MD09G1121300.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012328
Physical Location & Seq
Reverse (-)
9367672 .. 9368618
947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1121300.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGAAGTTGCAAGCCCTTTGAAAGACAGCTCCGTGTCGTCGAGTCCTTTCTCTTCTCCGAACGTCTCTGCCTTGCTCAAAACTCAAGAGACCGGTTTGCCTGTTGCGGTTAGTGTTCGAGTTGGCGGAAAGATCTTCAACCTCCACAAGTGGATACTTAACAAAAGATTGAACGAATCAAATGAGATTGATCTGCCACCGGGCTTTCCCGGAGGGCCAGAGACCTTTGAGATGATTGCACTCTTCATCTATGGCTCCTCCACATTGATTGATCCATTCAATGTGGTAGCCCTCAGATGTGCAGCAGAGTTTCTTGAGATGACGGAAGACTACTCCGGGAACCTCTGTGAGCGCTTCGATCTCTACTTAAACCAAGTGGTGATGCAGAGCTGGGATGACACCCTAATAGTGCTTCAAAAGTGCCAAACTTTGCTTCCATGGTCTGAAGACCTGCTCATTGTGAGCCGGTGCATAGAGTGCCTCGCCTTCATGGCCTGCATGGAGATCCTTGACCACGAAAGAAGGCATGAAACACCTGAGCTCACATTGGAAGCATTGGGCACGGGAAATTGGAGCTGCAAAACGGGGAGGGAAATTATGAATCAAGACCTTTGGATCAAGGATCTCATTGCGCTGCCATTCGGATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

217

Amino Acids

24.24

Weight (kDa)

4.59

Isoelectric Point (pI)

59.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012328)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48130
fragaria_vesca FvH4_6g41250 FvH4_6g41250 FvH4_6g41250
malus_domestica MD09G1121300.v1.1 MD17G1112300.v1.1
prunus_persica Prupe.3G202400_v2.0.a1
pyrus_communis pycom09g04570 pycom17g10400
rosa_chinensis RchiOBHm_Chr2g0156801
rosa_laevigata RLG00000020943
rosa_multiflora Rmu_co8389443.1_g000001
rosa_roxburghii Rroxscaffold_2G00092710
rosa_rugosa Rorug02G0456300
rosa_samantha Rh2AG521900 Rh2BG535300 Rh2CG506800 Rh2DG544300
rosa_wichuraiana Rw2G043090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 459
AciI CCGC 2 cut(s) 107, 126
AclWI GGATC 4 cut(s) 266, 499, 623, 630
AcuI CTGAAG 1 cut(s) 465
AfeI AGCGCT 1 cut(s) 353
AgeI ACCGGT 1 cut(s) 92
AgsI TTSAA 5 cut(s) 22, 139, 172, 280, 416
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
AluBI AGCT 4 cut(s) 30, 390, 541, 576
AluI AGCT 4 cut(s) 30, 390, 541, 576
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 3 cut(s) 70, 83, 215
AlwI GGATC 4 cut(s) 266, 499, 623, 630
Aor51HI AGCGCT 1 cut(s) 353
AoxI GGCC 2 cut(s) 215, 492
ApeKI GCWGC 3 cut(s) 302, 576, 634
AsiGI ACCGGT 1 cut(s) 92
AspLEI GCGC 2 cut(s) 354, 634
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 3 cut(s) 201, 210, 337
AsuHPI GGTGA 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 563
BanII GRGCYC 1 cut(s) 543
BbsI GAAGAC 2 cut(s) 333, 453
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 3 cut(s) 314, 563, 621
BciVI GTATCC 1 cut(s) 147
BcnI CCSGG 3 cut(s) 201, 210, 337
BcoDI GTCTC 3 cut(s) 70, 83, 215
BfoI RGCGCY 1 cut(s) 355
BfuAI ACCTGC 1 cut(s) 459
BfuI GTATCC 1 cut(s) 147
BglI GCCNNNNNGGC 1 cut(s) 491
BglII AGATCT 1 cut(s) 132
BisI GCNGC 3 cut(s) 303, 577, 635
BlsI GCNGC 3 cut(s) 304, 578, 636
Bme1390I CCNGG 3 cut(s) 201, 210, 337
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 2 cut(s) 256, 341
BmrFI CCNGG 3 cut(s) 201, 210, 337
BmsI GCATC 1 cut(s) 372
BpiI GAAGAC 2 cut(s) 333, 453
Bpu10I CCTNAGC 1 cut(s) 537
BpuEI CTTGAG 2 cut(s) 69, 335
BpuMI CCSGG 3 cut(s) 201, 210, 337
BsaI GGTCTC 2 cut(s) 83, 215
BsaJI CCNNGG 1 cut(s) 437
BsaWI WCCGGW 1 cut(s) 92
Bse118I RCCGGY 2 cut(s) 92, 465
Bse3DI GCAATG 1 cut(s) 627
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 1 cut(s) 400
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 2 cut(s) 307, 528
BseRI GAGGAG 1 cut(s) 247
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 3 cut(s) 314, 563, 621
BseYI CCCAGC 1 cut(s) 390
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 2 cut(s) 217, 494
BshTI ACCGGT 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 543
BsiSI CCGG 5 cut(s) 93, 200, 210, 336, 466
BsmAI GTCTC 3 cut(s) 70, 83, 215
BsmBI CGTCTC 1 cut(s) 70
BsnI GGCC 2 cut(s) 217, 494
Bso31I GGTCTC 2 cut(s) 83, 215
Bsp1286I GDGCHC 2 cut(s) 543, 563
Bsp143I GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
Bsp19I CCATGG 1 cut(s) 437
BspACI CCGC 2 cut(s) 107, 126
BspANI GGCC 2 cut(s) 217, 494
BspCNI CTCAG 2 cut(s) 306, 529
BspLI GGNNCC 2 cut(s) 256, 341
BspMI ACCTGC 1 cut(s) 459
BspPI GGATC 4 cut(s) 266, 499, 623, 630
BspTNI GGTCTC 2 cut(s) 83, 215
BsrDI GCAATG 1 cut(s) 627
BsrFI RCCGGY 2 cut(s) 92, 465
BssAI RCCGGY 2 cut(s) 92, 465
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
BssT1I CCWWGG 1 cut(s) 437
Bst6I CTCTTC 2 cut(s) 58, 248
BstAPI GCANNNNNTGC 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 13, 496
BstDEI CTNAG 2 cut(s) 293, 537
BstDSI CCRYGG 1 cut(s) 437
BstF5I GGATG 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 355
BstHHI GCGC 2 cut(s) 354, 634
BstKTI GATC 7 cut(s) 135, 193, 274, 361, 507, 618, 625
BstMAI GTCTC 3 cut(s) 70, 83, 215
BstMBI GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
BstMWI GCNNNNNNNGC 2 cut(s) 477, 491
BstSCI CCNGG 3 cut(s) 199, 208, 335
BstSLI GKGCMC 1 cut(s) 563
BstV1I GCAGC 3 cut(s) 314, 563, 621
BstV2I GAAGAC 2 cut(s) 333, 453
BstX2I RGATCY 3 cut(s) 132, 504, 622
BstYI RGATCY 3 cut(s) 132, 504, 622
BsuI GTATCC 1 cut(s) 147
BsuRI GGCC 2 cut(s) 217, 494
BtgI CCRYGG 1 cut(s) 437
BtsCI GGATG 1 cut(s) 400
BveI ACCTGC 1 cut(s) 459
Cac8I GCNNGC 2 cut(s) 13, 496
CfoI GCGC 2 cut(s) 354, 634
Cfr10I RCCGGY 2 cut(s) 92, 465
Cfr13I GGNCC 1 cut(s) 215
CspAI ACCGGT 1 cut(s) 92
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviAII CATG 4 cut(s) 438, 490, 499, 527
DdeI CTNAG 2 cut(s) 293, 537
DpnI GATC 7 cut(s) 134, 192, 273, 360, 506, 617, 624
DpnII GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
Eam1104I CTCTTC 2 cut(s) 58, 248
EarI CTCTTC 2 cut(s) 58, 248
EciI GGCGGA 1 cut(s) 141
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 543
Eco31I GGTCTC 2 cut(s) 83, 215
Eco47III AGCGCT 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 541
EcoT14I CCWWGG 1 cut(s) 437
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 437
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 4 cut(s) 441, 493, 502, 530
FaiI YATR 7 cut(s) 252, 439, 473, 491, 500, 528, 599
FatI CATG 4 cut(s) 437, 489, 498, 526
Fnu4HI GCNGC 3 cut(s) 303, 577, 635
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 3 cut(s) 303, 577, 635
GlaI GCGC 2 cut(s) 353, 633
GluI GCNGC 3 cut(s) 303, 577, 635
GsaI CCCAGC 1 cut(s) 394
HaeII RGCGCY 1 cut(s) 355
HaeIII GGCC 2 cut(s) 217, 494
HapII CCGG 5 cut(s) 93, 200, 210, 336, 466
HhaI GCGC 2 cut(s) 354, 634
Hin1II CATG 4 cut(s) 441, 493, 502, 530
Hin6I GCGC 2 cut(s) 352, 632
HinP1I GCGC 2 cut(s) 352, 632
HinfI GANTC 3 cut(s) 43, 176, 601
HpaII CCGG 5 cut(s) 93, 200, 210, 336, 466
HphI GGTGA 1 cut(s) 391
Hpy188I TCNGA 4 cut(s) 60, 296, 445, 644
Hpy188III TCNNGA 3 cut(s) 86, 314, 605
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 2 cut(s) 496, 516
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 7 cut(s) 11, 239, 302, 385, 471, 498, 579
HpyF10VI GCNNNNNNNGC 2 cut(s) 477, 491
HpyF3I CTNAG 2 cut(s) 293, 537
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 4 cut(s) 441, 493, 502, 530
HspAI GCGC 2 cut(s) 352, 632
Kzo9I GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
LmnI GCTCC 3 cut(s) 35, 260, 573
Lsp1109I GCAGC 3 cut(s) 314, 563, 621
LweI GCATC 1 cut(s) 372
MaeII ACGT 1 cut(s) 63
MalI GATC 7 cut(s) 134, 192, 273, 360, 506, 617, 624
MboI GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
MboII GAAGA 5 cut(s) 45, 127, 235, 338, 458
MflI RGATCY 3 cut(s) 132, 504, 622
MhlI GDGCHC 2 cut(s) 543, 563
MluCI AATT 2 cut(s) 568, 594
MlyI GAGTC 1 cut(s) 52
MnlI CCTC 7 cut(s) 152, 206, 268, 302, 353, 491, 582
MseI TTAA 3 cut(s) 159, 368, 649
MspI CCGG 5 cut(s) 93, 200, 210, 336, 466
MspR9I CCNGG 3 cut(s) 201, 210, 337
MwoI GCNNNNNNNGC 2 cut(s) 477, 491
NciI CCSGG 3 cut(s) 201, 210, 337
NcoI CCATGG 1 cut(s) 437
NdeII GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
NlaIII CATG 4 cut(s) 441, 493, 502, 530
NlaIV GGNNCC 2 cut(s) 256, 341
PfeI GAWTC 2 cut(s) 176, 601
PfoI TCCNGGA 2 cut(s) 208, 335
PinAI ACCGGT 1 cut(s) 92
PkrI GCNGC 3 cut(s) 304, 578, 636
PleI GAGTC 1 cut(s) 51
PpsI GAGTC 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 390
PspN4I GGNNCC 2 cut(s) 256, 341
PspPI GGNCC 1 cut(s) 215
PsuI RGATCY 3 cut(s) 132, 504, 622
SacI GAGCTC 1 cut(s) 543
SaqAI TTAA 3 cut(s) 159, 368, 649
SatI GCNGC 3 cut(s) 303, 577, 635
Sau3AI GATC 7 cut(s) 132, 190, 271, 358, 504, 615, 622
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 3 cut(s) 201, 210, 337
SduI GDGCHC 2 cut(s) 543, 563
SfaNI GCATC 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 84, 314
SmoI CTYRAG 2 cut(s) 84, 314
Sse9I AATT 2 cut(s) 568, 594
SsiI CCGC 2 cut(s) 107, 126
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 3 cut(s) 199, 208, 335
StyI CCWWGG 1 cut(s) 437
TaiI ACGT 1 cut(s) 66
TaqI TCGA 3 cut(s) 41, 118, 357
TasI AATT 2 cut(s) 568, 594
TfiI GAWTC 2 cut(s) 176, 601
Tru1I TTAA 3 cut(s) 159, 368, 649
Tru9I TTAA 3 cut(s) 159, 368, 649
TseI GCWGC 3 cut(s) 302, 576, 634
TspDTI ATGAA 4 cut(s) 235, 478, 543, 614
TspGWI ACGGA 2 cut(s) 22, 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.