MD17G1101600.v1.1

Ubiquitin fusion degradation protein UFD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011431
Physical Location & Seq
Reverse (-)
8628356 .. 8631501
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1101600.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGGATTTCGAGCTGAGAAGAGCGAGAGAGAAGCTGGACAAGGAGCAGAAGGAGAGGAAGCAGAGGGCGAGGCAGAAATTGGAAAGGGAGAGGAAGGCGAAAGAAGAAGCTAAGAAGCAGAGAGAAGCCATTGACGCCGAGCGATCCAGACGCCTCTTCGATGCCGCCCAAGCCCAACTTAAGGTTGATCAACAAATGCAAGAAAGTTTACTTGTCGGACGGGGAATCATGTTCTACCGTGTATTAGAAGCTGTACGTTGCCAGGGTAGTGGAGATAAGATCAAGTTGCAGGCTTCCTGCTTCAGCGAATTGCAAGATCAAAACGCGTTTGATCAGGGACCCTTGTACTTTAAATTGTCAACAGTTCATCAAGAGGGTTCTTCAGAAATGATTGATGCTGATAAGGGGAAGGGCAGGAGCACCCATTCGGGTGTTTTGGAGTTCACTGCAGACGAAGGTTTTGTTGGGCTACCTCCTCATGTATGGCAGAACTTATTTCCGGTAGATACACCAAATATTCCTTTGATAGAAGTTCGCTATGTATGGCTTCCTAAAGGGACTTACGCAAAACTTCAACCTCTTAGGGTAGGTTTTTCAGACTTGCCCAATCACAAGGCCATCCTCGAAACAAGCCTTCGTCAGCATGCCACCCTTTCTGAAGATGATATATTTACAGTCAACTACGGGGAGCTGGTCTATAAGTTACAGGTCCTTGAGTTAAAACCCTCTTCAAGTGTATCTGTTCTAGAAACAGATATTGAAGTTGACATAGTTGGTGCTGATACTGATTCGGAAAAGACTCATGAGAATGTCCTTAAACCACTTACATTTGGAACATCAGAATCTGGAACGGTTGATGAAGGGCACTATTCGTATTACAAGTTCTCCATAGATAATGATACTTGGGAGAAAATTGCTTCGGGAGACGCCAAAATTGAGGTGAAAATAGAAGCAGAGCCAAATTCTGGGGATACTGATATTTACATCTCCAAGCATCCTCTCATATTCCCAACAAGGCACCAGCATGAATGGTCTTCCCATGACATTGGTTCGAAGGTATTGATCCTTAGCTCAAAAGACAAGAGCTTGGAAGCAGGAACTTACAGTGTTGGTGTGTATGGATTTAAGGGAACAACCAAGTACCAAGTATCTGTGAATGTTCAAGGTGACAGTAATCGGACAGTAGGTCAGCAGGCCGCCTCGTCCTCATCACCAATGGACATGGATACTGTGGAGTGTAAGAATTGTAAACGTTATATACCCAGTCGGACTATAGGACTTCATGAAGCCTATTGTAGCAGACATAATGTTGTTTGTCAGCATGCTGGTTGCGGAGTTGTTCTCAGGGTTGAGGAGGCCAAGAATCATGTTCATTGTCCAAAATGTGAGCAAGCTTTTCATCATGGGGAAATGGAAAAGCACATGAAAGTGTTCCATGAGCCACTTCACTGCCCCTGCGGAATTGTGCTCGAGAAGGAAGAGATGGTGCAACACCAAGCCTCAGTTTGCCCCCTTCGCCTTATTGCGTGCCGATTCTGTGGGGACATGGTTCAAGCTGGAAGCTCCGCTATAGATGTTCGGGACAGAATGAGAGGACTGTCTGAGCACGAGAGTGCGTGCGGGTCGAGAACTGCCCCGTGCGACTCGTGCGGGCGTTCTGTCATGTTGAAGGAGATGGACATCCATCGGGTTGCCGTGCATCAAAAGAACTAA

Protein Analysis

571

Amino Acids

64.11

Weight (kDa)

6.28

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFD1_N1 PF03152 89 - 184 1.5e-14 Ubiquitin fusion degradation protein UFD1, N-terminal subdomain 1
UFD1_N2 PF24842 185 - 258 8.3e-28 Ubiquitin fusion degradation protein UFD1, N-terminal subdomain 2
Znf_XAF1_N PF23580 407 - 435 1.4e-14 XIAP-associated factor 1-like, N-terminal zinc finger
TRAFD1-XIAF1_ZnF PF21366 533 - 562 2.9e-09 TRAFD1/XAF1, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15420 AT4G15420
fragaria_vesca FvH4_6g42660
malus_domestica MD09G1071000.v1.1 MD17G1101600.v1.1
prunus_persica Prupe.3G213300_v2.0.a1
pyrus_communis pycom09g03500 pycom17g09600
rosa_chinensis RchiOBHm_Chr2g0158561
rosa_laevigata RLG00000021095
rosa_multiflora Rmu_sc0017496.1_g000003 Rmu_sc0040507.1_g000001 Rmu_sc0040508.1_g000001
rosa_roxburghii Rroxscaffold_2G00091220
rosa_rugosa Rorug02G0469900
rosa_samantha Rh2AG536100 Rh2BG549200 Rh2CG519700 Rh2DG558900
rosa_wichuraiana Rw2G044380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1017
AccB7I CCANNNNNTGG 1 cut(s) 965
AccII CGCG 1 cut(s) 326
AciI CCGC 7 cut(s) 165, 1197, 1332, 1458, 1566, 1620, 1650
AclI AACGTT 1 cut(s) 1252
AclWI GGATC 2 cut(s) 138, 1057
AcsI RAATTY 1 cut(s) 961
AcuI CTGAAG 3 cut(s) 286, 366, 678
AcyI GRCGYC 3 cut(s) 135, 151, 927
AfaI GTAC 3 cut(s) 255, 347, 1142
AfiI CCNNNNNNNGG 2 cut(s) 181, 965
AflII CTTAAG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 6 cut(s) 575, 732, 761, 1163, 1553, 1669
AhdI GACNNNNNGTC 1 cut(s) 1185
AjnI CCWGG 1 cut(s) 261
AjuI GAANNNNNNNTTGG 2 cut(s) 447, 479
AleI CACNNNNGTG 1 cut(s) 1611
Alw21I GWGCWC 3 cut(s) 422, 1470, 1608
Alw26I GTCTC 1 cut(s) 918
AlwI GGATC 2 cut(s) 138, 1057
AlwNI CAGNNNCTG 1 cut(s) 845
Ama87I CYCGRG 1 cut(s) 1469
AoxI GGCC 3 cut(s) 615, 1194, 1356
ApoI RAATTY 1 cut(s) 961
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
Asp700I GAANNNNTTC 1 cut(s) 1430
AspS9I GGNCC 2 cut(s) 338, 709
AsuHPI GGTGA 3 cut(s) 952, 1178, 1203
AsuII TTCGAA 1 cut(s) 1052
AvaI CYCGRG 1 cut(s) 1469
AvaII GGWCC 2 cut(s) 338, 709
BaeGI GKGCMC 1 cut(s) 867
BanI GGYRCC 1 cut(s) 1017
BauI CACGAG 2 cut(s) 1607, 1645
BbsI GAAGAC 1 cut(s) 1026
Bbv12I GWGCWC 3 cut(s) 422, 1470, 1608
BccI CCATC 4 cut(s) 626, 1477, 1669, 1692
BceAI ACGGC 1 cut(s) 1679
BciT130I CCWGG 1 cut(s) 263
BciVI GTATCC 2 cut(s) 964, 1219
BclI TGATCA 2 cut(s) 187, 331
BcoDI GTCTC 1 cut(s) 918
BfaI CTAG 1 cut(s) 746
BfmI CTRYAG 3 cut(s) 447, 1272, 1569
BfrI CTTAAG 1 cut(s) 179
BfuI GTATCC 2 cut(s) 964, 1219
BisI GCNGC 2 cut(s) 165, 1197
BlsI GCNGC 2 cut(s) 166, 1198
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 2 cut(s) 338, 709
BmeRI GACNNNNNGTC 1 cut(s) 1185
BmeT110I CYCGRG 1 cut(s) 1469
BmgT120I GGNCC 2 cut(s) 338, 709
BmiI GGNNCC 3 cut(s) 339, 340, 1019
BmrFI CCNGG 1 cut(s) 263
BmrI ACTGGG 1 cut(s) 1257
BmsI GCATC 4 cut(s) 151, 385, 1003, 1708
BmuI ACTGGG 1 cut(s) 1257
BpiI GAAGAC 1 cut(s) 1026
BplI GAGNNNNNCTC 2 cut(s) 1326, 1358
Bpu10I CCTNAGC 1 cut(s) 1067
Bpu14I TTCGAA 1 cut(s) 1052
BpuEI CTTGAG 1 cut(s) 734
BsaBI GATNNNNATC 1 cut(s) 1679
BsaHI GRCGYC 3 cut(s) 135, 151, 927
BsaJI CCNNGG 1 cut(s) 262
BsaWI WCCGGW 1 cut(s) 499
BsaXI ACNNNNNCTCC 2 cut(s) 869, 899
Bsc4I CCNNNNNNNGG 2 cut(s) 181, 965
Bse1I ACTGG 1 cut(s) 1263
Bse8I GATNNNNATC 1 cut(s) 1679
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 3 cut(s) 618, 994, 1680
BseJI GATNNNNATC 1 cut(s) 1679
BseLI CCNNNNNNNGG 2 cut(s) 181, 965
BseMII CTCAG 4 cut(s) 5, 1357, 1515, 1593
BseNI ACTGG 1 cut(s) 1263
BseRI GAGGAG 2 cut(s) 465, 1367
BseSI GKGCMC 1 cut(s) 867
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 3 cut(s) 617, 1196, 1358
BshNI GGYRCC 1 cut(s) 1017
BsiHKAI GWGCWC 3 cut(s) 422, 1470, 1608
BsiHKCI CYCGRG 1 cut(s) 1469
BsiSI CCGG 1 cut(s) 500
BslFI GGGAC 4 cut(s) 351, 571, 1556, 1595
BslI CCNNNNNNNGG 2 cut(s) 181, 965
BsmAI GTCTC 1 cut(s) 918
BsmBI CGTCTC 1 cut(s) 918
BsmFI GGGAC 4 cut(s) 351, 571, 1556, 1595
BsnI GGCC 3 cut(s) 617, 1196, 1358
BsoBI CYCGRG 1 cut(s) 1469
Bsp119I TTCGAA 1 cut(s) 1052
Bsp1286I GDGCHC 4 cut(s) 422, 867, 1470, 1608
Bsp143I GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
BspACI CCGC 7 cut(s) 165, 1197, 1332, 1458, 1566, 1620, 1650
BspANI GGCC 3 cut(s) 617, 1196, 1358
BspCNI CTCAG 4 cut(s) 6, 1356, 1514, 1594
BspFNI CGCG 1 cut(s) 326
BspHI TCATGA 2 cut(s) 802, 1282
BspLI GGNNCC 3 cut(s) 339, 340, 1019
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 2 cut(s) 138, 1057
BspQI GCTCTTC 1 cut(s) 13
BspT104I TTCGAA 1 cut(s) 1052
BspT107I GGYRCC 1 cut(s) 1017
BspTI CTTAAG 1 cut(s) 179
BsrI ACTGG 1 cut(s) 1263
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
BssNI GRCGYC 3 cut(s) 135, 151, 927
BssSI CACGAG 2 cut(s) 1607, 1645
Bst2BI CACGAG 2 cut(s) 1607, 1645
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 9 cut(s) 239, 364, 676, 853, 1106, 1172, 1183, 1231, 1599
Bst6I CTCTTC 4 cut(s) 13, 161, 733, 1473
BstACI GRCGYC 3 cut(s) 135, 151, 927
BstAFI CTTAAG 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 1052
BstC8I GCNNGC 8 cut(s) 291, 645, 1194, 1323, 1392, 1528, 1618, 1652
BstDEI CTNAG 7 cut(s) 14, 111, 581, 1067, 1343, 1501, 1602
BstF5I GGATG 3 cut(s) 618, 994, 1680
BstFNI CGCG 1 cut(s) 326
BstKTI GATC 6 cut(s) 146, 190, 282, 319, 334, 1065
BstMAI GTCTC 1 cut(s) 918
BstMBI GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
BstMWI GCNNNNNNNGC 4 cut(s) 134, 170, 1515, 1647
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 647, 1325
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 3 cut(s) 447, 1272, 1569
BstSLI GKGCMC 1 cut(s) 867
BstUI CGCG 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 1026
BstXI CCANNNNNNTGG 2 cut(s) 269, 1046
BsuI GTATCC 2 cut(s) 964, 1219
BsuRI GGCC 3 cut(s) 617, 1196, 1358
BtsCI GGATG 3 cut(s) 618, 994, 1680
BtsI GCAGTG 2 cut(s) 444, 1447
BtsIMutI CAGTG 3 cut(s) 444, 1111, 1447
Cac8I GCNNGC 8 cut(s) 291, 645, 1194, 1323, 1392, 1528, 1618, 1652
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 2 cut(s) 802, 1282
Cfr13I GGNCC 2 cut(s) 338, 709
CseI GACGC 3 cut(s) 143, 159, 935
Csp6I GTAC 3 cut(s) 254, 346, 1141
CviQI GTAC 3 cut(s) 254, 346, 1141
DdeI CTNAG 7 cut(s) 14, 111, 581, 1067, 1343, 1501, 1602
DpnI GATC 6 cut(s) 145, 189, 281, 318, 333, 1064
DpnII GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
DraI TTTAAA 1 cut(s) 352
DriI GACNNNNNGTC 1 cut(s) 1185
Eam1104I CTCTTC 4 cut(s) 13, 161, 733, 1473
Eam1105I GACNNNNNGTC 1 cut(s) 1185
EarI CTCTTC 4 cut(s) 13, 161, 733, 1473
Eco47I GGWCC 2 cut(s) 338, 709
Eco57I CTGAAG 3 cut(s) 286, 366, 678
Eco88I CYCGRG 1 cut(s) 1469
EcoO109I RGGNCCY 2 cut(s) 338, 709
EcoRII CCWGG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 918
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FaqI GGGAC 4 cut(s) 351, 571, 1556, 1595
FauI CCCGC 2 cut(s) 1613, 1643
FbaI TGATCA 2 cut(s) 187, 331
Fnu4HI GCNGC 2 cut(s) 165, 1197
FokI GGATG 3 cut(s) 605, 981, 1667
Fsp4HI GCNGC 2 cut(s) 165, 1197
FspBI CTAG 1 cut(s) 746
GluI GCNGC 2 cut(s) 165, 1197
HaeIII GGCC 3 cut(s) 617, 1196, 1358
HapII CCGG 1 cut(s) 500
HgaI GACGC 3 cut(s) 143, 159, 935
Hin1I GRCGYC 3 cut(s) 135, 151, 927
HincII GTYRAC 3 cut(s) 360, 679, 766
HindII GTYRAC 3 cut(s) 360, 679, 766
HindIII AAGCTT 1 cut(s) 1392
HinfI GANTC 7 cut(s) 225, 788, 799, 842, 1363, 1533, 1643
HpaII CCGG 1 cut(s) 500
HphI GGTGA 3 cut(s) 952, 1178, 1203
Hpy166II GTNNAC 6 cut(s) 209, 360, 444, 679, 766, 1250
Hpy188I TCNGA 9 cut(s) 218, 385, 598, 658, 793, 841, 1179, 1269, 1603
Hpy8I GTNNAC 6 cut(s) 209, 360, 444, 679, 766, 1250
HpyCH4III ACNGT 9 cut(s) 239, 364, 676, 853, 1106, 1172, 1183, 1231, 1599
HpyCH4IV ACGT 2 cut(s) 256, 1252
HpyCH4V TGCA 6 cut(s) 199, 289, 313, 449, 1489, 1699
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 170, 1515, 1647
HpyF3I CTNAG 7 cut(s) 14, 111, 581, 1067, 1343, 1501, 1602
HpySE526I ACGT 2 cut(s) 256, 1252
Hsp92I GRCGYC 3 cut(s) 135, 151, 927
KflI GGGWCCC 1 cut(s) 338
Ksp22I TGATCA 2 cut(s) 187, 331
Kzo9I GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 4 cut(s) 43, 417, 688, 1568
LweI GCATC 4 cut(s) 151, 385, 1003, 1708
MaeI CTAG 1 cut(s) 746
MaeII ACGT 2 cut(s) 256, 1252
MaeIII GTNAC 2 cut(s) 702, 1166
MalI GATC 6 cut(s) 145, 189, 281, 318, 333, 1064
MboI GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
MboII GAAGA 8 cut(s) 30, 116, 148, 372, 671, 720, 1026, 1490
MhlI GDGCHC 4 cut(s) 422, 867, 1470, 1608
MluCI AATT 8 cut(s) 77, 308, 353, 912, 933, 961, 1243, 1461
MluI ACGCGT 1 cut(s) 324
MlyI GAGTC 2 cut(s) 793, 1637
MmeI TCCRAC 2 cut(s) 196, 1247
MroXI GAANNNNTTC 1 cut(s) 1430
MseI TTAA 5 cut(s) 180, 351, 719, 816, 1125
MslI CAYNNNNRTG 5 cut(s) 429, 807, 1023, 1427, 1611
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 263
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 4 cut(s) 134, 170, 1515, 1647
NdeII GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
NlaIV GGNNCC 3 cut(s) 339, 340, 1019
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 1166
NspI RCATGY 2 cut(s) 647, 1325
NspV TTCGAA 1 cut(s) 1052
OliI CACNNNNGTG 1 cut(s) 1611
PaeI GCATGC 2 cut(s) 647, 1325
PaeR7I CTCGAG 1 cut(s) 1469
PagI TCATGA 2 cut(s) 802, 1282
PciSI GCTCTTC 1 cut(s) 13
PdmI GAANNNNTTC 1 cut(s) 1430
PfeI GAWTC 5 cut(s) 225, 788, 842, 1363, 1533
PflMI CCANNNNNTGG 1 cut(s) 965
PkrI GCNGC 2 cut(s) 166, 1198
PleI GAGTC 2 cut(s) 793, 1637
PpsI GAGTC 2 cut(s) 793, 1637
PpuMI RGGWCCY 2 cut(s) 338, 709
Psp1406I AACGTT 1 cut(s) 1252
Psp5II RGGWCCY 2 cut(s) 338, 709
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 3 cut(s) 339, 340, 1019
PspPI GGNCC 2 cut(s) 338, 709
PspPPI RGGWCCY 2 cut(s) 338, 709
PsrI GAACNNNNNNTAC 2 cut(s) 866, 898
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 845
RsaI GTAC 3 cut(s) 255, 347, 1142
RsaNI GTAC 3 cut(s) 254, 346, 1141
RseI CAYNNNNRTG 5 cut(s) 429, 807, 1023, 1427, 1611
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 5 cut(s) 180, 351, 719, 816, 1125
SatI GCNGC 2 cut(s) 165, 1197
Sau3AI GATC 6 cut(s) 143, 187, 279, 316, 331, 1062
Sau96I GGNCC 2 cut(s) 338, 709
SchI GAGTC 2 cut(s) 793, 1637
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 4 cut(s) 422, 867, 1470, 1608
SfaNI GCATC 4 cut(s) 151, 385, 1003, 1708
SfcI CTRYAG 3 cut(s) 447, 1272, 1569
Sfr274I CTCGAG 1 cut(s) 1469
SfuI TTCGAA 1 cut(s) 1052
SinI GGWCC 2 cut(s) 338, 709
SlaI CTCGAG 1 cut(s) 1469
SmiMI CAYNNNNRTG 5 cut(s) 429, 807, 1023, 1427, 1611
SmlI CTYRAG 3 cut(s) 179, 713, 1469
SmoI CTYRAG 3 cut(s) 179, 713, 1469
SphI GCATGC 2 cut(s) 647, 1325
Sse9I AATT 8 cut(s) 77, 308, 353, 912, 933, 961, 1243, 1461
SsiI CCGC 7 cut(s) 165, 1197, 1332, 1458, 1566, 1620, 1650
SspI AATATT 1 cut(s) 517
SspMI CTAG 1 cut(s) 746
StyD4I CCNGG 1 cut(s) 261
TaaI ACNGT 9 cut(s) 239, 364, 676, 853, 1106, 1172, 1183, 1231, 1599
TaiI ACGT 2 cut(s) 259, 1255
TaqI TCGA 6 cut(s) 9, 159, 624, 1052, 1470, 1625
TasI AATT 8 cut(s) 77, 308, 353, 912, 933, 961, 1243, 1461
TatI WGTACW 1 cut(s) 345
TauI GCSGC 2 cut(s) 167, 1199
TfiI GAWTC 5 cut(s) 225, 788, 842, 1363, 1533
Tru1I TTAA 5 cut(s) 180, 351, 719, 816, 1125
Tru9I TTAA 5 cut(s) 180, 351, 719, 816, 1125
TscAI CASTG 3 cut(s) 451, 1111, 1454
TseFI GTSAC 1 cut(s) 1166
Tsp45I GTSAC 1 cut(s) 1166
TspDTI ATGAA 8 cut(s) 356, 873, 1041, 1271, 1299, 1361, 1388, 1439
TspRI CASTG 3 cut(s) 451, 1111, 1454
Van91I CCANNNNNTGG 1 cut(s) 965
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 338, 709
XapI RAATTY 1 cut(s) 961
XbaI TCTAGA 1 cut(s) 745
XceI RCATGY 2 cut(s) 647, 1325
XhoI CTCGAG 1 cut(s) 1469
XmnI GAANNNNTTC 1 cut(s) 1430
XspI CTAG 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.