Rw6G032090

protein At4g08330, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011319
Physical Location & Seq
Reverse (-)
56203670 .. 56205218
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032090.1

Sequence Viewer

Length: 882 bp
ATGATTCCTGGTTTGCCGTTAAACTCCACGTCAACACTTTGTTCATGGAGAGAAAGTCCCAAAGGCCAAAGGGGAGCGCATTCGGCATTCACACACTGTTCATCTACAATTCGACGATCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCAGAACACCCTTTTCCTACGTCTCTGAAACTACGTGTCGTTTTAGAAGATCCAGCTCAACTGCGACTAGCTCTTGGCGGTTCAACTCCACCCGAACCAGACGGGACACGTTCGTCGTCGTCGTCACGCACTTTATCCACCTCCACCAAACTCTTAACTATTTAATTCTCGGAAATCTCCGCCACCGTTGCAGAGCCACCATCGCAGCCGTTGATTTTCAGCTGGTTGTAAATAATGGAGAATCCGACGATTTCAGAGGCAGTTATCTAAATGGAGCTTACCAGCCTCCCCTATCTTCTTCTTCTCGCGCCGATGTCAATTACAGCTGTGGTGCTTGTGGTTATGAGCTAAACCTGAGCTCTAATAACCGGAACACATCTTTGATTGGCTCTAATAAGTACGGGAAATCTATAAAGAGAGGGATCATATCGTTCTTTTACATTGATGAGAGCAGATTTAGTCAGGTTGATGAACTTCAGTGTAAGCCCCATTTCTCTAGGCGTTCTTGGGGTCTGTTCAGCCGGAGAACTAAACTTCTTTGTCGCAAGTGCGGTAACCATATAGGAAATGCTTATGATGATTTTACTTCTCCGGCTTCGTACCCACTCGTATCAGATGGATCAGATTCTTCATCCTCAGGCCGTCAAATTTCCAAATGCAGAAAATATGATGTTAGAATTCGCTCTTTGCAGCCCTCGAATTCTGAAGAATGTGGCCCTTCGTTTTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

32.76

Weight (kDa)

9.7

Isoelectric Point (pI)

60.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g08330 PF24046 150 - 282 5.4e-52 At4g08330 beta-tent
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08330 AT4G08330
fragaria_vesca FvH4_2g26960 FvH4_2g26960
malus_domestica MD08G1162700.v1.1 MD15G1348300.v1.1
prunus_persica Prupe.1G497200_v2.0.a1 Prupe.1G497200_v2.0.a1
pyrus_communis pycom08g14060 pycom15g31200
rosa_chinensis RchiOBHm_Chr6g0295821
rosa_laevigata RLG00000011765
rosa_multiflora Rmu_ssc0000086.1_g000001
rosa_roxburghii Rroxscaffold_7G00172150
rosa_rugosa Rorug06G0254100
rosa_samantha Rh6AG368100 Rh6BG375700 Rh6CG382000 Rh6DG368500
rosa_wichuraiana Rw6G032090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AccII CGCG 1 cut(s) 456
AciI CCGC 3 cut(s) 226, 328, 699
AclWI GGATC 3 cut(s) 192, 578, 775
AcsI RAATTY 3 cut(s) 795, 825, 847
AcuI CTGAAG 2 cut(s) 608, 873
AfaI GTAC 2 cut(s) 548, 749
AflIII ACRYGT 2 cut(s) 182, 255
AgsI TTSAA 1 cut(s) 232
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 1 cut(s) 7
AluBI AGCT 7 cut(s) 204, 219, 370, 425, 474, 496, 507
AluI AGCT 7 cut(s) 204, 219, 370, 425, 474, 496, 507
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 1 cut(s) 175
AlwI GGATC 3 cut(s) 192, 578, 775
AoxI GGCC 3 cut(s) 64, 787, 862
ApeKI GCWGC 2 cut(s) 353, 838
ApoI RAATTY 3 cut(s) 795, 825, 847
AspLEI GCGC 2 cut(s) 79, 458
AspS9I GGNCC 1 cut(s) 863
AxyI CCTNAGG 1 cut(s) 784
BanII GRGCYC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 2 cut(s) 365, 850
BccI CCATC 2 cut(s) 356, 758
BceAI ACGGC 2 cut(s) 341, 774
BciT130I CCWGG 1 cut(s) 9
BcoDI GTCTC 1 cut(s) 175
BfaI CTAG 2 cut(s) 216, 645
BisI GCNGC 2 cut(s) 354, 839
BlsI GCNGC 2 cut(s) 355, 840
Bme1390I CCNGG 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 863
BmrFI CCNGG 1 cut(s) 9
Bpu10I CCTNAGC 1 cut(s) 503
BsaAI YACGTR 1 cut(s) 183
BsaWI WCCGGW 1 cut(s) 516
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bse21I CCTNAGG 1 cut(s) 784
BseBI CCWGG 1 cut(s) 9
BseGI GGATG 1 cut(s) 779
BseMII CTCAG 2 cut(s) 494, 798
BseXI GCAGC 2 cut(s) 365, 850
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 3 cut(s) 66, 789, 864
BsiHKAI GWGCWC 1 cut(s) 509
BsiSI CCGG 3 cut(s) 517, 670, 740
BslFI GGGAC 2 cut(s) 42, 266
BsmAI GTCTC 1 cut(s) 175
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 2 cut(s) 42, 266
BsmI GAATGC 2 cut(s) 79, 86
BsnI GGCC 3 cut(s) 66, 789, 864
Bsp1286I GDGCHC 1 cut(s) 509
Bsp143I GATC 4 cut(s) 116, 197, 570, 767
BspACI CCGC 3 cut(s) 226, 328, 699
BspANI GGCC 3 cut(s) 66, 789, 864
BspCNI CTCAG 2 cut(s) 495, 797
BspFNI CGCG 1 cut(s) 456
BspPI GGATC 3 cut(s) 192, 578, 775
BssMI GATC 4 cut(s) 116, 197, 570, 767
Bst2UI CCWGG 1 cut(s) 9
Bst4CI ACNGT 2 cut(s) 98, 335
BstBAI YACGTR 1 cut(s) 183
BstDEI CTNAG 2 cut(s) 503, 784
BstEII GGTNACC 1 cut(s) 701
BstF5I GGATG 1 cut(s) 779
BstFNI CGCG 1 cut(s) 456
BstHHI GCGC 2 cut(s) 79, 458
BstKTI GATC 4 cut(s) 119, 200, 573, 770
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 4 cut(s) 116, 197, 570, 767
BstMWI GCNNNNNNNGC 3 cut(s) 83, 336, 350
BstNI CCWGG 1 cut(s) 9
BstPI GGTNACC 1 cut(s) 701
BstSCI CCNGG 1 cut(s) 7
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 365, 850
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
Bsu36I CCTNAGG 1 cut(s) 784
BsuRI GGCC 3 cut(s) 66, 789, 864
BtgZI GCGATG 1 cut(s) 334
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 1 cut(s) 779
BtsIMutI CAGTG 2 cut(s) 94, 632
CfoI GCGC 2 cut(s) 79, 458
Cfr13I GGNCC 1 cut(s) 863
Csp6I GTAC 2 cut(s) 547, 748
CviAII CATG 1 cut(s) 45
CviQI GTAC 2 cut(s) 547, 748
DdeI CTNAG 2 cut(s) 503, 784
DpnI GATC 4 cut(s) 118, 199, 572, 769
DpnII GATC 4 cut(s) 116, 197, 570, 767
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EciI GGCGGA 1 cut(s) 317
Ecl136II GAGCTC 1 cut(s) 507
Eco24I GRGCYC 1 cut(s) 509
Eco53kI GAGCTC 1 cut(s) 507
Eco57I CTGAAG 2 cut(s) 608, 873
Eco81I CCTNAGG 1 cut(s) 784
Eco91I GGTNACC 1 cut(s) 701
EcoICRI GAGCTC 1 cut(s) 507
EcoO65I GGTNACC 1 cut(s) 701
EcoRI GAATTC 2 cut(s) 825, 847
EcoRII CCWGG 1 cut(s) 7
EcoT38I GRGCYC 1 cut(s) 509
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 1 cut(s) 48
FaiI YATR 8 cut(s) 46, 492, 560, 575, 708, 710, 723, 816
FaqI GGGAC 2 cut(s) 42, 266
FatI CATG 1 cut(s) 44
Fnu4HI GCNGC 2 cut(s) 354, 839
FokI GGATG 1 cut(s) 766
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 2 cut(s) 354, 839
FspBI CTAG 2 cut(s) 216, 645
GlaI GCGC 2 cut(s) 78, 457
GluI GCNGC 2 cut(s) 354, 839
HaeIII GGCC 3 cut(s) 66, 789, 864
HapII CCGG 3 cut(s) 517, 670, 740
HhaI GCGC 2 cut(s) 79, 458
Hin1II CATG 1 cut(s) 48
Hin6I GCGC 2 cut(s) 77, 456
HinP1I GCGC 2 cut(s) 77, 456
HincII GTYRAC 1 cut(s) 33
HindII GTYRAC 1 cut(s) 33
HinfI GANTC 3 cut(s) 4, 389, 773
HpaII CCGG 3 cut(s) 517, 670, 740
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 8 cut(s) 152, 175, 320, 394, 404, 763, 772, 853
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 5 cut(s) 117, 266, 269, 272, 398
HpyAV CCTTC 1 cut(s) 876
HpyCH4III ACNGT 2 cut(s) 98, 335
HpyCH4IV ACGT 4 cut(s) 29, 168, 182, 257
HpyCH4V TGCA 3 cut(s) 339, 807, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 336, 350
HpyF3I CTNAG 2 cut(s) 503, 784
HpySE526I ACGT 4 cut(s) 29, 168, 182, 257
Hsp92II CATG 1 cut(s) 48
HspAI GCGC 2 cut(s) 77, 456
Kzo9I GATC 4 cut(s) 116, 197, 570, 767
LmnI GCTCC 2 cut(s) 74, 422
Lsp1109I GCAGC 2 cut(s) 365, 850
MaeI CTAG 2 cut(s) 216, 645
MaeII ACGT 4 cut(s) 29, 168, 182, 257
MaeIII GTNAC 2 cut(s) 271, 701
MalI GATC 4 cut(s) 118, 199, 572, 769
MboI GATC 4 cut(s) 116, 197, 570, 767
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 509
MluCI AATT 6 cut(s) 108, 312, 466, 795, 825, 847
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 6 cut(s) 299, 398, 444, 560, 793, 853
MseI TTAA 3 cut(s) 20, 303, 311
MspA1I CMGCKG 2 cut(s) 370, 474
MspI CCGG 3 cut(s) 517, 670, 740
MspR9I CCNGG 1 cut(s) 9
Mva1269I GAATGC 2 cut(s) 79, 86
MvaI CCWGG 1 cut(s) 9
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 3 cut(s) 83, 336, 350
NdeII GATC 4 cut(s) 116, 197, 570, 767
NlaIII CATG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 2 cut(s) 79, 86
PfeI GAWTC 3 cut(s) 4, 389, 773
PkrI GCNGC 2 cut(s) 355, 840
Ppu21I YACGTR 1 cut(s) 183
Psp124BI GAGCTC 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 7
PspEI GGTNACC 1 cut(s) 701
PspGI CCWGG 1 cut(s) 7
PspPI GGNCC 1 cut(s) 863
PsuI RGATCY 1 cut(s) 197
PvuII CAGCTG 2 cut(s) 370, 474
RsaI GTAC 2 cut(s) 548, 749
RsaNI GTAC 2 cut(s) 547, 748
SacI GAGCTC 1 cut(s) 509
SaqAI TTAA 3 cut(s) 20, 303, 311
SatI GCNGC 2 cut(s) 354, 839
Sau3AI GATC 4 cut(s) 116, 197, 570, 767
Sau96I GGNCC 1 cut(s) 863
ScrFI CCNGG 1 cut(s) 9
SduI GDGCHC 1 cut(s) 509
Sse9I AATT 6 cut(s) 108, 312, 466, 795, 825, 847
SsiI CCGC 3 cut(s) 226, 328, 699
SspMI CTAG 2 cut(s) 216, 645
SstI GAGCTC 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 7
TaaI ACNGT 2 cut(s) 98, 335
TaiI ACGT 4 cut(s) 32, 171, 185, 260
TaqI TCGA 2 cut(s) 112, 845
TasI AATT 6 cut(s) 108, 312, 466, 795, 825, 847
TfiI GAWTC 3 cut(s) 4, 389, 773
Tru1I TTAA 3 cut(s) 20, 303, 311
Tru9I TTAA 3 cut(s) 20, 303, 311
TscAI CASTG 2 cut(s) 101, 632
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 2 cut(s) 353, 838
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 4 cut(s) 33, 90, 633, 768
TspRI CASTG 2 cut(s) 101, 632
XapI RAATTY 3 cut(s) 795, 825, 847
XspI CTAG 2 cut(s) 216, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.