Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010488

Physical Location & Seq

Genomic Coordinates Forward (+)

1308801 .. 1312152

Gene Structure 3352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1021500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1905 bp

ATGAAGCACAATGGCCAGCTCAGTTCTTTCCCCGCTGCGAACAAGCTGCTGAAGTTCGCTATAATCGTTCTCATTGCCTGCCTGGGCTTGCTCTGTTTGTACTACGGGTGTTCTTTCGTGCCGGGCTCGCGGAGATCCGATGAAGAAGCTTCCGGCTCCGATGGACTCCACCCGCTTTTCGGTGGGTTCGTTCTCCATCGCGATTTGGATGATTTGCATGACGATCAGGAGCACAACTCTGAGATCCTTAAAAGCTTGCCTATATGTGATTTACGGTTTTCAGAGTTGATACCATGTCTTGATAGAAACCTTATCTACCAACTGCGATTGAAGCCGAATTTGACGTTTAGGGAGCACTACGAGCGGCATTGCCCGGCTCCAGAGCGGCGTTATAATTGCTTGATACCGCCCCCGCTTGGTTATAAGATACCTATAAGATGGCCGGAAAGTAGAGATGAAGTGTGGAAGGCCAACATTCCCCACACCCATCTTGCACAAGTGAAATCTGATCAGAATTGGATGGTTGTGAATGGGGACAAGATCAGTTTTCCGGGTGGTGGGACGCATTTCCACGATGGAGCTGATAAGTACATTGTTGCTCTTTCCAGGATGCTTAAGTTTCCTGGTGATAAATTCAACAATGGAGGCAATATCCGGAATGTTCTCGATGTGGGCTGTGGAGTTGCGAGTTTTGGGGCATATCTTCTTTCGCACGACGTCATAGCCCTGTCTCTTGCTCCGAATGATGCACATGAGAATCAGATACAATTTGCTCTAGAGAGGGGGATTCCATCCACTCTTGGTATCTTGGGTATAAAAAGACTTACTTATCCAAGCGGATCATTTGAGCTGGCTCATTGCTCACGCTGTCGAATAGATTGGCTTCAAAGAGATGGAATCCTGTTATTAGAGCTTGATAGATTACTGAGACCCGGAGGGTATTTCGTGTATACTTCTCCTGAAGCATATGCACTTGATCCAGAAAATAGGAGGATCTGGAGTGCTATGTCTGATCTTCTGAAAAGAATGTGCTGGAGAGTTGTAGCGAAGAAAGAACCGAGTGTTGTATGGGCCAAGCCCCTGACCAATAGTTGTTACTTGAAGAGAGATTCTAGGACCCAGCCCCCTCTGTGTGATTCCAAGGATGACCCAGATGCAAGTTGGAACGTGAGCATGAAGGCGTGCATTACTCGATACTCTGGAAAGATGCACAAGGAAAGAGGAAGTGGACTAGCTCCTTGGCCACAAAGGCTTACAGCAGCACCACCTCGCCTGGAAGAAATTGGCGTCAGTCCTGAAGAATTCCATGAGGACACTAACATTTGGCGCTTTAGAGTGATTGAGTACTGGAAGCAGATGAAGTCTGTCATACAGGAAAATTCAATTAGAAATGTCATGGATATGAACTCATACCTTGGTGGATTTGCTGCTGCCCTGAATGAAAAAGACGTCTGGGTAATGAATGTAGCTCCTGTCAATGTATCTGCCAGACTAAAGATCATCTACGATCGAGGATTAGTTGGAACTGTTCATGACTGGTGTGAAGCATTTTCGACATATCCACGTACATATGATCTTCTACATGCGTGGGAAGTATTCTCAGAGATTGCTGAACGGGGTTGCAATGCAGAAGATCTATTAATTGAAATGGATAGGATACTCAGACCAGATGGCTTGGTCATTATACGAGACAAACCTGCTGTTATAAACTATATTCGAAAGTTTCTGACTGCCTTGAAGTGGGATGGCTGGTTATCAGAAGTAGAACCGATGGTCGATGCTCTTTCCTCAAGCGACGAAAGAGTTTTGATTGCAAGAAAGAAGTTGTGGGACGAGGGGATTTCAGTAATATGTGCTAGATGCGGTATTGGTGCACGGCCTTGCCAGTCGAAGAGAATGTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009505 GO:0012505 GO:0016020 GO:0016740 GO:0016741 GO:0030312 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

635

Amino Acids

72.1

Weight (kDa)

7.52

Isoelectric Point (pI)

45.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	95 - 607	2.9e-221	Putative S-adenosyl-L-methionine-dependent methyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010488)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14430 AT5G14430
fragaria_vesca	FvH4_6g51950
malus_domestica	MD09G1021500.v1.1 MD17G1021500.v1.1
prunus_persica	Prupe.3G294800_v2.0.a1 Prupe.3G294800_v2.0.a1 Prupe.3G294800_v2.0.a1
pyrus_communis	pycom111g01790 pycom17g01860
rosa_chinensis	RchiOBHm_Chr2g0173431
rosa_laevigata	RLG00000022189
rosa_multiflora	Rmu_ssc0000282.1_g000015
rosa_roxburghii	Rroxscaffold_2G00078860
rosa_rugosa	Rorug02G0568600 Rorug02G0568700
rosa_samantha	Rh2AG648800 Rh2BG659400 Rh2CG624700 Rh2DG674600
rosa_wichuraiana	Rw2G053260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	393, 423, 1706
AatII	GACGTC	2 cut(s)	720, 1452
Acc36I	ACCTGC	1 cut(s)	1705
AccBSI	CCGCTC	2 cut(s)	364, 385
AccI	GTMKAC	1 cut(s)	950
AccII	CGCG	2 cut(s)	130, 201
AccIII	TCCGGA	1 cut(s)	654
AciI	CCGC	9 cut(s)	33, 130, 173, 364, 385, 407, 413, 837, 1863
AclWI	GGATC	5 cut(s)	129, 238, 847, 971, 1001
AcoI	YGGCCR	3 cut(s)	13, 440, 1241
AcsI	RAATTY	4 cut(s)	337, 632, 1301, 1378
AcuI	CTGAAG	3 cut(s)	71, 981, 1317
AcyI	GRCGYC	3 cut(s)	717, 1287, 1449
AfaI	GTAC	4 cut(s)	101, 590, 1346, 1567
AfiI	CCNNNNNNNGG	4 cut(s)	179, 416, 557, 1740
AflII	CTTAAG	1 cut(s)	614
AgsI	TTSAA	7 cut(s)	331, 637, 887, 1102, 1383, 1646, 1738
AjnI	CCWGG	4 cut(s)	81, 605, 622, 1272
AluBI	AGCT	9 cut(s)	19, 46, 149, 255, 581, 850, 913, 1235, 1469
AluI	AGCT	9 cut(s)	19, 46, 149, 255, 581, 850, 913, 1235, 1469
Alw21I	GWGCWC	3 cut(s)	234, 357, 1876
Alw26I	GTCTC	3 cut(s)	735, 922, 1683
Alw44I	GTGCAC	1 cut(s)	1872
AlwI	GGATC	5 cut(s)	129, 238, 847, 971, 1001
Aor13HI	TCCGGA	1 cut(s)	654
AoxI	GGCC	6 cut(s)	13, 440, 468, 1071, 1241, 1877
ApaLI	GTGCAC	1 cut(s)	1872
ApeKI	GCWGC	5 cut(s)	35, 46, 1259, 1427, 1430
ApoI	RAATTY	4 cut(s)	337, 632, 1301, 1378
AseI	ATTAAT	1 cut(s)	1640
AspLEI	GCGC	1 cut(s)	1329
AspS9I	GGNCC	2 cut(s)	1071, 1116
AsuC2I	CCSGG	4 cut(s)	123, 374, 552, 933
AsuHPI	GGTGA	1 cut(s)	638
AsuII	TTCGAA	1 cut(s)	1717
AvaII	GGWCC	1 cut(s)	1116
BaeGI	GKGCMC	1 cut(s)	1876
BalI	TGGCCA	2 cut(s)	15, 1243
BanII	GRGCYC	1 cut(s)	128
Bbv12I	GWGCWC	3 cut(s)	234, 357, 1876
BbvI	GCAGC	5 cut(s)	22, 33, 1271, 1414, 1417
BceAI	ACGGC	1 cut(s)	1892
BcgI	CGANNNNNNTGC	2 cut(s)	28, 62
BciT130I	CCWGG	4 cut(s)	83, 607, 624, 1274
BciVI	GTATCC	1 cut(s)	1650
BclI	TGATCA	1 cut(s)	508
BcnI	CCSGG	4 cut(s)	123, 374, 552, 933
BcoDI	GTCTC	3 cut(s)	735, 922, 1683
BfaI	CTAG	4 cut(s)	776, 1113, 1232, 1857
BfoI	RGCGCY	1 cut(s)	1330
BfrI	CTTAAG	1 cut(s)	614
BfuAI	ACCTGC	1 cut(s)	1705
BfuI	GTATCC	1 cut(s)	1650
BglI	GCCNNNNNGGC	1 cut(s)	1249
BglII	AGATCT	1 cut(s)	1633
BisI	GCNGC	7 cut(s)	36, 47, 365, 386, 1260, 1428, 1431
BlsI	GCNGC	7 cut(s)	37, 48, 366, 387, 1261, 1429, 1432
BmcAI	AGTACT	1 cut(s)	1346
Bme1390I	CCNGG	8 cut(s)	83, 123, 374, 552, 607, 624, 933, 1274
Bme18I	GGWCC	1 cut(s)	1116
BmgT120I	GGNCC	2 cut(s)	1071, 1116
BmiI	GGNNCC	3 cut(s)	157, 378, 1118
BmrFI	CCNGG	8 cut(s)	83, 123, 374, 552, 607, 624, 933, 1274
BmsI	GCATC	6 cut(s)	600, 736, 1144, 1197, 1768, 1850
BplI	GAGNNNNNCTC	2 cut(s)	221, 253
BpmI	CTGGAG	3 cut(s)	363, 1018, 1054
Bpu14I	TTCGAA	1 cut(s)	1717
BpuEI	CTTGAG	1 cut(s)	1774
BpuMI	CCSGG	4 cut(s)	123, 374, 552, 933
BsaAI	YACGTR	1 cut(s)	1565
BsaHI	GRCGYC	3 cut(s)	717, 1287, 1449
BsaI	GGTCTC	1 cut(s)	922
BsaJI	CCNNGG	4 cut(s)	82, 1140, 1238, 1414
BsaWI	WCCGGW	1 cut(s)	654
Bsc4I	CCNNNNNNNGG	4 cut(s)	179, 416, 557, 1740
Bse1I	ACTGG	3 cut(s)	1352, 1541, 1885
Bse3DI	GCAATG	4 cut(s)	72, 367, 856, 1630
BseAI	TCCGGA	1 cut(s)	654
BseBI	CCWGG	4 cut(s)	83, 607, 624, 1274
BseDI	CCNNGG	4 cut(s)	82, 1140, 1238, 1414
BseGI	GGATG	6 cut(s)	214, 525, 615, 791, 1150, 1750
BseLI	CCNNNNNNNGG	4 cut(s)	179, 416, 557, 1740
BseMI	GCAATG	4 cut(s)	72, 367, 856, 1630
BseMII	CTCAG	5 cut(s)	34, 231, 917, 1614, 1675
BseNI	ACTGG	3 cut(s)	1352, 1541, 1885
BseSI	GKGCMC	1 cut(s)	1876
BseXI	GCAGC	5 cut(s)	22, 33, 1271, 1414, 1417
BseYI	CCCAGC	1 cut(s)	1119
Bsh1236I	CGCG	2 cut(s)	130, 201
Bsh1285I	CGRYCG	1 cut(s)	1510
BshFI	GGCC	6 cut(s)	15, 442, 470, 1073, 1243, 1879
BsiEI	CGRYCG	1 cut(s)	1510
BsiHKAI	GWGCWC	3 cut(s)	234, 357, 1876
BsiSI	CCGG	7 cut(s)	122, 153, 374, 443, 551, 655, 933
BslFI	GGGAC	3 cut(s)	548, 574, 1844
BslI	CCNNNNNNNGG	4 cut(s)	179, 416, 557, 1740
BsmAI	GTCTC	3 cut(s)	735, 922, 1683
BsmFI	GGGAC	3 cut(s)	548, 574, 1844
BsnI	GGCC	6 cut(s)	15, 442, 470, 1073, 1243, 1879
Bso31I	GGTCTC	1 cut(s)	922
Bsp119I	TTCGAA	1 cut(s)	1717
Bsp1286I	GDGCHC	4 cut(s)	128, 234, 357, 1876
Bsp13I	TCCGGA	1 cut(s)	654
Bsp68I	TCGCGA	1 cut(s)	201
BspACI	CCGC	9 cut(s)	33, 130, 173, 364, 385, 407, 413, 837, 1863
BspANI	GGCC	6 cut(s)	15, 442, 470, 1073, 1243, 1879
BspCNI	CTCAG	5 cut(s)	33, 232, 918, 1613, 1674
BspEI	TCCGGA	1 cut(s)	654
BspFNI	CGCG	2 cut(s)	130, 201
BspHI	TCATGA	1 cut(s)	1531
BspLI	GGNNCC	3 cut(s)	157, 378, 1118
BspMI	ACCTGC	1 cut(s)	1705
BspPI	GGATC	5 cut(s)	129, 238, 847, 971, 1001
BspT104I	TTCGAA	1 cut(s)	1717
BspTI	CTTAAG	1 cut(s)	614
BspTNI	GGTCTC	1 cut(s)	922
BsrBI	CCGCTC	2 cut(s)	364, 385
BsrDI	GCAATG	4 cut(s)	72, 367, 856, 1630
BsrI	ACTGG	3 cut(s)	1352, 1541, 1885
BssECI	CCNNGG	4 cut(s)	82, 1140, 1238, 1414
BssNAI	GTATAC	1 cut(s)	951
BssNI	GRCGYC	3 cut(s)	717, 1287, 1449
BssT1I	CCWWGG	3 cut(s)	1140, 1238, 1414
Bst1107I	GTATAC	1 cut(s)	951
Bst2UI	CCWGG	4 cut(s)	83, 607, 624, 1274
Bst4CI	ACNGT	2 cut(s)	276, 1528
Bst6I	CTCTTC	2 cut(s)	1097, 1886
BstACI	GRCGYC	3 cut(s)	717, 1287, 1449
BstAFI	CTTAAG	1 cut(s)	614
BstBAI	YACGTR	1 cut(s)	1565
BstBI	TTCGAA	1 cut(s)	1717
BstC8I	GCNNGC	7 cut(s)	17, 79, 89, 128, 257, 852, 1183
BstDEI	CTNAG	5 cut(s)	20, 240, 926, 1600, 1661
BstF5I	GGATG	6 cut(s)	214, 525, 615, 791, 1150, 1750
BstFNI	CGCG	2 cut(s)	130, 201
BstH2I	RGCGCY	1 cut(s)	1330
BstHHI	GCGC	1 cut(s)	1329
BstMAI	GTCTC	3 cut(s)	735, 922, 1683
BstMCI	CGRYCG	1 cut(s)	1510
BstMWI	GCNNNNNNNGC	4 cut(s)	127, 331, 361, 1249
BstNI	CCWGG	4 cut(s)	83, 607, 624, 1274
BstNSI	RCATGY	1 cut(s)	1586
BstSCI	CCNGG	8 cut(s)	81, 121, 372, 550, 605, 622, 931, 1272
BstSLI	GKGCMC	1 cut(s)	1876
BstUI	CGCG	2 cut(s)	130, 201
BstV1I	GCAGC	5 cut(s)	22, 33, 1271, 1414, 1417
BstX2I	RGATCY	4 cut(s)	134, 243, 993, 1633
BstYI	RGATCY	4 cut(s)	134, 243, 993, 1633
BstZ17I	GTATAC	1 cut(s)	951
BsuI	GTATCC	1 cut(s)	1650
BsuRI	GGCC	6 cut(s)	15, 442, 470, 1073, 1243, 1879
BtgZI	GCGATG	1 cut(s)	182
BtsCI	GGATG	6 cut(s)	214, 525, 615, 791, 1150, 1750
BtuMI	TCGCGA	1 cut(s)	201
BveI	ACCTGC	1 cut(s)	1705
Cac8I	GCNNGC	7 cut(s)	17, 79, 89, 128, 257, 852, 1183
CciI	TCATGA	1 cut(s)	1531
CfoI	GCGC	1 cut(s)	1329
Cfr13I	GGNCC	2 cut(s)	1071, 1116
CseI	GACGC	2 cut(s)	571, 1276
Csp6I	GTAC	4 cut(s)	100, 589, 1345, 1566
CviAII	CATG	8 cut(s)	218, 294, 752, 1174, 1307, 1396, 1532, 1583
CviQI	GTAC	4 cut(s)	100, 589, 1345, 1566
DdeI	CTNAG	5 cut(s)	20, 240, 926, 1600, 1661
EaeI	YGGCCR	3 cut(s)	13, 440, 1241
Eam1104I	CTCTTC	2 cut(s)	1097, 1886
EarI	CTCTTC	2 cut(s)	1097, 1886
Eco130I	CCWWGG	3 cut(s)	1140, 1238, 1414
Eco24I	GRGCYC	1 cut(s)	128
Eco31I	GGTCTC	1 cut(s)	922
Eco47I	GGWCC	1 cut(s)	1116
Eco57I	CTGAAG	3 cut(s)	71, 981, 1317
EcoO109I	RGGNCCY	1 cut(s)	1116
EcoRI	GAATTC	1 cut(s)	1301
EcoRII	CCWGG	4 cut(s)	81, 605, 622, 1272
EcoT14I	CCWWGG	3 cut(s)	1140, 1238, 1414
EcoT38I	GRGCYC	1 cut(s)	128
ErhI	CCWWGG	3 cut(s)	1140, 1238, 1414
FaeI	CATG	8 cut(s)	221, 297, 755, 1177, 1310, 1399, 1535, 1586
FalI	AAGNNNNNCTT	2 cut(s)	811, 843
FaqI	GGGAC	3 cut(s)	548, 574, 1844
FatI	CATG	8 cut(s)	217, 293, 751, 1173, 1306, 1395, 1531, 1582
FauI	CCCGC	3 cut(s)	40, 180, 420
FauNDI	CATATG	2 cut(s)	967, 1570
FbaI	TGATCA	1 cut(s)	508
FblI	GTMKAC	1 cut(s)	950
Fnu4HI	GCNGC	7 cut(s)	36, 47, 365, 386, 1260, 1428, 1431
FokI	GGATG	6 cut(s)	221, 532, 622, 778, 1157, 1757
FriOI	GRGCYC	1 cut(s)	128
Fsp4HI	GCNGC	7 cut(s)	36, 47, 365, 386, 1260, 1428, 1431
FspBI	CTAG	4 cut(s)	776, 1113, 1232, 1857
GlaI	GCGC	1 cut(s)	1328
GluI	GCNGC	7 cut(s)	36, 47, 365, 386, 1260, 1428, 1431
GsaI	CCCAGC	1 cut(s)	1123
GsuI	CTGGAG	3 cut(s)	363, 1018, 1054
HaeII	RGCGCY	1 cut(s)	1330
HaeIII	GGCC	6 cut(s)	15, 442, 470, 1073, 1243, 1879
HapII	CCGG	7 cut(s)	122, 153, 374, 443, 551, 655, 933
HgaI	GACGC	2 cut(s)	571, 1276
HhaI	GCGC	1 cut(s)	1329
Hin1I	GRCGYC	3 cut(s)	717, 1287, 1449
Hin1II	CATG	8 cut(s)	221, 297, 755, 1177, 1310, 1399, 1535, 1586
Hin6I	GCGC	1 cut(s)	1327
HinP1I	GCGC	1 cut(s)	1327
HindIII	AAGCTT	2 cut(s)	147, 253
HinfI	GANTC	6 cut(s)	165, 757, 787, 897, 1109, 1136
HpaII	CCGG	7 cut(s)	122, 153, 374, 443, 551, 655, 933
HphI	GGTGA	1 cut(s)	638
Hpy166II	GTNNAC	3 cut(s)	951, 1229, 1874
Hpy8I	GTNNAC	3 cut(s)	951, 1229, 1874
Hpy99I	CGWCG	2 cut(s)	719, 1799
HpyAV	CCTTC	2 cut(s)	460, 1171
HpyCH4III	ACNGT	2 cut(s)	276, 1528
HpyCH4IV	ACGT	5 cut(s)	344, 717, 1167, 1449, 1564
HpyF10VI	GCNNNNNNNGC	4 cut(s)	127, 331, 361, 1249
HpyF3I	CTNAG	5 cut(s)	20, 240, 926, 1600, 1661
HpySE526I	ACGT	5 cut(s)	344, 717, 1167, 1449, 1564
Hsp92I	GRCGYC	3 cut(s)	717, 1287, 1449
Hsp92II	CATG	8 cut(s)	221, 297, 755, 1177, 1310, 1399, 1535, 1586
HspAI	GCGC	1 cut(s)	1327
Kpn2I	TCCGGA	1 cut(s)	654
Ksp22I	TGATCA	1 cut(s)	508
LmnI	GCTCC	8 cut(s)	161, 229, 352, 382, 578, 742, 1240, 1474
Lsp1109I	GCAGC	5 cut(s)	22, 33, 1271, 1414, 1417
LweI	GCATC	6 cut(s)	600, 736, 1144, 1197, 1768, 1850
MaeI	CTAG	4 cut(s)	776, 1113, 1232, 1857
MaeII	ACGT	5 cut(s)	344, 717, 1167, 1449, 1564
MaeIII	GTNAC	1 cut(s)	1094
MbiI	CCGCTC	2 cut(s)	364, 385
MflI	RGATCY	4 cut(s)	134, 243, 993, 1633
MhlI	GDGCHC	4 cut(s)	128, 234, 357, 1876
MlsI	TGGCCA	2 cut(s)	15, 1243
MluNI	TGGCCA	2 cut(s)	15, 1243
MlyI	GAGTC	1 cut(s)	159
MmeI	TCCRAC	2 cut(s)	1142, 1501
Mox20I	TGGCCA	2 cut(s)	15, 1243
MroI	TCCGGA	1 cut(s)	654
MscI	TGGCCA	2 cut(s)	15, 1243
MseI	TTAA	3 cut(s)	249, 615, 1640
MslI	CAYNNNNRTG	1 cut(s)	1400
Msp20I	TGGCCA	2 cut(s)	15, 1243
MspA1I	CMGCKG	1 cut(s)	35
MspCI	CTTAAG	1 cut(s)	614
MspI	CCGG	7 cut(s)	122, 153, 374, 443, 551, 655, 933
MspR9I	CCNGG	8 cut(s)	83, 123, 374, 552, 607, 624, 933, 1274
MvaI	CCWGG	4 cut(s)	83, 607, 624, 1274
MvnI	CGCG	2 cut(s)	130, 201
MwoI	GCNNNNNNNGC	4 cut(s)	127, 331, 361, 1249
NciI	CCSGG	4 cut(s)	123, 374, 552, 933
NdeI	CATATG	2 cut(s)	967, 1570
NlaIII	CATG	8 cut(s)	221, 297, 755, 1177, 1310, 1399, 1535, 1586
NlaIV	GGNNCC	3 cut(s)	157, 378, 1118
NruI	TCGCGA	1 cut(s)	201
NspI	RCATGY	1 cut(s)	1586
NspV	TTCGAA	1 cut(s)	1717
PagI	TCATGA	1 cut(s)	1531
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 186
PfeI	GAWTC	5 cut(s)	757, 787, 897, 1109, 1136
PfoI	TCCNGGA	1 cut(s)	605
PkrI	GCNGC	7 cut(s)	37, 48, 366, 387, 1261, 1429, 1432
Ple19I	CGATCG	1 cut(s)	1510
PleI	GAGTC	1 cut(s)	159
PpsI	GAGTC	1 cut(s)	159
Ppu21I	YACGTR	1 cut(s)	1565
PpuMI	RGGWCCY	1 cut(s)	1116
PshBI	ATTAAT	1 cut(s)	1640
PsiI	TTATAA	3 cut(s)	393, 423, 1706
Psp5II	RGGWCCY	1 cut(s)	1116
Psp6I	CCWGG	4 cut(s)	81, 605, 622, 1272
PspFI	CCCAGC	1 cut(s)	1119
PspGI	CCWGG	4 cut(s)	81, 605, 622, 1272
PspN4I	GGNNCC	3 cut(s)	157, 378, 1118
PspPI	GGNCC	2 cut(s)	1071, 1116
PspPPI	RGGWCCY	1 cut(s)	1116
PsuI	RGATCY	4 cut(s)	134, 243, 993, 1633
PvuI	CGATCG	1 cut(s)	1510
RruI	TCGCGA	1 cut(s)	201
RsaI	GTAC	4 cut(s)	101, 590, 1346, 1567
RsaNI	GTAC	4 cut(s)	100, 589, 1345, 1566
RseI	CAYNNNNRTG	1 cut(s)	1400
SaqAI	TTAA	3 cut(s)	249, 615, 1640
SatI	GCNGC	7 cut(s)	36, 47, 365, 386, 1260, 1428, 1431
Sau96I	GGNCC	2 cut(s)	1071, 1116
ScaI	AGTACT	1 cut(s)	1346
SchI	GAGTC	1 cut(s)	159
ScrFI	CCNGG	8 cut(s)	83, 123, 374, 552, 607, 624, 933, 1274
SduI	GDGCHC	4 cut(s)	128, 234, 357, 1876
SfaNI	GCATC	6 cut(s)	600, 736, 1144, 1197, 1768, 1850
SfuI	TTCGAA	1 cut(s)	1717
SinI	GGWCC	1 cut(s)	1116
SmiMI	CAYNNNNRTG	1 cut(s)	1400
SmlI	CTYRAG	2 cut(s)	614, 1789
SmoI	CTYRAG	2 cut(s)	614, 1789
SsiI	CCGC	9 cut(s)	33, 130, 173, 364, 385, 407, 413, 837, 1863
SspMI	CTAG	4 cut(s)	776, 1113, 1232, 1857
StyD4I	CCNGG	8 cut(s)	81, 121, 372, 550, 605, 622, 931, 1272
StyI	CCWWGG	3 cut(s)	1140, 1238, 1414
TaaI	ACNGT	2 cut(s)	276, 1528
TaiI	ACGT	5 cut(s)	347, 720, 1170, 1452, 1567
TaqI	TCGA	8 cut(s)	666, 871, 1192, 1510, 1553, 1717, 1776, 1889
TatI	WGTACW	3 cut(s)	99, 588, 1344
TauI	GCSGC	2 cut(s)	367, 388
TfiI	GAWTC	5 cut(s)	757, 787, 897, 1109, 1136
Tru1I	TTAA	3 cut(s)	249, 615, 1640
Tru9I	TTAA	3 cut(s)	249, 615, 1640
TseI	GCWGC	5 cut(s)	35, 46, 1259, 1427, 1430
TspDTI	ATGAA	9 cut(s)	17, 156, 471, 1190, 1373, 1418, 1455, 1475, 1520
Vha464I	CTTAAG	1 cut(s)	614
VneI	GTGCAC	1 cut(s)	1872
VpaK11BI	GGWCC	1 cut(s)	1116
VspI	ATTAAT	1 cut(s)	1640
XapI	RAATTY	4 cut(s)	337, 632, 1301, 1378
XbaI	TCTAGA	1 cut(s)	775
XceI	RCATGY	1 cut(s)	1586
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1158
XmiI	GTMKAC	1 cut(s)	950
XspI	CTAG	4 cut(s)	776, 1113, 1232, 1857
ZraI	GACGTC	2 cut(s)	718, 1450
ZrmI	AGTACT	1 cut(s)	1346

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.