Rroxscaffold_2G00078860

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010488
Physical Location & Seq
Forward (+)
2032420 .. 2035538
3119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078860.1

Sequence Viewer

Length: 1584 bp
ATGCAATTCTCGGAGTTGATACCTTGTCTCGATAGAAACCTCATTTACCAGCTGAAATTGAAGGTAAATTTGACACTTATGGAGCATTATGAGCGGCATTGTCCCCCGCCGGAGCGGCGGTACAATTGCTTGATCCCACCCCCAGTAGGATACAAGGTCCCCGTGAGATGGCCGGCGAGTAGAGATGAAGTGTGGAAGGCCAACATCCCCCATACGTCTCTTGCAGAAGAGAAATCGGATCAGAATTGGATGGTGGTGAATGGTGACAAGATCAATTTCCCCGGCGGAGGGACACATTTCCATACTGGAGCTGATAAGTACATTGTTGCTCTTTCCAAGATGCTTAAGTTTCCTGATTTTAAGTTCAACAACGGTGGAAATATTCGCAATGTTCTTGATGTGGGTTGTGGAGTTGCAAGTTTTGGGGCGTATCTTCTTTCTCATCATGTCATTGCCATGTCCCTTGCTCCTAATGATGTGCATGAGAATCAAATACAATTTGCATTAGAGAGGGGGATTCCAGCAACTCTTGGTATCTTGGGTACAAAAAGACTTACTTATCCTAGCAGATCATTCGAGCTGGCTCATTGCTCACGATGCCGGATAGATTGGCTTCAAAGAGATGGAATCCTCTTATTAGAGCTTGACAGGTTACTAAGACCTGGAGGGTATTTTGTGTACACTTCTCCTGAAGCATATGCTCATGACCCTGAAAATCAAAAGATCTGGAATGCTATGTCTGATCTTTCGAAAAGAATGTGCTGGAAAGTTGCAGCCAAGAAAGAGCAGAGTGTTGTATGGGCCAAGCCACTGACTAATAGTTGTTACTTGAAGAGAGATCCTGGGACCCGACCCCCTCTGTGCAATTCTGATGATGACCCAGATGCAATTTGGAATGTGAGCATGAAGACGTGCATTTCTCAATATTCTGCAAAGGTGCATAGGGATAGAGGAAGCAGACTTGCCCCTTGGCCTCAGAGGCTTACGACACCACCCCCTCGCCTGGAGGAAATTGGTGTCAGTCCTGAAGAATTCCAAGAGGACACTAGCATATGGCATTTTAGAGTCATTGAATACTGGAAGCAGATGAAGTCTGTCATACAGAGAAATTCATTCAGAAATGTCATGGATATGAATTCACAGCTTGGTGGATTTGCTACTGCCTTGAATGGAAAAGATGTCTGGGTTATGAATGTTGCTCCTGTCCATGTATCTGCCAAATTAAAGATTGTTTATGATCGGGGCTTGATTGGAACTGTTCATGACTGGTGTGAAGCGTTTTCCACATACCCACGTACATATGATCTTCTACATGCATGGGCAGTATTTTCAGATATTGAGGAACGTGGATGCAGTGCAGAGGATCTATTGATTGAAATGGATAGGATACTGAGACCCAATGGGTTTGTCATCATAAGAGACAAACCCTCTGTTATAAGCTATATCCGAAAGTTTGTGACTGCCTTGAAGTGGGATGACTGGTTATCAGAAGTCGAACCTCGAGTTGATGCTCTTTCCTCAGGTGAAGAGAGAGTTTTGATTGCAAGAAAGAAGTTGTGGGATGACGAGCATACGACAATGTGA

Protein Analysis

527

Amino Acids

60.5

Weight (kDa)

7.92

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 5 - 517 8.3e-224 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1436
AccBSI CCGCTC 2 cut(s) 94, 115
AciI CCGC 5 cut(s) 94, 107, 115, 118, 285
AclWI GGATC 4 cut(s) 127, 246, 833, 1371
AcoI YGGCCR 1 cut(s) 170
AcsI RAATTY 4 cut(s) 67, 1031, 1108, 1135
AcuI CTGAAG 2 cut(s) 711, 1047
AfaI GTAC 5 cut(s) 122, 320, 544, 680, 1297
AfiI CCNNNNNNNGG 6 cut(s) 146, 168, 287, 288, 1003, 1470
AflII CTTAAG 1 cut(s) 344
AgsI TTSAA 8 cut(s) 61, 367, 617, 832, 1073, 1168, 1376, 1468
AjiI CACGTC 1 cut(s) 912
AjnI CCWGG 3 cut(s) 661, 841, 1002
AluBI AGCT 6 cut(s) 52, 311, 580, 643, 1144, 1440
AluI AGCT 6 cut(s) 52, 311, 580, 643, 1144, 1440
Alw26I GTCTC 4 cut(s) 32, 222, 1387, 1413
AlwI GGATC 4 cut(s) 127, 246, 833, 1371
Ama87I CYCGRG 1 cut(s) 1500
AoxI GGCC 4 cut(s) 170, 198, 801, 971
ApeKI GCWGC 1 cut(s) 773
ApoI RAATTY 4 cut(s) 67, 1031, 1108, 1135
AspS9I GGNCC 3 cut(s) 157, 801, 846
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 3 cut(s) 268, 275, 1535
AsuII TTCGAA 1 cut(s) 749
AvaI CYCGRG 1 cut(s) 1500
AvaII GGWCC 2 cut(s) 157, 846
AxyI CCTNAGG 1 cut(s) 1519
BbsI GAAGAC 1 cut(s) 914
BbvI GCAGC 1 cut(s) 785
BccI CCATC 3 cut(s) 162, 244, 617
BcgI CGANNNNNNTGC 2 cut(s) 556, 590
BciT130I CCWGG 3 cut(s) 663, 843, 1004
BciVI GTATCC 2 cut(s) 143, 1380
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 4 cut(s) 32, 222, 1387, 1413
BfaI CTAG 2 cut(s) 564, 1047
BfrI CTTAAG 1 cut(s) 344
BfuI GTATCC 2 cut(s) 143, 1380
BglI GCCNNNNNGGC 2 cut(s) 115, 979
BglII AGATCT 1 cut(s) 723
BisI GCNGC 3 cut(s) 95, 116, 774
BlsI GCNGC 3 cut(s) 96, 117, 775
Bme1390I CCNGG 4 cut(s) 282, 663, 843, 1004
Bme18I GGWCC 2 cut(s) 157, 846
BmeT110I CYCGRG 1 cut(s) 1500
BmgBI CACGTC 1 cut(s) 912
BmgT120I GGNCC 3 cut(s) 157, 801, 846
BmiI GGNNCC 3 cut(s) 159, 847, 848
BmrFI CCNGG 4 cut(s) 282, 663, 843, 1004
BmrI ACTGGG 1 cut(s) 137
BmsI GCATC 5 cut(s) 330, 587, 874, 1340, 1498
BmuI ACTGGG 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 914
BpmI CTGGAG 3 cut(s) 327, 684, 1025
Bpu14I TTCGAA 1 cut(s) 749
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 1295
BsaI GGTCTC 1 cut(s) 1387
BsaJI CCNNGG 3 cut(s) 280, 842, 968
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 6 cut(s) 146, 168, 287, 288, 1003, 1470
Bse118I RCCGGY 1 cut(s) 172
Bse1I ACTGG 5 cut(s) 143, 310, 1082, 1271, 1484
Bse21I CCTNAGG 1 cut(s) 1519
Bse3DI GCAATG 3 cut(s) 394, 450, 586
BseBI CCWGG 3 cut(s) 663, 843, 1004
BseDI CCNNGG 3 cut(s) 280, 842, 968
BseGI GGATG 5 cut(s) 204, 255, 1355, 1480, 1567
BseLI CCNNNNNNNGG 6 cut(s) 146, 168, 287, 288, 1003, 1470
BseMI GCAATG 3 cut(s) 394, 450, 586
BseMII CTCAG 3 cut(s) 989, 1382, 1533
BseNI ACTGG 5 cut(s) 143, 310, 1082, 1271, 1484
BseXI GCAGC 1 cut(s) 785
BsgI GTGCAG 1 cut(s) 1377
BshFI GGCC 4 cut(s) 172, 200, 803, 973
BsiHKCI CYCGRG 1 cut(s) 1500
BsiSI CCGG 4 cut(s) 110, 173, 282, 601
BslFI GGGAC 5 cut(s) 87, 143, 304, 445, 859
BslI CCNNNNNNNGG 6 cut(s) 146, 168, 287, 288, 1003, 1470
BsmAI GTCTC 4 cut(s) 32, 222, 1387, 1413
BsmBI CGTCTC 1 cut(s) 222
BsmFI GGGAC 5 cut(s) 87, 143, 304, 445, 859
BsmI GAATGC 1 cut(s) 736
BsnI GGCC 4 cut(s) 172, 200, 803, 973
Bso31I GGTCTC 1 cut(s) 1387
BsoBI CYCGRG 1 cut(s) 1500
Bsp119I TTCGAA 1 cut(s) 749
Bsp1407I TGTACA 1 cut(s) 678
BspACI CCGC 5 cut(s) 94, 107, 115, 118, 285
BspANI GGCC 4 cut(s) 172, 200, 803, 973
BspCNI CTCAG 3 cut(s) 988, 1383, 1532
BspHI TCATGA 2 cut(s) 703, 1261
BspLI GGNNCC 3 cut(s) 159, 847, 848
BspPI GGATC 4 cut(s) 127, 246, 833, 1371
BspT104I TTCGAA 1 cut(s) 749
BspTI CTTAAG 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 1387
BsrBI CCGCTC 2 cut(s) 94, 115
BsrDI GCAATG 3 cut(s) 394, 450, 586
BsrFI RCCGGY 1 cut(s) 172
BsrGI TGTACA 1 cut(s) 678
BsrI ACTGG 5 cut(s) 143, 310, 1082, 1271, 1484
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 280, 842, 968
BssT1I CCWWGG 1 cut(s) 968
Bst2UI CCWGG 3 cut(s) 663, 843, 1004
Bst4CI ACNGT 2 cut(s) 374, 1258
Bst6I CTCTTC 3 cut(s) 222, 827, 1521
BstAFI CTTAAG 1 cut(s) 344
BstAUI TGTACA 1 cut(s) 678
BstBAI YACGTR 1 cut(s) 1295
BstBI TTCGAA 1 cut(s) 749
BstC8I GCNNGC 2 cut(s) 174, 582
BstDEI CTNAG 4 cut(s) 656, 975, 1391, 1519
BstF5I GGATG 5 cut(s) 204, 255, 1355, 1480, 1567
BstMAI GTCTC 4 cut(s) 32, 222, 1387, 1413
BstMWI GCNNNNNNNGC 4 cut(s) 91, 115, 597, 979
BstNI CCWGG 3 cut(s) 663, 843, 1004
BstNSI RCATGY 1 cut(s) 1316
BstSCI CCNGG 4 cut(s) 280, 661, 841, 1002
BstV1I GCAGC 1 cut(s) 785
BstV2I GAAGAC 1 cut(s) 914
BstX2I RGATCY 3 cut(s) 723, 838, 1363
BstYI RGATCY 3 cut(s) 723, 838, 1363
Bsu36I CCTNAGG 1 cut(s) 1519
BsuI GTATCC 2 cut(s) 143, 1380
BsuRI GGCC 4 cut(s) 172, 200, 803, 973
BtrI CACGTC 1 cut(s) 912
BtsCI GGATG 5 cut(s) 204, 255, 1355, 1480, 1567
BtsI GCAGTG 1 cut(s) 1360
BtsIMutI CAGTG 2 cut(s) 809, 1360
Cac8I GCNNGC 2 cut(s) 174, 582
CciI TCATGA 2 cut(s) 703, 1261
Cfr10I RCCGGY 1 cut(s) 172
Cfr13I GGNCC 3 cut(s) 157, 801, 846
Csp6I GTAC 5 cut(s) 121, 319, 543, 679, 1296
CspCI CAANNNNNGTGG 2 cut(s) 355, 390
CviQI GTAC 5 cut(s) 121, 319, 543, 679, 1296
DdeI CTNAG 4 cut(s) 656, 975, 1391, 1519
EaeI YGGCCR 1 cut(s) 170
Eam1104I CTCTTC 3 cut(s) 222, 827, 1521
EarI CTCTTC 3 cut(s) 222, 827, 1521
EciI GGCGGA 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 968
Eco31I GGTCTC 1 cut(s) 1387
Eco47I GGWCC 2 cut(s) 157, 846
Eco57I CTGAAG 2 cut(s) 711, 1047
Eco81I CCTNAGG 1 cut(s) 1519
Eco88I CYCGRG 1 cut(s) 1500
EcoO109I RGGNCCY 2 cut(s) 157, 846
EcoRI GAATTC 2 cut(s) 1031, 1135
EcoRII CCWGG 3 cut(s) 661, 841, 1002
EcoT14I CCWWGG 1 cut(s) 968
EcoT22I ATGCAT 1 cut(s) 1318
ErhI CCWWGG 1 cut(s) 968
Esp3I CGTCTC 1 cut(s) 222
FalI AAGNNNNNCTT 2 cut(s) 541, 573
FaqI GGGAC 5 cut(s) 87, 143, 304, 445, 859
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 3 cut(s) 697, 1052, 1300
Fnu4HI GCNGC 3 cut(s) 95, 116, 774
FokI GGATG 5 cut(s) 191, 262, 1362, 1487, 1574
Fsp4HI GCNGC 3 cut(s) 95, 116, 774
FspBI CTAG 2 cut(s) 564, 1047
GluI GCNGC 3 cut(s) 95, 116, 774
GsuI CTGGAG 3 cut(s) 327, 684, 1025
HaeIII GGCC 4 cut(s) 172, 200, 803, 973
HapII CCGG 4 cut(s) 110, 173, 282, 601
HinfI GANTC 4 cut(s) 487, 517, 627, 1065
HpaII CCGG 4 cut(s) 110, 173, 282, 601
HphI GGTGA 3 cut(s) 268, 275, 1535
Hpy166II GTNNAC 2 cut(s) 679, 681
Hpy188III TCNNGA 9 cut(s) 29, 353, 395, 594, 689, 704, 727, 1025, 1262
Hpy8I GTNNAC 2 cut(s) 679, 681
HpyAV CCTTC 2 cut(s) 55, 190
HpyCH4III ACNGT 2 cut(s) 374, 1258
HpyCH4IV ACGT 4 cut(s) 215, 911, 1294, 1345
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 115, 597, 979
HpyF3I CTNAG 4 cut(s) 656, 975, 1391, 1519
HpySE526I ACGT 4 cut(s) 215, 911, 1294, 1345
KflI GGGWCCC 1 cut(s) 846
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
LmnI GCTCC 5 cut(s) 82, 112, 308, 472, 1204
Lsp1109I GCAGC 1 cut(s) 785
LweI GCATC 5 cut(s) 330, 587, 874, 1340, 1498
MaeI CTAG 2 cut(s) 564, 1047
MaeII ACGT 4 cut(s) 215, 911, 1294, 1345
MaeIII GTNAC 4 cut(s) 263, 651, 824, 1456
MbiI CCGCTC 2 cut(s) 94, 115
MboII GAAGA 7 cut(s) 239, 425, 844, 919, 1040, 1298, 1538
MfeI CAATTG 1 cut(s) 124
MflI RGATCY 3 cut(s) 723, 838, 1363
MlyI GAGTC 1 cut(s) 1074
Mph1103I ATGCAT 1 cut(s) 1318
MroNI GCCGGC 1 cut(s) 172
MseI TTAA 3 cut(s) 345, 360, 1223
MslI CAYNNNNRTG 2 cut(s) 455, 1130
MspA1I CMGCKG 1 cut(s) 52
MspCI CTTAAG 1 cut(s) 344
MspI CCGG 4 cut(s) 110, 173, 282, 601
MspR9I CCNGG 4 cut(s) 282, 663, 843, 1004
MunI CAATTG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 3 cut(s) 663, 843, 1004
MwoI GCNNNNNNNGC 4 cut(s) 91, 115, 597, 979
NaeI GCCGGC 1 cut(s) 174
NciI CCSGG 1 cut(s) 282
NdeI CATATG 3 cut(s) 697, 1052, 1300
NgoMIV GCCGGC 1 cut(s) 172
NlaIV GGNNCC 3 cut(s) 159, 847, 848
NmuCI GTSAC 2 cut(s) 263, 1456
NsiI ATGCAT 1 cut(s) 1318
NspI RCATGY 1 cut(s) 1316
NspV TTCGAA 1 cut(s) 749
PaeR7I CTCGAG 1 cut(s) 1500
PagI TCATGA 2 cut(s) 703, 1261
PctI GAATGC 1 cut(s) 736
PdiI GCCGGC 1 cut(s) 174
PfeI GAWTC 3 cut(s) 487, 517, 627
PkrI GCNGC 3 cut(s) 96, 117, 775
PleI GAGTC 1 cut(s) 1073
PpsI GAGTC 1 cut(s) 1073
Ppu21I YACGTR 1 cut(s) 1295
PpuMI RGGWCCY 2 cut(s) 157, 846
PsiI TTATAA 1 cut(s) 1436
Psp5II RGGWCCY 2 cut(s) 157, 846
Psp6I CCWGG 3 cut(s) 661, 841, 1002
PspGI CCWGG 3 cut(s) 661, 841, 1002
PspN4I GGNNCC 3 cut(s) 159, 847, 848
PspPI GGNCC 3 cut(s) 157, 801, 846
PspPPI RGGWCCY 2 cut(s) 157, 846
PspXI VCTCGAGB 1 cut(s) 1500
PsuI RGATCY 3 cut(s) 723, 838, 1363
PvuII CAGCTG 1 cut(s) 52
RsaI GTAC 5 cut(s) 122, 320, 544, 680, 1297
RsaNI GTAC 5 cut(s) 121, 319, 543, 679, 1296
RseI CAYNNNNRTG 2 cut(s) 455, 1130
SaqAI TTAA 3 cut(s) 345, 360, 1223
SatI GCNGC 3 cut(s) 95, 116, 774
Sau96I GGNCC 3 cut(s) 157, 801, 846
SchI GAGTC 1 cut(s) 1074
ScrFI CCNGG 4 cut(s) 282, 663, 843, 1004
SfaNI GCATC 5 cut(s) 330, 587, 874, 1340, 1498
Sfr274I CTCGAG 1 cut(s) 1500
SfuI TTCGAA 1 cut(s) 749
SinI GGWCC 2 cut(s) 157, 846
SlaI CTCGAG 1 cut(s) 1500
SmiMI CAYNNNNRTG 2 cut(s) 455, 1130
SmlI CTYRAG 2 cut(s) 344, 1500
SmoI CTYRAG 2 cut(s) 344, 1500
SsiI CCGC 5 cut(s) 94, 107, 115, 118, 285
SspI AATATT 2 cut(s) 382, 926
SspMI CTAG 2 cut(s) 564, 1047
StyD4I CCNGG 4 cut(s) 280, 661, 841, 1002
StyI CCWWGG 1 cut(s) 968
TaaI ACNGT 2 cut(s) 374, 1258
TaiI ACGT 4 cut(s) 218, 914, 1297, 1348
TaqI TCGA 5 cut(s) 30, 576, 749, 1494, 1501
TatI WGTACW 2 cut(s) 318, 678
TauI GCSGC 2 cut(s) 97, 118
TfiI GAWTC 3 cut(s) 487, 517, 627
Tru1I TTAA 3 cut(s) 345, 360, 1223
Tru9I TTAA 3 cut(s) 345, 360, 1223
TscAI CASTG 2 cut(s) 816, 1360
TseFI GTSAC 2 cut(s) 263, 1456
TseI GCWGC 1 cut(s) 773
Tsp45I GTSAC 2 cut(s) 263, 1456
TspDTI ATGAA 7 cut(s) 201, 920, 1101, 1103, 1148, 1205, 1250
TspRI CASTG 2 cut(s) 816, 1360
Vha464I CTTAAG 1 cut(s) 344
VpaK11BI GGWCC 2 cut(s) 157, 846
XapI RAATTY 4 cut(s) 67, 1031, 1108, 1135
XceI RCATGY 1 cut(s) 1316
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
XhoI CTCGAG 1 cut(s) 1500
XspI CTAG 2 cut(s) 564, 1047
Zsp2I ATGCAT 1 cut(s) 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.