 4 cut(s) 12, 1065, 1442, 2088
Esp3I CGTCTC 1 cut(s) 2225
FaqI GGGAC 3 cut(s) 553, 1024, 2320
FbaI TGATCA 2 cut(s) 1542, 2046
FokI GGATG 8 cut(s) 842, 998, 1142, 1655, 1951, 2469, 2481, 2531
FriOI GRGCYC 2 cut(s) 1516, 2829
FspBI CTAG 3 cut(s) 1113, 1278, 1851
GlaI GCGC 4 cut(s) 10, 818, 1179, 1906
GsaI CCCAGC 2 cut(s) 2437, 2467
GsuI CTGGAG 4 cut(s) 270, 612, 1722, 1794
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 5 cut(s) 17, 283, 1632, 1925, 2177
HapII CCGG 3 cut(s) 561, 1921, 2716
HgaI GACGC 1 cut(s) 1635
HhaI GCGC 4 cut(s) 11, 819, 1180, 1907
Hin1I GRCGYC 1 cut(s) 9
Hin6I GCGC 4 cut(s) 9, 817, 1178, 1905
HinP1I GCGC 4 cut(s) 9, 817, 1178, 1905
HincII GTYRAC 5 cut(s) 331, 1225, 1786, 2502, 2781
HindII GTYRAC 5 cut(s) 331, 1225, 1786, 2502, 2781
HindIII AAGCTT 1 cut(s) 2559
HinfI GANTC 9 cut(s) 44, 64, 214, 566, 867, 1253, 1429, 2410, 2804
HpaI GTTAAC 1 cut(s) 331
HpaII CCGG 3 cut(s) 561, 1921, 2716
HphI GGTGA 2 cut(s) 539, 641
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 8 cut(s) 889, 993, 1036, 1493, 2188, 2637, 2724, 2860
HpyCH4IV ACGT 3 cut(s) 1372, 1978, 2234
HpySE526I ACGT 3 cut(s) 1372, 1978, 2234
Hsp92I GRCGYC 1 cut(s) 9
HspAI GCGC 4 cut(s) 9, 817, 1178, 1905
KasI GGCGCC 1 cut(s) 8
Ksp22I TGATCA 2 cut(s) 1542, 2046
KspAI GTTAAC 1 cut(s) 331
LmnI GCTCC 4 cut(s) 144, 406, 1798, 2381
LweI GCATC 8 cut(s) 499, 1120, 1129, 1279, 1633, 1780, 1825, 1834
MaeI CTAG 3 cut(s) 1113, 1278, 1851
MaeII ACGT 3 cut(s) 1372, 1978, 2234
MaeIII GTNAC 6 cut(s) 577, 898, 2168, 2326, 2699, 2711
MbiI CCGCTC 1 cut(s) 6
MboII GAAGA 8 cut(s) 550, 1337, 1549, 1608, 1954, 2215, 2660, 2686
MfeI CAATTG 5 cut(s) 69, 741, 765, 873, 2399
MhlI GDGCHC 6 cut(s) 666, 1291, 1441, 1516, 1719, 2829
Mly113I GGCGCC 1 cut(s) 9
MlyI GAGTC 5 cut(s) 53, 575, 1438, 2404, 2813
MmeI TCCRAC 2 cut(s) 286, 754
MroXI GAANNNNTTC 2 cut(s) 63, 1503
MseI TTAA 4 cut(s) 330, 483, 1304, 1889
MslI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
MspA1I CMGCKG 3 cut(s) 242, 2124, 2433
MspI CCGG 3 cut(s) 561, 1921, 2716
MspR9I CCNGG 9 cut(s) 263, 465, 591, 1434, 1483, 1884, 1922, 2364, 2717
MunI CAATTG 5 cut(s) 69, 741, 765, 873, 2399
MvaI CCWGG 7 cut(s) 263, 465, 591, 1434, 1483, 1884, 2364
MvnI CGCG 1 cut(s) 1180
NarI GGCGCC 1 cut(s) 9
NciI CCSGG 2 cut(s) 1922, 2717
NcoI CCATGG 1 cut(s) 1442
NlaIV GGNNCC 6 cut(s) 10, 140, 462, 1012, 1288, 2336
NmeAIII GCCGAG 1 cut(s) 1608
NmuCI GTSAC 4 cut(s) 577, 898, 2326, 2699
NspI RCATGY 3 cut(s) 621, 1088, 1609
OliI CACNNNNGTG 1 cut(s) 1761
PaeR7I CTCGAG 1 cut(s) 26
PagI TCATGA 1 cut(s) 2076
PceI AGGCCT 1 cut(s) 283
PciI ACATGT 2 cut(s) 617, 1605
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 1378
PdmI GAANNNNTTC 2 cut(s) 63, 1503
PfeI GAWTC 4 cut(s) 64, 214, 867, 1253
PfoI TCCNGGA 2 cut(s) 463, 589
PleI GAGTC 5 cut(s) 52, 574, 1437, 2404, 2812
PluTI GGCGCC 1 cut(s) 12
PpsI GAGTC 5 cut(s) 52, 574, 1437, 2404, 2812
Ppu21I YACGTR 1 cut(s) 1979
PpuMI RGGWCCY 3 cut(s) 415, 1011, 2334
PscI ACATGT 2 cut(s) 617, 1605
PshAI GACNNNNGTC 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 1516
Psp5II RGGWCCY 3 cut(s) 415, 1011, 2334
Psp6I CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
PspFI CCCAGC 2 cut(s) 2433, 2463
PspGI CCWGG 7 cut(s) 261, 463, 589, 1432, 1481, 1882, 2362
PspN4I GGNNCC 6 cut(s) 10, 140, 462, 1012, 1288, 2336
PspPI GGNCC 7 cut(s) 152, 316, 415, 1011, 1923, 2092, 2334
PspPPI RGGWCCY 3 cut(s) 415, 1011, 2334
PspXI VCTCGAGB 1 cut(s) 26
PvuII CAGCTG 2 cut(s) 2124, 2433
RsaI GTAC 4 cut(s) 803, 824, 990, 1911
RsaNI GTAC 4 cut(s) 802, 823, 989, 1910
RseI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
SacI GAGCTC 1 cut(s) 1516
SaqAI TTAA 4 cut(s) 330, 483, 1304, 1889
Sau96I GGNCC 7 cut(s) 152, 316, 415, 1011, 1923, 2092, 2334
ScaI AGTACT 1 cut(s) 1911
SchI GAGTC 5 cut(s) 53, 575, 1438, 2404, 2813
ScrFI CCNGG 9 cut(s) 263, 465, 591, 1434, 1483, 1884, 1922, 2364, 2717
SduI GDGCHC 6 cut(s) 666, 1291, 1441, 1516, 1719, 2829
SfaNI GCATC 8 cut(s) 499, 1120, 1129, 1279, 1633, 1780, 1825, 1834
SfcI CTRYAG 2 cut(s) 909, 1827
SfoI GGCGCC 1 cut(s) 10
Sfr274I CTCGAG 1 cut(s) 26
SinI GGWCC 6 cut(s) 152, 316, 415, 1011, 2092, 2334
SlaI CTCGAG 1 cut(s) 26
SmiMI CAYNNNNRTG 3 cut(s) 1491, 1722, 1761
SmlI CTYRAG 2 cut(s) 26, 521
SmoI CTYRAG 2 cut(s) 26, 521
SnaBI TACGTA 1 cut(s) 1979
SseBI AGGCCT 1 cut(s) 283
SsiI CCGC 8 cut(s) 6, 240, 374, 1585, 2354, 2420, 2539, 2646
SspDI GGCGCC 1 cut(s) 8
SspMI CTAG 3 cut(s) 1113, 1278, 1851
SstI GAGCTC 1 cut(s) 1516
StuI AGGCCT 1 cut(s) 283
StyD4I CCNGG 9 cut(s) 261, 463, 589, 1432, 1481, 1882, 1920, 2362, 2715
StyI CCWWGG 4 cut(s) 12, 1065, 1442, 2088
TaaI ACNGT 8 cut(s) 889, 993, 1036, 1493, 2188, 2637, 2724, 2860
TaiI ACGT 3 cut(s) 1375, 1981, 2237
TaqI TCGA 6 cut(s) 27, 51, 430, 1273, 1381, 2829
TaqII GACCGA 1 cut(s) 2109
TatI WGTACW 3 cut(s) 822, 988, 1909
TauI GCSGC 4 cut(s) 9, 242, 377, 1588
TfiI GAWTC 4 cut(s) 64, 214, 867, 1253
Tru1I TTAA 4 cut(s) 330, 483, 1304, 1889
Tru9I TTAA 4 cut(s) 330, 483, 1304, 1889
TscAI CASTG 7 cut(s) 648, 1003, 1498, 1552, 2500, 2642, 2704
TseFI GTSAC 4 cut(s) 577, 898, 2326, 2699
Tsp45I GTSAC 4 cut(s) 577, 898, 2326, 2699
TspDTI ATGAA 8 cut(s) 196, 612, 1334, 1740, 1955, 2026, 2222, 2377
TspGWI ACGGA 3 cut(s) 37, 76, 2461
TspRI CASTG 7 cut(s) 648, 1003, 1498, 1552, 2500, 2642, 2704
VneI GTGCAC 2 cut(s) 662, 1437
VpaK11BI GGWCC 6 cut(s) 152, 316, 415, 1011, 2092, 2334
XagI CCTNNNNNAGG 1 cut(s) 521
XapI RAATTY 3 cut(s) 1361, 1407, 2139
XbaI TCTAGA 1 cut(s) 1112
XceI RCATGY 3 cut(s) 621, 1088, 1609
XcmI CCANNNNNNNNNTGG 2 cut(s) 970, 1440
XhoI CTCGAG 1 cut(s) 26
XmnI GAANNNNTTC 2 cut(s) 63, 1503
XspI CTAG 3 cut(s) 1113, 1278, 1851
ZrmI AGTACT 1 cut(s) 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.