MD09G1040800.v1.1

Mannose-1-phosphate guanyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012792
Physical Location & Seq
Forward (+)
2627642 .. 2633412
5771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1040800.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGGGGAGAAGGTAGTCGCTGTAATCATGGTGGGCGGGCCCACCAAAGGGACTAGGTTTCGGCCTCTGTCATTCAACACTCCGAAACCGCTGTTCCCATTGGCTGGGCAGGCTATGGTGTACCACCCCATTGCAGCTTGTAAAAAGATACCCAATTTAGCACAAATCTATTTGGTTGGATTCTATGAAGAGAAAGAATTCGCACTGTACGTGTCTTCCATTTCCAATGAGCTCAGAGTGCCAGTGAGGTACTTGAGAGAGGACAAACCGCACGGCTCGGCCGGCGGACTGTATTACTACAGAGATATCATCATGGAAGACAGCCCGTCGCACATCTTTTTGCTGAATTGTGATGTTTGCTGCAGTTTTCCGCTTGCAGACATGCTTGAGGCTCATAAAGGATATAAGGGGATGGGTACAATGTTAGTAATTAAGGTTTCTGCTGAATCTGCAAACCAGTTTGGGGAGTTGGTTGCTGATCCTGCCACCAAAGAATTATTGCATTATACTGAGAAGCCAGAGACTTTTGTGAGTGATTTGATCAACTGTGGTGTATATGTATTTACTCCAGATATTTTTTCTGCCATTCAAGAAGTCTCCAGTCACCGAGAAGGCAGAGCTAATTTACGTCGTGTTTCCAGCTTTGAAGCCCTCCAGTCCGCAACAAGGACTCTTCCTACAGATTTTGTTAGATTGGATCAAGATATTTTGTCACCTCTTGCTGGAAAGAAGAAACTGTATACATATGAGACCATGGATTTTTGGGAACAGATCAAGACTCCTGGAATGTCTTTAAAATGTTCGGGTTTGTATCTTTCTCAATTCCAAATTAACTCCGAACACCTTTTAGCTAGTGGAGATGGTACCAAGAATGCTAGAATTGTTGGTAATGTTTATGTTCATCCATCGGCAAAAGTACATCCAACTTCTAAGATTGGTCCCGATGTGTCTCTTTCAGCAAATGTTCGTGTAGGGGCAGGAGTAAGGCTTATAAATTGCATTGTCTTGGATGATGTGGAAATCAAGGAAAATGCAGTCGTTATAAATGCTATTGTTGGATGGAAGTCGTCTATTGGAAAATGGTCGCGTGTCCAGGCTGACGGAGACTACAGCGCAAAGCTTGGAATTACCATCCTCGGAGAAGAGGTGACAGTTGAAGACGAAGTTGTGGTGATCAACAGCATCGTTCTCCCAAATAAGACTCTTAACGTTAGCGTACAGGAGGAAATCATTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 map00051 map00520 map01100 map01110
Pfam Domains
Protein Families
Hexapep NTP_transferase

Protein Analysis

413

Amino Acids

45.38

Weight (kDa)

6.03

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 7 - 204 5.5e-30 Nucleotidyl transferase
LbH_EIF2B PF25084 290 - 366 1.4e-06 EIF2B subunit epsilon LbH domain
GMPPB_C PF25087 294 - 411 7.3e-37 GMPPB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04650 AT2G04650
fragaria_vesca FvH4_6g49920
malus_domestica MD09G1040800.v1.1 MD17G1042000.v1.1
prunus_persica Prupe.3G276900_v2.0.a1 Prupe.3G276900_v2.0.a1
pyrus_communis pycom111g03180 pycom17g03750
rosa_chinensis RchiOBHm_Chr2g0170091
rosa_laevigata RLG00000021921
rosa_multiflora Rmu_sc0013453.1_g000005
rosa_roxburghii Rroxscaffold_2G00081590
rosa_rugosa Rorug02G0545700.1
rosa_samantha Rh2AG617800 Rh2BG629300 Rh2DG641100
rosa_wichuraiana Rw2G051150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 992, 1043
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 1 cut(s) 104
AccI GTMKAC 1 cut(s) 740
AccII CGCG 1 cut(s) 1087
AciI CCGC 6 cut(s) 36, 89, 269, 285, 371, 660
AclI AACGTT 1 cut(s) 1209
AclWI GGATC 2 cut(s) 473, 705
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 197
AfaI GTAC 7 cut(s) 122, 209, 251, 418, 865, 918, 1218
AfiI CCNNNNNNNGG 6 cut(s) 47, 48, 104, 463, 666, 722
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 4 cut(s) 76, 590, 647, 1157
AhdI GACNNNNNGTC 1 cut(s) 325
AjnI CCWGG 2 cut(s) 781, 1092
AluBI AGCT 6 cut(s) 137, 232, 620, 642, 851, 1120
AluI AGCT 6 cut(s) 137, 232, 620, 642, 851, 1120
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 5 cut(s) 515, 601, 743, 954, 1098
AlwI GGATC 2 cut(s) 473, 705
AoxI GGCC 3 cut(s) 38, 62, 279
ApaI GGGCCC 1 cut(s) 42
ApeKI GCWGC 2 cut(s) 134, 360
ApoI RAATTY 1 cut(s) 197
Asp700I GAANNNNTTC 2 cut(s) 197, 1230
Asp718I GGTACC 1 cut(s) 863
AspLEI GCGC 1 cut(s) 1115
AspS9I GGNCC 3 cut(s) 38, 39, 938
AsuHPI GGTGA 4 cut(s) 596, 705, 1159, 1183
AvaII GGWCC 1 cut(s) 938
BaeGI GKGCMC 1 cut(s) 42
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 2 cut(s) 42, 234
BbsI GAAGAC 3 cut(s) 207, 324, 1164
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 146, 347
BccI CCATC 5 cut(s) 406, 854, 913, 1053, 1139
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 2 cut(s) 783, 1094
BclI TGATCA 2 cut(s) 540, 1173
BcoDI GTCTC 5 cut(s) 515, 601, 743, 954, 1098
BfaI CTAG 3 cut(s) 54, 852, 876
BfmI CTRYAG 4 cut(s) 298, 361, 678, 1108
BisI GCNGC 2 cut(s) 135, 361
BlsI GCNGC 2 cut(s) 136, 362
Bme1390I CCNGG 2 cut(s) 783, 1094
Bme18I GGWCC 1 cut(s) 938
BmeRI GACNNNNNGTC 1 cut(s) 325
BmgT120I GGNCC 3 cut(s) 38, 39, 938
BmiI GGNNCC 3 cut(s) 40, 865, 940
BmrFI CCNGG 2 cut(s) 783, 1094
BmsI GCATC 1 cut(s) 1191
BpiI GAAGAC 3 cut(s) 207, 324, 1164
BpmI CTGGAG 3 cut(s) 552, 583, 638
BpuEI CTTGAG 2 cut(s) 274, 407
BsaAI YACGTR 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 743
BsaJI CCNNGG 2 cut(s) 753, 1135
BsaXI ACNNNNNCTCC 2 cut(s) 1131, 1161
Bsc4I CCNNNNNNNGG 6 cut(s) 47, 48, 104, 463, 666, 722
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 4 cut(s) 242, 457, 600, 655
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 2 cut(s) 783, 1094
BseDI CCNNGG 2 cut(s) 753, 1135
BseGI GGATG 6 cut(s) 417, 901, 919, 1015, 1064, 1131
BseLI CCNNNNNNNGG 6 cut(s) 47, 48, 104, 463, 666, 722
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 2 cut(s) 247, 501
BseNI ACTGG 4 cut(s) 242, 457, 600, 655
BseSI GKGCMC 1 cut(s) 42
BseX3I CGGCCG 1 cut(s) 279
BseXI GCAGC 2 cut(s) 146, 347
BseYI CCCAGC 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 1087
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 3 cut(s) 40, 64, 281
BshNI GGYRCC 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 1 cut(s) 282
BslFI GGGAC 2 cut(s) 64, 924
BslI CCNNNNNNNGG 6 cut(s) 47, 48, 104, 463, 666, 722
BsmAI GTCTC 5 cut(s) 515, 601, 743, 954, 1098
BsmFI GGGAC 2 cut(s) 64, 924
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 3 cut(s) 40, 64, 281
Bso31I GGTCTC 1 cut(s) 743
Bsp120I GGGCCC 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 42, 234
Bsp143I GATC 5 cut(s) 478, 540, 697, 771, 1173
Bsp19I CCATGG 1 cut(s) 753
BspACI CCGC 6 cut(s) 36, 89, 269, 285, 371, 660
BspANI GGCC 3 cut(s) 40, 64, 281
BspCNI CTCAG 2 cut(s) 246, 502
BspFNI CGCG 1 cut(s) 1087
BspLI GGNNCC 3 cut(s) 40, 865, 940
BspMAI CTGCAG 1 cut(s) 365
BspPI GGATC 2 cut(s) 473, 705
BspT107I GGYRCC 1 cut(s) 863
BspTNI GGTCTC 1 cut(s) 743
BsrDI GCAATG 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 4 cut(s) 242, 457, 600, 655
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 2 cut(s) 753, 1135
BssMI GATC 5 cut(s) 478, 540, 697, 771, 1173
BssNAI GTATAC 1 cut(s) 741
BssT1I CCWWGG 1 cut(s) 753
Bst1107I GTATAC 1 cut(s) 741
Bst2UI CCWGG 2 cut(s) 783, 1094
Bst4CI ACNGT 5 cut(s) 207, 291, 548, 738, 1153
Bst6I CTCTTC 3 cut(s) 183, 678, 1137
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 4 cut(s) 38, 111, 283, 375
BstDEI CTNAG 3 cut(s) 233, 510, 930
BstDSI CCRYGG 1 cut(s) 753
BstF5I GGATG 6 cut(s) 417, 901, 919, 1015, 1064, 1131
BstFNI CGCG 1 cut(s) 1087
BstHHI GCGC 1 cut(s) 1115
BstKTI GATC 5 cut(s) 481, 543, 700, 774, 1176
BstMAI GTCTC 5 cut(s) 515, 601, 743, 954, 1098
BstMBI GATC 5 cut(s) 478, 540, 697, 771, 1173
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 5 cut(s) 110, 238, 282, 449, 482
BstNI CCWGG 2 cut(s) 783, 1094
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 781, 1092
BstSFI CTRYAG 4 cut(s) 298, 361, 678, 1108
BstSLI GKGCMC 1 cut(s) 42
BstUI CGCG 1 cut(s) 1087
BstV1I GCAGC 2 cut(s) 146, 347
BstV2I GAAGAC 3 cut(s) 207, 324, 1164
BstZ17I GTATAC 1 cut(s) 741
BstZI CGGCCG 1 cut(s) 279
BsuRI GGCC 3 cut(s) 40, 64, 281
BtgI CCRYGG 1 cut(s) 753
BtsCI GGATG 6 cut(s) 417, 901, 919, 1015, 1064, 1131
BtsIMutI CAGTG 2 cut(s) 203, 249
Cac8I GCNNGC 4 cut(s) 38, 111, 283, 375
CfoI GCGC 1 cut(s) 1115
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 3 cut(s) 38, 39, 938
Csp6I GTAC 7 cut(s) 121, 208, 250, 417, 864, 917, 1217
CviAII CATG 4 cut(s) 28, 313, 382, 754
CviQI GTAC 7 cut(s) 121, 208, 250, 417, 864, 917, 1217
DdeI CTNAG 3 cut(s) 233, 510, 930
DpnI GATC 5 cut(s) 480, 542, 699, 773, 1175
DpnII GATC 5 cut(s) 478, 540, 697, 771, 1173
DraI TTTAAA 1 cut(s) 795
DriI GACNNNNNGTC 1 cut(s) 325
EaeI YGGCCR 1 cut(s) 279
EagI CGGCCG 1 cut(s) 279
Eam1104I CTCTTC 3 cut(s) 183, 678, 1137
Eam1105I GACNNNNNGTC 1 cut(s) 325
EarI CTCTTC 3 cut(s) 183, 678, 1137
EciI GGCGGA 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 232
EclXI CGGCCG 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 753
Eco24I GRGCYC 2 cut(s) 42, 234
Eco31I GGTCTC 1 cut(s) 743
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 938
Eco52I CGGCCG 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 781, 1092
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 753
EcoT38I GRGCYC 2 cut(s) 42, 234
ErhI CCWWGG 1 cut(s) 753
FaeI CATG 4 cut(s) 31, 316, 385, 757
FaqI GGGAC 2 cut(s) 64, 924
FatI CATG 4 cut(s) 27, 312, 381, 753
FauI CCCGC 1 cut(s) 29
FauNDI CATATG 1 cut(s) 745
FbaI TGATCA 2 cut(s) 540, 1173
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 2 cut(s) 135, 361
FokI GGATG 6 cut(s) 424, 888, 906, 1022, 1071, 1118
FriOI GRGCYC 2 cut(s) 42, 234
Fsp4HI GCNGC 2 cut(s) 135, 361
FspBI CTAG 3 cut(s) 54, 852, 876
GlaI GCGC 1 cut(s) 1114
GluI GCNGC 2 cut(s) 135, 361
GsaI CCCAGC 1 cut(s) 108
GsuI CTGGAG 3 cut(s) 552, 583, 638
HaeIII GGCC 3 cut(s) 40, 64, 281
HapII CCGG 1 cut(s) 282
HhaI GCGC 1 cut(s) 1115
Hin1II CATG 4 cut(s) 31, 316, 385, 757
Hin6I GCGC 1 cut(s) 1113
HinP1I GCGC 1 cut(s) 1113
HindIII AAGCTT 1 cut(s) 1118
HinfI GANTC 5 cut(s) 180, 446, 670, 778, 1201
HpaII CCGG 1 cut(s) 282
HphI GGTGA 4 cut(s) 596, 705, 1159, 1183
Hpy166II GTNNAC 2 cut(s) 121, 741
Hpy188I TCNGA 4 cut(s) 84, 236, 838, 1139
Hpy188III TCNNGA 5 cut(s) 569, 590, 701, 775, 941
Hpy8I GTNNAC 2 cut(s) 121, 741
Hpy99I CGWCG 2 cut(s) 331, 633
HpyAV CCTTC 2 cut(s) 4, 605
HpyCH4III ACNGT 5 cut(s) 207, 291, 548, 738, 1153
HpyCH4IV ACGT 3 cut(s) 210, 628, 1209
HpyCH4V TGCA 7 cut(s) 134, 363, 377, 452, 502, 999, 1034
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 238, 282, 449, 482
HpyF3I CTNAG 3 cut(s) 233, 510, 930
HpySE526I ACGT 3 cut(s) 210, 628, 1209
Hsp92II CATG 4 cut(s) 31, 316, 385, 757
HspAI GCGC 1 cut(s) 1113
KpnI GGTACC 1 cut(s) 867
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Ksp22I TGATCA 2 cut(s) 540, 1173
Kzo9I GATC 5 cut(s) 478, 540, 697, 771, 1173
Lsp1109I GCAGC 2 cut(s) 146, 347
LweI GCATC 1 cut(s) 1191
MaeI CTAG 3 cut(s) 54, 852, 876
MaeII ACGT 3 cut(s) 210, 628, 1209
MaeIII GTNAC 3 cut(s) 602, 711, 1147
MalI GATC 5 cut(s) 480, 542, 699, 773, 1175
MboI GATC 5 cut(s) 478, 540, 697, 771, 1173
MboII GAAGA 7 cut(s) 200, 207, 329, 665, 742, 1154, 1169
MhlI GDGCHC 2 cut(s) 42, 234
MlyI GAGTC 3 cut(s) 664, 772, 1195
MmeI TCCRAC 3 cut(s) 157, 947, 1036
MnlI CCTC 9 cut(s) 75, 240, 253, 382, 662, 726, 1138, 1145, 1216
MroNI GCCGGC 1 cut(s) 281
MroXI GAANNNNTTC 2 cut(s) 197, 1230
MseI TTAA 4 cut(s) 432, 794, 831, 1206
MspA1I CMGCKG 1 cut(s) 91
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 783, 1094
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 2 cut(s) 783, 1094
MvnI CGCG 1 cut(s) 1087
MwoI GCNNNNNNNGC 5 cut(s) 110, 238, 282, 449, 482
NaeI GCCGGC 1 cut(s) 283
NcoI CCATGG 1 cut(s) 753
NdeI CATATG 1 cut(s) 745
NdeII GATC 5 cut(s) 478, 540, 697, 771, 1173
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 4 cut(s) 31, 316, 385, 757
NlaIV GGNNCC 3 cut(s) 40, 865, 940
NmeAIII GCCGAG 1 cut(s) 257
NmuCI GTSAC 3 cut(s) 602, 711, 1147
NspI RCATGY 1 cut(s) 385
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 877
PdiI GCCGGC 1 cut(s) 283
PdmI GAANNNNTTC 2 cut(s) 197, 1230
PfeI GAWTC 2 cut(s) 180, 446
PflMI CCANNNNNTGG 1 cut(s) 104
PfoI TCCNGGA 1 cut(s) 781
PkrI GCNGC 2 cut(s) 136, 362
PleI GAGTC 3 cut(s) 664, 772, 1195
PpsI GAGTC 3 cut(s) 664, 772, 1195
Ppu21I YACGTR 1 cut(s) 211
PsiI TTATAA 2 cut(s) 992, 1043
Psp124BI GAGCTC 1 cut(s) 234
Psp1406I AACGTT 1 cut(s) 1209
Psp6I CCWGG 2 cut(s) 781, 1092
PspFI CCCAGC 1 cut(s) 104
PspGI CCWGG 2 cut(s) 781, 1092
PspN4I GGNNCC 3 cut(s) 40, 865, 940
PspOMI GGGCCC 1 cut(s) 38
PspPI GGNCC 3 cut(s) 38, 39, 938
PstI CTGCAG 1 cut(s) 365
RsaI GTAC 7 cut(s) 122, 209, 251, 418, 865, 918, 1218
RsaNI GTAC 7 cut(s) 121, 208, 250, 417, 864, 917, 1217
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 4 cut(s) 432, 794, 831, 1206
SatI GCNGC 2 cut(s) 135, 361
Sau3AI GATC 5 cut(s) 478, 540, 697, 771, 1173
Sau96I GGNCC 3 cut(s) 38, 39, 938
SchI GAGTC 3 cut(s) 664, 772, 1195
ScrFI CCNGG 2 cut(s) 783, 1094
SduI GDGCHC 2 cut(s) 42, 234
SfaNI GCATC 1 cut(s) 1191
SfcI CTRYAG 4 cut(s) 298, 361, 678, 1108
SinI GGWCC 1 cut(s) 938
SmlI CTYRAG 2 cut(s) 253, 386
SmoI CTYRAG 2 cut(s) 253, 386
SsiI CCGC 6 cut(s) 36, 89, 269, 285, 371, 660
SspMI CTAG 3 cut(s) 54, 852, 876
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 781, 1092
StyI CCWWGG 1 cut(s) 753
TaaI ACNGT 5 cut(s) 207, 291, 548, 738, 1153
TaiI ACGT 3 cut(s) 213, 631, 1212
TatI WGTACW 1 cut(s) 916
TfiI GAWTC 2 cut(s) 180, 446
Tru1I TTAA 4 cut(s) 432, 794, 831, 1206
Tru9I TTAA 4 cut(s) 432, 794, 831, 1206
TscAI CASTG 2 cut(s) 210, 249
TseFI GTSAC 3 cut(s) 602, 711, 1147
TseI GCWGC 2 cut(s) 134, 360
Tsp45I GTSAC 3 cut(s) 602, 711, 1147
TspDTI ATGAA 2 cut(s) 201, 890
TspGWI ACGGA 1 cut(s) 1116
TspRI CASTG 2 cut(s) 210, 249
Van91I CCANNNNNTGG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 938
XapI RAATTY 1 cut(s) 197
XceI RCATGY 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 740
XmnI GAANNNNTTC 2 cut(s) 197, 1230
XspI CTAG 3 cut(s) 54, 852, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.