Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006114

Physical Location & Seq

Genomic Coordinates Forward (+)

1678052 .. 1681427

Gene Structure 3376 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1027000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2145 bp

ATGGCGGAGGGCGGTAGCGAGACCGCCACCGGAGCTAAACCTAAACGTAAACCAATCGTATGGCTGGGGATCTTCCTCATCTCCCACAGCTTCTTGTTCAGTGTCGTGTTCTCCGCCGCCGGGGTGGTGGCGCTCTTGCTGCTTCCGGTTCTCGCCAAGAACACTTATATTTCCGAGAATGCCCTCATGCCTGGCTCCGTGGCGTCTATGCTTTCGAATCAGGAGGTTGCAGAGGCGAATAGATTGGTAAACGATTTGATTCATTCGAATTCGAAGACTCTCGGTTCAGCAATAGGAAGTCAAAGAATCATTGCCCAGCATATGTCGAACTTGGGTGCTGAAGTTAGTTTTCACAAGTTCCATCCTCAGTTAAACCAGTTTCACCCGCTCCACTTCTTTTCTAGCCCTGATTCTGGAAGAGTTGAGCAGAACGTCAGTTGTGCATTGCACGGTGTCAATACTGTTGGAATTATTAGAGCACCGCGTGGTGATGGGAAGGAAGCTATTGTCTTGGTTACACCTTTCAATTCAGCTAAAGTTAACCTGAATGAAGCCTTTTCTTTGGGCATTGCATACTCGGTATTTTCCTTGCTCAACAGAGTTCCTTGGCTGGCCAAGGATGTTATATGGCTCGTTCCCGATTCCCAATTTGGAGAGTATGCTGCAGTTTCTGCTTGGCTAAGAGACTATCACACGCCTGTATTTAGTGGACTGGGCACGCTAGATGCAGAAATGTGTCCTGAAAGCAATAATCTTTATGGAATGAATGAGAACCTGTCAACAGAAAGGGCGATTTACGATGGATTTAAACGTGCTGGAACCATGGCTGCAGCTCTTGTCATCAAGGTAGCAGATCGAAGTGAACATTTTGAGGATAGTCTAGGCATCTATGCTGAGGCATCCAATGGGCAGATGCCAAATCTTGACCTCATCAATGTTGTGAATTACTTAGCAGTACATAGGCAAGGTTTGCGGGTAAAGGTGGAGAAAGTTTGGTCTTTACTTGGCTTGAAGTGGCTCAAGACTTTGGGTGAAATATTAGAATCACTAGGACATTTTGCTAGAAGCTTAAATCCCGACTGGAAATTTGGTATCCCAGCTGCTGATTATGTCGAGGGCAGTGCTACACTTGCAAGTTCATTGTACAACCAGGCTTTGGGTATCCCCACTGGTCCTCACGGTGCCTTCCGTGATTACCAAGTTGATGCAATTACGTTGGAAATTTTCCCCCAATTTTCTTCTTTGGAGAACATAGCCAGGCGAAATGACTTACTTCTACGAAGTGCCCGATTAATTGAAGGAGTCATTCGGTCAGTAAACAACCTTCTAGAGAAGTTTCACCAGTCATTTTTTCTGTACCTGTTAACGTCTCCGGCTAAGTTTGTGTCAGTGGGAGTCTACATGATTGCTTTTGCCCTACTAGTTGCCCCACTCCCCTTGGTAGCAGCTTCTCTCTATGTTAATTCTAGTAACTCTGATTCTAGTGTGAAAAAAGGGAAACCTTCTCCCTCGGCCATCATTACTCTCGAATCATGGAAATGGCTATATGCAACAAAGAAAGCTTTTATTGTTCACTTGTGGGGTTCTGTTGTTTCATTACTCCCGTATTTTATCTGTCAAATACCGAGTTGCACCCCAACAACCAGCTTTGCAATCTGGGTTTCGCTTTCAGTTCTCAGCCTTGTGGTCATGTACGTGGTTTTGGCTTCTCCGTCCTCTCATGCCAATGAATCTCAATCTCAAAGAGAGGAATGGCCCATCTTGAAATCAGTGACTCTTTCAGCTGCCTTCACTGGTTTGTGTCTCATGTCGGTCATTAACTTTGCTACAGCAGAGATCGGGGGGTTACTGATGGTACCAATGTGCTTGATGGCTCATCCGTTGAAGCCCGATGTTAAAGCAAGGAGCATAAGAACACTTTCAAGGGCGGTTTGCAATCTCATTCTGGGGTTTATTGGGTTCCCGCCCCTTGCTTTTATAGTGTTGAAAGCTGCATTTGAAGGTACCGGCAGCACTAATGTTGGTGACTTCTGGTCCTGGGTGGAGTCTCTCTGGGCTTGGAACAGTGCTACTTACCTCTACATAGGTATGGTTCACCTGCCATGCTGGGCGTTGTGTATTCATATTTTGTTTCATCGTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003923 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0006464 GO:0006497 GO:0006505 GO:0006506 GO:0006629 GO:0006643 GO:0006644 GO:0006650 GO:0006661 GO:0006664 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009247 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016255 GO:0016787 GO:0019538 GO:0019637 GO:0030176 GO:0031224 GO:0031226 GO:0031227 GO:0031984 GO:0032991 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042175 GO:0042765 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0045017 GO:0046467 GO:0046474 GO:0046486 GO:0046488 GO:0071704 GO:0071944 GO:0090407 GO:0098796 GO:0098827 GO:0140096 GO:1901135 GO:1901137 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00563 ko01100 map00563 map01100

Pfam Domains

Protein Families

Gaa1

Protein Analysis

715

Amino Acids

78.13

Weight (kDa)

8.0

Isoelectric Point (pI)

37.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gaa1	PF04114	150 - 706	8.5e-101	Gaa1-like, GPI transamidase component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19130 AT5G19130
fragaria_vesca	FvH4_6g51172 FvH4_6g51190 FvH4_6g51190 FvH4_6g51190
malus_domestica	MD09G1027000.v1.1 MD17G1027000.v1.1
prunus_persica	Prupe.3G291700_v2.0.a1 Prupe.3G291700_v2.0.a1 Prupe.3G291700_v2.0.a1 Prupe.3G291700_v2.0.a1 Prupe.3G291700_v2.0.a1
pyrus_communis	pycom111g02110 pycom17g02210
rosa_chinensis	RchiOBHm_Chr2g0172791
rosa_laevigata	RLG00000022137
rosa_multiflora	Rmu_sc0002979.1_g000012 Rmu_sc0010744.1_g000001 Rmu_sc0027475.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079510
rosa_rugosa	Rorug02G0561700
rosa_samantha	Rh2AG639400 Rh2BG652800 Rh2CG618600 Rh2DG666500
rosa_wichuraiana	Rw2G052780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2106
Acc36I	ACCTGC	1 cut(s)	2106
Acc65I	GGTACC	2 cut(s)	1855, 2003
AccB1I	GGYRCC	3 cut(s)	1181, 1855, 2003
AccB7I	CCANNNNNTGG	1 cut(s)	1156
AccBSI	CCGCTC	1 cut(s)	388
AccI	GTMKAC	1 cut(s)	1398
AccII	CGCG	1 cut(s)	484
AclWI	GGATC	1 cut(s)	77
AcoI	YGGCCR	2 cut(s)	612, 1512
AcsI	RAATTY	3 cut(s)	268, 1085, 1221
AcuI	CTGAAG	1 cut(s)	360
AcyI	GRCGYC	1 cut(s)	203
AdeI	CACNNNGTG	1 cut(s)	485
AfaI	GTAC	6 cut(s)	957, 1145, 1358, 1694, 1857, 2005
AfiI	CCNNNNNNNGG	3 cut(s)	120, 413, 1156
AgsI	TTSAA	8 cut(s)	526, 1012, 1298, 1765, 1885, 1923, 1987, 2000
AhdI	GACNNNNNGTC	1 cut(s)	2032
AhlI	ACTAGT	1 cut(s)	1420
AjnI	CCWGG	4 cut(s)	190, 1149, 1256, 2036
Alw21I	GWGCWC	1 cut(s)	481
Alw26I	GTCTC	5 cut(s)	14, 678, 1374, 1808, 2052
AlwI	GGATC	1 cut(s)	77
AlwNI	CAGNNNCTG	2 cut(s)	671, 1103
AoxI	GGCC	3 cut(s)	612, 1512, 1754
ApeKI	GCWGC	9 cut(s)	139, 662, 827, 830, 1100, 1445, 1784, 1991, 2010
ApoI	RAATTY	3 cut(s)	268, 1085, 1221
ArsI	GACNNNNNNTTYG	2 cut(s)	1070, 1102
AseI	ATTAAT	1 cut(s)	1292
Asp700I	GAANNNNTTC	1 cut(s)	1918
Asp718I	GGTACC	2 cut(s)	1855, 2003
AspLEI	GCGC	1 cut(s)	133
AspS9I	GGNCC	3 cut(s)	1172, 1755, 2034
AsuC2I	CCSGG	1 cut(s)	121
AsuHPI	GGTGA	6 cut(s)	374, 500, 1043, 1331, 2036, 2087
AsuII	TTCGAA	3 cut(s)	215, 266, 272
AvaII	GGWCC	2 cut(s)	1172, 2034
BaeGI	GKGCMC	2 cut(s)	719, 1288
BaeI	ACNNNNGTAYC	2 cut(s)	1847, 1880
BalI	TGGCCA	1 cut(s)	614
BanI	GGYRCC	3 cut(s)	1181, 1855, 2003
BarI	GAAGNNNNNNTAC	2 cut(s)	1308, 1340
BbsI	GAAGAC	1 cut(s)	281
Bbv12I	GWGCWC	1 cut(s)	481
BbvCI	CCTCAGC	1 cut(s)	894
BbvI	GCAGC	9 cut(s)	126, 649, 814, 842, 1087, 1457, 1771, 1978, 2022
BccI	CCATC	7 cut(s)	369, 485, 794, 1523, 1766, 1846, 1864
BcgI	CGANNNNNNTGC	2 cut(s)	1103, 1137
BciT130I	CCWGG	4 cut(s)	192, 1151, 1258, 2038
BciVI	GTATCC	2 cut(s)	1103, 1172
BcnI	CCSGG	1 cut(s)	121
BcoDI	GTCTC	5 cut(s)	14, 678, 1374, 1808, 2052
BcuI	ACTAGT	1 cut(s)	1420
BfaI	CTAG	9 cut(s)	402, 722, 881, 1049, 1062, 1328, 1421, 1467, 1482
BfmI	CTRYAG	3 cut(s)	663, 828, 1827
BfoI	RGCGCY	1 cut(s)	134
BfuAI	ACCTGC	1 cut(s)	2106
BfuI	GTATCC	2 cut(s)	1103, 1172
Bme1390I	CCNGG	5 cut(s)	121, 192, 1151, 1258, 2038
Bme18I	GGWCC	2 cut(s)	1172, 2034
BmeRI	GACNNNNNGTC	1 cut(s)	2032
BmgT120I	GGNCC	3 cut(s)	1172, 1755, 2034
BmiI	GGNNCC	6 cut(s)	196, 820, 1183, 1857, 1961, 2005
BmrFI	CCNGG	5 cut(s)	121, 192, 1151, 1258, 2038
BmrI	ACTGGG	1 cut(s)	722
BmsI	GCATC	5 cut(s)	715, 894, 903, 908, 1195
BmuI	ACTGGG	1 cut(s)	722
BpiI	GAAGAC	1 cut(s)	281
Bpu10I	CCTNAGC	1 cut(s)	894
Bpu14I	TTCGAA	3 cut(s)	215, 266, 272
BpuEI	CTTGAG	1 cut(s)	1004
BpuMI	CCSGG	1 cut(s)	121
BsaAI	YACGTR	1 cut(s)	1696
BsaHI	GRCGYC	1 cut(s)	203
BsaI	GGTCTC	1 cut(s)	14
BsaJI	CCNNGG	8 cut(s)	120, 198, 605, 615, 822, 1437, 1509, 2037
BsaWI	WCCGGW	2 cut(s)	29, 145
Bsc4I	CCNNNNNNNGG	3 cut(s)	120, 413, 1156
Bse118I	RCCGGY	1 cut(s)	2006
Bse1I	ACTGG	6 cut(s)	376, 717, 1085, 1174, 1342, 1798
Bse3DI	GCAATG	3 cut(s)	309, 443, 567
BseBI	CCWGG	4 cut(s)	192, 1151, 1258, 2038
BseDI	CCNNGG	8 cut(s)	120, 198, 605, 615, 822, 1437, 1509, 2037
BseGI	GGATG	4 cut(s)	361, 625, 899, 1876
BseLI	CCNNNNNNNGG	3 cut(s)	120, 413, 1156
BseMI	GCAATG	3 cut(s)	309, 443, 567
BseMII	CTCAG	3 cut(s)	380, 885, 1690
BseNI	ACTGG	6 cut(s)	376, 717, 1085, 1174, 1342, 1798
BseSI	GKGCMC	2 cut(s)	719, 1288
BseXI	GCAGC	9 cut(s)	126, 649, 814, 842, 1087, 1457, 1771, 1978, 2022
BseYI	CCCAGC	4 cut(s)	64, 315, 1096, 2106
Bsh1236I	CGCG	1 cut(s)	484
BshFI	GGCC	3 cut(s)	614, 1514, 1756
BshNI	GGYRCC	3 cut(s)	1181, 1855, 2003
BsiHKAI	GWGCWC	1 cut(s)	481
BsiSI	CCGG	5 cut(s)	30, 120, 146, 1373, 2007
BslI	CCNNNNNNNGG	3 cut(s)	120, 413, 1156
BsmAI	GTCTC	5 cut(s)	14, 678, 1374, 1808, 2052
BsmBI	CGTCTC	1 cut(s)	1374
BsmI	GAATGC	1 cut(s)	184
BsnI	GGCC	3 cut(s)	614, 1514, 1756
Bso31I	GGTCTC	1 cut(s)	14
Bsp119I	TTCGAA	3 cut(s)	215, 266, 272
Bsp1286I	GDGCHC	3 cut(s)	481, 719, 1288
Bsp1407I	TGTACA	1 cut(s)	1143
Bsp143I	GATC	3 cut(s)	69, 853, 1836
Bsp19I	CCATGG	1 cut(s)	822
BspANI	GGCC	3 cut(s)	614, 1514, 1756
BspCNI	CTCAG	3 cut(s)	379, 886, 1689
BspFNI	CGCG	1 cut(s)	484
BspLI	GGNNCC	6 cut(s)	196, 820, 1183, 1857, 1961, 2005
BspMAI	CTGCAG	2 cut(s)	667, 832
BspMI	ACCTGC	1 cut(s)	2106
BspPI	GGATC	1 cut(s)	77
BspT104I	TTCGAA	3 cut(s)	215, 266, 272
BspT107I	GGYRCC	3 cut(s)	1181, 1855, 2003
BspTNI	GGTCTC	1 cut(s)	14
BsrBI	CCGCTC	1 cut(s)	388
BsrDI	GCAATG	3 cut(s)	309, 443, 567
BsrFI	RCCGGY	1 cut(s)	2006
BsrGI	TGTACA	1 cut(s)	1143
BsrI	ACTGG	6 cut(s)	376, 717, 1085, 1174, 1342, 1798
BssAI	RCCGGY	1 cut(s)	2006
BssECI	CCNNGG	8 cut(s)	120, 198, 605, 615, 822, 1437, 1509, 2037
BssMI	GATC	3 cut(s)	69, 853, 1836
BssNI	GRCGYC	1 cut(s)	203
BssT1I	CCWWGG	4 cut(s)	605, 615, 822, 1437
Bst2UI	CCWGG	4 cut(s)	192, 1151, 1258, 2038
Bst4CI	ACNGT	4 cut(s)	452, 463, 1181, 2066
Bst6I	CTCTTC	1 cut(s)	412
BstACI	GRCGYC	1 cut(s)	203
BstAPI	GCANNNNNTGC	2 cut(s)	671, 970
BstAUI	TGTACA	1 cut(s)	1143
BstBAI	YACGTR	1 cut(s)	1696
BstBI	TTCGAA	3 cut(s)	215, 266, 272
BstC8I	GCNNGC	2 cut(s)	612, 719
BstDEI	CTNAG	6 cut(s)	366, 680, 894, 949, 1377, 1676
BstDSI	CCRYGG	2 cut(s)	198, 822
BstF5I	GGATG	4 cut(s)	361, 625, 899, 1876
BstFNI	CGCG	1 cut(s)	484
BstH2I	RGCGCY	1 cut(s)	134
BstHHI	GCGC	1 cut(s)	133
BstKTI	GATC	3 cut(s)	72, 856, 1839
BstMAI	GTCTC	5 cut(s)	14, 678, 1374, 1808, 2052
BstMBI	GATC	3 cut(s)	69, 853, 1836
BstMWI	GCNNNNNNNGC	5 cut(s)	32, 139, 671, 970, 1130
BstNI	CCWGG	4 cut(s)	192, 1151, 1258, 2038
BstSCI	CCNGG	5 cut(s)	119, 190, 1149, 1256, 2036
BstSFI	CTRYAG	3 cut(s)	663, 828, 1827
BstSLI	GKGCMC	2 cut(s)	719, 1288
BstUI	CGCG	1 cut(s)	484
BstV1I	GCAGC	9 cut(s)	126, 649, 814, 842, 1087, 1457, 1771, 1978, 2022
BstV2I	GAAGAC	1 cut(s)	281
BstX2I	RGATCY	1 cut(s)	69
BstXI	CCANNNNNNTGG	1 cut(s)	60
BstYI	RGATCY	1 cut(s)	69
BsuI	GTATCC	2 cut(s)	1103, 1172
BsuRI	GGCC	3 cut(s)	614, 1514, 1756
BtgI	CCRYGG	2 cut(s)	198, 822
BtsCI	GGATG	4 cut(s)	361, 625, 899, 1876
BtsI	GCAGTG	1 cut(s)	1126
BtsIMutI	CAGTG	7 cut(s)	106, 1126, 1167, 1395, 1776, 1791, 2071
BveI	ACCTGC	1 cut(s)	2106
Cac8I	GCNNGC	2 cut(s)	612, 719
CaiI	CAGNNNCTG	2 cut(s)	671, 1103
CfoI	GCGC	1 cut(s)	133
Cfr10I	RCCGGY	1 cut(s)	2006
Cfr13I	GGNCC	3 cut(s)	1172, 1755, 2034
CseI	GACGC	1 cut(s)	192
Csp6I	GTAC	6 cut(s)	956, 1144, 1357, 1693, 1856, 2004
CspCI	CAANNNNNGTGG	2 cut(s)	1419, 1454
CviAII	CATG	8 cut(s)	187, 823, 1402, 1533, 1690, 1721, 1807, 2103
CviQI	GTAC	6 cut(s)	956, 1144, 1357, 1693, 1856, 2004
DdeI	CTNAG	6 cut(s)	366, 680, 894, 949, 1377, 1676
DpnI	GATC	3 cut(s)	71, 855, 1838
DpnII	GATC	3 cut(s)	69, 853, 1836
DraI	TTTAAA	1 cut(s)	808
DraIII	CACNNNGTG	1 cut(s)	485
DriI	GACNNNNNGTC	1 cut(s)	2032
EaeI	YGGCCR	2 cut(s)	612, 1512
Eam1104I	CTCTTC	1 cut(s)	412
Eam1105I	GACNNNNNGTC	1 cut(s)	2032
EarI	CTCTTC	1 cut(s)	412
EciI	GGCGGA	2 cut(s)	20, 103
Eco130I	CCWWGG	4 cut(s)	605, 615, 822, 1437
Eco31I	GGTCTC	1 cut(s)	14
Eco47I	GGWCC	2 cut(s)	1172, 2034
Eco57I	CTGAAG	1 cut(s)	360
EcoRI	GAATTC	1 cut(s)	268
EcoRII	CCWGG	4 cut(s)	190, 1149, 1256, 2036
EcoT14I	CCWWGG	4 cut(s)	605, 615, 822, 1437
ErhI	CCWWGG	4 cut(s)	605, 615, 822, 1437
Esp3I	CGTCTC	1 cut(s)	1374
FaeI	CATG	8 cut(s)	190, 826, 1405, 1536, 1693, 1724, 1810, 2106
FatI	CATG	8 cut(s)	186, 822, 1401, 1532, 1689, 1720, 1806, 2102
FauI	CCCGC	3 cut(s)	393, 966, 1971
FauNDI	CATATG	1 cut(s)	321
FblI	GTMKAC	1 cut(s)	1398
FokI	GGATG	4 cut(s)	348, 632, 886, 1863
FspBI	CTAG	9 cut(s)	402, 722, 881, 1049, 1062, 1328, 1421, 1467, 1482
GlaI	GCGC	1 cut(s)	132
GsaI	CCCAGC	4 cut(s)	68, 319, 1100, 2110
HaeII	RGCGCY	1 cut(s)	134
HaeIII	GGCC	3 cut(s)	614, 1514, 1756
HapII	CCGG	5 cut(s)	30, 120, 146, 1373, 2007
HgaI	GACGC	1 cut(s)	192
HhaI	GCGC	1 cut(s)	133
Hin1I	GRCGYC	1 cut(s)	203
Hin1II	CATG	8 cut(s)	190, 826, 1405, 1536, 1693, 1724, 1810, 2106
Hin6I	GCGC	1 cut(s)	131
HinP1I	GCGC	1 cut(s)	131
HincII	GTYRAC	3 cut(s)	541, 780, 1365
HindII	GTYRAC	3 cut(s)	541, 780, 1365
HindIII	AAGCTT	2 cut(s)	1066, 1560
HpaI	GTTAAC	2 cut(s)	541, 1365
HpaII	CCGG	5 cut(s)	30, 120, 146, 1373, 2007
HphI	GGTGA	6 cut(s)	374, 500, 1043, 1331, 2036, 2087
Hpy188I	TCNGA	2 cut(s)	175, 1477
HpyAV	CCTTC	7 cut(s)	490, 1195, 1292, 1334, 1512, 1798, 1994
HpyCH4III	ACNGT	4 cut(s)	452, 463, 1181, 2066
HpyCH4IV	ACGT	6 cut(s)	46, 432, 811, 1214, 1367, 1695
HpyF10VI	GCNNNNNNNGC	5 cut(s)	32, 139, 671, 970, 1130
HpyF3I	CTNAG	6 cut(s)	366, 680, 894, 949, 1377, 1676
HpySE526I	ACGT	6 cut(s)	46, 432, 811, 1214, 1367, 1695
Hsp92I	GRCGYC	1 cut(s)	203
Hsp92II	CATG	8 cut(s)	190, 826, 1405, 1536, 1693, 1724, 1810, 2106
HspAI	GCGC	1 cut(s)	131
KpnI	GGTACC	2 cut(s)	1859, 2007
KspAI	GTTAAC	2 cut(s)	541, 1365
Kzo9I	GATC	3 cut(s)	69, 853, 1836
LmnI	GCTCC	4 cut(s)	32, 200, 393, 1905
Lsp1109I	GCAGC	9 cut(s)	126, 649, 814, 842, 1087, 1457, 1771, 1978, 2022
LweI	GCATC	5 cut(s)	715, 894, 903, 908, 1195
MaeI	CTAG	9 cut(s)	402, 722, 881, 1049, 1062, 1328, 1421, 1467, 1482
MaeII	ACGT	6 cut(s)	46, 432, 811, 1214, 1367, 1695
MaeIII	GTNAC	5 cut(s)	514, 1469, 1771, 1845, 2024
MalI	GATC	3 cut(s)	71, 855, 1838
MbiI	CCGCTC	1 cut(s)	388
MboI	GATC	3 cut(s)	69, 853, 1836
MboII	GAAGA	4 cut(s)	64, 286, 429, 1230
MflI	RGATCY	1 cut(s)	69
MhlI	GDGCHC	3 cut(s)	481, 719, 1288
MlsI	TGGCCA	1 cut(s)	614
MluNI	TGGCCA	1 cut(s)	614
MlyI	GAGTC	5 cut(s)	271, 1311, 1404, 1768, 2054
MmeI	TCCRAC	2 cut(s)	445, 1197
Mox20I	TGGCCA	1 cut(s)	614
MroXI	GAANNNNTTC	1 cut(s)	1918
MscI	TGGCCA	1 cut(s)	614
MslI	CAYNNNNRTG	4 cut(s)	1537, 1694, 1725, 2087
Msp20I	TGGCCA	1 cut(s)	614
MspA1I	CMGCKG	2 cut(s)	1100, 1784
MspI	CCGG	5 cut(s)	30, 120, 146, 1373, 2007
MspR9I	CCNGG	5 cut(s)	121, 192, 1151, 1258, 2038
Mva1269I	GAATGC	1 cut(s)	184
MvaI	CCWGG	4 cut(s)	192, 1151, 1258, 2038
MvnI	CGCG	1 cut(s)	484
MwoI	GCNNNNNNNGC	5 cut(s)	32, 139, 671, 970, 1130
NciI	CCSGG	1 cut(s)	121
NcoI	CCATGG	1 cut(s)	822
NdeI	CATATG	1 cut(s)	321
NdeII	GATC	3 cut(s)	69, 853, 1836
NlaIII	CATG	8 cut(s)	190, 826, 1405, 1536, 1693, 1724, 1810, 2106
NlaIV	GGNNCC	6 cut(s)	196, 820, 1183, 1857, 1961, 2005
NmeAIII	GCCGAG	1 cut(s)	1490
NmuCI	GTSAC	2 cut(s)	1771, 2024
NspV	TTCGAA	3 cut(s)	215, 266, 272
PaqCI	CACCTGC	1 cut(s)	2106
PcsI	WCGNNNNNNNCGW	1 cut(s)	1285
PctI	GAATGC	1 cut(s)	184
PdmI	GAANNNNTTC	1 cut(s)	1918
PfeI	GAWTC	9 cut(s)	217, 259, 306, 410, 641, 1043, 1478, 1529, 1730
PflMI	CCANNNNNTGG	1 cut(s)	1156
PleI	GAGTC	5 cut(s)	271, 1310, 1403, 1768, 2053
PpsI	GAGTC	5 cut(s)	271, 1310, 1403, 1768, 2053
Ppu21I	YACGTR	1 cut(s)	1696
PshBI	ATTAAT	1 cut(s)	1292
Psp6I	CCWGG	4 cut(s)	190, 1149, 1256, 2036
PspFI	CCCAGC	4 cut(s)	64, 315, 1096, 2106
PspGI	CCWGG	4 cut(s)	190, 1149, 1256, 2036
PspN4I	GGNNCC	6 cut(s)	196, 820, 1183, 1857, 1961, 2005
PspPI	GGNCC	3 cut(s)	1172, 1755, 2034
PstI	CTGCAG	2 cut(s)	667, 832
PstNI	CAGNNNCTG	2 cut(s)	671, 1103
PsuI	RGATCY	1 cut(s)	69
PvuII	CAGCTG	2 cut(s)	1100, 1784
RsaI	GTAC	6 cut(s)	957, 1145, 1358, 1694, 1857, 2005
RsaNI	GTAC	6 cut(s)	956, 1144, 1357, 1693, 1856, 2004
RseI	CAYNNNNRTG	4 cut(s)	1537, 1694, 1725, 2087
Sau3AI	GATC	3 cut(s)	69, 853, 1836
Sau96I	GGNCC	3 cut(s)	1172, 1755, 2034
SchI	GAGTC	5 cut(s)	271, 1311, 1404, 1768, 2054
ScrFI	CCNGG	5 cut(s)	121, 192, 1151, 1258, 2038
SduI	GDGCHC	3 cut(s)	481, 719, 1288
SfaNI	GCATC	5 cut(s)	715, 894, 903, 908, 1195
SfcI	CTRYAG	3 cut(s)	663, 828, 1827
SfuI	TTCGAA	3 cut(s)	215, 266, 272
SinI	GGWCC	2 cut(s)	1172, 2034
SmiMI	CAYNNNNRTG	4 cut(s)	1537, 1694, 1725, 2087
SmlI	CTYRAG	1 cut(s)	1019
SmoI	CTYRAG	1 cut(s)	1019
SpeI	ACTAGT	1 cut(s)	1420
SspI	AATATT	1 cut(s)	1038
SspMI	CTAG	9 cut(s)	402, 722, 881, 1049, 1062, 1328, 1421, 1467, 1482
StyD4I	CCNGG	5 cut(s)	119, 190, 1149, 1256, 2036
StyI	CCWWGG	4 cut(s)	605, 615, 822, 1437
TaaI	ACNGT	4 cut(s)	452, 463, 1181, 2066
TaiI	ACGT	6 cut(s)	49, 435, 814, 1217, 1370, 1698
TaqI	TCGA	7 cut(s)	215, 266, 272, 326, 856, 1113, 1527
TaqII	GACCGA	2 cut(s)	1299, 1801
TatI	WGTACW	2 cut(s)	955, 1143
TauI	GCSGC	1 cut(s)	119
TfiI	GAWTC	9 cut(s)	217, 259, 306, 410, 641, 1043, 1478, 1529, 1730
TscAI	CASTG	7 cut(s)	106, 1126, 1174, 1395, 1776, 1798, 2071
TseFI	GTSAC	2 cut(s)	1771, 2024
TseI	GCWGC	9 cut(s)	139, 662, 827, 830, 1100, 1445, 1784, 1991, 2010
Tsp45I	GTSAC	2 cut(s)	1771, 2024
TspDTI	ATGAA	8 cut(s)	251, 564, 779, 1128, 1584, 1743, 2111, 2123
TspGWI	ACGGA	4 cut(s)	187, 1178, 1701, 1869
TspRI	CASTG	7 cut(s)	106, 1126, 1174, 1395, 1776, 1798, 2071
Van91I	CCANNNNNTGG	1 cut(s)	1156
VpaK11BI	GGWCC	2 cut(s)	1172, 2034
VspI	ATTAAT	1 cut(s)	1292
XapI	RAATTY	3 cut(s)	268, 1085, 1221
XbaI	TCTAGA	1 cut(s)	1327
XmiI	GTMKAC	1 cut(s)	1398
XmnI	GAANNNNTTC	1 cut(s)	1918
XspI	CTAG	9 cut(s)	402, 722, 881, 1049, 1062, 1328, 1421, 1467, 1482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.