Rh2CG624700

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0010488
Physical Location & Seq
Reverse (-)
79906259 .. 79908724
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG624700.1

Sequence Viewer

Length: 1794 bp
ATGAAGCACAGGAATGACGTCAGCTTCCCCAAGTTGCTCAAGTCCGTTCTGCTCGGCCTCACTGTGTGCCTGGGTCTCCTCTGTTTCTACTACGGCTGGTCCTTCGCTCCGGGCTCCCGCCGCTCCGACCACGAAGAATTCGACCCGGTTTTCGGTGGGTCCCTCACCCATCGTCGTCGCCGCGAGGCCGAGGCCGAGCCCCCCAAAAGCATTCCTATTTGTGATATGCAATTCTCGGAGTTGATACCTTGTCTCGATAGAAACCTCATTTACCAGCTGAAATTGAAGGTAAATTTGACACTTATGGAGCATTATGAACGGCATTGTCCCCCGCCGGAGCGGCGGTACAATTGCTTGATACCACCACCGTTAGGATACAAGGTCCCGGTGAGATGGCCTACGAGTAGAGATGAAGTGTGGAAGGCCAACATCCCCCATACGTCTCTTGCAGAAGAGAAATCGGATCAGAATTGGATGGTTGTGAATGGTGACAAGATCAATTTCCCCGGCGGAGGGACACATTTCCATACTGGAGCTGATAAGTACATTGTTGCTCTTTCCAAGATGCTTAAGTTTCCTGATTTTAAGTTCAACAACGGTGGAAATATTCGCAATGTTCTTGATGTGGGTTGTGGAGTTGCAAGTTTTGGGGCGTATCTTCTTTCTCATCATGTCATTGCCATGTCCCTTGCTCCTAATGATGTGCATGAGAATCAAATACAATTTGCATTAGAGAGGGGGATTCCAGCAACTCTTGGTATCTTGGGCACAAAAAGACTTACTTATCCTAGCAGATCATTTGAGCTGGCTCATTGCTCACGCTGCCGGATAGATTGGCTTCAAAGAGATGGAATCCTCTTATTAGAGCTTGACAGGTTACTAAGACCTGGAGGGTATTTTGTGTACACTTCTCCTGAAGCGTATGCTCATGATCCTGAAAATCAAAAGATCTGGAATGCTATGTCTGATCTTTCAAAAAGAATGTGCTGGAAAGTTGCAGCCAAGAAAGATCAGAGTGTTGTATGGGCCAAGCCACTGACTAATAGTTGTTACTTGAAGAGAGATCCTTGGACCCAACCCCCTCTGTGCAATTCTGTTGATGACCCAGATGCAGTTTGGAATGTAAGCATGAAGACGTGCATTTCTCAATATTCTGCAAAGGTGCATAGGGATAGAGGAAGCAGACTTGCCCCTTGGCCTCAGAGGCTTACGACACCACCCCCTCGCCTGGAGGAAATTGGTGTCAGTCCTGAAGAATTCCAAGAGGACACTAGCATATGGCATTTTAGAGTCATTGAATACTGGAAGCAGATGAAGTCTGTCATACAGAGAAATTCATTCAGAAATGTCATGGATATGAATTCACAGCTTGGTGGATTTGCTACTGCCTTGAATGGAAAAGATGTCTGGGTTATGAATGTTGCTCCTGTCCATGTATCTGCCAAATTAAAGATTGTTTATGATCGGGGCTTGATTGGAACTGTTCATGACTGGTGTGAAGCGTTTTCCACATACCCACGTACATATGATCTTCTACATGCATGGGCAGTATTTTCAGATATTGAGGAACGTGGATGCAGTGCAGAGGATCTATTGATTGAAATGGATAGGATACTGAGACCCAATGGGTTTGTCATCATAAGAGACAAACCCTCTGTTATAAACTATATCCGAAAGTTTGTAACTGCCTTGAAGTGGGATGACTGGTTATCAGAAGTTGAACCTCGAGTTGATGCTCTTTCCTCAGGTGAAGAGAGAGTTTTGATTGCAAGAAAGAAGTTGTGGGATGACTAG

Protein Analysis

597

Amino Acids

68.55

Weight (kDa)

8.38

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 80 - 592 2.5e-223 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1661
AatII GACGTC 1 cut(s) 21
AccBSI CCGCTC 2 cut(s) 123, 340
AccII CGCG 1 cut(s) 183
AciI CCGC 7 cut(s) 118, 121, 181, 332, 340, 343, 510
AclWI GGATC 4 cut(s) 471, 926, 1058, 1596
AcsI RAATTY 5 cut(s) 137, 292, 1256, 1333, 1360
AcuI CTGAAG 2 cut(s) 936, 1272
AcyI GRCGYC 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 66
AfaI GTAC 4 cut(s) 347, 545, 905, 1522
AfiI CCNNNNNNNGG 6 cut(s) 152, 371, 512, 513, 1228, 1695
AflII CTTAAG 1 cut(s) 569
AjiI CACGTC 1 cut(s) 1137
AjnI CCWGG 3 cut(s) 69, 886, 1227
AluBI AGCT 6 cut(s) 24, 277, 536, 805, 868, 1369
AluI AGCT 6 cut(s) 24, 277, 536, 805, 868, 1369
Alw26I GTCTC 5 cut(s) 80, 257, 447, 1612, 1638
AlwI GGATC 4 cut(s) 471, 926, 1058, 1596
Ama87I CYCGRG 1 cut(s) 1725
AoxI GGCC 7 cut(s) 55, 186, 192, 395, 423, 1026, 1196
ApeKI GCWGC 2 cut(s) 822, 998
ApoI RAATTY 5 cut(s) 137, 292, 1256, 1333, 1360
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspS9I GGNCC 5 cut(s) 99, 159, 382, 1026, 1071
AsuC2I CCSGG 4 cut(s) 111, 146, 386, 507
AsuHPI GGTGA 4 cut(s) 157, 400, 500, 1760
AvaI CYCGRG 1 cut(s) 1725
AvaII GGWCC 4 cut(s) 99, 159, 382, 1071
AxyI CCTNAGG 1 cut(s) 1744
BaeGI GKGCMC 1 cut(s) 770
BanII GRGCYC 2 cut(s) 116, 201
BbsI GAAGAC 1 cut(s) 1139
BbvI GCAGC 2 cut(s) 809, 1010
BccI CCATC 4 cut(s) 177, 387, 469, 842
BceAI ACGGC 2 cut(s) 109, 335
BciT130I CCWGG 3 cut(s) 71, 888, 1229
BciVI GTATCC 2 cut(s) 368, 1605
BcnI CCSGG 4 cut(s) 111, 146, 386, 507
BcoDI GTCTC 5 cut(s) 80, 257, 447, 1612, 1638
BfaI CTAG 3 cut(s) 789, 1272, 1792
BfrI CTTAAG 1 cut(s) 569
BfuI GTATCC 2 cut(s) 368, 1605
BglI GCCNNNNNGGC 2 cut(s) 340, 1204
BglII AGATCT 1 cut(s) 948
BisI GCNGC 5 cut(s) 121, 181, 341, 823, 999
BlsI GCNGC 5 cut(s) 122, 182, 342, 824, 1000
Bme1390I CCNGG 7 cut(s) 71, 111, 146, 386, 507, 888, 1229
Bme18I GGWCC 4 cut(s) 99, 159, 382, 1071
BmeT110I CYCGRG 1 cut(s) 1725
BmgBI CACGTC 1 cut(s) 1137
BmgT120I GGNCC 5 cut(s) 99, 159, 382, 1026, 1071
BmiI GGNNCC 5 cut(s) 115, 160, 161, 384, 1073
BmrFI CCNGG 7 cut(s) 71, 111, 146, 386, 507, 888, 1229
BmsI GCATC 4 cut(s) 555, 1099, 1565, 1723
BpiI GAAGAC 1 cut(s) 1139
BpmI CTGGAG 3 cut(s) 552, 909, 1250
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 4 cut(s) 111, 146, 386, 507
BsaAI YACGTR 1 cut(s) 1520
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 80, 1612
BsaJI CCNNGG 5 cut(s) 70, 189, 505, 1067, 1193
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 6 cut(s) 152, 371, 512, 513, 1228, 1695
Bse1I ACTGG 4 cut(s) 535, 1307, 1496, 1709
Bse21I CCTNAGG 1 cut(s) 1744
Bse3DI GCAATG 3 cut(s) 619, 675, 811
BseBI CCWGG 3 cut(s) 71, 888, 1229
BseDI CCNNGG 5 cut(s) 70, 189, 505, 1067, 1193
BseGI GGATG 5 cut(s) 429, 480, 1580, 1705, 1792
BseLI CCNNNNNNNGG 6 cut(s) 152, 371, 512, 513, 1228, 1695
BseMI GCAATG 3 cut(s) 619, 675, 811
BseMII CTCAG 3 cut(s) 1214, 1607, 1758
BseNI ACTGG 4 cut(s) 535, 1307, 1496, 1709
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 770
BseXI GCAGC 2 cut(s) 809, 1010
BsgI GTGCAG 1 cut(s) 1602
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 7 cut(s) 57, 188, 194, 397, 425, 1028, 1198
BsiHKCI CYCGRG 1 cut(s) 1725
BsiSI CCGG 6 cut(s) 110, 146, 335, 386, 507, 826
BslFI GGGAC 5 cut(s) 145, 312, 368, 529, 670
BslI CCNNNNNNNGG 6 cut(s) 152, 371, 512, 513, 1228, 1695
BsmAI GTCTC 5 cut(s) 80, 257, 447, 1612, 1638
BsmBI CGTCTC 1 cut(s) 447
BsmFI GGGAC 5 cut(s) 145, 312, 368, 529, 670
BsmI GAATGC 2 cut(s) 210, 961
BsnI GGCC 7 cut(s) 57, 188, 194, 397, 425, 1028, 1198
Bso31I GGTCTC 2 cut(s) 80, 1612
BsoBI CYCGRG 1 cut(s) 1725
Bsp1286I GDGCHC 3 cut(s) 116, 201, 770
Bsp1407I TGTACA 1 cut(s) 903
BspACI CCGC 7 cut(s) 118, 121, 181, 332, 340, 343, 510
BspANI GGCC 7 cut(s) 57, 188, 194, 397, 425, 1028, 1198
BspCNI CTCAG 3 cut(s) 1213, 1608, 1757
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 2 cut(s) 928, 1486
BspLI GGNNCC 5 cut(s) 115, 160, 161, 384, 1073
BspPI GGATC 4 cut(s) 471, 926, 1058, 1596
BspTI CTTAAG 1 cut(s) 569
BspTNI GGTCTC 2 cut(s) 80, 1612
BsrBI CCGCTC 2 cut(s) 123, 340
BsrDI GCAATG 3 cut(s) 619, 675, 811
BsrGI TGTACA 1 cut(s) 903
BsrI ACTGG 4 cut(s) 535, 1307, 1496, 1709
BssECI CCNNGG 5 cut(s) 70, 189, 505, 1067, 1193
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 1067, 1193
Bst2UI CCWGG 3 cut(s) 71, 888, 1229
Bst4CI ACNGT 4 cut(s) 64, 369, 599, 1483
Bst6I CTCTTC 3 cut(s) 447, 1052, 1746
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 569
BstAUI TGTACA 1 cut(s) 903
BstBAI YACGTR 1 cut(s) 1520
BstC8I GCNNGC 1 cut(s) 807
BstDEI CTNAG 4 cut(s) 881, 1200, 1616, 1744
BstF5I GGATG 5 cut(s) 429, 480, 1580, 1705, 1792
BstFNI CGCG 1 cut(s) 183
BstMAI GTCTC 5 cut(s) 80, 257, 447, 1612, 1638
BstMWI GCNNNNNNNGC 4 cut(s) 120, 340, 822, 1204
BstNI CCWGG 3 cut(s) 71, 888, 1229
BstNSI RCATGY 1 cut(s) 1541
BstSCI CCNGG 7 cut(s) 69, 109, 144, 384, 505, 886, 1227
BstSLI GKGCMC 1 cut(s) 770
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 809, 1010
BstV2I GAAGAC 1 cut(s) 1139
BstX2I RGATCY 3 cut(s) 948, 1063, 1588
BstYI RGATCY 3 cut(s) 948, 1063, 1588
Bsu36I CCTNAGG 1 cut(s) 1744
BsuI GTATCC 2 cut(s) 368, 1605
BsuRI GGCC 7 cut(s) 57, 188, 194, 397, 425, 1028, 1198
BtrI CACGTC 1 cut(s) 1137
BtsCI GGATG 5 cut(s) 429, 480, 1580, 1705, 1792
BtsI GCAGTG 1 cut(s) 1585
BtsIMutI CAGTG 3 cut(s) 60, 1034, 1585
Cac8I GCNNGC 1 cut(s) 807
CciI TCATGA 2 cut(s) 928, 1486
Cfr13I GGNCC 5 cut(s) 99, 159, 382, 1026, 1071
Csp6I GTAC 4 cut(s) 346, 544, 904, 1521
CspCI CAANNNNNGTGG 2 cut(s) 580, 615
CviQI GTAC 4 cut(s) 346, 544, 904, 1521
DdeI CTNAG 4 cut(s) 881, 1200, 1616, 1744
DraIII CACNNNGTG 1 cut(s) 66
Eam1104I CTCTTC 3 cut(s) 447, 1052, 1746
EarI CTCTTC 3 cut(s) 447, 1052, 1746
EciI GGCGGA 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 1067, 1193
Eco24I GRGCYC 2 cut(s) 116, 201
Eco31I GGTCTC 2 cut(s) 80, 1612
Eco47I GGWCC 4 cut(s) 99, 159, 382, 1071
Eco57I CTGAAG 2 cut(s) 936, 1272
Eco81I CCTNAGG 1 cut(s) 1744
Eco88I CYCGRG 1 cut(s) 1725
EcoO109I RGGNCCY 2 cut(s) 159, 382
EcoRI GAATTC 3 cut(s) 137, 1256, 1360
EcoRII CCWGG 3 cut(s) 69, 886, 1227
EcoT14I CCWWGG 2 cut(s) 1067, 1193
EcoT22I ATGCAT 1 cut(s) 1543
EcoT38I GRGCYC 2 cut(s) 116, 201
ErhI CCWWGG 2 cut(s) 1067, 1193
Esp3I CGTCTC 1 cut(s) 447
FalI AAGNNNNNCTT 2 cut(s) 766, 798
FaqI GGGAC 5 cut(s) 145, 312, 368, 529, 670
FauI CCCGC 2 cut(s) 125, 339
FauNDI CATATG 2 cut(s) 1277, 1525
Fnu4HI GCNGC 5 cut(s) 121, 181, 341, 823, 999
FokI GGATG 4 cut(s) 416, 487, 1587, 1712
FriOI GRGCYC 2 cut(s) 116, 201
Fsp4HI GCNGC 5 cut(s) 121, 181, 341, 823, 999
FspBI CTAG 3 cut(s) 789, 1272, 1792
GluI GCNGC 5 cut(s) 121, 181, 341, 823, 999
GsuI CTGGAG 3 cut(s) 552, 909, 1250
HaeIII GGCC 7 cut(s) 57, 188, 194, 397, 425, 1028, 1198
HapII CCGG 6 cut(s) 110, 146, 335, 386, 507, 826
Hin1I GRCGYC 1 cut(s) 18
HinfI GANTC 4 cut(s) 712, 742, 852, 1290
HpaII CCGG 6 cut(s) 110, 146, 335, 386, 507, 826
HphI GGTGA 4 cut(s) 157, 400, 500, 1760
Hpy166II GTNNAC 2 cut(s) 904, 906
Hpy188III TCNNGA 9 cut(s) 254, 578, 620, 914, 929, 935, 952, 1250, 1487
Hpy8I GTNNAC 2 cut(s) 904, 906
Hpy99I CGWCG 2 cut(s) 177, 180
HpyAV CCTTC 3 cut(s) 112, 280, 415
HpyCH4III ACNGT 4 cut(s) 64, 369, 599, 1483
HpyCH4IV ACGT 5 cut(s) 18, 440, 1136, 1519, 1570
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 340, 822, 1204
HpyF3I CTNAG 4 cut(s) 881, 1200, 1616, 1744
HpySE526I ACGT 5 cut(s) 18, 440, 1136, 1519, 1570
Hsp92I GRCGYC 1 cut(s) 18
KflI GGGWCCC 1 cut(s) 159
LmnI GCTCC 8 cut(s) 112, 119, 128, 307, 337, 533, 697, 1429
Lsp1109I GCAGC 2 cut(s) 809, 1010
LweI GCATC 4 cut(s) 555, 1099, 1565, 1723
MaeI CTAG 3 cut(s) 789, 1272, 1792
MaeII ACGT 5 cut(s) 18, 440, 1136, 1519, 1570
MaeIII GTNAC 4 cut(s) 488, 876, 1049, 1681
MbiI CCGCTC 2 cut(s) 123, 340
MboII GAAGA 8 cut(s) 146, 464, 650, 1069, 1144, 1265, 1523, 1763
MfeI CAATTG 1 cut(s) 349
MflI RGATCY 3 cut(s) 948, 1063, 1588
MhlI GDGCHC 3 cut(s) 116, 201, 770
MlyI GAGTC 1 cut(s) 1299
MmeI TCCRAC 1 cut(s) 150
Mph1103I ATGCAT 1 cut(s) 1543
MseI TTAA 3 cut(s) 570, 585, 1448
MslI CAYNNNNRTG 3 cut(s) 12, 680, 1355
MspA1I CMGCKG 1 cut(s) 277
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 6 cut(s) 110, 146, 335, 386, 507, 826
MspR9I CCNGG 7 cut(s) 71, 111, 146, 386, 507, 888, 1229
MunI CAATTG 1 cut(s) 349
Mva1269I GAATGC 2 cut(s) 210, 961
MvaI CCWGG 3 cut(s) 71, 888, 1229
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 120, 340, 822, 1204
NciI CCSGG 4 cut(s) 111, 146, 386, 507
NdeI CATATG 2 cut(s) 1277, 1525
NlaIV GGNNCC 5 cut(s) 115, 160, 161, 384, 1073
NmeAIII GCCGAG 3 cut(s) 33, 214, 220
NmuCI GTSAC 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 1543
NspI RCATGY 1 cut(s) 1541
PaeR7I CTCGAG 1 cut(s) 1725
PagI TCATGA 2 cut(s) 928, 1486
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 2 cut(s) 210, 961
PfeI GAWTC 3 cut(s) 712, 742, 852
PkrI GCNGC 5 cut(s) 122, 182, 342, 824, 1000
PleI GAGTC 1 cut(s) 1298
PpsI GAGTC 1 cut(s) 1298
Ppu21I YACGTR 1 cut(s) 1520
PpuMI RGGWCCY 2 cut(s) 159, 382
PsiI TTATAA 1 cut(s) 1661
Psp5II RGGWCCY 2 cut(s) 159, 382
Psp6I CCWGG 3 cut(s) 69, 886, 1227
PspGI CCWGG 3 cut(s) 69, 886, 1227
PspN4I GGNNCC 5 cut(s) 115, 160, 161, 384, 1073
PspPI GGNCC 5 cut(s) 99, 159, 382, 1026, 1071
PspPPI RGGWCCY 2 cut(s) 159, 382
PspXI VCTCGAGB 1 cut(s) 1725
PsuI RGATCY 3 cut(s) 948, 1063, 1588
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 4 cut(s) 347, 545, 905, 1522
RsaNI GTAC 4 cut(s) 346, 544, 904, 1521
RseI CAYNNNNRTG 3 cut(s) 12, 680, 1355
SaqAI TTAA 3 cut(s) 570, 585, 1448
SatI GCNGC 5 cut(s) 121, 181, 341, 823, 999
Sau96I GGNCC 5 cut(s) 99, 159, 382, 1026, 1071
SchI GAGTC 1 cut(s) 1299
ScrFI CCNGG 7 cut(s) 71, 111, 146, 386, 507, 888, 1229
SduI GDGCHC 3 cut(s) 116, 201, 770
SfaNI GCATC 4 cut(s) 555, 1099, 1565, 1723
Sfr274I CTCGAG 1 cut(s) 1725
SinI GGWCC 4 cut(s) 99, 159, 382, 1071
SlaI CTCGAG 1 cut(s) 1725
SmiMI CAYNNNNRTG 3 cut(s) 12, 680, 1355
SmlI CTYRAG 3 cut(s) 38, 569, 1725
SmoI CTYRAG 3 cut(s) 38, 569, 1725
SsiI CCGC 7 cut(s) 118, 121, 181, 332, 340, 343, 510
SspI AATATT 2 cut(s) 607, 1151
SspMI CTAG 3 cut(s) 789, 1272, 1792
StyD4I CCNGG 7 cut(s) 69, 109, 144, 384, 505, 886, 1227
StyI CCWWGG 2 cut(s) 1067, 1193
TaaI ACNGT 4 cut(s) 64, 369, 599, 1483
TaiI ACGT 5 cut(s) 21, 443, 1139, 1522, 1573
TaqI TCGA 3 cut(s) 141, 255, 1726
TatI WGTACW 2 cut(s) 543, 903
TauI GCSGC 3 cut(s) 123, 183, 343
TfiI GAWTC 3 cut(s) 712, 742, 852
Tru1I TTAA 3 cut(s) 570, 585, 1448
Tru9I TTAA 3 cut(s) 570, 585, 1448
TscAI CASTG 3 cut(s) 67, 1041, 1585
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 2 cut(s) 822, 998
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 9 cut(s) 17, 330, 426, 1145, 1326, 1328, 1373, 1430, 1475
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 3 cut(s) 67, 1041, 1585
Vha464I CTTAAG 1 cut(s) 569
VpaK11BI GGWCC 4 cut(s) 99, 159, 382, 1071
XapI RAATTY 5 cut(s) 137, 292, 1256, 1333, 1360
XceI RCATGY 1 cut(s) 1541
XcmI CCANNNNNNNNNTGG 1 cut(s) 1113
XhoI CTCGAG 1 cut(s) 1725
XspI CTAG 3 cut(s) 789, 1272, 1792
ZraI GACGTC 1 cut(s) 19
Zsp2I ATGCAT 1 cut(s) 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.