Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0011023

Physical Location & Seq

Genomic Coordinates Reverse (-)

86560945 .. 86568296

Gene Structure 7352 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG646200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3075 bp

ATGCCGAGAGGGCGAAGGAGGACGATACATTTCAGTAAATTGTACTCGTTTTCGTGTATTCGATCTTCTTTCGAAGATGTTCATGCCAAAATTGGGGATAGGGGGTACTCTAGGGTTGTGTACTGTAATGATCCTGATAATTCCGAGGCGCTGCAGTTGAGGTACAGGGGGAACTATGTTTCGACTACCAAGTATACGGCGGCTAATTTCATTCCAAAGTCTTTGTTTGAGCAGTTTAGGAGGGTGGCAAATATATACTTTCTTGTTGTGGCTTTTGTTTCGTTCAGTCCGTTGGCGCCTTTTAAGGCTGTTAGTGTTCTTATACCGCTATTGGTGGTGATTGGAGCTACTATGGTTAAGGAAGCTATAGAAGATTGGAGGCGAAGAAAACAGGATGTGGAGGCAAACAATCGAAAGGTGAAAGTGTATGGTAGGAATCATACATTCATTGAAACCAGATGGAAGAAACTCAGAGTTGGTGATCTTGTGAAGGTGCACAAGGATGAGTACTTTCCTGCTGATCTGCTTTTGCTTTCATCAAGCTATGAGGATGGCATTTGCTATGTGGAAACCATGAACCTTGATGGAGAAACAAATTTAAAATTGAAGCATGCACTGGAGGCGACATCCCCTCTTCAAGATGAAAATTCCTTGGAAAGATTTAGGGCAGTTATCAAGTGTGAGGACCCAAATGAAAATCTATATTCATTTGTTGGGACTTTGTTCTATAACGGCAGAGAATACCCACTTTCCTTGCAACAGATGCTCCTAAGAGACTCTAAGCTGAAAAATACAGAATATGTCTATGGAGTTGTTGTTTTCACTGGGCATGACACAAAAGTCATGGAAAATGCTACAGATCCTCCTTCAAAGAGAAGTAAAATTGAGAGGAAAATGGATAAGATAATCTACATCCTTTTCAGTACTCTATTTGTGATAGCTTTTGTTGGATCTGTCTTTTTTGGAATTGATACCAAGAAAGATTATAGCGGTGGAAAGATGAGAAGGTGGTATCTTCGTCCAGATGATTCCGCTGTGTATTATGACCCCAAAAGGCCAGAAATTGCAGCTATTTTGCATTTTCTGACAGCTCTTATGTTGTATGGATATCTGATACCAATATCTTTATATGTTTCAATTGAGATGGTGAAGGTATTACAGAGTATTTTCATTAACCGAGATCAAGATATGTACTATGAGGAAACAGATAGGCCAGCTCATGCACGCACCTCTAATCTGAATGAGGAACTTGGCCAGATTGATATGATACTGTCTGACAAAACTGGTACATTGACATGCAACTCCATGGAGTTTATTAAATGTTCGATAGCAGGCACTGCTTATGGCCATGGTAAGACAGAAGTGGAAATGGCACTGGCAAACAGGAGGAACGGAGTGGCTGAAACTGGTCATATATCATCTGATGTGGTAGAGCATAGTACTGGTGAAGCTGATAGCTCTCGAAAGTCAATTAAGGGTTTCAACTTCAGGGACGAGCGCATCATGAATGGGCACTGGGTTAATGAACCTCATTCAGATACCATCCAGAAATTCCTTCGGGTTTTAGCAATCTGTCATACAGCCATACCTGTTGTGGATAAAGAATCAGGAGAAATATCCTATGAAGCTGAGTCACCAGATGAAGCAGCTTTTGTCATAGCTGCCAGGGAGCTCGGCTTTGAGTTTTTTGAAAGGACACAAACGAGCATATCGTTGCATGAGTTAGATTACGAGACTGGAGAAAAGGTTGACAGAGAATATGAGCTTCTTCAAGTCTTAGAGTTCAGTAGTTCTCGCAAAAGAATGTCAGTAATTGTTAAGAGTCCGGAAAACAAATTATTGCTTCTTTGCAAAGGTGCAGACAGTGCAATTTTTGAAAGGCTGGCAAAAGATGGGCGGCAGTTTGAGGATCAAACTAAGGAGCACATTCACAAATATGCTGAAGCAGGTTTACGAACCTTGGTGGTTGCACACCGTGAGCTTGGTCTAGAAGAATATAAAGCATGGGAGCAAAAGTTTTTAAAGGCCAAAGCTTCTCTCACTGAAGGCCGAGATGCATTAATGGATGAGCTTGCTGATAAGATTGAAAGGGAATTAATACTACTTGGTGTGACAGCTGTTGAGGACAAACTGCAAAAGGGGGTTCCTGAGTGTATCAACAAGCTTGCTCAGGCTGGGATTAAGATATGGGTATTGACTGGTGATAAGATGGAAACTGCAGTTAACATAGGGTATGCTTGCAGTTTACTTAGACAAGATATGAAACGGATTGTCATCAGTCTTGATTCGCCAGATATAATTGCCTTGGAAAAACAAGGGAAGAAAGACGCAATTCAGAAGGCCTGTCTTGCAAGCATAAGGAAGCAAATTGAAGAAGGTTTTTCGCAAATTAATGAAGCTAAAAAAGGTTCCAGCTCCACTAAAGCATTTGGCTTAATAATTGATGGAAAGTCCTTGGAATATTCACTTAAGAAGGACCTGGAAAAGTCATTTTTTGAGCTTGCAATTAATTGTGCTTCAGTTATATGCTGCCGCTCTACGCCCAAACAGAAAGCTCTTGTTACGAGACTGGTAAAAAATGAGACTGGTAAAACAACACTTGCTGTTGGTGATGGGGCGAATGATGTTGGCATGCTTCAAGAGGCAGATATTGGAGTTGGCATTAGTGGTGTTGAAGGGATGCAGGCAGTGATGGCAAGTGATTTTGCAATAGCTCAATTTCGTTTTCTGGAGCGTCTCTTGCTTGTACACGGGCATTGGTGTTACAGGCGTATATCAATGATGATATGCTACTTCTTCTACAAGAACATAACATTCGGGTTTACTCTGTTTTGGTTTGAGGCCCATAATTCTTTCTCTGGTCTACCTGCTTATAATGATTGGTACATGTCATTCTACAATGTCTTCTTCACATCACTTCCTGTAATTGCTCTTGGTGTCTTCGATCAGGATGTTTCTGCACGGCTATGCCTGAAGGTATACAATTTAATTCATTTGAAAGATTATTTACACAAACAAGGGAAACAGAATAAATTCACTAACAGAATAAATTCAGTGAAATATACTGACTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1024

Amino Acids

116.57

Weight (kDa)

7.74

Isoelectric Point (pI)

40.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	40 - 105	3.9e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	515 - 713	1.2e-20	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	635 - 780	3.3e-06	haloacid dehalogenase-like hydrolase
PhoLip_ATPase_C	PF16212	897 - 1015	4.9e-49	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27870 AT3G27870 AT3G27870
fragaria_vesca	FvH4_6g50810
malus_domestica	MD09G1032100.v1.1 MD17G1033800.v1.1
prunus_persica	Prupe.3G285900_v2.0.a1 Prupe.3G285900_v2.0.a1
pyrus_communis	pycom111g02540
rosa_chinensis	RchiOBHm_Chr2g0172201
rosa_laevigata	RLG00000022092
rosa_multiflora	Rmu_ssc0000252.1_g000050
rosa_roxburghii	Rroxscaffold_2G00080090
rosa_rugosa	Rorug02G0557000 Rorug02G0557100 Rorug02G0557200
rosa_samantha	Rh2AG632600 Rh2BG646200 Rh2DG660800
rosa_wichuraiana	Rw2G052370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2874
AasI	GACNNNNNNGTC	1 cut(s)	839
Acc36I	ACCTGC	2 cut(s)	1937, 2875
AccB1I	GGYRCC	1 cut(s)	295
AccBSI	CCGCTC	1 cut(s)	2535
AccI	GTMKAC	3 cut(s)	194, 2863, 2979
AccIII	TCCGGA	1 cut(s)	1825
AciI	CCGC	6 cut(s)	200, 326, 990, 1032, 1897, 2533
AclWI	GGATC	4 cut(s)	125, 854, 958, 1917
AcoI	YGGCCR	2 cut(s)	1252, 1345
AcsI	RAATTY	5 cut(s)	595, 646, 1550, 3032, 3049
AcuI	CTGAAG	5 cut(s)	1471, 1962, 2064, 2502, 2993
AcyI	GRCGYC	1 cut(s)	296
AdeI	CACNNNGTG	1 cut(s)	1976
AfiI	CCNNNNNNNGG	1 cut(s)	93
AflII	CTTAAG	1 cut(s)	2468
AflIII	ACRYGT	1 cut(s)	2886
AjnI	CCWGG	2 cut(s)	1664, 2478
Alw21I	GWGCWC	3 cut(s)	498, 1674, 1926
Alw26I	GTCTC	5 cut(s)	768, 1727, 2560, 2576, 2741
Alw44I	GTGCAC	1 cut(s)	494
AlwI	GGATC	4 cut(s)	125, 854, 958, 1917
AlwNI	CAGNNNCTG	1 cut(s)	1337
Aor13HI	TCCGGA	1 cut(s)	1825
AoxI	GGCC	8 cut(s)	1055, 1211, 1252, 1345, 2025, 2047, 2340, 2841
ApaLI	GTGCAC	1 cut(s)	494
ApeKI	GCWGC	5 cut(s)	151, 1067, 1646, 1661, 2529
ApoI	RAATTY	5 cut(s)	595, 646, 1550, 3032, 3049
AseI	ATTAAT	4 cut(s)	2060, 2096, 2391, 2508
Asp700I	GAANNNNTTC	3 cut(s)	78, 3032, 3049
AspLEI	GCGC	3 cut(s)	151, 298, 1500
AspS9I	GGNCC	3 cut(s)	685, 2476, 2842
AsuHPI	GGTGA	8 cut(s)	349, 430, 491, 1159, 1457, 1626, 2213, 2621
AsuII	TTCGAA	1 cut(s)	72
AvaII	GGWCC	2 cut(s)	685, 2476
BaeGI	GKGCMC	2 cut(s)	498, 1515
BalI	TGGCCA	2 cut(s)	1254, 1347
BanI	GGYRCC	1 cut(s)	295
BanII	GRGCYC	1 cut(s)	1674
BarI	GAAGNNNNNNTAC	4 cut(s)	49, 81, 1935, 1967
BbsI	GAAGAC	2 cut(s)	2896, 2932
Bbv12I	GWGCWC	3 cut(s)	498, 1674, 1926
BbvI	GCAGC	5 cut(s)	138, 1079, 1648, 1658, 2516
BceAI	ACGGC	3 cut(s)	213, 748, 2978
BciT130I	CCWGG	2 cut(s)	1666, 2480
BcoDI	GTCTC	5 cut(s)	768, 1727, 2560, 2576, 2741
BfaI	CTAG	2 cut(s)	111, 1988
BfmI	CTRYAG	4 cut(s)	152, 366, 855, 2217
BfoI	RGCGCY	2 cut(s)	152, 299
BfrI	CTTAAG	1 cut(s)	2468
BfuAI	ACCTGC	2 cut(s)	1937, 2875
BglI	GCCNNNNNGGC	1 cut(s)	10
BisI	GCNGC	8 cut(s)	152, 201, 1068, 1647, 1662, 1898, 2530, 2533
BlsI	GCNGC	8 cut(s)	153, 202, 1069, 1648, 1663, 1899, 2531, 2534
BmcAI	AGTACT	3 cut(s)	509, 925, 1441
Bme1390I	CCNGG	2 cut(s)	1666, 2480
Bme18I	GGWCC	2 cut(s)	685, 2476
BmgT120I	GGNCC	3 cut(s)	685, 2476, 2842
BmiI	GGNNCC	4 cut(s)	297, 687, 2145, 2410
BmrFI	CCNGG	2 cut(s)	1666, 2480
BmrI	ACTGGG	2 cut(s)	834, 1525
BmsI	GCATC	4 cut(s)	753, 1509, 2044, 2670
BmuI	ACTGGG	2 cut(s)	834, 1525
BpiI	GAAGAC	2 cut(s)	2896, 2932
BpmI	CTGGAG	3 cut(s)	638, 1758, 2750
Bpu10I	CCTNAGC	1 cut(s)	2169
Bpu14I	TTCGAA	1 cut(s)	72
BsaBI	GATNNNNATC	1 cut(s)	2273
BsaHI	GRCGYC	1 cut(s)	296
BsaJI	CCNNGG	8 cut(s)	144, 651, 1305, 1348, 1665, 1959, 2304, 2454
BsaWI	WCCGGW	1 cut(s)	1825
Bsc4I	CCNNNNNNNGG	1 cut(s)	93
Bse8I	GATNNNNATC	1 cut(s)	2273
BseAI	TCCGGA	1 cut(s)	1825
BseBI	CCWGG	2 cut(s)	1666, 2480
BseDI	CCNNGG	8 cut(s)	144, 651, 1305, 1348, 1665, 1959, 2304, 2454
BseGI	GGATG	9 cut(s)	400, 508, 556, 626, 912, 1542, 2071, 2685, 2956
BseJI	GATNNNNATC	1 cut(s)	2273
BseLI	CCNNNNNNNGG	1 cut(s)	93
BseMII	CTCAG	4 cut(s)	484, 1620, 2139, 2183
BseSI	GKGCMC	2 cut(s)	498, 1515
BseXI	GCAGC	5 cut(s)	138, 1079, 1648, 1658, 2516
BseYI	CCCAGC	1 cut(s)	2174
BsgI	GTGCAG	2 cut(s)	1878, 2943
BshFI	GGCC	8 cut(s)	1057, 1213, 1254, 1347, 2027, 2049, 2342, 2843
BshNI	GGYRCC	1 cut(s)	295
BsiHKAI	GWGCWC	3 cut(s)	498, 1674, 1926
BsiSI	CCGG	1 cut(s)	1826
BslFI	GGGAC	2 cut(s)	730, 1505
BslI	CCNNNNNNNGG	1 cut(s)	93
BsmAI	GTCTC	5 cut(s)	768, 1727, 2560, 2576, 2741
BsmBI	CGTCTC	1 cut(s)	2741
BsmFI	GGGAC	2 cut(s)	730, 1505
BsnI	GGCC	8 cut(s)	1057, 1213, 1254, 1347, 2027, 2049, 2342, 2843
Bsp119I	TTCGAA	1 cut(s)	72
Bsp1286I	GDGCHC	4 cut(s)	498, 1515, 1674, 1926
Bsp13I	TCCGGA	1 cut(s)	1825
Bsp1407I	TGTACA	1 cut(s)	2746
Bsp143I	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
Bsp19I	CCATGG	2 cut(s)	1305, 1348
BspACI	CCGC	6 cut(s)	200, 326, 990, 1032, 1897, 2533
BspANI	GGCC	8 cut(s)	1057, 1213, 1254, 1347, 2027, 2049, 2342, 2843
BspCNI	CTCAG	4 cut(s)	483, 1621, 2140, 2182
BspEI	TCCGGA	1 cut(s)	1825
BspHI	TCATGA	1 cut(s)	1503
BspLI	GGNNCC	4 cut(s)	297, 687, 2145, 2410
BspMAI	CTGCAG	2 cut(s)	156, 2221
BspMI	ACCTGC	2 cut(s)	1937, 2875
BspPI	GGATC	4 cut(s)	125, 854, 958, 1917
BspT104I	TTCGAA	1 cut(s)	72
BspT107I	GGYRCC	1 cut(s)	295
BspTI	CTTAAG	1 cut(s)	2468
BsrBI	CCGCTC	1 cut(s)	2535
BsrGI	TGTACA	1 cut(s)	2746
BssECI	CCNNGG	8 cut(s)	144, 651, 1305, 1348, 1665, 1959, 2304, 2454
BssMI	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
BssNAI	GTATAC	2 cut(s)	195, 2980
BssNI	GRCGYC	1 cut(s)	296
BssT1I	CCWWGG	6 cut(s)	651, 1305, 1348, 1959, 2304, 2454
Bst1107I	GTATAC	2 cut(s)	195, 2980
Bst2UI	CCWGG	2 cut(s)	1666, 2480
Bst4CI	ACNGT	5 cut(s)	125, 1272, 1865, 1976, 3071
Bst6I	CTCTTC	1 cut(s)	639
BstACI	GRCGYC	1 cut(s)	296
BstAFI	CTTAAG	1 cut(s)	2468
BstAPI	GCANNNNNTGC	3 cut(s)	763, 1337, 1865
BstAUI	TGTACA	1 cut(s)	2746
BstBI	TTCGAA	1 cut(s)	72
BstDEI	CTNAG	9 cut(s)	470, 770, 780, 1629, 1777, 1917, 2148, 2169, 2249
BstDSI	CCRYGG	2 cut(s)	1305, 1348
BstF5I	GGATG	9 cut(s)	400, 508, 556, 626, 912, 1542, 2071, 2685, 2956
BstH2I	RGCGCY	2 cut(s)	152, 299
BstHHI	GCGC	3 cut(s)	151, 298, 1500
BstKTI	GATC	9 cut(s)	65, 133, 484, 523, 862, 953, 1183, 1912, 2947
BstMAI	GTCTC	5 cut(s)	768, 1727, 2560, 2576, 2741
BstMBI	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
BstMWI	GCNNNNNNNGC	8 cut(s)	10, 620, 763, 1337, 1865, 2348, 2693, 2740
BstNI	CCWGG	2 cut(s)	1666, 2480
BstNSI	RCATGY	4 cut(s)	614, 1299, 2635, 2890
BstSCI	CCNGG	2 cut(s)	1664, 2478
BstSFI	CTRYAG	4 cut(s)	152, 366, 855, 2217
BstSLI	GKGCMC	2 cut(s)	498, 1515
BstV1I	GCAGC	5 cut(s)	138, 1079, 1648, 1658, 2516
BstV2I	GAAGAC	2 cut(s)	2896, 2932
BstX2I	RGATCY	2 cut(s)	859, 950
BstYI	RGATCY	2 cut(s)	859, 950
BstZ17I	GTATAC	2 cut(s)	195, 2980
BsuRI	GGCC	8 cut(s)	1057, 1213, 1254, 1347, 2027, 2049, 2342, 2843
BtgI	CCRYGG	2 cut(s)	1305, 1348
BtsCI	GGATG	9 cut(s)	400, 508, 556, 626, 912, 1542, 2071, 2685, 2956
BtsI	GCAGTG	2 cut(s)	1335, 2694
BtsIMutI	CAGTG	9 cut(s)	614, 822, 1335, 1373, 1513, 1870, 2040, 2694, 3060
BveI	ACCTGC	2 cut(s)	1937, 2875
CaiI	CAGNNNCTG	1 cut(s)	1337
CciI	TCATGA	1 cut(s)	1503
CfoI	GCGC	3 cut(s)	151, 298, 1500
Cfr13I	GGNCC	3 cut(s)	685, 2476, 2842
CseI	GACGC	2 cut(s)	2336, 2723
CspCI	CAANNNNNGTGG	2 cut(s)	735, 770
DdeI	CTNAG	9 cut(s)	470, 770, 780, 1629, 1777, 1917, 2148, 2169, 2249
DinI	GGCGCC	1 cut(s)	297
DpnI	GATC	9 cut(s)	64, 132, 483, 522, 861, 952, 1182, 1911, 2946
DpnII	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
DraI	TTTAAA	2 cut(s)	600, 2022
DraIII	CACNNNGTG	1 cut(s)	1976
DrdI	GACNNNNNNGTC	1 cut(s)	839
DseDI	GACNNNNNNGTC	1 cut(s)	839
EaeI	YGGCCR	2 cut(s)	1252, 1345
Eam1104I	CTCTTC	1 cut(s)	639
EarI	CTCTTC	1 cut(s)	639
Ecl136II	GAGCTC	1 cut(s)	1672
Eco130I	CCWWGG	6 cut(s)	651, 1305, 1348, 1959, 2304, 2454
Eco147I	AGGCCT	1 cut(s)	2342
Eco24I	GRGCYC	1 cut(s)	1674
Eco32I	GATATC	1 cut(s)	1109
Eco47I	GGWCC	2 cut(s)	685, 2476
Eco53kI	GAGCTC	1 cut(s)	1672
Eco57I	CTGAAG	5 cut(s)	1471, 1962, 2064, 2502, 2993
EcoICRI	GAGCTC	1 cut(s)	1672
EcoO109I	RGGNCCY	2 cut(s)	685, 2476
EcoRII	CCWGG	2 cut(s)	1664, 2478
EcoRV	GATATC	1 cut(s)	1109
EcoT14I	CCWWGG	6 cut(s)	651, 1305, 1348, 1959, 2304, 2454
EcoT22I	ATGCAT	1 cut(s)	2059
EcoT38I	GRGCYC	1 cut(s)	1674
EgeI	GGCGCC	1 cut(s)	297
EheI	GGCGCC	1 cut(s)	297
ErhI	CCWWGG	6 cut(s)	651, 1305, 1348, 1959, 2304, 2454
Esp3I	CGTCTC	1 cut(s)	2741
FaqI	GGGAC	2 cut(s)	730, 1505
FblI	GTMKAC	3 cut(s)	194, 2863, 2979
Fnu4HI	GCNGC	8 cut(s)	152, 201, 1068, 1647, 1662, 1898, 2530, 2533
FokI	GGATG	9 cut(s)	407, 515, 563, 613, 899, 1529, 2078, 2692, 2963
FriOI	GRGCYC	1 cut(s)	1674
Fsp4HI	GCNGC	8 cut(s)	152, 201, 1068, 1647, 1662, 1898, 2530, 2533
FspBI	CTAG	2 cut(s)	111, 1988
GlaI	GCGC	3 cut(s)	150, 297, 1499
GluI	GCNGC	8 cut(s)	152, 201, 1068, 1647, 1662, 1898, 2530, 2533
GsaI	CCCAGC	1 cut(s)	2178
GsuI	CTGGAG	3 cut(s)	638, 1758, 2750
HaeII	RGCGCY	2 cut(s)	152, 299
HaeIII	GGCC	8 cut(s)	1057, 1213, 1254, 1347, 2027, 2049, 2342, 2843
HapII	CCGG	1 cut(s)	1826
HgaI	GACGC	2 cut(s)	2336, 2723
HhaI	GCGC	3 cut(s)	151, 298, 1500
Hin1I	GRCGYC	1 cut(s)	296
Hin6I	GCGC	3 cut(s)	149, 296, 1498
HinP1I	GCGC	3 cut(s)	149, 296, 1498
HincII	GTYRAC	2 cut(s)	1750, 2224
HindII	GTYRAC	2 cut(s)	1750, 2224
HindIII	AAGCTT	2 cut(s)	2031, 2162
HinfI	GANTC	7 cut(s)	436, 776, 1028, 1604, 1631, 1822, 2285
HpaI	GTTAAC	1 cut(s)	2224
HpaII	CCGG	1 cut(s)	1826
HphI	GGTGA	8 cut(s)	349, 430, 491, 1159, 1457, 1626, 2213, 2621
Hpy188I	TCNGA	9 cut(s)	145, 473, 1086, 1113, 1239, 1276, 1423, 1537, 2337
HpyCH4III	ACNGT	5 cut(s)	125, 1272, 1865, 1976, 3071
HpyF10VI	GCNNNNNNNGC	8 cut(s)	10, 620, 763, 1337, 1865, 2348, 2693, 2740
HpyF3I	CTNAG	9 cut(s)	470, 770, 780, 1629, 1777, 1917, 2148, 2169, 2249
Hsp92I	GRCGYC	1 cut(s)	296
HspAI	GCGC	3 cut(s)	149, 296, 1498
KasI	GGCGCC	1 cut(s)	295
Kpn2I	TCCGGA	1 cut(s)	1825
KspAI	GTTAAC	1 cut(s)	2224
Kzo9I	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
LmnI	GCTCC	7 cut(s)	344, 771, 1669, 1921, 2008, 2420, 2731
Lsp1109I	GCAGC	5 cut(s)	138, 1079, 1648, 1658, 2516
LweI	GCATC	4 cut(s)	753, 1509, 2044, 2670
MaeI	CTAG	2 cut(s)	111, 1988
MaeIII	GTNAC	4 cut(s)	1632, 2110, 2560, 2762
MalI	GATC	9 cut(s)	64, 132, 483, 522, 861, 952, 1182, 1911, 2946
MbiI	CCGCTC	1 cut(s)	2535
MboI	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
MfeI	CAATTG	1 cut(s)	1137
MflI	RGATCY	2 cut(s)	859, 950
MhlI	GDGCHC	4 cut(s)	498, 1515, 1674, 1926
MlsI	TGGCCA	2 cut(s)	1254, 1347
MluNI	TGGCCA	2 cut(s)	1254, 1347
Mly113I	GGCGCC	1 cut(s)	296
MlyI	GAGTC	3 cut(s)	770, 1640, 1831
MmeI	TCCRAC	1 cut(s)	928
Mox20I	TGGCCA	2 cut(s)	1254, 1347
Mph1103I	ATGCAT	1 cut(s)	2059
MroI	TCCGGA	1 cut(s)	1825
MroXI	GAANNNNTTC	3 cut(s)	78, 3032, 3049
MscI	TGGCCA	2 cut(s)	1254, 1347
MslI	CAYNNNNRTG	4 cut(s)	501, 1294, 1935, 2965
Msp20I	TGGCCA	2 cut(s)	1254, 1347
MspA1I	CMGCKG	2 cut(s)	1034, 2117
MspCI	CTTAAG	1 cut(s)	2468
MspI	CCGG	1 cut(s)	1826
MspR9I	CCNGG	2 cut(s)	1666, 2480
MunI	CAATTG	1 cut(s)	1137
MvaI	CCWGG	2 cut(s)	1666, 2480
MwoI	GCNNNNNNNGC	8 cut(s)	10, 620, 763, 1337, 1865, 2348, 2693, 2740
NarI	GGCGCC	1 cut(s)	296
NcoI	CCATGG	2 cut(s)	1305, 1348
NdeII	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
NlaIV	GGNNCC	4 cut(s)	297, 687, 2145, 2410
NmeAIII	GCCGAG	3 cut(s)	30, 1653, 2075
NmuCI	GTSAC	2 cut(s)	1632, 2110
NsiI	ATGCAT	1 cut(s)	2059
NspI	RCATGY	4 cut(s)	614, 1299, 2635, 2890
NspV	TTCGAA	1 cut(s)	72
PaeI	GCATGC	2 cut(s)	614, 2635
PagI	TCATGA	1 cut(s)	1503
PceI	AGGCCT	1 cut(s)	2342
PciI	ACATGT	1 cut(s)	2886
PcsI	WCGNNNNNNNCGW	2 cut(s)	287, 1709
PdmI	GAANNNNTTC	3 cut(s)	78, 3032, 3049
PfeI	GAWTC	4 cut(s)	436, 1028, 1604, 2285
PkrI	GCNGC	8 cut(s)	153, 202, 1069, 1648, 1663, 1899, 2531, 2534
PleI	GAGTC	3 cut(s)	770, 1639, 1830
PluTI	GGCGCC	1 cut(s)	299
PpsI	GAGTC	3 cut(s)	770, 1639, 1830
PpuMI	RGGWCCY	2 cut(s)	685, 2476
PscI	ACATGT	1 cut(s)	2886
PshBI	ATTAAT	4 cut(s)	2060, 2096, 2391, 2508
PsiI	TTATAA	1 cut(s)	2874
Psp124BI	GAGCTC	1 cut(s)	1674
Psp5II	RGGWCCY	2 cut(s)	685, 2476
Psp6I	CCWGG	2 cut(s)	1664, 2478
PspFI	CCCAGC	1 cut(s)	2174
PspGI	CCWGG	2 cut(s)	1664, 2478
PspN4I	GGNNCC	4 cut(s)	297, 687, 2145, 2410
PspPI	GGNCC	3 cut(s)	685, 2476, 2842
PspPPI	RGGWCCY	2 cut(s)	685, 2476
PstI	CTGCAG	2 cut(s)	156, 2221
PstNI	CAGNNNCTG	1 cut(s)	1337
PsuI	RGATCY	2 cut(s)	859, 950
PvuII	CAGCTG	1 cut(s)	2117
RseI	CAYNNNNRTG	4 cut(s)	501, 1294, 1935, 2965
SacI	GAGCTC	1 cut(s)	1674
SatI	GCNGC	8 cut(s)	152, 201, 1068, 1647, 1662, 1898, 2530, 2533
Sau3AI	GATC	9 cut(s)	62, 130, 481, 520, 859, 950, 1180, 1909, 2944
Sau96I	GGNCC	3 cut(s)	685, 2476, 2842
ScaI	AGTACT	3 cut(s)	509, 925, 1441
SchI	GAGTC	3 cut(s)	770, 1640, 1831
ScrFI	CCNGG	2 cut(s)	1666, 2480
SduI	GDGCHC	4 cut(s)	498, 1515, 1674, 1926
SfaNI	GCATC	4 cut(s)	753, 1509, 2044, 2670
SfcI	CTRYAG	4 cut(s)	152, 366, 855, 2217
SfoI	GGCGCC	1 cut(s)	297
SfuI	TTCGAA	1 cut(s)	72
SinI	GGWCC	2 cut(s)	685, 2476
SmiMI	CAYNNNNRTG	4 cut(s)	501, 1294, 1935, 2965
SmlI	CTYRAG	1 cut(s)	2468
SmoI	CTYRAG	1 cut(s)	2468
SphI	GCATGC	2 cut(s)	614, 2635
SseBI	AGGCCT	1 cut(s)	2342
SsiI	CCGC	6 cut(s)	200, 326, 990, 1032, 1897, 2533
SspDI	GGCGCC	1 cut(s)	295
SspI	AATATT	1 cut(s)	2462
SspMI	CTAG	2 cut(s)	111, 1988
SstI	GAGCTC	1 cut(s)	1674
StuI	AGGCCT	1 cut(s)	2342
StyD4I	CCNGG	2 cut(s)	1664, 2478
StyI	CCWWGG	6 cut(s)	651, 1305, 1348, 1959, 2304, 2454
TaaI	ACNGT	5 cut(s)	125, 1272, 1865, 1976, 3071
TaqI	TCGA	7 cut(s)	61, 72, 182, 412, 1325, 1462, 2943
TatI	WGTACW	7 cut(s)	42, 120, 507, 923, 1191, 1439, 2746
TauI	GCSGC	3 cut(s)	203, 1900, 2535
TfiI	GAWTC	4 cut(s)	436, 1028, 1604, 2285
TscAI	CASTG	9 cut(s)	621, 829, 1342, 1380, 1520, 1870, 2047, 2694, 3060
TseFI	GTSAC	2 cut(s)	1632, 2110
TseI	GCWGC	5 cut(s)	151, 1067, 1646, 1661, 2529
Tsp45I	GTSAC	2 cut(s)	1632, 2110
TspGWI	ACGGA	3 cut(s)	279, 1407, 2281
TspRI	CASTG	9 cut(s)	621, 829, 1342, 1380, 1520, 1870, 2047, 2694, 3060
Vha464I	CTTAAG	1 cut(s)	2468
VneI	GTGCAC	1 cut(s)	494
VpaK11BI	GGWCC	2 cut(s)	685, 2476
VspI	ATTAAT	4 cut(s)	2060, 2096, 2391, 2508
XapI	RAATTY	5 cut(s)	595, 646, 1550, 3032, 3049
XbaI	TCTAGA	1 cut(s)	1987
XceI	RCATGY	4 cut(s)	614, 1299, 2635, 2890
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1591
XmiI	GTMKAC	3 cut(s)	194, 2863, 2979
XmnI	GAANNNNTTC	3 cut(s)	78, 3032, 3049
XspI	CTAG	2 cut(s)	111, 1988
ZrmI	AGTACT	3 cut(s)	509, 925, 1441
Zsp2I	ATGCAT	1 cut(s)	2059

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.