Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006107

Physical Location & Seq

Genomic Coordinates Forward (+)

54296538 .. 54314624

Gene Structure 18087 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1442900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGATTGTGAGAACTCCCCCAACGAAGAAGCTACGGGCGGCGCCGCCGGAGACCGAGACCCCACCGCCTGCCCCTGCCACAGGCCCAGGCCCTCTCGTCATCTTCGAGGACGACCCTCCTCCTTCCCTTCCACCTCCCGGTTCCTCCTCCGACCACTTGCTCTGCACCTATCAATGCCGCCAAATGGTTAAATCAGATTTTATAGATGCCTTGAGCAATGCAGAGAAGCAAGCTTCTGATTATCAATCTAAATTGGCGGCTTTAAATGAAAAATTCTGCAAAGTTGAATCTGAGAAGAATAAATATCTGGATCAATTTTTGTATGCTGAACAAGAACTTGCCGCTGCAAAGGCACGTGAACAGGATCTGCAGGAGCAGCTTTTGAAGGAGGTGCACGATTCTGATGAAAGATTGACTAAACAATTACAATCAAACAGCAAACTTGAGGTGAAGCTTCAAAACGAAATGAACCTTCGCATGAGAGCTGAGACTTCAACAGGTTTGGCTGAGGAGAAAGCAAGTGTTATAGAGGGGAAACTGAGACATCTTTCTGACAGCATAGAGAAGGAAAAAAAGAGTCTCAATAACAATCTTGCCCAGCTGAAAGGAGAATCCAAGCTCACTGTTTCTAGAATAACTGCAGATCGTGAACGAATGGAATGCAGAGCTCATAATGCTGAGAAAGAAACAGAGCTATTGAAAAAGCAGCTGGATGATATTAAGAAGCAACTTAGTGAGTGCGTGCAACAGAAGAGCGAAGTAGAGAAGAAATTATCAAATCTTACATTTCAAGAAGTTGAATCCACGGAGAATAATATTTTAGTTAAACATCTGCAAGAAGAGCTTAGAAACTTTGATGCTGAAGTAAGGGAAGCAAGAAAGCTGAAGTCAGCTCATGAAAATGTTGAGTTATTGAAGGAAAAATTGTTGGAAGAAAAGAGCCGTAGAGAGAGGGTAGAAGCAGAGTTATCCAAACTACAAGAATTACAGCTTAGTATGAAGAAGTTGGAGGATGAATTGAAATCTTGGAAGTTGATCATTAAAGACATTCCAGGCGTGTCATGTTCTGAGGATTTACCAGTTAAATTCGCATCATTACAAAAAGAGGTAATTGATAGCATGATGAAAGTGAGTGAGGCAAATGCCCACTTGAAACAATTGGAGGTAGCTCTAGATGCAGCAAAAATTGATAAACAAAATGCTGAAATAGAGGCTACACTGGCCAAGGAGAAAGGGGATGTATCAAAATCAGAGGTTAAGCGGATTGAATTGATGCTCTCTATGGTTACTGAGGAAAGAGATAAGTTGAGAAATGTCCTGAATGGACTGAAGTCGGCAAAGAATGATGAAGCAGCCAGTCATACTTTTCTTCAGGAGGTTGAATCATCACTTGCAAAGAAAGAGGGCTATATTAAAGAATTGGAATGCGGTGTATGTGAACAAAAAGAAGTTAATAGTCGTCAACGTGAAGAAATAAAGTTGCTTAATGAGAGGTTGAACAATGAAGCTAGAAGAATAAAGTCATTGGAGAGGGAGAGCGATCGACTTCATTCAGAGATCGCTTTACTGGAGTCCAAGATTGGTCATGGCAATTTCTCTGCTGCTAATACTAAAGTTTTAAGAATGGTGAATACTCTTACGGTTGATAATGAAGCCAAACAAACTATTGAAGCATTACAAACTGAGTTACAGAAGACAAAGGAGAAGTTACAAGCTGTTGAAGAATTGAAAAGTCAATCAGGTGATGCTGGAAAATTGGTGGATTCGTACATATCTGGGAAGATAGTGCAGTTGAAAGAACAAATTGCAACTCTTGAAAAACGTGAAGAAAGGTACAAGACTGTTTTTGCTGACAGAATTTCAGTGTTCAGGAGGGAGTGTTGTGAGCTATTTGGTTACAAGATTGTAATGGATGAACACCAGCGTCCCAATGGAATCCCTGTTACACATTTTACCCTTCAATCTATATATGCGCAAAGTGATGATGAGAAGCTTGAATTTGAATATGAATCAGGGAGCACAAACATTTTGGCAAATGATTACACCTCACAGTCTGAGATATCTCATCAGGTTGAGATATTTATTCGGAAGTTGAATTCAATCCCAGCTTTCACTGCCAACTTAACAGTGGAGTCTTTCAACCGGCGAACCCTGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000075 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005694 GO:0005819 GO:0005856 GO:0006996 GO:0007049 GO:0007059 GO:0007080 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0008608 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0012505 GO:0015630 GO:0016043 GO:0022402 GO:0030071 GO:0031577 GO:0031967 GO:0031975 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050000 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051315 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0071840 GO:0072686 GO:0098687 GO:0098813 GO:0140014 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

ko04110 ko04914 ko05203 map04110 map04914 map05203

Pfam Domains

Protein Families

DUF4094 Galactosyl_T MAD

Protein Analysis

720

Amino Acids

81.86

Weight (kDa)

5.66

Isoelectric Point (pI)

49.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAD	PF05557	108 - 717	8.3e-30	Mitotic checkpoint protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006107)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49880
fragaria_vesca	FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851 FvH4_5g27851
malus_domestica	MD08G1248100.v1.1 MD15G1442500.v1.1 MD15G1442900.v1.1
prunus_persica	Prupe.1G585300_v2.0.a1 Prupe.1G585300_v2.0.a1
pyrus_communis	pycom08g21490 pycom15g39070
rosa_chinensis	RchiOBHm_Chr7g0219751
rosa_laevigata	RLG00000002272
rosa_multiflora	Rmu_co8270887.1_g000001 Rmu_sc0007335.1_g000005
rosa_roxburghii	Rroxscaffold_3G00239880
rosa_rugosa	Rorug07G0187600 Rorug07G0187700
rosa_samantha	Rh7AG331300 Rh7BG321100 Rh7CG348700 Rh7DG326800
rosa_wichuraiana	Rw7G027890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1974
AccB1I	GGYRCC	1 cut(s)	40
AciI	CCGC	8 cut(s)	38, 44, 65, 178, 257, 342, 1261, 1428
AclWI	GGATC	2 cut(s)	318, 372
AcoI	YGGCCR	1 cut(s)	1221
AcsI	RAATTY	5 cut(s)	272, 1085, 1857, 1997, 2095
AcuI	CTGAAG	4 cut(s)	882, 905, 1349, 1355
AcvI	CACGTG	1 cut(s)	356
AcyI	GRCGYC	1 cut(s)	41
AfaI	GTAC	2 cut(s)	1769, 1835
AfiI	CCNNNNNNNGG	3 cut(s)	80, 137, 184
AjnI	CCWGG	2 cut(s)	85, 1051
Alw21I	GWGCWC	3 cut(s)	396, 670, 2021
Alw26I	GTCTC	5 cut(s)	44, 50, 482, 535, 584
Alw44I	GTGCAC	1 cut(s)	392
AlwI	GGATC	2 cut(s)	318, 372
AlwNI	CAGNNNCTG	1 cut(s)	367
AoxI	GGCC	3 cut(s)	82, 88, 1221
ApaLI	GTGCAC	1 cut(s)	392
ApeKI	GCWGC	6 cut(s)	344, 376, 706, 1178, 1352, 1601
ApoI	RAATTY	5 cut(s)	272, 1085, 1857, 1997, 2095
AspLEI	GCGC	2 cut(s)	43, 1975
AspS9I	GGNCC	2 cut(s)	83, 89
AsuC2I	CCSGG	1 cut(s)	138
AsuHPI	GGTGA	3 cut(s)	460, 1639, 1754
BaeGI	GKGCMC	1 cut(s)	396
BalI	TGGCCA	1 cut(s)	1223
BanI	GGYRCC	1 cut(s)	40
BanII	GRGCYC	1 cut(s)	670
BbrPI	CACGTG	1 cut(s)	356
BbsI	GAAGAC	1 cut(s)	1700
Bbv12I	GWGCWC	3 cut(s)	396, 670, 2021
BbvCI	CCTCAGC	1 cut(s)	507
BbvI	GCAGC	6 cut(s)	331, 388, 718, 1190, 1364, 1588
BceAI	ACGGC	1 cut(s)	927
BcgI	CGANNNNNNTGC	2 cut(s)	642, 676
BciT130I	CCWGG	2 cut(s)	87, 1053
BclI	TGATCA	1 cut(s)	1035
BcnI	CCSGG	1 cut(s)	138
BcoDI	GTCTC	5 cut(s)	44, 50, 482, 535, 584
BfaI	CTAG	3 cut(s)	630, 1172, 1509
BfmI	CTRYAG	2 cut(s)	368, 639
BfoI	RGCGCY	1 cut(s)	44
Bme1390I	CCNGG	3 cut(s)	87, 138, 1053
BmgT120I	GGNCC	2 cut(s)	83, 89
BmiI	GGNNCC	2 cut(s)	42, 142
BmrFI	CCNGG	3 cut(s)	87, 138, 1053
BmsI	GCATC	6 cut(s)	196, 847, 1100, 1165, 1263, 1735
BoxI	GACNNNNGTC	1 cut(s)	1330
BpiI	GAAGAC	1 cut(s)	1700
BpmI	CTGGAG	1 cut(s)	1589
Bpu10I	CCTNAGC	1 cut(s)	507
BpuEI	CTTGAG	2 cut(s)	232, 464
BpuMI	CCSGG	1 cut(s)	138
BsaAI	YACGTR	1 cut(s)	356
BsaHI	GRCGYC	1 cut(s)	41
BsaI	GGTCTC	2 cut(s)	44, 50
BsaJI	CCNNGG	3 cut(s)	85, 804, 1224
BsaXI	ACNNNNNCTCC	2 cut(s)	1864, 1894
Bsc4I	CCNNNNNNNGG	3 cut(s)	80, 137, 184
Bse118I	RCCGGY	1 cut(s)	2142
Bse1I	ACTGG	4 cut(s)	1079, 1224, 1356, 1572
Bse3DI	GCAATG	1 cut(s)	223
BseBI	CCWGG	2 cut(s)	87, 1053
BseDI	CCNNGG	3 cut(s)	85, 804, 1224
BseGI	GGATG	4 cut(s)	718, 1018, 1243, 1918
BseLI	CCNNNNNNNGG	3 cut(s)	80, 137, 184
BseMI	GCAATG	1 cut(s)	223
BseMII	CTCAG	9 cut(s)	282, 477, 498, 530, 669, 1059, 1281, 1674, 2046
BseNI	ACTGG	4 cut(s)	1079, 1224, 1356, 1572
BseRI	GAGGAG	3 cut(s)	108, 136, 524
BseSI	GKGCMC	1 cut(s)	396
BseXI	GCAGC	6 cut(s)	331, 388, 718, 1190, 1364, 1588
BseYI	CCCAGC	2 cut(s)	597, 2104
BsgI	GTGCAG	2 cut(s)	148, 1808
Bsh1285I	CGRYCG	1 cut(s)	1543
BshFI	GGCC	3 cut(s)	84, 90, 1223
BshNI	GGYRCC	1 cut(s)	40
BsiEI	CGRYCG	1 cut(s)	1543
BsiHKAI	GWGCWC	3 cut(s)	396, 670, 2021
BsiSI	CCGG	3 cut(s)	47, 138, 2143
BslFI	GGGAC	1 cut(s)	1911
BslI	CCNNNNNNNGG	3 cut(s)	80, 137, 184
BsmAI	GTCTC	5 cut(s)	44, 50, 482, 535, 584
BsmFI	GGGAC	1 cut(s)	1911
BsmI	GAATGC	2 cut(s)	665, 1430
BsnI	GGCC	3 cut(s)	84, 90, 1223
Bso31I	GGTCTC	2 cut(s)	44, 50
Bsp1286I	GDGCHC	3 cut(s)	396, 670, 2021
Bsp143I	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
BspACI	CCGC	8 cut(s)	38, 44, 65, 178, 257, 342, 1261, 1428
BspANI	GGCC	3 cut(s)	84, 90, 1223
BspCNI	CTCAG	9 cut(s)	283, 478, 499, 531, 670, 1060, 1282, 1675, 2047
BspHI	TCATGA	1 cut(s)	895
BspLI	GGNNCC	2 cut(s)	42, 142
BspMAI	CTGCAG	2 cut(s)	372, 643
BspPI	GGATC	2 cut(s)	318, 372
BspQI	GCTCTTC	2 cut(s)	746, 834
BspT107I	GGYRCC	1 cut(s)	40
BspTNI	GGTCTC	2 cut(s)	44, 50
BsrDI	GCAATG	1 cut(s)	223
BsrFI	RCCGGY	1 cut(s)	2142
BsrI	ACTGG	4 cut(s)	1079, 1224, 1356, 1572
BssAI	RCCGGY	1 cut(s)	2142
BssECI	CCNNGG	3 cut(s)	85, 804, 1224
BssMI	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
BssNI	GRCGYC	1 cut(s)	41
BssT1I	CCWWGG	1 cut(s)	1224
Bst2UI	CCWGG	2 cut(s)	87, 1053
Bst4CI	ACNGT	5 cut(s)	625, 1642, 1843, 2052, 2128
Bst6I	CTCTTC	2 cut(s)	746, 834
BstACI	GRCGYC	1 cut(s)	41
BstBAI	YACGTR	1 cut(s)	356
BstC8I	GCNNGC	3 cut(s)	69, 231, 743
BstDSI	CCRYGG	1 cut(s)	804
BstENI	CCTNNNNNAGG	1 cut(s)	78
BstF5I	GGATG	4 cut(s)	718, 1018, 1243, 1918
BstH2I	RGCGCY	1 cut(s)	44
BstHHI	GCGC	2 cut(s)	43, 1975
BstKTI	GATC	6 cut(s)	313, 367, 646, 1038, 1543, 1560
BstMAI	GTCTC	5 cut(s)	44, 50, 482, 535, 584
BstMBI	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
BstMCI	CGRYCG	1 cut(s)	1543
BstMWI	GCNNNNNNNGC	7 cut(s)	350, 376, 674, 841, 1175, 1220, 2114
BstNI	CCWGG	2 cut(s)	87, 1053
BstPAI	GACNNNNGTC	1 cut(s)	1330
BstSCI	CCNGG	3 cut(s)	85, 136, 1051
BstSFI	CTRYAG	2 cut(s)	368, 639
BstSLI	GKGCMC	1 cut(s)	396
BstV1I	GCAGC	6 cut(s)	331, 388, 718, 1190, 1364, 1588
BstV2I	GAAGAC	1 cut(s)	1700
BstX2I	RGATCY	1 cut(s)	364
BstYI	RGATCY	1 cut(s)	364
BsuRI	GGCC	3 cut(s)	84, 90, 1223
BtgI	CCRYGG	1 cut(s)	804
BtsCI	GGATG	4 cut(s)	718, 1018, 1243, 1918
BtsI	GCAGTG	1 cut(s)	2112
BtsIMutI	CAGTG	5 cut(s)	621, 1217, 1869, 2112, 2133
Cac8I	GCNNGC	3 cut(s)	69, 231, 743
CaiI	CAGNNNCTG	1 cut(s)	367
CciI	TCATGA	1 cut(s)	895
CfoI	GCGC	2 cut(s)	43, 1975
Cfr10I	RCCGGY	1 cut(s)	2142
Cfr13I	GGNCC	2 cut(s)	83, 89
CseI	GACGC	1 cut(s)	1913
Csp6I	GTAC	2 cut(s)	1768, 1834
CviAII	CATG	5 cut(s)	478, 896, 1062, 1120, 1586
CviQI	GTAC	2 cut(s)	1768, 1834
DinI	GGCGCC	1 cut(s)	42
DpnI	GATC	6 cut(s)	312, 366, 645, 1037, 1542, 1559
DpnII	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
DraI	TTTAAA	1 cut(s)	264
EaeI	YGGCCR	1 cut(s)	1221
Eam1104I	CTCTTC	2 cut(s)	746, 834
EarI	CTCTTC	2 cut(s)	746, 834
Ecl136II	GAGCTC	1 cut(s)	668
Eco130I	CCWWGG	1 cut(s)	1224
Eco24I	GRGCYC	1 cut(s)	670
Eco31I	GGTCTC	2 cut(s)	44, 50
Eco32I	GATATC	1 cut(s)	2061
Eco53kI	GAGCTC	1 cut(s)	668
Eco57I	CTGAAG	4 cut(s)	882, 905, 1349, 1355
Eco72I	CACGTG	1 cut(s)	356
EcoICRI	GAGCTC	1 cut(s)	668
EcoNI	CCTNNNNNAGG	1 cut(s)	78
EcoO109I	RGGNCCY	1 cut(s)	89
EcoRI	GAATTC	1 cut(s)	2095
EcoRII	CCWGG	2 cut(s)	85, 1051
EcoRV	GATATC	1 cut(s)	2061
EcoT14I	CCWWGG	1 cut(s)	1224
EcoT38I	GRGCYC	1 cut(s)	670
EgeI	GGCGCC	1 cut(s)	42
EheI	GGCGCC	1 cut(s)	42
ErhI	CCWWGG	1 cut(s)	1224
FaeI	CATG	5 cut(s)	481, 899, 1065, 1123, 1589
FalI	AAGNNNNNCTT	2 cut(s)	828, 860
FaqI	GGGAC	1 cut(s)	1911
FatI	CATG	5 cut(s)	477, 895, 1061, 1119, 1585
FbaI	TGATCA	1 cut(s)	1035
FokI	GGATG	4 cut(s)	725, 1025, 1250, 1925
FriOI	GRGCYC	1 cut(s)	670
FspBI	CTAG	3 cut(s)	630, 1172, 1509
FspI	TGCGCA	1 cut(s)	1974
GlaI	GCGC	2 cut(s)	42, 1974
GsaI	CCCAGC	2 cut(s)	601, 2108
GsuI	CTGGAG	1 cut(s)	1589
HaeII	RGCGCY	1 cut(s)	44
HaeIII	GGCC	3 cut(s)	84, 90, 1223
HapII	CCGG	3 cut(s)	47, 138, 2143
HgaI	GACGC	1 cut(s)	1913
HhaI	GCGC	2 cut(s)	43, 1975
Hin1I	GRCGYC	1 cut(s)	41
Hin1II	CATG	5 cut(s)	481, 899, 1065, 1123, 1589
Hin6I	GCGC	2 cut(s)	41, 1973
HinP1I	GCGC	2 cut(s)	41, 1973
HincII	GTYRAC	1 cut(s)	1463
HindII	GTYRAC	1 cut(s)	1463
HindIII	AAGCTT	3 cut(s)	231, 452, 1991
HpaII	CCGG	3 cut(s)	47, 138, 2143
HphI	GGTGA	3 cut(s)	460, 1639, 1754
Hpy166II	GTNNAC	5 cut(s)	359, 394, 650, 1439, 1463
Hpy8I	GTNNAC	5 cut(s)	359, 394, 650, 1439, 1463
HpyAV	CCTTC	7 cut(s)	132, 137, 379, 482, 559, 910, 1967
HpyCH4III	ACNGT	5 cut(s)	625, 1642, 1843, 2052, 2128
HpyCH4IV	ACGT	3 cut(s)	355, 1465, 1822
HpyF10VI	GCNNNNNNNGC	7 cut(s)	350, 376, 674, 841, 1175, 1220, 2114
HpySE526I	ACGT	3 cut(s)	355, 1465, 1822
Hsp92I	GRCGYC	1 cut(s)	41
Hsp92II	CATG	5 cut(s)	481, 899, 1065, 1123, 1589
HspAI	GCGC	2 cut(s)	41, 1973
KasI	GGCGCC	1 cut(s)	40
Ksp22I	TGATCA	1 cut(s)	1035
Kzo9I	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
LguI	GCTCTTC	2 cut(s)	746, 834
LmnI	GCTCC	2 cut(s)	373, 2016
Lsp1109I	GCAGC	6 cut(s)	331, 388, 718, 1190, 1364, 1588
LweI	GCATC	6 cut(s)	196, 847, 1100, 1165, 1263, 1735
MaeI	CTAG	3 cut(s)	630, 1172, 1509
MaeII	ACGT	3 cut(s)	355, 1465, 1822
MaeIII	GTNAC	5 cut(s)	1285, 1686, 1707, 1895, 1942
MalI	GATC	6 cut(s)	312, 366, 645, 1037, 1542, 1559
MboI	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
MfeI	CAATTG	1 cut(s)	1157
MflI	RGATCY	1 cut(s)	364
MhlI	GDGCHC	3 cut(s)	396, 670, 2021
MlsI	TGGCCA	1 cut(s)	1223
MluNI	TGGCCA	1 cut(s)	1223
Mly113I	GGCGCC	1 cut(s)	41
MlyI	GAGTC	3 cut(s)	586, 1580, 2141
MmeI	TCCRAC	3 cut(s)	174, 909, 987
Mox20I	TGGCCA	1 cut(s)	1223
MscI	TGGCCA	1 cut(s)	1223
MslI	CAYNNNNRTG	1 cut(s)	900
Msp20I	TGGCCA	1 cut(s)	1223
MspA1I	CMGCKG	3 cut(s)	344, 601, 709
MspI	CCGG	3 cut(s)	47, 138, 2143
MspR9I	CCNGG	3 cut(s)	87, 138, 1053
MunI	CAATTG	1 cut(s)	1157
Mva1269I	GAATGC	2 cut(s)	665, 1430
MvaI	CCWGG	2 cut(s)	87, 1053
MwoI	GCNNNNNNNGC	7 cut(s)	350, 376, 674, 841, 1175, 1220, 2114
NarI	GGCGCC	1 cut(s)	41
NciI	CCSGG	1 cut(s)	138
NdeII	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
NlaIII	CATG	5 cut(s)	481, 899, 1065, 1123, 1589
NlaIV	GGNNCC	2 cut(s)	42, 142
NsbI	TGCGCA	1 cut(s)	1974
PagI	TCATGA	1 cut(s)	895
PciSI	GCTCTTC	2 cut(s)	746, 834
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PctI	GAATGC	2 cut(s)	665, 1430
PfeI	GAWTC	8 cut(s)	287, 398, 611, 800, 1382, 1763, 1935, 2009
Ple19I	CGATCG	1 cut(s)	1543
PleI	GAGTC	3 cut(s)	585, 1579, 2140
PluTI	GGCGCC	1 cut(s)	44
PmaCI	CACGTG	1 cut(s)	356
PmlI	CACGTG	1 cut(s)	356
PpsI	GAGTC	3 cut(s)	585, 1579, 2140
Ppu21I	YACGTR	1 cut(s)	356
PshAI	GACNNNNGTC	1 cut(s)	1330
Psp124BI	GAGCTC	1 cut(s)	670
Psp6I	CCWGG	2 cut(s)	85, 1051
PspCI	CACGTG	1 cut(s)	356
PspFI	CCCAGC	2 cut(s)	597, 2104
PspGI	CCWGG	2 cut(s)	85, 1051
PspN4I	GGNNCC	2 cut(s)	42, 142
PspPI	GGNCC	2 cut(s)	83, 89
PstI	CTGCAG	2 cut(s)	372, 643
PstNI	CAGNNNCTG	1 cut(s)	367
PsuI	RGATCY	1 cut(s)	364
PvuI	CGATCG	1 cut(s)	1543
PvuII	CAGCTG	2 cut(s)	601, 709
RsaI	GTAC	2 cut(s)	1769, 1835
RsaNI	GTAC	2 cut(s)	1768, 1834
RseI	CAYNNNNRTG	1 cut(s)	900
SacI	GAGCTC	1 cut(s)	670
SapI	GCTCTTC	2 cut(s)	746, 834
Sau3AI	GATC	6 cut(s)	310, 364, 643, 1035, 1540, 1557
Sau96I	GGNCC	2 cut(s)	83, 89
SchI	GAGTC	3 cut(s)	586, 1580, 2141
ScrFI	CCNGG	3 cut(s)	87, 138, 1053
SduI	GDGCHC	3 cut(s)	396, 670, 2021
SfaNI	GCATC	6 cut(s)	196, 847, 1100, 1165, 1263, 1735
SfcI	CTRYAG	2 cut(s)	368, 639
SfoI	GGCGCC	1 cut(s)	42
SmiMI	CAYNNNNRTG	1 cut(s)	900
SmlI	CTYRAG	2 cut(s)	211, 443
SmoI	CTYRAG	2 cut(s)	211, 443
SsiI	CCGC	8 cut(s)	38, 44, 65, 178, 257, 342, 1261, 1428
SspDI	GGCGCC	1 cut(s)	40
SspI	AATATT	1 cut(s)	817
SspMI	CTAG	3 cut(s)	630, 1172, 1509
SstI	GAGCTC	1 cut(s)	670
StyD4I	CCNGG	3 cut(s)	85, 136, 1051
StyI	CCWWGG	1 cut(s)	1224
TaaI	ACNGT	5 cut(s)	625, 1642, 1843, 2052, 2128
TaiI	ACGT	3 cut(s)	358, 1468, 1825
TaqI	TCGA	2 cut(s)	105, 1543
TaqII	GACCGA	1 cut(s)	68
TauI	GCSGC	5 cut(s)	41, 46, 180, 260, 344
TfiI	GAWTC	8 cut(s)	287, 398, 611, 800, 1382, 1763, 1935, 2009
TscAI	CASTG	5 cut(s)	628, 1224, 1869, 2119, 2133
TseI	GCWGC	6 cut(s)	344, 376, 706, 1178, 1352, 1601
TspGWI	ACGGA	1 cut(s)	821
TspRI	CASTG	5 cut(s)	628, 1224, 1869, 2119, 2133
VneI	GTGCAC	1 cut(s)	392
XagI	CCTNNNNNAGG	1 cut(s)	78
XapI	RAATTY	5 cut(s)	272, 1085, 1857, 1997, 2095
XbaI	TCTAGA	2 cut(s)	629, 1171
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1928, 2125
XspI	CTAG	3 cut(s)	630, 1172, 1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.