MD08G1162700.v1.1

protein At4g08330, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011319
Physical Location & Seq
Forward (+)
19112090 .. 19114013
1924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1162700.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGAGAAATCGAATCTTTTCAGAGGCAGTTACCTCAACGGAGCTTACCATCCTTCCTTCTCTTCTTCTTCTCGCGCCGATGTCAATTACAGCTGTGGTTCCTGTGGGTATGAGCTAAACCTATGTTCCAGCAACCGGAACACTACTACGATTGGCTCTAATAAGTATGGAAAATCTATAAAGCGAGGGATCATATCATTCTTTAACATCGATGAGAGCAGATTTACTCAGGTTGATGAATTTAAATGTAAACCCCATTTCTCTAAGCGTTCTTGGGGTTTGTTCAGCCGAAGGACTAAACTTCTTTGTCGCAAGTGTGGTAACCATATAGGAAATGCTTATGATGAGTACACTTCATCGTCTTGCCCGCTTGTGTTAGATGAATCAGATTCATCCGCAGGTCATGAAGTTACTAAATGCAGAAAATTTGATGTTAGAATTTGCGCTTTGCAGCCTTCAAATTCTGAAGAATTTGGCACTTCAATTTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.4

Weight (kDa)

8.72

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g08330 PF24046 23 - 153 1.1e-52 At4g08330 beta-tent
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08330 AT4G08330
fragaria_vesca FvH4_2g26960 FvH4_2g26960
malus_domestica MD08G1162700.v1.1 MD15G1348300.v1.1
prunus_persica Prupe.1G497200_v2.0.a1 Prupe.1G497200_v2.0.a1
pyrus_communis pycom08g14060 pycom15g31200
rosa_chinensis RchiOBHm_Chr6g0295821
rosa_laevigata RLG00000011765
rosa_multiflora Rmu_ssc0000086.1_g000001
rosa_roxburghii Rroxscaffold_7G00172150
rosa_rugosa Rorug06G0254100
rosa_samantha Rh6AG368100 Rh6BG375700 Rh6CG382000 Rh6DG368500
rosa_wichuraiana Rw6G032090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 389
AccII CGCG 1 cut(s) 75
AciI CCGC 2 cut(s) 368, 396
AclWI GGATC 1 cut(s) 197
AcsI RAATTY 5 cut(s) 239, 425, 438, 460, 470
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 135
AgsI TTSAA 2 cut(s) 459, 483
AluBI AGCT 3 cut(s) 44, 93, 115
AluI AGCT 3 cut(s) 44, 93, 115
AlwI GGATC 1 cut(s) 197
ApeKI GCWGC 1 cut(s) 451
ApoI RAATTY 5 cut(s) 239, 425, 438, 460, 470
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 2 cut(s) 77, 446
BbvI GCAGC 1 cut(s) 463
BccI CCATC 1 cut(s) 57
BfuAI ACCTGC 1 cut(s) 389
BisI GCNGC 1 cut(s) 452
BlsI GCNGC 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 100
Bsa29I ATCGAT 1 cut(s) 210
BsaWI WCCGGW 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 210
BseGI GGATG 2 cut(s) 49, 392
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMII CTCAG 1 cut(s) 242
BseXI GCAGC 1 cut(s) 463
Bsh1236I CGCG 1 cut(s) 75
BshVI ATCGAT 1 cut(s) 210
BsiSI CCGG 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 135
Bsp143I GATC 1 cut(s) 189
BspACI CCGC 2 cut(s) 368, 396
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 1 cut(s) 197
BssMI GATC 1 cut(s) 189
Bst6I CTCTTC 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 2 cut(s) 228, 264
BstEII GGTNACC 1 cut(s) 320
BstF5I GGATG 2 cut(s) 49, 392
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 2 cut(s) 77, 446
BstKTI GATC 1 cut(s) 192
BstMBI GATC 1 cut(s) 189
BstPI GGTNACC 1 cut(s) 320
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 463
Bsu15I ATCGAT 1 cut(s) 210
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 2 cut(s) 49, 392
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 368
CciI TCATGA 1 cut(s) 403
CfoI GCGC 2 cut(s) 77, 446
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 1 cut(s) 349
CviAII CATG 1 cut(s) 404
CviJI RGCY 6 cut(s) 44, 93, 115, 156, 288, 454
CviKI_1 RGCY 6 cut(s) 44, 93, 115, 156, 288, 454
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 2 cut(s) 228, 264
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
DraI TTTAAA 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 486
Eco91I GGTNACC 1 cut(s) 320
EcoO65I GGTNACC 1 cut(s) 320
FaeI CATG 1 cut(s) 407
FaiI YATR 9 cut(s) 111, 124, 168, 179, 194, 327, 329, 342, 405
FatI CATG 1 cut(s) 403
FauI CCCGC 1 cut(s) 375
Fnu4HI GCNGC 1 cut(s) 452
FokI GGATG 2 cut(s) 36, 379
Fsp4HI GCNGC 1 cut(s) 452
GlaI GCGC 2 cut(s) 76, 445
GluI GCNGC 1 cut(s) 452
HapII CCGG 1 cut(s) 136
HhaI GCGC 2 cut(s) 77, 446
Hin1II CATG 1 cut(s) 407
Hin6I GCGC 2 cut(s) 75, 444
HinP1I GCGC 2 cut(s) 75, 444
HinfI GANTC 3 cut(s) 13, 383, 389
HpaII CCGG 1 cut(s) 136
Hpy166II GTNNAC 2 cut(s) 251, 351
Hpy188I TCNGA 3 cut(s) 23, 388, 466
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 2 cut(s) 251, 351
HpyAV CCTTC 4 cut(s) 63, 67, 285, 465
HpyCH4V TGCA 2 cut(s) 420, 451
HpyF3I CTNAG 2 cut(s) 228, 264
Hsp92II CATG 1 cut(s) 407
HspAI GCGC 2 cut(s) 75, 444
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 5 cut(s) 115, 142, 149, 215, 384
Lsp1109I GCAGC 1 cut(s) 463
MaeIII GTNAC 3 cut(s) 29, 320, 409
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 4 cut(s) 54, 57, 60, 479
MluCI AATT 7 cut(s) 85, 239, 425, 438, 460, 470, 483
MnlI CCTC 3 cut(s) 17, 44, 179
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 204, 243
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 1 cut(s) 136
MvnI CGCG 1 cut(s) 75
NdeII GATC 1 cut(s) 189
NlaIII CATG 1 cut(s) 407
NlaIV GGNNCC 1 cut(s) 100
PagI TCATGA 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 13, 383, 389
PkrI GCNGC 1 cut(s) 453
PspEI GGTNACC 1 cut(s) 320
PspN4I GGNNCC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 93
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SaqAI TTAA 2 cut(s) 204, 243
SatI GCNGC 1 cut(s) 452
Sau3AI GATC 1 cut(s) 189
SetI ASST 7 cut(s) 36, 46, 95, 117, 123, 234, 403
SmiI ATTTAAAT 1 cut(s) 244
Sse9I AATT 7 cut(s) 85, 239, 425, 438, 460, 470, 483
SsiI CCGC 2 cut(s) 368, 396
SwaI ATTTAAAT 1 cut(s) 244
TaqI TCGA 2 cut(s) 11, 210
TasI AATT 7 cut(s) 85, 239, 425, 438, 460, 470, 483
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 3 cut(s) 13, 383, 389
Tru1I TTAA 2 cut(s) 204, 243
Tru9I TTAA 2 cut(s) 204, 243
TseI GCWGC 1 cut(s) 451
TspDTI ATGAA 5 cut(s) 252, 345, 381, 396, 420
TspGWI ACGGA 1 cut(s) 54
XapI RAATTY 5 cut(s) 239, 425, 438, 460, 470
XmnI GAANNNNTTC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.