MD15G1348300.v1.1

protein At4g08330, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011319
Physical Location & Seq
Forward (+)
41234871 .. 41237397
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1348300.v1.1.491

Sequence Viewer

Length: 495 bp
ATGGAGAAATCGAATATTTACAGAGGCAGTTACACTAACGGAGCTTACCATCCAACCTTCTCTTCCTCTTCTCGCGCCGATGTCAGTTACAGCTGTGGCTCTTGTGGCTATGAGCTGAACCTAAGTTCCAACAACCGGAACACTTCTACAATTGGCTCTAATAAGTATGGAAAATCTATAAAGCGGGGGATCATATCGTTCTTTAACATCGATGAGAGCAGATTTACTCAGGTTGATGAACTTAAGTGTAAACCCCATTTCTCTAAGCGTTCATGGGGTTTGTTCAGCCGGAGAACTAAACTTCTTTGTCGCAAGTGTGGTAACCATATAGGAAATGCTTATGATGAGTACACTTCATCTTCTTACCCACTTGTGTTAGATGGATCAGATTCATCATCAGGCCATGAAGTTACCAAATGCAGAAAATTTGATGTTAGAATTCGTGCCTTGCAGCCTTCGAATTCTGAAGAATGTGGCACTTCGTTTTTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.4

Weight (kDa)

9.08

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g08330 PF24046 23 - 153 8.1e-55 At4g08330 beta-tent
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08330 AT4G08330
fragaria_vesca FvH4_2g26960 FvH4_2g26960
malus_domestica MD08G1162700.v1.1 MD15G1348300.v1.1
prunus_persica Prupe.1G497200_v2.0.a1 Prupe.1G497200_v2.0.a1
pyrus_communis pycom08g14060 pycom15g31200
rosa_chinensis RchiOBHm_Chr6g0295821
rosa_laevigata RLG00000011765
rosa_multiflora Rmu_ssc0000086.1_g000001
rosa_roxburghii Rroxscaffold_7G00172150
rosa_rugosa Rorug06G0254100
rosa_samantha Rh6AG368100 Rh6BG375700 Rh6CG382000 Rh6DG368500
rosa_wichuraiana Rw6G032090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 75
AciI CCGC 1 cut(s) 184
AclWI GGATC 2 cut(s) 197, 391
AcsI RAATTY 3 cut(s) 425, 438, 460
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 135
AflII CTTAAG 1 cut(s) 242
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 3 cut(s) 44, 93, 115
AluI AGCT 3 cut(s) 44, 93, 115
AlwI GGATC 2 cut(s) 197, 391
AoxI GGCC 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 451
ApoI RAATTY 3 cut(s) 425, 438, 460
AspLEI GCGC 1 cut(s) 77
AsuII TTCGAA 1 cut(s) 458
BbvI GCAGC 1 cut(s) 463
BccI CCATC 2 cut(s) 57, 374
BfrI CTTAAG 1 cut(s) 242
BisI GCNGC 1 cut(s) 452
BlsI GCNGC 1 cut(s) 453
Bpu14I TTCGAA 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 210
BsaWI WCCGGW 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 210
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMII CTCAG 1 cut(s) 242
BseXI GCAGC 1 cut(s) 463
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 402
BshVI ATCGAT 1 cut(s) 210
BsiSI CCGG 2 cut(s) 136, 289
BslI CCNNNNNNNGG 1 cut(s) 135
BsnI GGCC 1 cut(s) 402
Bsp119I TTCGAA 1 cut(s) 458
Bsp143I GATC 2 cut(s) 189, 383
BspACI CCGC 1 cut(s) 184
BspANI GGCC 1 cut(s) 402
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 75
BspPI GGATC 2 cut(s) 197, 391
BspT104I TTCGAA 1 cut(s) 458
BspTI CTTAAG 1 cut(s) 242
BssMI GATC 2 cut(s) 189, 383
Bst6I CTCTTC 2 cut(s) 67, 73
BstAFI CTTAAG 1 cut(s) 242
BstBI TTCGAA 1 cut(s) 458
BstDEI CTNAG 3 cut(s) 122, 228, 264
BstEII GGTNACC 1 cut(s) 320
BstF5I GGATG 1 cut(s) 49
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 2 cut(s) 192, 386
BstMBI GATC 2 cut(s) 189, 383
BstMWI GCNNNNNNNGC 1 cut(s) 105
BstPI GGTNACC 1 cut(s) 320
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 463
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 1 cut(s) 402
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 1 cut(s) 49
CfoI GCGC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 1 cut(s) 349
CviAII CATG 2 cut(s) 273, 404
CviJI RGCY 9 cut(s) 44, 93, 99, 108, 115, 156, 288, 402, 454
CviKI_1 RGCY 9 cut(s) 44, 93, 99, 108, 115, 156, 288, 402, 454
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 3 cut(s) 122, 228, 264
DpnI GATC 2 cut(s) 191, 385
DpnII GATC 2 cut(s) 189, 383
Eam1104I CTCTTC 2 cut(s) 67, 73
EarI CTCTTC 2 cut(s) 67, 73
Eco57I CTGAAG 1 cut(s) 486
Eco91I GGTNACC 1 cut(s) 320
EcoO65I GGTNACC 1 cut(s) 320
EcoRI GAATTC 2 cut(s) 438, 460
FaeI CATG 2 cut(s) 276, 407
FaiI YATR 9 cut(s) 111, 168, 179, 194, 274, 327, 329, 342, 405
FatI CATG 2 cut(s) 272, 403
FauI CCCGC 1 cut(s) 177
Fnu4HI GCNGC 1 cut(s) 452
FokI GGATG 1 cut(s) 36
Fsp4HI GCNGC 1 cut(s) 452
GlaI GCGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 452
HaeIII GGCC 1 cut(s) 402
HapII CCGG 2 cut(s) 136, 289
HhaI GCGC 1 cut(s) 77
Hin1II CATG 2 cut(s) 276, 407
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HinfI GANTC 1 cut(s) 389
HpaII CCGG 2 cut(s) 136, 289
Hpy166II GTNNAC 2 cut(s) 251, 351
Hpy188I TCNGA 2 cut(s) 388, 466
Hpy8I GTNNAC 2 cut(s) 251, 351
HpyAV CCTTC 2 cut(s) 67, 465
HpyCH4V TGCA 2 cut(s) 420, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpyF3I CTNAG 3 cut(s) 122, 228, 264
Hsp92II CATG 2 cut(s) 276, 407
HspAI GCGC 1 cut(s) 75
Kzo9I GATC 2 cut(s) 189, 383
LmnI GCTCC 1 cut(s) 41
LpnPI CCDG 4 cut(s) 149, 215, 302, 384
Lsp1109I GCAGC 1 cut(s) 463
MaeIII GTNAC 4 cut(s) 29, 86, 320, 409
MalI GATC 2 cut(s) 191, 385
MboI GATC 2 cut(s) 189, 383
MboII GAAGA 4 cut(s) 54, 60, 351, 479
MfeI CAATTG 1 cut(s) 150
MluCI AATT 4 cut(s) 150, 425, 438, 460
MmeI TCCRAC 2 cut(s) 77, 153
MnlI CCTC 2 cut(s) 17, 76
MseI TTAA 2 cut(s) 204, 243
MspA1I CMGCKG 1 cut(s) 93
MspCI CTTAAG 1 cut(s) 242
MspI CCGG 2 cut(s) 136, 289
MunI CAATTG 1 cut(s) 150
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 105
NdeII GATC 2 cut(s) 189, 383
NlaIII CATG 2 cut(s) 276, 407
NspV TTCGAA 1 cut(s) 458
PfeI GAWTC 1 cut(s) 389
PkrI GCNGC 1 cut(s) 453
PspEI GGTNACC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 93
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SaqAI TTAA 2 cut(s) 204, 243
SatI GCNGC 1 cut(s) 452
Sau3AI GATC 2 cut(s) 189, 383
SetI ASST 6 cut(s) 46, 59, 95, 117, 123, 234
SfuI TTCGAA 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 4 cut(s) 150, 425, 438, 460
SsiI CCGC 1 cut(s) 184
SspI AATATT 1 cut(s) 16
TaqI TCGA 3 cut(s) 11, 210, 458
TasI AATT 4 cut(s) 150, 425, 438, 460
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 2 cut(s) 204, 243
Tru9I TTAA 2 cut(s) 204, 243
TseI GCWGC 1 cut(s) 451
TspDTI ATGAA 5 cut(s) 252, 261, 345, 381, 420
TspGWI ACGGA 1 cut(s) 54
Vha464I CTTAAG 1 cut(s) 242
XapI RAATTY 3 cut(s) 425, 438, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.