Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008452

Physical Location & Seq

Genomic Coordinates Forward (+)

20849868 .. 20855325

Gene Structure 5458 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1173100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGAAAGAAGTGAACCCACCTTAGTTCCAGAATGGTTGAGAGGTACAGGAAGTGCTACTGGGGGTGGCAGTTCAGCCCACCATTTTGCATCATCTTCTTCTCACTCAGAGGTTTCTTCTTTGGCTAATCATCTGAGAAATAGAACCTCAAAGAGCATAAGTGATTTTGATACACCGCGCTCTGCTTCCCTGGACCGTTCTTCCTCTTCCAATTCCCGGAGGAGTTCTAGTAATGGTTCTGCAAAGCATGCTTACAGTAGTTTTAACAGAAGCCACCGTGATAAGGACCGTGAGAAGGAAAAAGACAGATTAAATTTTGGAGACCATTGGGACCGTGACTCTTCTGACCCTCTGGGAAACATTTTCACCAGTAGGGTTGAGAAGGATACTTTGCGACGTTCTCAGTCTATGGTGTCCAGGAAGCAGAGTGAGCCTTCGCCCCGAAGAGCTGATATTGACTCAAAAAGTTCTAGTAACAGCAATCATCAAAATGGCAATGGTTTGCTTTCTGGGGTTGGAGTTGGTAGTCAGAAGGTAGTGTTTGATAAGGATTTCCCCTCACTTGGAACTGAAGAAAGGCCAGCTGCAACTGATATTGGAAGAGTTCCATCCCCTGGATTTAGCACAGTTGTTCAAAGCTTGCCTGTTGGTAGTTCAGCTTTAATTGGTGGGGAGGGGTGGACCTCAGCTCTGGCAGAGGTCCCTTCGACTATCATTGCGAGCAGTAACTCAGGGTCCTTTCCTGTTCAACCAACTGTTGCTGCTACTTCAAGTTCTGGGGCATCAACTGCAATGTCTGGTCTTAACATGGCTGAAGCATTAGCACAAGCTCCAGCAAAAGCTCGCACTGCTCCTCAGTTGTCTATCAAGACACAGAGGCTTGAGGAATTGGCAATCAAGCAATCAAGGCAATTAATTCCAGTGACACCTTCAATGCCCAAAGCTTCTGTTCTCAATTCTTCTGACAAATCAAAGCCCAAAACAGCAGCCAGAACTGGGGAAACAAATGCGCCAGTTAAAGTTGGACAACAGCAGCCCTCTCAATTGCACAATCAATCTCTTCGTGGTGGTTCTGTCAAGTCTGATGCTCCAAAGACTTCTAAGTTCCTTGTTCTCACCAAAGGATGGGAAAATGGAGTCTCCTCTTCACCAAAAGATGTCACTAGCCCAACAAGTAATGCCAGCAGCAGAGCAGCAAATAGCCCACTTGCTGTTGCTCCCCCAGTTGCATCCACTCCCTTAAGGAGCCCCAACAACCAGAAGCTTTTATCTGTGGAGCGCAAGGTAGCTGCCTTGGATCTGAAATCTGGATCTACGTTGGAAAAGAGACCTTCTTTGTCTCAAGTCCAGAGCCGGAATGATTTTTTCAAACGCTTGAAGAACAAAACCTTGATGAACAGTTCAATTACTCTCCCAGATCCAGGCCCAAACATTTCATCTCCCACTATGGAGAAATCTGGTGAAATACACAGGGAAGTATTCAGTGATCCTGCGAGCCCTCATACCATTGAAAATGGTGCTCTGGTGACTGGCAATGGTGATAGCTCTGAAGATGTTCAGAAGTTTTCCGATACTGCCCTCAGTGCGGCTGTATATCCGGATGAGGAAGAGGCTCGCTTTCTTCGATCTCTTGGCTGGGAGGAAAACTCTGGGGATGATGGTGGCCTTACAGAAGAAGAGATAAATGCATTTTATGACCAGTACATGAAATCACGACCGTCTCTGAAATGCCGAGGCATGCAGCCAAAGCTTGCTGTGGCGTCTGAATCTCCTGCGACTAATTTGGGTGGGGCTCGGTCTGAATTGAGCTCAACTGACTCTGGGTCTGAAGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

612

Amino Acids

64.84

Weight (kDa)

9.2

Isoelectric Point (pI)

65.35

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008452)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36990 AT4G08510 AT4G08510
fragaria_vesca	FvH4_5g38960 FvH4_5g38960 FvH4_5g38960 FvH4_5g38960
malus_domestica	MD08G1173100.v1.1 MD15G1358400.v1.1
prunus_persica	Prupe.1G505900_v2.0.a1
pyrus_communis	pycom15g32070 pycom15g32080
rosa_chinensis	RchiOBHm_Chr7g0240931
rosa_laevigata	RLG00000000613
rosa_multiflora	Rmu_sc0001324.1_g000003 Rmu_sc0031641.1_g000001
rosa_roxburghii	Rroxscaffold_3G00219410
rosa_rugosa	Rorug07G0340300
rosa_samantha	Rh7AG494800 Rh7BG466400 Rh7CG512900 Rh7DG479700
rosa_wichuraiana	Rw7G041850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	614, 1125
AccII	CGCG	1 cut(s)	178
AccIII	TCCGGA	1 cut(s)	1597
AciI	CCGC	2 cut(s)	176, 1586
AclWI	GGATC	4 cut(s)	1305, 1318, 1412, 1481
AcsI	RAATTY	1 cut(s)	313
AcuI	CTGAAG	3 cut(s)	591, 834, 1569
AcyI	GRCGYC	1 cut(s)	1760
AfaI	GTAC	2 cut(s)	46, 1703
AfiI	CCNNNNNNNGG	9 cut(s)	216, 283, 295, 563, 614, 996, 1125, 1421, 1585
AflII	CTTAAG	1 cut(s)	1240
AgsI	TTSAA	8 cut(s)	635, 749, 771, 933, 1369, 1378, 1404, 1511
AhdI	GACNNNNNGTC	1 cut(s)	1822
AjnI	CCWGG	4 cut(s)	189, 416, 613, 1420
Alw21I	GWGCWC	2 cut(s)	1522, 1811
Alw26I	GTCTC	5 cut(s)	315, 1144, 1321, 1344, 1725
AlwI	GGATC	4 cut(s)	1305, 1318, 1412, 1481
Aor13HI	TCCGGA	1 cut(s)	1597
AoxI	GGCC	3 cut(s)	578, 1423, 1663
ApeKI	GCWGC	8 cut(s)	584, 761, 986, 1033, 1185, 1193, 1289, 1741
ApoI	RAATTY	1 cut(s)	313
AseI	ATTAAT	1 cut(s)	914
Asp700I	GAANNNNTTC	1 cut(s)	1554
AspLEI	GCGC	3 cut(s)	180, 1012, 1281
AspS9I	GGNCC	7 cut(s)	193, 286, 331, 681, 700, 735, 1424
AsuC2I	CCSGG	1 cut(s)	217
AsuHPI	GGTGA	6 cut(s)	358, 1108, 1140, 1472, 1537, 1550
AvaII	GGWCC	6 cut(s)	193, 286, 331, 681, 700, 735
BanII	GRGCYC	4 cut(s)	1250, 1499, 1795, 1811
Bbv12I	GWGCWC	2 cut(s)	1522, 1811
BbvCI	CCTCAGC	1 cut(s)	685
BbvI	GCAGC	8 cut(s)	571, 748, 998, 1045, 1197, 1205, 1276, 1753
BccI	CCATC	3 cut(s)	616, 1119, 1652
BciT130I	CCWGG	4 cut(s)	191, 418, 615, 1422
BciVI	GTATCC	1 cut(s)	379
BcnI	CCSGG	1 cut(s)	217
BcoDI	GTCTC	5 cut(s)	315, 1144, 1321, 1344, 1725
BfaI	CTAG	3 cut(s)	228, 471, 1164
BfrI	CTTAAG	1 cut(s)	1240
BfuI	GTATCC	1 cut(s)	379
BisI	GCNGC	9 cut(s)	585, 762, 987, 1034, 1186, 1194, 1290, 1587, 1742
BlsI	GCNGC	9 cut(s)	586, 763, 988, 1035, 1187, 1195, 1291, 1588, 1743
Bme1390I	CCNGG	5 cut(s)	191, 217, 418, 615, 1422
Bme18I	GGWCC	6 cut(s)	193, 286, 331, 681, 700, 735
BmeRI	GACNNNNNGTC	1 cut(s)	1822
BmgT120I	GGNCC	7 cut(s)	193, 286, 331, 681, 700, 735, 1424
BmiI	GGNNCC	4 cut(s)	332, 702, 736, 1247
BmrFI	CCNGG	5 cut(s)	191, 217, 418, 615, 1422
BmrI	ACTGGG	3 cut(s)	69, 1005, 1217
BmsI	GCATC	4 cut(s)	98, 791, 1075, 1238
BmuI	ACTGGG	3 cut(s)	69, 1005, 1217
BplI	GAGNNNNNCTC	2 cut(s)	1631, 1663
BpmI	CTGGAG	1 cut(s)	816
Bpu10I	CCTNAGC	1 cut(s)	685
BpuEI	CTTGAG	2 cut(s)	902, 1326
BpuMI	CCSGG	1 cut(s)	217
BsaHI	GRCGYC	1 cut(s)	1760
BsaI	GGTCTC	2 cut(s)	315, 1321
BsaJI	CCNNGG	4 cut(s)	189, 613, 1293, 1732
BsaWI	WCCGGW	1 cut(s)	1597
BsaXI	ACNNNNNCTCC	2 cut(s)	214, 244
Bsc4I	CCNNNNNNNGG	9 cut(s)	216, 283, 295, 563, 614, 996, 1125, 1421, 1585
Bse1I	ACTGG	8 cut(s)	64, 369, 920, 1000, 1013, 1223, 1534, 1699
Bse3DI	GCAATG	4 cut(s)	502, 714, 798, 1540
BseAI	TCCGGA	1 cut(s)	1597
BseBI	CCWGG	4 cut(s)	191, 418, 615, 1422
BseDI	CCNNGG	4 cut(s)	189, 613, 1293, 1732
BseGI	GGATG	5 cut(s)	608, 1130, 1229, 1606, 1660
BseLI	CCNNNNNNNGG	9 cut(s)	216, 283, 295, 563, 614, 996, 1125, 1421, 1585
BseMI	GCAATG	4 cut(s)	502, 714, 798, 1540
BseMII	CTCAG	7 cut(s)	120, 125, 416, 699, 744, 869, 1594
BseNI	ACTGG	8 cut(s)	64, 369, 920, 1000, 1013, 1223, 1534, 1699
BseRI	GAGGAG	3 cut(s)	235, 843, 1132
BseXI	GCAGC	8 cut(s)	571, 748, 998, 1045, 1197, 1205, 1276, 1753
BseYI	CCCAGC	1 cut(s)	1635
Bsh1236I	CGCG	1 cut(s)	178
Bsh1285I	CGRYCG	1 cut(s)	1718
BshFI	GGCC	3 cut(s)	580, 1425, 1665
BsiEI	CGRYCG	1 cut(s)	1718
BsiHKAI	GWGCWC	2 cut(s)	1522, 1811
BsiSI	CCGG	3 cut(s)	217, 1354, 1598
BslFI	GGGAC	2 cut(s)	344, 686
BslI	CCNNNNNNNGG	9 cut(s)	216, 283, 295, 563, 614, 996, 1125, 1421, 1585
BsmAI	GTCTC	5 cut(s)	315, 1144, 1321, 1344, 1725
BsmBI	CGTCTC	1 cut(s)	1725
BsmFI	GGGAC	2 cut(s)	344, 686
BsnI	GGCC	3 cut(s)	580, 1425, 1665
Bso31I	GGTCTC	2 cut(s)	315, 1321
Bsp1286I	GDGCHC	5 cut(s)	1250, 1499, 1522, 1795, 1811
Bsp13I	TCCGGA	1 cut(s)	1597
Bsp143I	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
BspACI	CCGC	2 cut(s)	176, 1586
BspANI	GGCC	3 cut(s)	580, 1425, 1665
BspCNI	CTCAG	7 cut(s)	119, 126, 415, 698, 743, 868, 1593
BspEI	TCCGGA	1 cut(s)	1597
BspFNI	CGCG	1 cut(s)	178
BspLI	GGNNCC	4 cut(s)	332, 702, 736, 1247
BspPI	GGATC	4 cut(s)	1305, 1318, 1412, 1481
BspQI	GCTCTTC	1 cut(s)	439
BspTI	CTTAAG	1 cut(s)	1240
BspTNI	GGTCTC	2 cut(s)	315, 1321
BsrDI	GCAATG	4 cut(s)	502, 714, 798, 1540
BsrI	ACTGG	8 cut(s)	64, 369, 920, 1000, 1013, 1223, 1534, 1699
BssECI	CCNNGG	4 cut(s)	189, 613, 1293, 1732
BssMI	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
BssNI	GRCGYC	1 cut(s)	1760
BssT1I	CCWWGG	1 cut(s)	1293
Bst2UI	CCWGG	4 cut(s)	191, 418, 615, 1422
Bst4CI	ACNGT	9 cut(s)	197, 257, 278, 290, 335, 628, 757, 1400, 1719
Bst6I	CTCTTC	8 cut(s)	211, 346, 439, 595, 1065, 1150, 1602, 1671
BstACI	GRCGYC	1 cut(s)	1760
BstAFI	CTTAAG	1 cut(s)	1240
BstAPI	GCANNNNNTGC	2 cut(s)	248, 788
BstDEI	CTNAG	9 cut(s)	22, 106, 134, 402, 685, 730, 855, 1101, 1580
BstF5I	GGATG	5 cut(s)	608, 1130, 1229, 1606, 1660
BstFNI	CGCG	1 cut(s)	178
BstHHI	GCGC	3 cut(s)	180, 1012, 1281
BstKTI	GATC	5 cut(s)	1300, 1313, 1420, 1489, 1628
BstMAI	GTCTC	5 cut(s)	315, 1144, 1321, 1344, 1725
BstMBI	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
BstMCI	CGRYCG	1 cut(s)	1718
BstMWI	GCNNNNNNNGC	7 cut(s)	248, 430, 788, 848, 907, 1583, 1747
BstNI	CCWGG	4 cut(s)	191, 418, 615, 1422
BstNSI	RCATGY	2 cut(s)	251, 1741
BstSCI	CCNGG	5 cut(s)	189, 215, 416, 613, 1420
BstUI	CGCG	1 cut(s)	178
BstV1I	GCAGC	8 cut(s)	571, 748, 998, 1045, 1197, 1205, 1276, 1753
BstX2I	RGATCY	3 cut(s)	1297, 1310, 1417
BstYI	RGATCY	3 cut(s)	1297, 1310, 1417
BsuI	GTATCC	1 cut(s)	379
BsuRI	GGCC	3 cut(s)	580, 1425, 1665
BtsCI	GGATG	5 cut(s)	608, 1130, 1229, 1606, 1660
BtsI	GCAGTG	1 cut(s)	846
BtsIMutI	CAGTG	4 cut(s)	846, 927, 1489, 1588
CfoI	GCGC	3 cut(s)	180, 1012, 1281
Cfr13I	GGNCC	7 cut(s)	193, 286, 331, 681, 700, 735, 1424
CseI	GACGC	1 cut(s)	1749
Csp6I	GTAC	2 cut(s)	45, 1702
CviAII	CATG	4 cut(s)	248, 808, 1705, 1738
CviQI	GTAC	2 cut(s)	45, 1702
DdeI	CTNAG	9 cut(s)	22, 106, 134, 402, 685, 730, 855, 1101, 1580
DpnI	GATC	5 cut(s)	1299, 1312, 1419, 1488, 1627
DpnII	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
DriI	GACNNNNNGTC	1 cut(s)	1822
Eam1104I	CTCTTC	8 cut(s)	211, 346, 439, 595, 1065, 1150, 1602, 1671
Eam1105I	GACNNNNNGTC	1 cut(s)	1822
EarI	CTCTTC	8 cut(s)	211, 346, 439, 595, 1065, 1150, 1602, 1671
Ecl136II	GAGCTC	1 cut(s)	1809
Eco130I	CCWWGG	1 cut(s)	1293
Eco24I	GRGCYC	4 cut(s)	1250, 1499, 1795, 1811
Eco31I	GGTCTC	2 cut(s)	315, 1321
Eco47I	GGWCC	6 cut(s)	193, 286, 331, 681, 700, 735
Eco53kI	GAGCTC	1 cut(s)	1809
Eco57I	CTGAAG	3 cut(s)	591, 834, 1569
EcoICRI	GAGCTC	1 cut(s)	1809
EcoO109I	RGGNCCY	2 cut(s)	700, 735
EcoRII	CCWGG	4 cut(s)	189, 416, 613, 1420
EcoT14I	CCWWGG	1 cut(s)	1293
EcoT22I	ATGCAT	1 cut(s)	1690
EcoT38I	GRGCYC	4 cut(s)	1250, 1499, 1795, 1811
ErhI	CCWWGG	1 cut(s)	1293
Esp3I	CGTCTC	1 cut(s)	1725
FaeI	CATG	4 cut(s)	251, 811, 1708, 1741
FaqI	GGGAC	2 cut(s)	344, 686
FatI	CATG	4 cut(s)	247, 807, 1704, 1737
Fnu4HI	GCNGC	9 cut(s)	585, 762, 987, 1034, 1186, 1194, 1290, 1587, 1742
FokI	GGATG	5 cut(s)	595, 1137, 1216, 1613, 1667
FriOI	GRGCYC	4 cut(s)	1250, 1499, 1795, 1811
Fsp4HI	GCNGC	9 cut(s)	585, 762, 987, 1034, 1186, 1194, 1290, 1587, 1742
FspBI	CTAG	3 cut(s)	228, 471, 1164
GlaI	GCGC	3 cut(s)	179, 1011, 1280
GluI	GCNGC	9 cut(s)	585, 762, 987, 1034, 1186, 1194, 1290, 1587, 1742
GsaI	CCCAGC	1 cut(s)	1639
GsuI	CTGGAG	1 cut(s)	816
HaeIII	GGCC	3 cut(s)	580, 1425, 1665
HapII	CCGG	3 cut(s)	217, 1354, 1598
HgaI	GACGC	1 cut(s)	1749
HhaI	GCGC	3 cut(s)	180, 1012, 1281
Hin1I	GRCGYC	1 cut(s)	1760
Hin1II	CATG	4 cut(s)	251, 811, 1708, 1741
Hin6I	GCGC	3 cut(s)	178, 1010, 1279
HinP1I	GCGC	3 cut(s)	178, 1010, 1279
HindIII	AAGCTT	4 cut(s)	637, 942, 1262, 1748
HinfI	GANTC	5 cut(s)	338, 458, 1137, 1766, 1817
HpaII	CCGG	3 cut(s)	217, 1354, 1598
HphI	GGTGA	6 cut(s)	358, 1108, 1140, 1472, 1537, 1550
Hpy166II	GTNNAC	2 cut(s)	14, 681
Hpy188III	TCNNGA	6 cut(s)	29, 868, 1308, 1348, 1598, 1713
Hpy8I	GTNNAC	2 cut(s)	14, 681
Hpy99I	CGWCG	1 cut(s)	399
HpyAV	CCTTC	7 cut(s)	289, 376, 444, 526, 714, 939, 1341
HpyCH4III	ACNGT	9 cut(s)	197, 257, 278, 290, 335, 628, 757, 1400, 1719
HpyCH4IV	ACGT	2 cut(s)	397, 1316
HpyCH4V	TGCA	8 cut(s)	89, 242, 587, 791, 1048, 1229, 1688, 1741
HpyF10VI	GCNNNNNNNGC	7 cut(s)	248, 430, 788, 848, 907, 1583, 1747
HpyF3I	CTNAG	9 cut(s)	22, 106, 134, 402, 685, 730, 855, 1101, 1580
HpySE526I	ACGT	2 cut(s)	397, 1316
Hsp92I	GRCGYC	1 cut(s)	1760
Hsp92II	CATG	4 cut(s)	251, 811, 1708, 1741
HspAI	GCGC	3 cut(s)	178, 1010, 1279
Kpn2I	TCCGGA	1 cut(s)	1597
Kzo9I	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
LguI	GCTCTTC	1 cut(s)	439
LmnI	GCTCC	6 cut(s)	835, 856, 1093, 1222, 1245, 1276
Lsp1109I	GCAGC	8 cut(s)	571, 748, 998, 1045, 1197, 1205, 1276, 1753
LweI	GCATC	4 cut(s)	98, 791, 1075, 1238
MaeI	CTAG	3 cut(s)	228, 471, 1164
MaeII	ACGT	2 cut(s)	397, 1316
MaeIII	GTNAC	6 cut(s)	335, 473, 725, 922, 1159, 1525
MalI	GATC	5 cut(s)	1299, 1312, 1419, 1488, 1627
MboI	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
MfeI	CAATTG	1 cut(s)	1043
MflI	RGATCY	3 cut(s)	1297, 1310, 1417
MhlI	GDGCHC	5 cut(s)	1250, 1499, 1522, 1795, 1811
MlyI	GAGTC	4 cut(s)	332, 452, 1146, 1811
MmeI	TCCRAC	3 cut(s)	496, 1003, 1299
Mph1103I	ATGCAT	1 cut(s)	1690
MroI	TCCGGA	1 cut(s)	1597
MroXI	GAANNNNTTC	1 cut(s)	1554
MseI	TTAA	7 cut(s)	264, 311, 662, 804, 914, 1017, 1241
MslI	CAYNNNNRTG	1 cut(s)	489
MspA1I	CMGCKG	1 cut(s)	584
MspCI	CTTAAG	1 cut(s)	1240
MspI	CCGG	3 cut(s)	217, 1354, 1598
MspR9I	CCNGG	5 cut(s)	191, 217, 418, 615, 1422
MunI	CAATTG	1 cut(s)	1043
MvaI	CCWGG	4 cut(s)	191, 418, 615, 1422
MvnI	CGCG	1 cut(s)	178
MwoI	GCNNNNNNNGC	7 cut(s)	248, 430, 788, 848, 907, 1583, 1747
NciI	CCSGG	1 cut(s)	217
NdeII	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
NlaIII	CATG	4 cut(s)	251, 811, 1708, 1741
NlaIV	GGNNCC	4 cut(s)	332, 702, 736, 1247
NmeAIII	GCCGAG	1 cut(s)	1757
NmuCI	GTSAC	4 cut(s)	335, 922, 1159, 1525
NsiI	ATGCAT	1 cut(s)	1690
NspI	RCATGY	2 cut(s)	251, 1741
PaeI	GCATGC	2 cut(s)	251, 1741
PciSI	GCTCTTC	1 cut(s)	439
PcsI	WCGNNNNNNNCGW	1 cut(s)	1621
PdmI	GAANNNNTTC	1 cut(s)	1554
PfeI	GAWTC	1 cut(s)	1766
PflMI	CCANNNNNTGG	2 cut(s)	614, 1125
PfoI	TCCNGGA	2 cut(s)	215, 416
PkrI	GCNGC	9 cut(s)	586, 763, 988, 1035, 1187, 1195, 1291, 1588, 1743
PleI	GAGTC	4 cut(s)	332, 452, 1145, 1811
PpsI	GAGTC	4 cut(s)	332, 452, 1145, 1811
PpuMI	RGGWCCY	2 cut(s)	700, 735
PshBI	ATTAAT	1 cut(s)	914
Psp124BI	GAGCTC	1 cut(s)	1811
Psp5II	RGGWCCY	2 cut(s)	700, 735
Psp6I	CCWGG	4 cut(s)	189, 416, 613, 1420
PspFI	CCCAGC	1 cut(s)	1635
PspGI	CCWGG	4 cut(s)	189, 416, 613, 1420
PspN4I	GGNNCC	4 cut(s)	332, 702, 736, 1247
PspPI	GGNCC	7 cut(s)	193, 286, 331, 681, 700, 735, 1424
PspPPI	RGGWCCY	2 cut(s)	700, 735
PsuI	RGATCY	3 cut(s)	1297, 1310, 1417
PvuII	CAGCTG	1 cut(s)	584
RsaI	GTAC	2 cut(s)	46, 1703
RsaNI	GTAC	2 cut(s)	45, 1702
RseI	CAYNNNNRTG	1 cut(s)	489
SacI	GAGCTC	1 cut(s)	1811
SapI	GCTCTTC	1 cut(s)	439
SaqAI	TTAA	7 cut(s)	264, 311, 662, 804, 914, 1017, 1241
SatI	GCNGC	9 cut(s)	585, 762, 987, 1034, 1186, 1194, 1290, 1587, 1742
Sau3AI	GATC	5 cut(s)	1297, 1310, 1417, 1486, 1625
Sau96I	GGNCC	7 cut(s)	193, 286, 331, 681, 700, 735, 1424
SchI	GAGTC	4 cut(s)	332, 452, 1146, 1811
ScrFI	CCNGG	5 cut(s)	191, 217, 418, 615, 1422
SduI	GDGCHC	5 cut(s)	1250, 1499, 1522, 1795, 1811
SfaNI	GCATC	4 cut(s)	98, 791, 1075, 1238
SinI	GGWCC	6 cut(s)	193, 286, 331, 681, 700, 735
SmiMI	CAYNNNNRTG	1 cut(s)	489
SmlI	CTYRAG	3 cut(s)	881, 1240, 1341
SmoI	CTYRAG	3 cut(s)	881, 1240, 1341
SphI	GCATGC	2 cut(s)	251, 1741
SsiI	CCGC	2 cut(s)	176, 1586
SspMI	CTAG	3 cut(s)	228, 471, 1164
SstI	GAGCTC	1 cut(s)	1811
StyD4I	CCNGG	5 cut(s)	189, 215, 416, 613, 1420
StyI	CCWWGG	1 cut(s)	1293
TaaI	ACNGT	9 cut(s)	197, 257, 278, 290, 335, 628, 757, 1400, 1719
TaiI	ACGT	2 cut(s)	400, 1319
TaqI	TCGA	2 cut(s)	707, 1624
TaqII	GACCGA	1 cut(s)	1785
TatI	WGTACW	1 cut(s)	1701
TauI	GCSGC	1 cut(s)	1589
TfiI	GAWTC	1 cut(s)	1766
Tru1I	TTAA	7 cut(s)	264, 311, 662, 804, 914, 1017, 1241
Tru9I	TTAA	7 cut(s)	264, 311, 662, 804, 914, 1017, 1241
TscAI	CASTG	4 cut(s)	853, 927, 1489, 1588
TseFI	GTSAC	4 cut(s)	335, 922, 1159, 1525
TseI	GCWGC	8 cut(s)	584, 761, 986, 1033, 1185, 1193, 1289, 1741
Tsp45I	GTSAC	4 cut(s)	335, 922, 1159, 1525
TspDTI	ATGAA	3 cut(s)	1409, 1425, 1721
TspRI	CASTG	4 cut(s)	853, 927, 1489, 1588
Van91I	CCANNNNNTGG	2 cut(s)	614, 1125
Vha464I	CTTAAG	1 cut(s)	1240
VpaK11BI	GGWCC	6 cut(s)	193, 286, 331, 681, 700, 735
VspI	ATTAAT	1 cut(s)	914
XapI	RAATTY	1 cut(s)	313
XceI	RCATGY	2 cut(s)	251, 1741
XmnI	GAANNNNTTC	1 cut(s)	1554
XspI	CTAG	3 cut(s)	228, 471, 1164
Zsp2I	ATGCAT	1 cut(s)	1690

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.