Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0011023

Physical Location & Seq

Genomic Coordinates Forward (+)

2960053 .. 2966575

Gene Structure 6523 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00080090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3426 bp

ATGCCGAGAGGGCGAAGGAGGACGATACATTTCAGTAAATTGTATTCGTTTTCGTGTATTCGGTCTTCTTTCGAAGATGTTCATGCTAAAATTGGGGATAGGGGGTACTCTAGGGTTGTGTACTGTAATGATCCTGATAATTCTGAGGCGCTGCAGTTGAGGTACAGGGGGAACTATGTTTCGACTACCAAGTATACGGCGGCTAATTTCATTCCAAAGTCTTTGTTTGAGCAGTTTAGGAGGGTGGCAAATATATACTTTCTTGTTGTGGCTTTTGTTTCGTTCAGTCCGTTGGCGCCTTTTAAGGCTGTTAGTGTTCTTATACCGCTATTGGTGGTGATTGGAGCTACTATGGTTAAGGAAGCTATAGAAGATTGGAGGCGAAGAAAACAGGATGTGGAGGCAAACAATCGAAAGGTGAAAGTGTATGGTAGGAATCATACATTCATTGAAACCAGATGGAAGAAACTCAGAGTTGGTGATCTTGTGAAGGTGCACAAGGATGAGTACTTTCCTGCTGATCTGCTTTTGCTTTCATCAAGCTATGACGATGGCATTTGCTATGTGGAAACTATGAACCTTGATGGAGAAACAAATTTAAAATTGAAGCATGCACTGGAGGCGACATCCCCTCTTCAAGATGAAAATTCCTTGGAAAGATTTGGGGCAGTGATCAAGTGTGAGGACCCAAATGAAAATCTATATTCATTTGTTGGGACTTTGTTCTATAACGGCAGAGAATACCCACTTTCCTTGCAACAGATGCTCCTAAGAGACTCTAAGCTGAAAAATACAGAATATGTCTATGGAGTTGTTGTTTTCACTGGGCATGACACAAAAGTCATGGAAAATGCTACAGATCCTCCTTCAAAGAGAAGTAAAATTGAGAGGAAAATGGATAAGATAATCTACATCCTTTTCAGTACTCTATTTGTGATAGCTTTCGTTGGATCCGTCTTTTTTGGAATTGATACCAAGAAAGATTATAGCGGTGGAAAGATGAGAAGGTGGTATCTTCGTCCAGATGATTCCGCTGTGTATTATGACCCCAAAAGGCCAGAAATTGCAGCTATTTTGCATTTTCTGACAGCTCTTATGTTGTATGGATATCTGATACCAATATCTTTGTATGTTTCAATTGAGATGGTGAAGGTATTACAGAGTATTTTCATTAACCGAGATCAAGATATGTACTATGAGGAAACAGATAGGCCAGCTCATGCACGCACCTCTAATCTGAATGAGGAACTTGGCCAGATTGATATGATACTGTCTGACAAAACTGGTACATTGACATGCAACTCCATGGAGTTTATCAAATGTTCGATAGCAGGCACTGCTTATGGCCATGGTATGACAGAAGTGGAAATGGCACTGGCAAACAGAAGGAACGGAGTGGCTGAAACTGGTCATTTATCATCTGATGTGGTAGAGCATAGTACTGGTGATGCTGATAGCTCTCGAAAGTCAATTAAGGGTTTCAACTTCAGGGATGGGCGCATCATGAATGGGCACTGGGTTAATGAACCTCATTCAGATACCATCCAGAAATTCCTTCGGGTTTTAGCAATCTGTCATACAGCCATACCTGTTGTGGATAAAGAATCAGGAGAAATATCCTATGAAGCTGAGTCACCAGATGAAGCAGCTTTTGTCATAGCTGCCAGGGAGCTCGGCTTTGAAGAATATGAGCTTCTTCAAGTCTTAGAGTTCAGTAGTTCTCGCAAAAGAATGTCAGTAATTGTTAAGAGTCCGGAAAACAAATTATTGCTTCTTTGCAAAGGTGCAGACAGTGCAATTTTTGAAAGGCTGGCAAAAGATGGGCGGCAGTTTGAGGATCAAACTAAGGAGCACATTCACAAATATGCTGAAGCAGGTTTACGAACCTTGGTGGTTGCACACCGTGAGCTTGGTCTAGAAGAATATAAAGCATGGGAACAAAAGTTTTTAAAGGCCAAAGCTTCTCTCACTGAAGGTCGAGATGCATTAATGGATGAGCTTGCTGATAAGATTGAAAGGGAATTAATACTACTTGGTGTGACAGCTGTTGAGGACAAACTGCAAAAGGGGGTTCCTGAGTATGCTTGCAGTTTACTTAGACAAGATATGAAACGGATTGTCATCAGTCTTGATTCGCCAGATATAATTGCCTTGGAAAAACAAGGGAAGAAAGACGCAATTCAGAAGGCCTGTCTTGCAAGCATAAGGAAGCAAATTGAAGAAGGTTTTTCGCAAATTAATGAAGCTAAAAAAGGTTCCAGCTCCACTAAAGCATTTGGCTTAATAATTGATGGAAAGTCCTTGGAATATTCACTTAAGAAGGACCTGGAAAAGTCATTTTTTGAGCTTGCAATTAATTGTGCTTCAGTTATATGCTGCCGCTCTACACCCAAACAGAAAGCTCTTGTTACGAGACTGGTAAAAAATGAGACTGGTAAAACAACACTTGCTGTTGGTGATGGGGCGAATGATGTTGGCATGCTTCAAGAGGCAGATATTGGAGTTGGCATTAGTGGTGTTGAAGGGATGCAGGCAGTGATGGCAAGTGATTTTGCAATAGCTCAATTTCGTTTTCTGGAGCGTCTCTTGCTTGTACACGGGCATTGGTGTTACAGGCGTATATCAATGATGATATGCTACTTCTTCTACAAGAACATAACATTCGGGTTTACTCTGTTTTGGTTTGAGGCCCATAATTCTTTCTCTGGTCTACCTGCTTATAATGATTGGTACATGTCATTCTACAATGTCTTCTTCACATCACTTCCTGTAATTGCTCTTGGTGTCTTCGATCAGGATGTTTCTGCACGGCTATGTCTGAAGTACCCATCTTTGTATCTAGAGGGAGTGGAGAACATCCTCTTCAGCTGGACCCGCATACTCGGTTGGATGTTTAATGGATTGTTGAGCTCCATAATAATCTTCTTCTTCACCACCAATTCTATGATTCCACAGCCGTTTCAGAAAGATGGTCAAGTAGCTGACTATGAAGTCCTAGGTGTCACAATGTACACTTGCGTAGTGTGGGCTGTAAACTGCCAAATGGCAATCTCCATCAACTACTTCACTTGGATCCAGCACTTGTTCATCTGGGGCAGTGTCGCCTTCTGGTACATATTTTTAGTCATATATGGTTCTGTCTCACCTAGTATATCCACAACAGCATACAAAGTGCTCGTGGAAGAGTGTGCTCCCAGTCCTCTTTATTGGCTGGTCACCCTTCTTGTTGTTGTCTGCACTCTGCTGCCATATTTTTCATACCGTGCTTTCCAGACACGGTTTAAACCAATGCGTCACGACGTAATACAGCAGAAACGATTACTAAGTTGTAACAGTGATCAGATTGATGCTTCTGGTGAATTGCCTGTAAGAGTCAGGGGCAAGATACGGCATCTGAGGCAGAGATTAAGAGAAAGAGAGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1141

Amino Acids

130.06

Weight (kDa)

7.89

Isoelectric Point (pI)

41.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	40 - 105	4.5e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	515 - 689	1.5e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	846 - 1096	7.7e-93	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G27870 AT3G27870 AT3G27870
fragaria_vesca	FvH4_6g50810
malus_domestica	MD09G1032100.v1.1 MD17G1033800.v1.1
prunus_persica	Prupe.3G285900_v2.0.a1 Prupe.3G285900_v2.0.a1
pyrus_communis	pycom111g02540
rosa_chinensis	RchiOBHm_Chr2g0172201
rosa_laevigata	RLG00000022092
rosa_multiflora	Rmu_ssc0000252.1_g000050
rosa_roxburghii	Rroxscaffold_2G00080090
rosa_rugosa	Rorug02G0557000 Rorug02G0557100 Rorug02G0557200
rosa_samantha	Rh2AG632600 Rh2BG646200 Rh2DG660800
rosa_wichuraiana	Rw2G052370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2721
AasI	GACNNNNNNGTC	2 cut(s)	839, 2990
Acc36I	ACCTGC	2 cut(s)	1865, 2722
AccB1I	GGYRCC	1 cut(s)	295
AccBSI	CCGCTC	1 cut(s)	2382
AccI	GTMKAC	2 cut(s)	194, 2710
AccIII	TCCGGA	1 cut(s)	1753
AciI	CCGC	7 cut(s)	200, 326, 990, 1032, 1825, 2380, 2875
AclWI	GGATC	7 cut(s)	125, 854, 945, 958, 1845, 3067, 3080
AcoI	YGGCCR	2 cut(s)	1252, 1345
AcsI	RAATTY	3 cut(s)	595, 646, 1550
AcuI	CTGAAG	6 cut(s)	1471, 1890, 1992, 2349, 2840, 2848
AcyI	GRCGYC	1 cut(s)	296
AdeI	CACNNNGTG	1 cut(s)	1904
AflII	CTTAAG	1 cut(s)	2315
AflIII	ACRYGT	1 cut(s)	2733
AjnI	CCWGG	2 cut(s)	1664, 2325
Alw21I	GWGCWC	6 cut(s)	498, 1674, 1854, 2912, 3177, 3193
Alw26I	GTCTC	5 cut(s)	768, 2407, 2423, 2588, 3145
Alw44I	GTGCAC	1 cut(s)	494
AlwI	GGATC	7 cut(s)	125, 854, 945, 958, 1845, 3067, 3080
AlwNI	CAGNNNCTG	1 cut(s)	1337
Aor13HI	TCCGGA	1 cut(s)	1753
AoxI	GGCC	7 cut(s)	1055, 1211, 1252, 1345, 1953, 2187, 2688
ApaLI	GTGCAC	1 cut(s)	494
ApeKI	GCWGC	6 cut(s)	151, 1067, 1646, 1661, 2376, 3244
ApoI	RAATTY	3 cut(s)	595, 646, 1550
AseI	ATTAAT	4 cut(s)	1988, 2024, 2238, 2355
Asp700I	GAANNNNTTC	1 cut(s)	78
AspA2I	CCTAGG	1 cut(s)	2995
AspLEI	GCGC	3 cut(s)	151, 298, 1500
AspS9I	GGNCC	4 cut(s)	685, 2323, 2689, 2871
AsuII	TTCGAA	1 cut(s)	72
AvaII	GGWCC	3 cut(s)	685, 2323, 2871
AvrII	CCTAGG	1 cut(s)	2995
BaeGI	GKGCMC	2 cut(s)	498, 1515
BalI	TGGCCA	2 cut(s)	1254, 1347
BamHI	GGATCC	2 cut(s)	950, 3072
BanI	GGYRCC	1 cut(s)	295
BanII	GRGCYC	2 cut(s)	1674, 2912
BarI	GAAGNNNNNNTAC	4 cut(s)	49, 81, 1863, 1895
BauI	CACGAG	1 cut(s)	3176
BbsI	GAAGAC	3 cut(s)	57, 2743, 2779
Bbv12I	GWGCWC	6 cut(s)	498, 1674, 1854, 2912, 3177, 3193
BbvI	GCAGC	6 cut(s)	138, 1079, 1648, 1658, 2363, 3231
BceAI	ACGGC	5 cut(s)	213, 748, 2825, 2941, 3404
BciT130I	CCWGG	2 cut(s)	1666, 2327
BclI	TGATCA	2 cut(s)	672, 3337
BcoDI	GTCTC	5 cut(s)	768, 2407, 2423, 2588, 3145
BfaI	CTAG	5 cut(s)	111, 1916, 2840, 2996, 3147
BfmI	CTRYAG	3 cut(s)	152, 366, 855
BfoI	RGCGCY	2 cut(s)	152, 299
BfrI	CTTAAG	1 cut(s)	2315
BfuAI	ACCTGC	2 cut(s)	1865, 2722
BglI	GCCNNNNNGGC	1 cut(s)	10
BisI	GCNGC	9 cut(s)	152, 201, 1068, 1647, 1662, 1826, 2377, 2380, 3245
BlnI	CCTAGG	1 cut(s)	2995
BlsI	GCNGC	9 cut(s)	153, 202, 1069, 1648, 1663, 1827, 2378, 2381, 3246
BmcAI	AGTACT	3 cut(s)	509, 925, 1441
Bme1390I	CCNGG	2 cut(s)	1666, 2327
Bme18I	GGWCC	3 cut(s)	685, 2323, 2871
BmgT120I	GGNCC	4 cut(s)	685, 2323, 2689, 2871
BmiI	GGNNCC	7 cut(s)	297, 687, 952, 2073, 2257, 2873, 3074
BmrFI	CCNGG	2 cut(s)	1666, 2327
BmrI	ACTGGG	3 cut(s)	834, 1525, 3189
BmsI	GCATC	7 cut(s)	753, 1438, 1509, 1972, 2517, 3337, 3400
BmuI	ACTGGG	3 cut(s)	834, 1525, 3189
BpiI	GAAGAC	3 cut(s)	57, 2743, 2779
BpmI	CTGGAG	2 cut(s)	638, 2597
Bpu14I	TTCGAA	1 cut(s)	72
BsaBI	GATNNNNATC	1 cut(s)	2120
BsaHI	GRCGYC	1 cut(s)	296
BsaJI	CCNNGG	8 cut(s)	651, 1305, 1348, 1665, 1887, 2151, 2301, 2995
BsaWI	WCCGGW	1 cut(s)	1753
BsaXI	ACNNNNNCTCC	6 cut(s)	750, 780, 801, 831, 847, 877
Bse8I	GATNNNNATC	1 cut(s)	2120
BseAI	TCCGGA	1 cut(s)	1753
BseBI	CCWGG	2 cut(s)	1666, 2327
BseDI	CCNNGG	8 cut(s)	651, 1305, 1348, 1665, 1887, 2151, 2301, 2995
BseJI	GATNNNNATC	1 cut(s)	2120
BseMII	CTCAG	5 cut(s)	135, 484, 1620, 2067, 3386
BseSI	GKGCMC	2 cut(s)	498, 1515
BseXI	GCAGC	6 cut(s)	138, 1079, 1648, 1658, 2363, 3231
BsgI	GTGCAG	3 cut(s)	1806, 2790, 3220
BshFI	GGCC	7 cut(s)	1057, 1213, 1254, 1347, 1955, 2189, 2690
BshNI	GGYRCC	1 cut(s)	295
BsiHKAI	GWGCWC	6 cut(s)	498, 1674, 1854, 2912, 3177, 3193
BsiSI	CCGG	1 cut(s)	1754
BslFI	GGGAC	1 cut(s)	730
BsmAI	GTCTC	5 cut(s)	768, 2407, 2423, 2588, 3145
BsmBI	CGTCTC	1 cut(s)	2588
BsmFI	GGGAC	1 cut(s)	730
BsnI	GGCC	7 cut(s)	1057, 1213, 1254, 1347, 1955, 2189, 2690
Bsp119I	TTCGAA	1 cut(s)	72
Bsp1286I	GDGCHC	7 cut(s)	498, 1515, 1674, 1854, 2912, 3177, 3193
Bsp13I	TCCGGA	1 cut(s)	1753
Bsp1407I	TGTACA	2 cut(s)	2593, 3009
Bsp19I	CCATGG	2 cut(s)	1305, 1348
BspACI	CCGC	7 cut(s)	200, 326, 990, 1032, 1825, 2380, 2875
BspANI	GGCC	7 cut(s)	1057, 1213, 1254, 1347, 1955, 2189, 2690
BspCNI	CTCAG	5 cut(s)	136, 483, 1621, 2068, 3387
BspEI	TCCGGA	1 cut(s)	1753
BspHI	TCATGA	2 cut(s)	1503, 3422
BspLI	GGNNCC	7 cut(s)	297, 687, 952, 2073, 2257, 2873, 3074
BspMAI	CTGCAG	1 cut(s)	156
BspMI	ACCTGC	2 cut(s)	1865, 2722
BspPI	GGATC	7 cut(s)	125, 854, 945, 958, 1845, 3067, 3080
BspT104I	TTCGAA	1 cut(s)	72
BspT107I	GGYRCC	1 cut(s)	295
BspTI	CTTAAG	1 cut(s)	2315
BsrBI	CCGCTC	1 cut(s)	2382
BsrGI	TGTACA	2 cut(s)	2593, 3009
BssECI	CCNNGG	8 cut(s)	651, 1305, 1348, 1665, 1887, 2151, 2301, 2995
BssNAI	GTATAC	1 cut(s)	195
BssNI	GRCGYC	1 cut(s)	296
BssSI	CACGAG	1 cut(s)	3176
BssT1I	CCWWGG	7 cut(s)	651, 1305, 1348, 1887, 2151, 2301, 2995
Bst1107I	GTATAC	1 cut(s)	195
Bst2BI	CACGAG	1 cut(s)	3176
Bst2UI	CCWGG	2 cut(s)	1666, 2327
Bst4CI	ACNGT	7 cut(s)	125, 1272, 1793, 1904, 3263, 3279, 3335
Bst6I	CTCTTC	3 cut(s)	639, 2867, 3177
BstACI	GRCGYC	1 cut(s)	296
BstAFI	CTTAAG	1 cut(s)	2315
BstAPI	GCANNNNNTGC	3 cut(s)	763, 1337, 1793
BstAUI	TGTACA	2 cut(s)	2593, 3009
BstBI	TTCGAA	1 cut(s)	72
BstDSI	CCRYGG	2 cut(s)	1305, 1348
BstEII	GGTNACC	1 cut(s)	3214
BstH2I	RGCGCY	2 cut(s)	152, 299
BstHHI	GCGC	3 cut(s)	151, 298, 1500
BstMAI	GTCTC	5 cut(s)	768, 2407, 2423, 2588, 3145
BstNI	CCWGG	2 cut(s)	1666, 2327
BstNSI	RCATGY	4 cut(s)	614, 1299, 2482, 2737
BstPI	GGTNACC	1 cut(s)	3214
BstSCI	CCNGG	2 cut(s)	1664, 2325
BstSFI	CTRYAG	3 cut(s)	152, 366, 855
BstSLI	GKGCMC	2 cut(s)	498, 1515
BstV1I	GCAGC	6 cut(s)	138, 1079, 1648, 1658, 2363, 3231
BstV2I	GAAGAC	3 cut(s)	57, 2743, 2779
BstX2I	RGATCY	3 cut(s)	859, 950, 3072
BstYI	RGATCY	3 cut(s)	859, 950, 3072
BstZ17I	GTATAC	1 cut(s)	195
BsuRI	GGCC	7 cut(s)	1057, 1213, 1254, 1347, 1955, 2189, 2690
BtgI	CCRYGG	2 cut(s)	1305, 1348
BtsI	GCAGTG	4 cut(s)	675, 1335, 2541, 3103
BveI	ACCTGC	2 cut(s)	1865, 2722
CaiI	CAGNNNCTG	1 cut(s)	1337
CciI	TCATGA	2 cut(s)	1503, 3422
CfoI	GCGC	3 cut(s)	151, 298, 1500
Cfr13I	GGNCC	4 cut(s)	685, 2323, 2689, 2871
CseI	GACGC	3 cut(s)	2183, 2570, 3281
CspCI	CAANNNNNGTGG	2 cut(s)	735, 770
DinI	GGCGCC	1 cut(s)	297
DraI	TTTAAA	3 cut(s)	600, 1950, 3283
DraIII	CACNNNGTG	1 cut(s)	1904
DrdI	GACNNNNNNGTC	2 cut(s)	839, 2990
DseDI	GACNNNNNNGTC	2 cut(s)	839, 2990
EaeI	YGGCCR	2 cut(s)	1252, 1345
Eam1104I	CTCTTC	3 cut(s)	639, 2867, 3177
EarI	CTCTTC	3 cut(s)	639, 2867, 3177
Ecl136II	GAGCTC	2 cut(s)	1672, 2910
Eco130I	CCWWGG	7 cut(s)	651, 1305, 1348, 1887, 2151, 2301, 2995
Eco147I	AGGCCT	1 cut(s)	2189
Eco24I	GRGCYC	2 cut(s)	1674, 2912
Eco32I	GATATC	1 cut(s)	1109
Eco47I	GGWCC	3 cut(s)	685, 2323, 2871
Eco53kI	GAGCTC	2 cut(s)	1672, 2910
Eco57I	CTGAAG	6 cut(s)	1471, 1890, 1992, 2349, 2840, 2848
Eco91I	GGTNACC	1 cut(s)	3214
EcoICRI	GAGCTC	2 cut(s)	1672, 2910
EcoO109I	RGGNCCY	2 cut(s)	685, 2323
EcoO65I	GGTNACC	1 cut(s)	3214
EcoRII	CCWGG	2 cut(s)	1664, 2325
EcoRV	GATATC	1 cut(s)	1109
EcoT14I	CCWWGG	7 cut(s)	651, 1305, 1348, 1887, 2151, 2301, 2995
EcoT22I	ATGCAT	1 cut(s)	1987
EcoT38I	GRGCYC	2 cut(s)	1674, 2912
EgeI	GGCGCC	1 cut(s)	297
EheI	GGCGCC	1 cut(s)	297
ErhI	CCWWGG	7 cut(s)	651, 1305, 1348, 1887, 2151, 2301, 2995
Esp3I	CGTCTC	1 cut(s)	2588
FaqI	GGGAC	1 cut(s)	730
FauI	CCCGC	1 cut(s)	2882
FbaI	TGATCA	2 cut(s)	672, 3337
FblI	GTMKAC	2 cut(s)	194, 2710
Fnu4HI	GCNGC	9 cut(s)	152, 201, 1068, 1647, 1662, 1826, 2377, 2380, 3245
FriOI	GRGCYC	2 cut(s)	1674, 2912
Fsp4HI	GCNGC	9 cut(s)	152, 201, 1068, 1647, 1662, 1826, 2377, 2380, 3245
FspBI	CTAG	5 cut(s)	111, 1916, 2840, 2996, 3147
GlaI	GCGC	3 cut(s)	150, 297, 1499
GluI	GCNGC	9 cut(s)	152, 201, 1068, 1647, 1662, 1826, 2377, 2380, 3245
GsuI	CTGGAG	2 cut(s)	638, 2597
HaeII	RGCGCY	2 cut(s)	152, 299
HaeIII	GGCC	7 cut(s)	1057, 1213, 1254, 1347, 1955, 2189, 2690
HapII	CCGG	1 cut(s)	1754
HgaI	GACGC	3 cut(s)	2183, 2570, 3281
HhaI	GCGC	3 cut(s)	151, 298, 1500
Hin1I	GRCGYC	1 cut(s)	296
Hin6I	GCGC	3 cut(s)	149, 296, 1498
HinP1I	GCGC	3 cut(s)	149, 296, 1498
HindIII	AAGCTT	1 cut(s)	1959
HpaII	CCGG	1 cut(s)	1754
Hpy99I	CGWCG	1 cut(s)	3302
HpyCH4III	ACNGT	7 cut(s)	125, 1272, 1793, 1904, 3263, 3279, 3335
HpyCH4IV	ACGT	1 cut(s)	3300
HpySE526I	ACGT	1 cut(s)	3300
Hsp92I	GRCGYC	1 cut(s)	296
HspAI	GCGC	3 cut(s)	149, 296, 1498
KasI	GGCGCC	1 cut(s)	295
Kpn2I	TCCGGA	1 cut(s)	1753
Ksp22I	TGATCA	2 cut(s)	672, 3337
LmnI	GCTCC	8 cut(s)	344, 771, 1669, 1849, 2267, 2578, 2915, 3196
Lsp1109I	GCAGC	6 cut(s)	138, 1079, 1648, 1658, 2363, 3231
LweI	GCATC	7 cut(s)	753, 1438, 1509, 1972, 2517, 3337, 3400
MaeI	CTAG	5 cut(s)	111, 1916, 2840, 2996, 3147
MaeII	ACGT	1 cut(s)	3300
MaeIII	GTNAC	8 cut(s)	1632, 2038, 2407, 2609, 3001, 3214, 3293, 3329
MbiI	CCGCTC	1 cut(s)	2382
MfeI	CAATTG	1 cut(s)	1137
MflI	RGATCY	3 cut(s)	859, 950, 3072
MhlI	GDGCHC	7 cut(s)	498, 1515, 1674, 1854, 2912, 3177, 3193
MlsI	TGGCCA	2 cut(s)	1254, 1347
MluNI	TGGCCA	2 cut(s)	1254, 1347
Mly113I	GGCGCC	1 cut(s)	296
MlyI	GAGTC	4 cut(s)	770, 1640, 1759, 3381
MmeI	TCCRAC	2 cut(s)	928, 2867
Mox20I	TGGCCA	2 cut(s)	1254, 1347
Mph1103I	ATGCAT	1 cut(s)	1987
MroI	TCCGGA	1 cut(s)	1753
MroXI	GAANNNNTTC	1 cut(s)	78
MscI	TGGCCA	2 cut(s)	1254, 1347
MslI	CAYNNNNRTG	4 cut(s)	501, 1294, 1863, 2812
Msp20I	TGGCCA	2 cut(s)	1254, 1347
MspA1I	CMGCKG	3 cut(s)	1034, 2045, 2868
MspCI	CTTAAG	1 cut(s)	2315
MspI	CCGG	1 cut(s)	1754
MspR9I	CCNGG	2 cut(s)	1666, 2327
MssI	GTTTAAAC	1 cut(s)	3283
MunI	CAATTG	1 cut(s)	1137
MvaI	CCWGG	2 cut(s)	1666, 2327
NarI	GGCGCC	1 cut(s)	296
NcoI	CCATGG	2 cut(s)	1305, 1348
NlaIV	GGNNCC	7 cut(s)	297, 687, 952, 2073, 2257, 2873, 3074
NmeAIII	GCCGAG	2 cut(s)	30, 1653
NmuCI	GTSAC	5 cut(s)	1632, 2038, 3001, 3214, 3293
NsiI	ATGCAT	1 cut(s)	1987
NspI	RCATGY	4 cut(s)	614, 1299, 2482, 2737
NspV	TTCGAA	1 cut(s)	72
PaeI	GCATGC	2 cut(s)	614, 2482
PagI	TCATGA	2 cut(s)	1503, 3422
PceI	AGGCCT	1 cut(s)	2189
PciI	ACATGT	1 cut(s)	2733
PcsI	WCGNNNNNNNCGW	2 cut(s)	287, 951
PdmI	GAANNNNTTC	1 cut(s)	78
PfeI	GAWTC	5 cut(s)	436, 1028, 1604, 2132, 2947
PkrI	GCNGC	9 cut(s)	153, 202, 1069, 1648, 1663, 1827, 2378, 2381, 3246
PleI	GAGTC	4 cut(s)	770, 1639, 1758, 3380
PluTI	GGCGCC	1 cut(s)	299
PmeI	GTTTAAAC	1 cut(s)	3283
PpsI	GAGTC	4 cut(s)	770, 1639, 1758, 3380
PpuMI	RGGWCCY	2 cut(s)	685, 2323
PscI	ACATGT	1 cut(s)	2733
PshBI	ATTAAT	4 cut(s)	1988, 2024, 2238, 2355
PsiI	TTATAA	1 cut(s)	2721
Psp124BI	GAGCTC	2 cut(s)	1674, 2912
Psp5II	RGGWCCY	2 cut(s)	685, 2323
Psp6I	CCWGG	2 cut(s)	1664, 2325
PspEI	GGTNACC	1 cut(s)	3214
PspGI	CCWGG	2 cut(s)	1664, 2325
PspN4I	GGNNCC	7 cut(s)	297, 687, 952, 2073, 2257, 2873, 3074
PspPI	GGNCC	4 cut(s)	685, 2323, 2689, 2871
PspPPI	RGGWCCY	2 cut(s)	685, 2323
PstI	CTGCAG	1 cut(s)	156
PstNI	CAGNNNCTG	1 cut(s)	1337
PsuI	RGATCY	3 cut(s)	859, 950, 3072
PvuII	CAGCTG	2 cut(s)	2045, 2868
RseI	CAYNNNNRTG	4 cut(s)	501, 1294, 1863, 2812
SacI	GAGCTC	2 cut(s)	1674, 2912
SatI	GCNGC	9 cut(s)	152, 201, 1068, 1647, 1662, 1826, 2377, 2380, 3245
Sau96I	GGNCC	4 cut(s)	685, 2323, 2689, 2871
ScaI	AGTACT	3 cut(s)	509, 925, 1441
SchI	GAGTC	4 cut(s)	770, 1640, 1759, 3381
ScrFI	CCNGG	2 cut(s)	1666, 2327
SduI	GDGCHC	7 cut(s)	498, 1515, 1674, 1854, 2912, 3177, 3193
SfaNI	GCATC	7 cut(s)	753, 1438, 1509, 1972, 2517, 3337, 3400
SfcI	CTRYAG	3 cut(s)	152, 366, 855
SfoI	GGCGCC	1 cut(s)	297
SfuI	TTCGAA	1 cut(s)	72
SinI	GGWCC	3 cut(s)	685, 2323, 2871
SmiMI	CAYNNNNRTG	4 cut(s)	501, 1294, 1863, 2812
SmlI	CTYRAG	1 cut(s)	2315
SmoI	CTYRAG	1 cut(s)	2315
SphI	GCATGC	2 cut(s)	614, 2482
SseBI	AGGCCT	1 cut(s)	2189
SsiI	CCGC	7 cut(s)	200, 326, 990, 1032, 1825, 2380, 2875
SspDI	GGCGCC	1 cut(s)	295
SspI	AATATT	1 cut(s)	2309
SspMI	CTAG	5 cut(s)	111, 1916, 2840, 2996, 3147
SstI	GAGCTC	2 cut(s)	1674, 2912
StuI	AGGCCT	1 cut(s)	2189
StyD4I	CCNGG	2 cut(s)	1664, 2325
StyI	CCWWGG	7 cut(s)	651, 1305, 1348, 1887, 2151, 2301, 2995
TaaI	ACNGT	7 cut(s)	125, 1272, 1793, 1904, 3263, 3279, 3335
TaiI	ACGT	1 cut(s)	3303
TaqI	TCGA	7 cut(s)	72, 182, 412, 1325, 1462, 1978, 2790
TaqII	GACCGA	1 cut(s)	51
TatI	WGTACW	7 cut(s)	120, 507, 923, 1191, 1439, 2593, 3009
TauI	GCSGC	3 cut(s)	203, 1828, 2382
TfiI	GAWTC	5 cut(s)	436, 1028, 1604, 2132, 2947
TseFI	GTSAC	5 cut(s)	1632, 2038, 3001, 3214, 3293
TseI	GCWGC	6 cut(s)	151, 1067, 1646, 1661, 2376, 3244
Tsp45I	GTSAC	5 cut(s)	1632, 2038, 3001, 3214, 3293
TspGWI	ACGGA	4 cut(s)	279, 943, 1407, 2128
Vha464I	CTTAAG	1 cut(s)	2315
VneI	GTGCAC	1 cut(s)	494
VpaK11BI	GGWCC	3 cut(s)	685, 2323, 2871
VspI	ATTAAT	4 cut(s)	1988, 2024, 2238, 2355
XapI	RAATTY	3 cut(s)	595, 646, 1550
XbaI	TCTAGA	2 cut(s)	1915, 2839
XceI	RCATGY	4 cut(s)	614, 1299, 2482, 2737
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1591
XmaJI	CCTAGG	1 cut(s)	2995
XmiI	GTMKAC	2 cut(s)	194, 2710
XmnI	GAANNNNTTC	1 cut(s)	78
XspI	CTAG	5 cut(s)	111, 1916, 2840, 2996, 3147
ZrmI	AGTACT	3 cut(s)	509, 925, 1441
Zsp2I	ATGCAT	1 cut(s)	1987

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.