Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006107

Physical Location & Seq

Genomic Coordinates Forward (+)

31212218 .. 31222006

Gene Structure 9789 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1248100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGATAGTAAGAACTCCCCCGACGAAGAAGCAACGGGCGGCGGCGCCGGAGGCGGAGACCCCACCGCCTGCCCCAGGCCCTGGCCCTCTCGTCATCTTCGAGGACGACCCTCCTCCTCCTTCTTCTTCCCTTCCACCTCCCGATTCCTCCTCCCACCACTTGCTCTGCACCTATCAATGCCGCCAAATGGTTAAATCAGACTTTATAGACGCCTTGAGCAACACGGAGAAGCAAGCTTCTGATTATCAATCTAAATTGGCAGCTTTAAATGAGAAATTTTGCAAAGTTGAATCTGAGAGGAAGAAATATCTGGATCAATTTTTGTATGTGGAACAAGAACTTGCTGCTGCAAAGGGACGTGAACAGGCTCTGCAGGAGCAGCTTTTGAAGGAGTTACACGATTCTCATGAAAGATTGACTAAACAATTACAATCAAACAGCGAACTTGAGGTGAAGCTTCAAAATGAAATGAACCTTCGCATGAAAGCTGAGTCTTCAGCAGCTTTGGCTGAGGAGAAAGCAAGTGATGTAGAGGGGAAACTGAGACATCTTTCTGAAAACATAGAGAGGGAAAAAAAGCGTCTCAATAACGATTTTTCCCAGCTGAAAGGAGAATCCAAGCTTTCTGTTTCTAGAATAACAGCAGATCTTGAACGAATGGAATGCAGATCTCATAATGCTGAGAAAGAATCAGAGCTATTGAAAAAGCAGCTGGATGATATTAAGAAACAACTTAGCGAGTGCGTGCAACAAAAAAGCGAAGTGGAGAAGAAACTGTCAAGTTTTACATTTCAAGAAGTTGAATCCACGGAGAATAATATTCTTGTTAAACATCTGCAAGAAGAGCTTAGAAACTTTGATGCTGAAGTGAGGGAAGCAAGAAAGCTGAAGTTGGCTCATGAAAATGTTGAGTTATTGAAGGAAAAATTGTTGGAAGAAAAGGGCCGCAGAGAGAGGGCAGAGGCAGAGTTATCCAAACTACAAGAATTACAACTTAGTATGAAGAAGTTGGAGAATGAATTGACAACTTGGAAGTTGATGAGTAAAGACATTCCAGGCGTGTCATGTTCTGAGGATATACCTGTTAAATTTGCAGCTTTACAAAAAGAGGTAATTGATAGCATGATGAAAGTGGGTGAGGCTAATGCTGCCTTGAAACAACTGGAGGTAGCTCTAGATGCTGCAAAAATTGATAAACAAAATGCTGAAATAGAGGCTAACCTGGCGAAAGAGAAGGTGGATGTATCAAAATCAGAGGTTAAGCGGATTGAATTAATGCTCTCTATGGTTACTGAGGAAAGGGATAAGTTGAGAAATGTCCTTAATGGACTGAAGTTGGTAAAGAATGATGAAGCTGGAGATGAAGCAGCCAGTCAAACTTTTCTTCAGGAGGTTGAATCATCACTTGCAAAGAAAGAGGCCTATATTAAAGAATTGGAATGTGGTCTGTGTGAACAAAAAGAAGTTAATAGTCGTCAACGTGAAGAAATAAAGTTGCTTAATGAGAGGTTGAACAATGAAGCTAGAAGAATGAAGTCATTGGAGAGGGAGAGCGATCGACTTCGTTCAGAGATCGCTCTGCTGGAGTCCAAGATTGGTCATGGCGATTTCTCTGCTGCAAATACTAAAGTTTTAAGAATGGTGAATACTCTTGCGGCTGATAATGAAGCAAAACAAACTATTGAGGCATTACAAACTGAGTTACAGAAGACAAAGGAGAAGTTACAGGCTGTGGAAGAATTGAAAAGTCAATCAGGTGATGCTGGAACATCGGTGGATTCCTACATATCTGGGAAGATAGTGCAGTTGAAAGAACAAATTGCAACTCTGGAAAAACGTGAAGAAAGGTACAAGACTGTTTTTGCCGACCGAATTTCAGTGTTCAGGAGGGCATGTTGTGAGCTCTTTGGTTACAAGATTGTGATGGATGAACACCAGCGTCCCAATGGAATCCCCATTACACGTTTTACCCTCCAATCTATATATGCGCAAAGTGATGATGAGAAGCTTGAATTTGAATATGAATCAGGGAGCACAAACATTTTGGCAAATGATTACACCTCGCAGTCTGAGATATCTCAACAGGTTGAGATATTTATTCGGAAGTTGAATTCAATCCCAGCTTTCACTGCCAACTTAACAGTGGAGTCTTTCAACCGGCGAACCCTGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000075 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005694 GO:0005819 GO:0005856 GO:0006996 GO:0007049 GO:0007059 GO:0007080 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0012505 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031577 GO:0031967 GO:0031975 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050000 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051315 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0071840 GO:0072686 GO:0098687 GO:0098813 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

ko04110 ko04914 ko05203 map04110 map04914 map05203

Pfam Domains

Protein Families

DUF4094 Galactosyl_T MAD

Protein Analysis

725

Amino Acids

82.39

Weight (kDa)

5.48

Isoelectric Point (pI)

53.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAD	PF05557	110 - 722	1.2e-27	Mitotic checkpoint protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006107)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49880
fragaria_vesca	FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851
malus_domestica	MD08G1248100.v1.1 MD15G1442500.v1.1 MD15G1442900.v1.1
prunus_persica	Prupe.1G585300_v2.0.a1 Prupe.1G585300_v2.0.a1
pyrus_communis	pycom08g21490 pycom15g39070
rosa_chinensis	RchiOBHm_Chr7g0219751
rosa_laevigata	RLG00000002272
rosa_multiflora	Rmu_co8270887.1_g000001 Rmu_sc0007335.1_g000005
rosa_roxburghii	Rroxscaffold_3G00239880
rosa_rugosa	Rorug07G0187600 Rorug07G0187700
rosa_samantha	Rh7AG331300 Rh7BG321100 Rh7CG348700 Rh7DG326800
rosa_wichuraiana	Rw7G027890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1989
AccB1I	GGYRCC	1 cut(s)	43
AccB7I	CCANNNNNTGG	1 cut(s)	80
AciI	CCGC	8 cut(s)	38, 41, 53, 65, 181, 946, 1264, 1655
AclWI	GGATC	1 cut(s)	321
AcsI	RAATTY	5 cut(s)	275, 1088, 1872, 2012, 2110
AcuI	CTGAAG	5 cut(s)	480, 885, 908, 1352, 1370
AcyI	GRCGYC	2 cut(s)	44, 210
AfaI	GTAC	1 cut(s)	1850
AfiI	CCNNNNNNNGG	3 cut(s)	74, 80, 187
AflIII	ACRYGT	1 cut(s)	1961
AjiI	CACGTC	1 cut(s)	359
AjnI	CCWGG	4 cut(s)	73, 79, 1054, 1221
Alw21I	GWGCWC	2 cut(s)	1905, 2036
Alw26I	GTCTC	3 cut(s)	50, 538, 587
AlwI	GGATC	1 cut(s)	321
AlwNI	CAGNNNCTG	2 cut(s)	80, 370
AoxI	GGCC	4 cut(s)	76, 82, 943, 1419
ApoI	RAATTY	5 cut(s)	275, 1088, 1872, 2012, 2110
AseI	ATTAAT	1 cut(s)	1274
AspLEI	GCGC	2 cut(s)	46, 1990
AspS9I	GGNCC	3 cut(s)	77, 83, 943
AsuHPI	GGTGA	4 cut(s)	463, 1148, 1654, 1769
BanI	GGYRCC	1 cut(s)	43
BanII	GRGCYC	1 cut(s)	1905
BbsI	GAAGAC	2 cut(s)	486, 1715
Bbv12I	GWGCWC	2 cut(s)	1905, 2036
BbvCI	CCTCAGC	1 cut(s)	510
BccI	CCATC	1 cut(s)	1918
BcgI	CGANNNNNNTGC	4 cut(s)	645, 679, 1595, 1629
BciT130I	CCWGG	4 cut(s)	75, 81, 1056, 1223
BcoDI	GTCTC	3 cut(s)	50, 538, 587
BfaI	CTAG	3 cut(s)	633, 1175, 1524
BfmI	CTRYAG	1 cut(s)	371
BfoI	RGCGCY	1 cut(s)	47
BglII	AGATCT	2 cut(s)	646, 668
Bme1390I	CCNGG	4 cut(s)	75, 81, 1056, 1223
BmgBI	CACGTC	1 cut(s)	359
BmgT120I	GGNCC	3 cut(s)	77, 83, 943
BmiI	GGNNCC	1 cut(s)	45
BmrFI	CCNGG	4 cut(s)	75, 81, 1056, 1223
BmsI	GCATC	3 cut(s)	850, 1168, 1750
BpiI	GAAGAC	2 cut(s)	486, 1715
BpmI	CTGGAG	3 cut(s)	1184, 1377, 1604
Bpu10I	CCTNAGC	1 cut(s)	510
BpuEI	CTTGAG	2 cut(s)	235, 467
BsaHI	GRCGYC	2 cut(s)	44, 210
BsaI	GGTCTC	1 cut(s)	50
BsaJI	CCNNGG	3 cut(s)	73, 79, 807
BsaXI	ACNNNNNCTCC	2 cut(s)	1879, 1909
Bsc4I	CCNNNNNNNGG	3 cut(s)	74, 80, 187
Bse118I	RCCGGY	1 cut(s)	2157
Bse1I	ACTGG	2 cut(s)	1167, 1371
BseBI	CCWGG	4 cut(s)	75, 81, 1056, 1223
BseDI	CCNNGG	3 cut(s)	73, 79, 807
BseGI	GGATG	3 cut(s)	721, 1246, 1933
BseLI	CCNNNNNNNGG	3 cut(s)	74, 80, 187
BseMII	CTCAG	9 cut(s)	285, 480, 501, 533, 672, 1062, 1284, 1689, 2061
BseNI	ACTGG	2 cut(s)	1167, 1371
BseRI	GAGGAG	4 cut(s)	102, 105, 139, 527
BseYI	CCCAGC	2 cut(s)	600, 2119
BsgI	GTGCAG	2 cut(s)	151, 1823
Bsh1285I	CGRYCG	2 cut(s)	1558, 1870
BshFI	GGCC	4 cut(s)	78, 84, 945, 1421
BshNI	GGYRCC	1 cut(s)	43
BsiEI	CGRYCG	2 cut(s)	1558, 1870
BsiHKAI	GWGCWC	2 cut(s)	1905, 2036
BsiSI	CCGG	2 cut(s)	47, 2158
BslFI	GGGAC	2 cut(s)	369, 1926
BslI	CCNNNNNNNGG	3 cut(s)	74, 80, 187
BsmAI	GTCTC	3 cut(s)	50, 538, 587
BsmBI	CGTCTC	1 cut(s)	587
BsmFI	GGGAC	2 cut(s)	369, 1926
BsmI	GAATGC	1 cut(s)	668
BsnI	GGCC	4 cut(s)	78, 84, 945, 1421
Bso31I	GGTCTC	1 cut(s)	50
Bsp1286I	GDGCHC	2 cut(s)	1905, 2036
Bsp143I	GATC	5 cut(s)	313, 646, 668, 1555, 1572
BspACI	CCGC	8 cut(s)	38, 41, 53, 65, 181, 946, 1264, 1655
BspANI	GGCC	4 cut(s)	78, 84, 945, 1421
BspCNI	CTCAG	9 cut(s)	286, 481, 502, 534, 673, 1063, 1285, 1690, 2062
BspHI	TCATGA	2 cut(s)	406, 898
BspLI	GGNNCC	1 cut(s)	45
BspMAI	CTGCAG	1 cut(s)	375
BspPI	GGATC	1 cut(s)	321
BspQI	GCTCTTC	1 cut(s)	837
BspT107I	GGYRCC	1 cut(s)	43
BspTNI	GGTCTC	1 cut(s)	50
BsrFI	RCCGGY	1 cut(s)	2157
BsrI	ACTGG	2 cut(s)	1167, 1371
BssAI	RCCGGY	1 cut(s)	2157
BssECI	CCNNGG	3 cut(s)	73, 79, 807
BssMI	GATC	5 cut(s)	313, 646, 668, 1555, 1572
BssNI	GRCGYC	2 cut(s)	44, 210
Bst2UI	CCWGG	4 cut(s)	75, 81, 1056, 1223
Bst4CI	ACNGT	3 cut(s)	777, 1858, 2143
Bst6I	CTCTTC	1 cut(s)	837
BstACI	GRCGYC	2 cut(s)	44, 210
BstC8I	GCNNGC	3 cut(s)	69, 234, 746
BstDSI	CCRYGG	1 cut(s)	807
BstENI	CCTNNNNNAGG	1 cut(s)	72
BstF5I	GGATG	3 cut(s)	721, 1246, 1933
BstH2I	RGCGCY	1 cut(s)	47
BstHHI	GCGC	2 cut(s)	46, 1990
BstKTI	GATC	5 cut(s)	316, 649, 671, 1558, 1575
BstMAI	GTCTC	3 cut(s)	50, 538, 587
BstMBI	GATC	5 cut(s)	313, 646, 668, 1555, 1572
BstMCI	CGRYCG	2 cut(s)	1558, 1870
BstMWI	GCNNNNNNNGC	8 cut(s)	50, 379, 506, 844, 1148, 1178, 1223, 2129
BstNI	CCWGG	4 cut(s)	75, 81, 1056, 1223
BstNSI	RCATGY	1 cut(s)	1896
BstSCI	CCNGG	4 cut(s)	73, 79, 1054, 1221
BstSFI	CTRYAG	1 cut(s)	371
BstV2I	GAAGAC	2 cut(s)	486, 1715
BstX2I	RGATCY	2 cut(s)	646, 668
BstYI	RGATCY	2 cut(s)	646, 668
BsuRI	GGCC	4 cut(s)	78, 84, 945, 1421
BtgI	CCRYGG	1 cut(s)	807
BtrI	CACGTC	1 cut(s)	359
BtsCI	GGATG	3 cut(s)	721, 1246, 1933
BtsI	GCAGTG	1 cut(s)	2127
BtsIMutI	CAGTG	3 cut(s)	1884, 2127, 2148
Cac8I	GCNNGC	3 cut(s)	69, 234, 746
CaiI	CAGNNNCTG	2 cut(s)	80, 370
CciI	TCATGA	2 cut(s)	406, 898
CfoI	GCGC	2 cut(s)	46, 1990
Cfr10I	RCCGGY	1 cut(s)	2157
Cfr13I	GGNCC	3 cut(s)	77, 83, 943
CseI	GACGC	3 cut(s)	218, 569, 1928
Csp6I	GTAC	1 cut(s)	1849
CviAII	CATG	7 cut(s)	407, 481, 899, 1065, 1123, 1601, 1893
CviQI	GTAC	1 cut(s)	1849
DinI	GGCGCC	1 cut(s)	45
DpnI	GATC	5 cut(s)	315, 648, 670, 1557, 1574
DpnII	GATC	5 cut(s)	313, 646, 668, 1555, 1572
DraI	TTTAAA	1 cut(s)	267
Eam1104I	CTCTTC	1 cut(s)	837
EarI	CTCTTC	1 cut(s)	837
EciI	GGCGGA	1 cut(s)	68
Ecl136II	GAGCTC	1 cut(s)	1903
Eco147I	AGGCCT	1 cut(s)	1421
Eco24I	GRGCYC	1 cut(s)	1905
Eco31I	GGTCTC	1 cut(s)	50
Eco32I	GATATC	1 cut(s)	2076
Eco53kI	GAGCTC	1 cut(s)	1903
Eco57I	CTGAAG	5 cut(s)	480, 885, 908, 1352, 1370
EcoICRI	GAGCTC	1 cut(s)	1903
EcoNI	CCTNNNNNAGG	1 cut(s)	72
EcoO109I	RGGNCCY	1 cut(s)	77
EcoRI	GAATTC	1 cut(s)	2110
EcoRII	CCWGG	4 cut(s)	73, 79, 1054, 1221
EcoRV	GATATC	1 cut(s)	2076
EcoT38I	GRGCYC	1 cut(s)	1905
EgeI	GGCGCC	1 cut(s)	45
EheI	GGCGCC	1 cut(s)	45
Esp3I	CGTCTC	1 cut(s)	587
FaeI	CATG	7 cut(s)	410, 484, 902, 1068, 1126, 1604, 1896
FalI	AAGNNNNNCTT	2 cut(s)	831, 863
FaqI	GGGAC	2 cut(s)	369, 1926
FatI	CATG	7 cut(s)	406, 480, 898, 1064, 1122, 1600, 1892
FokI	GGATG	3 cut(s)	728, 1253, 1940
FriOI	GRGCYC	1 cut(s)	1905
FspBI	CTAG	3 cut(s)	633, 1175, 1524
FspI	TGCGCA	1 cut(s)	1989
GlaI	GCGC	2 cut(s)	45, 1989
GsaI	CCCAGC	2 cut(s)	604, 2123
GsuI	CTGGAG	3 cut(s)	1184, 1377, 1604
HaeII	RGCGCY	1 cut(s)	47
HaeIII	GGCC	4 cut(s)	78, 84, 945, 1421
HapII	CCGG	2 cut(s)	47, 2158
HgaI	GACGC	3 cut(s)	218, 569, 1928
HhaI	GCGC	2 cut(s)	46, 1990
Hin1I	GRCGYC	2 cut(s)	44, 210
Hin1II	CATG	7 cut(s)	410, 484, 902, 1068, 1126, 1604, 1896
Hin6I	GCGC	2 cut(s)	44, 1988
HinP1I	GCGC	2 cut(s)	44, 1988
HincII	GTYRAC	1 cut(s)	1478
HindII	GTYRAC	1 cut(s)	1478
HindIII	AAGCTT	4 cut(s)	234, 455, 620, 2006
HpaII	CCGG	2 cut(s)	47, 2158
HphI	GGTGA	4 cut(s)	463, 1148, 1654, 1769
Hpy166II	GTNNAC	3 cut(s)	362, 1454, 1478
Hpy8I	GTNNAC	3 cut(s)	362, 1454, 1478
Hpy99I	CGWCG	1 cut(s)	25
HpyAV	CCTTC	6 cut(s)	129, 140, 382, 485, 913, 1228
HpyCH4III	ACNGT	3 cut(s)	777, 1858, 2143
HpyCH4IV	ACGT	4 cut(s)	358, 1480, 1837, 1963
HpyF10VI	GCNNNNNNNGC	8 cut(s)	50, 379, 506, 844, 1148, 1178, 1223, 2129
HpySE526I	ACGT	4 cut(s)	358, 1480, 1837, 1963
Hsp92I	GRCGYC	2 cut(s)	44, 210
Hsp92II	CATG	7 cut(s)	410, 484, 902, 1068, 1126, 1604, 1896
HspAI	GCGC	2 cut(s)	44, 1988
KasI	GGCGCC	1 cut(s)	43
Kzo9I	GATC	5 cut(s)	313, 646, 668, 1555, 1572
LguI	GCTCTTC	1 cut(s)	837
LmnI	GCTCC	2 cut(s)	376, 2031
LweI	GCATC	3 cut(s)	850, 1168, 1750
MaeI	CTAG	3 cut(s)	633, 1175, 1524
MaeII	ACGT	4 cut(s)	358, 1480, 1837, 1963
MaeIII	GTNAC	5 cut(s)	393, 1288, 1701, 1722, 1910
MalI	GATC	5 cut(s)	315, 648, 670, 1557, 1574
MboI	GATC	5 cut(s)	313, 646, 668, 1555, 1572
MflI	RGATCY	2 cut(s)	646, 668
MhlI	GDGCHC	2 cut(s)	1905, 2036
Mly113I	GGCGCC	1 cut(s)	44
MlyI	GAGTC	3 cut(s)	500, 1595, 2156
MmeI	TCCRAC	2 cut(s)	912, 990
MslI	CAYNNNNRTG	1 cut(s)	903
MspA1I	CMGCKG	2 cut(s)	604, 712
MspI	CCGG	2 cut(s)	47, 2158
MspR9I	CCNGG	4 cut(s)	75, 81, 1056, 1223
Mva1269I	GAATGC	1 cut(s)	668
MvaI	CCWGG	4 cut(s)	75, 81, 1056, 1223
MwoI	GCNNNNNNNGC	8 cut(s)	50, 379, 506, 844, 1148, 1178, 1223, 2129
NarI	GGCGCC	1 cut(s)	44
NdeII	GATC	5 cut(s)	313, 646, 668, 1555, 1572
NlaIII	CATG	7 cut(s)	410, 484, 902, 1068, 1126, 1604, 1896
NlaIV	GGNNCC	1 cut(s)	45
NsbI	TGCGCA	1 cut(s)	1989
NspI	RCATGY	1 cut(s)	1896
PagI	TCATGA	2 cut(s)	406, 898
PceI	AGGCCT	1 cut(s)	1421
PciSI	GCTCTTC	1 cut(s)	837
PcsI	WCGNNNNNNNCGW	1 cut(s)	96
PctI	GAATGC	1 cut(s)	668
PflMI	CCANNNNNTGG	1 cut(s)	80
Ple19I	CGATCG	1 cut(s)	1558
PleI	GAGTC	3 cut(s)	499, 1594, 2155
PluTI	GGCGCC	1 cut(s)	47
PpsI	GAGTC	3 cut(s)	499, 1594, 2155
PshBI	ATTAAT	1 cut(s)	1274
Psp124BI	GAGCTC	1 cut(s)	1905
Psp6I	CCWGG	4 cut(s)	73, 79, 1054, 1221
PspFI	CCCAGC	2 cut(s)	600, 2119
PspGI	CCWGG	4 cut(s)	73, 79, 1054, 1221
PspN4I	GGNNCC	1 cut(s)	45
PspPI	GGNCC	3 cut(s)	77, 83, 943
PstI	CTGCAG	1 cut(s)	375
PstNI	CAGNNNCTG	2 cut(s)	80, 370
PsuI	RGATCY	2 cut(s)	646, 668
PvuI	CGATCG	1 cut(s)	1558
PvuII	CAGCTG	2 cut(s)	604, 712
RsaI	GTAC	1 cut(s)	1850
RsaNI	GTAC	1 cut(s)	1849
RseI	CAYNNNNRTG	1 cut(s)	903
SacI	GAGCTC	1 cut(s)	1905
SapI	GCTCTTC	1 cut(s)	837
Sau3AI	GATC	5 cut(s)	313, 646, 668, 1555, 1572
Sau96I	GGNCC	3 cut(s)	77, 83, 943
SchI	GAGTC	3 cut(s)	500, 1595, 2156
ScrFI	CCNGG	4 cut(s)	75, 81, 1056, 1223
SduI	GDGCHC	2 cut(s)	1905, 2036
SfaNI	GCATC	3 cut(s)	850, 1168, 1750
SfcI	CTRYAG	1 cut(s)	371
SfoI	GGCGCC	1 cut(s)	45
SmiMI	CAYNNNNRTG	1 cut(s)	903
SmlI	CTYRAG	2 cut(s)	214, 446
SmoI	CTYRAG	2 cut(s)	214, 446
SseBI	AGGCCT	1 cut(s)	1421
SsiI	CCGC	8 cut(s)	38, 41, 53, 65, 181, 946, 1264, 1655
SspDI	GGCGCC	1 cut(s)	43
SspI	AATATT	1 cut(s)	820
SspMI	CTAG	3 cut(s)	633, 1175, 1524
SstI	GAGCTC	1 cut(s)	1905
StuI	AGGCCT	1 cut(s)	1421
StyD4I	CCNGG	4 cut(s)	73, 79, 1054, 1221
TaaI	ACNGT	3 cut(s)	777, 1858, 2143
TaiI	ACGT	4 cut(s)	361, 1483, 1840, 1966
TaqI	TCGA	2 cut(s)	99, 1558
TaqII	GACCGA	1 cut(s)	1884
TauI	GCSGC	5 cut(s)	41, 44, 183, 948, 1658
TscAI	CASTG	3 cut(s)	1884, 2134, 2148
TspGWI	ACGGA	2 cut(s)	239, 824
TspRI	CASTG	3 cut(s)	1884, 2134, 2148
Van91I	CCANNNNNTGG	1 cut(s)	80
VspI	ATTAAT	1 cut(s)	1274
XagI	CCTNNNNNAGG	1 cut(s)	72
XapI	RAATTY	5 cut(s)	275, 1088, 1872, 2012, 2110
XbaI	TCTAGA	2 cut(s)	632, 1174
XceI	RCATGY	1 cut(s)	1896
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1943, 2140
XspI	CTAG	3 cut(s)	633, 1175, 1524

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.