Rw2G053260

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010488
Physical Location & Seq
Reverse (-)
81060713 .. 81063183
2471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053260.1

Sequence Viewer

Length: 1788 bp
ATGAAGCACAGGAATGACGTCAGCTTCCCCAAGTTGCTCAAGTCCGTTCTGCTCGGCCTCACTGTGTGCCTGGGTCTCCTCTGTTTCTACTACGGCTGGTCCTTCGCTCCGGGCTCCCGCCGCTCCGACCACGAAGAATTCGACCCGGTTTTCGGTGGGTCCCTCGCCCATCGTCGTCGCCGCGAGGCCGAGGCCGAGGCCGAGCCCCCCAAAAGCATTCCTATTTGTGATATGCAATTCTCGGAGTTGATACCTTGTCTCGATAGAAACCTCATTTACCAGCTGAAATTGAAGGTAAATTTGACACTTATGGAGCATTATGAACGGCATTGTCCCCCGCCGGAGCGGCGGTACAATTGCTTGATACCACCACCGTTAGGATACAGATGGCCTACGAGTAGAGATGAAGTGTGGAAGGCCAACATCCCCCATACGTCTCTTGCAGAAGAGAAATCGGATCAGAATTGGATGGTTGTGAATGGTGACAAGATCAATTTCCCCGGCGGAGGGACACATTTCCATACTGGAGCTGATAAGTACATTGTTGCTCTTTCCAAGATGCTTAAGTTTCCTGATTTTAAGTTCAACAACGGTGGAAATATTCGCAATGTTCTTGATGTGGGTTGTGGAGTTGCAAGTTTTGGGGCGTATCTTCTTTCTCATCATGTCATTGCCATGTCCCTTGCTCCTAATGATGTGCATGAGAATCAAATACAATTTGCATTAGAGAGGGGGATTCCAGCAACTCTTGGTATCTTGGGCACAAAAAGACTTACTTATCCTAGCAGATCATTTGAGCTGGCTCATTGCTCACGCTGCCGGATAGATTGGCTTCAAAGAGATGGAATCCTCTTATTAGAGCTTGACAGGTTACTAAGACCTGGAGGGTATTTTGTGTACACTTCTCCTGAAGCGTATGCTCATGATCCTGAAAATCAAAAGATCTGGAATGCTATGTCTGATCTTTCAAAAAGAATGTGCTGGAAAGTTGCAGCCAAGAAAGATCAGAGTGTTGTATGGGCCAAGCCACTGACTAATAGTTGTTACTTGAAGAGAGATCCTTGGACCCAACCCCCTCTGTGCAATTCTGTTGATGACCCAGATGCAGTTTGGAATGTAAGCATGAAGACGTGCATTTCTCAATATTCTGCAAAGGTGCATAGGGATAGAGGAAGCAGACTTGCCCCTTGGCCTCAGAGGCTTACGACACCACCCCCTCGCCTGGAGGAAATTGGTGTCAGTCCTGAAGAATTCCAAGAGGACACTAGCATATGGCATTTTAGAGTCATTGAATACTGGAAGCAGATGAAGTCTGTCATACAGAGAAATTCATTCAGAAATGTCATGGATATGAATTCACAGCTTGGTGGATTTGCTACTGCCTTGAATGGAAAAGATGTCTGGGTTATGAATGTTGCTCCTGTCCATGTATCCGCCAAATTAAAGATTGTTTATGATCGGGGCTTGATTGGAACTGTTCATGACTGGTGTGAAGCGTTTTCCACATACCCACGTACATATGATCTTCTACATGCATGGGCAGTATTTTCAGATATTGAGGAACGTGGATGCAGTGCAGAGGATCTATTGATTGAAATGGATAGGATACTGAGACCCAATGGGTTTGTCATCATAAGAGACAAACCCTCTGTTATAAACTATATCCGAAAGTTTGTGACTGCCTTGAAGTGGGATGACTGGTTATCAGAAGTTGAACCTCGAGTTGATGCTCTTTCCTCAGGTGAAGAGAGAGTTTTGATTGCAAGAAAGAAGTTGTGGGATGACTAG

Protein Analysis

595

Amino Acids

68.29

Weight (kDa)

8.08

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 82 - 590 2.6e-218 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1655
AatII GACGTC 1 cut(s) 21
AccBSI CCGCTC 2 cut(s) 123, 346
AccII CGCG 1 cut(s) 183
AciI CCGC 8 cut(s) 118, 121, 181, 338, 346, 349, 504, 1434
AclWI GGATC 4 cut(s) 465, 920, 1052, 1590
AcsI RAATTY 5 cut(s) 137, 298, 1250, 1327, 1354
AcuI CTGAAG 2 cut(s) 930, 1266
AcyI GRCGYC 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 66
AfaI GTAC 4 cut(s) 353, 539, 899, 1516
AfiI CCNNNNNNNGG 6 cut(s) 152, 377, 506, 507, 1222, 1689
AflII CTTAAG 1 cut(s) 563
AjiI CACGTC 1 cut(s) 1131
AjnI CCWGG 3 cut(s) 69, 880, 1221
AluBI AGCT 6 cut(s) 24, 283, 530, 799, 862, 1363
AluI AGCT 6 cut(s) 24, 283, 530, 799, 862, 1363
Alw26I GTCTC 5 cut(s) 80, 263, 441, 1606, 1632
AlwI GGATC 4 cut(s) 465, 920, 1052, 1590
Ama87I CYCGRG 1 cut(s) 1719
AoxI GGCC 8 cut(s) 55, 186, 192, 198, 389, 417, 1020, 1190
ApeKI GCWGC 2 cut(s) 816, 992
ApoI RAATTY 5 cut(s) 137, 298, 1250, 1327, 1354
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspS9I GGNCC 4 cut(s) 99, 159, 1020, 1065
AsuC2I CCSGG 3 cut(s) 111, 146, 501
AsuHPI GGTGA 2 cut(s) 494, 1754
AvaI CYCGRG 1 cut(s) 1719
AvaII GGWCC 3 cut(s) 99, 159, 1065
AxyI CCTNAGG 1 cut(s) 1738
BaeGI GKGCMC 1 cut(s) 764
BanII GRGCYC 2 cut(s) 116, 207
BbsI GAAGAC 1 cut(s) 1133
BbvI GCAGC 2 cut(s) 803, 1004
BccI CCATC 4 cut(s) 177, 381, 463, 836
BceAI ACGGC 2 cut(s) 109, 341
BciT130I CCWGG 3 cut(s) 71, 882, 1223
BciVI GTATCC 3 cut(s) 374, 1441, 1599
BcnI CCSGG 3 cut(s) 111, 146, 501
BcoDI GTCTC 5 cut(s) 80, 263, 441, 1606, 1632
BfaI CTAG 3 cut(s) 783, 1266, 1786
BfrI CTTAAG 1 cut(s) 563
BfuI GTATCC 3 cut(s) 374, 1441, 1599
BglI GCCNNNNNGGC 2 cut(s) 346, 1198
BglII AGATCT 1 cut(s) 942
BisI GCNGC 5 cut(s) 121, 181, 347, 817, 993
BlsI GCNGC 5 cut(s) 122, 182, 348, 818, 994
Bme1390I CCNGG 6 cut(s) 71, 111, 146, 501, 882, 1223
Bme18I GGWCC 3 cut(s) 99, 159, 1065
BmeT110I CYCGRG 1 cut(s) 1719
BmgBI CACGTC 1 cut(s) 1131
BmgT120I GGNCC 4 cut(s) 99, 159, 1020, 1065
BmiI GGNNCC 4 cut(s) 115, 160, 161, 1067
BmrFI CCNGG 6 cut(s) 71, 111, 146, 501, 882, 1223
BmsI GCATC 4 cut(s) 549, 1093, 1559, 1717
BpiI GAAGAC 1 cut(s) 1133
BpmI CTGGAG 3 cut(s) 546, 903, 1244
BpuEI CTTGAG 1 cut(s) 23
BpuMI CCSGG 3 cut(s) 111, 146, 501
BsaAI YACGTR 1 cut(s) 1514
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 80, 1606
BsaJI CCNNGG 6 cut(s) 70, 189, 195, 499, 1061, 1187
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 6 cut(s) 152, 377, 506, 507, 1222, 1689
Bse1I ACTGG 4 cut(s) 529, 1301, 1490, 1703
Bse21I CCTNAGG 1 cut(s) 1738
Bse3DI GCAATG 3 cut(s) 613, 669, 805
BseBI CCWGG 3 cut(s) 71, 882, 1223
BseDI CCNNGG 6 cut(s) 70, 189, 195, 499, 1061, 1187
BseGI GGATG 5 cut(s) 423, 474, 1574, 1699, 1786
BseLI CCNNNNNNNGG 6 cut(s) 152, 377, 506, 507, 1222, 1689
BseMI GCAATG 3 cut(s) 613, 669, 805
BseMII CTCAG 3 cut(s) 1208, 1601, 1752
BseNI ACTGG 4 cut(s) 529, 1301, 1490, 1703
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 764
BseXI GCAGC 2 cut(s) 803, 1004
BsgI GTGCAG 1 cut(s) 1596
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 8 cut(s) 57, 188, 194, 200, 391, 419, 1022, 1192
BsiHKCI CYCGRG 1 cut(s) 1719
BsiSI CCGG 5 cut(s) 110, 146, 341, 501, 820
BslFI GGGAC 4 cut(s) 145, 318, 523, 664
BslI CCNNNNNNNGG 6 cut(s) 152, 377, 506, 507, 1222, 1689
BsmAI GTCTC 5 cut(s) 80, 263, 441, 1606, 1632
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 4 cut(s) 145, 318, 523, 664
BsmI GAATGC 2 cut(s) 216, 955
BsnI GGCC 8 cut(s) 57, 188, 194, 200, 391, 419, 1022, 1192
Bso31I GGTCTC 2 cut(s) 80, 1606
BsoBI CYCGRG 1 cut(s) 1719
Bsp1286I GDGCHC 3 cut(s) 116, 207, 764
Bsp1407I TGTACA 1 cut(s) 897
BspACI CCGC 8 cut(s) 118, 121, 181, 338, 346, 349, 504, 1434
BspANI GGCC 8 cut(s) 57, 188, 194, 200, 391, 419, 1022, 1192
BspCNI CTCAG 3 cut(s) 1207, 1602, 1751
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 2 cut(s) 922, 1480
BspLI GGNNCC 4 cut(s) 115, 160, 161, 1067
BspPI GGATC 4 cut(s) 465, 920, 1052, 1590
BspTI CTTAAG 1 cut(s) 563
BspTNI GGTCTC 2 cut(s) 80, 1606
BsrBI CCGCTC 2 cut(s) 123, 346
BsrDI GCAATG 3 cut(s) 613, 669, 805
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 4 cut(s) 529, 1301, 1490, 1703
BssECI CCNNGG 6 cut(s) 70, 189, 195, 499, 1061, 1187
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 1061, 1187
Bst2UI CCWGG 3 cut(s) 71, 882, 1223
Bst4CI ACNGT 4 cut(s) 64, 375, 593, 1477
Bst6I CTCTTC 3 cut(s) 441, 1046, 1740
BstACI GRCGYC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 897
BstBAI YACGTR 1 cut(s) 1514
BstC8I GCNNGC 1 cut(s) 801
BstDEI CTNAG 4 cut(s) 875, 1194, 1610, 1738
BstF5I GGATG 5 cut(s) 423, 474, 1574, 1699, 1786
BstFNI CGCG 1 cut(s) 183
BstMAI GTCTC 5 cut(s) 80, 263, 441, 1606, 1632
BstMWI GCNNNNNNNGC 4 cut(s) 120, 346, 816, 1198
BstNI CCWGG 3 cut(s) 71, 882, 1223
BstNSI RCATGY 1 cut(s) 1535
BstSCI CCNGG 6 cut(s) 69, 109, 144, 499, 880, 1221
BstSLI GKGCMC 1 cut(s) 764
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 803, 1004
BstV2I GAAGAC 1 cut(s) 1133
BstX2I RGATCY 3 cut(s) 942, 1057, 1582
BstYI RGATCY 3 cut(s) 942, 1057, 1582
Bsu36I CCTNAGG 1 cut(s) 1738
BsuI GTATCC 3 cut(s) 374, 1441, 1599
BsuRI GGCC 8 cut(s) 57, 188, 194, 200, 391, 419, 1022, 1192
BtrI CACGTC 1 cut(s) 1131
BtsCI GGATG 5 cut(s) 423, 474, 1574, 1699, 1786
BtsI GCAGTG 1 cut(s) 1579
BtsIMutI CAGTG 3 cut(s) 60, 1028, 1579
Cac8I GCNNGC 1 cut(s) 801
CciI TCATGA 2 cut(s) 922, 1480
Cfr13I GGNCC 4 cut(s) 99, 159, 1020, 1065
Csp6I GTAC 4 cut(s) 352, 538, 898, 1515
CspCI CAANNNNNGTGG 2 cut(s) 574, 609
CviQI GTAC 4 cut(s) 352, 538, 898, 1515
DdeI CTNAG 4 cut(s) 875, 1194, 1610, 1738
DraIII CACNNNGTG 1 cut(s) 66
Eam1104I CTCTTC 3 cut(s) 441, 1046, 1740
EarI CTCTTC 3 cut(s) 441, 1046, 1740
EciI GGCGGA 2 cut(s) 519, 1423
Eco130I CCWWGG 2 cut(s) 1061, 1187
Eco24I GRGCYC 2 cut(s) 116, 207
Eco31I GGTCTC 2 cut(s) 80, 1606
Eco47I GGWCC 3 cut(s) 99, 159, 1065
Eco57I CTGAAG 2 cut(s) 930, 1266
Eco81I CCTNAGG 1 cut(s) 1738
Eco88I CYCGRG 1 cut(s) 1719
EcoO109I RGGNCCY 1 cut(s) 159
EcoRI GAATTC 3 cut(s) 137, 1250, 1354
EcoRII CCWGG 3 cut(s) 69, 880, 1221
EcoT14I CCWWGG 2 cut(s) 1061, 1187
EcoT22I ATGCAT 1 cut(s) 1537
EcoT38I GRGCYC 2 cut(s) 116, 207
ErhI CCWWGG 2 cut(s) 1061, 1187
Esp3I CGTCTC 1 cut(s) 441
FalI AAGNNNNNCTT 2 cut(s) 760, 792
FaqI GGGAC 4 cut(s) 145, 318, 523, 664
FauI CCCGC 2 cut(s) 125, 345
FauNDI CATATG 2 cut(s) 1271, 1519
Fnu4HI GCNGC 5 cut(s) 121, 181, 347, 817, 993
FokI GGATG 4 cut(s) 410, 481, 1581, 1706
FriOI GRGCYC 2 cut(s) 116, 207
Fsp4HI GCNGC 5 cut(s) 121, 181, 347, 817, 993
FspBI CTAG 3 cut(s) 783, 1266, 1786
GluI GCNGC 5 cut(s) 121, 181, 347, 817, 993
GsuI CTGGAG 3 cut(s) 546, 903, 1244
HaeIII GGCC 8 cut(s) 57, 188, 194, 200, 391, 419, 1022, 1192
HapII CCGG 5 cut(s) 110, 146, 341, 501, 820
Hin1I GRCGYC 1 cut(s) 18
HinfI GANTC 4 cut(s) 706, 736, 846, 1284
HpaII CCGG 5 cut(s) 110, 146, 341, 501, 820
HphI GGTGA 2 cut(s) 494, 1754
Hpy166II GTNNAC 2 cut(s) 898, 900
Hpy188III TCNNGA 9 cut(s) 260, 572, 614, 908, 923, 929, 946, 1244, 1481
Hpy8I GTNNAC 2 cut(s) 898, 900
Hpy99I CGWCG 2 cut(s) 177, 180
HpyAV CCTTC 3 cut(s) 112, 286, 409
HpyCH4III ACNGT 4 cut(s) 64, 375, 593, 1477
HpyCH4IV ACGT 5 cut(s) 18, 434, 1130, 1513, 1564
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 346, 816, 1198
HpyF3I CTNAG 4 cut(s) 875, 1194, 1610, 1738
HpySE526I ACGT 5 cut(s) 18, 434, 1130, 1513, 1564
Hsp92I GRCGYC 1 cut(s) 18
KflI GGGWCCC 1 cut(s) 159
LmnI GCTCC 8 cut(s) 112, 119, 128, 313, 343, 527, 691, 1423
Lsp1109I GCAGC 2 cut(s) 803, 1004
LweI GCATC 4 cut(s) 549, 1093, 1559, 1717
MaeI CTAG 3 cut(s) 783, 1266, 1786
MaeII ACGT 5 cut(s) 18, 434, 1130, 1513, 1564
MaeIII GTNAC 4 cut(s) 482, 870, 1043, 1675
MbiI CCGCTC 2 cut(s) 123, 346
MboII GAAGA 8 cut(s) 146, 458, 644, 1063, 1138, 1259, 1517, 1757
MfeI CAATTG 1 cut(s) 355
MflI RGATCY 3 cut(s) 942, 1057, 1582
MhlI GDGCHC 3 cut(s) 116, 207, 764
MlyI GAGTC 1 cut(s) 1293
MmeI TCCRAC 1 cut(s) 150
Mph1103I ATGCAT 1 cut(s) 1537
MseI TTAA 3 cut(s) 564, 579, 1442
MslI CAYNNNNRTG 3 cut(s) 12, 674, 1349
MspA1I CMGCKG 1 cut(s) 283
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 5 cut(s) 110, 146, 341, 501, 820
MspR9I CCNGG 6 cut(s) 71, 111, 146, 501, 882, 1223
MunI CAATTG 1 cut(s) 355
Mva1269I GAATGC 2 cut(s) 216, 955
MvaI CCWGG 3 cut(s) 71, 882, 1223
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 120, 346, 816, 1198
NciI CCSGG 3 cut(s) 111, 146, 501
NdeI CATATG 2 cut(s) 1271, 1519
NlaIV GGNNCC 4 cut(s) 115, 160, 161, 1067
NmeAIII GCCGAG 4 cut(s) 33, 214, 220, 226
NmuCI GTSAC 2 cut(s) 482, 1675
NsiI ATGCAT 1 cut(s) 1537
NspI RCATGY 1 cut(s) 1535
PaeR7I CTCGAG 1 cut(s) 1719
PagI TCATGA 2 cut(s) 922, 1480
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 2 cut(s) 216, 955
PfeI GAWTC 3 cut(s) 706, 736, 846
PkrI GCNGC 5 cut(s) 122, 182, 348, 818, 994
PleI GAGTC 1 cut(s) 1292
PpsI GAGTC 1 cut(s) 1292
Ppu21I YACGTR 1 cut(s) 1514
PpuMI RGGWCCY 1 cut(s) 159
PsiI TTATAA 1 cut(s) 1655
Psp5II RGGWCCY 1 cut(s) 159
Psp6I CCWGG 3 cut(s) 69, 880, 1221
PspGI CCWGG 3 cut(s) 69, 880, 1221
PspN4I GGNNCC 4 cut(s) 115, 160, 161, 1067
PspPI GGNCC 4 cut(s) 99, 159, 1020, 1065
PspPPI RGGWCCY 1 cut(s) 159
PspXI VCTCGAGB 1 cut(s) 1719
PsuI RGATCY 3 cut(s) 942, 1057, 1582
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 4 cut(s) 353, 539, 899, 1516
RsaNI GTAC 4 cut(s) 352, 538, 898, 1515
RseI CAYNNNNRTG 3 cut(s) 12, 674, 1349
SaqAI TTAA 3 cut(s) 564, 579, 1442
SatI GCNGC 5 cut(s) 121, 181, 347, 817, 993
Sau96I GGNCC 4 cut(s) 99, 159, 1020, 1065
SchI GAGTC 1 cut(s) 1293
ScrFI CCNGG 6 cut(s) 71, 111, 146, 501, 882, 1223
SduI GDGCHC 3 cut(s) 116, 207, 764
SfaNI GCATC 4 cut(s) 549, 1093, 1559, 1717
Sfr274I CTCGAG 1 cut(s) 1719
SinI GGWCC 3 cut(s) 99, 159, 1065
SlaI CTCGAG 1 cut(s) 1719
SmiMI CAYNNNNRTG 3 cut(s) 12, 674, 1349
SmlI CTYRAG 3 cut(s) 38, 563, 1719
SmoI CTYRAG 3 cut(s) 38, 563, 1719
SsiI CCGC 8 cut(s) 118, 121, 181, 338, 346, 349, 504, 1434
SspI AATATT 2 cut(s) 601, 1145
SspMI CTAG 3 cut(s) 783, 1266, 1786
StyD4I CCNGG 6 cut(s) 69, 109, 144, 499, 880, 1221
StyI CCWWGG 2 cut(s) 1061, 1187
TaaI ACNGT 4 cut(s) 64, 375, 593, 1477
TaiI ACGT 5 cut(s) 21, 437, 1133, 1516, 1567
TaqI TCGA 3 cut(s) 141, 261, 1720
TatI WGTACW 2 cut(s) 537, 897
TauI GCSGC 3 cut(s) 123, 183, 349
TfiI GAWTC 3 cut(s) 706, 736, 846
Tru1I TTAA 3 cut(s) 564, 579, 1442
Tru9I TTAA 3 cut(s) 564, 579, 1442
TscAI CASTG 3 cut(s) 67, 1035, 1579
TseFI GTSAC 2 cut(s) 482, 1675
TseI GCWGC 2 cut(s) 816, 992
Tsp45I GTSAC 2 cut(s) 482, 1675
TspDTI ATGAA 9 cut(s) 17, 336, 420, 1139, 1320, 1322, 1367, 1424, 1469
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 3 cut(s) 67, 1035, 1579
Vha464I CTTAAG 1 cut(s) 563
VpaK11BI GGWCC 3 cut(s) 99, 159, 1065
XapI RAATTY 5 cut(s) 137, 298, 1250, 1327, 1354
XceI RCATGY 1 cut(s) 1535
XcmI CCANNNNNNNNNTGG 1 cut(s) 1107
XhoI CTCGAG 1 cut(s) 1719
XspI CTAG 3 cut(s) 783, 1266, 1786
ZraI GACGTC 1 cut(s) 19
Zsp2I ATGCAT 1 cut(s) 1537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.