MD09G1090400.v1.1

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012257
Physical Location & Seq
Reverse (-)
6594636 .. 6598366
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1090400.v1.1.491

Sequence Viewer

Length: 972 bp
ATGATTTTGGACAAAGGGTCAATGCAATCAAACCTTGACTGCTTCCTCCATTGTACAACCCCGGTGCTCCGACCCCAATTCCTACCCAAGTCTGAGATGAGGAACCTCAATCGGCTATGGCATCCATGGGAGAGAGAAAAGGTTGAGTATTTCACATTGAGTGATCTCTGGAATTGCTATGATGAATGGAGTGCTTATGGCGCCGGAGTTCCGATCGCCATGGACAGCGGCGAGACCCTTGTGCAGTACTATGTGCCTTACCTCTCTGCTATCCAAATTTTCACTAGCAATTCATCTGTGAATAGCTTCAGGGAAGAGGCCGAGTCGGGCGACTGCGAGACTAGGGATTCGTTTAGCGATTCGTGCAGCTTTGAGAGTGAAAGTGATAAGTTGTGGAGATCGGATGGATGCTCTTCGGAAGATGGCGGGTTCGAGCAAGACAATCTCTGGCATGTGAATGATAGATTAGGGTACCGATATTTTGAGTATTTTGAGAGATCAACTCCCTATGGAAGAGTTCCTCTAATGGATAAGATCAATTCGTTAGCTCAAAGATACCCTGGCTTGATGTCATTAAGGAGTGTTGATCTCTCACCAGCTAGTTGGATGGCTGTTGCTTGGTATCCAATTTATCACATTCCAATGGGAAGGACTATCAAGGATCTGTCCACATCCTTCCTCACTTACCACACCCTTTCATCTTCTTTTCAAGACATGGACCTTGAGGATGATATTGAGAGCAACGAGCAACAGAAGCGTAAGAAAGGGGAAGGCATCTCTCTCCCTCCATTTGGTTTGGCCACTTACAAGATGCAAGGAAATGTGTGGGTATCAGGCAATTGTGGAAAGGACCAAGAGAGGCTCATGTCGCTATTGAGCGTGGCGGATTCTTGGCTAAAGCAGCTTAGGGTCAAGCACCATGACTTCAACTATTTCACAGCTATTAGGCGTGGCCAGTGCCACTACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

324

Amino Acids

37.43

Weight (kDa)

5.16

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF789 PF05623 10 - 312 2.5e-112 Protein of unknown function (DUF789)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03420
fragaria_vesca FvH4_6g45060 FvH4_6g45060
malus_domestica MD09G1090400.v1.1 MD17G1079500.v1.1
prunus_persica Prupe.3G235200_v2.0.a1 Prupe.3G235200_v2.0.a1
pyrus_communis pycom09g01510 pycom17g07850
rosa_chinensis RchiOBHm_Chr2g0162411
rosa_laevigata RLG00000021345
rosa_multiflora Rmu_sc0004020.1_g000002
rosa_roxburghii Rroxscaffold_2G00087950
rosa_rugosa Rorug02G0497800
rosa_samantha Rh2AG563500 Rh2BG576200 Rh2CG546300 Rh2DG586200
rosa_wichuraiana Rw2G046640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 471
AccB1I GGYRCC 2 cut(s) 200, 471
AciI CCGC 3 cut(s) 228, 426, 884
AclWI GGATC 1 cut(s) 669
AcoI YGGCCR 2 cut(s) 798, 952
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 3 cut(s) 55, 248, 473
AfiI CCNNNNNNNGG 1 cut(s) 791
AgsI TTSAA 2 cut(s) 710, 928
AhdI GACNNNNNGTC 1 cut(s) 16
AjnI CCWGG 1 cut(s) 559
AluBI AGCT 6 cut(s) 306, 369, 548, 599, 904, 941
AluI AGCT 6 cut(s) 306, 369, 548, 599, 904, 941
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 227, 332
AlwI GGATC 1 cut(s) 669
AoxI GGCC 3 cut(s) 318, 798, 952
ApeKI GCWGC 2 cut(s) 366, 901
ApoI RAATTY 1 cut(s) 276
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
Asp700I GAANNNNTTC 1 cut(s) 305
Asp718I GGTACC 1 cut(s) 471
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 2 cut(s) 718, 850
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 585
AvaII GGWCC 2 cut(s) 718, 850
BalI TGGCCA 2 cut(s) 800, 954
BanI GGYRCC 2 cut(s) 200, 471
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 378, 913
BccI CCATC 3 cut(s) 398, 416, 601
BciT130I CCWGG 1 cut(s) 561
BciVI GTATCC 1 cut(s) 633
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 2 cut(s) 227, 332
BfaI CTAG 3 cut(s) 285, 342, 600
BfoI RGCGCY 1 cut(s) 204
BfuI GTATCC 1 cut(s) 633
BisI GCNGC 3 cut(s) 229, 367, 902
BlsI GCNGC 3 cut(s) 230, 368, 903
BmcAI AGTACT 1 cut(s) 248
Bme1390I CCNGG 2 cut(s) 62, 561
Bme18I GGWCC 2 cut(s) 718, 850
BmeRI GACNNNNNGTC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 718, 850
BmiI GGNNCC 3 cut(s) 104, 202, 473
BmrFI CCNGG 2 cut(s) 62, 561
BmsI GCATC 4 cut(s) 130, 398, 783, 801
BplI GAGNNNNNCTC 2 cut(s) 487, 519
Bpu10I CCTNAGC 1 cut(s) 905
BpuEI CTTGAG 1 cut(s) 743
BpuMI CCSGG 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 4 cut(s) 60, 125, 219, 559
Bsc4I CCNNNNNNNGG 1 cut(s) 791
Bse1I ACTGG 1 cut(s) 955
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 4 cut(s) 60, 125, 219, 559
BseGI GGATG 6 cut(s) 121, 409, 413, 612, 671, 733
BseLI CCNNNNNNNGG 1 cut(s) 791
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 1 cut(s) 955
BseXI GCAGC 2 cut(s) 378, 913
BsgI GTGCAG 2 cut(s) 263, 385
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 3 cut(s) 320, 800, 954
BshNI GGYRCC 2 cut(s) 200, 471
BsiEI CGRYCG 1 cut(s) 216
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 2 cut(s) 62, 204
BslI CCNNNNNNNGG 1 cut(s) 791
BsmAI GTCTC 2 cut(s) 227, 332
BsnI GGCC 3 cut(s) 320, 800, 954
Bso31I GGTCTC 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 69
Bsp1407I TGTACA 1 cut(s) 53
Bsp143I GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
Bsp19I CCATGG 2 cut(s) 125, 219
BspACI CCGC 3 cut(s) 228, 426, 884
BspANI GGCC 3 cut(s) 320, 800, 954
BspCNI CTCAG 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 104, 202, 473
BspPI GGATC 1 cut(s) 669
BspQI GCTCTTC 1 cut(s) 418
BspT107I GGYRCC 2 cut(s) 200, 471
BspTNI GGTCTC 1 cut(s) 227
BsrGI TGTACA 1 cut(s) 53
BsrI ACTGG 1 cut(s) 955
BssECI CCNNGG 4 cut(s) 60, 125, 219, 559
BssMI GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 125, 219
Bst2UI CCWGG 1 cut(s) 561
Bst6I CTCTTC 3 cut(s) 309, 418, 508
BstACI GRCGYC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 93, 905
BstDSI CCRYGG 2 cut(s) 125, 219
BstF5I GGATG 6 cut(s) 121, 409, 413, 612, 671, 733
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 7 cut(s) 166, 216, 401, 500, 537, 589, 664
BstMAI GTCTC 2 cut(s) 227, 332
BstMBI GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 5 cut(s) 200, 363, 754, 868, 901
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 2 cut(s) 60, 559
BstV1I GCAGC 2 cut(s) 378, 913
BstX2I RGATCY 1 cut(s) 661
BstXI CCANNNNNNTGG 1 cut(s) 603
BstYI RGATCY 1 cut(s) 661
BsuI GTATCC 1 cut(s) 633
BsuRI GGCC 3 cut(s) 320, 800, 954
BtgI CCRYGG 2 cut(s) 125, 219
BtsCI GGATG 6 cut(s) 121, 409, 413, 612, 671, 733
BtsIMutI CAGTG 1 cut(s) 962
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 2 cut(s) 718, 850
Csp6I GTAC 3 cut(s) 54, 247, 472
CviAII CATG 6 cut(s) 126, 220, 452, 715, 865, 920
CviQI GTAC 3 cut(s) 54, 247, 472
DdeI CTNAG 2 cut(s) 93, 905
DinI GGCGCC 1 cut(s) 202
DpnI GATC 7 cut(s) 165, 215, 400, 499, 536, 588, 663
DpnII GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
DriI GACNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 2 cut(s) 798, 952
Eam1104I CTCTTC 3 cut(s) 309, 418, 508
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 3 cut(s) 309, 418, 508
EciI GGCGGA 1 cut(s) 899
Eco130I CCWWGG 2 cut(s) 125, 219
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 2 cut(s) 718, 850
Eco57I CTGAAG 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 559
EcoT14I CCWWGG 2 cut(s) 125, 219
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 125, 219
FaeI CATG 6 cut(s) 129, 223, 455, 718, 868, 923
FatI CATG 6 cut(s) 125, 219, 451, 714, 864, 919
FauI CCCGC 1 cut(s) 419
Fnu4HI GCNGC 3 cut(s) 229, 367, 902
FokI GGATG 6 cut(s) 108, 416, 420, 619, 658, 740
Fsp4HI GCNGC 3 cut(s) 229, 367, 902
FspBI CTAG 3 cut(s) 285, 342, 600
GlaI GCGC 1 cut(s) 202
GluI GCNGC 3 cut(s) 229, 367, 902
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 3 cut(s) 320, 800, 954
HapII CCGG 2 cut(s) 62, 204
HhaI GCGC 1 cut(s) 203
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 6 cut(s) 129, 223, 455, 718, 868, 923
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 4 cut(s) 323, 347, 359, 887
HpaII CCGG 2 cut(s) 62, 204
HphI GGTGA 1 cut(s) 585
Hpy166II GTNNAC 1 cut(s) 669
Hpy188I TCNGA 5 cut(s) 71, 94, 213, 403, 418
Hpy188III TCNNGA 2 cut(s) 169, 710
Hpy8I GTNNAC 1 cut(s) 669
HpyAV CCTTC 3 cut(s) 642, 685, 764
HpyCH4V TGCA 4 cut(s) 25, 244, 366, 814
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 363, 754, 868, 901
HpyF3I CTNAG 2 cut(s) 93, 905
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 6 cut(s) 129, 223, 455, 718, 868, 923
HspAI GCGC 1 cut(s) 201
KasI GGCGCC 1 cut(s) 200
KpnI GGTACC 1 cut(s) 475
Kzo9I GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
LguI GCTCTTC 1 cut(s) 418
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 2 cut(s) 378, 913
LweI GCATC 4 cut(s) 130, 398, 783, 801
MaeI CTAG 3 cut(s) 285, 342, 600
MalI GATC 7 cut(s) 165, 215, 400, 499, 536, 588, 663
MboI GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
MboII GAAGA 5 cut(s) 326, 405, 431, 525, 693
MfeI CAATTG 1 cut(s) 838
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 1 cut(s) 69
MlsI TGGCCA 2 cut(s) 800, 954
MluCI AATT 7 cut(s) 77, 172, 276, 289, 538, 627, 838
MluNI TGGCCA 2 cut(s) 800, 954
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 94, 584
Mox20I TGGCCA 2 cut(s) 800, 954
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 2 cut(s) 800, 954
MseI TTAA 2 cut(s) 575, 970
MslI CAYNNNNRTG 2 cut(s) 456, 641
Msp20I TGGCCA 2 cut(s) 800, 954
MspA1I CMGCKG 1 cut(s) 228
MspI CCGG 2 cut(s) 62, 204
MspR9I CCNGG 2 cut(s) 62, 561
MunI CAATTG 1 cut(s) 838
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 5 cut(s) 200, 363, 754, 868, 901
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 1 cut(s) 62
NcoI CCATGG 2 cut(s) 125, 219
NdeII GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
NlaIII CATG 6 cut(s) 129, 223, 455, 718, 868, 923
NlaIV GGNNCC 3 cut(s) 104, 202, 473
NmeAIII GCCGAG 1 cut(s) 346
NspI RCATGY 1 cut(s) 455
PciSI GCTCTTC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 3 cut(s) 347, 359, 887
PkrI GCNGC 3 cut(s) 230, 368, 903
Ple19I CGATCG 1 cut(s) 216
PleI GAGTC 1 cut(s) 331
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 331
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 3 cut(s) 104, 202, 473
PspPI GGNCC 2 cut(s) 718, 850
PsuI RGATCY 1 cut(s) 661
PvuI CGATCG 1 cut(s) 216
RsaI GTAC 3 cut(s) 55, 248, 473
RsaNI GTAC 3 cut(s) 54, 247, 472
RseI CAYNNNNRTG 2 cut(s) 456, 641
SapI GCTCTTC 1 cut(s) 418
SaqAI TTAA 2 cut(s) 575, 970
SatI GCNGC 3 cut(s) 229, 367, 902
Sau3AI GATC 7 cut(s) 163, 213, 398, 497, 534, 586, 661
Sau96I GGNCC 2 cut(s) 718, 850
ScaI AGTACT 1 cut(s) 248
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 62, 561
SduI GDGCHC 1 cut(s) 69
SfaNI GCATC 4 cut(s) 130, 398, 783, 801
SfoI GGCGCC 1 cut(s) 202
SinI GGWCC 2 cut(s) 718, 850
SmiMI CAYNNNNRTG 2 cut(s) 456, 641
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse9I AATT 7 cut(s) 77, 172, 276, 289, 538, 627, 838
SsiI CCGC 3 cut(s) 228, 426, 884
SspDI GGCGCC 1 cut(s) 200
SspMI CTAG 3 cut(s) 285, 342, 600
StyD4I CCNGG 2 cut(s) 60, 559
StyI CCWWGG 2 cut(s) 125, 219
TaqI TCGA 1 cut(s) 432
TasI AATT 7 cut(s) 77, 172, 276, 289, 538, 627, 838
TatI WGTACW 2 cut(s) 53, 246
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 3 cut(s) 347, 359, 887
Tru1I TTAA 2 cut(s) 575, 970
Tru9I TTAA 2 cut(s) 575, 970
TscAI CASTG 1 cut(s) 962
TseI GCWGC 2 cut(s) 366, 901
TspDTI ATGAA 3 cut(s) 198, 282, 687
TspRI CASTG 1 cut(s) 962
VpaK11BI GGWCC 2 cut(s) 718, 850
XapI RAATTY 1 cut(s) 276
XceI RCATGY 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 3 cut(s) 285, 342, 600
ZrmI AGTACT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.