MD09G1071000.v1.1

Ubiquitin fusion degradation protein UFD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011431
Physical Location & Seq
Forward (+)
4909777 .. 4912819
3043 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1071000.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGGATTTCGAGCTGAGAAGAGCGAGAGAGAAGCTAGAAAAGGAGCAGAAGGAGAGGAAAGAGAGTGCGAGGCGGAAATTGGAGAAGGAGAGGAAGGCCAAAGAGGAAGCTAAGAAGCTGAGAGAAGCCATTGACGCCGAGCGGTCCAGACGCCTCTTCGATGCCGCCCAAGCCCAACTCAAGGTTGATGAACAAATGCAAGAAAGTTTACTTGTTGGACGGGGAGTCATGTTTTACCGCGTATTTGAAGCTGTACGTTGCCAGGGTAGTGGAGATAAGATTAAGTTGCCAGCTTCCTGCTTCAACGAATTGCAAGATCAAGGTGCGTTTGATCAAGGACCCTTGTACTTTAAGTTGTCAACAGTACATCAAGGGGGTTCGTCAGAAATGATTGATGCTGATAAGGAGAAGGGTAGGAGCACCCATTCGGGCGTTTTGGAGTTCACTGCAGATGAAGGTTTTGTTGGGCTTCCTCCTCATGTATGGCAGAACTTATTTCCGACAGATACACCAAATATTCCTTTGATAGAAGTTCTCTATGTCTGGCTTTCTAAGGGGACTTATGCAAAACTTCAACCTCTTAGGGTGGGTTTTTCAGACTTGCCCAATCACAAGGCCATCCTCGAAACAAGCCTTCGTCAGCATGCCACCCTTTCTGAGGACGATATATTTACAGTCAACTACGGGGAGCTGGTATATAAGTTACAGGTCCTTGAGTTAAAACCCTCTTCAAGTGTATCTGTTCTAGAAACGGATATTGAAGTTGACATAGTTGGTGCTGATAATGATTCGGAGAAGACTCATGAAAATGTCCTAAAACCACTTATATTTGGAACATTAGAATCTGGAACGGTTGACGAAGGGCACTACACGTATTACAAGTTTACCATAGATAATGATACTTGGGAGAAAATTGCGTCGGGGGATGCAAAAGTCGAGGTGAGGATAGAAGCGGAGCCAAATTCTGGAGATACCGATATTTACATCTCCAAGCATCCTCTCATATTCCCAACAAGGCACCAGCATGAATGGTCATCCCATGACATTGGTTCGAAGGTATTGATCCTTGGCTCAAAAGACAAGAGCTTGGAAGCAGGAACTTACAGTGTTGGTGTGTATGGGTTTAAGGGAACAACCAAGTACCAAGCATCAGTGAATGTTCAAGATGACAGTAATCGGACGATGGGTCAGCAGGCTGTTTCGTCCTCATCAGCAATGGACATGGATACTGTAGAGTGTAAGAATTGTAAGCGTTATATACCCAGTCGCACAATATCGCTTCATGAAGCCTATTGTAGCAGACATAATGTTGTTTGTCAGCATGCTGGTTGCGGAGTTGTTCTCAGGGTTGAGGAGGCCAAGAATCACGTCCATTGTCCGAAATGTGAGCAAGCTTTTCATCAGGGGGAAATGGAAAAGCACATGAAAGTGTTCCATGAGCCACTTCACTGCTCCTGTGGAATTGTGCTCGAGAAGGAAGAGATGGTGCAACACCAAGCCTCCGTTTGCCCCCTTCGCCTAATCGCATGTCGATTCTGTGGGGACATGGTTCAAGCTGGAAACTCCGCTATAGATGTTCGGGACAGAATGAGAGGATTGTCTGAGCACGAGAGTGCATGTGGGTCGAGAACAGCCCCGTGCGACTCATGCGGGCGTTCTGTCATGCTGAAGGAGATGGACATCCACCGGGTTGCCGTGCATCAAAAGAACTAA

Protein Analysis

571

Amino Acids

64.11

Weight (kDa)

6.02

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFD1_N1 PF03152 89 - 173 5.6e-16 Ubiquitin fusion degradation protein UFD1, N-terminal subdomain 1
UFD1_N2 PF24842 185 - 258 8.3e-28 Ubiquitin fusion degradation protein UFD1, N-terminal subdomain 2
Znf_XAF1_N PF23580 407 - 435 9.8e-15 XIAP-associated factor 1-like, N-terminal zinc finger
TRAFD1-XIAF1_ZnF PF21366 533 - 562 2.9e-09 TRAFD1/XAF1, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15420 AT4G15420
fragaria_vesca FvH4_6g42660
malus_domestica MD09G1071000.v1.1 MD17G1101600.v1.1
prunus_persica Prupe.3G213300_v2.0.a1
pyrus_communis pycom09g03500 pycom17g09600
rosa_chinensis RchiOBHm_Chr2g0158561
rosa_laevigata RLG00000021095
rosa_multiflora Rmu_sc0017496.1_g000003 Rmu_sc0040507.1_g000001 Rmu_sc0040508.1_g000001
rosa_roxburghii Rroxscaffold_2G00091220
rosa_rugosa Rorug02G0469900
rosa_samantha Rh2AG536100 Rh2BG549200 Rh2CG519700 Rh2DG558900
rosa_wichuraiana Rw2G044380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1017
AccB7I CCANNNNNTGG 1 cut(s) 965
AccBSI CCGCTC 1 cut(s) 142
AccII CGCG 1 cut(s) 240
AciI CCGC 8 cut(s) 73, 142, 165, 238, 953, 1332, 1566, 1650
AclWI GGATC 1 cut(s) 1057
AcsI RAATTY 1 cut(s) 961
AcuI CTGAAG 1 cut(s) 1688
AcyI GRCGYC 2 cut(s) 135, 151
AfaI GTAC 4 cut(s) 255, 347, 366, 1142
AfiI CCNNNNNNNGG 3 cut(s) 181, 658, 965
AflIII ACRYGT 1 cut(s) 870
AgsI TTSAA 7 cut(s) 248, 304, 575, 732, 761, 1163, 1553
AhdI GACNNNNNGTC 2 cut(s) 224, 1185
AjiI CACGTC 1 cut(s) 1369
AjnI CCWGG 1 cut(s) 261
AjuI GAANNNNNNNTTGG 2 cut(s) 447, 479
AleI CACNNNNGTG 1 cut(s) 1611
Alw21I GWGCWC 3 cut(s) 422, 1470, 1608
AlwI GGATC 1 cut(s) 1057
Ama87I CYCGRG 1 cut(s) 1469
AoxI GGCC 3 cut(s) 96, 615, 1356
ApoI RAATTY 1 cut(s) 961
Asp700I GAANNNNTTC 1 cut(s) 1430
AspS9I GGNCC 3 cut(s) 144, 338, 709
AsuC2I CCSGG 1 cut(s) 1688
AsuHPI GGTGA 1 cut(s) 952
AsuII TTCGAA 1 cut(s) 1052
AvaI CYCGRG 1 cut(s) 1469
AvaII GGWCC 3 cut(s) 144, 338, 709
BaeGI GKGCMC 1 cut(s) 867
BanI GGYRCC 1 cut(s) 1017
BauI CACGAG 1 cut(s) 1607
BbsI GAAGAC 1 cut(s) 803
Bbv12I GWGCWC 3 cut(s) 422, 1470, 1608
BccI CCATC 4 cut(s) 626, 1177, 1477, 1669
BceAI ACGGC 1 cut(s) 1679
BcgI CGANNNNNNTGC 2 cut(s) 1605, 1639
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 1 cut(s) 1219
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 1 cut(s) 1688
BfaI CTAG 2 cut(s) 35, 746
BfmI CTRYAG 3 cut(s) 447, 1230, 1569
BfuI GTATCC 1 cut(s) 1219
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme1390I CCNGG 2 cut(s) 263, 1688
Bme18I GGWCC 3 cut(s) 144, 338, 709
BmeRI GACNNNNNGTC 2 cut(s) 224, 1185
BmeT110I CYCGRG 1 cut(s) 1469
BmgBI CACGTC 1 cut(s) 1369
BmgT120I GGNCC 3 cut(s) 144, 338, 709
BmiI GGNNCC 3 cut(s) 340, 957, 1019
BmrFI CCNGG 2 cut(s) 263, 1688
BmrI ACTGGG 1 cut(s) 1257
BmsI GCATC 6 cut(s) 151, 385, 916, 1003, 1157, 1708
BmuI ACTGGG 1 cut(s) 1257
BpiI GAAGAC 1 cut(s) 803
BplI GAGNNNNNCTC 2 cut(s) 1326, 1358
BpmI CTGGAG 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 1052
BpuEI CTTGAG 2 cut(s) 164, 734
BpuMI CCSGG 1 cut(s) 1688
BsaAI YACGTR 1 cut(s) 873
BsaBI GATNNNNATC 1 cut(s) 1679
BsaHI GRCGYC 2 cut(s) 135, 151
BsaJI CCNNGG 2 cut(s) 262, 1066
BsaXI ACNNNNNCTCC 2 cut(s) 1484, 1514
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 658, 965
Bse1I ACTGG 1 cut(s) 1263
Bse3DI GCAATG 1 cut(s) 1221
Bse8I GATNNNNATC 1 cut(s) 1679
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 262, 1066
BseGI GGATG 5 cut(s) 618, 931, 994, 1034, 1680
BseJI GATNNNNATC 1 cut(s) 1679
BseLI CCNNNNNNNGG 3 cut(s) 181, 658, 965
BseMI GCAATG 1 cut(s) 1221
BseMII CTCAG 5 cut(s) 5, 110, 648, 1357, 1593
BseNI ACTGG 1 cut(s) 1263
BseRI GAGGAG 2 cut(s) 465, 1367
BseSI GKGCMC 1 cut(s) 867
Bsh1236I CGCG 1 cut(s) 240
BshFI GGCC 3 cut(s) 98, 617, 1358
BshNI GGYRCC 1 cut(s) 1017
BsiHKAI GWGCWC 3 cut(s) 422, 1470, 1608
BsiHKCI CYCGRG 1 cut(s) 1469
BsiSI CCGG 1 cut(s) 1687
BslFI GGGAC 3 cut(s) 571, 1556, 1595
BslI CCNNNNNNNGG 3 cut(s) 181, 658, 965
BsmFI GGGAC 3 cut(s) 571, 1556, 1595
BsnI GGCC 3 cut(s) 98, 617, 1358
BsoBI CYCGRG 1 cut(s) 1469
Bsp119I TTCGAA 1 cut(s) 1052
Bsp1286I GDGCHC 4 cut(s) 422, 867, 1470, 1608
Bsp143I GATC 3 cut(s) 316, 331, 1062
BspACI CCGC 8 cut(s) 73, 142, 165, 238, 953, 1332, 1566, 1650
BspANI GGCC 3 cut(s) 98, 617, 1358
BspCNI CTCAG 5 cut(s) 6, 111, 649, 1356, 1594
BspFNI CGCG 1 cut(s) 240
BspHI TCATGA 2 cut(s) 802, 1282
BspLI GGNNCC 3 cut(s) 340, 957, 1019
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 1 cut(s) 1057
BspQI GCTCTTC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 1052
BspT107I GGYRCC 1 cut(s) 1017
BsrBI CCGCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 1221
BsrI ACTGG 1 cut(s) 1263
BssECI CCNNGG 2 cut(s) 262, 1066
BssMI GATC 3 cut(s) 316, 331, 1062
BssNI GRCGYC 2 cut(s) 135, 151
BssSI CACGAG 1 cut(s) 1607
BssT1I CCWWGG 1 cut(s) 1066
Bst2BI CACGAG 1 cut(s) 1607
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 6 cut(s) 364, 676, 853, 1106, 1172, 1231
Bst6I CTCTTC 4 cut(s) 13, 161, 733, 1473
BstACI GRCGYC 2 cut(s) 135, 151
BstBAI YACGTR 1 cut(s) 873
BstBI TTCGAA 1 cut(s) 1052
BstC8I GCNNGC 6 cut(s) 291, 645, 1194, 1323, 1392, 1652
BstDEI CTNAG 8 cut(s) 14, 111, 119, 552, 581, 657, 1343, 1602
BstENI CCTNNNNNAGG 1 cut(s) 656
BstF5I GGATG 5 cut(s) 618, 931, 994, 1034, 1680
BstFNI CGCG 1 cut(s) 240
BstKTI GATC 3 cut(s) 319, 334, 1065
BstMBI GATC 3 cut(s) 316, 331, 1062
BstMWI GCNNNNNNNGC 4 cut(s) 134, 170, 1515, 1647
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 4 cut(s) 647, 1325, 1530, 1620
BstSCI CCNGG 2 cut(s) 261, 1686
BstSFI CTRYAG 3 cut(s) 447, 1230, 1569
BstSLI GKGCMC 1 cut(s) 867
BstUI CGCG 1 cut(s) 240
BstV2I GAAGAC 1 cut(s) 803
BstXI CCANNNNNNTGG 2 cut(s) 269, 1046
BsuI GTATCC 1 cut(s) 1219
BsuRI GGCC 3 cut(s) 98, 617, 1358
BtrI CACGTC 1 cut(s) 1369
BtsCI GGATG 5 cut(s) 618, 931, 994, 1034, 1680
BtsI GCAGTG 2 cut(s) 444, 1447
BtsIMutI CAGTG 4 cut(s) 444, 1111, 1158, 1447
Cac8I GCNNGC 6 cut(s) 291, 645, 1194, 1323, 1392, 1652
CciI TCATGA 2 cut(s) 802, 1282
Cfr13I GGNCC 3 cut(s) 144, 338, 709
CseI GACGC 3 cut(s) 143, 159, 906
Csp6I GTAC 4 cut(s) 254, 346, 365, 1141
CviQI GTAC 4 cut(s) 254, 346, 365, 1141
DdeI CTNAG 8 cut(s) 14, 111, 119, 552, 581, 657, 1343, 1602
DpnI GATC 3 cut(s) 318, 333, 1064
DpnII GATC 3 cut(s) 316, 331, 1062
DriI GACNNNNNGTC 2 cut(s) 224, 1185
Eam1104I CTCTTC 4 cut(s) 13, 161, 733, 1473
Eam1105I GACNNNNNGTC 2 cut(s) 224, 1185
EarI CTCTTC 4 cut(s) 13, 161, 733, 1473
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 1066
Eco47I GGWCC 3 cut(s) 144, 338, 709
Eco57I CTGAAG 1 cut(s) 1688
Eco88I CYCGRG 1 cut(s) 1469
EcoNI CCTNNNNNAGG 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 338, 709
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 1066
ErhI CCWWGG 1 cut(s) 1066
FaqI GGGAC 3 cut(s) 571, 1556, 1595
FauI CCCGC 1 cut(s) 1643
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 5 cut(s) 605, 938, 981, 1021, 1667
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 2 cut(s) 35, 746
GluI GCNGC 1 cut(s) 165
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 3 cut(s) 98, 617, 1358
HapII CCGG 1 cut(s) 1687
HgaI GACGC 3 cut(s) 143, 159, 906
Hin1I GRCGYC 2 cut(s) 135, 151
HincII GTYRAC 4 cut(s) 360, 679, 766, 856
HindII GTYRAC 4 cut(s) 360, 679, 766, 856
HindIII AAGCTT 1 cut(s) 1392
HinfI GANTC 7 cut(s) 225, 788, 799, 842, 1363, 1533, 1643
HpaII CCGG 1 cut(s) 1687
HphI GGTGA 1 cut(s) 952
Hpy166II GTNNAC 7 cut(s) 209, 360, 444, 679, 766, 856, 885
Hpy188I TCNGA 8 cut(s) 385, 501, 598, 658, 793, 1179, 1380, 1603
Hpy8I GTNNAC 7 cut(s) 209, 360, 444, 679, 766, 856, 885
Hpy99I CGWCG 1 cut(s) 922
HpyCH4III ACNGT 6 cut(s) 364, 676, 853, 1106, 1172, 1231
HpyCH4IV ACGT 3 cut(s) 256, 872, 1368
HpyCH4V TGCA 8 cut(s) 199, 313, 449, 566, 929, 1489, 1616, 1699
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 170, 1515, 1647
HpyF3I CTNAG 8 cut(s) 14, 111, 119, 552, 581, 657, 1343, 1602
HpySE526I ACGT 3 cut(s) 256, 872, 1368
Hsp92I GRCGYC 2 cut(s) 135, 151
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 3 cut(s) 316, 331, 1062
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 5 cut(s) 43, 417, 688, 955, 1457
LweI GCATC 6 cut(s) 151, 385, 916, 1003, 1157, 1708
MaeI CTAG 2 cut(s) 35, 746
MaeII ACGT 3 cut(s) 256, 872, 1368
MaeIII GTNAC 1 cut(s) 702
MalI GATC 3 cut(s) 318, 333, 1064
MbiI CCGCTC 1 cut(s) 142
MboI GATC 3 cut(s) 316, 331, 1062
MboII GAAGA 5 cut(s) 30, 148, 720, 808, 1490
MhlI GDGCHC 4 cut(s) 422, 867, 1470, 1608
MluCI AATT 6 cut(s) 77, 308, 912, 961, 1243, 1461
MlyI GAGTC 3 cut(s) 234, 793, 1637
MmeI TCCRAC 2 cut(s) 196, 524
MroXI GAANNNNTTC 1 cut(s) 1430
MseI TTAA 4 cut(s) 282, 351, 719, 1125
MslI CAYNNNNRTG 4 cut(s) 807, 1023, 1427, 1611
MspI CCGG 1 cut(s) 1687
MspR9I CCNGG 2 cut(s) 263, 1688
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 240
MwoI GCNNNNNNNGC 4 cut(s) 134, 170, 1515, 1647
NciI CCSGG 1 cut(s) 1688
NdeII GATC 3 cut(s) 316, 331, 1062
NlaIV GGNNCC 3 cut(s) 340, 957, 1019
NmeAIII GCCGAG 1 cut(s) 163
NspI RCATGY 4 cut(s) 647, 1325, 1530, 1620
NspV TTCGAA 1 cut(s) 1052
OliI CACNNNNGTG 1 cut(s) 1611
PaeI GCATGC 2 cut(s) 647, 1325
PaeR7I CTCGAG 1 cut(s) 1469
PagI TCATGA 2 cut(s) 802, 1282
PciSI GCTCTTC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 1430
PfeI GAWTC 4 cut(s) 788, 842, 1363, 1533
PflMI CCANNNNNTGG 1 cut(s) 965
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 3 cut(s) 233, 793, 1637
PpsI GAGTC 3 cut(s) 233, 793, 1637
Ppu21I YACGTR 1 cut(s) 873
PpuMI RGGWCCY 2 cut(s) 338, 709
Psp5II RGGWCCY 2 cut(s) 338, 709
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 3 cut(s) 340, 957, 1019
PspPI GGNCC 3 cut(s) 144, 338, 709
PspPPI RGGWCCY 2 cut(s) 338, 709
PstI CTGCAG 1 cut(s) 451
RsaI GTAC 4 cut(s) 255, 347, 366, 1142
RsaNI GTAC 4 cut(s) 254, 346, 365, 1141
RseI CAYNNNNRTG 4 cut(s) 807, 1023, 1427, 1611
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 4 cut(s) 282, 351, 719, 1125
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 3 cut(s) 316, 331, 1062
Sau96I GGNCC 3 cut(s) 144, 338, 709
SchI GAGTC 3 cut(s) 234, 793, 1637
ScrFI CCNGG 2 cut(s) 263, 1688
SduI GDGCHC 4 cut(s) 422, 867, 1470, 1608
SfaNI GCATC 6 cut(s) 151, 385, 916, 1003, 1157, 1708
SfcI CTRYAG 3 cut(s) 447, 1230, 1569
Sfr274I CTCGAG 1 cut(s) 1469
SfuI TTCGAA 1 cut(s) 1052
SinI GGWCC 3 cut(s) 144, 338, 709
SlaI CTCGAG 1 cut(s) 1469
SmiMI CAYNNNNRTG 4 cut(s) 807, 1023, 1427, 1611
SmlI CTYRAG 3 cut(s) 179, 713, 1469
SmoI CTYRAG 3 cut(s) 179, 713, 1469
SphI GCATGC 2 cut(s) 647, 1325
Sse9I AATT 6 cut(s) 77, 308, 912, 961, 1243, 1461
SsiI CCGC 8 cut(s) 73, 142, 165, 238, 953, 1332, 1566, 1650
SspI AATATT 1 cut(s) 517
SspMI CTAG 2 cut(s) 35, 746
StyD4I CCNGG 2 cut(s) 261, 1686
StyI CCWWGG 1 cut(s) 1066
TaaI ACNGT 6 cut(s) 364, 676, 853, 1106, 1172, 1231
TaiI ACGT 3 cut(s) 259, 875, 1371
TaqI TCGA 8 cut(s) 9, 159, 624, 936, 1052, 1470, 1531, 1625
TasI AATT 6 cut(s) 77, 308, 912, 961, 1243, 1461
TatI WGTACW 2 cut(s) 345, 364
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 4 cut(s) 788, 842, 1363, 1533
Tru1I TTAA 4 cut(s) 282, 351, 719, 1125
Tru9I TTAA 4 cut(s) 282, 351, 719, 1125
TscAI CASTG 4 cut(s) 451, 1111, 1158, 1454
TspDTI ATGAA 8 cut(s) 204, 468, 819, 1041, 1271, 1299, 1388, 1439
TspGWI ACGGA 2 cut(s) 767, 1492
TspRI CASTG 4 cut(s) 451, 1111, 1158, 1454
Van91I CCANNNNNTGG 1 cut(s) 965
VpaK11BI GGWCC 3 cut(s) 144, 338, 709
XagI CCTNNNNNAGG 1 cut(s) 656
XapI RAATTY 1 cut(s) 961
XbaI TCTAGA 1 cut(s) 745
XceI RCATGY 4 cut(s) 647, 1325, 1530, 1620
XhoI CTCGAG 1 cut(s) 1469
XmnI GAANNNNTTC 1 cut(s) 1430
XspI CTAG 2 cut(s) 35, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.