MD09G1000500.v1.1

heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005956
Physical Location & Seq
Reverse (-)
118090 .. 118527
438 bp
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UTR
Exon/CDS
Intron
MD09G1000500.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGCGGAGGCTGAGACGTTTGCCTTCCAGGCCGAGGTCAACCAACTTCTGAGTTTGATCATCAACACCTTCTACAGCAACAAGGAGATCTTTCTTCGTAAGCTCATCAGCGATGCCTCTGATGCTCTTAATAAGATCAGATTTGAAAGCTTGACCGACAAGAGCAAACTCGATGGTCAGCCAGAGCTTTTCATCTATATCATCCCTGACAAAGCCAACAATACATTGACCAAAATCGACAGTGGTATTGGGATGACCAAAGCTGATTTAGTGAACAACCTTGGTACCATAGCTAGGTCTGGCACCAAGGAATTCATGGAAGCCTTGGCTGCTAGTGCTGATGTTAGCATGATCGGTCAGTTCGGTGTTGGGTTCTACTCCATATACCTTGTTGCCGATAAGGTCATTGTTACCACCAAGCACAATGACGATAAGTAG

Protein Analysis

146

Amino Acids

15.98

Weight (kDa)

4.97

Isoelectric Point (pI)

19.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HATPase_c_3 PF13589 68 - 144 9.4e-06 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 284, 302
AciI CCGC 1 cut(s) 5
AcsI RAATTY 1 cut(s) 311
AfaI GTAC 1 cut(s) 286
AfiI CCNNNNNNNGG 2 cut(s) 34, 294
AgsI TTSAA 1 cut(s) 146
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 5 cut(s) 103, 150, 187, 263, 293
AluI AGCT 5 cut(s) 103, 150, 187, 263, 293
Alw26I GTCTC 1 cut(s) 8
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 1 cut(s) 329
ApoI RAATTY 1 cut(s) 311
Asp718I GGTACC 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 268, 301
BanI GGYRCC 2 cut(s) 284, 302
BbvI GCAGC 1 cut(s) 316
BccI CCATC 1 cut(s) 167
BciT130I CCWGG 1 cut(s) 29
BclI TGATCA 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 2 cut(s) 294, 333
BfmI CTRYAG 1 cut(s) 73
BglI GCCNNNNNGGC 1 cut(s) 29
BglII AGATCT 1 cut(s) 87
BisI GCNGC 1 cut(s) 330
BlsI GCNGC 1 cut(s) 331
Bme1390I CCNGG 1 cut(s) 29
BmiI GGNNCC 2 cut(s) 286, 304
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 2 cut(s) 103, 112
BsaJI CCNNGG 4 cut(s) 33, 280, 306, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 294
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 4 cut(s) 33, 280, 306, 324
BseGI GGATG 2 cut(s) 201, 258
BseLI CCNNNNNNNGG 2 cut(s) 34, 294
BseMII CTCAG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 2 cut(s) 284, 302
BslI CCNNNNNNNGG 2 cut(s) 34, 294
BsmAI GTCTC 1 cut(s) 8
BsmBI CGTCTC 1 cut(s) 8
BsnI GGCC 1 cut(s) 32
Bsp143I GATC 4 cut(s) 57, 87, 135, 351
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 4, 42
BspLI GGNNCC 2 cut(s) 286, 304
BspT107I GGYRCC 2 cut(s) 284, 302
BssECI CCNNGG 4 cut(s) 33, 280, 306, 324
BssMI GATC 4 cut(s) 57, 87, 135, 351
BssT1I CCWWGG 3 cut(s) 280, 306, 324
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 12, 50
BstF5I GGATG 2 cut(s) 201, 258
BstKTI GATC 4 cut(s) 60, 90, 138, 354
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 4 cut(s) 57, 87, 135, 351
BstMWI GCNNNNNNNGC 4 cut(s) 29, 122, 329, 335
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 1 cut(s) 73
BstV1I GCAGC 1 cut(s) 316
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BsuRI GGCC 1 cut(s) 32
BtgZI GCGATG 1 cut(s) 126
BtsCI GGATG 2 cut(s) 201, 258
BtsIMutI CAGTG 1 cut(s) 247
Csp6I GTAC 1 cut(s) 285
CviAII CATG 2 cut(s) 316, 349
CviQI GTAC 1 cut(s) 285
DdeI CTNAG 2 cut(s) 12, 50
DpnI GATC 4 cut(s) 59, 89, 137, 353
DpnII GATC 4 cut(s) 57, 87, 135, 351
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 280, 306, 324
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 3 cut(s) 280, 306, 324
ErhI CCWWGG 3 cut(s) 280, 306, 324
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 2 cut(s) 319, 352
FaiI YATR 6 cut(s) 198, 290, 317, 350, 383, 385
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FatI CATG 2 cut(s) 315, 348
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 1 cut(s) 330
FokI GGATG 2 cut(s) 188, 265
Fsp4HI GCNGC 1 cut(s) 330
FspBI CTAG 2 cut(s) 294, 333
GluI GCNGC 1 cut(s) 330
HaeIII GGCC 1 cut(s) 32
Hin1II CATG 2 cut(s) 319, 352
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 148
Hpy166II GTNNAC 2 cut(s) 40, 274
Hpy188I TCNGA 3 cut(s) 51, 121, 140
Hpy8I GTNNAC 2 cut(s) 40, 274
HpyAV CCTTC 2 cut(s) 34, 79
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 122, 329, 335
HpyF3I CTNAG 2 cut(s) 12, 50
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 2 cut(s) 319, 352
KpnI GGTACC 1 cut(s) 288
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 4 cut(s) 57, 87, 135, 351
LpnPI CCDG 5 cut(s) 14, 41, 195, 219, 285
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 2 cut(s) 103, 112
MaeI CTAG 2 cut(s) 294, 333
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 1 cut(s) 409
MalI GATC 4 cut(s) 59, 89, 137, 353
MboI GATC 4 cut(s) 57, 87, 135, 351
MboII GAAGA 1 cut(s) 86
MflI RGATCY 1 cut(s) 87
MluCI AATT 1 cut(s) 311
MnlI CCTC 2 cut(s) 28, 127
MseI TTAA 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 29
MvaI CCWGG 1 cut(s) 29
MwoI GCNNNNNNNGC 4 cut(s) 29, 122, 329, 335
NdeII GATC 4 cut(s) 57, 87, 135, 351
NlaIII CATG 2 cut(s) 319, 352
NlaIV GGNNCC 2 cut(s) 286, 304
NmeAIII GCCGAG 1 cut(s) 58
PkrI GCNGC 1 cut(s) 331
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 2 cut(s) 286, 304
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 1 cut(s) 286
RsaNI GTAC 1 cut(s) 285
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 1 cut(s) 330
Sau3AI GATC 4 cut(s) 57, 87, 135, 351
ScrFI CCNGG 1 cut(s) 29
SfaNI GCATC 2 cut(s) 103, 112
SfcI CTRYAG 1 cut(s) 73
Sse9I AATT 1 cut(s) 311
SsiI CCGC 1 cut(s) 5
SspMI CTAG 2 cut(s) 294, 333
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 3 cut(s) 280, 306, 324
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 20
TaqI TCGA 2 cut(s) 171, 237
TaqII GACCGA 2 cut(s) 170, 344
TasI AATT 1 cut(s) 311
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 1 cut(s) 247
TseI GCWGC 1 cut(s) 329
TspDTI ATGAA 2 cut(s) 181, 304
TspRI CASTG 1 cut(s) 247
XapI RAATTY 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XspI CTAG 2 cut(s) 294, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.