Synaptonemal complex protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 2821 |
| Acc36I | ACCTGC | 1 cut(s) | 2821 |
| AccB1I | GGYRCC | 2 cut(s) | 2567, 2664 |
| AccII | CGCG | 2 cut(s) | 103, 2602 |
| AccIII | TCCGGA | 1 cut(s) | 1710 |
| AciI | CCGC | 8 cut(s) | 107, 153, 2562, 2597, 2600, 2618, 2621, 2753 |
| AclWI | GGATC | 5 cut(s) | 45, 1945, 2453, 2466, 2672 |
| AcoI | YGGCCR | 1 cut(s) | 2757 |
| AcsI | RAATTY | 4 cut(s) | 1131, 1915, 2170, 2335 |
| AcuI | CTGAAG | 7 cut(s) | 1109, 1233, 1445, 1871, 2021, 2048, 2307 |
| AcyI | GRCGYC | 1 cut(s) | 2594 |
| AdeI | CACNNNGTG | 1 cut(s) | 2345 |
| AfaI | GTAC | 2 cut(s) | 1174, 2485 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1185, 2279, 2374, 2873 |
| AgeI | ACCGGT | 1 cut(s) | 2405 |
| AjnI | CCWGG | 2 cut(s) | 408, 2504 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 695, 727 |
| AleI | CACNNNNGTG | 1 cut(s) | 240 |
| Alw21I | GWGCWC | 1 cut(s) | 237 |
| Alw26I | GTCTC | 5 cut(s) | 70, 575, 1018, 1228, 1776 |
| AlwI | GGATC | 5 cut(s) | 45, 1945, 2453, 2466, 2672 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2731 |
| Ama87I | CYCGRG | 1 cut(s) | 569 |
| Aor13HI | TCCGGA | 1 cut(s) | 1710 |
| AoxI | GGCC | 4 cut(s) | 117, 2520, 2643, 2757 |
| ApeKI | GCWGC | 8 cut(s) | 893, 1447, 1586, 1837, 1868, 2604, 2731, 2816 |
| ApoI | RAATTY | 4 cut(s) | 1131, 1915, 2170, 2335 |
| AseI | ATTAAT | 1 cut(s) | 1701 |
| AsiGI | ACCGGT | 1 cut(s) | 2405 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 86, 269 |
| AspLEI | GCGC | 4 cut(s) | 1873, 2604, 2887, 2911 |
| AspS9I | GGNCC | 4 cut(s) | 74, 2077, 2521, 2644 |
| AsuC2I | CCSGG | 1 cut(s) | 2874 |
| AsuHPI | GGTGA | 7 cut(s) | 142, 728, 1445, 2262, 2331, 2618, 2893 |
| AsuII | TTCGAA | 1 cut(s) | 2174 |
| AvaI | CYCGRG | 1 cut(s) | 569 |
| AvaII | GGWCC | 2 cut(s) | 74, 2077 |
| BaeGI | GKGCMC | 1 cut(s) | 2669 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1181, 1214 |
| BamHI | GGATCC | 1 cut(s) | 2458 |
| BanI | GGYRCC | 2 cut(s) | 2567, 2664 |
| BbsI | GAAGAC | 3 cut(s) | 89, 1877, 2251 |
| Bbv12I | GWGCWC | 1 cut(s) | 237 |
| BbvI | GCAGC | 8 cut(s) | 880, 1459, 1573, 1849, 1855, 2616, 2718, 2828 |
| BccI | CCATC | 6 cut(s) | 223, 467, 816, 1409, 1456, 2768 |
| BceAI | ACGGC | 2 cut(s) | 2647, 2872 |
| BciT130I | CCWGG | 2 cut(s) | 410, 2506 |
| BciVI | GTATCC | 1 cut(s) | 1182 |
| BclI | TGATCA | 3 cut(s) | 367, 1033, 1690 |
| BcnI | CCSGG | 1 cut(s) | 2874 |
| BcoDI | GTCTC | 5 cut(s) | 70, 575, 1018, 1228, 1776 |
| BfmI | CTRYAG | 3 cut(s) | 690, 2732, 2814 |
| BfoI | RGCGCY | 1 cut(s) | 2888 |
| BfuAI | ACCTGC | 1 cut(s) | 2821 |
| BfuI | GTATCC | 1 cut(s) | 1182 |
| BglII | AGATCT | 3 cut(s) | 193, 1000, 2699 |
| BlpI | GCTNAGC | 1 cut(s) | 1011 |
| Bme1390I | CCNGG | 3 cut(s) | 410, 2506, 2874 |
| Bme18I | GGWCC | 2 cut(s) | 74, 2077 |
| BmeT110I | CYCGRG | 1 cut(s) | 569 |
| BmgT120I | GGNCC | 4 cut(s) | 74, 2077, 2521, 2644 |
| BmiI | GGNNCC | 4 cut(s) | 2460, 2560, 2569, 2666 |
| BmrFI | CCNGG | 3 cut(s) | 410, 2506, 2874 |
| BmrI | ACTGGG | 1 cut(s) | 2719 |
| BmsI | GCATC | 8 cut(s) | 403, 733, 1066, 1583, 1836, 2557, 2632, 2886 |
| BmuI | ACTGGG | 1 cut(s) | 2719 |
| BpiI | GAAGAC | 3 cut(s) | 89, 1877, 2251 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 104, 136 |
| BpmI | CTGGAG | 3 cut(s) | 575, 1757, 2577 |
| Bpu10I | CCTNAGC | 1 cut(s) | 774 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1011 |
| Bpu14I | TTCGAA | 1 cut(s) | 2174 |
| BpuEI | CTTGAG | 3 cut(s) | 749, 875, 1655 |
| BpuMI | CCSGG | 1 cut(s) | 2874 |
| Bsa29I | ATCGAT | 2 cut(s) | 227, 2551 |
| BsaBI | GATNNNNATC | 2 cut(s) | 807, 2698 |
| BsaHI | GRCGYC | 1 cut(s) | 2594 |
| BsaI | GGTCTC | 2 cut(s) | 70, 1228 |
| BsaJI | CCNNGG | 3 cut(s) | 2504, 2629, 2872 |
| BsaWI | WCCGGW | 3 cut(s) | 1710, 2248, 2405 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1185, 2279, 2374, 2873 |
| Bse118I | RCCGGY | 3 cut(s) | 2405, 2564, 2755 |
| Bse1I | ACTGG | 3 cut(s) | 979, 2297, 2725 |
| Bse8I | GATNNNNATC | 2 cut(s) | 807, 2698 |
| BseAI | TCCGGA | 1 cut(s) | 1710 |
| BseBI | CCWGG | 2 cut(s) | 410, 2506 |
| BseCI | ATCGAT | 2 cut(s) | 227, 2551 |
| BseDI | CCNNGG | 3 cut(s) | 2504, 2629, 2872 |
| BseGI | GGATG | 7 cut(s) | 237, 418, 754, 1574, 1707, 2760, 2877 |
| BseJI | GATNNNNATC | 2 cut(s) | 807, 2698 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1185, 2279, 2374, 2873 |
| BseMII | CTCAG | 9 cut(s) | 81, 408, 788, 1034, 1296, 1344, 1353, 2012, 2717 |
| BseNI | ACTGG | 3 cut(s) | 979, 2297, 2725 |
| BseRI | GAGGAG | 5 cut(s) | 43, 593, 2111, 2168, 2171 |
| BseSI | GKGCMC | 1 cut(s) | 2669 |
| BseXI | GCAGC | 8 cut(s) | 880, 1459, 1573, 1849, 1855, 2616, 2718, 2828 |
| BseYI | CCCAGC | 2 cut(s) | 2881, 2905 |
| BsgI | GTGCAG | 6 cut(s) | 480, 631, 879, 1025, 2598, 2660 |
| Bsh1236I | CGCG | 2 cut(s) | 103, 2602 |
| Bsh1285I | CGRYCG | 3 cut(s) | 2662, 2711, 2845 |
| BshFI | GGCC | 4 cut(s) | 119, 2522, 2645, 2759 |
| BshNI | GGYRCC | 2 cut(s) | 2567, 2664 |
| BshTI | ACCGGT | 1 cut(s) | 2405 |
| BshVI | ATCGAT | 2 cut(s) | 227, 2551 |
| BsiEI | CGRYCG | 3 cut(s) | 2662, 2711, 2845 |
| BsiHKAI | GWGCWC | 1 cut(s) | 237 |
| BsiHKCI | CYCGRG | 1 cut(s) | 569 |
| BsiSI | CCGG | 7 cut(s) | 110, 1711, 2249, 2406, 2565, 2756, 2874 |
| BslFI | GGGAC | 4 cut(s) | 34, 2399, 2698, 2753 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1185, 2279, 2374, 2873 |
| BsmAI | GTCTC | 5 cut(s) | 70, 575, 1018, 1228, 1776 |
| BsmFI | GGGAC | 4 cut(s) | 34, 2399, 2698, 2753 |
| BsmI | GAATGC | 1 cut(s) | 1823 |
| BsnI | GGCC | 4 cut(s) | 119, 2522, 2645, 2759 |
| Bso31I | GGTCTC | 2 cut(s) | 70, 1228 |
| BsoBI | CYCGRG | 1 cut(s) | 569 |
| Bsp119I | TTCGAA | 1 cut(s) | 2174 |
| Bsp1286I | GDGCHC | 2 cut(s) | 237, 2669 |
| Bsp13I | TCCGGA | 1 cut(s) | 1710 |
| Bsp1407I | TGTACA | 1 cut(s) | 1172 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1011 |
| BspACI | CCGC | 8 cut(s) | 107, 153, 2562, 2597, 2600, 2618, 2621, 2753 |
| BspANI | GGCC | 4 cut(s) | 119, 2522, 2645, 2759 |
| BspCNI | CTCAG | 9 cut(s) | 80, 409, 787, 1033, 1297, 1345, 1352, 2013, 2716 |
| BspDI | ATCGAT | 2 cut(s) | 227, 2551 |
| BspEI | TCCGGA | 1 cut(s) | 1710 |
| BspFNI | CGCG | 2 cut(s) | 103, 2602 |
| BspLI | GGNNCC | 4 cut(s) | 2460, 2560, 2569, 2666 |
| BspMAI | CTGCAG | 2 cut(s) | 2736, 2818 |
| BspMI | ACCTGC | 1 cut(s) | 2821 |
| BspPI | GGATC | 5 cut(s) | 45, 1945, 2453, 2466, 2672 |
| BspT104I | TTCGAA | 1 cut(s) | 2174 |
| BspT107I | GGYRCC | 2 cut(s) | 2567, 2664 |
| BspTNI | GGTCTC | 2 cut(s) | 70, 1228 |
| BsrFI | RCCGGY | 3 cut(s) | 2405, 2564, 2755 |
| BsrGI | TGTACA | 1 cut(s) | 1172 |
| BsrI | ACTGG | 3 cut(s) | 979, 2297, 2725 |
| BssAI | RCCGGY | 3 cut(s) | 2405, 2564, 2755 |
| BssECI | CCNNGG | 3 cut(s) | 2504, 2629, 2872 |
| BssNI | GRCGYC | 1 cut(s) | 2594 |
| Bst2UI | CCWGG | 2 cut(s) | 410, 2506 |
| Bst6I | CTCTTC | 1 cut(s) | 103 |
| BstACI | GRCGYC | 1 cut(s) | 2594 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1846 |
| BstAUI | TGTACA | 1 cut(s) | 1172 |
| BstBI | TTCGAA | 1 cut(s) | 2174 |
| BstC8I | GCNNGC | 8 cut(s) | 177, 973, 1017, 1481, 2546, 2566, 2643, 2757 |
| BstDSI | CCRYGG | 1 cut(s) | 2629 |
| BstF5I | GGATG | 7 cut(s) | 237, 418, 754, 1574, 1707, 2760, 2877 |
| BstFNI | CGCG | 2 cut(s) | 103, 2602 |
| BstH2I | RGCGCY | 1 cut(s) | 2888 |
| BstHHI | GCGC | 4 cut(s) | 1873, 2604, 2887, 2911 |
| BstMAI | GTCTC | 5 cut(s) | 70, 575, 1018, 1228, 1776 |
| BstMCI | CGRYCG | 3 cut(s) | 2662, 2711, 2845 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 30, 902, 1016, 1846, 2541 |
| BstNI | CCWGG | 2 cut(s) | 410, 2506 |
| BstNSI | RCATGY | 3 cut(s) | 1454, 1853, 1868 |
| BstSCI | CCNGG | 3 cut(s) | 408, 2504, 2872 |
| BstSFI | CTRYAG | 3 cut(s) | 690, 2732, 2814 |
| BstSLI | GKGCMC | 1 cut(s) | 2669 |
| BstUI | CGCG | 2 cut(s) | 103, 2602 |
| BstV1I | GCAGC | 8 cut(s) | 880, 1459, 1573, 1849, 1855, 2616, 2718, 2828 |
| BstV2I | GAAGAC | 3 cut(s) | 89, 1877, 2251 |
| BstX2I | RGATCY | 5 cut(s) | 193, 1000, 1950, 2458, 2699 |
| BstYI | RGATCY | 5 cut(s) | 193, 1000, 1950, 2458, 2699 |
| Bsu15I | ATCGAT | 2 cut(s) | 227, 2551 |
| BsuI | GTATCC | 1 cut(s) | 1182 |
| BsuRI | GGCC | 4 cut(s) | 119, 2522, 2645, 2759 |
| BsuTUI | ATCGAT | 2 cut(s) | 227, 2551 |
| BtgI | CCRYGG | 1 cut(s) | 2629 |
| BtsCI | GGATG | 7 cut(s) | 237, 418, 754, 1574, 1707, 2760, 2877 |
| BtsI | GCAGTG | 1 cut(s) | 2132 |
| BtsIMutI | CAGTG | 6 cut(s) | 614, 1939, 2132, 2304, 2306, 2634 |
| BveI | ACCTGC | 1 cut(s) | 2821 |
| Cac8I | GCNNGC | 8 cut(s) | 177, 973, 1017, 1481, 2546, 2566, 2643, 2757 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2731 |
| CfoI | GCGC | 4 cut(s) | 1873, 2604, 2887, 2911 |
| Cfr10I | RCCGGY | 3 cut(s) | 2405, 2564, 2755 |
| Cfr13I | GGNCC | 4 cut(s) | 74, 2077, 2521, 2644 |
| ClaI | ATCGAT | 2 cut(s) | 227, 2551 |
| CseI | GACGC | 2 cut(s) | 92, 2602 |
| Csp6I | GTAC | 2 cut(s) | 1173, 2484 |
| CspAI | ACCGGT | 1 cut(s) | 2405 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2108, 2143 |
| CviQI | GTAC | 2 cut(s) | 1173, 2484 |
| DraI | TTTAAA | 2 cut(s) | 292, 1920 |
| DraIII | CACNNNGTG | 1 cut(s) | 2345 |
| EaeI | YGGCCR | 1 cut(s) | 2757 |
| Eam1104I | CTCTTC | 1 cut(s) | 103 |
| EarI | CTCTTC | 1 cut(s) | 103 |
| EciI | GGCGGA | 1 cut(s) | 96 |
| Eco31I | GGTCTC | 2 cut(s) | 70, 1228 |
| Eco47I | GGWCC | 2 cut(s) | 74, 2077 |
| Eco57I | CTGAAG | 7 cut(s) | 1109, 1233, 1445, 1871, 2021, 2048, 2307 |
| Eco88I | CYCGRG | 1 cut(s) | 569 |
| EcoO109I | RGGNCCY | 2 cut(s) | 74, 2644 |
| EcoRI | GAATTC | 2 cut(s) | 2170, 2335 |
| EcoRII | CCWGG | 2 cut(s) | 408, 2504 |
| EcoT22I | ATGCAT | 1 cut(s) | 1851 |
| FaqI | GGGAC | 4 cut(s) | 34, 2399, 2698, 2753 |
| FbaI | TGATCA | 3 cut(s) | 367, 1033, 1690 |
| FokI | GGATG | 7 cut(s) | 224, 425, 761, 1561, 1694, 2747, 2864 |
| GlaI | GCGC | 4 cut(s) | 1872, 2603, 2886, 2910 |
| GsaI | CCCAGC | 2 cut(s) | 2885, 2909 |
| GsuI | CTGGAG | 3 cut(s) | 575, 1757, 2577 |
| HaeII | RGCGCY | 1 cut(s) | 2888 |
| HaeIII | GGCC | 4 cut(s) | 119, 2522, 2645, 2759 |
| HapII | CCGG | 7 cut(s) | 110, 1711, 2249, 2406, 2565, 2756, 2874 |
| HgaI | GACGC | 2 cut(s) | 92, 2602 |
| HhaI | GCGC | 4 cut(s) | 1873, 2604, 2887, 2911 |
| Hin1I | GRCGYC | 1 cut(s) | 2594 |
| Hin6I | GCGC | 4 cut(s) | 1871, 2602, 2885, 2909 |
| HinP1I | GCGC | 4 cut(s) | 1871, 2602, 2885, 2909 |
| HincII | GTYRAC | 2 cut(s) | 1747, 2304 |
| HindII | GTYRAC | 2 cut(s) | 1747, 2304 |
| HindIII | AAGCTT | 5 cut(s) | 130, 177, 1013, 2060, 2319 |
| HpaI | GTTAAC | 1 cut(s) | 1747 |
| HpaII | CCGG | 7 cut(s) | 110, 1711, 2249, 2406, 2565, 2756, 2874 |
| HphI | GGTGA | 7 cut(s) | 142, 728, 1445, 2262, 2331, 2618, 2893 |
| Hpy166II | GTNNAC | 6 cut(s) | 1643, 1747, 1937, 2304, 2486, 2916 |
| Hpy8I | GTNNAC | 6 cut(s) | 1643, 1747, 1937, 2304, 2486, 2916 |
| Hpy99I | CGWCG | 3 cut(s) | 127, 2596, 2893 |
| HpyCH4IV | ACGT | 1 cut(s) | 760 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 30, 902, 1016, 1846, 2541 |
| HpySE526I | ACGT | 1 cut(s) | 760 |
| Hsp92I | GRCGYC | 1 cut(s) | 2594 |
| HspAI | GCGC | 4 cut(s) | 1871, 2602, 2885, 2909 |
| Kpn2I | TCCGGA | 1 cut(s) | 1710 |
| KroI | GCCGGC | 2 cut(s) | 2564, 2755 |
| KroNI | GCCGGC | 2 cut(s) | 2566, 2757 |
| Ksp22I | TGATCA | 3 cut(s) | 367, 1033, 1690 |
| KspAI | GTTAAC | 1 cut(s) | 1747 |
| LmnI | GCTCC | 9 cut(s) | 30, 232, 550, 1507, 2158, 2251, 2282, 2558, 2859 |
| Lsp1109I | GCAGC | 8 cut(s) | 880, 1459, 1573, 1849, 1855, 2616, 2718, 2828 |
| LweI | GCATC | 8 cut(s) | 403, 733, 1066, 1583, 1836, 2557, 2632, 2886 |
| MaeII | ACGT | 1 cut(s) | 760 |
| MfeI | CAATTG | 3 cut(s) | 1085, 2120, 2919 |
| MflI | RGATCY | 5 cut(s) | 193, 1000, 1950, 2458, 2699 |
| MhlI | GDGCHC | 2 cut(s) | 237, 2669 |
| MlyI | GAGTC | 6 cut(s) | 122, 344, 440, 524, 561, 1148 |
| MmeI | TCCRAC | 5 cut(s) | 765, 1179, 1978, 2806, 2852 |
| Mph1103I | ATGCAT | 1 cut(s) | 1851 |
| MroI | TCCGGA | 1 cut(s) | 1710 |
| MroNI | GCCGGC | 2 cut(s) | 2564, 2755 |
| MroXI | GAANNNNTTC | 2 cut(s) | 86, 269 |
| MslI | CAYNNNNRTG | 3 cut(s) | 240, 2046, 2447 |
| MspI | CCGG | 7 cut(s) | 110, 1711, 2249, 2406, 2565, 2756, 2874 |
| MspR9I | CCNGG | 3 cut(s) | 410, 2506, 2874 |
| MunI | CAATTG | 3 cut(s) | 1085, 2120, 2919 |
| Mva1269I | GAATGC | 1 cut(s) | 1823 |
| MvaI | CCWGG | 2 cut(s) | 410, 2506 |
| MvnI | CGCG | 2 cut(s) | 103, 2602 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 30, 902, 1016, 1846, 2541 |
| NaeI | GCCGGC | 2 cut(s) | 2566, 2757 |
| NciI | CCSGG | 1 cut(s) | 2874 |
| NgoMIV | GCCGGC | 2 cut(s) | 2564, 2755 |
| NlaIV | GGNNCC | 4 cut(s) | 2460, 2560, 2569, 2666 |
| NsiI | ATGCAT | 1 cut(s) | 1851 |
| NspI | RCATGY | 3 cut(s) | 1454, 1853, 1868 |
| NspV | TTCGAA | 1 cut(s) | 2174 |
| OliI | CACNNNNGTG | 1 cut(s) | 240 |
| PaeR7I | CTCGAG | 1 cut(s) | 569 |
| PaqCI | CACCTGC | 1 cut(s) | 2821 |
| PctI | GAATGC | 1 cut(s) | 1823 |
| PdiI | GCCGGC | 2 cut(s) | 2566, 2757 |
| PdmI | GAANNNNTTC | 2 cut(s) | 86, 269 |
| PfeI | GAWTC | 9 cut(s) | 538, 641, 1277, 1543, 1567, 1829, 2267, 2805, 2847 |
| PfoI | TCCNGGA | 1 cut(s) | 408 |
| PinAI | ACCGGT | 1 cut(s) | 2405 |
| Ple19I | CGATCG | 2 cut(s) | 2662, 2845 |
| PleI | GAGTC | 6 cut(s) | 121, 343, 439, 523, 561, 1147 |
| PpsI | GAGTC | 6 cut(s) | 121, 343, 439, 523, 561, 1147 |
| PpuMI | RGGWCCY | 1 cut(s) | 74 |
| PshBI | ATTAAT | 1 cut(s) | 1701 |
| Psp5II | RGGWCCY | 1 cut(s) | 74 |
| Psp6I | CCWGG | 2 cut(s) | 408, 2504 |
| PspFI | CCCAGC | 2 cut(s) | 2881, 2905 |
| PspGI | CCWGG | 2 cut(s) | 408, 2504 |
| PspN4I | GGNNCC | 4 cut(s) | 2460, 2560, 2569, 2666 |
| PspPI | GGNCC | 4 cut(s) | 74, 2077, 2521, 2644 |
| PspPPI | RGGWCCY | 1 cut(s) | 74 |
| PstI | CTGCAG | 2 cut(s) | 2736, 2818 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2731 |
| PsuI | RGATCY | 5 cut(s) | 193, 1000, 1950, 2458, 2699 |
| PvuI | CGATCG | 2 cut(s) | 2662, 2845 |
| RsaI | GTAC | 2 cut(s) | 1174, 2485 |
| RsaNI | GTAC | 2 cut(s) | 1173, 2484 |
| RseI | CAYNNNNRTG | 3 cut(s) | 240, 2046, 2447 |
| Sau96I | GGNCC | 4 cut(s) | 74, 2077, 2521, 2644 |
| SchI | GAGTC | 6 cut(s) | 122, 344, 440, 524, 561, 1148 |
| ScrFI | CCNGG | 3 cut(s) | 410, 2506, 2874 |
| SduI | GDGCHC | 2 cut(s) | 237, 2669 |
| SfaNI | GCATC | 8 cut(s) | 403, 733, 1066, 1583, 1836, 2557, 2632, 2886 |
| SfcI | CTRYAG | 3 cut(s) | 690, 2732, 2814 |
| Sfr274I | CTCGAG | 1 cut(s) | 569 |
| SfuI | TTCGAA | 1 cut(s) | 2174 |
| SinI | GGWCC | 2 cut(s) | 74, 2077 |
| SlaI | CTCGAG | 1 cut(s) | 569 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 240, 2046, 2447 |
| SmlI | CTYRAG | 4 cut(s) | 569, 728, 854, 1634 |
| SmoI | CTYRAG | 4 cut(s) | 569, 728, 854, 1634 |
| SsiI | CCGC | 8 cut(s) | 107, 153, 2562, 2597, 2600, 2618, 2621, 2753 |
| SspI | AATATT | 1 cut(s) | 2356 |
| StyD4I | CCNGG | 3 cut(s) | 408, 2504, 2872 |
| TaiI | ACGT | 1 cut(s) | 763 |
| TaqI | TCGA | 9 cut(s) | 56, 227, 570, 945, 1780, 2174, 2551, 2696, 2845 |
| TaqII | GACCGA | 1 cut(s) | 52 |
| TatI | WGTACW | 2 cut(s) | 1172, 2483 |
| TauI | GCSGC | 5 cut(s) | 2564, 2599, 2602, 2623, 2755 |
| TfiI | GAWTC | 9 cut(s) | 538, 641, 1277, 1543, 1567, 1829, 2267, 2805, 2847 |
| TscAI | CASTG | 6 cut(s) | 614, 1939, 2132, 2304, 2313, 2641 |
| TseI | GCWGC | 8 cut(s) | 893, 1447, 1586, 1837, 1868, 2604, 2731, 2816 |
| TspGWI | ACGGA | 3 cut(s) | 230, 2753, 2817 |
| TspRI | CASTG | 6 cut(s) | 614, 1939, 2132, 2304, 2313, 2641 |
| VpaK11BI | GGWCC | 2 cut(s) | 74, 2077 |
| VspI | ATTAAT | 1 cut(s) | 1701 |
| XapI | RAATTY | 4 cut(s) | 1131, 1915, 2170, 2335 |
| XbaI | TCTAGA | 1 cut(s) | 934 |
| XceI | RCATGY | 3 cut(s) | 1454, 1853, 1868 |
| XhoI | CTCGAG | 1 cut(s) | 569 |
| XmnI | GAANNNNTTC | 2 cut(s) | 86, 269 |
| Zsp2I | ATGCAT | 1 cut(s) | 1851 |