Rroxscaffold_2G00081590

Mannose-1-phosphate guanyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012792
Physical Location & Seq
Forward (+)
4659244 .. 4663278
4035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00081590.1

Sequence Viewer

Length: 1125 bp
ATGGAAAGCTCAGGAGAGAAGGTCGTCGCCGTTATCATGGTCGGAGGCCCAACTAAAGGGACTAGATTTCGGCCTCTATCATTCAACACGCCAAAGCCGCTATTCCCGTTGGCTGGGCAAGCCATGGTTCATCATCCAATCTCAGCTTGTAAGAAGATACCCAATTTGGCTCAAATTTTTCTAATTGGGTTTTATGAGGAGCGTGAGTTTGCACTATATGTTTCTTCGATTTCCAATGAGCTTAGAGTGCCGGTGAGTTTTCCACTTCCAGATATGCTTGAGGCTCATATAAAATATGGTGGAATGGGTACAATGCTAGTAATCAAGGTTTCTGCGGAATCTGCCAACGAGTTTGGTGAGTTGGTTGCTGATCCAGTCACCAAAGAACTGTTGCATTACACTGAGAAACCAGAGACTTTTGTGAGTGATTTGATCAACTGTGGTGTTTATGTATTTACTCCTGATGTTTTCAATGCCATTGAAGACGTATCCAGTCACCGGGAAGGCAGAGCTAATTTACGGCGTGTTTCCAGTTTTGAAGCTCTCCAGTCTGCTACAAGGAATCTTCCTACAGATTTTGTCAGATTGGATCAAGATATTTTGTCACCTCTTGCTGGAAAGAAGCAATTATATACTTATGAGACCATGGATTTCTGGGAACAGATCAAGACTCCAGGAATGTCATTGAAGTGTTCGTCTTTGTATCTTTCTCAATTTCGATTCACCTCTTCACATCTATTAGCTAGTGGAGATGGAATCAAGAATGCCACAATAGTTGGTGATGTCTATATTCATCCATCGGCAAAAGTACATCCTAGTGCTAAGATTGGTCCCAATGTATCTCTTTCTGCAAATGTTCGGATAGGTGCAGGAGTAAGGCTTATAAGTTGCATCGTCTTGGATGATGTGGAAATTAAGGAAAATGCAGTTGTTATACATTCTATTGTTGGATGGAAGTCATCTATTGGGAAATGGTCACGTGCTGAAGGAGACTACAATGCAAAGCTTGGAATTACCATCCTTGGAGAAGCAGTAACTGTTGAAGACGAAGTTGTGGTGATTAACAGCATTGTTCTCCCGAACAAGACTCTTAATGTTAGCGTACAGGAGGAGATCATTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 map00051 map00520 map01100 map01110
Pfam Domains
Protein Families
Hexapep NTP_transferase

Protein Analysis

374

Amino Acids

40.98

Weight (kDa)

5.89

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 10 - 67 9.7e-08 Nucleotidyl transferase
NTP_transferase PF00483 88 - 171 2.2e-09 Nucleotidyl transferase
LbH_EIF2B PF25084 254 - 325 1.6e-06 EIF2B subunit epsilon LbH domain
Hexapep PF00132 256 - 289 7.9e-06 Bacterial transferase hexapeptide (six repeats)
GMPPB_C PF25087 258 - 373 6.3e-37 GMPPB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04650 AT2G04650
fragaria_vesca FvH4_6g49920
malus_domestica MD09G1040800.v1.1 MD17G1042000.v1.1
prunus_persica Prupe.3G276900_v2.0.a1 Prupe.3G276900_v2.0.a1
pyrus_communis pycom111g03180 pycom17g03750
rosa_chinensis RchiOBHm_Chr2g0170091
rosa_laevigata RLG00000021921
rosa_multiflora Rmu_sc0013453.1_g000005
rosa_roxburghii Rroxscaffold_2G00081590
rosa_rugosa Rorug02G0545700.1
rosa_samantha Rh2AG617800 Rh2BG629300 Rh2DG641100
rosa_wichuraiana Rw2G051150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
AciI CCGC 2 cut(s) 98, 335
AclWI GGATC 2 cut(s) 365, 597
AcsI RAATTY 1 cut(s) 174
AcuI CTGAAG 1 cut(s) 1005
AcvI CACGTG 1 cut(s) 980
AfaI GTAC 3 cut(s) 310, 810, 1104
AfiI CCNNNNNNNGG 4 cut(s) 56, 113, 498, 614
AgsI TTSAA 6 cut(s) 85, 472, 482, 539, 688, 1043
AjnI CCWGG 1 cut(s) 673
AluBI AGCT 7 cut(s) 9, 146, 241, 512, 542, 743, 1006
AluI AGCT 7 cut(s) 9, 146, 241, 512, 542, 743, 1006
Alw26I GTCTC 3 cut(s) 407, 635, 984
AlwI GGATC 2 cut(s) 365, 597
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 2 cut(s) 46, 71
ApoI RAATTY 1 cut(s) 174
AspS9I GGNCC 2 cut(s) 47, 830
AsuC2I CCSGG 1 cut(s) 500
AsuHPI GGTGA 8 cut(s) 265, 368, 370, 488, 597, 715, 791, 1069
AvaII GGWCC 1 cut(s) 830
BbrPI CACGTG 1 cut(s) 980
BbsI GAAGAC 2 cut(s) 489, 1050
BccI CCATC 4 cut(s) 746, 805, 945, 1025
BceAI ACGGC 2 cut(s) 14, 536
BciT130I CCWGG 1 cut(s) 675
BciVI GTATCC 1 cut(s) 499
BclI TGATCA 1 cut(s) 432
BcnI CCSGG 1 cut(s) 500
BcoDI GTCTC 3 cut(s) 407, 635, 984
BfaI CTAG 4 cut(s) 63, 317, 744, 816
BfmI CTRYAG 1 cut(s) 570
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 1 cut(s) 98
BlsI GCNGC 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 500, 675
Bme18I GGWCC 1 cut(s) 830
BmgT120I GGNCC 2 cut(s) 47, 830
BmiI GGNNCC 1 cut(s) 832
BmrFI CCNGG 2 cut(s) 500, 675
BmsI GCATC 1 cut(s) 900
BpiI GAAGAC 2 cut(s) 489, 1050
BpmI CTGGAG 2 cut(s) 530, 657
Bpu10I CCTNAGC 1 cut(s) 10
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 500
BsaAI YACGTR 1 cut(s) 980
BsaI GGTCTC 1 cut(s) 635
BsaJI CCNNGG 3 cut(s) 123, 645, 1021
BsaXI ACNNNNNCTCC 6 cut(s) 6, 36, 191, 221, 1017, 1047
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 113, 498, 614
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 4 cut(s) 374, 492, 531, 547
BseBI CCWGG 1 cut(s) 675
BseDI CCNNGG 3 cut(s) 123, 645, 1021
BseGI GGATG 6 cut(s) 133, 793, 811, 907, 956, 1017
BseLI CCNNNNNNNGG 4 cut(s) 56, 113, 498, 614
BseMII CTCAG 3 cut(s) 24, 156, 393
BseNI ACTGG 4 cut(s) 374, 492, 531, 547
BseRI GAGGAG 2 cut(s) 212, 1124
BseYI CCCAGC 1 cut(s) 113
BsgI GTGCAG 1 cut(s) 888
BshFI GGCC 2 cut(s) 48, 73
BsiSI CCGG 2 cut(s) 251, 499
BslFI GGGAC 2 cut(s) 73, 816
BslI CCNNNNNNNGG 4 cut(s) 56, 113, 498, 614
BsmAI GTCTC 3 cut(s) 407, 635, 984
BsmFI GGGAC 2 cut(s) 73, 816
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 2 cut(s) 48, 73
Bso31I GGTCTC 1 cut(s) 635
Bsp143I GATC 5 cut(s) 370, 432, 589, 663, 1113
Bsp19I CCATGG 2 cut(s) 123, 645
BspACI CCGC 2 cut(s) 98, 335
BspANI GGCC 2 cut(s) 48, 73
BspCNI CTCAG 3 cut(s) 23, 155, 394
BspLI GGNNCC 1 cut(s) 832
BspPI GGATC 2 cut(s) 365, 597
BspTNI GGTCTC 1 cut(s) 635
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 4 cut(s) 374, 492, 531, 547
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 123, 645, 1021
BssMI GATC 5 cut(s) 370, 432, 589, 663, 1113
BssT1I CCWWGG 3 cut(s) 123, 645, 1021
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 3 cut(s) 390, 440, 1039
Bst6I CTCTTC 1 cut(s) 733
BstBAI YACGTR 1 cut(s) 980
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 5 cut(s) 10, 142, 242, 402, 822
BstDSI CCRYGG 2 cut(s) 123, 645
BstF5I GGATG 6 cut(s) 133, 793, 811, 907, 956, 1017
BstKTI GATC 5 cut(s) 373, 435, 592, 666, 1116
BstMAI GTCTC 3 cut(s) 407, 635, 984
BstMBI GATC 5 cut(s) 370, 432, 589, 663, 1113
BstMWI GCNNNNNNNGC 4 cut(s) 97, 119, 247, 341
BstNI CCWGG 1 cut(s) 675
BstSCI CCNGG 2 cut(s) 498, 673
BstSFI CTRYAG 1 cut(s) 570
BstV2I GAAGAC 2 cut(s) 489, 1050
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 2 cut(s) 48, 73
BtgI CCRYGG 2 cut(s) 123, 645
BtsCI GGATG 6 cut(s) 133, 793, 811, 907, 956, 1017
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 1 cut(s) 120
CaiI CAGNNNCTG 1 cut(s) 1037
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 2 cut(s) 47, 830
Csp6I GTAC 3 cut(s) 309, 809, 1103
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 3 cut(s) 37, 124, 646
CviQI GTAC 3 cut(s) 309, 809, 1103
DdeI CTNAG 5 cut(s) 10, 142, 242, 402, 822
DpnI GATC 5 cut(s) 372, 434, 591, 665, 1115
DpnII GATC 5 cut(s) 370, 432, 589, 663, 1113
Eam1104I CTCTTC 1 cut(s) 733
EarI CTCTTC 1 cut(s) 733
Eco130I CCWWGG 3 cut(s) 123, 645, 1021
Eco31I GGTCTC 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 830
Eco57I CTGAAG 1 cut(s) 1005
Eco72I CACGTG 1 cut(s) 980
EcoRII CCWGG 1 cut(s) 673
EcoT14I CCWWGG 3 cut(s) 123, 645, 1021
ErhI CCWWGG 3 cut(s) 123, 645, 1021
FaeI CATG 3 cut(s) 40, 127, 649
FaqI GGGAC 2 cut(s) 73, 816
FatI CATG 3 cut(s) 36, 123, 645
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 1 cut(s) 98
FokI GGATG 6 cut(s) 120, 780, 798, 914, 963, 1004
Fsp4HI GCNGC 1 cut(s) 98
FspBI CTAG 4 cut(s) 63, 317, 744, 816
GluI GCNGC 1 cut(s) 98
GsaI CCCAGC 1 cut(s) 117
GsuI CTGGAG 2 cut(s) 530, 657
HaeIII GGCC 2 cut(s) 48, 73
HapII CCGG 2 cut(s) 251, 499
Hin1II CATG 3 cut(s) 40, 127, 649
HindIII AAGCTT 1 cut(s) 1004
HinfI GANTC 6 cut(s) 338, 562, 670, 720, 756, 1087
HpaII CCGG 2 cut(s) 251, 499
HphI GGTGA 8 cut(s) 265, 368, 370, 488, 597, 715, 791, 1069
Hpy188I TCNGA 3 cut(s) 44, 584, 861
Hpy188III TCNNGA 7 cut(s) 12, 269, 461, 593, 667, 760, 1078
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 3 cut(s) 13, 497, 980
HpyCH4III ACNGT 3 cut(s) 390, 440, 1039
HpyCH4IV ACGT 2 cut(s) 486, 979
HpyCH4V TGCA 7 cut(s) 212, 394, 851, 869, 891, 926, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 119, 247, 341
HpyF3I CTNAG 5 cut(s) 10, 142, 242, 402, 822
HpySE526I ACGT 2 cut(s) 486, 979
Hsp92II CATG 3 cut(s) 40, 127, 649
Ksp22I TGATCA 1 cut(s) 432
Kzo9I GATC 5 cut(s) 370, 432, 589, 663, 1113
LmnI GCTCC 1 cut(s) 199
LweI GCATC 1 cut(s) 900
MaeI CTAG 4 cut(s) 63, 317, 744, 816
MaeII ACGT 2 cut(s) 486, 979
MaeIII GTNAC 5 cut(s) 376, 494, 603, 975, 1033
MalI GATC 5 cut(s) 372, 434, 591, 665, 1115
MboI GATC 5 cut(s) 370, 432, 589, 663, 1113
MboII GAAGA 6 cut(s) 166, 216, 494, 557, 720, 1055
MluCI AATT 8 cut(s) 163, 174, 183, 514, 626, 713, 912, 1011
MlyI GAGTC 2 cut(s) 664, 1081
MmeI TCCRAC 2 cut(s) 22, 928
MnlI CCTC 7 cut(s) 38, 84, 190, 274, 618, 736, 1102
MseI TTAA 3 cut(s) 915, 1062, 1092
MslI CAYNNNNRTG 2 cut(s) 688, 816
MspI CCGG 2 cut(s) 251, 499
MspR9I CCNGG 2 cut(s) 500, 675
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 1 cut(s) 675
MwoI GCNNNNNNNGC 4 cut(s) 97, 119, 247, 341
NciI CCSGG 1 cut(s) 500
NcoI CCATGG 2 cut(s) 123, 645
NdeII GATC 5 cut(s) 370, 432, 589, 663, 1113
NlaIII CATG 3 cut(s) 40, 127, 649
NlaIV GGNNCC 1 cut(s) 832
NmuCI GTSAC 4 cut(s) 376, 494, 603, 975
PctI GAATGC 1 cut(s) 769
PfeI GAWTC 4 cut(s) 338, 562, 720, 756
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 1 cut(s) 99
PleI GAGTC 2 cut(s) 664, 1081
PmaCI CACGTG 1 cut(s) 980
PmlI CACGTG 1 cut(s) 980
PpsI GAGTC 2 cut(s) 664, 1081
Ppu21I YACGTR 1 cut(s) 980
PsiI TTATAA 1 cut(s) 884
Psp6I CCWGG 1 cut(s) 673
PspCI CACGTG 1 cut(s) 980
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 1 cut(s) 832
PspPI GGNCC 2 cut(s) 47, 830
PstNI CAGNNNCTG 1 cut(s) 1037
RsaI GTAC 3 cut(s) 310, 810, 1104
RsaNI GTAC 3 cut(s) 309, 809, 1103
RseI CAYNNNNRTG 2 cut(s) 688, 816
SaqAI TTAA 3 cut(s) 915, 1062, 1092
SatI GCNGC 1 cut(s) 98
Sau3AI GATC 5 cut(s) 370, 432, 589, 663, 1113
Sau96I GGNCC 2 cut(s) 47, 830
SchI GAGTC 2 cut(s) 664, 1081
ScrFI CCNGG 2 cut(s) 500, 675
SfaNI GCATC 1 cut(s) 900
SfcI CTRYAG 1 cut(s) 570
SinI GGWCC 1 cut(s) 830
SmiMI CAYNNNNRTG 2 cut(s) 688, 816
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 8 cut(s) 163, 174, 183, 514, 626, 713, 912, 1011
SsiI CCGC 2 cut(s) 98, 335
SspMI CTAG 4 cut(s) 63, 317, 744, 816
StyD4I CCNGG 2 cut(s) 498, 673
StyI CCWWGG 3 cut(s) 123, 645, 1021
TaaI ACNGT 3 cut(s) 390, 440, 1039
TaiI ACGT 2 cut(s) 489, 982
TaqI TCGA 2 cut(s) 227, 718
TasI AATT 8 cut(s) 163, 174, 183, 514, 626, 713, 912, 1011
TatI WGTACW 1 cut(s) 808
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 4 cut(s) 338, 562, 720, 756
Tru1I TTAA 3 cut(s) 915, 1062, 1092
Tru9I TTAA 3 cut(s) 915, 1062, 1092
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 4 cut(s) 376, 494, 603, 975
Tsp45I GTSAC 4 cut(s) 376, 494, 603, 975
TspDTI ATGAA 2 cut(s) 119, 782
TspRI CASTG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 830
XapI RAATTY 1 cut(s) 174
XspI CTAG 4 cut(s) 63, 317, 744, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.