MD09G1047300.v1.1

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016676
Physical Location & Seq
Reverse (-)
3099049 .. 3101173
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1047300.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGATTTCTCTAACAGATGTCTACCATGTTGTGGCAGCCACTGTTCCTCTATATATTGCCATGATTTTGGCCTACCTTTCTGTGAAATGGTGGAAGCTCTTCACACCAGATCAATGCTCTGGAATTAACAAATTCGTTGCCAAATTCTCAATCCCTCTTCTGTCCTTTCAAATCATTTCTTCCAACAACCCTTACAAAATGAACCATAAACTCATCCTCTCGGACTGCCTCCAAAAACTGACTGCATTCCTGGCACTGGCAGCAGTCACTAAATTCAGTTCCTATGGCGGGTTGAATTCCATCATAACCGGCCTCTCTCTTTCGACGCTTCCTAACACGTTGATCCTCGGGATTCCGCTGCTGAAGGCCATGTACGGGGATGAGGCGGCCAAGCTTGTGACACAGATAGTTGTGTTGCAGAGCTTAATCTGGTACAATATGTTGCTGTTTCTGTTTGAGTTCAGGGCCGCTAAGGCGGCGTCTGTCACACCTTCATCAGAACCCACTGCAGAGGATATGGAGGTGCCTCACGAAGCGCAATCGAAAGATGAGACGGAGGACGAAACGAGAGCCGACAATACTAGGAAGATCAAATCTATTCTGTTAACAGTTGGGAGGAAGCTTATAACCAACCCAAACACTCATGCAACAATGTTGGGTCTGATTTGGGCAAGCATACACTACAAGTGGGGAGTGAAGCTGCCAGAAATCATTGCGCAATCAATCTCGATACTGTCGAATGGAGGACTTGGGATGGCAATGTTCAGCTTAGGCCTTTTCATGGCAACGCGCGGTAGCATAATAGCGTGTGGGACACGAAAGGCGATGCTAGCCATGGGATTGAAGTTCATAGCCGGCCCTGCGATAATGGCGCTGTCCTCCTTTGCAGTTGGACTAAGGGGCAAAGTTCTCAGAGTGGCAGTAGTGCAGGCAACTCTTCCTCAAGGAATCGTTCCTTTCGTGTTTGCGAAAGAATACAATGTACATCCAGACGTTTTGAGCACCGGGGTTATTTTTGGGATGCTCATTGCATTGCCGATAGCATTGGCCTACTACTTCCTTCTGGGATTGTAA

Protein Analysis

358

Amino Acids

38.84

Weight (kDa)

9.35

Isoelectric Point (pI)

28.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 9 - 352 4.7e-88 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016676)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15100
fragaria_vesca FvH4_6g49240
malus_domestica MD09G1047300.v1.1
prunus_persica Prupe.3G271700_v2.0.a1
pyrus_communis pycom111g03810
rosa_chinensis RchiOBHm_Chr2g0169101
rosa_laevigata RLG00000021834
rosa_roxburghii Rroxscaffold_2G00082450
rosa_rugosa Rorug02G0536900
rosa_samantha Rh2AG608800 Rh2BG621700 Rh2CG590400 Rh2DG632300
rosa_wichuraiana Rw2G050480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 626
Acc16I TGCGCA 1 cut(s) 717
AccB1I GGYRCC 1 cut(s) 523
AccB7I CCANNNNNTGG 1 cut(s) 31
AccI GTMKAC 1 cut(s) 21
AccII CGCG 2 cut(s) 790, 792
AciI CCGC 6 cut(s) 288, 356, 386, 468, 476, 792
AclWI GGATC 1 cut(s) 337
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 4 cut(s) 131, 143, 272, 295
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 3 cut(s) 374, 434, 984
AfiI CCNNNNNNNGG 5 cut(s) 31, 256, 288, 375, 781
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 3 cut(s) 170, 295, 844
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 6 cut(s) 97, 394, 423, 622, 700, 768
AluI AGCT 6 cut(s) 97, 394, 423, 622, 700, 768
Alw21I GWGCWC 1 cut(s) 1004
Alw26I GTCTC 1 cut(s) 545
AlwI GGATC 1 cut(s) 337
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 8 cut(s) 69, 310, 366, 387, 465, 772, 856, 1047
ApeKI GCWGC 4 cut(s) 35, 260, 358, 700
ApoI RAATTY 4 cut(s) 131, 143, 272, 295
Asp700I GAANNNNTTC 1 cut(s) 98
AspLEI GCGC 4 cut(s) 538, 718, 792, 874
AspS9I GGNCC 2 cut(s) 465, 857
AsuC2I CCSGG 1 cut(s) 1006
AsuNHI GCTAGC 1 cut(s) 829
AvaI CYCGRG 1 cut(s) 347
BanI GGYRCC 1 cut(s) 523
BarI GAAGNNNNNNTAC 2 cut(s) 356, 388
Bbv12I GWGCWC 1 cut(s) 1004
BbvI GCAGC 4 cut(s) 47, 272, 345, 687
BccI CCATC 2 cut(s) 308, 748
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 1 cut(s) 545
BfaI CTAG 2 cut(s) 582, 830
BfmI CTRYAG 1 cut(s) 507
BfoI RGCGCY 1 cut(s) 875
BglI GCCNNNNNGGC 1 cut(s) 473
BisI GCNGC 7 cut(s) 36, 261, 359, 387, 468, 477, 701
BlsI GCNGC 7 cut(s) 37, 262, 360, 388, 469, 478, 702
Bme1390I CCNGG 2 cut(s) 251, 1006
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 465, 857
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 2 cut(s) 251, 1006
BmsI GCATC 2 cut(s) 816, 1011
BmtI GCTAGC 1 cut(s) 833
Bpu10I CCTNAGC 2 cut(s) 471, 769
BpuEI CTTGAG 1 cut(s) 927
BpuMI CCSGG 1 cut(s) 1006
BsaHI GRCGYC 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 346, 834, 1005
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 256, 288, 375, 781
Bse118I RCCGGY 2 cut(s) 308, 854
Bse1I ACTGG 1 cut(s) 261
Bse3DI GCAATG 4 cut(s) 711, 765, 1026, 1031
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 3 cut(s) 346, 834, 1005
BseGI GGATG 5 cut(s) 213, 385, 759, 985, 1026
BseLI CCNNNNNNNGG 5 cut(s) 31, 256, 288, 375, 781
BseMI GCAATG 4 cut(s) 711, 765, 1026, 1031
BseMII CTCAG 1 cut(s) 925
BseNI ACTGG 1 cut(s) 261
BseXI GCAGC 4 cut(s) 47, 272, 345, 687
BsgI GTGCAG 1 cut(s) 947
Bsh1236I CGCG 2 cut(s) 790, 792
BshFI GGCC 8 cut(s) 71, 312, 368, 389, 467, 774, 858, 1049
BshNI GGYRCC 1 cut(s) 523
BsiHKAI GWGCWC 1 cut(s) 1004
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 3 cut(s) 309, 855, 1005
BslFI GGGAC 1 cut(s) 826
BslI CCNNNNNNNGG 5 cut(s) 31, 256, 288, 375, 781
BsmAI GTCTC 1 cut(s) 545
BsmBI CGTCTC 1 cut(s) 545
BsmFI GGGAC 1 cut(s) 826
BsmI GAATGC 1 cut(s) 245
BsnI GGCC 8 cut(s) 71, 312, 368, 389, 467, 774, 858, 1049
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 1004
Bsp1407I TGTACA 1 cut(s) 982
Bsp143I GATC 3 cut(s) 109, 342, 588
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 6 cut(s) 288, 356, 386, 468, 476, 792
BspANI GGCC 8 cut(s) 71, 312, 368, 389, 467, 774, 858, 1049
BspCNI CTCAG 1 cut(s) 924
BspFNI CGCG 2 cut(s) 790, 792
BspLI GGNNCC 1 cut(s) 525
BspMAI CTGCAG 1 cut(s) 511
BspOI GCTAGC 1 cut(s) 833
BspPI GGATC 1 cut(s) 337
BspQI GCTCTTC 1 cut(s) 104
BspT107I GGYRCC 1 cut(s) 523
BsrDI GCAATG 4 cut(s) 711, 765, 1026, 1031
BsrFI RCCGGY 2 cut(s) 308, 854
BsrGI TGTACA 1 cut(s) 982
BsrI ACTGG 1 cut(s) 261
BssAI RCCGGY 2 cut(s) 308, 854
BssECI CCNNGG 3 cut(s) 346, 834, 1005
BssMI GATC 3 cut(s) 109, 342, 588
BssNI GRCGYC 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 834
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 3 cut(s) 43, 610, 735
Bst6I CTCTTC 3 cut(s) 104, 162, 942
BstACI GRCGYC 1 cut(s) 479
BstAUI TGTACA 1 cut(s) 982
BstC8I GCNNGC 4 cut(s) 673, 831, 856, 930
BstDEI CTNAG 4 cut(s) 471, 769, 896, 911
BstDSI CCRYGG 1 cut(s) 834
BstF5I GGATG 5 cut(s) 213, 385, 759, 985, 1026
BstFNI CGCG 2 cut(s) 790, 792
BstH2I RGCGCY 1 cut(s) 875
BstHHI GCGC 4 cut(s) 538, 718, 792, 874
BstKTI GATC 3 cut(s) 112, 345, 591
BstMAI GTCTC 1 cut(s) 545
BstMBI GATC 3 cut(s) 109, 342, 588
BstMWI GCNNNNNNNGC 7 cut(s) 251, 260, 473, 476, 830, 860, 869
BstNI CCWGG 1 cut(s) 251
BstSCI CCNGG 2 cut(s) 249, 1004
BstSFI CTRYAG 1 cut(s) 507
BstUI CGCG 2 cut(s) 790, 792
BstV1I GCAGC 4 cut(s) 47, 272, 345, 687
BstXI CCANNNNNNTGG 1 cut(s) 67
BsuRI GGCC 8 cut(s) 71, 312, 368, 389, 467, 774, 858, 1049
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 839
BtsCI GGATG 5 cut(s) 213, 385, 759, 985, 1026
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 3 cut(s) 39, 254, 504
Cac8I GCNNGC 4 cut(s) 673, 831, 856, 930
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 4 cut(s) 538, 718, 792, 874
Cfr10I RCCGGY 2 cut(s) 308, 854
Cfr13I GGNCC 2 cut(s) 465, 857
CseI GACGC 2 cut(s) 334, 468
Csp6I GTAC 3 cut(s) 373, 433, 983
CviAII CATG 6 cut(s) 26, 61, 370, 644, 781, 835
CviQI GTAC 3 cut(s) 373, 433, 983
DdeI CTNAG 4 cut(s) 471, 769, 896, 911
DpnI GATC 3 cut(s) 111, 344, 590
DpnII GATC 3 cut(s) 109, 342, 588
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 3 cut(s) 104, 162, 942
EarI CTCTTC 3 cut(s) 104, 162, 942
Eco130I CCWWGG 1 cut(s) 834
Eco147I AGGCCT 1 cut(s) 774
Eco57I CTGAAG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 347
EcoRI GAATTC 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 834
Esp3I CGTCTC 1 cut(s) 545
FaeI CATG 6 cut(s) 29, 64, 373, 647, 784, 838
FaqI GGGAC 1 cut(s) 826
FatI CATG 6 cut(s) 25, 60, 369, 643, 780, 834
FauI CCCGC 1 cut(s) 281
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 7 cut(s) 36, 261, 359, 387, 468, 477, 701
FokI GGATG 5 cut(s) 200, 392, 766, 972, 1033
Fsp4HI GCNGC 7 cut(s) 36, 261, 359, 387, 468, 477, 701
FspBI CTAG 2 cut(s) 582, 830
FspI TGCGCA 1 cut(s) 717
GlaI GCGC 4 cut(s) 537, 717, 791, 873
GluI GCNGC 7 cut(s) 36, 261, 359, 387, 468, 477, 701
HaeII RGCGCY 1 cut(s) 875
HaeIII GGCC 8 cut(s) 71, 312, 368, 389, 467, 774, 858, 1049
HapII CCGG 3 cut(s) 309, 855, 1005
HgaI GACGC 2 cut(s) 334, 468
HhaI GCGC 4 cut(s) 538, 718, 792, 874
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 6 cut(s) 29, 64, 373, 647, 784, 838
Hin6I GCGC 4 cut(s) 536, 716, 790, 872
HinP1I GCGC 4 cut(s) 536, 716, 790, 872
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 2 cut(s) 392, 620
HinfI GANTC 2 cut(s) 352, 948
HpaI GTTAAC 1 cut(s) 606
HpaII CCGG 3 cut(s) 309, 855, 1005
Hpy166II GTNNAC 2 cut(s) 22, 606
Hpy188I TCNGA 4 cut(s) 223, 499, 663, 914
Hpy188III TCNNGA 5 cut(s) 120, 349, 530, 727, 989
Hpy8I GTNNAC 2 cut(s) 22, 606
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 3 cut(s) 358, 501, 1070
HpyCH4III ACNGT 3 cut(s) 43, 610, 735
HpyCH4IV ACGT 2 cut(s) 338, 993
HpyCH4V TGCA 7 cut(s) 245, 418, 509, 647, 887, 928, 1031
HpyF10VI GCNNNNNNNGC 7 cut(s) 251, 260, 473, 476, 830, 860, 869
HpyF3I CTNAG 4 cut(s) 471, 769, 896, 911
HpySE526I ACGT 2 cut(s) 338, 993
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 6 cut(s) 29, 64, 373, 647, 784, 838
HspAI GCGC 4 cut(s) 536, 716, 790, 872
KroI GCCGGC 1 cut(s) 854
KroNI GCCGGC 1 cut(s) 856
KspAI GTTAAC 1 cut(s) 606
Kzo9I GATC 3 cut(s) 109, 342, 588
LguI GCTCTTC 1 cut(s) 104
Lsp1109I GCAGC 4 cut(s) 47, 272, 345, 687
LweI GCATC 2 cut(s) 816, 1011
MaeI CTAG 2 cut(s) 582, 830
MaeII ACGT 2 cut(s) 338, 993
MaeIII GTNAC 3 cut(s) 265, 397, 484
MalI GATC 3 cut(s) 111, 344, 590
MboI GATC 3 cut(s) 109, 342, 588
MboII GAAGA 5 cut(s) 91, 149, 171, 598, 929
MhlI GDGCHC 1 cut(s) 1004
MluCI AATT 5 cut(s) 123, 131, 143, 272, 295
MmeI TCCRAC 2 cut(s) 207, 871
MroNI GCCGGC 1 cut(s) 854
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 3 cut(s) 126, 425, 605
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 3 cut(s) 309, 855, 1005
MspR9I CCNGG 2 cut(s) 251, 1006
Mva1269I GAATGC 1 cut(s) 245
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 2 cut(s) 790, 792
MwoI GCNNNNNNNGC 7 cut(s) 251, 260, 473, 476, 830, 860, 869
NaeI GCCGGC 1 cut(s) 856
NciI CCSGG 1 cut(s) 1006
NcoI CCATGG 1 cut(s) 834
NdeII GATC 3 cut(s) 109, 342, 588
NgoMIV GCCGGC 1 cut(s) 854
NheI GCTAGC 1 cut(s) 829
NlaIII CATG 6 cut(s) 29, 64, 373, 647, 784, 838
NlaIV GGNNCC 1 cut(s) 525
NmuCI GTSAC 3 cut(s) 265, 397, 484
NsbI TGCGCA 1 cut(s) 717
PceI AGGCCT 1 cut(s) 774
PciSI GCTCTTC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 2 cut(s) 734, 957
PctI GAATGC 1 cut(s) 245
PdiI GCCGGC 1 cut(s) 856
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 2 cut(s) 352, 948
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 7 cut(s) 37, 262, 360, 388, 469, 478, 702
PsiI TTATAA 1 cut(s) 626
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 2 cut(s) 465, 857
PstI CTGCAG 1 cut(s) 511
PstNI CAGNNNCTG 1 cut(s) 41
RsaI GTAC 3 cut(s) 374, 434, 984
RsaNI GTAC 3 cut(s) 373, 433, 983
SapI GCTCTTC 1 cut(s) 104
SaqAI TTAA 3 cut(s) 126, 425, 605
SatI GCNGC 7 cut(s) 36, 261, 359, 387, 468, 477, 701
Sau3AI GATC 3 cut(s) 109, 342, 588
Sau96I GGNCC 2 cut(s) 465, 857
ScrFI CCNGG 2 cut(s) 251, 1006
SduI GDGCHC 1 cut(s) 1004
SfaNI GCATC 2 cut(s) 816, 1011
SfcI CTRYAG 1 cut(s) 507
SmlI CTYRAG 1 cut(s) 942
SmoI CTYRAG 1 cut(s) 942
Sse9I AATT 5 cut(s) 123, 131, 143, 272, 295
SseBI AGGCCT 1 cut(s) 774
SsiI CCGC 6 cut(s) 288, 356, 386, 468, 476, 792
SspMI CTAG 2 cut(s) 582, 830
StuI AGGCCT 1 cut(s) 774
StyD4I CCNGG 2 cut(s) 249, 1004
StyI CCWWGG 1 cut(s) 834
TaaI ACNGT 3 cut(s) 43, 610, 735
TaiI ACGT 2 cut(s) 341, 996
TaqI TCGA 4 cut(s) 323, 542, 728, 737
TasI AATT 5 cut(s) 123, 131, 143, 272, 295
TatI WGTACW 1 cut(s) 982
TauI GCSGC 3 cut(s) 389, 470, 479
TfiI GAWTC 2 cut(s) 352, 948
Tru1I TTAA 3 cut(s) 126, 425, 605
Tru9I TTAA 3 cut(s) 126, 425, 605
TscAI CASTG 3 cut(s) 46, 261, 511
TseFI GTSAC 3 cut(s) 265, 397, 484
TseI GCWGC 4 cut(s) 35, 260, 358, 700
Tsp45I GTSAC 3 cut(s) 265, 397, 484
TspDTI ATGAA 4 cut(s) 215, 483, 769, 838
TspGWI ACGGA 1 cut(s) 569
TspRI CASTG 3 cut(s) 46, 261, 511
Van91I CCANNNNNTGG 1 cut(s) 31
XapI RAATTY 4 cut(s) 131, 143, 272, 295
XmiI GTMKAC 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 582, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.