Rh2BG629300

Mannose-1-phosphate guanyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012792
Physical Location & Seq
Reverse (-)
85129880 .. 85133582
3703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG629300.1

Sequence Viewer

Length: 1248 bp
ATGGAAAGCTCAGGAGAGAAGGTCGTCGCCGTAATCATGGTCGGAGGCCCAACTAAAGGGACTAGATTTCGGCCTCTGTCATTCAACACGCCAAAGCCGCTATTCCCATTGGCTGGGCAAGCCATGGTTCATCATCCAATCTCAGCTTGTAAGAAGATACCCAATTTGGCTCAAATTTTTCTAATTGGGTTTTATGAGGAGCGTGAGTTTGCACTCTATGTTTCTTCGATTTCCAATGAGCTTAGAGTGCCGGTGAGGTACTTGAAAGAGGATAAACCACATGGCTCAGCTGGTGGTATTTATTACTTCAGAGATTTGATTATGGAAGACGGCCCGTCGCATATCTTTTTGCTGAATTGTGATGTATGCTGCAGTTTTCCGCTTCCAGATATGCTTGAGGCTCATATAAAATACGGTGGAATGGGTACAATGCTAGTAATCAAGGTTTCTGCGGAATCTGCCAACGAGTTTGGTGAGTTGGTTGCTGATCCAGTCACCAAAGAACTGTTGCATTACACTGAGAAACCAGAGACTTTTGTGAGTGATTTGATCAACTGCGGTGTTTATGTATTTACCCCTGATGTTTTCAATGCCATTGAAGACGTATCCAGTCACCGGGAAGGCAGAGCTAATTTACGCCGTGTTTCCAGCTTTGAAGCGCTCCAGTCTGCTACAAGGAATCTTCCTACAGATTTTGTCAGATTGGATCAAGATATTTTGTCACCTCTTGCTGGAAAGAAGCAATTATATACTTATGAGACCATGGATTTCTGGGAACAGATTAAGACTCCAGGAATGTCATTGAAGTGTTCGTCTTTGTATCTTTCTCAATTTCGATTCACCTCTTCACATCTATTAGCTAGCGGAGATGGAATCAAGAATGCCACAATAGTTGGTGATGTTTATATTCATCCATCGGCAAAAGTGCACCCTAGTGCTAAGATTGGTCCCAATGTATCTCTTTCAGCAAATGTTCGGATAGGTGCGGGAGTAAGGCTTATAAGTTGCATCGTCTTGGATGATGTGGAAATTAAGGAAAATGCAGTTGTTATACATTCTATTGTTGGATGGAAGTCATCTATTGGGAGATGGTCACGTGTCCAGGCTGAAGGAGACTACAATGCAAAGCTTGGAATTACCATCCTTGGAGAAGCAGTAACTGTTGAAGACGAAGTTGTGGTGATTAACAGCATTGTTCTCCCGAACAAGACTCTTAATGTTAGCGTACAGGAGGAGATCATTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00051 ko00520 ko01100 ko01110 map00051 map00520 map01100 map01110
Pfam Domains
Protein Families
Hexapep NTP_transferase

Protein Analysis

415

Amino Acids

45.7

Weight (kDa)

5.81

Isoelectric Point (pI)

36.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 10 - 210 6.5e-31 Nucleotidyl transferase
LbH_EIF2B PF25084 293 - 369 7.3e-07 EIF2B subunit epsilon LbH domain
Hexapep PF00132 295 - 328 1e-05 Bacterial transferase hexapeptide (six repeats)
GMPPB_C PF25087 297 - 414 5.4e-37 GMPPB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012792)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04650 AT2G04650
fragaria_vesca FvH4_6g49920
malus_domestica MD09G1040800.v1.1 MD17G1042000.v1.1
prunus_persica Prupe.3G276900_v2.0.a1 Prupe.3G276900_v2.0.a1
pyrus_communis pycom111g03180 pycom17g03750
rosa_chinensis RchiOBHm_Chr2g0170091
rosa_laevigata RLG00000021921
rosa_multiflora Rmu_sc0013453.1_g000005
rosa_roxburghii Rroxscaffold_2G00081590
rosa_rugosa Rorug02G0545700.1
rosa_samantha Rh2AG617800 Rh2BG629300 Rh2DG641100
rosa_wichuraiana Rw2G051150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AccB7I CCANNNNNTGG 1 cut(s) 113
AciI CCGC 6 cut(s) 98, 380, 452, 558, 864, 986
AclWI GGATC 2 cut(s) 482, 714
AcsI RAATTY 1 cut(s) 174
AcuI CTGAAG 2 cut(s) 292, 1128
AcvI CACGTG 1 cut(s) 1097
AfaI GTAC 3 cut(s) 260, 427, 1227
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 4 cut(s) 56, 113, 615, 731
AflIII ACRYGT 1 cut(s) 1096
AgsI TTSAA 7 cut(s) 85, 265, 589, 599, 656, 805, 1166
AhdI GACNNNNNGTC 1 cut(s) 334
AjnI CCWGG 2 cut(s) 790, 1101
AleI CACNNNNGTG 1 cut(s) 933
AluBI AGCT 8 cut(s) 9, 146, 241, 290, 629, 651, 860, 1129
AluI AGCT 8 cut(s) 9, 146, 241, 290, 629, 651, 860, 1129
Alw21I GWGCWC 1 cut(s) 930
Alw26I GTCTC 3 cut(s) 524, 752, 1107
Alw44I GTGCAC 1 cut(s) 926
AlwI GGATC 2 cut(s) 482, 714
AlwNI CAGNNNCTG 1 cut(s) 1160
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 3 cut(s) 46, 71, 331
ApaLI GTGCAC 1 cut(s) 926
ApeKI GCWGC 1 cut(s) 369
ApoI RAATTY 1 cut(s) 174
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 3 cut(s) 47, 332, 947
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 8 cut(s) 265, 485, 487, 605, 714, 832, 908, 1192
AsuNHI GCTAGC 1 cut(s) 860
AvaII GGWCC 1 cut(s) 947
BaeGI GKGCMC 1 cut(s) 930
BbrPI CACGTG 1 cut(s) 1097
BbsI GAAGAC 3 cut(s) 333, 606, 1173
Bbv12I GWGCWC 1 cut(s) 930
BbvI GCAGC 1 cut(s) 356
BccI CCATC 5 cut(s) 863, 922, 1062, 1083, 1148
BceAI ACGGC 3 cut(s) 14, 346, 624
BciT130I CCWGG 2 cut(s) 792, 1103
BciVI GTATCC 1 cut(s) 616
BclI TGATCA 1 cut(s) 549
BcnI CCSGG 1 cut(s) 617
BcoDI GTCTC 3 cut(s) 524, 752, 1107
BfaI CTAG 4 cut(s) 63, 434, 861, 933
BfmI CTRYAG 2 cut(s) 370, 687
BfoI RGCGCY 1 cut(s) 662
BfuI GTATCC 1 cut(s) 616
BisI GCNGC 2 cut(s) 98, 370
BlpI GCTNAGC 1 cut(s) 286
BlsI GCNGC 2 cut(s) 99, 371
Bme1390I CCNGG 3 cut(s) 617, 792, 1103
Bme18I GGWCC 1 cut(s) 947
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 47, 332, 947
BmiI GGNNCC 1 cut(s) 949
BmrFI CCNGG 3 cut(s) 617, 792, 1103
BmsI GCATC 1 cut(s) 1017
BmtI GCTAGC 1 cut(s) 864
BpiI GAAGAC 3 cut(s) 333, 606, 1173
BpmI CTGGAG 2 cut(s) 647, 774
Bpu10I CCTNAGC 1 cut(s) 10
Bpu1102I GCTNAGC 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 617
BsaAI YACGTR 1 cut(s) 1097
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 3 cut(s) 123, 762, 1144
BsaXI ACNNNNNCTCC 6 cut(s) 6, 36, 191, 221, 1140, 1170
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 113, 615, 731
Bse118I RCCGGY 1 cut(s) 250
Bse1I ACTGG 3 cut(s) 491, 609, 664
BseBI CCWGG 2 cut(s) 792, 1103
BseDI CCNNGG 3 cut(s) 123, 762, 1144
BseGI GGATG 5 cut(s) 133, 910, 1024, 1073, 1140
BseLI CCNNNNNNNGG 4 cut(s) 56, 113, 615, 731
BseMII CTCAG 4 cut(s) 24, 156, 300, 510
BseNI ACTGG 3 cut(s) 491, 609, 664
BseRI GAGGAG 2 cut(s) 212, 1247
BseSI GKGCMC 1 cut(s) 930
BseXI GCAGC 1 cut(s) 356
BseYI CCCAGC 1 cut(s) 113
BshFI GGCC 3 cut(s) 48, 73, 333
BsiHKAI GWGCWC 1 cut(s) 930
BsiSI CCGG 2 cut(s) 251, 616
BslFI GGGAC 2 cut(s) 73, 933
BslI CCNNNNNNNGG 4 cut(s) 56, 113, 615, 731
BsmAI GTCTC 3 cut(s) 524, 752, 1107
BsmFI GGGAC 2 cut(s) 73, 933
BsmI GAATGC 1 cut(s) 886
BsnI GGCC 3 cut(s) 48, 73, 333
Bso31I GGTCTC 1 cut(s) 752
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 4 cut(s) 487, 549, 706, 1236
Bsp1720I GCTNAGC 1 cut(s) 286
Bsp19I CCATGG 2 cut(s) 123, 762
BspACI CCGC 6 cut(s) 98, 380, 452, 558, 864, 986
BspANI GGCC 3 cut(s) 48, 73, 333
BspCNI CTCAG 4 cut(s) 23, 155, 299, 511
BspLI GGNNCC 1 cut(s) 949
BspMAI CTGCAG 1 cut(s) 374
BspOI GCTAGC 1 cut(s) 864
BspPI GGATC 2 cut(s) 482, 714
BspTNI GGTCTC 1 cut(s) 752
BsrFI RCCGGY 1 cut(s) 250
BsrI ACTGG 3 cut(s) 491, 609, 664
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 3 cut(s) 123, 762, 1144
BssMI GATC 4 cut(s) 487, 549, 706, 1236
BssT1I CCWWGG 3 cut(s) 123, 762, 1144
Bst2UI CCWGG 2 cut(s) 792, 1103
Bst4CI ACNGT 3 cut(s) 416, 507, 1162
Bst6I CTCTTC 1 cut(s) 850
BstBAI YACGTR 1 cut(s) 1097
BstC8I GCNNGC 2 cut(s) 120, 862
BstDEI CTNAG 6 cut(s) 10, 142, 242, 286, 519, 939
BstDSI CCRYGG 2 cut(s) 123, 762
BstF5I GGATG 5 cut(s) 133, 910, 1024, 1073, 1140
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 4 cut(s) 490, 552, 709, 1239
BstMAI GTCTC 3 cut(s) 524, 752, 1107
BstMBI GATC 4 cut(s) 487, 549, 706, 1236
BstMWI GCNNNNNNNGC 4 cut(s) 97, 119, 247, 458
BstNI CCWGG 2 cut(s) 792, 1103
BstSCI CCNGG 3 cut(s) 615, 790, 1101
BstSFI CTRYAG 2 cut(s) 370, 687
BstSLI GKGCMC 1 cut(s) 930
BstV1I GCAGC 1 cut(s) 356
BstV2I GAAGAC 3 cut(s) 333, 606, 1173
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 3 cut(s) 48, 73, 333
BtgI CCRYGG 2 cut(s) 123, 762
BtsCI GGATG 5 cut(s) 133, 910, 1024, 1073, 1140
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 2 cut(s) 120, 862
CaiI CAGNNNCTG 1 cut(s) 1160
CfoI GCGC 1 cut(s) 661
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 3 cut(s) 47, 332, 947
Csp6I GTAC 3 cut(s) 259, 426, 1226
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviAII CATG 4 cut(s) 37, 124, 281, 763
CviQI GTAC 3 cut(s) 259, 426, 1226
DdeI CTNAG 6 cut(s) 10, 142, 242, 286, 519, 939
DpnI GATC 4 cut(s) 489, 551, 708, 1238
DpnII GATC 4 cut(s) 487, 549, 706, 1236
DriI GACNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 850
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 850
Eco130I CCWWGG 3 cut(s) 123, 762, 1144
Eco31I GGTCTC 1 cut(s) 752
Eco47I GGWCC 1 cut(s) 947
Eco47III AGCGCT 1 cut(s) 660
Eco57I CTGAAG 2 cut(s) 292, 1128
Eco72I CACGTG 1 cut(s) 1097
EcoRII CCWGG 2 cut(s) 790, 1101
EcoT14I CCWWGG 3 cut(s) 123, 762, 1144
ErhI CCWWGG 3 cut(s) 123, 762, 1144
FaeI CATG 4 cut(s) 40, 127, 284, 766
FaqI GGGAC 2 cut(s) 73, 933
FatI CATG 4 cut(s) 36, 123, 280, 762
FauI CCCGC 1 cut(s) 979
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 2 cut(s) 98, 370
FokI GGATG 5 cut(s) 120, 897, 1031, 1080, 1127
Fsp4HI GCNGC 2 cut(s) 98, 370
FspBI CTAG 4 cut(s) 63, 434, 861, 933
GlaI GCGC 1 cut(s) 660
GluI GCNGC 2 cut(s) 98, 370
GsaI CCCAGC 1 cut(s) 117
GsuI CTGGAG 2 cut(s) 647, 774
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 3 cut(s) 48, 73, 333
HapII CCGG 2 cut(s) 251, 616
HhaI GCGC 1 cut(s) 661
Hin1II CATG 4 cut(s) 40, 127, 284, 766
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 6 cut(s) 455, 679, 787, 837, 873, 1210
HpaII CCGG 2 cut(s) 251, 616
HphI GGTGA 8 cut(s) 265, 485, 487, 605, 714, 832, 908, 1192
Hpy166II GTNNAC 1 cut(s) 928
Hpy188I TCNGA 4 cut(s) 44, 311, 701, 978
Hpy188III TCNNGA 5 cut(s) 12, 386, 710, 877, 1201
Hpy8I GTNNAC 1 cut(s) 928
Hpy99I CGWCG 2 cut(s) 29, 340
HpyAV CCTTC 3 cut(s) 13, 614, 1103
HpyCH4III ACNGT 3 cut(s) 416, 507, 1162
HpyCH4IV ACGT 2 cut(s) 603, 1096
HpyCH4V TGCA 7 cut(s) 212, 372, 511, 928, 1008, 1043, 1124
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 119, 247, 458
HpyF3I CTNAG 6 cut(s) 10, 142, 242, 286, 519, 939
HpySE526I ACGT 2 cut(s) 603, 1096
Hsp92II CATG 4 cut(s) 40, 127, 284, 766
HspAI GCGC 1 cut(s) 659
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 4 cut(s) 487, 549, 706, 1236
LmnI GCTCC 2 cut(s) 199, 666
Lsp1109I GCAGC 1 cut(s) 356
LweI GCATC 1 cut(s) 1017
MaeI CTAG 4 cut(s) 63, 434, 861, 933
MaeII ACGT 2 cut(s) 603, 1096
MaeIII GTNAC 5 cut(s) 493, 611, 720, 1092, 1156
MalI GATC 4 cut(s) 489, 551, 708, 1238
MboI GATC 4 cut(s) 487, 549, 706, 1236
MboII GAAGA 7 cut(s) 166, 216, 338, 611, 674, 837, 1178
MhlI GDGCHC 1 cut(s) 930
MluCI AATT 9 cut(s) 163, 174, 183, 355, 631, 743, 830, 1029, 1134
MlyI GAGTC 2 cut(s) 781, 1204
MmeI TCCRAC 2 cut(s) 22, 1045
MnlI CCTC 9 cut(s) 38, 84, 190, 249, 262, 391, 735, 853, 1225
MseI TTAA 4 cut(s) 783, 1032, 1185, 1215
MslI CAYNNNNRTG 2 cut(s) 805, 933
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 251, 616
MspR9I CCNGG 3 cut(s) 617, 792, 1103
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 2 cut(s) 792, 1103
MwoI GCNNNNNNNGC 4 cut(s) 97, 119, 247, 458
NciI CCSGG 1 cut(s) 617
NcoI CCATGG 2 cut(s) 123, 762
NdeII GATC 4 cut(s) 487, 549, 706, 1236
NheI GCTAGC 1 cut(s) 860
NlaIII CATG 4 cut(s) 40, 127, 284, 766
NlaIV GGNNCC 1 cut(s) 949
NmuCI GTSAC 4 cut(s) 493, 611, 720, 1092
OliI CACNNNNGTG 1 cut(s) 933
PctI GAATGC 1 cut(s) 886
PfeI GAWTC 4 cut(s) 455, 679, 837, 873
PflMI CCANNNNNTGG 1 cut(s) 113
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 2 cut(s) 99, 371
PleI GAGTC 2 cut(s) 781, 1204
PmaCI CACGTG 1 cut(s) 1097
PmlI CACGTG 1 cut(s) 1097
PpsI GAGTC 2 cut(s) 781, 1204
Ppu21I YACGTR 1 cut(s) 1097
PsiI TTATAA 1 cut(s) 1001
Psp6I CCWGG 2 cut(s) 790, 1101
PspCI CACGTG 1 cut(s) 1097
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 2 cut(s) 790, 1101
PspN4I GGNNCC 1 cut(s) 949
PspPI GGNCC 3 cut(s) 47, 332, 947
PstI CTGCAG 1 cut(s) 374
PstNI CAGNNNCTG 1 cut(s) 1160
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 3 cut(s) 260, 427, 1227
RsaNI GTAC 3 cut(s) 259, 426, 1226
RseI CAYNNNNRTG 2 cut(s) 805, 933
SaqAI TTAA 4 cut(s) 783, 1032, 1185, 1215
SatI GCNGC 2 cut(s) 98, 370
Sau3AI GATC 4 cut(s) 487, 549, 706, 1236
Sau96I GGNCC 3 cut(s) 47, 332, 947
SchI GAGTC 2 cut(s) 781, 1204
ScrFI CCNGG 3 cut(s) 617, 792, 1103
SduI GDGCHC 1 cut(s) 930
SfaNI GCATC 1 cut(s) 1017
SfcI CTRYAG 2 cut(s) 370, 687
SinI GGWCC 1 cut(s) 947
SmiMI CAYNNNNRTG 2 cut(s) 805, 933
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 9 cut(s) 163, 174, 183, 355, 631, 743, 830, 1029, 1134
SsiI CCGC 6 cut(s) 98, 380, 452, 558, 864, 986
SspMI CTAG 4 cut(s) 63, 434, 861, 933
StyD4I CCNGG 3 cut(s) 615, 790, 1101
StyI CCWWGG 3 cut(s) 123, 762, 1144
TaaI ACNGT 3 cut(s) 416, 507, 1162
TaiI ACGT 2 cut(s) 606, 1099
TaqI TCGA 2 cut(s) 227, 835
TasI AATT 9 cut(s) 163, 174, 183, 355, 631, 743, 830, 1029, 1134
TauI GCSGC 1 cut(s) 100
TfiI GAWTC 4 cut(s) 455, 679, 837, 873
Tru1I TTAA 4 cut(s) 783, 1032, 1185, 1215
Tru9I TTAA 4 cut(s) 783, 1032, 1185, 1215
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 4 cut(s) 493, 611, 720, 1092
TseI GCWGC 1 cut(s) 369
Tsp45I GTSAC 4 cut(s) 493, 611, 720, 1092
TspDTI ATGAA 2 cut(s) 119, 899
TspRI CASTG 1 cut(s) 523
Van91I CCANNNNNTGG 1 cut(s) 113
VneI GTGCAC 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 947
XapI RAATTY 1 cut(s) 174
XspI CTAG 4 cut(s) 63, 434, 861, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.