MD08G1182400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012040
Physical Location & Seq
Forward (+)
22637132 .. 22638255
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1182400.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGTTTCTTTTCAAGCTCATCCTCTTTCTCCAATCCAAAAGAAGGCAATCCCAGACTTTGAGAATTCATCCAAGAAAAGAAAATGGGACGACGATCCATACGATCATACGGAGCAACTTTTCGAAAAACGTGTGAAAGTACCACAAAGCACACCGAAATCCTTGTTTGATATCGAGCTTCACCTTGAGACTCCATTGCCTTTGGAGTGGCAAAGATGCCTTGATATTCAGTCGGGGCAGATACATTTTTACAACACAAGGACTCAGATGAAGACTTCAAGGGACCCAAGGAGGAGCCCTGAGCCACCAACTAGTCCAGCTGCTGATCATCATCGTCGTCGTCGTCGTCCCATGAGCCTCGACCTGGAGCTCAACCTAAGATCATGTGATCAATCCAACGCTAATTCAGACATTTCATCAACACACAACTTCAGTGCTTTGTTCATGGAACCCCACAGACCAACAAAAAGTAACCCTATTAATTTAGGGTTTCATGTTGATCATCACCATCAGCAGCAGCAGGAGATGGTGGCCACTGTGTGCATCAAGTGCCACATGCTGGTGATGCTGTGCAGGTCTTCTCCTGCATGCCCTAACTGCAAGTACATGCACCAACTTGATCAGAGCCCCCCAAACCTATTCAATTTCAAGCCAAGGCGTACTAGTAGCTTCATGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

26.4

Weight (kDa)

8.94

Isoelectric Point (pI)

83.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012040)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22250 AT1G78170 AT4G08910
fragaria_vesca FvH4_5g37370
malus_domestica MD08G1182400.v1.1 MD15G1368100.v1.1
prunus_persica Prupe.1G516300_v2.0.a1
pyrus_communis pycom08g15620 pycom15g33000
rosa_chinensis RchiOBHm_Chr7g0239041
rosa_laevigata RLG00000000788
rosa_multiflora Rmu_sc0005651.1_g000002
rosa_roxburghii Rroxscaffold_3G00221340
rosa_rugosa Rorug07G0324800
rosa_samantha Rh7AG479700 Rh7BG452500 Rh7CG497200 Rh7DG464700
rosa_wichuraiana Rw7G040110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 564
AccB7I CCANNNNNTGG 1 cut(s) 559
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 1 cut(s) 415
AdeI CACNNNGTG 1 cut(s) 540
AfaI GTAC 3 cut(s) 141, 605, 661
AfiI CCNNNNNNNGG 3 cut(s) 43, 364, 559
AflIII ACRYGT 1 cut(s) 130
AgsI TTSAA 4 cut(s) 14, 279, 643, 649
AhlI ACTAGT 2 cut(s) 311, 662
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 5 cut(s) 17, 178, 320, 370, 669
AluI AGCT 5 cut(s) 17, 178, 320, 370, 669
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 89
AlwNI CAGNNNCTG 1 cut(s) 323
AoxI GGCC 1 cut(s) 531
ApeKI GCWGC 3 cut(s) 320, 514, 517
ApoI RAATTY 1 cut(s) 64
AseI ATTAAT 1 cut(s) 480
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 3 cut(s) 173, 497, 574
AsuII TTCGAA 1 cut(s) 123
AvaII GGWCC 1 cut(s) 283
BalI TGGCCA 1 cut(s) 533
BanII GRGCYC 3 cut(s) 299, 372, 629
BbsI GAAGAC 2 cut(s) 278, 570
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 3 cut(s) 307, 526, 529
BccI CCATC 2 cut(s) 516, 520
BciT130I CCWGG 1 cut(s) 365
BclI TGATCA 4 cut(s) 325, 388, 499, 619
BcoDI GTCTC 1 cut(s) 182
BcuI ACTAGT 2 cut(s) 311, 662
BfaI CTAG 2 cut(s) 312, 663
BfuAI ACCTGC 1 cut(s) 564
BisI GCNGC 3 cut(s) 321, 515, 518
BlsI GCNGC 3 cut(s) 322, 516, 519
Bme1390I CCNGG 1 cut(s) 365
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 4 cut(s) 284, 285, 296, 450
BmrFI CCNGG 1 cut(s) 365
BmsI GCATC 3 cut(s) 206, 552, 555
BpiI GAAGAC 2 cut(s) 278, 570
BpmI CTGGAG 1 cut(s) 386
Bpu10I CCTNAGC 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 330
BsaJI CCNNGG 2 cut(s) 287, 653
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 364, 559
Bse3DI GCAATG 1 cut(s) 194
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 2 cut(s) 287, 653
BseGI GGATG 2 cut(s) 19, 68
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 43, 364, 559
BseMI GCAATG 1 cut(s) 194
BseMII CTCAG 2 cut(s) 278, 291
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 3 cut(s) 307, 526, 529
BsgI GTGCAG 1 cut(s) 592
BshFI GGCC 1 cut(s) 533
BsiHKAI GWGCWC 1 cut(s) 372
BslFI GGGAC 3 cut(s) 101, 296, 333
BslI CCNNNNNNNGG 3 cut(s) 43, 364, 559
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 3 cut(s) 101, 296, 333
BsnI GGCC 1 cut(s) 533
Bsp119I TTCGAA 1 cut(s) 123
Bsp1286I GDGCHC 3 cut(s) 299, 372, 629
Bsp143I GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 277, 292
BspLI GGNNCC 4 cut(s) 284, 285, 296, 450
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 194
BssECI CCNNGG 2 cut(s) 287, 653
BssMI GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
BssT1I CCWWGG 2 cut(s) 287, 653
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 1 cut(s) 538
BstAPI GCANNNNNTGC 1 cut(s) 549
BstBI TTCGAA 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 589
BstDEI CTNAG 3 cut(s) 264, 300, 377
BstF5I GGATG 2 cut(s) 19, 68
BstKTI GATC 7 cut(s) 97, 106, 328, 383, 391, 502, 622
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
BstMWI GCNNNNNNNGC 3 cut(s) 549, 565, 597
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 3 cut(s) 559, 591, 610
BstSCI CCNGG 1 cut(s) 363
BstV1I GCAGC 3 cut(s) 307, 526, 529
BstV2I GAAGAC 2 cut(s) 278, 570
BsuRI GGCC 1 cut(s) 533
BtsCI GGATG 2 cut(s) 19, 68
BtsIMutI CAGTG 2 cut(s) 439, 534
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 589
CaiI CAGNNNCTG 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 283
Csp6I GTAC 3 cut(s) 140, 604, 660
CviAII CATG 8 cut(s) 352, 384, 445, 494, 556, 588, 607, 673
CviQI GTAC 3 cut(s) 140, 604, 660
DdeI CTNAG 3 cut(s) 264, 300, 377
DpnI GATC 7 cut(s) 96, 105, 327, 382, 390, 501, 621
DpnII GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
DraIII CACNNNGTG 1 cut(s) 540
EaeI YGGCCR 1 cut(s) 531
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 2 cut(s) 287, 653
Eco24I GRGCYC 3 cut(s) 299, 372, 629
Eco32I GATATC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 1 cut(s) 415
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 283
EcoRI GAATTC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 363
EcoRV GATATC 1 cut(s) 172
EcoT14I CCWWGG 2 cut(s) 287, 653
EcoT38I GRGCYC 3 cut(s) 299, 372, 629
ErhI CCWWGG 2 cut(s) 287, 653
FaeI CATG 8 cut(s) 355, 387, 448, 497, 559, 591, 610, 676
FaqI GGGAC 3 cut(s) 101, 296, 333
FatI CATG 8 cut(s) 351, 383, 444, 493, 555, 587, 606, 672
FbaI TGATCA 4 cut(s) 325, 388, 499, 619
Fnu4HI GCNGC 3 cut(s) 321, 515, 518
FokI GGATG 2 cut(s) 6, 55
FriOI GRGCYC 3 cut(s) 299, 372, 629
Fsp4HI GCNGC 3 cut(s) 321, 515, 518
FspBI CTAG 2 cut(s) 312, 663
GluI GCNGC 3 cut(s) 321, 515, 518
GsuI CTGGAG 1 cut(s) 386
HaeIII GGCC 1 cut(s) 533
Hin1II CATG 8 cut(s) 355, 387, 448, 497, 559, 591, 610, 676
HinfI GANTC 2 cut(s) 190, 262
HphI GGTGA 3 cut(s) 173, 497, 574
Hpy188I TCNGA 3 cut(s) 267, 409, 624
Hpy99I CGWCG 5 cut(s) 95, 339, 342, 345, 348
HpyAV CCTTC 1 cut(s) 37
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 5 cut(s) 543, 573, 587, 600, 610
HpyF10VI GCNNNNNNNGC 3 cut(s) 549, 565, 597
HpyF3I CTNAG 3 cut(s) 264, 300, 377
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 8 cut(s) 355, 387, 448, 497, 559, 591, 610, 676
KflI GGGWCCC 1 cut(s) 283
Ksp22I TGATCA 4 cut(s) 325, 388, 499, 619
Kzo9I GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
LmnI GCTCC 3 cut(s) 112, 294, 367
LpnPI CCDG 9 cut(s) 66, 312, 330, 350, 377, 506, 545, 559, 597
Lsp1109I GCAGC 3 cut(s) 307, 526, 529
LweI GCATC 3 cut(s) 206, 552, 555
MaeI CTAG 2 cut(s) 312, 663
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 1 cut(s) 470
MalI GATC 7 cut(s) 96, 105, 327, 382, 390, 501, 621
MboI GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
MboII GAAGA 2 cut(s) 283, 570
MhlI GDGCHC 3 cut(s) 299, 372, 629
MlsI TGGCCA 1 cut(s) 533
MluCI AATT 4 cut(s) 64, 403, 481, 643
MluNI TGGCCA 1 cut(s) 533
MlyI GAGTC 2 cut(s) 184, 256
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 3 cut(s) 33, 285, 368
Mox20I TGGCCA 1 cut(s) 533
MscI TGGCCA 1 cut(s) 533
MseI TTAA 2 cut(s) 480, 679
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 365
MwoI GCNNNNNNNGC 3 cut(s) 549, 565, 597
NdeII GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
NlaIII CATG 8 cut(s) 355, 387, 448, 497, 559, 591, 610, 676
NlaIV GGNNCC 4 cut(s) 284, 285, 296, 450
NspI RCATGY 3 cut(s) 559, 591, 610
NspV TTCGAA 1 cut(s) 123
PaeI GCATGC 1 cut(s) 591
PcsI WCGNNNNNNNCGW 3 cut(s) 99, 340, 343
PflMI CCANNNNNTGG 1 cut(s) 559
PkrI GCNGC 3 cut(s) 322, 516, 519
PleI GAGTC 2 cut(s) 184, 256
PpsI GAGTC 2 cut(s) 184, 256
PpuMI RGGWCCY 1 cut(s) 283
PshBI ATTAAT 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 4 cut(s) 284, 285, 296, 450
PspPI GGNCC 1 cut(s) 283
PspPPI RGGWCCY 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 3 cut(s) 141, 605, 661
RsaNI GTAC 3 cut(s) 140, 604, 660
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 1 cut(s) 372
SaqAI TTAA 2 cut(s) 480, 679
SatI GCNGC 3 cut(s) 321, 515, 518
Sau3AI GATC 7 cut(s) 94, 103, 325, 380, 388, 499, 619
Sau96I GGNCC 1 cut(s) 283
SchI GAGTC 2 cut(s) 184, 256
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 3 cut(s) 299, 372, 629
SfaNI GCATC 3 cut(s) 206, 552, 555
SfuI TTCGAA 1 cut(s) 123
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
SpeI ACTAGT 2 cut(s) 311, 662
SphI GCATGC 1 cut(s) 591
Sse9I AATT 4 cut(s) 64, 403, 481, 643
SspMI CTAG 2 cut(s) 312, 663
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 2 cut(s) 287, 653
TaaI ACNGT 1 cut(s) 538
TaiI ACGT 1 cut(s) 133
TaqI TCGA 3 cut(s) 123, 174, 360
TasI AATT 4 cut(s) 64, 403, 481, 643
TatI WGTACW 1 cut(s) 603
Tru1I TTAA 2 cut(s) 480, 679
Tru9I TTAA 2 cut(s) 480, 679
TscAI CASTG 2 cut(s) 439, 541
TseI GCWGC 3 cut(s) 320, 514, 517
TspDTI ATGAA 6 cut(s) 57, 284, 405, 433, 482, 661
TspGWI ACGGA 1 cut(s) 125
TspRI CASTG 2 cut(s) 439, 541
Van91I CCANNNNNTGG 1 cut(s) 559
VpaK11BI GGWCC 1 cut(s) 283
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 64
XceI RCATGY 3 cut(s) 559, 591, 610
XspI CTAG 2 cut(s) 312, 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.