Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006062

Physical Location & Seq

Genomic Coordinates Forward (+)

20370206 .. 20377355

Gene Structure 7150 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1170000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGCATCCCTTCTGCTGCGTCTCCACGCTCTCCGATCGCTCCCCGGACCTCCACATGACCTCCTTCGGCTCCAGATCCGACTCCCACGCCCGCACCGCTAAAATCGATCCGAACGGCGTCCACAATCATAGCTACACCCACGCCCATTCCAGCGCCGACTACGGACGACTCAGTCAGCGCACCACGGCGGCTGCGGCTGCTGCCGCCGTGGCAGCCGCGGAGGTTGTGGCTCGGCCGCCCTCGGGAAGGGAGCAGCCGGTCGACGTCAAGATAAACGACATCGTTGGGAATGGCGTGTCGGGAATTCTGTATAAGTGGGTGAATTACGGCAAGGGGTGGCGGCCGAGGTGGTTCGTTCTGCAGGACGGAGTTTTGTCGTATTATAAGATTCACGGCCCCGATAGGATCGTAGTTACGGTTGAGACCGAGAAAGGATCAAAAGTCATCGGCGAGGAATCGGTGCGGCGAATTTCGAGTCATAAGAACGGCATCCCTCACCAGCTCCGCCGCAAGCCCTTCGGCGAAGTCCATCTCAAGGTTTCGAGCATCCGCGAAAGCAGATCAGATGATAAGAGGTTTTCGATCTTCACAGGCACAAAGAGGTTGCATTTGAGGGCAGAGACCCGAGAAGATCGATACGAATGGGTGGAAGCATTGCAGGCTGTGAAAGACATGTTCCCGCGGATCTCTAACAGCGAGTTGATGGCTCCAGTGGACAATGTCACCGTTTCCACAGAGAAGGTGAGGCAGCGGCTTGTGGAAGAAGGTGTTAGGGAGGCAGCCATTCAGGACACGGAGCAGATAATGAGGACTGAGTTCGCCGCGATGCAGAACCAGCTCCTGCTGCTCAAGCAGAAGCAGTGGCTTCTAATGGATACACTGCGACAGTTAGAGACAGAAAAGGTAGATTTGGAGAATACAGTGGTAGATGAGAGCCGACAATTGAAAGATTCCGGGGCTTCCTCTAGATCAAGGCAAGACAAATTCAGTGATATTACCATTTCAGAAGGGAGTGCAAGTGAATCGGATGAGGATAATGAAAGAGTTGAGGAAGAAACAGATGAAGAGGACAATGCTTTCTTTGATACTCGCGATTTCCTATCGTCAAGTTCTTTTAAAAGTAGTGGGTCTGAGTATCGGATATCATCTGTGTCTTCAGACGATGAAAGGATAAATGCCATTGAACCAGAAGATGGTCTTGATCCATCCATGAGATCTGTTGGAACTAACTTTCCTCTTGTCAAGCGCCGTAAGAAGTTGCCTGACCCTGTTGAAAAAGAGAAGAGTGTTAGTCTTTGGTCAATGATTAAGGATAACATTGGGAAGGATCTCACAAAAATATGTCTTCCTGTATACTTTAACGAGCCTCTATCTACTCTACAAAAGGGTTTTGAGGATTTGGAATACTCGTATCTCCTTGATCGAGCATATGAATGGGGTAAAAGGGGTAATAGCCTTATGAGGATACTTAATGTGGCTGCTTTTGCTGTGTCTGGATATGCATCAACTGAAGGAAGAAATTGCAAGCCATTCAACCCATTATTAGGGGAAACATACGAGGCTGACTATCCAGACAAAGGCCTCCAGTTCTTCTCAGAGAAGGTTAGTCATCACCCAATGATTATTGCTTGCCACTGCGAGGGTCAAGGATGGAAATTCTGGGGAGACAGCAATTTGAAGAGCAAATTCTGGGGTCGCTCGATTCAGCTTGACCCTGTTGGTGTCCTGACCTTGGAATTTGATGATGGGGAAGTTCTTCAATGGAGTAAAGTTACAACGTCCATTTACAACCTTATCTTGGGAAAGCTTTACTGTGATCACTATGGGACAATGCGAATACAGGGGAAGCATGAATATTCATGTAAGCTGAAATTTAAGGAGCAGTCTATCATTGACCGAAATCCTCACCAGGTACATGGTATAGTTCAAGATAAGAATGGGAAAACGGCTGCAACATTGGTTGGCAAATGGGATGAGAGCATGCACTATATTTTAGGAGACTGTTCAGGGAAGGGAAAAGGTTCCGACTCTTTATCTGACGCCCGCCTGCTCTGGAGGAGGAACAAGCCACCCAAATTTCCAACTAGATATAATTTAACACGCTTTGCCATCACGATGAATGAACTCACACCTGGACTGAAGGAGAAGTTGCCCCCCACAGATTCAAGGCTGAGACCCGATCAAAGGTATTTGGAAAATGGGGAGTATGAAATGGCAAATGCAGAGAAGTTGAGGCTAGAACAGAGACAGCGGCAGGCTCGAAAGATGCAAGAGCAGGGTTGGAAACCGCAGTGGTTCGCAAAGGATAAAGGAAGCGACACATACCGCTACGTCGGTGGATATTGGGAGGCAAGGGAACAAGGGAGATGGGATTCATGTCCTGACATTTTTGGGCAAGTCCCTTGTGAACCGCAACTTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Oxysterol_BP PH_11

Protein Analysis

808

Amino Acids

92.04

Weight (kDa)

6.61

Isoelectric Point (pI)

42.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	100 - 222	3.3e-12	PH domain
PH_11	PF15413	104 - 220	6.3e-16	Pleckstrin homology domain
Oxysterol_BP	PF01237	431 - 786	4.3e-132	Oxysterol-binding protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006062)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77730 AT2G31020 AT4G08180 AT4G08180 AT4G08180 AT4G08180
fragaria_vesca	FvH4_2g26250 FvH4_2g26250 FvH4_2g26250
malus_domestica	MD00G1220800.v1.1 MD08G1170000.v1.1 MD15G1357000.v1.1
prunus_persica	Prupe.1G504000_v2.0.a1 Prupe.1G504000_v2.0.a1
pyrus_communis	pycom08g14550 pycom15g31910
rosa_chinensis	RchiOBHm_Chr6g0294861
rosa_laevigata	RLG00000011836
rosa_multiflora	Rmu_co8074688.1_g000001 Rmu_sc0008966.1_g000015
rosa_roxburghii	Rroxscaffold_7G00172950
rosa_rugosa	Rorug06G0247200
rosa_samantha	Rh6AG360900 Rh6BG367500 Rh6CG374700 Rh6DG361400
rosa_wichuraiana	Rw6G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	384
AasI	GACNNNNNNGTC	1 cut(s)	171
AatII	GACGTC	1 cut(s)	267
AccB7I	CCANNNNNTGG	1 cut(s)	1193
AccI	GTMKAC	2 cut(s)	261, 1353
AccII	CGCG	5 cut(s)	218, 552, 682, 824, 1092
AclWI	GGATC	7 cut(s)	69, 101, 413, 442, 692, 1196, 1335
AcoI	YGGCCR	2 cut(s)	233, 341
AcsI	RAATTY	8 cut(s)	303, 468, 983, 1655, 1685, 1736, 1871, 2075
AcuI	CTGAAG	3 cut(s)	1140, 1530, 2159
AcyI	GRCGYC	3 cut(s)	117, 264, 2040
AfaI	GTAC	1 cut(s)	1914
AflIII	ACRYGT	1 cut(s)	672
AgsI	TTSAA	8 cut(s)	946, 1184, 1274, 1534, 1678, 1760, 1928, 2166
AjnI	CCWGG	2 cut(s)	1908, 2131
AloI	GAACNNNNNNTCC	2 cut(s)	1216, 1248
AluBI	AGCT	6 cut(s)	132, 502, 838, 1708, 1807, 1867
AluI	AGCT	6 cut(s)	132, 502, 838, 1708, 1807, 1867
Alw26I	GTCTC	8 cut(s)	25, 416, 614, 887, 1659, 1992, 2167, 2239
AlwI	GGATC	7 cut(s)	69, 101, 413, 442, 692, 1196, 1335
AlwNI	CAGNNNCTG	1 cut(s)	841
Ama87I	CYCGRG	2 cut(s)	241, 624
AoxI	GGCC	4 cut(s)	233, 341, 394, 1579
ApoI	RAATTY	8 cut(s)	303, 468, 983, 1655, 1685, 1736, 1871, 2075
ArsI	GACNNNNNNTTYG	2 cut(s)	460, 492
Asp700I	GAANNNNTTC	2 cut(s)	1755, 2020
AspLEI	GCGC	3 cut(s)	155, 180, 1248
AspS9I	GGNCC	2 cut(s)	46, 395
AsuC2I	CCSGG	2 cut(s)	44, 955
AsuHPI	GGTGA	6 cut(s)	331, 488, 715, 754, 1604, 1898
AvaI	CYCGRG	2 cut(s)	241, 624
AvaII	GGWCC	1 cut(s)	46
BbsI	GAAGAC	2 cut(s)	1146, 1337
BccI	CCATC	8 cut(s)	537, 697, 1187, 1213, 1644, 1739, 2117, 2361
BceAI	ACGGC	8 cut(s)	130, 191, 201, 343, 409, 502, 1233, 1962
BcgI	CGANNNNNNTGC	4 cut(s)	499, 533, 1005, 1039
BciT130I	CCWGG	2 cut(s)	1910, 2133
BciVI	GTATCC	2 cut(s)	868, 1458
BclI	TGATCA	1 cut(s)	1816
BcnI	CCSGG	2 cut(s)	44, 955
BcoDI	GTCTC	8 cut(s)	25, 416, 614, 887, 1659, 1992, 2167, 2239
BfaI	CTAG	3 cut(s)	966, 2085, 2237
BfmI	CTRYAG	1 cut(s)	359
BfoI	RGCGCY	2 cut(s)	156, 1249
BfuI	GTATCC	2 cut(s)	868, 1458
BglI	GCCNNNNNGGC	1 cut(s)	209
BglII	AGATCT	1 cut(s)	1214
Bme1390I	CCNGG	4 cut(s)	44, 955, 1910, 2133
Bme18I	GGWCC	1 cut(s)	46
BmeT110I	CYCGRG	2 cut(s)	241, 624
BmgT120I	GGNCC	2 cut(s)	46, 395
BmiI	GGNNCC	4 cut(s)	70, 397, 708, 2023
BmrFI	CCNGG	4 cut(s)	44, 955, 1910, 2133
BmsI	GCATC	6 cut(s)	13, 498, 555, 816, 1511, 2256
BpiI	GAAGAC	2 cut(s)	1146, 1337
BpmI	CTGGAG	4 cut(s)	55, 693, 1568, 2074
BpuEI	CTTGAG	2 cut(s)	518, 833
BpuMI	CCSGG	2 cut(s)	44, 955
Bsa29I	ATCGAT	2 cut(s)	105, 634
BsaBI	GATNNNNATC	1 cut(s)	1501
BsaHI	GRCGYC	3 cut(s)	117, 264, 2040
BsaI	GGTCTC	3 cut(s)	416, 614, 2167
BsaJI	CCNNGG	9 cut(s)	42, 183, 207, 216, 240, 344, 680, 954, 1731
BsaXI	ACNNNNNCTCC	4 cut(s)	44, 74, 1003, 1033
Bse118I	RCCGGY	1 cut(s)	256
Bse1I	ACTGG	2 cut(s)	710, 1585
Bse3DI	GCAATG	1 cut(s)	653
Bse8I	GATNNNNATC	1 cut(s)	1501
BseBI	CCWGG	2 cut(s)	1910, 2133
BseCI	ATCGAT	2 cut(s)	105, 634
BseDI	CCNNGG	9 cut(s)	42, 183, 207, 216, 240, 344, 680, 954, 1731
BseGI	GGATG	7 cut(s)	4, 489, 546, 1033, 1205, 1655, 1978
BseJI	GATNNNNATC	1 cut(s)	1501
BseMI	GCAATG	1 cut(s)	653
BseMII	CTCAG	5 cut(s)	184, 804, 1122, 1608, 2162
BseNI	ACTGG	2 cut(s)	710, 1585
BseRI	GAGGAG	1 cut(s)	2071
BseX3I	CGGCCG	2 cut(s)	233, 341
Bsh1236I	CGCG	5 cut(s)	218, 552, 682, 824, 1092
Bsh1285I	CGRYCG	4 cut(s)	37, 236, 261, 344
BshFI	GGCC	4 cut(s)	235, 343, 396, 1581
BshVI	ATCGAT	2 cut(s)	105, 634
BsiEI	CGRYCG	4 cut(s)	37, 236, 261, 344
BsiHKCI	CYCGRG	2 cut(s)	241, 624
BsiSI	CCGG	3 cut(s)	44, 257, 954
BslFI	GGGAC	2 cut(s)	1840, 2384
BsmAI	GTCTC	8 cut(s)	25, 416, 614, 887, 1659, 1992, 2167, 2239
BsmBI	CGTCTC	1 cut(s)	25
BsmFI	GGGAC	2 cut(s)	1840, 2384
BsnI	GGCC	4 cut(s)	235, 343, 396, 1581
Bso31I	GGTCTC	3 cut(s)	416, 614, 2167
BsoBI	CYCGRG	2 cut(s)	241, 624
Bsp68I	TCGCGA	1 cut(s)	1092
BspANI	GGCC	4 cut(s)	235, 343, 396, 1581
BspCNI	CTCAG	5 cut(s)	183, 805, 1123, 1607, 2163
BspDI	ATCGAT	2 cut(s)	105, 634
BspFNI	CGCG	5 cut(s)	218, 552, 682, 824, 1092
BspLI	GGNNCC	4 cut(s)	70, 397, 708, 2023
BspMAI	CTGCAG	1 cut(s)	363
BspPI	GGATC	7 cut(s)	69, 101, 413, 442, 692, 1196, 1335
BspQI	GCTCTTC	1 cut(s)	1673
BspTNI	GGTCTC	3 cut(s)	416, 614, 2167
BsrDI	GCAATG	1 cut(s)	653
BsrFI	RCCGGY	1 cut(s)	256
BsrI	ACTGG	2 cut(s)	710, 1585
BssAI	RCCGGY	1 cut(s)	256
BssECI	CCNNGG	9 cut(s)	42, 183, 207, 216, 240, 344, 680, 954, 1731
BssNAI	GTATAC	1 cut(s)	1354
BssNI	GRCGYC	3 cut(s)	117, 264, 2040
BssT1I	CCWWGG	1 cut(s)	1731
Bst1107I	GTATAC	1 cut(s)	1354
Bst2UI	CCWGG	2 cut(s)	1910, 2133
Bst4CI	ACNGT	6 cut(s)	418, 727, 888, 922, 1814, 2003
Bst6I	CTCTTC	3 cut(s)	1059, 1277, 1673
BstACI	GRCGYC	3 cut(s)	117, 264, 2040
BstC8I	GCNNGC	9 cut(s)	91, 512, 660, 1526, 1630, 1982, 2044, 2048, 2256
BstDEI	CTNAG	5 cut(s)	170, 813, 1131, 1594, 2171
BstDSI	CCRYGG	4 cut(s)	183, 207, 216, 680
BstENI	CCTNNNNNAGG	1 cut(s)	244
BstF5I	GGATG	7 cut(s)	4, 489, 546, 1033, 1205, 1655, 1978
BstFNI	CGCG	5 cut(s)	218, 552, 682, 824, 1092
BstH2I	RGCGCY	2 cut(s)	156, 1249
BstHHI	GCGC	3 cut(s)	155, 180, 1248
BstMAI	GTCTC	8 cut(s)	25, 416, 614, 887, 1659, 1992, 2167, 2239
BstMCI	CGRYCG	4 cut(s)	37, 236, 261, 344
BstNI	CCWGG	2 cut(s)	1910, 2133
BstNSI	RCATGY	2 cut(s)	676, 1984
BstSCI	CCNGG	4 cut(s)	42, 953, 1908, 2131
BstSFI	CTRYAG	1 cut(s)	359
BstUI	CGCG	5 cut(s)	218, 552, 682, 824, 1092
BstV2I	GAAGAC	2 cut(s)	1146, 1337
BstX2I	RGATCY	4 cut(s)	74, 684, 1214, 1327
BstXI	CCANNNNNNTGG	1 cut(s)	1916
BstYI	RGATCY	4 cut(s)	74, 684, 1214, 1327
BstZ17I	GTATAC	1 cut(s)	1354
BstZI	CGGCCG	2 cut(s)	233, 341
Bsu15I	ATCGAT	2 cut(s)	105, 634
BsuI	GTATCC	2 cut(s)	868, 1458
BsuRI	GGCC	4 cut(s)	235, 343, 396, 1581
BsuTUI	ATCGAT	2 cut(s)	105, 634
BtgI	CCRYGG	4 cut(s)	183, 207, 216, 680
BtgZI	GCGATG	1 cut(s)	839
BtsCI	GGATG	7 cut(s)	4, 489, 546, 1033, 1205, 1655, 1978
BtsI	GCAGTG	4 cut(s)	866, 878, 1633, 2297
BtsIMutI	CAGTG	7 cut(s)	717, 866, 878, 927, 994, 1633, 2297
BtuMI	TCGCGA	1 cut(s)	1092
Cac8I	GCNNGC	9 cut(s)	91, 512, 660, 1526, 1630, 1982, 2044, 2048, 2256
CaiI	CAGNNNCTG	1 cut(s)	841
CfoI	GCGC	3 cut(s)	155, 180, 1248
Cfr10I	RCCGGY	1 cut(s)	256
Cfr13I	GGNCC	2 cut(s)	46, 395
Cfr42I	CCGCGG	2 cut(s)	219, 683
ClaI	ATCGAT	2 cut(s)	105, 634
CseI	GACGC	3 cut(s)	7, 106, 2048
CsiI	ACCWGGT	1 cut(s)	1908
Csp6I	GTAC	1 cut(s)	1913
CviAII	CATG	8 cut(s)	55, 673, 1210, 1850, 1860, 1916, 1981, 2376
CviQI	GTAC	1 cut(s)	1913
DdeI	CTNAG	5 cut(s)	170, 813, 1131, 1594, 2171
DraI	TTTAAA	1 cut(s)	1117
DrdI	GACNNNNNNGTC	1 cut(s)	171
DseDI	GACNNNNNNGTC	1 cut(s)	171
EaeI	YGGCCR	2 cut(s)	233, 341
EagI	CGGCCG	2 cut(s)	233, 341
Eam1104I	CTCTTC	3 cut(s)	1059, 1277, 1673
EarI	CTCTTC	3 cut(s)	1059, 1277, 1673
EciI	GGCGGA	1 cut(s)	494
EclXI	CGGCCG	2 cut(s)	233, 341
Eco130I	CCWWGG	1 cut(s)	1731
Eco147I	AGGCCT	1 cut(s)	1581
Eco31I	GGTCTC	3 cut(s)	416, 614, 2167
Eco32I	GATATC	1 cut(s)	1143
Eco47I	GGWCC	1 cut(s)	46
Eco52I	CGGCCG	2 cut(s)	233, 341
Eco57I	CTGAAG	3 cut(s)	1140, 1530, 2159
Eco88I	CYCGRG	2 cut(s)	241, 624
EcoNI	CCTNNNNNAGG	1 cut(s)	244
EcoRI	GAATTC	1 cut(s)	303
EcoRII	CCWGG	2 cut(s)	1908, 2131
EcoRV	GATATC	1 cut(s)	1143
EcoT14I	CCWWGG	1 cut(s)	1731
EcoT22I	ATGCAT	2 cut(s)	6, 1504
ErhI	CCWWGG	1 cut(s)	1731
Esp3I	CGTCTC	1 cut(s)	25
FaeI	CATG	8 cut(s)	58, 676, 1213, 1853, 1863, 1919, 1984, 2379
FalI	AAGNNNNNCTT	2 cut(s)	1182, 1214
FaqI	GGGAC	2 cut(s)	1840, 2384
FatI	CATG	8 cut(s)	54, 672, 1209, 1849, 1859, 1915, 1980, 2375
FauI	CCCGC	3 cut(s)	98, 687, 2051
FauNDI	CATATG	1 cut(s)	1429
FbaI	TGATCA	1 cut(s)	1816
FblI	GTMKAC	2 cut(s)	261, 1353
FokI	GGATG	6 cut(s)	476, 533, 1040, 1192, 1662, 1985
FspBI	CTAG	3 cut(s)	966, 2085, 2237
GlaI	GCGC	3 cut(s)	154, 179, 1247
GsuI	CTGGAG	4 cut(s)	55, 693, 1568, 2074
HaeII	RGCGCY	2 cut(s)	156, 1249
HaeIII	GGCC	4 cut(s)	235, 343, 396, 1581
HapII	CCGG	3 cut(s)	44, 257, 954
HgaI	GACGC	3 cut(s)	7, 106, 2048
HhaI	GCGC	3 cut(s)	155, 180, 1248
Hin1I	GRCGYC	3 cut(s)	117, 264, 2040
Hin1II	CATG	8 cut(s)	58, 676, 1213, 1853, 1863, 1919, 1984, 2379
Hin6I	GCGC	3 cut(s)	153, 178, 1246
HinP1I	GCGC	3 cut(s)	153, 178, 1246
HincII	GTYRAC	1 cut(s)	262
HindII	GTYRAC	1 cut(s)	262
HindIII	AAGCTT	1 cut(s)	1805
HpaII	CCGG	3 cut(s)	44, 257, 954
HphI	GGTGA	6 cut(s)	331, 488, 715, 754, 1604, 1898
Hpy166II	GTNNAC	5 cut(s)	121, 262, 715, 1354, 2408
Hpy8I	GTNNAC	5 cut(s)	121, 262, 715, 1354, 2408
Hpy99I	CGWCG	2 cut(s)	266, 2336
HpyCH4III	ACNGT	6 cut(s)	418, 727, 888, 922, 1814, 2003
HpyCH4IV	ACGT	3 cut(s)	264, 1778, 2331
HpyF3I	CTNAG	5 cut(s)	170, 813, 1131, 1594, 2171
HpySE526I	ACGT	3 cut(s)	264, 1778, 2331
Hsp92I	GRCGYC	3 cut(s)	117, 264, 2040
Hsp92II	CATG	8 cut(s)	58, 676, 1213, 1853, 1863, 1919, 1984, 2379
HspAI	GCGC	3 cut(s)	153, 178, 1246
Ksp22I	TGATCA	1 cut(s)	1816
KspI	CCGCGG	2 cut(s)	219, 683
LguI	GCTCTTC	1 cut(s)	1673
LmnI	GCTCC	8 cut(s)	44, 74, 250, 507, 712, 796, 843, 1879
LweI	GCATC	6 cut(s)	13, 498, 555, 816, 1511, 2256
MabI	ACCWGGT	1 cut(s)	1908
MaeI	CTAG	3 cut(s)	966, 2085, 2237
MaeII	ACGT	3 cut(s)	264, 1778, 2331
MaeIII	GTNAC	3 cut(s)	412, 721, 1771
MfeI	CAATTG	1 cut(s)	941
MflI	RGATCY	4 cut(s)	74, 684, 1214, 1327
MlyI	GAGTC	4 cut(s)	74, 162, 484, 2021
MmeI	TCCRAC	5 cut(s)	102, 1201, 2049, 2105, 2261
Mph1103I	ATGCAT	2 cut(s)	6, 1504
MroXI	GAANNNNTTC	2 cut(s)	1755, 2020
MseI	TTAA	6 cut(s)	1116, 1308, 1359, 1470, 1875, 2096
MslI	CAYNNNNRTG	2 cut(s)	1432, 2114
MspA1I	CMGCKG	4 cut(s)	218, 682, 751, 2251
MspI	CCGG	3 cut(s)	44, 257, 954
MspR9I	CCNGG	4 cut(s)	44, 955, 1910, 2133
MunI	CAATTG	1 cut(s)	941
MvaI	CCWGG	2 cut(s)	1910, 2133
MvnI	CGCG	5 cut(s)	218, 552, 682, 824, 1092
NciI	CCSGG	2 cut(s)	44, 955
NdeI	CATATG	1 cut(s)	1429
NlaIII	CATG	8 cut(s)	58, 676, 1213, 1853, 1863, 1919, 1984, 2379
NlaIV	GGNNCC	4 cut(s)	70, 397, 708, 2023
NmeAIII	GCCGAG	2 cut(s)	211, 369
NmuCI	GTSAC	1 cut(s)	721
NruI	TCGCGA	1 cut(s)	1092
NsiI	ATGCAT	2 cut(s)	6, 1504
NspI	RCATGY	2 cut(s)	676, 1984
PaeI	GCATGC	1 cut(s)	1984
PceI	AGGCCT	1 cut(s)	1581
PciI	ACATGT	1 cut(s)	672
PciSI	GCTCTTC	1 cut(s)	1673
PdmI	GAANNNNTTC	2 cut(s)	1755, 2020
PfeI	GAWTC	7 cut(s)	388, 455, 950, 1022, 1702, 2162, 2372
PflFI	GACNNNGTC	2 cut(s)	171, 719
PflMI	CCANNNNNTGG	1 cut(s)	1193
Ple19I	CGATCG	1 cut(s)	37
PleI	GAGTC	4 cut(s)	74, 162, 483, 2021
PpsI	GAGTC	4 cut(s)	74, 162, 483, 2021
PscI	ACATGT	1 cut(s)	672
PsiI	TTATAA	1 cut(s)	384
Psp6I	CCWGG	2 cut(s)	1908, 2131
PspGI	CCWGG	2 cut(s)	1908, 2131
PspN4I	GGNNCC	4 cut(s)	70, 397, 708, 2023
PspPI	GGNCC	2 cut(s)	46, 395
PstI	CTGCAG	1 cut(s)	363
PstNI	CAGNNNCTG	1 cut(s)	841
PsuI	RGATCY	4 cut(s)	74, 684, 1214, 1327
PsyI	GACNNNGTC	2 cut(s)	171, 719
PvuI	CGATCG	1 cut(s)	37
RruI	TCGCGA	1 cut(s)	1092
RsaI	GTAC	1 cut(s)	1914
RsaNI	GTAC	1 cut(s)	1913
RseI	CAYNNNNRTG	2 cut(s)	1432, 2114
SacII	CCGCGG	2 cut(s)	219, 683
SalI	GTCGAC	1 cut(s)	260
SapI	GCTCTTC	1 cut(s)	1673
SaqAI	TTAA	6 cut(s)	1116, 1308, 1359, 1470, 1875, 2096
Sau96I	GGNCC	2 cut(s)	46, 395
SchI	GAGTC	4 cut(s)	74, 162, 484, 2021
ScrFI	CCNGG	4 cut(s)	44, 955, 1910, 2133
SexAI	ACCWGGT	1 cut(s)	1908
SfaNI	GCATC	6 cut(s)	13, 498, 555, 816, 1511, 2256
SfcI	CTRYAG	1 cut(s)	359
Sfr303I	CCGCGG	2 cut(s)	219, 683
SgrBI	CCGCGG	2 cut(s)	219, 683
SinI	GGWCC	1 cut(s)	46
SmiMI	CAYNNNNRTG	2 cut(s)	1432, 2114
SmlI	CTYRAG	3 cut(s)	533, 848, 2417
SmoI	CTYRAG	3 cut(s)	533, 848, 2417
SphI	GCATGC	1 cut(s)	1984
SseBI	AGGCCT	1 cut(s)	1581
SspI	AATATT	1 cut(s)	1856
SspMI	CTAG	3 cut(s)	966, 2085, 2237
StuI	AGGCCT	1 cut(s)	1581
StyD4I	CCNGG	4 cut(s)	42, 953, 1908, 2131
StyI	CCWWGG	1 cut(s)	1731
TaaI	ACNGT	6 cut(s)	418, 727, 888, 922, 1814, 2003
TaiI	ACGT	3 cut(s)	267, 1781, 2334
TaqI	TCGA	9 cut(s)	105, 261, 473, 542, 581, 634, 1423, 1700, 2260
TaqII	GACCGA	2 cut(s)	440, 1911
TfiI	GAWTC	7 cut(s)	388, 455, 950, 1022, 1702, 2162, 2372
Tru1I	TTAA	6 cut(s)	1116, 1308, 1359, 1470, 1875, 2096
Tru9I	TTAA	6 cut(s)	1116, 1308, 1359, 1470, 1875, 2096
TscAI	CASTG	7 cut(s)	717, 866, 885, 927, 994, 1640, 2297
TseFI	GTSAC	1 cut(s)	721
Tsp45I	GTSAC	1 cut(s)	721
TspGWI	ACGGA	3 cut(s)	177, 381, 809
TspRI	CASTG	7 cut(s)	717, 866, 885, 927, 994, 1640, 2297
Tth111I	GACNNNGTC	2 cut(s)	171, 719
Van91I	CCANNNNNTGG	1 cut(s)	1193
VpaK11BI	GGWCC	1 cut(s)	46
XagI	CCTNNNNNAGG	1 cut(s)	244
XapI	RAATTY	8 cut(s)	303, 468, 983, 1655, 1685, 1736, 1871, 2075
XbaI	TCTAGA	1 cut(s)	965
XceI	RCATGY	2 cut(s)	676, 1984
XmiI	GTMKAC	2 cut(s)	261, 1353
XmnI	GAANNNNTTC	2 cut(s)	1755, 2020
XspI	CTAG	3 cut(s)	966, 2085, 2237
ZraI	GACGTC	1 cut(s)	265
Zsp2I	ATGCAT	2 cut(s)	6, 1504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.