MD08G1133500.v1.1

Guanine nucleotide-binding protein subunit gamma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014669
Physical Location & Seq
Forward (+)
12671549 .. 12672770
1222 bp
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UTR
Exon/CDS
Intron
MD08G1133500.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGATAAGCAAGTAGAAGAAGCAAACCCATCATCTTCTCCGGCAAGAGGAAGAGAAGATGAAGGAGGAGGAGGTGGAGGAGGAGGAGAAGGAATGGCTCTGCCACTTTATTTCGAAGACAGAGCAAGAGCTGGCCAATTTCCCACTTTCATTGGCAAGCATAAGCTCGCAGCTTCCATTTCCCATCTTCAAAACCAAATCGACATCATCCAGAAAGAGCTGGACCAACTTGAGACAATAGGTGAATCCTCCATTGTATGCAAAGACCTCATTGCAAGCGTTGAATCAACATCTGATCCATTGCTTCCATGGACTAAAGGACCAGGTGAAGTAGGGTGGGATAGGTGGTTCCGAGGAGCCAACAACTCCAGAAACCACAGCCGCTGGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
G-gamma

Protein Analysis

131

Amino Acids

14.16

Weight (kDa)

5.16

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-gamma PF00631 63 - 119 6.7e-08 GGL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014669)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g30640 FvH4_2g30640 FvH4_2g30640
malus_domestica MD08G1133500.v1.1 MD15G1110600.v1.1
prunus_persica Prupe.1G462900_v2.0.a1 Prupe.2G330000_v2.0.a1
pyrus_communis pycom15g10100
rosa_chinensis RchiOBHm_Chr6g0312271
rosa_laevigata RLG00000010337
rosa_multiflora Rmu_ssc0000424.1_g000004
rosa_rugosa Rorug06G0394500 Rorug06G0394600
rosa_samantha Rh6BG522800 Rh6CG528500 Rh6DG514000
rosa_wichuraiana Rw6G044510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 382
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 1 cut(s) 133
AfiI CCNNNNNNNGG 1 cut(s) 47
AgsI TTSAA 2 cut(s) 191, 284
AjnI CCWGG 1 cut(s) 322
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 4 cut(s) 131, 166, 173, 220
AluI AGCT 4 cut(s) 131, 166, 173, 220
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 384
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 170
AspS9I GGNCC 2 cut(s) 223, 320
AsuHPI GGTGA 2 cut(s) 254, 338
AsuII TTCGAA 1 cut(s) 114
AvaII GGWCC 2 cut(s) 223, 320
BalI TGGCCA 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 123
BbvI GCAGC 1 cut(s) 182
BccI CCATC 2 cut(s) 37, 192
BciT130I CCWGG 1 cut(s) 324
BcoDI GTCTC 1 cut(s) 227
BisI GCNGC 2 cut(s) 171, 382
BlsI GCNGC 2 cut(s) 172, 383
Bme1390I CCNGG 1 cut(s) 324
Bme18I GGWCC 2 cut(s) 223, 320
BmgT120I GGNCC 2 cut(s) 223, 320
BmiI GGNNCC 2 cut(s) 350, 358
BmrFI CCNGG 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 1 cut(s) 352
Bpu14I TTCGAA 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 308, 352
Bsc4I CCNNNNNNNGG 1 cut(s) 47
Bse3DI GCAATG 2 cut(s) 270, 299
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 308, 352
BseGI GGATG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 47
BseMI GCAATG 2 cut(s) 270, 299
BseRI GAGGAG 6 cut(s) 81, 84, 93, 96, 99, 369
BseXI GCAGC 1 cut(s) 182
BshFI GGCC 1 cut(s) 135
BsiSI CCGG 1 cut(s) 41
BslI CCNNNNNNNGG 1 cut(s) 47
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 1 cut(s) 135
Bsp119I TTCGAA 1 cut(s) 114
Bsp143I GATC 2 cut(s) 295, 387
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 1 cut(s) 382
BspANI GGCC 1 cut(s) 135
BspLI GGNNCC 2 cut(s) 350, 358
BspPI GGATC 1 cut(s) 290
BspT104I TTCGAA 1 cut(s) 114
BsrDI GCAATG 2 cut(s) 270, 299
BssECI CCNNGG 2 cut(s) 308, 352
BssMI GATC 2 cut(s) 295, 387
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 324
Bst6I CTCTTC 1 cut(s) 46
BstBI TTCGAA 1 cut(s) 114
BstC8I GCNNGC 4 cut(s) 133, 158, 168, 277
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 207
BstKTI GATC 2 cut(s) 298, 390
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 2 cut(s) 295, 387
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 182
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 308
BtsCI GGATG 1 cut(s) 207
Cac8I GCNNGC 4 cut(s) 133, 158, 168, 277
CaiI CAGNNNCTG 1 cut(s) 384
Cfr13I GGNCC 2 cut(s) 223, 320
CsiI ACCWGGT 1 cut(s) 322
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 1 cut(s) 309
CviJI RGCY 8 cut(s) 98, 131, 135, 166, 173, 220, 359, 381
CviKI_1 RGCY 8 cut(s) 98, 131, 135, 166, 173, 220, 359, 381
DpnI GATC 2 cut(s) 297, 389
DpnII GATC 2 cut(s) 295, 387
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco130I CCWWGG 1 cut(s) 308
Eco47I GGWCC 2 cut(s) 223, 320
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 1 cut(s) 312
FaiI YATR 3 cut(s) 162, 259, 310
FatI CATG 1 cut(s) 308
Fnu4HI GCNGC 2 cut(s) 171, 382
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 171, 382
GluI GCNGC 2 cut(s) 171, 382
GsuI CTGGAG 1 cut(s) 352
HaeIII GGCC 1 cut(s) 135
HapII CCGG 1 cut(s) 41
Hin1II CATG 1 cut(s) 312
HinfI GANTC 2 cut(s) 245, 284
HpaII CCGG 1 cut(s) 41
HphI GGTGA 2 cut(s) 254, 338
Hpy188I TCNGA 2 cut(s) 295, 353
Hpy188III TCNNGA 2 cut(s) 211, 369
HpyAV CCTTC 2 cut(s) 56, 83
HpyCH4V TGCA 2 cut(s) 261, 275
Hsp92II CATG 1 cut(s) 312
Kzo9I GATC 2 cut(s) 295, 387
LmnI GCTCC 1 cut(s) 356
LpnPI CCDG 8 cut(s) 54, 117, 206, 224, 309, 336, 370, 382
Lsp1109I GCAGC 1 cut(s) 182
MabI ACCWGGT 1 cut(s) 322
MalI GATC 2 cut(s) 297, 389
MboI GATC 2 cut(s) 295, 387
MboII GAAGA 6 cut(s) 27, 29, 63, 68, 128, 179
MflI RGATCY 1 cut(s) 387
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 1 cut(s) 137
MluNI TGGCCA 1 cut(s) 135
Mox20I TGGCCA 1 cut(s) 135
MscI TGGCCA 1 cut(s) 135
Msp20I TGGCCA 1 cut(s) 135
MspA1I CMGCKG 1 cut(s) 384
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 324
MvaI CCWGG 1 cut(s) 324
NcoI CCATGG 1 cut(s) 308
NdeII GATC 2 cut(s) 295, 387
NlaIII CATG 1 cut(s) 312
NlaIV GGNNCC 2 cut(s) 350, 358
NspV TTCGAA 1 cut(s) 114
PfeI GAWTC 2 cut(s) 245, 284
PkrI GCNGC 2 cut(s) 172, 383
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 350, 358
PspPI GGNCC 2 cut(s) 223, 320
PstNI CAGNNNCTG 1 cut(s) 384
PsuI RGATCY 1 cut(s) 387
SatI GCNGC 2 cut(s) 171, 382
Sau3AI GATC 2 cut(s) 295, 387
Sau96I GGNCC 2 cut(s) 223, 320
ScrFI CCNGG 1 cut(s) 324
SetI ASST 9 cut(s) 76, 133, 168, 175, 222, 244, 270, 328, 347
SexAI ACCWGGT 1 cut(s) 322
SfuI TTCGAA 1 cut(s) 114
SinI GGWCC 2 cut(s) 223, 320
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 1 cut(s) 137
SsiI CCGC 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 308
TaqI TCGA 2 cut(s) 114, 201
TasI AATT 1 cut(s) 137
TauI GCSGC 1 cut(s) 384
TfiI GAWTC 2 cut(s) 245, 284
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 2 cut(s) 75, 139
VpaK11BI GGWCC 2 cut(s) 223, 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.