MD08G1236900.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006641
Physical Location & Seq
Reverse (-)
30435157 .. 30441656
6500 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1236900.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGAGGAGATGATACAGCAACAGAGGTCGTTGGCCGAGACGCCAACGTATTCAGTTGCTTCGGTTATTATTGTTATGGTCTTTGTCTGCCTTCTGGTAGAAAGGTCAATCTACAGATGTGGAAAGTGGTTGAAGAAGACAAGGAGGAAAGCGCTGTTTGCTTCTTTGGAGAAGATTAAGGAAGAATTGATGCTGCTTGGCCTAATATCGTTGTTGTTGTCACAATCGGCAAGGTGGATCTCTGGGATTTGTGTAAACTCTTCCCTTTTCACCAGTCAATTTTACATCTGTTCCGAGGAAGATGACAGCACCAAGAAGCATGTCATGCTTGAAAGTTCTCCTAACAAGACTGAGATTCCAAGAGGATTAACTAATTTTTCTCATCAATGTGGTGAGGGCCGGGAACCGTTTGTTTCTTATGAGGGACTGGAGCAGCTGCACCGGTTCCTGTTCGTTCTTGGGATCACCCATGTGTTGTACAGTTGTTTGGCTGTGGCTTTGGCTATGAGCAAGATTTACAGTTGGAGGAAATGGGAAAATCAAGCGATCTCATTGGCTGATGCGAATTTTCAAGCAAAGAAAAACAAGGTCATGAGGCGGCAGTCTACCTTTATTTTCCATCACACATCTCATCCATGGAGCAGGAATCGGCTTCTAATTTGGATGCTCTGCTTTCTACGACAATTTAAGAGTTCTATAGAGAGATCAGATTACTTGGCTCTGCGTTCGGGCTTCATCAGTAAGCACAAACTTCCGCTTTCTTATAATTTTCATAAATACATGGTCCGGAGTATGGAAGATGAATTTCAGGGCATTCTGGGTATTAGCTGCCCACTTTGGGGATATTCCATAGTCTGCATATTCGTAAATGTTCATGGTTTGAATATTTACTTTTGGCTTTCTTTCATCCCTGCCATTCTTGTCATGCTGGTTGGAACGAAACTACAGCATGTCGTCTCTTCCTTAGCACTTGAAGTGGAACAAACAGGGCCAGCTAATGGGACTCAAGTAAAGCCACGTGATGATTTGTTTTGGTTTGGAAAACCTGTGATACTATTGCGGTTGATACAGTTCATCATATTTCAGAATGCCTTTGAGATGGCGACATTTCTCTGGTCCTTGTGGGGGCTCAAGGAGAGATCATGCTTCATGAAAAACCACTTAATGATCGCTATTCGGTTGACCTGTGGTGTTCTTGTTCAGTTCTGGTGTAGTTACGGCACAGTGCCTCTGAACGTAATTGTTACACAGATGGGATCCCAGTGTAAGAAAGCTCTTGTTGCTGAGAGTGTTAGAGATTCGCTCCATAGTTGGTGCAAGCGAGTAAAGGAGAGGTCTAAACAATCACACGCAACAAGGTCAGTTTGTTCACTTGATACAACAGACGAGAGGGATGAGATAACCGTTGCATCTGGTACCTTGTCTCGGAACTCATCCTTGGACTCCCTACATCAAGTAACGGTAGCACCAATGGAACAGCAACCCGAGGCTGATCTACAGACTTCCGGACCACCTCAGCATGACTACTCATTTCGAGTGGAAGAGTATTTATCTGATGCGGTGAGAATGAGTGCATCACAGCCTCCGATAAACAGCGATGAAGATGATGGAGTGGAAGAACAAATGGATACTCTATTAGAGTTGTTTCAGAAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

553

Amino Acids

63.28

Weight (kDa)

8.4

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 462 1e-171 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 765
Acc65I GGTACC 1 cut(s) 1415
AccB1I GGYRCC 1 cut(s) 1415
AccB7I CCANNNNNTGG 1 cut(s) 998
AccI GTMKAC 1 cut(s) 605
AccIII TCCGGA 2 cut(s) 786, 1505
AciI CCGC 4 cut(s) 598, 755, 1060, 1559
AclWI GGATC 4 cut(s) 245, 470, 1251, 1264
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 2 cut(s) 565, 803
AcvI CACGTG 1 cut(s) 1019
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 2 cut(s) 479, 1417
AfeI AGCGCT 1 cut(s) 153
AfiI CCNNNNNNNGG 6 cut(s) 793, 838, 839, 998, 1125, 1425
AgeI ACCGGT 1 cut(s) 441
AgsI TTSAA 5 cut(s) 133, 332, 572, 883, 974
AjuI GAANNNNNNNTTGG 2 cut(s) 1421, 1453
AleI CACNNNNGTG 1 cut(s) 470
AluBI AGCT 4 cut(s) 436, 828, 995, 1274
AluI AGCT 4 cut(s) 436, 828, 995, 1274
Alw26I GTCTC 3 cut(s) 32, 961, 1428
AlwI GGATC 4 cut(s) 245, 470, 1251, 1264
Ama87I CYCGRG 1 cut(s) 1484
Aor13HI TCCGGA 2 cut(s) 786, 1505
Aor51HI AGCGCT 1 cut(s) 153
AoxI GGCC 4 cut(s) 33, 199, 397, 989
ApeKI GCWGC 4 cut(s) 193, 433, 436, 828
ApoI RAATTY 2 cut(s) 565, 803
AsiGI ACCGGT 1 cut(s) 441
Asp718I GGTACC 1 cut(s) 1415
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 5 cut(s) 397, 784, 989, 1116, 1508
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 4 cut(s) 262, 404, 457, 1573
AvaI CYCGRG 1 cut(s) 1484
AvaII GGWCC 3 cut(s) 784, 1116, 1508
BamHI GGATCC 1 cut(s) 1256
BanI GGYRCC 1 cut(s) 1415
BanII GRGCYC 1 cut(s) 1131
BbrPI CACGTG 1 cut(s) 1019
BbsI GAAGAC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 1515
BbvI GCAGC 4 cut(s) 180, 423, 445, 815
BccI CCATC 4 cut(s) 627, 1093, 1246, 1601
BceAI ACGGC 1 cut(s) 1234
BciVI GTATCC 1 cut(s) 1621
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 3 cut(s) 32, 961, 1428
BfmI CTRYAG 4 cut(s) 112, 696, 944, 1496
BfoI RGCGCY 1 cut(s) 155
BfuI GTATCC 1 cut(s) 1621
BisI GCNGC 5 cut(s) 194, 434, 437, 599, 829
BlsI GCNGC 5 cut(s) 195, 435, 438, 600, 830
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 3 cut(s) 784, 1116, 1508
BmeT110I CYCGRG 1 cut(s) 1484
BmgT120I GGNCC 5 cut(s) 397, 784, 989, 1116, 1508
BmiI GGNNCC 4 cut(s) 405, 446, 1258, 1417
BmrFI CCNGG 1 cut(s) 401
BmrI ACTGGG 1 cut(s) 1255
BmsI GCATC 6 cut(s) 180, 550, 654, 1418, 1546, 1583
BmuI ACTGGG 1 cut(s) 1255
BpiI GAAGAC 1 cut(s) 143
BplI GAGNNNNNCTC 2 cut(s) 1287, 1319
BpmI CTGGAG 1 cut(s) 449
Bpu10I CCTNAGC 2 cut(s) 964, 1515
BpuEI CTTGAG 2 cut(s) 990, 1115
BpuMI CCSGG 1 cut(s) 401
BsaAI YACGTR 1 cut(s) 1019
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 4 cut(s) 294, 635, 1437, 1485
BsaWI WCCGGW 3 cut(s) 441, 786, 1505
Bsc4I CCNNNNNNNGG 6 cut(s) 793, 838, 839, 998, 1125, 1425
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 3 cut(s) 273, 432, 1261
BseAI TCCGGA 2 cut(s) 786, 1505
BseDI CCNNGG 4 cut(s) 294, 635, 1437, 1485
BseGI GGATG 5 cut(s) 631, 669, 906, 1399, 1433
BseLI CCNNNNNNNGG 6 cut(s) 793, 838, 839, 998, 1125, 1425
BseMII CTCAG 3 cut(s) 342, 1275, 1529
BseNI ACTGG 3 cut(s) 273, 432, 1261
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 180, 423, 445, 815
BsgI GTGCAG 1 cut(s) 422
BshFI GGCC 4 cut(s) 35, 201, 399, 991
BshNI GGYRCC 1 cut(s) 1415
BshTI ACCGGT 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 1484
BsiSI CCGG 4 cut(s) 400, 442, 787, 1506
BslFI GGGAC 2 cut(s) 438, 1015
BslI CCNNNNNNNGG 6 cut(s) 793, 838, 839, 998, 1125, 1425
BsmAI GTCTC 3 cut(s) 32, 961, 1428
BsmBI CGTCTC 2 cut(s) 32, 961
BsmFI GGGAC 2 cut(s) 438, 1015
BsmI GAATGC 2 cut(s) 813, 1093
BsnI GGCC 4 cut(s) 35, 201, 399, 991
BsoBI CYCGRG 1 cut(s) 1484
Bsp1286I GDGCHC 1 cut(s) 1131
Bsp13I TCCGGA 2 cut(s) 786, 1505
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
Bsp19I CCATGG 1 cut(s) 635
BspACI CCGC 4 cut(s) 598, 755, 1060, 1559
BspANI GGCC 4 cut(s) 35, 201, 399, 991
BspCNI CTCAG 3 cut(s) 343, 1276, 1528
BspEI TCCGGA 2 cut(s) 786, 1505
BspHI TCATGA 2 cut(s) 591, 1149
BspLI GGNNCC 4 cut(s) 405, 446, 1258, 1417
BspPI GGATC 4 cut(s) 245, 470, 1251, 1264
BspT107I GGYRCC 1 cut(s) 1415
BsrFI RCCGGY 1 cut(s) 441
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 3 cut(s) 273, 432, 1261
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 4 cut(s) 294, 635, 1437, 1485
BssMI GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 2 cut(s) 635, 1437
Bst4CI ACNGT 7 cut(s) 408, 482, 521, 1071, 1225, 1405, 1462
Bst6I CTCTTC 3 cut(s) 265, 964, 1536
BstACI GRCGYC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 325
BstAUI TGTACA 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 2 cut(s) 993, 1319
BstDEI CTNAG 4 cut(s) 351, 964, 1284, 1515
BstDSI CCRYGG 1 cut(s) 635
BstF5I GGATG 5 cut(s) 631, 669, 906, 1399, 1433
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 8 cut(s) 240, 465, 549, 707, 1142, 1170, 1259, 1495
BstMAI GTCTC 3 cut(s) 32, 961, 1428
BstMBI GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
BstMWI GCNNNNNNNGC 3 cut(s) 158, 325, 1280
BstNSI RCATGY 2 cut(s) 323, 953
BstSCI CCNGG 1 cut(s) 399
BstSFI CTRYAG 4 cut(s) 112, 696, 944, 1496
BstV1I GCAGC 4 cut(s) 180, 423, 445, 815
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 237, 1256
BstYI RGATCY 2 cut(s) 237, 1256
BsuI GTATCC 1 cut(s) 1621
BsuRI GGCC 4 cut(s) 35, 201, 399, 991
BtgI CCRYGG 1 cut(s) 635
BtgZI GCGATG 1 cut(s) 1611
BtsCI GGATG 5 cut(s) 631, 669, 906, 1399, 1433
BtsIMutI CAGTG 2 cut(s) 1230, 1268
Cac8I GCNNGC 2 cut(s) 993, 1319
CciI TCATGA 2 cut(s) 591, 1149
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 441
Cfr13I GGNCC 5 cut(s) 397, 784, 989, 1116, 1508
CseI GACGC 1 cut(s) 49
Csp6I GTAC 2 cut(s) 478, 1416
CspAI ACCGGT 1 cut(s) 441
CviQI GTAC 2 cut(s) 478, 1416
DdeI CTNAG 4 cut(s) 351, 964, 1284, 1515
DpnI GATC 8 cut(s) 239, 464, 548, 706, 1141, 1169, 1258, 1494
DpnII GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 3 cut(s) 265, 964, 1536
EarI CTCTTC 3 cut(s) 265, 964, 1536
Eco130I CCWWGG 2 cut(s) 635, 1437
Eco24I GRGCYC 1 cut(s) 1131
Eco47I GGWCC 3 cut(s) 784, 1116, 1508
Eco47III AGCGCT 1 cut(s) 153
Eco72I CACGTG 1 cut(s) 1019
Eco88I CYCGRG 1 cut(s) 1484
EcoT14I CCWWGG 2 cut(s) 635, 1437
EcoT38I GRGCYC 1 cut(s) 1131
ErhI CCWWGG 2 cut(s) 635, 1437
Esp3I CGTCTC 2 cut(s) 32, 961
FaqI GGGAC 2 cut(s) 438, 1015
FblI GTMKAC 1 cut(s) 605
Fnu4HI GCNGC 5 cut(s) 194, 434, 437, 599, 829
FokI GGATG 5 cut(s) 618, 676, 893, 1406, 1420
FriOI GRGCYC 1 cut(s) 1131
Fsp4HI GCNGC 5 cut(s) 194, 434, 437, 599, 829
GlaI GCGC 1 cut(s) 153
GluI GCNGC 5 cut(s) 194, 434, 437, 599, 829
GsuI CTGGAG 1 cut(s) 449
HaeII RGCGCY 1 cut(s) 155
HaeIII GGCC 4 cut(s) 35, 201, 399, 991
HapII CCGG 4 cut(s) 400, 442, 787, 1506
HgaI GACGC 1 cut(s) 49
HhaI GCGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 41
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 1 cut(s) 1182
HindII GTYRAC 1 cut(s) 1182
HinfI GANTC 5 cut(s) 355, 646, 1003, 1298, 1442
HpaII CCGG 4 cut(s) 400, 442, 787, 1506
HphI GGTGA 4 cut(s) 262, 404, 457, 1573
Hpy166II GTNNAC 4 cut(s) 256, 606, 1182, 1370
Hpy188I TCNGA 8 cut(s) 295, 709, 1086, 1233, 1428, 1555, 1587, 1650
Hpy188III TCNNGA 4 cut(s) 592, 787, 1150, 1506
Hpy8I GTNNAC 4 cut(s) 256, 606, 1182, 1370
HpyAV CCTTC 1 cut(s) 101
HpyCH4III ACNGT 7 cut(s) 408, 482, 521, 1071, 1225, 1405, 1462
HpyCH4IV ACGT 3 cut(s) 47, 1018, 1236
HpyCH4V TGCA 5 cut(s) 439, 858, 1317, 1409, 1574
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 325, 1280
HpyF3I CTNAG 4 cut(s) 351, 964, 1284, 1515
HpySE526I ACGT 3 cut(s) 47, 1018, 1236
Hsp92I GRCGYC 1 cut(s) 41
HspAI GCGC 1 cut(s) 152
Kpn2I TCCGGA 2 cut(s) 786, 1505
KpnI GGTACC 1 cut(s) 1419
Kzo9I GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
LmnI GCTCC 3 cut(s) 430, 639, 1308
Lsp1109I GCAGC 4 cut(s) 180, 423, 445, 815
LweI GCATC 6 cut(s) 180, 550, 654, 1418, 1546, 1583
MaeII ACGT 3 cut(s) 47, 1018, 1236
MaeIII GTNAC 4 cut(s) 219, 1214, 1243, 1456
MalI GATC 8 cut(s) 239, 464, 548, 706, 1141, 1169, 1258, 1494
MboI GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
MflI RGATCY 2 cut(s) 237, 1256
MhlI GDGCHC 1 cut(s) 1131
MluCI AATT 9 cut(s) 185, 278, 373, 565, 657, 683, 766, 803, 1239
MlyI GAGTC 2 cut(s) 997, 1436
MmeI TCCRAC 2 cut(s) 503, 913
MroI TCCGGA 2 cut(s) 786, 1505
MseI TTAA 4 cut(s) 177, 368, 687, 1163
MslI CAYNNNNRTG 2 cut(s) 387, 470
MspA1I CMGCKG 1 cut(s) 436
MspI CCGG 4 cut(s) 400, 442, 787, 1506
MspR9I CCNGG 1 cut(s) 401
Mva1269I GAATGC 2 cut(s) 813, 1093
MwoI GCNNNNNNNGC 3 cut(s) 158, 325, 1280
NciI CCSGG 1 cut(s) 401
NcoI CCATGG 1 cut(s) 635
NdeII GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
NlaIV GGNNCC 4 cut(s) 405, 446, 1258, 1417
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 219
NspI RCATGY 2 cut(s) 323, 953
OliI CACNNNNGTG 1 cut(s) 470
PagI TCATGA 2 cut(s) 591, 1149
PctI GAATGC 2 cut(s) 813, 1093
PfeI GAWTC 3 cut(s) 355, 646, 1298
PflMI CCANNNNNTGG 1 cut(s) 998
PinAI ACCGGT 1 cut(s) 441
PkrI GCNGC 5 cut(s) 195, 435, 438, 600, 830
PleI GAGTC 2 cut(s) 997, 1436
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
PpsI GAGTC 2 cut(s) 997, 1436
Ppu21I YACGTR 1 cut(s) 1019
PsiI TTATAA 1 cut(s) 765
PspCI CACGTG 1 cut(s) 1019
PspN4I GGNNCC 4 cut(s) 405, 446, 1258, 1417
PspPI GGNCC 5 cut(s) 397, 784, 989, 1116, 1508
PsuI RGATCY 2 cut(s) 237, 1256
PvuII CAGCTG 1 cut(s) 436
RsaI GTAC 2 cut(s) 479, 1417
RsaNI GTAC 2 cut(s) 478, 1416
RseI CAYNNNNRTG 2 cut(s) 387, 470
SaqAI TTAA 4 cut(s) 177, 368, 687, 1163
SatI GCNGC 5 cut(s) 194, 434, 437, 599, 829
Sau3AI GATC 8 cut(s) 237, 462, 546, 704, 1139, 1167, 1256, 1492
Sau96I GGNCC 5 cut(s) 397, 784, 989, 1116, 1508
SchI GAGTC 2 cut(s) 997, 1436
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 1 cut(s) 1131
SfaNI GCATC 6 cut(s) 180, 550, 654, 1418, 1546, 1583
SfcI CTRYAG 4 cut(s) 112, 696, 944, 1496
SinI GGWCC 3 cut(s) 784, 1116, 1508
SmiMI CAYNNNNRTG 2 cut(s) 387, 470
SmlI CTYRAG 2 cut(s) 1005, 1130
SmoI CTYRAG 2 cut(s) 1005, 1130
Sse9I AATT 9 cut(s) 185, 278, 373, 565, 657, 683, 766, 803, 1239
SsiI CCGC 4 cut(s) 598, 755, 1060, 1559
SspI AATATT 1 cut(s) 886
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 2 cut(s) 635, 1437
TaaI ACNGT 7 cut(s) 408, 482, 521, 1071, 1225, 1405, 1462
TaiI ACGT 3 cut(s) 50, 1021, 1239
TaqI TCGA 1 cut(s) 1534
TasI AATT 9 cut(s) 185, 278, 373, 565, 657, 683, 766, 803, 1239
TatI WGTACW 1 cut(s) 477
TauI GCSGC 1 cut(s) 601
TfiI GAWTC 3 cut(s) 355, 646, 1298
Tru1I TTAA 4 cut(s) 177, 368, 687, 1163
Tru9I TTAA 4 cut(s) 177, 368, 687, 1163
TscAI CASTG 2 cut(s) 1230, 1268
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 4 cut(s) 193, 433, 436, 828
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 9 cut(s) 724, 761, 816, 863, 895, 1063, 1138, 1166, 1614
TspRI CASTG 2 cut(s) 1230, 1268
Van91I CCANNNNNTGG 1 cut(s) 998
VpaK11BI GGWCC 3 cut(s) 784, 1116, 1508
XapI RAATTY 2 cut(s) 565, 803
XceI RCATGY 2 cut(s) 323, 953
XmiI GTMKAC 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.