MD15G1357000.v1.1

Oxysterol-binding protein-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006062
Physical Location & Seq
Forward (+)
43041569 .. 43047386
5818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1357000.v1.1.491

Sequence Viewer

Length: 1959 bp
ATGCGGCGGATTTCGAGGCATAAGAACGGCATTCCTCACAAGCTCCGCCGCAAGCCTTTCGGCGAAGTCCATCTCAAGGTTTCGAGCATCCGCGAAAGCAGATCGGATGATAAGAGGTTTTCAATCTTCACAGGCACAAAGAGGTTGCATTTGAGGGCAGAGACTCGAGAAGATCGACACGAATGGGTGGAAGCATTGCAGGCTGTGAAAGACATGTTCCCGCGGATCTCCAACAGCGAGTTGATGGCTCCGGTCGACAATGTCGCCCTTTCTACTGATAAGGTGAGGCAGCGGCTTGTTGAAGAAGGTGTTAGGGAGGCTGCCATTCAGGACACGGAGCAGATAATGAGGACTGAGTTTGCCGCGTTGCAGAACCAGCTCTTGCTGCTCAAGCAGAAGCAGTGGCTCCTTATTGACACGCTGCGACAGTTAGAGACAGAAAAGGTAGATTTGGAGAATACAGTGGTAGATGAGAGCCGACAGTTGAAAGATTCTGGGGCTTCCTCTAGATCAAGGCAAGACAAATTCAGTGAAGGGAGTGCCAGTGAATCAGATGAGGATAATGAAAGAGTTGATGCTGCAGAAGAAGAAACAGATGAAGAGGACAATGCTTTCTTTGATACTCGTGATTTTCTATCATCTAGTTCTTTTAAAAGTAATGGGTCCGAGTATCGGATATCATCTGTGTCTTCAGACGATGAAGGGATAAATGCCATTGAAGCAGAAGATGGTCTTCATCCATCTATGAGATCTGTTGGAACTAACTTTCCTCTTGTCAAGCGCCGTAATAAGTTGCCGGACCCTGTTGAAAAAGAGAAGAGTGTTAGTCTTTGGTCAATGATTAAGGATAACATTGGGAAGGATCTCACAAAAATATGTCTTCCTGTATACTTTAATGAGCCTCTATCTACTCTACAAAAGGGTTTTGAGGATTTGGAATACTCGTATCTCCTTGATCGAGCATATGAATGGGGTAAAAGGGGTAATAGCCTTATGAGGATACTTAATGTGGCTGCTTTTGCTGTGTCTGGATATGCCTCAACTGAAGGAAGAATTTGCAAGCCATTCAACCCATTATTAGGGGAAACATACGAGGCTGACTATCCAGACAAAGGCCTCCGATTCTTCTCAGAGAAGGTTAGTCATCACCCCATGATTATCGCTTGCCACTGTGAGGGTCAAGGATGGAAATTCTGGGGAGACAGCAATTTGAAGAGCAAATTCTGGGGTCGCTCGATTCAGCTTGATCCTGTTGGTGTCCTGACCTTGGAATTTGATGATGGGGAAGTTCTTCAGTGGAGTAAAGTTACAACGTCCATTTACAACCTTATCTTGGGAAAGCTTTACTGTGATCACTATGGGACGATGCGATTACAAGGAAAGCATGAATATTCATGTAAGCTGAAATTTAAGGAGCAGTCTATCATTGACCGAAATCCGCACCAGGTACATGGTATAGTTCAAGATAAGAATGGGAAAACGGCTGCAACAATGTTTGGCAAATGGGATGAGAGCATGCACTACGTTTTAGGAGACTGTTCAGGGAAGGGAAAAGGATCCGACTCTTTATCTGACTCCCGCCTGCTCTGGAGGAGGAACAAGCCACCCAAGTTTCCAACTAGATACAACTTAACACGCTTTGCCATCACATTGAATGAACTCACACCTGGACTGAAGGAGAAGTTGCCCCCAACAGATTCAAGGCTCAGACCCGATCAAAGGTATTTGGAAAATGGGGAGTATGAAATGGCAAATGCAGAGAAGTTGAGGCTGGAACAGAGACAACGGCAGGCACGAAAGATGCAAGAGCAGGGTTGGAAACCGCAGTGGTTTGCAAAGGATAAAGGAAGCGACACATACGGCTACACCGGTGGATATTGGGAGGCAAGGGAACAAGGAAACTGGGATTCATGTCCTGACATTTTTGGGCAAGTCCCTTGTGAACCGCAACTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oxysterol_BP PH_11

Protein Analysis

653

Amino Acids

75.0

Weight (kDa)

6.24

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 276 - 631 6e-133 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 255, 888
AccII CGCG 3 cut(s) 93, 223, 365
AclWI GGATC 5 cut(s) 233, 870, 1241, 1551, 1564
AcsI RAATTY 6 cut(s) 524, 1053, 1190, 1220, 1271, 1406
AcuI CTGAAG 4 cut(s) 675, 1065, 1277, 1694
AfaI GTAC 1 cut(s) 1449
AfiI CCNNNNNNNGG 8 cut(s) 76, 672, 1079, 1112, 1174, 1267, 1333, 1719
AflIII ACRYGT 1 cut(s) 213
AgeI ACCGGT 1 cut(s) 1868
AjnI CCWGG 2 cut(s) 1443, 1666
AloI GAACNNNNNNTCC 2 cut(s) 751, 783
AluBI AGCT 5 cut(s) 43, 379, 1243, 1342, 1402
AluI AGCT 5 cut(s) 43, 379, 1243, 1342, 1402
Alw26I GTCTC 5 cut(s) 155, 428, 1194, 1527, 1774
AlwI GGATC 5 cut(s) 233, 870, 1241, 1551, 1564
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 1114
ApeKI GCWGC 7 cut(s) 289, 320, 385, 421, 578, 1013, 1484
ApoI RAATTY 6 cut(s) 524, 1053, 1190, 1220, 1271, 1406
AsiGI ACCGGT 1 cut(s) 1868
Asp700I GAANNNNTTC 1 cut(s) 1290
AspLEI GCGC 1 cut(s) 783
AspS9I GGNCC 2 cut(s) 663, 799
AsuHPI GGTGA 2 cut(s) 295, 1139
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 2 cut(s) 663, 799
BamHI GGATCC 1 cut(s) 1556
BauI CACGAG 1 cut(s) 624
BbsI GAAGAC 3 cut(s) 681, 725, 872
BbvI GCAGC 7 cut(s) 301, 307, 372, 408, 565, 1000, 1471
BccI CCATC 7 cut(s) 78, 238, 722, 748, 1179, 1274, 1652
BceAI ACGGC 5 cut(s) 43, 768, 1497, 1802, 1876
BcgI CGANNNNNNTGC 2 cut(s) 40, 74
BciT130I CCWGG 2 cut(s) 1445, 1668
BciVI GTATCC 1 cut(s) 993
BclI TGATCA 1 cut(s) 1351
BcoDI GTCTC 5 cut(s) 155, 428, 1194, 1527, 1774
BfaI CTAG 3 cut(s) 507, 642, 1620
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 784
BfuI GTATCC 1 cut(s) 993
BglII AGATCT 1 cut(s) 749
Bme1390I CCNGG 2 cut(s) 1445, 1668
Bme18I GGWCC 2 cut(s) 663, 799
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 2 cut(s) 663, 799
BmiI GGNNCC 5 cut(s) 249, 407, 664, 801, 1558
BmrFI CCNGG 2 cut(s) 1445, 1668
BmrI ACTGGG 1 cut(s) 1912
BmsI GCATC 4 cut(s) 96, 565, 1356, 1791
BmuI ACTGGG 1 cut(s) 1912
BpiI GAAGAC 3 cut(s) 681, 725, 872
BpmI CTGGAG 1 cut(s) 1609
BpuEI CTTGAG 2 cut(s) 59, 374
BsaJI CCNNGG 2 cut(s) 221, 1266
BsaWI WCCGGW 2 cut(s) 250, 1868
BsaXI ACNNNNNCTCC 2 cut(s) 529, 559
Bsc4I CCNNNNNNNGG 8 cut(s) 76, 672, 1079, 1112, 1174, 1267, 1333, 1719
Bse118I RCCGGY 1 cut(s) 1868
Bse1I ACTGG 2 cut(s) 543, 1907
Bse3DI GCAATG 1 cut(s) 194
BseBI CCWGG 2 cut(s) 1445, 1668
BseDI CCNNGG 2 cut(s) 221, 1266
BseGI GGATG 5 cut(s) 87, 112, 736, 1190, 1513
BseLI CCNNNNNNNGG 8 cut(s) 76, 672, 1079, 1112, 1174, 1267, 1333, 1719
BseMI GCAATG 1 cut(s) 194
BseMII CTCAG 3 cut(s) 345, 1143, 1720
BseNI ACTGG 2 cut(s) 543, 1907
BseRI GAGGAG 1 cut(s) 1606
BseXI GCAGC 7 cut(s) 301, 307, 372, 408, 565, 1000, 1471
Bsh1236I CGCG 3 cut(s) 93, 223, 365
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 1116
BshTI ACCGGT 1 cut(s) 1868
BsiEI CGRYCG 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 3 cut(s) 251, 797, 1869
BslFI GGGAC 2 cut(s) 1375, 1919
BslI CCNNNNNNNGG 8 cut(s) 76, 672, 1079, 1112, 1174, 1267, 1333, 1719
BsmAI GTCTC 5 cut(s) 155, 428, 1194, 1527, 1774
BsmFI GGGAC 2 cut(s) 1375, 1919
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 1 cut(s) 1116
BsoBI CYCGRG 1 cut(s) 165
BspANI GGCC 1 cut(s) 1116
BspCNI CTCAG 3 cut(s) 346, 1142, 1719
BspFNI CGCG 3 cut(s) 93, 223, 365
BspLI GGNNCC 5 cut(s) 249, 407, 664, 801, 1558
BspMAI CTGCAG 1 cut(s) 583
BspPI GGATC 5 cut(s) 233, 870, 1241, 1551, 1564
BspQI GCTCTTC 1 cut(s) 1208
BsrDI GCAATG 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 1868
BsrI ACTGG 2 cut(s) 543, 1907
BssAI RCCGGY 1 cut(s) 1868
BssECI CCNNGG 2 cut(s) 221, 1266
BssNAI GTATAC 1 cut(s) 889
BssSI CACGAG 1 cut(s) 624
BssT1I CCWWGG 1 cut(s) 1266
Bst1107I GTATAC 1 cut(s) 889
Bst2BI CACGAG 1 cut(s) 624
Bst2UI CCWGG 2 cut(s) 1445, 1668
Bst4CI ACNGT 6 cut(s) 429, 463, 483, 1172, 1349, 1538
Bst6I CTCTTC 3 cut(s) 594, 812, 1208
BstC8I GCNNGC 7 cut(s) 53, 201, 1061, 1165, 1517, 1583, 1791
BstDEI CTNAG 3 cut(s) 354, 1129, 1706
BstDSI CCRYGG 1 cut(s) 221
BstF5I GGATG 5 cut(s) 87, 112, 736, 1190, 1513
BstFNI CGCG 3 cut(s) 93, 223, 365
BstH2I RGCGCY 1 cut(s) 784
BstHHI GCGC 1 cut(s) 783
BstMAI GTCTC 5 cut(s) 155, 428, 1194, 1527, 1774
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 6 cut(s) 200, 376, 385, 391, 719, 1019
BstNI CCWGG 2 cut(s) 1445, 1668
BstNSI RCATGY 2 cut(s) 217, 1519
BstSCI CCNGG 2 cut(s) 1443, 1666
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 3 cut(s) 93, 223, 365
BstV1I GCAGC 7 cut(s) 301, 307, 372, 408, 565, 1000, 1471
BstV2I GAAGAC 3 cut(s) 681, 725, 872
BstX2I RGATCY 4 cut(s) 225, 749, 862, 1556
BstXI CCANNNNNNTGG 1 cut(s) 1451
BstYI RGATCY 4 cut(s) 225, 749, 862, 1556
BstZ17I GTATAC 1 cut(s) 889
BsuI GTATCC 1 cut(s) 993
BsuRI GGCC 1 cut(s) 1116
BtgI CCRYGG 1 cut(s) 221
BtsCI GGATG 5 cut(s) 87, 112, 736, 1190, 1513
BtsI GCAGTG 2 cut(s) 407, 1832
BtsIMutI CAGTG 7 cut(s) 407, 468, 535, 550, 1168, 1301, 1832
Cac8I GCNNGC 7 cut(s) 53, 201, 1061, 1165, 1517, 1583, 1791
CfoI GCGC 1 cut(s) 783
Cfr10I RCCGGY 1 cut(s) 1868
Cfr13I GGNCC 2 cut(s) 663, 799
Cfr42I CCGCGG 1 cut(s) 224
CsiI ACCWGGT 1 cut(s) 1443
Csp6I GTAC 1 cut(s) 1448
CspAI ACCGGT 1 cut(s) 1868
CviAII CATG 7 cut(s) 214, 1153, 1385, 1395, 1451, 1516, 1911
CviQI GTAC 1 cut(s) 1448
DdeI CTNAG 3 cut(s) 354, 1129, 1706
DraI TTTAAA 1 cut(s) 652
Eam1104I CTCTTC 3 cut(s) 594, 812, 1208
EarI CTCTTC 3 cut(s) 594, 812, 1208
EciI GGCGGA 2 cut(s) 22, 35
Eco130I CCWWGG 1 cut(s) 1266
Eco147I AGGCCT 1 cut(s) 1116
Eco32I GATATC 1 cut(s) 678
Eco47I GGWCC 2 cut(s) 663, 799
Eco57I CTGAAG 4 cut(s) 675, 1065, 1277, 1694
Eco88I CYCGRG 1 cut(s) 165
EcoRII CCWGG 2 cut(s) 1443, 1666
EcoRV GATATC 1 cut(s) 678
EcoT14I CCWWGG 1 cut(s) 1266
ErhI CCWWGG 1 cut(s) 1266
FaeI CATG 7 cut(s) 217, 1156, 1388, 1398, 1454, 1519, 1914
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 2 cut(s) 1375, 1919
FatI CATG 7 cut(s) 213, 1152, 1384, 1394, 1450, 1515, 1910
FauI CCCGC 2 cut(s) 228, 1586
FauNDI CATATG 1 cut(s) 964
FbaI TGATCA 1 cut(s) 1351
FblI GTMKAC 2 cut(s) 255, 888
FokI GGATG 5 cut(s) 74, 119, 723, 1197, 1520
FspBI CTAG 3 cut(s) 507, 642, 1620
GlaI GCGC 1 cut(s) 782
GsuI CTGGAG 1 cut(s) 1609
HaeII RGCGCY 1 cut(s) 784
HaeIII GGCC 1 cut(s) 1116
HapII CCGG 3 cut(s) 251, 797, 1869
HhaI GCGC 1 cut(s) 783
Hin1II CATG 7 cut(s) 217, 1156, 1388, 1398, 1454, 1519, 1914
Hin6I GCGC 1 cut(s) 781
HinP1I GCGC 1 cut(s) 781
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 1340
HinfI GANTC 9 cut(s) 163, 491, 548, 1122, 1237, 1562, 1574, 1697, 1907
HpaII CCGG 3 cut(s) 251, 797, 1869
HphI GGTGA 2 cut(s) 295, 1139
Hpy166II GTNNAC 3 cut(s) 256, 889, 1943
Hpy8I GTNNAC 3 cut(s) 256, 889, 1943
HpyAV CCTTC 8 cut(s) 299, 527, 695, 853, 1040, 1129, 1540, 1669
HpyCH4III ACNGT 6 cut(s) 429, 463, 483, 1172, 1349, 1538
HpyCH4IV ACGT 2 cut(s) 1313, 1524
HpyF10VI GCNNNNNNNGC 6 cut(s) 200, 376, 385, 391, 719, 1019
HpyF3I CTNAG 3 cut(s) 354, 1129, 1706
HpySE526I ACGT 2 cut(s) 1313, 1524
Hsp92II CATG 7 cut(s) 217, 1156, 1388, 1398, 1454, 1519, 1914
HspAI GCGC 1 cut(s) 781
Ksp22I TGATCA 1 cut(s) 1351
KspI CCGCGG 1 cut(s) 224
LguI GCTCTTC 1 cut(s) 1208
LmnI GCTCC 5 cut(s) 48, 253, 337, 411, 1414
Lsp1109I GCAGC 7 cut(s) 301, 307, 372, 408, 565, 1000, 1471
LweI GCATC 4 cut(s) 96, 565, 1356, 1791
MabI ACCWGGT 1 cut(s) 1443
MaeI CTAG 3 cut(s) 507, 642, 1620
MaeII ACGT 2 cut(s) 1313, 1524
MaeIII GTNAC 1 cut(s) 1306
MflI RGATCY 4 cut(s) 225, 749, 862, 1556
MluCI AATT 7 cut(s) 524, 1053, 1190, 1207, 1220, 1271, 1406
MlyI GAGTC 3 cut(s) 157, 1556, 1568
MmeI TCCRAC 5 cut(s) 255, 736, 1584, 1640, 1796
MroXI GAANNNNTTC 1 cut(s) 1290
MseI TTAA 6 cut(s) 651, 843, 894, 1005, 1410, 1631
MslI CAYNNNNRTG 1 cut(s) 967
MspA1I CMGCKG 2 cut(s) 223, 292
MspI CCGG 3 cut(s) 251, 797, 1869
MspR9I CCNGG 2 cut(s) 1445, 1668
Mva1269I GAATGC 1 cut(s) 30
MvaI CCWGG 2 cut(s) 1445, 1668
MvnI CGCG 3 cut(s) 93, 223, 365
MwoI GCNNNNNNNGC 6 cut(s) 200, 376, 385, 391, 719, 1019
NdeI CATATG 1 cut(s) 964
NlaIII CATG 7 cut(s) 217, 1156, 1388, 1398, 1454, 1519, 1914
NlaIV GGNNCC 5 cut(s) 249, 407, 664, 801, 1558
NspI RCATGY 2 cut(s) 217, 1519
PaeI GCATGC 1 cut(s) 1519
PaeR7I CTCGAG 1 cut(s) 165
PceI AGGCCT 1 cut(s) 1116
PciI ACATGT 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 1208
PcsI WCGNNNNNNNCGW 2 cut(s) 172, 1792
PctI GAATGC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 1290
PfeI GAWTC 6 cut(s) 491, 548, 1122, 1237, 1697, 1907
PflFI GACNNNGTC 1 cut(s) 260
PinAI ACCGGT 1 cut(s) 1868
PleI GAGTC 3 cut(s) 157, 1556, 1568
PpsI GAGTC 3 cut(s) 157, 1556, 1568
PscI ACATGT 1 cut(s) 213
Psp6I CCWGG 2 cut(s) 1443, 1666
PspGI CCWGG 2 cut(s) 1443, 1666
PspN4I GGNNCC 5 cut(s) 249, 407, 664, 801, 1558
PspPI GGNCC 2 cut(s) 663, 799
PstI CTGCAG 1 cut(s) 583
PsuI RGATCY 4 cut(s) 225, 749, 862, 1556
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 1 cut(s) 1449
RsaNI GTAC 1 cut(s) 1448
RseI CAYNNNNRTG 1 cut(s) 967
SacII CCGCGG 1 cut(s) 224
SalI GTCGAC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 1208
SaqAI TTAA 6 cut(s) 651, 843, 894, 1005, 1410, 1631
Sau96I GGNCC 2 cut(s) 663, 799
SchI GAGTC 3 cut(s) 157, 1556, 1568
ScrFI CCNGG 2 cut(s) 1445, 1668
SexAI ACCWGGT 1 cut(s) 1443
SfaNI GCATC 4 cut(s) 96, 565, 1356, 1791
SfcI CTRYAG 1 cut(s) 579
Sfr274I CTCGAG 1 cut(s) 165
Sfr303I CCGCGG 1 cut(s) 224
SgrAI CRCCGGYG 1 cut(s) 1868
SgrBI CCGCGG 1 cut(s) 224
SinI GGWCC 2 cut(s) 663, 799
SlaI CTCGAG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 967
SmlI CTYRAG 3 cut(s) 74, 165, 389
SmoI CTYRAG 3 cut(s) 74, 165, 389
SphI GCATGC 1 cut(s) 1519
Sse9I AATT 7 cut(s) 524, 1053, 1190, 1207, 1220, 1271, 1406
SseBI AGGCCT 1 cut(s) 1116
SspI AATATT 1 cut(s) 1391
SspMI CTAG 3 cut(s) 507, 642, 1620
StuI AGGCCT 1 cut(s) 1116
StyD4I CCNGG 2 cut(s) 1443, 1666
StyI CCWWGG 1 cut(s) 1266
TaaI ACNGT 6 cut(s) 429, 463, 483, 1172, 1349, 1538
TaiI ACGT 2 cut(s) 1316, 1527
TaqI TCGA 7 cut(s) 14, 83, 166, 175, 255, 958, 1235
TaqII GACCGA 1 cut(s) 1446
TasI AATT 7 cut(s) 524, 1053, 1190, 1207, 1220, 1271, 1406
TauI GCSGC 4 cut(s) 7, 51, 295, 365
TfiI GAWTC 6 cut(s) 491, 548, 1122, 1237, 1697, 1907
Tru1I TTAA 6 cut(s) 651, 843, 894, 1005, 1410, 1631
Tru9I TTAA 6 cut(s) 651, 843, 894, 1005, 1410, 1631
TscAI CASTG 7 cut(s) 407, 468, 535, 550, 1175, 1301, 1832
TseI GCWGC 7 cut(s) 289, 320, 385, 421, 578, 1013, 1484
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 7 cut(s) 407, 468, 535, 550, 1175, 1301, 1832
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 2 cut(s) 663, 799
XapI RAATTY 6 cut(s) 524, 1053, 1190, 1220, 1271, 1406
XbaI TCTAGA 1 cut(s) 506
XceI RCATGY 2 cut(s) 217, 1519
XhoI CTCGAG 1 cut(s) 165
XmiI GTMKAC 2 cut(s) 255, 888
XmnI GAANNNNTTC 1 cut(s) 1290
XspI CTAG 3 cut(s) 507, 642, 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.