pycom03g02800

heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0005956
Physical Location & Seq
Reverse (-)
2199220 .. 2200515
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02800.1

Sequence Viewer

Length: 1296 bp
ATGAGGAAGCCAGAGGAGATCACCAAGGAAGAGTACTCCGCATTCTACAAGAGTCTCACCAATGACTGGGAGGAGCATCTTTCTGTGAAGCACTTTTCAGTTGAGGGTCAGCTTGAGTTCAAGGCCATCCTCTTTGTTCCCAAGAGGGCTCCATTCGATCTGTTTGACACCAGGAAGAAGCCCAACAACATCAAGCTCTATGTGCGCCGTGTCTTTATCATGGACAACTGTGAGGAGTTGATGCCCGAATACCTTGGCTTTGTCAAGGGTATTGTCGACTCTGAGGATCTTCCTCTTAACATCTCAAGAGAAATGTTGCAGCAGAACAAGATCTTGAAGGTCATCCGCAAGAACTTGGTTAAGAAATGCATTGAGATGTTCCAGGAAATTGCTGAGAACAAGGAGGACTACAACAAGTTCTACGAGTCATTCTCCAAGAACCTTAAGCTCGGAATCCATGAAGACTCCCAGAACAAGACCAAGCTTGCTGAGTTGTTGAGATACCACTCCACCAAGAGCGGAGAGGAGATGACCAGCCTGAAGGACTATGTCACAAGGATGAAGGAGGGCCAGCAAGACATCTACTATATTACTGGTGAGAGCAAGAAGGCTGTCGAGAACTCTCCATTCCTCGAGAAGCTGAAGAAGAAGGGATTTGAGGTTCTCTTCATGGTTGATGCCATTGATGAGTATGCTATTGGTCAGCTCAAGGAGTTCGAGGGCAAGAAGCTTGTCTCAGCCACCAAAGAAGGTTTGAAGCTTGACGAGAGTGAAGATGAGGCCAAGAAGAAGGAAGAGCTCAAGGAGAAGTTTGAGGGACTCTGCAAGGTCGTCAAGGATGTCTTGGGCGACAGAGTCGAGAAGGTGGTTGTGTCTGACCGTGTGGTCGATTCTCCCTGCTGTTTGGTGACCGGCGAGTATGGCTGGACTGCTAACATGGAAAGAATCATGAAGGCTCAGGCTTTGAGGGATAACAGTATGGCTGGATACATGTCTTCCAAGAAGACCATGGAGATCAACCCAGAGAACCCAATCATGGAGGAGCTTAGGAAGAGGTCTGATGCAGACAAGAACGACAAGTCGGTGAAGGACCTTGTCCTCCTCCTGTTTGAGACTGCTCTTCTCACCTCCGGGTTCAGCCTCGACGACCCCAACACCTTCGGCAACAGGATCCACAGGATGTTGAAGCTCGGACTGAGCATTGATGATGATGCCGTTGAGGGCGACGCTGAGATGCCACCATTGGAGGACGCTGATGCCGATGCAGAGGGCAGCAAGATGGAGGAAGTCGATTAA

Protein Analysis

432

Amino Acids

49.62

Weight (kDa)

5.13

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP90 PF00183 1 - 414 4.6e-209 Hsp90 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 884
AccB7I CCANNNNNTGG 1 cut(s) 66
AccBSI CCGCTC 1 cut(s) 519
AccI GTMKAC 1 cut(s) 276
AciI CCGC 3 cut(s) 39, 346, 519
AclWI GGATC 3 cut(s) 294, 1165, 1178
AcuI CTGAAG 2 cut(s) 560, 662
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 2 cut(s) 66, 1036
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 4 cut(s) 121, 337, 757, 1186
AjnI CCWGG 2 cut(s) 170, 381
AloI GAACNNNNNNTCC 2 cut(s) 645, 677
Alw21I GWGCWC 1 cut(s) 801
Alw26I GTCTC 3 cut(s) 59, 739, 1106
AlwI GGATC 3 cut(s) 294, 1165, 1178
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 3 cut(s) 123, 568, 780
ApeKI GCWGC 2 cut(s) 319, 1272
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 568, 1090
AsuC2I CCSGG 1 cut(s) 1132
AsuHPI GGTGA 6 cut(s) 13, 49, 608, 919, 1096, 1117
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 1 cut(s) 1090
BamHI GGATCC 1 cut(s) 1170
BanII GRGCYC 2 cut(s) 151, 801
BbsI GAAGAC 3 cut(s) 468, 987, 1010
Bbv12I GWGCWC 1 cut(s) 801
BbvI GCAGC 2 cut(s) 331, 1284
BccI CCATC 2 cut(s) 134, 1273
BceAI ACGGC 2 cut(s) 192, 1199
BciT130I CCWGG 2 cut(s) 172, 383
BciVI GTATCC 1 cut(s) 980
BcnI CCSGG 1 cut(s) 1132
BcoDI GTCTC 3 cut(s) 59, 739, 1106
BfrI CTTAAG 1 cut(s) 443
BfuI GTATCC 1 cut(s) 980
BglII AGATCT 1 cut(s) 330
BisI GCNGC 2 cut(s) 320, 1273
BlsI GCNGC 2 cut(s) 321, 1274
BmcAI AGTACT 1 cut(s) 35
Bme1390I CCNGG 3 cut(s) 172, 383, 1132
Bme18I GGWCC 1 cut(s) 1090
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 568, 1090
BmiI GGNNCC 2 cut(s) 150, 1172
BmrFI CCNGG 3 cut(s) 172, 383, 1132
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 8 cut(s) 85, 231, 667, 1051, 1201, 1224, 1246, 1252
BmuI ACTGGG 1 cut(s) 76
BpiI GAAGAC 3 cut(s) 468, 987, 1010
BplI GAGNNNNNCTC 2 cut(s) 416, 448
Bpu10I CCTNAGC 2 cut(s) 957, 1046
BpuEI CTTGAG 4 cut(s) 134, 289, 692, 785
BpuMI CCSGG 1 cut(s) 1132
BsaJI CCNNGG 3 cut(s) 24, 253, 1008
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 1036
Bse118I RCCGGY 1 cut(s) 911
Bse1I ACTGG 2 cut(s) 71, 598
BseBI CCWGG 2 cut(s) 172, 383
BseDI CCNNGG 3 cut(s) 24, 253, 1008
BseGI GGATG 5 cut(s) 126, 342, 564, 844, 1185
BseLI CCNNNNNNNGG 2 cut(s) 66, 1036
BseMII CTCAG 7 cut(s) 273, 384, 480, 750, 971, 1187, 1221
BseNI ACTGG 2 cut(s) 71, 598
BseRI GAGGAG 6 cut(s) 29, 86, 248, 539, 1055, 1091
BseXI GCAGC 2 cut(s) 331, 1284
BshFI GGCC 3 cut(s) 125, 570, 782
BsiHKAI GWGCWC 1 cut(s) 801
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 2 cut(s) 912, 1131
BslFI GGGAC 1 cut(s) 831
BslI CCNNNNNNNGG 2 cut(s) 66, 1036
BsmAI GTCTC 3 cut(s) 59, 739, 1106
BsmFI GGGAC 1 cut(s) 831
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 3 cut(s) 125, 570, 782
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 2 cut(s) 151, 801
Bsp143I GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
Bsp19I CCATGG 1 cut(s) 1008
BspACI CCGC 3 cut(s) 39, 346, 519
BspANI GGCC 3 cut(s) 125, 570, 782
BspCNI CTCAG 7 cut(s) 274, 385, 481, 749, 970, 1188, 1222
BspHI TCATGA 1 cut(s) 948
BspLI GGNNCC 2 cut(s) 150, 1172
BspPI GGATC 3 cut(s) 294, 1165, 1178
BspQI GCTCTTC 2 cut(s) 789, 1125
BspTI CTTAAG 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 519
BsrFI RCCGGY 1 cut(s) 911
BsrI ACTGG 2 cut(s) 71, 598
BssAI RCCGGY 1 cut(s) 911
BssECI CCNNGG 3 cut(s) 24, 253, 1008
BssMI GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
BssT1I CCWWGG 3 cut(s) 24, 253, 1008
Bst2UI CCWGG 2 cut(s) 172, 383
Bst4CI ACNGT 3 cut(s) 230, 881, 977
Bst6I CTCTTC 5 cut(s) 24, 671, 789, 1046, 1125
BstAFI CTTAAG 1 cut(s) 443
BstC8I GCNNGC 2 cut(s) 486, 572
BstDEI CTNAG 8 cut(s) 282, 393, 489, 736, 957, 1046, 1196, 1230
BstDSI CCRYGG 1 cut(s) 1008
BstEII GGTNACC 1 cut(s) 907
BstF5I GGATG 5 cut(s) 126, 342, 564, 844, 1185
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 6 cut(s) 21, 160, 289, 333, 1017, 1173
BstMAI GTCTC 3 cut(s) 59, 739, 1106
BstMBI GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
BstMWI GCNNNNNNNGC 2 cut(s) 202, 921
BstNI CCWGG 2 cut(s) 172, 383
BstNSI RCATGY 1 cut(s) 994
BstPI GGTNACC 1 cut(s) 907
BstSCI CCNGG 3 cut(s) 170, 381, 1130
BstV1I GCAGC 2 cut(s) 331, 1284
BstV2I GAAGAC 3 cut(s) 468, 987, 1010
BstX2I RGATCY 3 cut(s) 286, 330, 1170
BstYI RGATCY 3 cut(s) 286, 330, 1170
BsuI GTATCC 1 cut(s) 980
BsuRI GGCC 3 cut(s) 125, 570, 782
BtgI CCRYGG 1 cut(s) 1008
BtsCI GGATG 5 cut(s) 126, 342, 564, 844, 1185
Cac8I GCNNGC 2 cut(s) 486, 572
CciI TCATGA 1 cut(s) 948
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 911
Cfr13I GGNCC 2 cut(s) 568, 1090
CseI GACGC 2 cut(s) 1235, 1259
Csp6I GTAC 1 cut(s) 34
CviAII CATG 8 cut(s) 220, 458, 670, 937, 949, 991, 1009, 1036
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 8 cut(s) 282, 393, 489, 736, 957, 1046, 1196, 1230
DpnI GATC 6 cut(s) 20, 159, 288, 332, 1016, 1172
DpnII GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
DrdI GACNNNNNNGTC 1 cut(s) 884
DseDI GACNNNNNNGTC 1 cut(s) 884
Eam1104I CTCTTC 5 cut(s) 24, 671, 789, 1046, 1125
EarI CTCTTC 5 cut(s) 24, 671, 789, 1046, 1125
Ecl136II GAGCTC 1 cut(s) 799
Eco130I CCWWGG 3 cut(s) 24, 253, 1008
Eco24I GRGCYC 2 cut(s) 151, 801
Eco47I GGWCC 1 cut(s) 1090
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 2 cut(s) 560, 662
Eco88I CYCGRG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 907
EcoICRI GAGCTC 1 cut(s) 799
EcoO109I RGGNCCY 1 cut(s) 1090
EcoO65I GGTNACC 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 170, 381
EcoT14I CCWWGG 3 cut(s) 24, 253, 1008
EcoT22I ATGCAT 1 cut(s) 371
EcoT38I GRGCYC 2 cut(s) 151, 801
ErhI CCWWGG 3 cut(s) 24, 253, 1008
FaeI CATG 8 cut(s) 223, 461, 673, 940, 952, 994, 1012, 1039
FalI AAGNNNNNCTT 2 cut(s) 827, 859
FaqI GGGAC 1 cut(s) 831
FatI CATG 8 cut(s) 219, 457, 669, 936, 948, 990, 1008, 1035
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 320, 1273
FokI GGATG 5 cut(s) 113, 329, 571, 851, 1192
FriOI GRGCYC 2 cut(s) 151, 801
Fsp4HI GCNGC 2 cut(s) 320, 1273
GlaI GCGC 1 cut(s) 206
GluI GCNGC 2 cut(s) 320, 1273
HaeIII GGCC 3 cut(s) 125, 570, 782
HapII CCGG 2 cut(s) 912, 1131
HgaI GACGC 2 cut(s) 1235, 1259
HhaI GCGC 1 cut(s) 207
Hin1II CATG 8 cut(s) 223, 461, 673, 940, 952, 994, 1012, 1039
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 3 cut(s) 482, 728, 758
HinfI GANTC 9 cut(s) 52, 278, 425, 453, 464, 819, 855, 890, 945
HpaII CCGG 2 cut(s) 912, 1131
HphI GGTGA 6 cut(s) 13, 49, 608, 919, 1096, 1117
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 5 cut(s) 283, 452, 877, 1060, 1193
Hpy188III TCNNGA 6 cut(s) 306, 334, 616, 634, 859, 949
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 2 cut(s) 1148, 1229
HpyCH4III ACNGT 3 cut(s) 230, 881, 977
HpyCH4V TGCA 5 cut(s) 319, 369, 825, 1064, 1265
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 921
HpyF3I CTNAG 8 cut(s) 282, 393, 489, 736, 957, 1046, 1196, 1230
Hsp92II CATG 8 cut(s) 223, 461, 673, 940, 952, 994, 1012, 1039
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
LguI GCTCTTC 2 cut(s) 789, 1125
LmnI GCTCC 3 cut(s) 73, 154, 1042
Lsp1109I GCAGC 2 cut(s) 331, 1284
LweI GCATC 8 cut(s) 85, 231, 667, 1051, 1201, 1224, 1246, 1252
MaeIII GTNAC 2 cut(s) 550, 907
MalI GATC 6 cut(s) 20, 159, 288, 332, 1016, 1172
MbiI CCGCTC 1 cut(s) 519
MboI GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
MflI RGATCY 3 cut(s) 286, 330, 1170
MhlI GDGCHC 2 cut(s) 151, 801
MluCI AATT 1 cut(s) 387
MlyI GAGTC 6 cut(s) 61, 272, 434, 458, 813, 864
Mph1103I ATGCAT 1 cut(s) 371
MseI TTAA 4 cut(s) 297, 360, 444, 1294
MslI CAYNNNNRTG 2 cut(s) 374, 557
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 2 cut(s) 912, 1131
MspR9I CCNGG 3 cut(s) 172, 383, 1132
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 2 cut(s) 172, 383
MwoI GCNNNNNNNGC 2 cut(s) 202, 921
NciI CCSGG 1 cut(s) 1132
NcoI CCATGG 1 cut(s) 1008
NdeII GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
NlaIII CATG 8 cut(s) 223, 461, 673, 940, 952, 994, 1012, 1039
NlaIV GGNNCC 2 cut(s) 150, 1172
NmuCI GTSAC 2 cut(s) 550, 907
NsiI ATGCAT 1 cut(s) 371
NspI RCATGY 1 cut(s) 994
PaeR7I CTCGAG 1 cut(s) 632
PagI TCATGA 1 cut(s) 948
PciI ACATGT 1 cut(s) 990
PciSI GCTCTTC 2 cut(s) 789, 1125
PcsI WCGNNNNNNNCGW 1 cut(s) 1257
PctI GAATGC 1 cut(s) 41
PfeI GAWTC 3 cut(s) 453, 890, 945
PflFI GACNNNGTC 3 cut(s) 548, 854, 1094
PflMI CCANNNNNTGG 1 cut(s) 66
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 2 cut(s) 321, 1274
PleI GAGTC 6 cut(s) 60, 272, 433, 458, 813, 863
PpsI GAGTC 6 cut(s) 60, 272, 433, 458, 813, 863
PpuMI RGGWCCY 1 cut(s) 1090
PscI ACATGT 1 cut(s) 990
Psp124BI GAGCTC 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 1090
Psp6I CCWGG 2 cut(s) 170, 381
PspEI GGTNACC 1 cut(s) 907
PspGI CCWGG 2 cut(s) 170, 381
PspN4I GGNNCC 2 cut(s) 150, 1172
PspPI GGNCC 2 cut(s) 568, 1090
PspPPI RGGWCCY 1 cut(s) 1090
PsuI RGATCY 3 cut(s) 286, 330, 1170
PsyI GACNNNGTC 3 cut(s) 548, 854, 1094
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 2 cut(s) 374, 557
SacI GAGCTC 1 cut(s) 801
SalI GTCGAC 1 cut(s) 275
SapI GCTCTTC 2 cut(s) 789, 1125
SaqAI TTAA 4 cut(s) 297, 360, 444, 1294
SatI GCNGC 2 cut(s) 320, 1273
Sau3AI GATC 6 cut(s) 18, 157, 286, 330, 1014, 1170
Sau96I GGNCC 2 cut(s) 568, 1090
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 6 cut(s) 61, 272, 434, 458, 813, 864
ScrFI CCNGG 3 cut(s) 172, 383, 1132
SduI GDGCHC 2 cut(s) 151, 801
SfaNI GCATC 8 cut(s) 85, 231, 667, 1051, 1201, 1224, 1246, 1252
Sfr274I CTCGAG 1 cut(s) 632
SinI GGWCC 1 cut(s) 1090
SlaI CTCGAG 1 cut(s) 632
SmiMI CAYNNNNRTG 2 cut(s) 374, 557
SmlI CTYRAG 6 cut(s) 113, 304, 443, 632, 707, 800
SmoI CTYRAG 6 cut(s) 113, 304, 443, 632, 707, 800
Sse9I AATT 1 cut(s) 387
SsiI CCGC 3 cut(s) 39, 346, 519
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 3 cut(s) 170, 381, 1130
StyI CCWWGG 3 cut(s) 24, 253, 1008
TaaI ACNGT 3 cut(s) 230, 881, 977
TaqI TCGA 9 cut(s) 156, 276, 615, 633, 717, 858, 888, 1143, 1290
TasI AATT 1 cut(s) 387
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 3 cut(s) 453, 890, 945
Tru1I TTAA 4 cut(s) 297, 360, 444, 1294
Tru9I TTAA 4 cut(s) 297, 360, 444, 1294
TseFI GTSAC 2 cut(s) 550, 907
TseI GCWGC 2 cut(s) 319, 1272
Tsp45I GTSAC 2 cut(s) 550, 907
TspDTI ATGAA 4 cut(s) 474, 575, 658, 965
Tth111I GACNNNGTC 3 cut(s) 548, 854, 1094
Van91I CCANNNNNTGG 1 cut(s) 66
Vha464I CTTAAG 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 1090
XceI RCATGY 1 cut(s) 994
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XhoI CTCGAG 1 cut(s) 632
XmiI GTMKAC 1 cut(s) 276
ZrmI AGTACT 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.