pycom15g32080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008452
Physical Location & Seq
Forward (+)
30958669 .. 30959319
651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32080.2

Sequence Viewer

Length: 444 bp
ATGAACAGTACAATTACTCTCCCAGATTCAGCCCCTATCATTTCATCTCCAACTATGGAAAAATCTGGTGACATAACTAGGGAATTATTCATTGATCCTGCGAGCCCTCACACCATTGAAAATGGTGCTCTGGTGACTGGCAATGGTGATAGCTCTGAAGATGTTCAGAAGTTTTCTGATACTGGCCCCAGTGCGGCAGTTTATCCGGACGAGGAAGAGGCTCGTTTCCTTCGATCTCTTGGTTGGGAGGAAAACTCTGGGGATGATGGTGGCCTTACGGAAGAAGAGATAAATGCATTTTATGACCAGTACATGAAATCACGACCATCTATGAAGTGCCGAGGCATGCAGCCAAAGCTTGCTGTGGTGTCTGAATCTCCTGTGACTGATTTGGGCAAGGCTCGGTCCGAATTGAGGTCAACTGACTCTGTGTCTGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

15.88

Weight (kDa)

4.32

Isoelectric Point (pI)

71.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 1 cut(s) 194
AclWI GGATC 1 cut(s) 89
AcuI CTGAAG 1 cut(s) 177
AfaI GTAC 2 cut(s) 10, 311
AfiI CCNNNNNNNGG 2 cut(s) 193, 414
AgsI TTSAA 1 cut(s) 119
AhdI GACNNNNNGTC 1 cut(s) 430
AluBI AGCT 3 cut(s) 153, 358, 440
AluI AGCT 3 cut(s) 153, 358, 440
Alw21I GWGCWC 1 cut(s) 130
AlwI GGATC 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 2 cut(s) 184, 271
ApeKI GCWGC 1 cut(s) 349
Asp700I GAANNNNTTC 1 cut(s) 162
AspS9I GGNCC 2 cut(s) 185, 405
AsuHPI GGTGA 3 cut(s) 80, 145, 158
AvaII GGWCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 1 cut(s) 361
BccI CCATC 2 cut(s) 260, 334
BfaI CTAG 1 cut(s) 78
BisI GCNGC 2 cut(s) 195, 350
BlsI GCNGC 2 cut(s) 196, 351
Bme18I GGWCC 1 cut(s) 405
BmeRI GACNNNNNGTC 1 cut(s) 430
BmgT120I GGNCC 2 cut(s) 185, 405
BmiI GGNNCC 1 cut(s) 187
BmrI ACTGGG 1 cut(s) 183
BmuI ACTGGG 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BsaJI CCNNGG 1 cut(s) 340
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 414
Bse1I ACTGG 4 cut(s) 142, 187, 189, 307
Bse3DI GCAATG 1 cut(s) 148
BseAI TCCGGA 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 340
BseGI GGATG 1 cut(s) 268
BseLI CCNNNNNNNGG 2 cut(s) 193, 414
BseMI GCAATG 1 cut(s) 148
BseNI ACTGG 4 cut(s) 142, 187, 189, 307
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 2 cut(s) 186, 273
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 193, 414
BsnI GGCC 2 cut(s) 186, 273
Bsp1286I GDGCHC 2 cut(s) 107, 130
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 2 cut(s) 94, 233
BspACI CCGC 1 cut(s) 194
BspANI GGCC 2 cut(s) 186, 273
BspEI TCCGGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 187
BspPI GGATC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 148
BsrI ACTGG 4 cut(s) 142, 187, 189, 307
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 2 cut(s) 94, 233
Bst4CI ACNGT 1 cut(s) 8
Bst6I CTCTTC 2 cut(s) 210, 279
BstC8I GCNNGC 3 cut(s) 103, 347, 360
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 2 cut(s) 97, 236
BstMBI GATC 2 cut(s) 94, 233
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 361
BsuRI GGCC 2 cut(s) 186, 273
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 3 cut(s) 103, 347, 360
Cfr13I GGNCC 2 cut(s) 185, 405
CpoI CGGWCCG 1 cut(s) 405
Csp6I GTAC 2 cut(s) 9, 310
CspI CGGWCCG 1 cut(s) 405
CviAII CATG 2 cut(s) 313, 346
CviQI GTAC 2 cut(s) 9, 310
DpnI GATC 2 cut(s) 96, 235
DpnII GATC 2 cut(s) 94, 233
DriI GACNNNNNGTC 1 cut(s) 430
Eam1104I CTCTTC 2 cut(s) 210, 279
Eam1105I GACNNNNNGTC 1 cut(s) 430
EarI CTCTTC 2 cut(s) 210, 279
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 405
Eco57I CTGAAG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 107
FaeI CATG 2 cut(s) 316, 349
FaiI YATR 6 cut(s) 56, 74, 303, 314, 332, 347
FatI CATG 2 cut(s) 312, 345
Fnu4HI GCNGC 2 cut(s) 195, 350
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 2 cut(s) 195, 350
FspBI CTAG 1 cut(s) 78
GluI GCNGC 2 cut(s) 195, 350
HaeIII GGCC 2 cut(s) 186, 273
HapII CCGG 1 cut(s) 206
Hin1II CATG 2 cut(s) 316, 349
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HindIII AAGCTT 2 cut(s) 356, 438
HinfI GANTC 3 cut(s) 26, 374, 425
HpaII CCGG 1 cut(s) 206
HphI GGTGA 3 cut(s) 80, 145, 158
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 6 cut(s) 157, 168, 178, 373, 409, 436
Hpy188III TCNNGA 2 cut(s) 206, 321
Hpy8I GTNNAC 1 cut(s) 420
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 1 cut(s) 8
HpyCH4V TGCA 2 cut(s) 296, 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
Hsp92II CATG 2 cut(s) 316, 349
Kpn2I TCCGGA 1 cut(s) 205
Kzo9I GATC 2 cut(s) 94, 233
Lsp1109I GCAGC 1 cut(s) 361
MaeI CTAG 1 cut(s) 78
MaeIII GTNAC 3 cut(s) 68, 133, 382
MalI GATC 2 cut(s) 96, 235
MboI GATC 2 cut(s) 94, 233
MboII GAAGA 4 cut(s) 170, 227, 293, 296
MhlI GDGCHC 2 cut(s) 107, 130
MluCI AATT 3 cut(s) 12, 83, 410
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 6 cut(s) 117, 205, 211, 241, 335, 408
Mph1103I ATGCAT 1 cut(s) 298
MroI TCCGGA 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 162
MspI CCGG 1 cut(s) 206
MwoI GCNNNNNNNGC 1 cut(s) 355
NdeII GATC 2 cut(s) 94, 233
NlaIII CATG 2 cut(s) 316, 349
NlaIV GGNNCC 1 cut(s) 187
NmeAIII GCCGAG 1 cut(s) 365
NmuCI GTSAC 3 cut(s) 68, 133, 382
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 1 cut(s) 349
PaeI GCATGC 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 2 cut(s) 26, 374
PkrI GCNGC 2 cut(s) 196, 351
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 2 cut(s) 185, 405
RsaI GTAC 2 cut(s) 10, 311
RsaNI GTAC 2 cut(s) 9, 310
Rsr2I CGGWCCG 1 cut(s) 405
RsrII CGGWCCG 1 cut(s) 405
SatI GCNGC 2 cut(s) 195, 350
Sau3AI GATC 2 cut(s) 94, 233
Sau96I GGNCC 2 cut(s) 185, 405
SchI GAGTC 1 cut(s) 419
SduI GDGCHC 2 cut(s) 107, 130
SetI ASST 4 cut(s) 155, 360, 419, 442
SinI GGWCC 1 cut(s) 405
SphI GCATGC 1 cut(s) 349
Sse9I AATT 3 cut(s) 12, 83, 410
SsiI CCGC 1 cut(s) 194
SspMI CTAG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 232
TaqII GACCGA 1 cut(s) 393
TasI AATT 3 cut(s) 12, 83, 410
TatI WGTACW 2 cut(s) 8, 309
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 2 cut(s) 26, 374
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 3 cut(s) 68, 133, 382
TseI GCWGC 1 cut(s) 349
Tsp45I GTSAC 3 cut(s) 68, 133, 382
TspDTI ATGAA 5 cut(s) 17, 33, 79, 329, 347
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 405
XceI RCATGY 1 cut(s) 349
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.