Rorug07G0324800

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000742
Physical Location & Seq
Forward (+)
33700889 .. 33704547
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0324800.1

Sequence Viewer

Length: 1170 bp
ATGTCCCATCGCAAGTTTGAACACCCAAGGCATGGCTCTCTCGGATTTCTTCCAAGGAAAAGAGCATCCCGCCACAGAGGAAAAGTGAAGGCATTCCCCAGAGATAATCCAATGACATCTTGCAGATTGACTGCTTTCCTGGGATACAAAGCAGGAATGACCCATATTGTTAGAGATGTTGAAAAGCCAGGATCAAAGCTACACAAAAAGGAAACATGTGAAGCAGTTACAGTTATTGAAACACCACCCATGGTGGTTGTTGGGGTTGTGGGTTATGTGAAGACACCCCGTGGTCTCCGTTCTTTGGACACAGTTTGGGCCCAGCACCTGAGTGAGGAGGTGAAGAGAAGATTCTACAAGAACTGGTGTAAGTCTAAGAAGAAGGCATTCACTAAGCACTCGAACAAATACGAGAGTGAAAATGGGAAAAGGGACATCCACGCCCAATTGGAGAAAATGAAAAAGTACTGCTCTGTGATTCGTGTTTTAGCCCACACTCAGATTAGAAAAATGAAGGGACTGAAGCAGAAGAAAGCCCATTTGATGGAGATTCAGGTGAATGGTGGAGATATTGCAAAGAAGGTTGACTATGCTTACAGCTTCTTTGAGAAGCAGATTCCTGTTGATGCTGTTTTCCAGAAAGATGAGATGATTGACATTATTGGGGTGACAAAGGGAAAAGGCTATGAGGGTGTGGTCACTCGCTGGGGTGTCACTCGTCTTCCCCGTAAGACCCATCGTGGGCTTAGAAAAGTAGCCTGCATTGGTGCTTGGCATCCAGCTAGGGTTTCGTTTACTGTTGCCAGGGCTGGGCAGAATGGCTACCATCATCGTACTGAGATGAACAAGAAGATATACAAGCTTGGAAAGTCTGGGCAGGAGTCTCACTCTGCAATGACCGAGTTTGACAGGACGGAGAAGGATATTACGCCCATGGGTGGTTTCCCACATTATGGTGGGGTGAAAGAGGATTATCTTGTCATAAAGGGCTGCTGTGTAGGTCCGAAAAAGCGAGTTGTTACCTTGAGGCAGTCACTTTTGAAGCAGACATCACGGGTTGCCATGGAGGAGATAAAGCTCAAGTTCATTGATACGTCATCCAAATTTGGCCATGGTTGTTTCCAAACTACACAAGAGAAGGCAAGGTTCTATGGCCGGCTCAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Exo_endo_phos zf-C6H2

Protein Analysis

389

Amino Acids

44.45

Weight (kDa)

10.13

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L3 PF00297 1 - 370 6.5e-193 Ribosomal protein L3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 32, 544, 953
AciI CCGC 1 cut(s) 70
AclWI GGATC 1 cut(s) 199
AcoI YGGCCR 2 cut(s) 1108, 1153
AcsI RAATTY 1 cut(s) 1103
AcuI CTGAAG 1 cut(s) 542
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 2 cut(s) 467, 835
AfiI CCNNNNNNNGG 9 cut(s) 32, 304, 334, 544, 741, 810, 938, 953, 1162
AflIII ACRYGT 1 cut(s) 215
AgsI TTSAA 4 cut(s) 20, 182, 239, 1042
AjnI CCWGG 3 cut(s) 138, 187, 803
AleI CACNNNNGTG 1 cut(s) 330
AluBI AGCT 5 cut(s) 199, 600, 782, 862, 1078
AluI AGCT 5 cut(s) 199, 600, 782, 862, 1078
Alw26I GTCTC 2 cut(s) 299, 888
AlwI GGATC 1 cut(s) 199
AlwNI CAGNNNCTG 1 cut(s) 328
AoxI GGCC 3 cut(s) 318, 1108, 1153
ApaI GGGCCC 1 cut(s) 322
ApeKI GCWGC 1 cut(s) 990
ApoI RAATTY 1 cut(s) 1103
Asp700I GAANNNNTTC 2 cut(s) 92, 386
AspS9I GGNCC 3 cut(s) 318, 319, 1001
AsuHPI GGTGA 4 cut(s) 352, 568, 679, 973
AvaII GGWCC 1 cut(s) 1001
BaeGI GKGCMC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 1110
BanII GRGCYC 1 cut(s) 322
BbsI GAAGAC 2 cut(s) 287, 713
BbvI GCAGC 1 cut(s) 977
BccI CCATC 4 cut(s) 15, 538, 744, 834
BciT130I CCWGG 3 cut(s) 140, 189, 805
BciVI GTATCC 1 cut(s) 137
BcoDI GTCTC 2 cut(s) 299, 888
BfaI CTAG 1 cut(s) 783
BfuI GTATCC 1 cut(s) 137
BisI GCNGC 1 cut(s) 991
BlsI GCNGC 1 cut(s) 992
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 3 cut(s) 140, 189, 805
Bme18I GGWCC 1 cut(s) 1001
BmgT120I GGNCC 3 cut(s) 318, 319, 1001
BmiI GGNNCC 1 cut(s) 320
BmrFI CCNGG 3 cut(s) 140, 189, 805
BmsI GCATC 3 cut(s) 74, 616, 784
BpiI GAAGAC 2 cut(s) 287, 713
BplI GAGNNNNNCTC 2 cut(s) 872, 904
BpuEI CTTGAG 3 cut(s) 1045, 1064, 1145
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 9 cut(s) 26, 53, 139, 249, 289, 804, 933, 1062, 1111
Bsc4I CCNNNNNNNGG 9 cut(s) 32, 304, 334, 544, 741, 810, 938, 953, 1162
Bse118I RCCGGY 1 cut(s) 1155
Bse1I ACTGG 1 cut(s) 368
Bse3DI GCAATG 1 cut(s) 900
BseBI CCWGG 3 cut(s) 140, 189, 805
BseDI CCNNGG 9 cut(s) 26, 53, 139, 249, 289, 804, 933, 1062, 1111
BseGI GGATG 4 cut(s) 65, 435, 775, 1097
BseLI CCNNNNNNNGG 9 cut(s) 32, 304, 334, 544, 741, 810, 938, 953, 1162
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 3 cut(s) 320, 512, 828
BseNI ACTGG 1 cut(s) 368
BseRI GAGGAG 2 cut(s) 350, 1082
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 1 cut(s) 977
BseYI CCCAGC 3 cut(s) 321, 705, 809
BshFI GGCC 3 cut(s) 320, 1110, 1155
BsiSI CCGG 1 cut(s) 1156
BslFI GGGAC 2 cut(s) 446, 531
BslI CCNNNNNNNGG 9 cut(s) 32, 304, 334, 544, 741, 810, 938, 953, 1162
BsmAI GTCTC 2 cut(s) 299, 888
BsmFI GGGAC 2 cut(s) 446, 531
BsmI GAATGC 2 cut(s) 92, 386
BsnI GGCC 3 cut(s) 320, 1110, 1155
Bso31I GGTCTC 1 cut(s) 299
Bsp120I GGGCCC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 1 cut(s) 191
Bsp19I CCATGG 4 cut(s) 249, 933, 1062, 1111
BspACI CCGC 1 cut(s) 70
BspANI GGCC 3 cut(s) 320, 1110, 1155
BspCNI CTCAG 3 cut(s) 321, 511, 829
BspLI GGNNCC 1 cut(s) 320
BspPI GGATC 1 cut(s) 199
BspTNI GGTCTC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 900
BsrFI RCCGGY 1 cut(s) 1155
BsrI ACTGG 1 cut(s) 368
BssAI RCCGGY 1 cut(s) 1155
BssECI CCNNGG 9 cut(s) 26, 53, 139, 249, 289, 804, 933, 1062, 1111
BssMI GATC 1 cut(s) 191
BssT1I CCWWGG 6 cut(s) 26, 53, 249, 933, 1062, 1111
Bst2UI CCWGG 3 cut(s) 140, 189, 805
Bst4CI ACNGT 3 cut(s) 232, 313, 799
Bst6I CTCTTC 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 760, 1157
BstDEI CTNAG 6 cut(s) 329, 375, 393, 498, 746, 837
BstDSI CCRYGG 5 cut(s) 249, 289, 933, 1062, 1111
BstENI CCTNNNNNAGG 1 cut(s) 332
BstF5I GGATG 4 cut(s) 65, 435, 775, 1097
BstKTI GATC 1 cut(s) 194
BstMAI GTCTC 2 cut(s) 299, 888
BstMBI GATC 1 cut(s) 191
BstNI CCWGG 3 cut(s) 140, 189, 805
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 3 cut(s) 138, 187, 803
BstSLI GKGCMC 1 cut(s) 322
BstV1I GCAGC 1 cut(s) 977
BstV2I GAAGAC 2 cut(s) 287, 713
BsuI GTATCC 1 cut(s) 137
BsuRI GGCC 3 cut(s) 320, 1110, 1155
BtgI CCRYGG 5 cut(s) 249, 289, 933, 1062, 1111
BtsCI GGATG 4 cut(s) 65, 435, 775, 1097
Cac8I GCNNGC 2 cut(s) 760, 1157
CaiI CAGNNNCTG 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 1155
Cfr13I GGNCC 3 cut(s) 318, 319, 1001
Csp6I GTAC 2 cut(s) 466, 834
CviAII CATG 6 cut(s) 32, 216, 250, 934, 1063, 1112
CviQI GTAC 2 cut(s) 466, 834
DdeI CTNAG 6 cut(s) 329, 375, 393, 498, 746, 837
DpnI GATC 1 cut(s) 193
DpnII GATC 1 cut(s) 191
DraIII CACNNNGTG 1 cut(s) 290
EaeI YGGCCR 2 cut(s) 1108, 1153
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco130I CCWWGG 6 cut(s) 26, 53, 249, 933, 1062, 1111
Eco24I GRGCYC 1 cut(s) 322
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 1001
Eco57I CTGAAG 1 cut(s) 542
EcoNI CCTNNNNNAGG 1 cut(s) 332
EcoRII CCWGG 3 cut(s) 138, 187, 803
EcoT14I CCWWGG 6 cut(s) 26, 53, 249, 933, 1062, 1111
EcoT38I GRGCYC 1 cut(s) 322
ErhI CCWWGG 6 cut(s) 26, 53, 249, 933, 1062, 1111
FaeI CATG 6 cut(s) 35, 219, 253, 937, 1066, 1115
FaqI GGGAC 2 cut(s) 446, 531
FatI CATG 6 cut(s) 31, 215, 249, 933, 1062, 1111
FauI CCCGC 1 cut(s) 77
Fnu4HI GCNGC 1 cut(s) 991
FokI GGATG 4 cut(s) 52, 422, 762, 1084
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 1 cut(s) 991
FspBI CTAG 1 cut(s) 783
GluI GCNGC 1 cut(s) 991
GsaI CCCAGC 3 cut(s) 325, 709, 813
HaeIII GGCC 3 cut(s) 320, 1110, 1155
HapII CCGG 1 cut(s) 1156
Hin1II CATG 6 cut(s) 35, 219, 253, 937, 1066, 1115
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 5 cut(s) 351, 478, 550, 616, 881
HpaII CCGG 1 cut(s) 1156
HphI GGTGA 4 cut(s) 352, 568, 679, 973
Hpy166II GTNNAC 2 cut(s) 586, 795
Hpy188I TCNGA 3 cut(s) 44, 501, 1005
Hpy188III TCNNGA 1 cut(s) 637
Hpy8I GTNNAC 2 cut(s) 586, 795
HpyAV CCTTC 6 cut(s) 82, 376, 508, 574, 913, 1132
HpyCH4III ACNGT 3 cut(s) 232, 313, 799
HpyCH4IV ACGT 1 cut(s) 1094
HpyCH4V TGCA 4 cut(s) 123, 575, 762, 893
HpyF3I CTNAG 6 cut(s) 329, 375, 393, 498, 746, 837
HpySE526I ACGT 1 cut(s) 1094
Hsp92II CATG 6 cut(s) 35, 219, 253, 937, 1066, 1115
KroI GCCGGC 1 cut(s) 1155
KroNI GCCGGC 1 cut(s) 1157
Kzo9I GATC 1 cut(s) 191
Lsp1109I GCAGC 1 cut(s) 977
LweI GCATC 3 cut(s) 74, 616, 784
MaeI CTAG 1 cut(s) 783
MaeII ACGT 1 cut(s) 1094
MaeIII GTNAC 6 cut(s) 226, 667, 697, 712, 1018, 1032
MalI GATC 1 cut(s) 193
MboI GATC 1 cut(s) 191
MboII GAAGA 8 cut(s) 41, 292, 355, 360, 391, 541, 713, 862
MfeI CAATTG 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 322
MlsI TGGCCA 1 cut(s) 1110
MluCI AATT 2 cut(s) 446, 1103
MluNI TGGCCA 1 cut(s) 1110
MlyI GAGTC 1 cut(s) 890
MnlI CCTC 7 cut(s) 71, 328, 331, 682, 961, 1020, 1060
Mox20I TGGCCA 1 cut(s) 1110
MroNI GCCGGC 1 cut(s) 1155
MroXI GAANNNNTTC 2 cut(s) 92, 386
MscI TGGCCA 1 cut(s) 1110
MslI CAYNNNNRTG 2 cut(s) 330, 954
Msp20I TGGCCA 1 cut(s) 1110
MspI CCGG 1 cut(s) 1156
MspR9I CCNGG 3 cut(s) 140, 189, 805
MunI CAATTG 1 cut(s) 446
Mva1269I GAATGC 2 cut(s) 92, 386
MvaI CCWGG 3 cut(s) 140, 189, 805
NaeI GCCGGC 1 cut(s) 1157
NcoI CCATGG 4 cut(s) 249, 933, 1062, 1111
NdeII GATC 1 cut(s) 191
NgoMIV GCCGGC 1 cut(s) 1155
NlaIII CATG 6 cut(s) 35, 219, 253, 937, 1066, 1115
NlaIV GGNNCC 1 cut(s) 320
NmuCI GTSAC 4 cut(s) 667, 697, 712, 1032
NspI RCATGY 1 cut(s) 219
OliI CACNNNNGTG 1 cut(s) 330
PciI ACATGT 1 cut(s) 215
PcsI WCGNNNNNNNCGW 1 cut(s) 724
PctI GAATGC 2 cut(s) 92, 386
PdiI GCCGGC 1 cut(s) 1157
PdmI GAANNNNTTC 2 cut(s) 92, 386
PfeI GAWTC 4 cut(s) 351, 478, 550, 616
PflMI CCANNNNNTGG 3 cut(s) 32, 544, 953
PkrI GCNGC 1 cut(s) 992
PleI GAGTC 1 cut(s) 889
PpsI GAGTC 1 cut(s) 889
PscI ACATGT 1 cut(s) 215
Psp6I CCWGG 3 cut(s) 138, 187, 803
PspFI CCCAGC 3 cut(s) 321, 705, 809
PspGI CCWGG 3 cut(s) 138, 187, 803
PspN4I GGNNCC 1 cut(s) 320
PspOMI GGGCCC 1 cut(s) 318
PspPI GGNCC 3 cut(s) 318, 319, 1001
PstNI CAGNNNCTG 1 cut(s) 328
RsaI GTAC 2 cut(s) 467, 835
RsaNI GTAC 2 cut(s) 466, 834
RseI CAYNNNNRTG 2 cut(s) 330, 954
SatI GCNGC 1 cut(s) 991
Sau3AI GATC 1 cut(s) 191
Sau96I GGNCC 3 cut(s) 318, 319, 1001
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 1 cut(s) 890
ScrFI CCNGG 3 cut(s) 140, 189, 805
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 3 cut(s) 74, 616, 784
SinI GGWCC 1 cut(s) 1001
SmiMI CAYNNNNRTG 2 cut(s) 330, 954
SmlI CTYRAG 3 cut(s) 1024, 1079, 1160
SmoI CTYRAG 3 cut(s) 1024, 1079, 1160
Sse9I AATT 2 cut(s) 446, 1103
SsiI CCGC 1 cut(s) 70
SspMI CTAG 1 cut(s) 783
StyD4I CCNGG 3 cut(s) 138, 187, 803
StyI CCWWGG 6 cut(s) 26, 53, 249, 933, 1062, 1111
TaaI ACNGT 3 cut(s) 232, 313, 799
TaiI ACGT 1 cut(s) 1097
TaqI TCGA 1 cut(s) 401
TaqII GACCGA 1 cut(s) 914
TasI AATT 2 cut(s) 446, 1103
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 4 cut(s) 351, 478, 550, 616
TseFI GTSAC 4 cut(s) 667, 697, 712, 1032
TseI GCWGC 1 cut(s) 990
Tsp45I GTSAC 4 cut(s) 667, 697, 712, 1032
TspDTI ATGAA 4 cut(s) 473, 527, 857, 1075
TspGWI ACGGA 2 cut(s) 287, 929
Van91I CCANNNNNTGG 3 cut(s) 32, 544, 953
VpaK11BI GGWCC 1 cut(s) 1001
XagI CCTNNNNNAGG 1 cut(s) 332
XapI RAATTY 1 cut(s) 1103
XceI RCATGY 1 cut(s) 219
XmnI GAANNNNTTC 2 cut(s) 92, 386
XspI CTAG 1 cut(s) 783
ZrmI AGTACT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.