Rh2CG590400

May act as a component of the auxin efflux carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016676
Physical Location & Seq
Forward (+)
76704150 .. 76705979
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG590400.1

Sequence Viewer

Length: 1014 bp
ATGGGTTTAGCCTATGTTTCCGTGAAATGGTGGAAGCTTTTCACACCAGAACAATGTTCTGGCATAAACAAGTTTGTCGCGAAATTCTCAATCCCATTGCTGTCATTTCAAGTGATTTCTGCCAATAACCCCTACAAAATGAACCTCAAGCTCATTCTCTCGGATGGTCTACAAAAGGCAATTGTTTTCTTGGCACTGACAGCAATCACTAAATTCAGCACCCATGGTGGCTTGAATTCCATCATCACAGGTCTTTCTCTCTCCACCTTGCCTAACACATTGATCCTTGGAATTCCACTTTTGAAGGCCATGTATGGTGAAGAAGCAGGCGCGCTTGTTAGTCAGATAGTTGTCTTACAAAGCTTAGTTTGGTACAATCTGTTGCTGTTTCTGTTCGAGTTTGGAGCAGCAAAGGCAGCTTCTGTGACACCTTCATCAGAAGCAACTGCAGAGGAGATGGAGGCCCCTAATGAAGCACAATCGAAAGACGAAGCAGAGGATGAAGAAAGACCCAGAAATCCAAAGAAAATGAAATCTATTCTATTGACAGTTGGGAGGAAGCTTTTTGCCAACCCCAACACTCATGCAACAATGTTGAGTCTAATTTGGGCTGGCATACACTTCAGATGGGGAGTGAAAATGCCAGAAATTGTTACGCAATCAATTTCGATACTGGCAAATGGAGGACTTGGCATGGCAACATTCAGCTTAGGCCTTTTCATGGCGTCGCGGCCTAGTATAATAGCTTGCGGAACACGAATGACAGCGGTAGCCATGGGACTTAAGTTCATTGCTGGACCTGCTGTAATGGCACTGTCTTCCTTTGCAATAGGACTAAGAGGCAGAGCTCTGAGAATAGCAATTGTGCAGGCGGCTCTTCCTCAAGGAATTGTTCCTTTTGTTTTCGCCAAGGAATACAATGTGCATCCAGAAATTCTAAGCACCGCAGTAATTTTTGGCATGCTCATTGCTTTGCCTATAGCATTGGCCTACTACTTCCTACTGGGATTATAA

Protein Analysis

337

Amino Acids

36.37

Weight (kDa)

9.45

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 1 - 332 1e-86 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016676)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15100
fragaria_vesca FvH4_6g49240
malus_domestica MD09G1047300.v1.1
prunus_persica Prupe.3G271700_v2.0.a1
pyrus_communis pycom111g03810
rosa_chinensis RchiOBHm_Chr2g0169101
rosa_laevigata RLG00000021834
rosa_roxburghii Rroxscaffold_2G00082450
rosa_rugosa Rorug02G0536900
rosa_samantha Rh2AG608800 Rh2BG621700 Rh2CG590400 Rh2DG632300
rosa_wichuraiana Rw2G050480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1012
Acc36I ACCTGC 1 cut(s) 808
AccI GTMKAC 1 cut(s) 169
AccII CGCG 3 cut(s) 80, 332, 730
AciI CCGC 5 cut(s) 730, 750, 767, 872, 945
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 5 cut(s) 83, 212, 235, 291, 933
AcuI CTGAAG 1 cut(s) 607
AcyI GRCGYC 1 cut(s) 725
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 27, 721
AflII CTTAAG 1 cut(s) 782
AgsI TTSAA 3 cut(s) 110, 235, 304
AluBI AGCT 8 cut(s) 37, 151, 363, 419, 562, 708, 746, 848
AluI AGCT 8 cut(s) 37, 151, 363, 419, 562, 708, 746, 848
Alw21I GWGCWC 1 cut(s) 850
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 5 cut(s) 306, 462, 712, 731, 987
ApeKI GCWGC 2 cut(s) 407, 416
ApoI RAATTY 5 cut(s) 83, 212, 235, 291, 933
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 2 cut(s) 332, 334
AspS9I GGNCC 2 cut(s) 463, 797
AsuHPI GGTGA 1 cut(s) 329
AvaII GGWCC 1 cut(s) 797
BanII GRGCYC 1 cut(s) 850
BbsI GAAGAC 1 cut(s) 810
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 2 cut(s) 419, 428
BccI CCATC 4 cut(s) 158, 248, 451, 621
BfaI CTAG 1 cut(s) 735
BfmI CTRYAG 2 cut(s) 447, 978
BfrI CTTAAG 1 cut(s) 782
BfuAI ACCTGC 1 cut(s) 808
BisI GCNGC 4 cut(s) 408, 417, 731, 873
BlsI GCNGC 4 cut(s) 409, 418, 732, 874
Bme18I GGWCC 1 cut(s) 797
BmgT120I GGNCC 2 cut(s) 463, 797
BmiI GGNNCC 1 cut(s) 465
BmrI ACTGGG 1 cut(s) 1013
BmsI GCATC 1 cut(s) 934
BmuI ACTGGG 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 810
Bpu10I CCTNAGC 1 cut(s) 709
BpuEI CTTGAG 2 cut(s) 131, 867
BsaHI GRCGYC 1 cut(s) 725
BsaJI CCNNGG 4 cut(s) 223, 286, 774, 909
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 721
Bse1I ACTGG 2 cut(s) 678, 1008
Bse3DI GCAATG 3 cut(s) 95, 789, 966
BseDI CCNNGG 4 cut(s) 223, 286, 774, 909
BseGI GGATG 3 cut(s) 169, 505, 925
BseLI CCNNNNNNNGG 2 cut(s) 27, 721
BseMI GCAATG 3 cut(s) 95, 789, 966
BseMII CTCAG 1 cut(s) 842
BseNI ACTGG 2 cut(s) 678, 1008
BsePI GCGCGC 1 cut(s) 330
BseRI GAGGAG 1 cut(s) 467
BseXI GCAGC 2 cut(s) 419, 428
BsgI GTGCAG 1 cut(s) 887
Bsh1236I CGCG 3 cut(s) 80, 332, 730
BshFI GGCC 5 cut(s) 308, 464, 714, 733, 989
BsiHKAI GWGCWC 1 cut(s) 850
BslFI GGGAC 1 cut(s) 792
BslI CCNNNNNNNGG 2 cut(s) 27, 721
BsmFI GGGAC 1 cut(s) 792
BsnI GGCC 5 cut(s) 308, 464, 714, 733, 989
Bsp1286I GDGCHC 1 cut(s) 850
Bsp143I GATC 1 cut(s) 282
Bsp19I CCATGG 2 cut(s) 223, 774
Bsp68I TCGCGA 1 cut(s) 80
BspACI CCGC 5 cut(s) 730, 750, 767, 872, 945
BspANI GGCC 5 cut(s) 308, 464, 714, 733, 989
BspCNI CTCAG 1 cut(s) 843
BspFNI CGCG 3 cut(s) 80, 332, 730
BspLI GGNNCC 1 cut(s) 465
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 808
BspPI GGATC 1 cut(s) 277
BspQI GCTCTTC 1 cut(s) 882
BspTI CTTAAG 1 cut(s) 782
BsrDI GCAATG 3 cut(s) 95, 789, 966
BsrI ACTGG 2 cut(s) 678, 1008
BssECI CCNNGG 4 cut(s) 223, 286, 774, 909
BssHII GCGCGC 1 cut(s) 330
BssMI GATC 1 cut(s) 282
BssNI GRCGYC 1 cut(s) 725
BssT1I CCWWGG 4 cut(s) 223, 286, 774, 909
Bst4CI ACNGT 2 cut(s) 550, 816
Bst6I CTCTTC 1 cut(s) 882
BstACI GRCGYC 1 cut(s) 725
BstAFI CTTAAG 1 cut(s) 782
BstC8I GCNNGC 6 cut(s) 328, 332, 613, 748, 870, 962
BstDEI CTNAG 5 cut(s) 364, 709, 836, 851, 938
BstDSI CCRYGG 2 cut(s) 223, 774
BstF5I GGATG 3 cut(s) 169, 505, 925
BstFNI CGCG 3 cut(s) 80, 332, 730
BstHHI GCGC 2 cut(s) 332, 334
BstKTI GATC 1 cut(s) 285
BstMBI GATC 1 cut(s) 282
BstMWI GCNNNNNNNGC 5 cut(s) 200, 413, 416, 800, 809
BstNSI RCATGY 1 cut(s) 964
BstSFI CTRYAG 2 cut(s) 447, 978
BstUI CGCG 3 cut(s) 80, 332, 730
BstV1I GCAGC 2 cut(s) 419, 428
BstV2I GAAGAC 1 cut(s) 810
BsuRI GGCC 5 cut(s) 308, 464, 714, 733, 989
BtgI CCRYGG 2 cut(s) 223, 774
BtsCI GGATG 3 cut(s) 169, 505, 925
BtsIMutI CAGTG 2 cut(s) 194, 812
BtuMI TCGCGA 1 cut(s) 80
BveI ACCTGC 1 cut(s) 808
Cac8I GCNNGC 6 cut(s) 328, 332, 613, 748, 870, 962
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 2 cut(s) 332, 334
Cfr13I GGNCC 2 cut(s) 463, 797
CseI GACGC 1 cut(s) 714
Csp6I GTAC 1 cut(s) 373
CviAII CATG 7 cut(s) 224, 310, 584, 694, 721, 775, 961
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 5 cut(s) 364, 709, 836, 851, 938
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Ecl136II GAGCTC 1 cut(s) 848
Eco130I CCWWGG 4 cut(s) 223, 286, 774, 909
Eco147I AGGCCT 1 cut(s) 714
Eco24I GRGCYC 1 cut(s) 850
Eco47I GGWCC 1 cut(s) 797
Eco53kI GAGCTC 1 cut(s) 848
Eco57I CTGAAG 1 cut(s) 607
EcoICRI GAGCTC 1 cut(s) 848
EcoO109I RGGNCCY 1 cut(s) 463
EcoRI GAATTC 2 cut(s) 235, 291
EcoT14I CCWWGG 4 cut(s) 223, 286, 774, 909
EcoT38I GRGCYC 1 cut(s) 850
ErhI CCWWGG 4 cut(s) 223, 286, 774, 909
FaeI CATG 7 cut(s) 227, 313, 587, 697, 724, 778, 964
FaqI GGGAC 1 cut(s) 792
FatI CATG 7 cut(s) 223, 309, 583, 693, 720, 774, 960
FblI GTMKAC 1 cut(s) 169
Fnu4HI GCNGC 4 cut(s) 408, 417, 731, 873
FokI GGATG 3 cut(s) 176, 512, 912
FriOI GRGCYC 1 cut(s) 850
Fsp4HI GCNGC 4 cut(s) 408, 417, 731, 873
FspBI CTAG 1 cut(s) 735
GlaI GCGC 2 cut(s) 331, 333
GluI GCNGC 4 cut(s) 408, 417, 731, 873
HaeIII GGCC 5 cut(s) 308, 464, 714, 733, 989
HgaI GACGC 1 cut(s) 714
HhaI GCGC 2 cut(s) 332, 334
Hin1I GRCGYC 1 cut(s) 725
Hin1II CATG 7 cut(s) 227, 313, 587, 697, 724, 778, 964
Hin6I GCGC 2 cut(s) 330, 332
HinP1I GCGC 2 cut(s) 330, 332
HindIII AAGCTT 3 cut(s) 35, 361, 560
HinfI GANTC 1 cut(s) 598
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 5 cut(s) 163, 345, 439, 626, 852
Hpy188III TCNNGA 2 cut(s) 79, 929
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 1 cut(s) 730
HpyAV CCTTC 2 cut(s) 298, 441
HpyCH4III ACNGT 2 cut(s) 550, 816
HpyCH4V TGCA 5 cut(s) 449, 587, 827, 868, 925
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 413, 416, 800, 809
HpyF3I CTNAG 5 cut(s) 364, 709, 836, 851, 938
Hsp92I GRCGYC 1 cut(s) 725
Hsp92II CATG 7 cut(s) 227, 313, 587, 697, 724, 778, 964
HspAI GCGC 2 cut(s) 330, 332
Kzo9I GATC 1 cut(s) 282
LguI GCTCTTC 1 cut(s) 882
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 2 cut(s) 419, 428
LweI GCATC 1 cut(s) 934
MaeI CTAG 1 cut(s) 735
MaeIII GTNAC 2 cut(s) 424, 652
MalI GATC 1 cut(s) 284
MboI GATC 1 cut(s) 282
MboII GAAGA 4 cut(s) 332, 515, 810, 869
MfeI CAATTG 2 cut(s) 180, 861
MhlI GDGCHC 1 cut(s) 850
MlyI GAGTC 1 cut(s) 607
MnlI CCTC 8 cut(s) 155, 445, 454, 490, 549, 677, 833, 891
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 1 cut(s) 783
MspA1I CMGCKG 1 cut(s) 767
MspCI CTTAAG 1 cut(s) 782
MunI CAATTG 2 cut(s) 180, 861
MvnI CGCG 3 cut(s) 80, 332, 730
MwoI GCNNNNNNNGC 5 cut(s) 200, 413, 416, 800, 809
NcoI CCATGG 2 cut(s) 223, 774
NdeII GATC 1 cut(s) 282
NlaIII CATG 7 cut(s) 227, 313, 587, 697, 724, 778, 964
NlaIV GGNNCC 1 cut(s) 465
NmuCI GTSAC 1 cut(s) 424
NruI TCGCGA 1 cut(s) 80
NspI RCATGY 1 cut(s) 964
PaeI GCATGC 1 cut(s) 964
PauI GCGCGC 1 cut(s) 330
PceI AGGCCT 1 cut(s) 714
PciSI GCTCTTC 1 cut(s) 882
PdmI GAANNNNTTC 1 cut(s) 38
PkrI GCNGC 4 cut(s) 409, 418, 732, 874
PleI GAGTC 1 cut(s) 606
PpsI GAGTC 1 cut(s) 606
PsiI TTATAA 1 cut(s) 1012
Psp124BI GAGCTC 1 cut(s) 850
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 2 cut(s) 463, 797
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 422
PteI GCGCGC 1 cut(s) 330
RruI TCGCGA 1 cut(s) 80
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SacI GAGCTC 1 cut(s) 850
SapI GCTCTTC 1 cut(s) 882
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 4 cut(s) 408, 417, 731, 873
Sau3AI GATC 1 cut(s) 282
Sau96I GGNCC 2 cut(s) 463, 797
SchI GAGTC 1 cut(s) 607
SduI GDGCHC 1 cut(s) 850
SfaNI GCATC 1 cut(s) 934
SfcI CTRYAG 2 cut(s) 447, 978
SinI GGWCC 1 cut(s) 797
SmlI CTYRAG 3 cut(s) 146, 782, 882
SmoI CTYRAG 3 cut(s) 146, 782, 882
SphI GCATGC 1 cut(s) 964
SseBI AGGCCT 1 cut(s) 714
SsiI CCGC 5 cut(s) 730, 750, 767, 872, 945
SspMI CTAG 1 cut(s) 735
SstI GAGCTC 1 cut(s) 850
StuI AGGCCT 1 cut(s) 714
StyI CCWWGG 4 cut(s) 223, 286, 774, 909
TaaI ACNGT 2 cut(s) 550, 816
TaqI TCGA 3 cut(s) 396, 482, 668
TauI GCSGC 2 cut(s) 733, 875
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TscAI CASTG 2 cut(s) 201, 819
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 2 cut(s) 407, 416
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 7 cut(s) 155, 423, 486, 516, 545, 709, 778
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 2 cut(s) 201, 819
Vha464I CTTAAG 1 cut(s) 782
VpaK11BI GGWCC 1 cut(s) 797
XapI RAATTY 5 cut(s) 83, 212, 235, 291, 933
XceI RCATGY 1 cut(s) 964
XmiI GTMKAC 1 cut(s) 169
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.