Rh2AG648800

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010488
Physical Location & Seq
Reverse (-)
86829231 .. 86831695
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG648800.1

Sequence Viewer

Length: 1791 bp
ATGAAGCACAGGAATGACGTCAGCTTCCCCAAGTTGCTCAAGTCCGTTCTGCTCGGCCTCACTGTGTGCTTGGGTCTCCTCTGTTTCTACTACGGCTGGTCCTTCGCTCCGGGATCCCGCCGCTCGGACCACGAAGAATTCGACCCGGTTTTCGGTGGGTCCCTCTCCCGTCGTAGCCGTGAGGCCGAGGCCGAGCCCCCCAAAAGCATTCCTATTTGTGATATGCAATTCTCGGAGTTGATACCTTGTCTCGATAGAAACCTCATTTACCAGCTGAAATTGAAGGTAAATTTGACACTTATGGAGCATTATGAACGGCATTGTCCCCCGCCGGAGCGGCGGTACAATTGCTTGATACCACCACCATTAGGATACAAGGTCCCGGTGAGATGGCCTACGAGTAGAGATGAAGTGTGGAAGGCCAACATCCCCCATACGTCTCTTGCAGAAGAGAAATCGGATCAGAATTGGATGGTTGTGAATGGTGACAAGATCAATTTCCCCGGCGGAGGGACACATTTCCATACTGGAGCTGATAAGTACATTGTTGCTCTTTCCAAGATGCTTAAGTTTCCTGATTTTAAGTTCAACAACGGTGGAAATATTCGCAATGTTCTTGATGTGGGTTGTGGAGTTGCAAGTTTTGGGGCGTATCTTCTTTCTCATCATGTCATTGCCATGTCCCTTGCTCCTAATGATGTGCATGAGAATCAAATACAATTTGCATTAGAGAGGGGGATTCCAGCAACTCTTGGTATCTTGGGTACAAAAAGACTTACTTATCCTAGCAGATCATTTGAGCTGGCTCATTGCTCACGCTGCCGGATAGATTGGCTTCAAAGAGATGGAATCCTCTTATTAGAGCTTGACAGGTTACTAAGACCTGGAGGGTATTTTGTGTACACTTCTCCTGAAGCATATGCTCATGATCCTGAAAATCAAAAGATCTGGAATGCTATGTCTGATCTTTCAAAAAGAATGTGCTGGAAAGTTGCAGCCAAGAAAGAGCAGAGTGTTGTATGGGCCAAGCCACTGACTAATAGTTGTTACTTGAAGAGAGATCCTTGGACCCAACCCCCTCTGTGCAATTCTGATGATGACCCAGATGCAATTTGGAATGTGAGCATGAAGACGTGCATTTCTCAATATTCTGCAAAGGTGCATAGGGATAGAGGAAGCAGACTTGCCCCTTGGCCTCAGAGGCTTACGACACCACCCCCTCGCCTGGAGGAAATTGGTGTCAGTCCTGAAGAATTCCAAGAGGACACTAGCATATGGCATTTTAGAGTCATTGAATACTGGAAGCAGATGAAGTCTGTCATACAGAGAAATTCATTCAGAAATGTCATGGATATGAATTCACAGCTTGGTGGATTTGCTACTGCCTTGAATGGAAAAGATGTCTGGGTTATGAATGTTGCTCCTGTCCATGTATCTGCCCAATTAAAGATTGTTTATGATCGGGGCTTGATTGGAACTGTTCATGACTGGTGTGAAGCGTTTTCCACATACCCACGTACATATGATCTTCTACACGCATGGGCAGTATTTTCAGATATTGAGGAACGTGGATGCAGTGCAGAGGATCTATTGATTGAAATGGATAGGATACTGAGACCCAATGGGTTTGTCATCATAAGAGACAAACCCTCTGTTATAAACTACATCCGAAAGTTTGTGACTGCCTTGAAGTGGGATGACTGGTTATCAGAAGTTGAACCTCGAGTTGATGCTCTTTCCTCAGGTGAAGAGAGAGTTTTGATTGCAAGAAAGAAGTTGTGGGATGACTAG

Protein Analysis

596

Amino Acids

68.37

Weight (kDa)

7.85

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 79 - 591 2.3e-223 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1658
AatII GACGTC 1 cut(s) 21
AccBSI CCGCTC 2 cut(s) 123, 337
AciI CCGC 6 cut(s) 118, 121, 329, 337, 340, 507
AclWI GGATC 6 cut(s) 108, 121, 468, 923, 1055, 1593
AcsI RAATTY 5 cut(s) 137, 289, 1253, 1330, 1357
AcuI CTGAAG 2 cut(s) 933, 1269
AcyI GRCGYC 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 66
AfaI GTAC 5 cut(s) 344, 542, 766, 902, 1519
AfiI CCNNNNNNNGG 7 cut(s) 124, 152, 368, 509, 510, 1225, 1692
AflII CTTAAG 1 cut(s) 566
AjiI CACGTC 1 cut(s) 1134
AjnI CCWGG 2 cut(s) 883, 1224
AluBI AGCT 6 cut(s) 24, 274, 533, 802, 865, 1366
AluI AGCT 6 cut(s) 24, 274, 533, 802, 865, 1366
Alw26I GTCTC 5 cut(s) 80, 254, 444, 1609, 1635
AlwI GGATC 6 cut(s) 108, 121, 468, 923, 1055, 1593
Ama87I CYCGRG 1 cut(s) 1722
AoxI GGCC 7 cut(s) 55, 183, 189, 392, 420, 1023, 1193
ApeKI GCWGC 2 cut(s) 819, 995
ApoI RAATTY 5 cut(s) 137, 289, 1253, 1330, 1357
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspS9I GGNCC 6 cut(s) 99, 127, 159, 379, 1023, 1068
AsuC2I CCSGG 4 cut(s) 111, 146, 383, 504
AsuHPI GGTGA 3 cut(s) 397, 497, 1757
AvaI CYCGRG 1 cut(s) 1722
AvaII GGWCC 5 cut(s) 99, 127, 159, 379, 1068
AxyI CCTNAGG 1 cut(s) 1741
BamHI GGATCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 1136
BbvI GCAGC 2 cut(s) 806, 1007
BccI CCATC 3 cut(s) 384, 466, 839
BceAI ACGGC 3 cut(s) 109, 162, 332
BciT130I CCWGG 2 cut(s) 885, 1226
BciVI GTATCC 2 cut(s) 365, 1602
BcnI CCSGG 4 cut(s) 111, 146, 383, 504
BcoDI GTCTC 5 cut(s) 80, 254, 444, 1609, 1635
BfaI CTAG 3 cut(s) 786, 1269, 1789
BfrI CTTAAG 1 cut(s) 566
BfuI GTATCC 2 cut(s) 365, 1602
BglI GCCNNNNNGGC 2 cut(s) 337, 1201
BglII AGATCT 1 cut(s) 945
BisI GCNGC 4 cut(s) 121, 338, 820, 996
BlsI GCNGC 4 cut(s) 122, 339, 821, 997
Bme1390I CCNGG 6 cut(s) 111, 146, 383, 504, 885, 1226
Bme18I GGWCC 5 cut(s) 99, 127, 159, 379, 1068
BmeT110I CYCGRG 1 cut(s) 1722
BmgBI CACGTC 1 cut(s) 1134
BmgT120I GGNCC 6 cut(s) 99, 127, 159, 379, 1023, 1068
BmiI GGNNCC 5 cut(s) 115, 160, 161, 381, 1070
BmrFI CCNGG 6 cut(s) 111, 146, 383, 504, 885, 1226
BmsI GCATC 4 cut(s) 552, 1096, 1562, 1720
BpiI GAAGAC 1 cut(s) 1136
BpmI CTGGAG 3 cut(s) 549, 906, 1247
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 4 cut(s) 111, 146, 383, 504
BsaAI YACGTR 1 cut(s) 1517
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 80, 1609
BsaJI CCNNGG 4 cut(s) 186, 502, 1064, 1190
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 7 cut(s) 124, 152, 368, 509, 510, 1225, 1692
Bse1I ACTGG 4 cut(s) 532, 1304, 1493, 1706
Bse21I CCTNAGG 1 cut(s) 1741
Bse3DI GCAATG 3 cut(s) 616, 672, 808
BseBI CCWGG 2 cut(s) 885, 1226
BseDI CCNNGG 4 cut(s) 186, 502, 1064, 1190
BseGI GGATG 6 cut(s) 426, 477, 1577, 1665, 1702, 1789
BseLI CCNNNNNNNGG 7 cut(s) 124, 152, 368, 509, 510, 1225, 1692
BseMI GCAATG 3 cut(s) 616, 672, 808
BseMII CTCAG 3 cut(s) 1211, 1604, 1755
BseNI ACTGG 4 cut(s) 532, 1304, 1493, 1706
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 806, 1007
BsgI GTGCAG 1 cut(s) 1599
BshFI GGCC 7 cut(s) 57, 185, 191, 394, 422, 1025, 1195
BsiHKCI CYCGRG 1 cut(s) 1722
BsiSI CCGG 6 cut(s) 110, 146, 332, 383, 504, 823
BslFI GGGAC 5 cut(s) 145, 309, 365, 526, 667
BslI CCNNNNNNNGG 7 cut(s) 124, 152, 368, 509, 510, 1225, 1692
BsmAI GTCTC 5 cut(s) 80, 254, 444, 1609, 1635
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 5 cut(s) 145, 309, 365, 526, 667
BsmI GAATGC 2 cut(s) 207, 958
BsnI GGCC 7 cut(s) 57, 185, 191, 394, 422, 1025, 1195
Bso31I GGTCTC 2 cut(s) 80, 1609
BsoBI CYCGRG 1 cut(s) 1722
Bsp1286I GDGCHC 1 cut(s) 198
Bsp1407I TGTACA 1 cut(s) 900
BspACI CCGC 6 cut(s) 118, 121, 329, 337, 340, 507
BspANI GGCC 7 cut(s) 57, 185, 191, 394, 422, 1025, 1195
BspCNI CTCAG 3 cut(s) 1210, 1605, 1754
BspHI TCATGA 2 cut(s) 925, 1483
BspLI GGNNCC 5 cut(s) 115, 160, 161, 381, 1070
BspPI GGATC 6 cut(s) 108, 121, 468, 923, 1055, 1593
BspTI CTTAAG 1 cut(s) 566
BspTNI GGTCTC 2 cut(s) 80, 1609
BsrBI CCGCTC 2 cut(s) 123, 337
BsrDI GCAATG 3 cut(s) 616, 672, 808
BsrGI TGTACA 1 cut(s) 900
BsrI ACTGG 4 cut(s) 532, 1304, 1493, 1706
BssECI CCNNGG 4 cut(s) 186, 502, 1064, 1190
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 1064, 1190
Bst2UI CCWGG 2 cut(s) 885, 1226
Bst4CI ACNGT 3 cut(s) 64, 596, 1480
Bst6I CTCTTC 3 cut(s) 444, 1049, 1743
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 566
BstAUI TGTACA 1 cut(s) 900
BstBAI YACGTR 1 cut(s) 1517
BstC8I GCNNGC 1 cut(s) 804
BstDEI CTNAG 4 cut(s) 878, 1197, 1613, 1741
BstF5I GGATG 6 cut(s) 426, 477, 1577, 1665, 1702, 1789
BstMAI GTCTC 5 cut(s) 80, 254, 444, 1609, 1635
BstMWI GCNNNNNNNGC 3 cut(s) 337, 819, 1201
BstNI CCWGG 2 cut(s) 885, 1226
BstSCI CCNGG 6 cut(s) 109, 144, 381, 502, 883, 1224
BstV1I GCAGC 2 cut(s) 806, 1007
BstV2I GAAGAC 1 cut(s) 1136
BstX2I RGATCY 4 cut(s) 113, 945, 1060, 1585
BstYI RGATCY 4 cut(s) 113, 945, 1060, 1585
Bsu36I CCTNAGG 1 cut(s) 1741
BsuI GTATCC 2 cut(s) 365, 1602
BsuRI GGCC 7 cut(s) 57, 185, 191, 394, 422, 1025, 1195
BtrI CACGTC 1 cut(s) 1134
BtsCI GGATG 6 cut(s) 426, 477, 1577, 1665, 1702, 1789
BtsI GCAGTG 1 cut(s) 1582
BtsIMutI CAGTG 3 cut(s) 60, 1031, 1582
Cac8I GCNNGC 1 cut(s) 804
CciI TCATGA 2 cut(s) 925, 1483
Cfr13I GGNCC 6 cut(s) 99, 127, 159, 379, 1023, 1068
Csp6I GTAC 5 cut(s) 343, 541, 765, 901, 1518
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 9 cut(s) 668, 679, 704, 926, 1126, 1348, 1430, 1484, 1539
CviQI GTAC 5 cut(s) 343, 541, 765, 901, 1518
DdeI CTNAG 4 cut(s) 878, 1197, 1613, 1741
DraIII CACNNNGTG 1 cut(s) 66
Eam1104I CTCTTC 3 cut(s) 444, 1049, 1743
EarI CTCTTC 3 cut(s) 444, 1049, 1743
EciI GGCGGA 1 cut(s) 522
Eco130I CCWWGG 2 cut(s) 1064, 1190
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 2 cut(s) 80, 1609
Eco47I GGWCC 5 cut(s) 99, 127, 159, 379, 1068
Eco57I CTGAAG 2 cut(s) 933, 1269
Eco81I CCTNAGG 1 cut(s) 1741
Eco88I CYCGRG 1 cut(s) 1722
EcoO109I RGGNCCY 2 cut(s) 159, 379
EcoRI GAATTC 3 cut(s) 137, 1253, 1357
EcoRII CCWGG 2 cut(s) 883, 1224
EcoT14I CCWWGG 2 cut(s) 1064, 1190
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 2 cut(s) 1064, 1190
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 9 cut(s) 671, 682, 707, 929, 1129, 1351, 1433, 1487, 1542
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FaqI GGGAC 5 cut(s) 145, 309, 365, 526, 667
FatI CATG 9 cut(s) 667, 678, 703, 925, 1125, 1347, 1429, 1483, 1538
FauI CCCGC 2 cut(s) 125, 336
FauNDI CATATG 3 cut(s) 919, 1274, 1522
Fnu4HI GCNGC 4 cut(s) 121, 338, 820, 996
FokI GGATG 5 cut(s) 413, 484, 1584, 1652, 1709
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 4 cut(s) 121, 338, 820, 996
FspBI CTAG 3 cut(s) 786, 1269, 1789
GluI GCNGC 4 cut(s) 121, 338, 820, 996
GsuI CTGGAG 3 cut(s) 549, 906, 1247
HaeIII GGCC 7 cut(s) 57, 185, 191, 394, 422, 1025, 1195
HapII CCGG 6 cut(s) 110, 146, 332, 383, 504, 823
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 9 cut(s) 671, 682, 707, 929, 1129, 1351, 1433, 1487, 1542
HinfI GANTC 4 cut(s) 709, 739, 849, 1287
HpaII CCGG 6 cut(s) 110, 146, 332, 383, 504, 823
HphI GGTGA 3 cut(s) 397, 497, 1757
Hpy166II GTNNAC 2 cut(s) 901, 903
Hpy188III TCNNGA 9 cut(s) 251, 575, 617, 911, 926, 932, 949, 1247, 1484
Hpy8I GTNNAC 2 cut(s) 901, 903
Hpy99I CGWCG 1 cut(s) 174
HpyAV CCTTC 3 cut(s) 112, 277, 412
HpyCH4III ACNGT 3 cut(s) 64, 596, 1480
HpyCH4IV ACGT 5 cut(s) 18, 437, 1133, 1516, 1567
HpyF10VI GCNNNNNNNGC 3 cut(s) 337, 819, 1201
HpyF3I CTNAG 4 cut(s) 878, 1197, 1613, 1741
HpySE526I ACGT 5 cut(s) 18, 437, 1133, 1516, 1567
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 9 cut(s) 671, 682, 707, 929, 1129, 1351, 1433, 1487, 1542
KflI GGGWCCC 1 cut(s) 159
LmnI GCTCC 6 cut(s) 112, 304, 334, 530, 694, 1426
Lsp1109I GCAGC 2 cut(s) 806, 1007
LweI GCATC 4 cut(s) 552, 1096, 1562, 1720
MaeI CTAG 3 cut(s) 786, 1269, 1789
MaeII ACGT 5 cut(s) 18, 437, 1133, 1516, 1567
MaeIII GTNAC 4 cut(s) 485, 873, 1046, 1678
MbiI CCGCTC 2 cut(s) 123, 337
MboII GAAGA 8 cut(s) 146, 461, 647, 1066, 1141, 1262, 1520, 1760
MfeI CAATTG 1 cut(s) 346
MflI RGATCY 4 cut(s) 113, 945, 1060, 1585
MhlI GDGCHC 1 cut(s) 198
MlyI GAGTC 1 cut(s) 1296
MseI TTAA 3 cut(s) 567, 582, 1445
MslI CAYNNNNRTG 3 cut(s) 12, 677, 1352
MspA1I CMGCKG 1 cut(s) 274
MspCI CTTAAG 1 cut(s) 566
MspI CCGG 6 cut(s) 110, 146, 332, 383, 504, 823
MspR9I CCNGG 6 cut(s) 111, 146, 383, 504, 885, 1226
MunI CAATTG 1 cut(s) 346
Mva1269I GAATGC 2 cut(s) 207, 958
MvaI CCWGG 2 cut(s) 885, 1226
MwoI GCNNNNNNNGC 3 cut(s) 337, 819, 1201
NciI CCSGG 4 cut(s) 111, 146, 383, 504
NdeI CATATG 3 cut(s) 919, 1274, 1522
NlaIII CATG 9 cut(s) 671, 682, 707, 929, 1129, 1351, 1433, 1487, 1542
NlaIV GGNNCC 5 cut(s) 115, 160, 161, 381, 1070
NmeAIII GCCGAG 3 cut(s) 33, 211, 217
NmuCI GTSAC 2 cut(s) 485, 1678
PaeR7I CTCGAG 1 cut(s) 1722
PagI TCATGA 2 cut(s) 925, 1483
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 2 cut(s) 207, 958
PfeI GAWTC 3 cut(s) 709, 739, 849
PfoI TCCNGGA 1 cut(s) 109
PkrI GCNGC 4 cut(s) 122, 339, 821, 997
PleI GAGTC 1 cut(s) 1295
PpsI GAGTC 1 cut(s) 1295
Ppu21I YACGTR 1 cut(s) 1517
PpuMI RGGWCCY 2 cut(s) 159, 379
PsiI TTATAA 1 cut(s) 1658
Psp5II RGGWCCY 2 cut(s) 159, 379
Psp6I CCWGG 2 cut(s) 883, 1224
PspGI CCWGG 2 cut(s) 883, 1224
PspN4I GGNNCC 5 cut(s) 115, 160, 161, 381, 1070
PspPI GGNCC 6 cut(s) 99, 127, 159, 379, 1023, 1068
PspPPI RGGWCCY 2 cut(s) 159, 379
PspXI VCTCGAGB 1 cut(s) 1722
PsuI RGATCY 4 cut(s) 113, 945, 1060, 1585
PvuII CAGCTG 1 cut(s) 274
RsaI GTAC 5 cut(s) 344, 542, 766, 902, 1519
RsaNI GTAC 5 cut(s) 343, 541, 765, 901, 1518
RseI CAYNNNNRTG 3 cut(s) 12, 677, 1352
SaqAI TTAA 3 cut(s) 567, 582, 1445
SatI GCNGC 4 cut(s) 121, 338, 820, 996
Sau96I GGNCC 6 cut(s) 99, 127, 159, 379, 1023, 1068
SchI GAGTC 1 cut(s) 1296
ScrFI CCNGG 6 cut(s) 111, 146, 383, 504, 885, 1226
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 4 cut(s) 552, 1096, 1562, 1720
Sfr274I CTCGAG 1 cut(s) 1722
SinI GGWCC 5 cut(s) 99, 127, 159, 379, 1068
SlaI CTCGAG 1 cut(s) 1722
SmiMI CAYNNNNRTG 3 cut(s) 12, 677, 1352
SmlI CTYRAG 3 cut(s) 38, 566, 1722
SmoI CTYRAG 3 cut(s) 38, 566, 1722
SsiI CCGC 6 cut(s) 118, 121, 329, 337, 340, 507
SspI AATATT 2 cut(s) 604, 1148
SspMI CTAG 3 cut(s) 786, 1269, 1789
StyD4I CCNGG 6 cut(s) 109, 144, 381, 502, 883, 1224
StyI CCWWGG 2 cut(s) 1064, 1190
TaaI ACNGT 3 cut(s) 64, 596, 1480
TaiI ACGT 5 cut(s) 21, 440, 1136, 1519, 1570
TaqI TCGA 3 cut(s) 141, 252, 1723
TatI WGTACW 2 cut(s) 540, 900
TauI GCSGC 2 cut(s) 123, 340
TfiI GAWTC 3 cut(s) 709, 739, 849
Tru1I TTAA 3 cut(s) 567, 582, 1445
Tru9I TTAA 3 cut(s) 567, 582, 1445
TscAI CASTG 3 cut(s) 67, 1038, 1582
TseFI GTSAC 2 cut(s) 485, 1678
TseI GCWGC 2 cut(s) 819, 995
Tsp45I GTSAC 2 cut(s) 485, 1678
TspDTI ATGAA 9 cut(s) 17, 327, 423, 1142, 1323, 1325, 1370, 1427, 1472
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 3 cut(s) 67, 1038, 1582
Vha464I CTTAAG 1 cut(s) 566
VpaK11BI GGWCC 5 cut(s) 99, 127, 159, 379, 1068
XapI RAATTY 5 cut(s) 137, 289, 1253, 1330, 1357
XcmI CCANNNNNNNNNTGG 1 cut(s) 1110
XhoI CTCGAG 1 cut(s) 1722
XspI CTAG 3 cut(s) 786, 1269, 1789
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.