Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0011023

Physical Location & Seq

Genomic Coordinates Forward (+)

1942706 .. 1948636

Gene Structure 5931 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1032100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3570 bp

ATGCCGGAAGGGCGGAGGAGGAGGGGAGGGATCCAGTTTAGCAAACTGTACTCGTTTTCGTGTTTCCGCTCTCCTCTCCCTGAAAGTCATCCCCAAATCGGTGAAAGAGGGTACTCGAGGGTGGTGCATTGCAATGATCCGGATCATCTGGAGGCACTTGAGTTGAGATACAGGGGAAATTATGTTTCGACTACCAAGTACACGGCGGCGAACTTCATTCCGAAGTCTTTGTTTGAGCAGTTTAGGAGGGTTGCAAATATATATTTTCTTGTTGTAGCTTGTGTTTCGTTCAGTCCGTTGGCCCCCTTTAGAGCTGTTAGTGTAGCTGTGCCGTTGATAGTGGTGATCGGAGCTACTATGGCTAAGGAAGCCGTTGAAGATTGGCGGCGAAGAAAGCAGGATATTGAGGCAAACAGTCGAAAGGTTAGAGTTTATGGTAGGAATTGTACATTCTACAAGACCAGATGGAAGAAACTCCGGGTTGGTGATGTTGTGAAGGTGCACAAGGATGAGTACTTTCCTGCGGATCTGCTTTTGCTTTCGTCAAGCTATGAGGATGGGATTTGCTATGTTGATACCATGAACCTTGATGGAGAAACTAATTTAAAATTGAAGCATGCATTGGAGGTGACATCCCATCTTCAAGATGAAGATTCCTTGGAAAAATTTAAAGCGGTGATCAAGTGTGAGGACCCAAATGAAAATCTGTATTCATTTGTTGGGACATTGGTCTATGATGGAAAGCCTTACTCACTTTCCTTACAACAGATGCTCTTAAGAGATTCTAAGCTGAAAAACACTGAATATGTCTATGGTGTCGTCGTCTTTACTGGTCATGACACAAAAGTGATGCAGAATGCAACAGATCCTCCTTCAAAGAGAAGTAAAATCGAGAGGAAAATGGATAAGATAATCTACGTTCTTTTCAGTTCTCTGGTCGTGATAGCCTTTACTGGATCTCTCTTTTTTGGAATCAACACTAGATGGGATATAAGTGGTGGAAATATAAGAAGGTGGTATCTTCGTCCAGATCATTCAACCGTGTTTTATGACCCCAAAAGACCAGCACTTGCGGCTTTTTTCCATTTCTTGACAGCGCTTATGTTGTATGGATATTTAATACCAATATCTTTATATGTATCCATTGAGATTGTGAAGGTATTACAGAGTATTTTCATTAACCAAGACCGGGATATGTATTATGAGGAAATGGATAGGGCGGCTCATGCACGCACGTCTAACCTGAATGAGGAACTTGGCCAGGTTGATATGATACTTTCTGACAAAACAGGTACACTGACATGTAATTCCATGGAGTTCATTAAATGTTCGATAGCAGGCACTGCTTATGGCCATGGTGTGACAGAAGTGGAGAGGGCGCTGGCAAACAGAAAGGACGGAATCGATGGACTGCCTGAAACTGGTGATATATTAGATCATGCTAGTTATAATGTTGACTCTGGAAAGTCAATAAAGGGTTTCAACTTTAGAGACGTACGCATCATGAATGGGCAGTGGGTCAATGAACCTCATTCGGATATCATACAGAAATTCCTTCGGGTTTTAGCAATCTGTCATACAGCCATCCCAGTTGTGGATAAAGCATCAGGAGAAATAACCTATGAAGCTGAGTCACCAGATGAAGCAGCTTTTGTCATAGCTGCCAGGGAGCTTGGGTCTGAGTTTTTTGAAAGGACACAAACAAGCATATCATTGCATGAGTTGGATTCTGAAAGTGGGAGAAAGGTTGATAGGGAATATGAGCTTCTTCACGTCTTGGAGTTCAGCAGTTCTCGCAAAAGAATGTCAGTAATTGTAAGGAGTCCGGAAAATAAATTACTGCTCCTTTGCAAGGGTGCAGACAGTGCAATTTTGGAAAGGCTTGCAAAAGATGGGTGGCAGTTTGAGGATCAGACCAAGGAGCACATTCATAGATATGCTGAAGCAGGTTTACGAACCTTGGTGATTGCATGCCGTGAGCTTGGTGTAGAAGAATTTGAAATATGGGAAAAGGAGTTTGTGAAGGCCAAAGCTTCTGTCACCGAAAGTCGAGATGTACTGGTGGACGGAGTTGCTGACAAGATTGAAAGGGACTTATTTCTACTTGGTGTGACAGCTGTTGAAGACAAACTGCAAACGGGGGTTCCTGAATGTATTAGGAAGCTTGCCCAAGCTGGGATTAAGATATGGGTATTGACGGGGGATAAAATGGAAACTGCAGTTAACATTGGGTATGCTTGCAGTCTACTTAGACAAGATATGAAACGGATTGTCATCAGTCTCGATTCGCCTGACATAAATGCTTTGGAGAAACAAGGGGATAAAGAGGCAGTTGAAAAGGCTTCTCTTGCAAGCATAAGGAAGCAAATTAGAGAAGGTATTTCGCAAATTAATGAAGCTAAAGAAAGTTCGAACCAAGCTAAATCGTTTGGCTTGATAATTGATGGGAAGTCCTTGGAATTTTGTCTCAAGAAGGATGTCGAAAAATCGTTCTTGGAGCTTGCAATTACTTGTGCTTCTGTTATATGCTGCCGCTCTACGCCCAAACAGAAAGCTCTTGTTACGCGATTGGTAAAACTCGGAACAGGTAAAATAACACTGTCTGTTGGTGACGGTGCAAATGACGTTGGCATGCTTCAAGAAGCTGATATTGGAGTTGGCATTAGTGGTGTTGAAGGGATGCAGGCGGTGATGGCAAGTGATTTTGCAATAGCTCAATTTCGTTTTCTGGAGCGTCTCTTGCTTGTTCATGGGCACTGGTGTTATAGGCGCATATCAATGATGATATGCTACTTCTTCTACAAGAACCTTACATTTGGGTTTACTCTGTTTTGGTTTGAGGCCCATGCTTCTTTCTCTGGTCAACCTGCTTACAACGATTGGTACATGTCATTCTACAATGTCTTCTTCACATCGCTTCCCGTAATTGCTCTTGGCGTTTTTGATCAGGATGTTTCAGCACGGCTATGCCTCAAGTATCCCTCTTTATATCTAGAGGGAGTGGAGAATATCCTCTTCAGTTGGACCCGGATACTTGGTTGGATGCTTAACGGTGTTTTGAGCTCCATAATTATCTTCTTCTTCACCACCAACTCCATGATTGGCCAAGCACTCCAAACAGACGGTAAAGTAGTAGACTATGAAGTCCTCGGGGTCACAATGTACTCGTGTGTAGTTTGGGTTGTCAATTGCCAAATGGCACTCTCCATCAACTACTTCACTTGGATCCAGCACTTCTTCATCTGGGGCAGTATCGCCTTCTGGTATGTATTCTTAGTGATCTACGGCTCTGTCTCACCGAGTGTATCCACAACCGCACACAAAGTTCTAGTTGAAGCCTGTGCTCCCAGTCCTCTGTATTGGATGGTCACCCTTCTCGTTACCATGTGCACTTTGATGCCATATTTCTCGTACAGGGCTTTCCAGACACGGTTCAAACCAATGTGTCACGATGTAATACAACAGAAACGATTAAATGGCTCAGACAAGGAGACTTCCGGCGAATTGCCTCTGAGAGTCAGTAGCAAACTGCAGCACCTGAAGCATAGATTGAGGGAAAGAGAGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1190

Amino Acids

134.9

Weight (kDa)

7.11

Isoelectric Point (pI)

39.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	42 - 107	2.2e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	516 - 712	3.8e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	897 - 1147	9.4e-92	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27870 AT3G27870 AT3G27870
fragaria_vesca	FvH4_6g50810
malus_domestica	MD09G1032100.v1.1 MD17G1033800.v1.1
prunus_persica	Prupe.3G285900_v2.0.a1 Prupe.3G285900_v2.0.a1
pyrus_communis	pycom111g02540
rosa_chinensis	RchiOBHm_Chr2g0172201
rosa_laevigata	RLG00000022092
rosa_multiflora	Rmu_ssc0000252.1_g000050
rosa_roxburghii	Rroxscaffold_2G00080090
rosa_rugosa	Rorug02G0557000 Rorug02G0557100 Rorug02G0557200
rosa_samantha	Rh2AG632600 Rh2BG646200 Rh2DG660800
rosa_wichuraiana	Rw2G052370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1449
AasI	GACNNNNNNGTC	1 cut(s)	3143
Acc36I	ACCTGC	2 cut(s)	1937, 2875
AccBSI	CCGCTC	2 cut(s)	69, 2535
AccI	GTMKAC	2 cut(s)	2245, 3135
AccII	CGCG	1 cut(s)	2566
AccIII	TCCGGA	2 cut(s)	139, 1825
AcoI	YGGCCR	3 cut(s)	1258, 1351, 3103
AcsI	RAATTY	4 cut(s)	665, 1550, 1994, 2459
AcuI	CTGAAG	3 cut(s)	1962, 3001, 3560
AdeI	CACNNNGTG	1 cut(s)	3302
AfeI	AGCGCT	1 cut(s)	1098
AfiI	CCNNNNNNNGG	7 cut(s)	98, 1189, 1249, 1421, 1594, 1852, 2175
AflII	CTTAAG	1 cut(s)	775
AflIII	ACRYGT	2 cut(s)	1301, 2886
AjiI	CACGTC	2 cut(s)	1236, 1774
AjnI	CCWGG	2 cut(s)	1260, 1664
AjuI	GAANNNNNNNTTGG	4 cut(s)	605, 637, 2634, 2666
AleI	CACNNNNGTG	1 cut(s)	845
Alw21I	GWGCWC	5 cut(s)	504, 1926, 3065, 3346, 3392
Alw26I	GTCTC	6 cut(s)	1485, 2285, 2471, 2741, 3298, 3485
Alw44I	GTGCAC	2 cut(s)	500, 3388
AlwNI	CAGNNNCTG	2 cut(s)	1343, 3538
Ama87I	CYCGRG	2 cut(s)	115, 3149
Aor13HI	TCCGGA	2 cut(s)	139, 1825
Aor51HI	AGCGCT	1 cut(s)	1098
AoxI	GGCC	6 cut(s)	300, 1258, 1351, 2025, 2841, 3103
ApaLI	GTGCAC	2 cut(s)	500, 3388
ApeKI	GCWGC	4 cut(s)	1646, 1661, 2529, 3532
ApoI	RAATTY	4 cut(s)	665, 1550, 1994, 2459
ArsI	GACNNNNNNTTYG	2 cut(s)	2022, 2054
AseI	ATTAAT	1 cut(s)	2391
AspLEI	GCGC	3 cut(s)	1099, 1381, 2772
AspS9I	GGNCC	4 cut(s)	301, 691, 2842, 3024
AsuC2I	CCSGG	3 cut(s)	479, 1190, 3028
AsuII	TTCGAA	1 cut(s)	2411
AvaI	CYCGRG	2 cut(s)	115, 3149
AvaII	GGWCC	2 cut(s)	691, 3024
BaeGI	GKGCMC	3 cut(s)	504, 2757, 3392
BalI	TGGCCA	3 cut(s)	1260, 1353, 3105
BamHI	GGATCC	2 cut(s)	30, 3225
BanII	GRGCYC	1 cut(s)	3065
BarI	GAAGNNNNNNTAC	2 cut(s)	1935, 1967
BauI	CACGAG	1 cut(s)	3166
BbsI	GAAGAC	2 cut(s)	2130, 2896
Bbv12I	GWGCWC	5 cut(s)	504, 1926, 3065, 3346, 3392
BbvI	GCAGC	4 cut(s)	1648, 1658, 2516, 3544
BceAI	ACGGC	6 cut(s)	219, 316, 356, 1959, 2978, 3301
BcgI	CGANNNNNNTGC	2 cut(s)	106, 140
BciT130I	CCWGG	2 cut(s)	1262, 1666
BciVI	GTATCC	4 cut(s)	1150, 2988, 3024, 3316
BclI	TGATCA	2 cut(s)	678, 2944
BcnI	CCSGG	3 cut(s)	479, 1190, 3028
BcoDI	GTCTC	6 cut(s)	1485, 2285, 2471, 2741, 3298, 3485
BfaI	CTAG	4 cut(s)	981, 1443, 2993, 3329
BfmI	CTRYAG	2 cut(s)	2217, 3530
BfoI	RGCGCY	2 cut(s)	1100, 1382
BfrI	CTTAAG	1 cut(s)	775
BfuAI	ACCTGC	2 cut(s)	1937, 2875
BfuI	GTATCC	4 cut(s)	1150, 2988, 3024, 3316
BglI	GCCNNNNNGGC	1 cut(s)	10
BisI	GCNGC	9 cut(s)	207, 386, 1074, 1221, 1647, 1662, 2530, 2533, 3533
BlsI	GCNGC	9 cut(s)	208, 387, 1075, 1222, 1648, 1663, 2531, 2534, 3534
BmcAI	AGTACT	1 cut(s)	515
Bme1390I	CCNGG	5 cut(s)	479, 1190, 1262, 1666, 3028
Bme18I	GGWCC	2 cut(s)	691, 3024
BmeT110I	CYCGRG	2 cut(s)	115, 3149
BmgBI	CACGTC	2 cut(s)	1236, 1774
BmgT120I	GGNCC	4 cut(s)	301, 691, 2842, 3024
BmiI	GGNNCC	6 cut(s)	32, 303, 693, 2145, 3026, 3227
BmrFI	CCNGG	5 cut(s)	479, 1190, 1262, 1666, 3028
BmrI	ACTGGG	2 cut(s)	1583, 3342
BmsI	GCATC	7 cut(s)	759, 840, 1509, 1613, 2670, 3033, 3387
BmuI	ACTGGG	2 cut(s)	1583, 3342
BoxI	GACNNNNGTC	1 cut(s)	728
BpiI	GAAGAC	2 cut(s)	2130, 2896
BpmI	CTGGAG	2 cut(s)	170, 2750
Bpu10I	CCTNAGC	1 cut(s)	363
Bpu14I	TTCGAA	1 cut(s)	2411
BpuEI	CTTGAG	3 cut(s)	179, 2453, 2957
BpuMI	CCSGG	3 cut(s)	479, 1190, 3028
Bsa29I	ATCGAT	1 cut(s)	1404
BsaBI	GATNNNNATC	2 cut(s)	141, 2273
BsaJI	CCNNGG	8 cut(s)	657, 1311, 1354, 1665, 1917, 1959, 2454, 3148
BsaWI	WCCGGW	2 cut(s)	139, 1825
BsaXI	ACNNNNNCTCC	4 cut(s)	853, 883, 1661, 1691
Bsc4I	CCNNNNNNNGG	7 cut(s)	98, 1189, 1249, 1421, 1594, 1852, 2175
Bse1I	ACTGG	8 cut(s)	34, 835, 958, 1426, 1589, 2064, 2762, 3348
Bse3DI	GCAATG	3 cut(s)	127, 139, 1712
Bse8I	GATNNNNATC	2 cut(s)	141, 2273
BseAI	TCCGGA	2 cut(s)	139, 1825
BseBI	CCWGG	2 cut(s)	1262, 1666
BseCI	ATCGAT	1 cut(s)	1404
BseDI	CCNNGG	8 cut(s)	657, 1311, 1354, 1665, 1917, 1959, 2454, 3148
BseJI	GATNNNNATC	2 cut(s)	141, 2273
BseLI	CCNNNNNNNGG	7 cut(s)	98, 1189, 1249, 1421, 1594, 1852, 2175
BseMI	GCAATG	3 cut(s)	127, 139, 1712
BseMII	CTCAG	4 cut(s)	1620, 1671, 3495, 3503
BseNI	ACTGG	8 cut(s)	34, 835, 958, 1426, 1589, 2064, 2762, 3348
BseRI	GAGGAG	3 cut(s)	31, 34, 63
BseSI	GKGCMC	3 cut(s)	504, 2757, 3392
BseXI	GCAGC	4 cut(s)	1648, 1658, 2516, 3544
BseYI	CCCAGC	1 cut(s)	2174
BsgI	GTGCAG	1 cut(s)	1878
Bsh1236I	CGCG	1 cut(s)	2566
BshFI	GGCC	6 cut(s)	302, 1260, 1353, 2027, 2843, 3105
BshVI	ATCGAT	1 cut(s)	1404
BsiHKAI	GWGCWC	5 cut(s)	504, 1926, 3065, 3346, 3392
BsiHKCI	CYCGRG	2 cut(s)	115, 3149
BsiSI	CCGG	7 cut(s)	5, 140, 478, 1189, 1826, 3028, 3498
BsiWI	CGTACG	1 cut(s)	1495
BslFI	GGGAC	2 cut(s)	736, 2105
BslI	CCNNNNNNNGG	7 cut(s)	98, 1189, 1249, 1421, 1594, 1852, 2175
BsmAI	GTCTC	6 cut(s)	1485, 2285, 2471, 2741, 3298, 3485
BsmBI	CGTCTC	2 cut(s)	1485, 2741
BsmFI	GGGAC	2 cut(s)	736, 2105
BsmI	GAATGC	1 cut(s)	862
BsnI	GGCC	6 cut(s)	302, 1260, 1353, 2027, 2843, 3105
BsoBI	CYCGRG	2 cut(s)	115, 3149
Bsp119I	TTCGAA	1 cut(s)	2411
Bsp1286I	GDGCHC	6 cut(s)	504, 1926, 2757, 3065, 3346, 3392
Bsp13I	TCCGGA	2 cut(s)	139, 1825
Bsp1407I	TGTACA	1 cut(s)	446
Bsp19I	CCATGG	2 cut(s)	1311, 1354
BspANI	GGCC	6 cut(s)	302, 1260, 1353, 2027, 2843, 3105
BspCNI	CTCAG	4 cut(s)	1621, 1672, 3494, 3504
BspDI	ATCGAT	1 cut(s)	1404
BspEI	TCCGGA	2 cut(s)	139, 1825
BspFNI	CGCG	1 cut(s)	2566
BspHI	TCATGA	2 cut(s)	835, 1503
BspLI	GGNNCC	6 cut(s)	32, 303, 693, 2145, 3026, 3227
BspMAI	CTGCAG	2 cut(s)	2221, 3534
BspMI	ACCTGC	2 cut(s)	1937, 2875
BspT104I	TTCGAA	1 cut(s)	2411
BspTI	CTTAAG	1 cut(s)	775
BsrBI	CCGCTC	2 cut(s)	69, 2535
BsrDI	GCAATG	3 cut(s)	127, 139, 1712
BsrGI	TGTACA	1 cut(s)	446
BsrI	ACTGG	8 cut(s)	34, 835, 958, 1426, 1589, 2064, 2762, 3348
BssECI	CCNNGG	8 cut(s)	657, 1311, 1354, 1665, 1917, 1959, 2454, 3148
BssSI	CACGAG	1 cut(s)	3166
BssT1I	CCWWGG	6 cut(s)	657, 1311, 1354, 1917, 1959, 2454
Bst2BI	CACGAG	1 cut(s)	3166
Bst2UI	CCWGG	2 cut(s)	1262, 1666
Bst4CI	ACNGT	9 cut(s)	48, 416, 1042, 1865, 2601, 2615, 3053, 3125, 3432
Bst6I	CTCTTC	1 cut(s)	3020
BstAFI	CTTAAG	1 cut(s)	775
BstAPI	GCANNNNNTGC	2 cut(s)	1343, 1865
BstAUI	TGTACA	1 cut(s)	446
BstBI	TTCGAA	1 cut(s)	2411
BstDEI	CTNAG	8 cut(s)	363, 786, 1629, 1680, 2249, 3274, 3481, 3512
BstDSI	CCRYGG	2 cut(s)	1311, 1354
BstEII	GGTNACC	1 cut(s)	3367
BstENI	CCTNNNNNAGG	2 cut(s)	1247, 1850
BstFNI	CGCG	1 cut(s)	2566
BstH2I	RGCGCY	2 cut(s)	1100, 1382
BstHHI	GCGC	3 cut(s)	1099, 1381, 2772
BstMAI	GTCTC	6 cut(s)	1485, 2285, 2471, 2741, 3298, 3485
BstNI	CCWGG	2 cut(s)	1262, 1666
BstNSI	RCATGY	5 cut(s)	620, 1305, 1974, 2635, 2890
BstPAI	GACNNNNGTC	1 cut(s)	728
BstPI	GGTNACC	1 cut(s)	3367
BstSCI	CCNGG	5 cut(s)	477, 1188, 1260, 1664, 3026
BstSFI	CTRYAG	2 cut(s)	2217, 3530
BstSLI	GKGCMC	3 cut(s)	504, 2757, 3392
BstUI	CGCG	1 cut(s)	2566
BstV1I	GCAGC	4 cut(s)	1648, 1658, 2516, 3544
BstV2I	GAAGAC	2 cut(s)	2130, 2896
BstX2I	RGATCY	5 cut(s)	30, 526, 865, 956, 3225
BstYI	RGATCY	5 cut(s)	30, 526, 865, 956, 3225
Bsu15I	ATCGAT	1 cut(s)	1404
BsuI	GTATCC	4 cut(s)	1150, 2988, 3024, 3316
BsuRI	GGCC	6 cut(s)	302, 1260, 1353, 2027, 2843, 3105
BsuTUI	ATCGAT	1 cut(s)	1404
BtgI	CCRYGG	2 cut(s)	1311, 1354
BtgZI	GCGATG	1 cut(s)	2898
BtrI	CACGTC	2 cut(s)	1236, 1774
BtsI	GCAGTG	2 cut(s)	1341, 1520
BtsIMutI	CAGTG	7 cut(s)	798, 1295, 1341, 1520, 1870, 2597, 2755
BveI	ACCTGC	2 cut(s)	1937, 2875
CaiI	CAGNNNCTG	2 cut(s)	1343, 3538
CciI	TCATGA	2 cut(s)	835, 1503
CfoI	GCGC	3 cut(s)	1099, 1381, 2772
Cfr13I	GGNCC	4 cut(s)	301, 691, 2842, 3024
ClaI	ATCGAT	1 cut(s)	1404
CseI	GACGC	1 cut(s)	2723
DdeI	CTNAG	8 cut(s)	363, 786, 1629, 1680, 2249, 3274, 3481, 3512
DraI	TTTAAA	2 cut(s)	606, 670
DraIII	CACNNNGTG	1 cut(s)	3302
DrdI	GACNNNNNNGTC	1 cut(s)	3143
DseDI	GACNNNNNNGTC	1 cut(s)	3143
EaeI	YGGCCR	3 cut(s)	1258, 1351, 3103
Eam1104I	CTCTTC	1 cut(s)	3020
EarI	CTCTTC	1 cut(s)	3020
EciI	GGCGGA	1 cut(s)	28
Ecl136II	GAGCTC	1 cut(s)	3063
Eco130I	CCWWGG	6 cut(s)	657, 1311, 1354, 1917, 1959, 2454
Eco24I	GRGCYC	1 cut(s)	3065
Eco32I	GATATC	1 cut(s)	1540
Eco47I	GGWCC	2 cut(s)	691, 3024
Eco47III	AGCGCT	1 cut(s)	1098
Eco53kI	GAGCTC	1 cut(s)	3063
Eco57I	CTGAAG	3 cut(s)	1962, 3001, 3560
Eco88I	CYCGRG	2 cut(s)	115, 3149
Eco91I	GGTNACC	1 cut(s)	3367
EcoICRI	GAGCTC	1 cut(s)	3063
EcoNI	CCTNNNNNAGG	2 cut(s)	1247, 1850
EcoO109I	RGGNCCY	1 cut(s)	691
EcoO65I	GGTNACC	1 cut(s)	3367
EcoRII	CCWGG	2 cut(s)	1260, 1664
EcoRV	GATATC	1 cut(s)	1540
EcoT14I	CCWWGG	6 cut(s)	657, 1311, 1354, 1917, 1959, 2454
EcoT22I	ATGCAT	1 cut(s)	622
EcoT38I	GRGCYC	1 cut(s)	3065
ErhI	CCWWGG	6 cut(s)	657, 1311, 1354, 1917, 1959, 2454
Esp3I	CGTCTC	2 cut(s)	1485, 2741
FaqI	GGGAC	2 cut(s)	736, 2105
FbaI	TGATCA	2 cut(s)	678, 2944
FblI	GTMKAC	2 cut(s)	2245, 3135
Fnu4HI	GCNGC	9 cut(s)	207, 386, 1074, 1221, 1647, 1662, 2530, 2533, 3533
FriOI	GRGCYC	1 cut(s)	3065
Fsp4HI	GCNGC	9 cut(s)	207, 386, 1074, 1221, 1647, 1662, 2530, 2533, 3533
FspBI	CTAG	4 cut(s)	981, 1443, 2993, 3329
GlaI	GCGC	3 cut(s)	1098, 1380, 2771
GluI	GCNGC	9 cut(s)	207, 386, 1074, 1221, 1647, 1662, 2530, 2533, 3533
GsaI	CCCAGC	1 cut(s)	2178
GsuI	CTGGAG	2 cut(s)	170, 2750
HaeII	RGCGCY	2 cut(s)	1100, 1382
HaeIII	GGCC	6 cut(s)	302, 1260, 1353, 2027, 2843, 3105
HapII	CCGG	7 cut(s)	5, 140, 478, 1189, 1826, 3028, 3498
HgaI	GACGC	1 cut(s)	2723
HhaI	GCGC	3 cut(s)	1099, 1381, 2772
Hin6I	GCGC	3 cut(s)	1097, 1379, 2770
HinP1I	GCGC	3 cut(s)	1097, 1379, 2770
HincII	GTYRAC	3 cut(s)	1456, 2224, 2864
HindII	GTYRAC	3 cut(s)	1456, 2224, 2864
HindIII	AAGCTT	2 cut(s)	2031, 2162
HpaI	GTTAAC	1 cut(s)	2224
HpaII	CCGG	7 cut(s)	5, 140, 478, 1189, 1826, 3028, 3498
Hpy99I	CGWCG	1 cut(s)	824
HpyCH4III	ACNGT	9 cut(s)	48, 416, 1042, 1865, 2601, 2615, 3053, 3125, 3432
HpyCH4IV	ACGT	5 cut(s)	918, 1235, 1494, 1773, 2625
HpyF3I	CTNAG	8 cut(s)	363, 786, 1629, 1680, 2249, 3274, 3481, 3512
HpySE526I	ACGT	5 cut(s)	918, 1235, 1494, 1773, 2625
HspAI	GCGC	3 cut(s)	1097, 1379, 2770
Kpn2I	TCCGGA	2 cut(s)	139, 1825
Ksp22I	TGATCA	2 cut(s)	678, 2944
KspAI	GTTAAC	1 cut(s)	2224
LmnI	GCTCC	8 cut(s)	350, 1669, 1848, 1921, 2497, 2731, 3068, 3349
Lsp1109I	GCAGC	4 cut(s)	1648, 1658, 2516, 3544
LweI	GCATC	7 cut(s)	759, 840, 1509, 1613, 2670, 3033, 3387
MaeI	CTAG	4 cut(s)	981, 1443, 2993, 3329
MaeII	ACGT	5 cut(s)	918, 1235, 1494, 1773, 2625
MbiI	CCGCTC	2 cut(s)	69, 2535
MfeI	CAATTG	1 cut(s)	3187
MflI	RGATCY	5 cut(s)	30, 526, 865, 956, 3225
MhlI	GDGCHC	6 cut(s)	504, 1926, 2757, 3065, 3346, 3392
MlsI	TGGCCA	3 cut(s)	1260, 1353, 3105
MluNI	TGGCCA	3 cut(s)	1260, 1353, 3105
MlyI	GAGTC	4 cut(s)	1451, 1640, 1831, 3525
MmeI	TCCRAC	3 cut(s)	1704, 3002, 3020
Mox20I	TGGCCA	3 cut(s)	1260, 1353, 3105
Mph1103I	ATGCAT	1 cut(s)	622
MroI	TCCGGA	2 cut(s)	139, 1825
MscI	TGGCCA	3 cut(s)	1260, 1353, 3105
MslI	CAYNNNNRTG	8 cut(s)	132, 507, 845, 1300, 1935, 2777, 2965, 3395
Msp20I	TGGCCA	3 cut(s)	1260, 1353, 3105
MspA1I	CMGCKG	1 cut(s)	2117
MspCI	CTTAAG	1 cut(s)	775
MspI	CCGG	7 cut(s)	5, 140, 478, 1189, 1826, 3028, 3498
MspR9I	CCNGG	5 cut(s)	479, 1190, 1262, 1666, 3028
MunI	CAATTG	1 cut(s)	3187
Mva1269I	GAATGC	1 cut(s)	862
MvaI	CCWGG	2 cut(s)	1262, 1666
MvnI	CGCG	1 cut(s)	2566
NciI	CCSGG	3 cut(s)	479, 1190, 3028
NcoI	CCATGG	2 cut(s)	1311, 1354
NlaIV	GGNNCC	6 cut(s)	32, 303, 693, 2145, 3026, 3227
NmuCI	GTSAC	9 cut(s)	628, 1360, 1632, 2038, 2110, 2609, 3154, 3367, 3446
NsiI	ATGCAT	1 cut(s)	622
NspI	RCATGY	5 cut(s)	620, 1305, 1974, 2635, 2890
NspV	TTCGAA	1 cut(s)	2411
OliI	CACNNNNGTG	1 cut(s)	845
PaeI	GCATGC	3 cut(s)	620, 1974, 2635
PaeR7I	CTCGAG	1 cut(s)	115
PagI	TCATGA	2 cut(s)	835, 1503
PciI	ACATGT	2 cut(s)	1301, 2886
PcsI	WCGNNNNNNNCGW	1 cut(s)	293
PctI	GAATGC	1 cut(s)	862
PfeI	GAWTC	6 cut(s)	653, 782, 972, 1401, 1727, 2285
Pfl23II	CGTACG	1 cut(s)	1495
PkrI	GCNGC	9 cut(s)	208, 387, 1075, 1222, 1648, 1663, 2531, 2534, 3534
PleI	GAGTC	4 cut(s)	1451, 1639, 1830, 3524
PpsI	GAGTC	4 cut(s)	1451, 1639, 1830, 3524
PpuMI	RGGWCCY	1 cut(s)	691
PscI	ACATGT	2 cut(s)	1301, 2886
PshAI	GACNNNNGTC	1 cut(s)	728
PshBI	ATTAAT	1 cut(s)	2391
PsiI	TTATAA	1 cut(s)	1449
Psp124BI	GAGCTC	1 cut(s)	3065
Psp5II	RGGWCCY	1 cut(s)	691
Psp6I	CCWGG	2 cut(s)	1260, 1664
PspEI	GGTNACC	1 cut(s)	3367
PspFI	CCCAGC	1 cut(s)	2174
PspGI	CCWGG	2 cut(s)	1260, 1664
PspLI	CGTACG	1 cut(s)	1495
PspN4I	GGNNCC	6 cut(s)	32, 303, 693, 2145, 3026, 3227
PspPI	GGNCC	4 cut(s)	301, 691, 2842, 3024
PspPPI	RGGWCCY	1 cut(s)	691
PspXI	VCTCGAGB	1 cut(s)	115
PstI	CTGCAG	2 cut(s)	2221, 3534
PstNI	CAGNNNCTG	2 cut(s)	1343, 3538
PsuI	RGATCY	5 cut(s)	30, 526, 865, 956, 3225
PvuII	CAGCTG	1 cut(s)	2117
RseI	CAYNNNNRTG	8 cut(s)	132, 507, 845, 1300, 1935, 2777, 2965, 3395
SacI	GAGCTC	1 cut(s)	3065
SatI	GCNGC	9 cut(s)	207, 386, 1074, 1221, 1647, 1662, 2530, 2533, 3533
Sau96I	GGNCC	4 cut(s)	301, 691, 2842, 3024
ScaI	AGTACT	1 cut(s)	515
SchI	GAGTC	4 cut(s)	1451, 1640, 1831, 3525
ScrFI	CCNGG	5 cut(s)	479, 1190, 1262, 1666, 3028
SduI	GDGCHC	6 cut(s)	504, 1926, 2757, 3065, 3346, 3392
SfaNI	GCATC	7 cut(s)	759, 840, 1509, 1613, 2670, 3033, 3387
SfcI	CTRYAG	2 cut(s)	2217, 3530
Sfr274I	CTCGAG	1 cut(s)	115
SfuI	TTCGAA	1 cut(s)	2411
SinI	GGWCC	2 cut(s)	691, 3024
SlaI	CTCGAG	1 cut(s)	115
SmiMI	CAYNNNNRTG	8 cut(s)	132, 507, 845, 1300, 1935, 2777, 2965, 3395
SmlI	CTYRAG	5 cut(s)	115, 158, 775, 2468, 2972
SmoI	CTYRAG	5 cut(s)	115, 158, 775, 2468, 2972
SphI	GCATGC	3 cut(s)	620, 1974, 2635
SspMI	CTAG	4 cut(s)	981, 1443, 2993, 3329
SstI	GAGCTC	1 cut(s)	3065
StyD4I	CCNGG	5 cut(s)	477, 1188, 1260, 1664, 3026
StyI	CCWWGG	6 cut(s)	657, 1311, 1354, 1917, 1959, 2454
TaaI	ACNGT	9 cut(s)	48, 416, 1042, 1865, 2601, 2615, 3053, 3125, 3432
TaiI	ACGT	5 cut(s)	921, 1238, 1497, 1776, 2628
TatI	WGTACW	6 cut(s)	48, 198, 446, 513, 2056, 3162
TauI	GCSGC	5 cut(s)	209, 388, 1076, 1223, 2535
TfiI	GAWTC	6 cut(s)	653, 782, 972, 1401, 1727, 2285
TscAI	CASTG	7 cut(s)	805, 1302, 1348, 1520, 1870, 2604, 2762
TseFI	GTSAC	9 cut(s)	628, 1360, 1632, 2038, 2110, 2609, 3154, 3367, 3446
TseI	GCWGC	4 cut(s)	1646, 1661, 2529, 3532
Tsp45I	GTSAC	9 cut(s)	628, 1360, 1632, 2038, 2110, 2609, 3154, 3367, 3446
TspGWI	ACGGA	4 cut(s)	285, 1413, 2082, 2281
TspRI	CASTG	7 cut(s)	805, 1302, 1348, 1520, 1870, 2604, 2762
Vha464I	CTTAAG	1 cut(s)	775
VneI	GTGCAC	2 cut(s)	500, 3388
VpaK11BI	GGWCC	2 cut(s)	691, 3024
VspI	ATTAAT	1 cut(s)	2391
XagI	CCTNNNNNAGG	2 cut(s)	1247, 1850
XapI	RAATTY	4 cut(s)	665, 1550, 1994, 2459
XbaI	TCTAGA	1 cut(s)	2992
XceI	RCATGY	5 cut(s)	620, 1305, 1974, 2635, 2890
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1591
XhoI	CTCGAG	1 cut(s)	115
XmiI	GTMKAC	2 cut(s)	2245, 3135
XspI	CTAG	4 cut(s)	981, 1443, 2993, 3329
ZrmI	AGTACT	1 cut(s)	515
Zsp2I	ATGCAT	1 cut(s)	622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.