Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0011023

Physical Location & Seq

Genomic Coordinates Forward (+)

2412802 .. 2419489

Gene Structure 6688 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1033800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3579 bp

ATGACCGAAGGGCGGAGGAGGCGAGGGATACATTTTAGCAAACTGTATTCGTTCTCGTGTATTCGATCTCCTTTCGTTGATAGTCATCCCCAAATCGGGGAAAGAGGGTACTCGAGGGTGGTGCATTGTAATGAGCCGGATTGTCCGGAGGCGCTTCAGTTGAGATACGGGGGAAATTATGTTTCGACTACCAAGTACACGGCAGTGAATTTCATCCCCAAGTCTTTGTTTGAGCAGTTTAGGAGGGTTGCCAATATATATTTTCTTGTTGTAGCTTGTGTCTCGTTTAGTCCGTTGGCACCTTTTAAAGCTGTTAGTGTTCTTGCACCGTTGTTAGTGGTGATTGGAGCTACTATGGCCAAGGAAGCTGTTGAAGATTGGCGGCGAAGAAAACAGGACATTGAGGCGAACAATCGAAAGGTTAGAGTTTATGGTAGGAATTATACATTCTATGAGACCAGATGGAAGAAACTCCGGGTTGGTGATATTGTGAAGGTCCACAAGGATGAGTACTTTCCTGCTGATCTTCTGTTACTTTCGTCAAGCTATGAGGACGGAATTTGCTATGTTGAAACTATGAACCTTGACGGAGAAACTAATTTGAAATTGAAGCATGCATTGGAGGTGACATCTCATCTTCAGGATGAAAAATCCTTGGAAAACTTTAAAGCAGTGATCAAGTGTGAGGACCCAAATGAAAATCTGTATTCATTTGTTGGGACTTTGTTCTATGATGGAAAGCCTTACCCGCTTTCCTTGCAACAGATGCTCTTAAGAGACTCTAAGCTGAAAAACACTGAATATGTCTATGGTGTTGTCGTCTTTACCGGTCACGACACAAAAGTGATGCAGAATGCGACAGATCCTCCTTCAAAGAGAAGCAAAATCGAGAGGAAAATGGATAAGATAATCTACATTCTTTTCAGTACTCTTGTTGTGATAGCCTTTACTGGATCTGTCTTTTTTGGAATCAACACTAGACGAGATATAAGTGGTGGAAAAATGAGAAGGTGGTATCTTCGTCCAGATCATACAACTGTATTTTACGACCCCAAAAGACCAGAACTTGCGGCTTTTTTCCATTTCTTGACTGCCCTTATGTTGTATGGATATTTAATACCAATATCTTTATATGTATCGATTGAGATTGTGAAGGTATTACAGAGTATATTCATTAACCAAGATCGGGAAATGTATTACGAGGAAATGGATAGGCCGGCTCATGCACGCACATCTAATCTGAATGAGGAACTTGGTCAAGTTGATATGATACTTTCTGACAAAACAGGCACACTGACATGTAATTCCATGGAGTTCATTAAATGTTCGATAGCAGGCACTGCGTATGGCCATGGTATAACAGAAGTGGAGAGGGCACTGGCAAACAGAAGGGACAGAGTTGATGGACTGCATGAAACTGGTAATGTATCATCTGATGTTTTAGACAGTGCTAGTTATAATGTTGACTCTGGAAAGTCAATAAAAGGTTTCAACTTTCGAGACGAGCGCATCATGAATGGGCAGTGGGTCAATGAACCTCATTCAGATATCATACAGAAATTTTTTCGGGTTTTAGCAATCTGTCATACTGCCATTCCAGTTGTTGATAAAGCATCAGGAGAAATAACCTATGAAGCAGAGTCACCAGATGAAGCAGCTTTTGTCATAGCTGCCAGGGAGCTTGGGTTTGAGTTTTACGAAAGGACACAAACAAGCATATCATTGCATGAGTTGGATTTTGAAAGTGGGAGGAAGGTTGATAGGGAATATGAGCTTCTTCAAGTCTTGGAGTTCAGCAGTTCTCGCAAAAGGATGTCAGTAATTGTAAGGAGTCCAGAAAATAAATTATTGCTCCTTTCCAAGGGTGCAGACAGTGCGATTCTGGAAAGGCTTGCAAAAGATGGGCGGCAGTTTGAGGATCAGACCAAGGAGCACATTCATAGATATGCTGAAGCAGGTTTACGAACCTTGGTGATTGCATACCGTGAGCTTGGTGTAGAAGAATTTGAAATATGGGCGAAGGAGTTTGTGAAGGCTAAAGCTTCTGTCACCGAAGGTCGAGATGTACTGGTGGACGGAGTTGCTGATAAGATTGAAAGGGACTTATTTCTACTTGGTGTGACGGCTGTTGAAGACAAACTGCAAAAGGGGGTGCCTGAATGTATCAGTAAGCTTGCCGAAGCTGGGATTAAGATATGGGTATTGACGGGGGATAAAATGGAAACTGCAGTTAACATTGGGTATGCTTGCAGTTTACTTAGACAAGATATGAAACGGATTGTCATCAGTCTCGATTCACCAGACATAAATGCCTTGGAGAAACAAGGGGATAAGGAGGCAGTTGGGCAGGCTTCTCTTGAAAGCATAAGGAAGCAAATTGAGGAAGGTATTTCGCAAATTAATGAAGCTAAAGGAACTTCGAACCAACCTAAATCGTTTGGCTTGGTAATTGATGGGAAGTCCTTGGAATTTTGTCTCAAGAAGGATGTCAAAAATTCTTTTTTTGAGCTTGCAATTACTTGTGCTTCTGTTATATGCTGCCGCTCTACGCCCAAACAGAAAGCTCTTGTTACGAGATTGGTAAAACTTGGAACCGGTAAAATAACACTTTCTGTTGGTGACGGTGCAAATGACGTTGGCATGCTTCAAGAAGCTGATATTGGAGTTGGCATTAGTGGTGTTGAGGGGATGCAGGCGGTGATGGCAAGTGATTTTGCAATAGCTCAATTCCGTTTTCTGGAGCGTCTCTTGCTTGTACATGGGCATTGGTGCTATAGGCGCATATCAATGATGATATGCTACTTCTTCTACAAGAACATTACATTTGGGTTTACTCTGTTTTGGTTTGAGGCCCATGCTTCTTTCTCCGGTCAACCTGCTTACAATGACTGGTACATGTCATTCTACAATGTCTTCTTCACATCACTCCCTGTAATTGCTCTTGGCGTCTTCGATCAGGATGTTTCTGCACGGTTCTGCCTCAAGTATCCGTCCTTGTATCTGGAGGGAGTGGAGAATATCCTCTTCAGCTGGCCACGAATACTTGGTTGGATGGTTAATGGCGTTTTGAGCTCCATAATTATCTTCTTCTTCACCACCAATTCCATGATTGGCCAAGCTCTCCGAAAAGATGGTAAAGTAGTTGACTATGAAGTCCTAGGGGTCACAATGTACTCTTGCGTAGTTTGGGTCGTAAATTGCCAAATGGCGCTTTCCATCAACTACTTCACCTGGATCCAGCACTTCTTCATCTGGGGCAGTGTCGCCTTCTGGTATATATTCTTAGTGATATACGGCTCTGTCTCACCAAGCGTATCCACAACAGCACACAGAGTTCTCGTCGAAGCCTGTGCTCCCAGTCCTCTGTTTTGGATGGTCACCCTTCTCGTTACCATCTGCACTTTGCTGCCGTATTTCTCATACAGGGCTTTCCAGACACGGTTCAAACCAATGCGTCACGATGTAATACAACTGGAACGGTTAAATGGCTCAGACAACGAGACTTCCGGTGAATTGCCTCTGAGATTCAGTAGCAAACTGCAGCACCTGAAGCAGAGATTGAGGGAAAGAAGGTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1193

Amino Acids

135.64

Weight (kDa)

7.5

Isoelectric Point (pI)

39.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	41 - 106	3.8e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	519 - 716	1.3e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	900 - 1150	1.5e-92	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27870 AT3G27870 AT3G27870
fragaria_vesca	FvH4_6g50810
malus_domestica	MD09G1032100.v1.1 MD17G1033800.v1.1
prunus_persica	Prupe.3G285900_v2.0.a1 Prupe.3G285900_v2.0.a1
pyrus_communis	pycom111g02540
rosa_chinensis	RchiOBHm_Chr2g0172201
rosa_laevigata	RLG00000022092
rosa_multiflora	Rmu_ssc0000252.1_g000050
rosa_roxburghii	Rroxscaffold_2G00080090
rosa_rugosa	Rorug02G0557000 Rorug02G0557100 Rorug02G0557200
rosa_samantha	Rh2AG632600 Rh2BG646200 Rh2DG660800
rosa_wichuraiana	Rw2G052370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1458
AasI	GACNNNNNNGTC	1 cut(s)	3152
Acc36I	ACCTGC	2 cut(s)	1946, 2884
AccB1I	GGYRCC	2 cut(s)	298, 2152
AccBSI	CCGCTC	1 cut(s)	2544
AccIII	TCCGGA	1 cut(s)	145
AciI	CCGC	7 cut(s)	13, 382, 749, 1070, 1906, 2542, 2696
AclWI	GGATC	5 cut(s)	857, 961, 1926, 3229, 3242
AcoI	YGGCCR	4 cut(s)	357, 1348, 3032, 3112
AcsI	RAATTY	6 cut(s)	208, 558, 1559, 2003, 2468, 2494
AcuI	CTGAAG	5 cut(s)	140, 623, 1971, 3010, 3569
AcyI	GRCGYC	1 cut(s)	2946
AfaI	GTAC	8 cut(s)	110, 197, 512, 928, 2067, 2757, 2894, 3173
AfiI	CCNNNNNNNGG	8 cut(s)	12, 95, 96, 97, 1186, 1861, 2184, 2737
AflII	CTTAAG	1 cut(s)	772
AflIII	ACRYGT	2 cut(s)	1298, 2895
AgeI	ACCGGT	2 cut(s)	827, 2594
AjnI	CCWGG	2 cut(s)	1673, 3230
AjuI	GAANNNNNNNTTGG	6 cut(s)	602, 634, 2643, 2675, 3031, 3063
AleI	CACNNNNGTG	2 cut(s)	203, 842
Alw21I	GWGCWC	3 cut(s)	1935, 3074, 3355
Alw26I	GTCTC	9 cut(s)	286, 449, 771, 1494, 2294, 2480, 2750, 3307, 3494
AlwI	GGATC	5 cut(s)	857, 961, 1926, 3229, 3242
AlwNI	CAGNNNCTG	2 cut(s)	1340, 3547
Ama87I	CYCGRG	1 cut(s)	112
Aor13HI	TCCGGA	1 cut(s)	145
AoxI	GGCC	6 cut(s)	357, 1214, 1348, 2850, 3032, 3112
ApeKI	GCWGC	5 cut(s)	1655, 1670, 2538, 3406, 3541
ApoI	RAATTY	6 cut(s)	208, 558, 1559, 2003, 2468, 2494
AseI	ATTAAT	1 cut(s)	2400
AsiGI	ACCGGT	2 cut(s)	827, 2594
AspA2I	CCTAGG	1 cut(s)	3157
AspLEI	GCGC	4 cut(s)	154, 1509, 2781, 3211
AspS9I	GGNCC	3 cut(s)	496, 688, 2851
AsuC2I	CCSGG	1 cut(s)	476
AsuII	TTCGAA	1 cut(s)	2420
AvaI	CYCGRG	1 cut(s)	112
AvaII	GGWCC	2 cut(s)	496, 688
AvrII	CCTAGG	1 cut(s)	3157
BaeGI	GKGCMC	1 cut(s)	1378
BalI	TGGCCA	4 cut(s)	359, 1350, 3034, 3114
BamHI	GGATCC	1 cut(s)	3234
BanI	GGYRCC	2 cut(s)	298, 2152
BanII	GRGCYC	1 cut(s)	3074
BarI	GAAGNNNNNNTAC	2 cut(s)	1944, 1976
BauI	CACGAG	1 cut(s)	55
BbsI	GAAGAC	3 cut(s)	2139, 2905, 2941
Bbv12I	GWGCWC	3 cut(s)	1935, 3074, 3355
BbvI	GCAGC	5 cut(s)	1657, 1667, 2525, 3393, 3553
BceAI	ACGGC	4 cut(s)	216, 2139, 3310, 3394
BcgI	CGANNNNNNTGC	4 cut(s)	103, 137, 3332, 3366
BciT130I	CCWGG	2 cut(s)	1675, 3232
BciVI	GTATCC	3 cut(s)	21, 2997, 3325
BclI	TGATCA	1 cut(s)	675
BcnI	CCSGG	1 cut(s)	476
BcoDI	GTCTC	9 cut(s)	286, 449, 771, 1494, 2294, 2480, 2750, 3307, 3494
BfaI	CTAG	3 cut(s)	978, 1452, 3158
BfmI	CTRYAG	3 cut(s)	2226, 2773, 3539
BfoI	RGCGCY	2 cut(s)	155, 3212
BfrI	CTTAAG	1 cut(s)	772
BfuAI	ACCTGC	2 cut(s)	1946, 2884
BfuI	GTATCC	3 cut(s)	21, 2997, 3325
BisI	GCNGC	9 cut(s)	383, 1071, 1656, 1671, 1907, 2539, 2542, 3407, 3542
BlnI	CCTAGG	1 cut(s)	3157
BlsI	GCNGC	9 cut(s)	384, 1072, 1657, 1672, 1908, 2540, 2543, 3408, 3543
BmcAI	AGTACT	2 cut(s)	512, 928
Bme1390I	CCNGG	3 cut(s)	476, 1675, 3232
Bme18I	GGWCC	2 cut(s)	496, 688
BmeT110I	CYCGRG	1 cut(s)	112
BmgT120I	GGNCC	3 cut(s)	496, 688, 2851
BmiI	GGNNCC	5 cut(s)	300, 690, 2154, 2593, 3236
BmrFI	CCNGG	3 cut(s)	476, 1675, 3232
BmrI	ACTGGG	1 cut(s)	3351
BmsI	GCATC	5 cut(s)	756, 837, 1518, 1622, 2679
BmuI	ACTGGG	1 cut(s)	3351
BpiI	GAAGAC	3 cut(s)	2139, 2905, 2941
BpmI	CTGGAG	2 cut(s)	2759, 3023
Bpu14I	TTCGAA	1 cut(s)	2420
BpuEI	CTTGAG	2 cut(s)	2462, 2966
BpuMI	CCSGG	1 cut(s)	476
Bsa29I	ATCGAT	1 cut(s)	1139
BsaBI	GATNNNNATC	2 cut(s)	84, 2282
BsaHI	GRCGYC	1 cut(s)	2946
BsaI	GGTCTC	1 cut(s)	449
BsaWI	WCCGGW	5 cut(s)	145, 827, 2594, 2867, 3506
BsaXI	ACNNNNNCTCC	4 cut(s)	850, 880, 1670, 1700
Bsc4I	CCNNNNNNNGG	8 cut(s)	12, 95, 96, 97, 1186, 1861, 2184, 2737
Bse118I	RCCGGY	3 cut(s)	827, 1216, 2594
Bse1I	ACTGG	8 cut(s)	955, 1383, 1423, 1598, 2073, 2894, 3357, 3477
Bse3DI	GCAATG	1 cut(s)	1721
Bse8I	GATNNNNATC	2 cut(s)	84, 2282
BseAI	TCCGGA	1 cut(s)	145
BseBI	CCWGG	2 cut(s)	1675, 3232
BseCI	ATCGAT	1 cut(s)	1139
BseJI	GATNNNNATC	2 cut(s)	84, 2282
BseLI	CCNNNNNNNGG	8 cut(s)	12, 95, 96, 97, 1186, 1861, 2184, 2737
BseMI	GCAATG	1 cut(s)	1721
BseMII	CTCAG	2 cut(s)	3504, 3512
BseNI	ACTGG	8 cut(s)	955, 1383, 1423, 1598, 2073, 2894, 3357, 3477
BseRI	GAGGAG	1 cut(s)	31
BseSI	GKGCMC	1 cut(s)	1378
BseXI	GCAGC	5 cut(s)	1657, 1667, 2525, 3393, 3553
BseYI	CCCAGC	1 cut(s)	2183
BsgI	GTGCAG	3 cut(s)	1887, 2952, 3382
BshFI	GGCC	6 cut(s)	359, 1216, 1350, 2852, 3034, 3114
BshNI	GGYRCC	2 cut(s)	298, 2152
BshTI	ACCGGT	2 cut(s)	827, 2594
BshVI	ATCGAT	1 cut(s)	1139
BsiHKAI	GWGCWC	3 cut(s)	1935, 3074, 3355
BsiHKCI	CYCGRG	1 cut(s)	112
BsiSI	CCGG	8 cut(s)	137, 146, 475, 828, 1217, 2595, 2868, 3507
BslFI	GGGAC	3 cut(s)	733, 1406, 2114
BslI	CCNNNNNNNGG	8 cut(s)	12, 95, 96, 97, 1186, 1861, 2184, 2737
BsmAI	GTCTC	9 cut(s)	286, 449, 771, 1494, 2294, 2480, 2750, 3307, 3494
BsmBI	CGTCTC	2 cut(s)	1494, 2750
BsmFI	GGGAC	3 cut(s)	733, 1406, 2114
BsmI	GAATGC	1 cut(s)	859
BsnI	GGCC	6 cut(s)	359, 1216, 1350, 2852, 3034, 3114
Bso31I	GGTCTC	1 cut(s)	449
BsoBI	CYCGRG	1 cut(s)	112
Bsp119I	TTCGAA	1 cut(s)	2420
Bsp1286I	GDGCHC	4 cut(s)	1378, 1935, 3074, 3355
Bsp13I	TCCGGA	1 cut(s)	145
Bsp1407I	TGTACA	1 cut(s)	2755
Bsp19I	CCATGG	2 cut(s)	1308, 1351
BspACI	CCGC	7 cut(s)	13, 382, 749, 1070, 1906, 2542, 2696
BspANI	GGCC	6 cut(s)	359, 1216, 1350, 2852, 3034, 3114
BspCNI	CTCAG	2 cut(s)	3503, 3513
BspDI	ATCGAT	1 cut(s)	1139
BspEI	TCCGGA	1 cut(s)	145
BspHI	TCATGA	1 cut(s)	1512
BspLI	GGNNCC	5 cut(s)	300, 690, 2154, 2593, 3236
BspMAI	CTGCAG	2 cut(s)	2230, 3543
BspMI	ACCTGC	2 cut(s)	1946, 2884
BspPI	GGATC	5 cut(s)	857, 961, 1926, 3229, 3242
BspT104I	TTCGAA	1 cut(s)	2420
BspT107I	GGYRCC	2 cut(s)	298, 2152
BspTI	CTTAAG	1 cut(s)	772
BspTNI	GGTCTC	1 cut(s)	449
BsrBI	CCGCTC	1 cut(s)	2544
BsrDI	GCAATG	1 cut(s)	1721
BsrFI	RCCGGY	3 cut(s)	827, 1216, 2594
BsrGI	TGTACA	1 cut(s)	2755
BsrI	ACTGG	8 cut(s)	955, 1383, 1423, 1598, 2073, 2894, 3357, 3477
BssAI	RCCGGY	3 cut(s)	827, 1216, 2594
BssNI	GRCGYC	1 cut(s)	2946
BssSI	CACGAG	1 cut(s)	55
Bst2BI	CACGAG	1 cut(s)	55
Bst2UI	CCWGG	2 cut(s)	1675, 3232
Bst6I	CTCTTC	1 cut(s)	3029
BstACI	GRCGYC	1 cut(s)	2946
BstAFI	CTTAAG	1 cut(s)	772
BstAPI	GCANNNNNTGC	3 cut(s)	766, 1340, 1874
BstAUI	TGTACA	1 cut(s)	2755
BstBI	TTCGAA	1 cut(s)	2420
BstDEI	CTNAG	5 cut(s)	783, 2258, 3283, 3490, 3521
BstDSI	CCRYGG	2 cut(s)	1308, 1351
BstEII	GGTNACC	1 cut(s)	3376
BstENI	CCTNNNNNAGG	1 cut(s)	1859
BstH2I	RGCGCY	2 cut(s)	155, 3212
BstHHI	GCGC	4 cut(s)	154, 1509, 2781, 3211
BstMAI	GTCTC	9 cut(s)	286, 449, 771, 1494, 2294, 2480, 2750, 3307, 3494
BstNI	CCWGG	2 cut(s)	1675, 3232
BstNSI	RCATGY	4 cut(s)	617, 1302, 2644, 2899
BstPI	GGTNACC	1 cut(s)	3376
BstSCI	CCNGG	3 cut(s)	474, 1673, 3230
BstSFI	CTRYAG	3 cut(s)	2226, 2773, 3539
BstSLI	GKGCMC	1 cut(s)	1378
BstV1I	GCAGC	5 cut(s)	1657, 1667, 2525, 3393, 3553
BstV2I	GAAGAC	3 cut(s)	2139, 2905, 2941
BstX2I	RGATCY	3 cut(s)	862, 953, 3234
BstYI	RGATCY	3 cut(s)	862, 953, 3234
Bsu15I	ATCGAT	1 cut(s)	1139
BsuI	GTATCC	3 cut(s)	21, 2997, 3325
BsuRI	GGCC	6 cut(s)	359, 1216, 1350, 2852, 3034, 3114
BsuTUI	ATCGAT	1 cut(s)	1139
BtgI	CCRYGG	2 cut(s)	1308, 1351
BtsI	GCAGTG	5 cut(s)	210, 678, 1338, 1529, 3265
BveI	ACCTGC	2 cut(s)	1946, 2884
CaiI	CAGNNNCTG	2 cut(s)	1340, 3547
CciI	TCATGA	1 cut(s)	1512
CfoI	GCGC	4 cut(s)	154, 1509, 2781, 3211
Cfr10I	RCCGGY	3 cut(s)	827, 1216, 2594
Cfr13I	GGNCC	3 cut(s)	496, 688, 2851
ClaI	ATCGAT	1 cut(s)	1139
CseI	GACGC	3 cut(s)	2732, 2935, 3443
Csp6I	GTAC	8 cut(s)	109, 196, 511, 927, 2066, 2756, 2893, 3172
CspAI	ACCGGT	2 cut(s)	827, 2594
CviQI	GTAC	8 cut(s)	109, 196, 511, 927, 2066, 2756, 2893, 3172
DdeI	CTNAG	5 cut(s)	783, 2258, 3283, 3490, 3521
DraI	TTTAAA	2 cut(s)	307, 667
DrdI	GACNNNNNNGTC	1 cut(s)	3152
DseDI	GACNNNNNNGTC	1 cut(s)	3152
EaeI	YGGCCR	4 cut(s)	357, 1348, 3032, 3112
Eam1104I	CTCTTC	1 cut(s)	3029
EarI	CTCTTC	1 cut(s)	3029
EciI	GGCGGA	1 cut(s)	28
Ecl136II	GAGCTC	1 cut(s)	3072
Eco24I	GRGCYC	1 cut(s)	3074
Eco31I	GGTCTC	1 cut(s)	449
Eco32I	GATATC	1 cut(s)	1549
Eco47I	GGWCC	2 cut(s)	496, 688
Eco53kI	GAGCTC	1 cut(s)	3072
Eco57I	CTGAAG	5 cut(s)	140, 623, 1971, 3010, 3569
Eco88I	CYCGRG	1 cut(s)	112
Eco91I	GGTNACC	1 cut(s)	3376
EcoICRI	GAGCTC	1 cut(s)	3072
EcoNI	CCTNNNNNAGG	1 cut(s)	1859
EcoO109I	RGGNCCY	1 cut(s)	688
EcoO65I	GGTNACC	1 cut(s)	3376
EcoRII	CCWGG	2 cut(s)	1673, 3230
EcoRV	GATATC	1 cut(s)	1549
EcoT22I	ATGCAT	1 cut(s)	619
EcoT38I	GRGCYC	1 cut(s)	3074
Esp3I	CGTCTC	2 cut(s)	1494, 2750
FaqI	GGGAC	3 cut(s)	733, 1406, 2114
FauI	CCCGC	1 cut(s)	756
FbaI	TGATCA	1 cut(s)	675
Fnu4HI	GCNGC	9 cut(s)	383, 1071, 1656, 1671, 1907, 2539, 2542, 3407, 3542
FriOI	GRGCYC	1 cut(s)	3074
Fsp4HI	GCNGC	9 cut(s)	383, 1071, 1656, 1671, 1907, 2539, 2542, 3407, 3542
FspBI	CTAG	3 cut(s)	978, 1452, 3158
GlaI	GCGC	4 cut(s)	153, 1508, 2780, 3210
GluI	GCNGC	9 cut(s)	383, 1071, 1656, 1671, 1907, 2539, 2542, 3407, 3542
GsaI	CCCAGC	1 cut(s)	2187
GsuI	CTGGAG	2 cut(s)	2759, 3023
HaeII	RGCGCY	2 cut(s)	155, 3212
HaeIII	GGCC	6 cut(s)	359, 1216, 1350, 2852, 3034, 3114
HapII	CCGG	8 cut(s)	137, 146, 475, 828, 1217, 2595, 2868, 3507
HgaI	GACGC	3 cut(s)	2732, 2935, 3443
HhaI	GCGC	4 cut(s)	154, 1509, 2781, 3211
Hin1I	GRCGYC	1 cut(s)	2946
Hin6I	GCGC	4 cut(s)	152, 1507, 2779, 3209
HinP1I	GCGC	4 cut(s)	152, 1507, 2779, 3209
HincII	GTYRAC	4 cut(s)	1465, 2233, 2873, 3145
HindII	GTYRAC	4 cut(s)	1465, 2233, 2873, 3145
HindIII	AAGCTT	2 cut(s)	2040, 2171
HinfI	GANTC	8 cut(s)	779, 969, 1466, 1640, 1831, 1879, 2294, 3525
HpaI	GTTAAC	1 cut(s)	2233
HpaII	CCGG	8 cut(s)	137, 146, 475, 828, 1217, 2595, 2868, 3507
Hpy188I	TCNGA	8 cut(s)	1242, 1279, 1435, 1546, 1923, 3125, 3493, 3522
Hpy99I	CGWCG	1 cut(s)	3344
HpyCH4IV	ACGT	1 cut(s)	2634
HpyF3I	CTNAG	5 cut(s)	783, 2258, 3283, 3490, 3521
HpySE526I	ACGT	1 cut(s)	2634
Hsp92I	GRCGYC	1 cut(s)	2946
HspAI	GCGC	4 cut(s)	152, 1507, 2779, 3209
Kpn2I	TCCGGA	1 cut(s)	145
KroI	GCCGGC	1 cut(s)	1216
KroNI	GCCGGC	1 cut(s)	1218
Ksp22I	TGATCA	1 cut(s)	675
KspAI	GTTAAC	1 cut(s)	2233
LmnI	GCTCC	7 cut(s)	347, 1678, 1857, 1930, 2740, 3077, 3358
Lsp1109I	GCAGC	5 cut(s)	1657, 1667, 2525, 3393, 3553
LweI	GCATC	5 cut(s)	756, 837, 1518, 1622, 2679
MaeI	CTAG	3 cut(s)	978, 1452, 3158
MaeII	ACGT	1 cut(s)	2634
MbiI	CCGCTC	1 cut(s)	2544
MflI	RGATCY	3 cut(s)	862, 953, 3234
MhlI	GDGCHC	4 cut(s)	1378, 1935, 3074, 3355
MlsI	TGGCCA	4 cut(s)	359, 1350, 3034, 3114
MluNI	TGGCCA	4 cut(s)	359, 1350, 3034, 3114
MlyI	GAGTC	4 cut(s)	773, 1460, 1649, 1840
MmeI	TCCRAC	2 cut(s)	1713, 3029
Mox20I	TGGCCA	4 cut(s)	359, 1350, 3034, 3114
Mph1103I	ATGCAT	1 cut(s)	619
MroI	TCCGGA	1 cut(s)	145
MroNI	GCCGGC	1 cut(s)	1216
MscI	TGGCCA	4 cut(s)	359, 1350, 3034, 3114
MslI	CAYNNNNRTG	7 cut(s)	129, 203, 504, 842, 1297, 1944, 2786
Msp20I	TGGCCA	4 cut(s)	359, 1350, 3034, 3114
MspA1I	CMGCKG	1 cut(s)	3030
MspCI	CTTAAG	1 cut(s)	772
MspI	CCGG	8 cut(s)	137, 146, 475, 828, 1217, 2595, 2868, 3507
MspR9I	CCNGG	3 cut(s)	476, 1675, 3232
Mva1269I	GAATGC	1 cut(s)	859
MvaI	CCWGG	2 cut(s)	1675, 3232
NaeI	GCCGGC	1 cut(s)	1218
NciI	CCSGG	1 cut(s)	476
NcoI	CCATGG	2 cut(s)	1308, 1351
NgoMIV	GCCGGC	1 cut(s)	1216
NlaIV	GGNNCC	5 cut(s)	300, 690, 2154, 2593, 3236
NmuCI	GTSAC	9 cut(s)	625, 830, 1641, 2047, 2119, 2618, 3163, 3376, 3455
NsiI	ATGCAT	1 cut(s)	619
NspI	RCATGY	4 cut(s)	617, 1302, 2644, 2899
NspV	TTCGAA	1 cut(s)	2420
OliI	CACNNNNGTG	2 cut(s)	203, 842
PaeI	GCATGC	2 cut(s)	617, 2644
PaeR7I	CTCGAG	1 cut(s)	112
PagI	TCATGA	1 cut(s)	1512
PciI	ACATGT	2 cut(s)	1298, 2895
PcsI	WCGNNNNNNNCGW	1 cut(s)	290
PctI	GAATGC	1 cut(s)	859
PdiI	GCCGGC	1 cut(s)	1218
PfeI	GAWTC	4 cut(s)	969, 1879, 2294, 3525
PinAI	ACCGGT	2 cut(s)	827, 2594
PkrI	GCNGC	9 cut(s)	384, 1072, 1657, 1672, 1908, 2540, 2543, 3408, 3543
PleI	GAGTC	4 cut(s)	773, 1460, 1648, 1839
PpsI	GAGTC	4 cut(s)	773, 1460, 1648, 1839
PpuMI	RGGWCCY	1 cut(s)	688
PscI	ACATGT	2 cut(s)	1298, 2895
PshBI	ATTAAT	1 cut(s)	2400
PsiI	TTATAA	1 cut(s)	1458
Psp124BI	GAGCTC	1 cut(s)	3074
Psp5II	RGGWCCY	1 cut(s)	688
Psp6I	CCWGG	2 cut(s)	1673, 3230
PspEI	GGTNACC	1 cut(s)	3376
PspFI	CCCAGC	1 cut(s)	2183
PspGI	CCWGG	2 cut(s)	1673, 3230
PspN4I	GGNNCC	5 cut(s)	300, 690, 2154, 2593, 3236
PspPI	GGNCC	3 cut(s)	496, 688, 2851
PspPPI	RGGWCCY	1 cut(s)	688
PspXI	VCTCGAGB	1 cut(s)	112
PstI	CTGCAG	2 cut(s)	2230, 3543
PstNI	CAGNNNCTG	2 cut(s)	1340, 3547
PsuI	RGATCY	3 cut(s)	862, 953, 3234
PvuII	CAGCTG	1 cut(s)	3030
RsaI	GTAC	8 cut(s)	110, 197, 512, 928, 2067, 2757, 2894, 3173
RsaNI	GTAC	8 cut(s)	109, 196, 511, 927, 2066, 2756, 2893, 3172
RseI	CAYNNNNRTG	7 cut(s)	129, 203, 504, 842, 1297, 1944, 2786
SacI	GAGCTC	1 cut(s)	3074
SatI	GCNGC	9 cut(s)	383, 1071, 1656, 1671, 1907, 2539, 2542, 3407, 3542
Sau96I	GGNCC	3 cut(s)	496, 688, 2851
ScaI	AGTACT	2 cut(s)	512, 928
SchI	GAGTC	4 cut(s)	773, 1460, 1649, 1840
ScrFI	CCNGG	3 cut(s)	476, 1675, 3232
SduI	GDGCHC	4 cut(s)	1378, 1935, 3074, 3355
SfaNI	GCATC	5 cut(s)	756, 837, 1518, 1622, 2679
SfcI	CTRYAG	3 cut(s)	2226, 2773, 3539
Sfr274I	CTCGAG	1 cut(s)	112
SfuI	TTCGAA	1 cut(s)	2420
SinI	GGWCC	2 cut(s)	496, 688
SlaI	CTCGAG	1 cut(s)	112
SmiMI	CAYNNNNRTG	7 cut(s)	129, 203, 504, 842, 1297, 1944, 2786
SmlI	CTYRAG	4 cut(s)	112, 772, 2477, 2981
SmoI	CTYRAG	4 cut(s)	112, 772, 2477, 2981
SphI	GCATGC	2 cut(s)	617, 2644
SsiI	CCGC	7 cut(s)	13, 382, 749, 1070, 1906, 2542, 2696
SspMI	CTAG	3 cut(s)	978, 1452, 3158
SstI	GAGCTC	1 cut(s)	3074
StyD4I	CCNGG	3 cut(s)	474, 1673, 3230
TaiI	ACGT	1 cut(s)	2637
TaqII	GACCGA	1 cut(s)	20
TatI	WGTACW	6 cut(s)	195, 510, 926, 2065, 2755, 3171
TauI	GCSGC	4 cut(s)	385, 1073, 1909, 2544
TfiI	GAWTC	4 cut(s)	969, 1879, 2294, 3525
TseFI	GTSAC	9 cut(s)	625, 830, 1641, 2047, 2119, 2618, 3163, 3376, 3455
TseI	GCWGC	5 cut(s)	1655, 1670, 2538, 3406, 3541
Tsp45I	GTSAC	9 cut(s)	625, 830, 1641, 2047, 2119, 2618, 3163, 3376, 3455
TspGWI	ACGGA	7 cut(s)	282, 570, 603, 2091, 2290, 2720, 2979
Vha464I	CTTAAG	1 cut(s)	772
VpaK11BI	GGWCC	2 cut(s)	496, 688
VspI	ATTAAT	1 cut(s)	2400
XagI	CCTNNNNNAGG	1 cut(s)	1859
XapI	RAATTY	6 cut(s)	208, 558, 1559, 2003, 2468, 2494
XceI	RCATGY	4 cut(s)	617, 1302, 2644, 2899
XhoI	CTCGAG	1 cut(s)	112
XmaJI	CCTAGG	1 cut(s)	3157
XspI	CTAG	3 cut(s)	978, 1452, 3158
ZrmI	AGTACT	2 cut(s)	512, 928
Zsp2I	ATGCAT	1 cut(s)	619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.