MD08G1202600.v1.1

Sas10/Utp3/C1D family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008750
Physical Location & Seq
Forward (+)
26211294 .. 26214533
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1202600.v1.1.491

Sequence Viewer

Length: 897 bp
ATGAAGGAAGGATTAGATACAGTGAGGCCAAAAGTCCAAGCTTTAACTGCAAAGGTGAAAGCAAATAATTATCCTACAGCAGATGGGATAAGCTATCTTGAAGCCAAGCATTTGCTGCTTCTAAACTATTGCCAGTCACTTGTCTATTACTTACTTCGTAAGGCTAAAGGGTTTTCAATAAATGGGCATCCCGTTGTTCGTAGCCTTGTGGAGATAAGGTTGTTTCTGGAAAAGATTCGACCCATTGACAAGAAGCTCCAGTACCAAGTTGAGAAGCTCACCAAGGTTACTACAAGCACAACCGAAGATGTAGGGCAAAGTGGGAAGGAACCACAACCCAATGTGCCTCAGCAAACAGATGATTTGCTGAGATACCGCCCTAACCCTGACTTGCTTGTTAGCAAAACAGATGTGAATTTTAAGGATGGCAAGGATGTGTATCGACCTCCCAAATTTGCCCCGACTTCGATGGAGGAAGATAAGTTGTCAAAGCAGGAAAAAAATGCGTTGAGAAGGGATAAGAATACTTTACGACAAGCTAAGCAGAGTGCTTATGTGAGGGAACTGGTGGATGATTTGGAGGGGAGAGGCCTGAAGAGGTTTATTGTAGAAAGTGTTGGAGCTGAAAGCATAGAACTAGCGAGATACAGGGCAAAGAGGGATGAACGTGAACGGCAAGAAGAGGAACTTTTTACGCGTGCTCCTATTACAAAGAAGGAGAAACAGAGAGAAAAACACTTAAAGAAATCAAGAAATGGGTTGCTTGGTTTGACGGAGAATTTCTATGATGAAATTAAGGGTTTACCCTTGGACGATGAGAATGATGAGCAGGTTGCAAGCTCCCGCAGTGCTAGGGGTGGAATGGGGAGGCATAAGAAGCGCAAGATGAGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sas10_Utp3

Protein Analysis

299

Amino Acids

34.41

Weight (kDa)

9.7

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sas10_Utp3 PF04000 1 - 78 7.8e-19 Sas10/Utp3/C1D family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 820
AccII CGCG 1 cut(s) 697
AciI CCGC 2 cut(s) 376, 844
AcsI RAATTY 3 cut(s) 415, 452, 778
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 1 cut(s) 263
AflIII ACRYGT 1 cut(s) 695
AgsI TTSAA 2 cut(s) 101, 177
AluBI AGCT 7 cut(s) 41, 93, 256, 277, 539, 623, 840
AluI AGCT 7 cut(s) 41, 93, 256, 277, 539, 623, 840
Alw21I GWGCWC 1 cut(s) 703
AoxI GGCC 2 cut(s) 26, 589
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 3 cut(s) 415, 452, 778
Asp700I GAANNNNTTC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 882
AsuHPI GGTGA 2 cut(s) 67, 271
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
Bbv12I GWGCWC 1 cut(s) 703
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 102
BccI CCATC 3 cut(s) 77, 419, 463
BceAI ACGGC 1 cut(s) 689
BfaI CTAG 2 cut(s) 638, 852
BfmI CTRYAG 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 820
BisI GCNGC 1 cut(s) 116
BlpI GCTNAGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 117
BmiI GGNNCC 1 cut(s) 330
BmsI GCATC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 540
BsaBI GATNNNNATC 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 282, 807
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 685, 715
Bse1I ACTGG 3 cut(s) 133, 259, 570
Bse8I GATNNNNATC 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 282, 807
BseGI GGATG 5 cut(s) 187, 430, 439, 577, 667
BseJI GATNNNNATC 1 cut(s) 438
BseMII CTCAG 2 cut(s) 359, 362
BseNI ACTGG 3 cut(s) 133, 259, 570
BseXI GCAGC 1 cut(s) 102
Bsh1236I CGCG 1 cut(s) 697
BshFI GGCC 2 cut(s) 28, 591
BsiHKAI GWGCWC 1 cut(s) 703
BsnI GGCC 2 cut(s) 28, 591
Bsp1286I GDGCHC 1 cut(s) 703
Bsp1720I GCTNAGC 1 cut(s) 540
BspACI CCGC 2 cut(s) 376, 844
BspANI GGCC 2 cut(s) 28, 591
BspCNI CTCAG 2 cut(s) 360, 361
BspFNI CGCG 1 cut(s) 697
BspLI GGNNCC 1 cut(s) 330
BspMI ACCTGC 1 cut(s) 820
BsrI ACTGG 3 cut(s) 133, 259, 570
BssECI CCNNGG 2 cut(s) 282, 807
BssT1I CCWWGG 2 cut(s) 282, 807
Bst4CI ACNGT 1 cut(s) 22
Bst6I CTCTTC 2 cut(s) 590, 675
BstAPI GCANNNNNTGC 1 cut(s) 115
BstC8I GCNNGC 2 cut(s) 699, 838
BstDEI CTNAG 3 cut(s) 348, 368, 540
BstF5I GGATG 5 cut(s) 187, 430, 439, 577, 667
BstFNI CGCG 1 cut(s) 697
BstHHI GCGC 1 cut(s) 882
BstMWI GCNNNNNNNGC 3 cut(s) 47, 115, 877
BstSFI CTRYAG 1 cut(s) 75
BstUI CGCG 1 cut(s) 697
BstV1I GCAGC 1 cut(s) 102
BsuRI GGCC 2 cut(s) 28, 591
BtsCI GGATG 5 cut(s) 187, 430, 439, 577, 667
BtsI GCAGTG 1 cut(s) 853
BtsIMutI CAGTG 2 cut(s) 27, 853
BveI ACCTGC 1 cut(s) 820
Cac8I GCNNGC 2 cut(s) 699, 838
CfoI GCGC 1 cut(s) 882
Csp6I GTAC 1 cut(s) 262
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 3 cut(s) 348, 368, 540
Eam1104I CTCTTC 2 cut(s) 590, 675
EarI CTCTTC 2 cut(s) 590, 675
Eco130I CCWWGG 2 cut(s) 282, 807
Eco147I AGGCCT 1 cut(s) 591
Eco57I CTGAAG 1 cut(s) 614
EcoT14I CCWWGG 2 cut(s) 282, 807
ErhI CCWWGG 2 cut(s) 282, 807
FaiI YATR 4 cut(s) 555, 632, 786, 873
FalI AAGNNNNNCTT 2 cut(s) 672, 704
FauI CCCGC 1 cut(s) 851
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 5 cut(s) 174, 437, 446, 584, 674
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 2 cut(s) 638, 852
GlaI GCGC 1 cut(s) 881
GluI GCNGC 1 cut(s) 116
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 2 cut(s) 28, 591
HhaI GCGC 1 cut(s) 882
Hin6I GCGC 1 cut(s) 880
HinP1I GCGC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 1 cut(s) 235
HphI GGTGA 2 cut(s) 67, 271
Hpy166II GTNNAC 2 cut(s) 671, 803
Hpy188III TCNNGA 3 cut(s) 98, 227, 750
Hpy8I GTNNAC 2 cut(s) 671, 803
HpyAV CCTTC 3 cut(s) 319, 507, 709
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 1 cut(s) 667
HpyCH4V TGCA 2 cut(s) 50, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 115, 877
HpyF3I CTNAG 3 cut(s) 348, 368, 540
HpySE526I ACGT 1 cut(s) 667
HspAI GCGC 1 cut(s) 880
LmnI GCTCC 4 cut(s) 261, 620, 706, 845
LpnPI CCDG 9 cut(s) 146, 212, 272, 399, 479, 551, 605, 634, 815
Lsp1109I GCAGC 1 cut(s) 102
LweI GCATC 1 cut(s) 196
MaeI CTAG 2 cut(s) 638, 852
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 2 cut(s) 135, 286
MboII GAAGA 4 cut(s) 317, 488, 607, 692
MhlI GDGCHC 1 cut(s) 703
MluCI AATT 5 cut(s) 67, 415, 452, 778, 792
MluI ACGCGT 1 cut(s) 695
MmeI TCCRAC 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 4 cut(s) 44, 420, 740, 795
MvnI CGCG 1 cut(s) 697
MwoI GCNNNNNNNGC 3 cut(s) 47, 115, 877
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 135
PceI AGGCCT 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 330
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SaqAI TTAA 4 cut(s) 44, 420, 740, 795
SatI GCNGC 1 cut(s) 116
SduI GDGCHC 1 cut(s) 703
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 1 cut(s) 75
Sse9I AATT 5 cut(s) 67, 415, 452, 778, 792
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 2 cut(s) 376, 844
SspMI CTAG 2 cut(s) 638, 852
StuI AGGCCT 1 cut(s) 591
StyI CCWWGG 2 cut(s) 282, 807
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 1 cut(s) 670
TaqI TCGA 3 cut(s) 238, 442, 467
TasI AATT 5 cut(s) 67, 415, 452, 778, 792
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 4 cut(s) 44, 420, 740, 795
Tru9I TTAA 4 cut(s) 44, 420, 740, 795
TscAI CASTG 2 cut(s) 27, 853
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 3 cut(s) 17, 678, 804
TspGWI ACGGA 1 cut(s) 788
TspRI CASTG 2 cut(s) 27, 853
XapI RAATTY 3 cut(s) 415, 452, 778
XmnI GAANNNNTTC 1 cut(s) 234
XspI CTAG 2 cut(s) 638, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.